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Previous issue date: 2010-12-03 / The trematode Echinostoma paraensei parasitizes the small intestine of rodents,
being its natural definitive host Nectomys squamipes. In spite of the great
importance of echinostomatides, there are few studies on morphology, biology and
physiology of E. paraensei and its interaction with their intermediate and definitive
hosts. In the present study 50 Rattus norvegicus (Wistar),adult females were used,
35 were individually infected with 150 E. paraensei metacercariae (Sumidouro
strain) and 15 were maintained uninfected, as control group. Weekly, seven infected
and three uninfected rodents were euthanized using CO2.The blood was collected to
hematological analysis, and serum obtained by centrifugation and used to aspartate
aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase
(ALKP), gamma-glutamyl transferase (GGT), bilirubin, glucose, total proteins and
fractions determinations. Fragments of liver were collected to glycogen
determination and histophatological analysis. Adult worms were loaded until third
week of infection, being the higher number of worms loaded at first week of
inection. The hepatocytes had rounded edges, with signs of impaired nuclear, mild
infiltration of polymorphonuclear and sinusoids slightly expanded. The hepatocytes
were vacuolated with discrete perivascular and periportal lymphocytic infiltration.
The serum biochemical parameters analyzed were increased at the first week of
infection, varying onward. Hematological analysis revealed the development of
normocytic and normocromic anaemia with anisocytic alteration. In the white blood
cells, only a leukopenia was observed at the third week of infection. The present
results are discussed. / O tremat?deo Echinostoma paraensei ? um parasito de intestino delgado de
roedores, sendo seu hospedeiro definitivo natural Nectomys squamipes. No presente
estudo, foram utilizadas 50 f?meas adultas de Rattus norvegicus (Wistar), 35
animais infectados individualmente com 150 metacerc?rias de E. paraensei
(linhagem Sumidouro) e 15 animais n?o infectados (controle). Semanalmente, sete
animais infectados e tr?s animais controle foram submetidos ? eutan?sia com CO2.
O sangue foi coletado para an?lise hematol?gica, e o soro obtido por centrifuga??o,
para as determina??es de aspartato aminotransferase (AST), alanina
aminotransferase (ALT), gamaglutamiltransferase (GGT), fosfatase alcalina
(ALKP), bilirrubina, glicose, prote?nas totais e fra??es. Fragmentos de f?gado foram
coletados para a determina??o de glicog?nio e para a an?lise histopatol?gica.
Vermes adultos foram recuperados at? a terceira semana de infec??o, sendo o maior
n?mro de vermes recuperados na primeira semana. Os hepat?citos apresentavam-se
com bordos arredondados e alguns sinais de comprometimento nuclear, infiltra??o
por polimorfonucleares nos sinus?ides, vacuoliza??o, discreta infiltra??o
linfoplasmocit?ria perivascular e periportal com poucas c?lulas mononucleares
presentes na luz do ducto biliar, alguns hepat?citos estavam hipotrofiados. As
an?lises hematol?gicas revelaram o desenvolvimento de uma anemia normoc?tica
normocr?mica, com anisocitose. N?o foram observadas altera??es significativas na
s?rie branca, havendo apenas uma leucopenia na terceira semana de infec??o. Os
resultados obtidos s?o discutidos.
| Identifer | oai:union.ndltd.org:IBICT/oai:localhost:jspui/2182 |
| Date | 03 December 2010 |
| Creators | Garcia, Juberlan Silva |
| Contributors | Silva, Jairo Pinheiro, Brandolini, Solange Viana Paschoal Blanco, Barros, Luciano Antunes |
| Publisher | Universidade Federal Rural do Rio de Janeiro, Programa de P?s-Gradua??o em Ci?ncias Veterin?rias, UFRRJ, Brasil, Instituto de Veterin?ria |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UFRRJ, instname:Universidade Federal Rural do Rio de Janeiro, instacron:UFRRJ |
| Rights | info:eu-repo/semantics/openAccess |
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