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Previous issue date: 2014-03-14 / Angiostrongylus cantonensis is a nematode of the Angiostrongylidae family. In humans, they can cause eosinophilic meningitis. Although the parasite have been described in 1935, little is known about the mechanisms of adaptation to its hosts. A. cantonensis owns molecules that help the parasite in the evasion of the host?s immune system, among these are SOD, catalase and HSP70. The main goal of this work was to study the profiles of HSP70, SOD and CAT of L5 produced in different hosts and analyze the diagnostic potential of the extracts. For the obtainment of fifth stage larvae (L5), rats and mice were infeceted with 104 and 50 third stage larvae, respectively, and euthanized after 21 days. The L5 were recovered from the brain of the animals and homogenized in different buffers to obtain the protein extracts. The quantification of the total protein suggests no significant difference between extracts. For the identification of HSP70, it was performed dot blot and Western blot, showing that in both samples HSP70 were present. ELISA assays were used for the quantification of HSP70 and analyze antigen-antibody binding with from individuals with confirmed diagnosis for angiostrongyliasis. L5 from usual hosts were recognized by the sera showing superior titles to the L5 from the accidental hosts. Levels of stress proteins HSP70, SOD (339U versus 0.08U of SOD/mg of protein) and catalase (184.4 versus 75.6 pmol of CAT mg-1 proteins) were higher p<0.05, in A. cantonensis larvae from usual hosts in comparison to the larvae obtained from accidental hosts. These data suggest that the levels of stress proteins may be associated to the adaptation of A. cantonensis to the usual hosts. / Angiostrongylus cantonensis ? um nemat?deo da fam?lia Angiostrongylidae. Em humanos, podem causar meningite eosinof?lica. Apesar de o parasito ter sido descrito em 1935, pouco se sabe dos mecanismos de adapta??o aos seus hospedeiros. A. cantonensis possui mol?culas que o auxilia na evas?o do sistema imune do hospedeiro, dentre estas est?o SOD, catalase e HSP70. O objetivo principal deste trabalho foi estudar o perfil de HSPs 70, SOD e CAT de L5 produzidas em diferentes hospedeiros e analisar o potencial diagn?stico dos extratos. Para a obten??o das larvas de quinto est?gio (L5), ratos e camundongos foram infectados com 104 e 50 larvas de terceiro est?gio, respectivamente, e eutanasiados ap?s 21 dias. As L5 foram recuperadas do c?rebro dos animais e homogeneizadas em diferentes tamp?es para obten??o dos extratos prot?icos. A quantifica??o de prote?nas totais sugere n?o haver diferen?as significativas entre os extratos. Para a identifica??o de HSP70 foram realizados dot blot e Western blot, mostrando que em ambas as amostras HSP70 estavam presentes. Ensaios de ELISA foram utilizados para a quantifica??o de HPS70 e para analisar a liga??o ant?geno anticorpo com soros de ind?viduos com diagn?stico confirmado para a angiostrongiliase, sendo que L5 provenientes de hospedeiros habituais apresentam reconhecimento pelos soros com t?tulos superiores ?s L5 provenientes de hospedeiros habituais. Os n?veis das prote?nas de estresse HSP70, SOD (339U versus 0,08U de SOD/mg de prote?na) e catalase (184,4 versus 75,6 pmole de CAT mg-1 prote?nas) foram superiores p<0,05, em larvas de A. cantonensis provenientes de hospedeiros habituais em compara??o com as larvas obtidas de hospedeiros acidentais. Estes dados sugerem que os n?veis de prote?nas de estresse podem estar associados a adapta??o do A. cantonensis aos hospedeiros habituais.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/275 |
| Date | 14 March 2014 |
| Creators | Rosa, Thaise Pa?m da |
| Contributors | Graeff-teixeira, Carlos |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Zoologia, PUCRS, BR, Faculdade de Bioci?ncias |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 2008925231902741151, 500, 600, 36528317262667714 |
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