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Previous issue date: 2016-03-02 / Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul - FAPERGS / Chronic kidney disease patients on peritoneal dialysis therapy are susceptible to infections, with peritonitis being the primary cause of technique failure. Peritoneal fluid culture is one of the essential elements for proper diagnosis and treatment of peritonitis. The aim of this study was to compare the time required to obtain a positive culture using different laboratory methods. An in vitro cross-sectional study comparing different laboratory techniques for preparation and culture of bacteria in peritoneal fluid. The research was conducted with 21 sterile dialysis fluid bags with 1.5% glucose concentration, and 21 peritoneal dialysis bags containing peritoneal fluid drained from patients without peritonitis, assisted at the Nephrology Unit from HSL-PUCRS. Fluids from the 42 peritoneal dialysis bags were contaminated by injecting a coagulase-negative Staphylococcus suspension and then prepared for culture using four distinct techniques - A (direct culture), B (post-centrifugation culture), C (direct culture after 4h sedimentation), and D (culture after 4h sedimentation and centrifugation) ? followed by seeding. In the 21 contaminated sterile bags, the mean times to obtain a positive culture with techniques D (19.6 h ?2.6) and C (19.1 h ?2.3) were longer in comparison to A (15.8 h ?3.0; p<0.01), but not statistically different from group B mean (19.0 h ?3.2). The same occurred in the 21 bags drained from patients, with mean times for techniques D (14.0 h ?1.9) and C (14.5 h ?1.7) being longer than technique A (12.22 h ?1.94; p<0.05), however not statistically different from technique B (13.2 h ?1.3). The sedimentation and centrifugation steps were unnecessary and may delay antibiotics sensitivity test result by approximately 8 hours. / Pacientes com doen?a renal cr?nica que realizam terapia de di?lise peritoneal est?o suscet?veis a infec??es, sendo peritonite a principal causa de fal?ncia do m?todo. A cultura do l?quido peritoneal ? um dos elementos essenciais para o manejo cl?nico e tratamento adequados da peritonite. O objetivo deste estudo foi comparar o tempo necess?rio para obter uma cultura positiva, com diferentes m?todos laboratoriais. Estudo transversal, in vitro, comparando diferentes t?cnicas laboratoriais de preparo e cultura para bact?rias em l?quido peritoneal. O estudo foi feito com 21 bolsas de l?quido de di?lise peritoneal est?reis, com concentra??o de 1,5% de glicose, e em 21 bolsas contendo l?quido peritoneal drenado de pacientes sem peritonite, atendidos pelo Servi?o de Nefrologia do HSL-PUCRS. O dialisado das 42 bolsas de di?lise peritoneal foi contaminado, injetando-se suspens?o de Staphylococcus coagulase negativa e, em seguida, submetido a quatro t?cnicas distintas ? A (cultura direta); B (cultura p?s-centrifuga??o); C (cultura ap?s sedimenta??o de 4 h); e D (cultura ap?s sedimenta??o de 4 h e centrifuga??o) ? de preparo e semeadura. Nas 21 bolsas est?reis contaminadas se verificou que as m?dias de tempo para positivar a cultura nas t?cnicas D (19,6 h ?2,6) e C (19,1 h ?2,3) foram maiores, comparadas ? A (15,8 h ?3,0; p<0,01), mas estatisticamente n?o diferentes da m?dia do grupo B (19,0 h ?3,2). O mesmo aconteceu nas 21 bolsas drenadas dos pacientes, com tempos m?dios para as t?cnicas D (14,0 h ?1,9) e C (14,5 h ?1,7) superiores ao tempo da t?cnica A (12,22 h ?1,94; p<0,05), por?m n?o estatisticamente diferente da t?cnica B (13,2 h ?1,3). As etapas de sedimenta??o e centrifuga??o foram desnecess?rias, podendo postergar em quase oito horas o resultado final da cultura, comparativamente ? cultura direta, atrasando o resultado do teste de sensibilidade aos antibi?ticos.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.pucrs.br:tede/6680 |
| Date | 02 March 2016 |
| Creators | Katzap, Roberta Monteiro |
| Contributors | Figueiredo, Carlos Eduardo Poli de, Figueiredo, Ana Elizabeth Prado Lima |
| Publisher | Pontif?cia Universidade Cat?lica do Rio Grande do Sul, Programa de P?s-Gradua??o em Medicina e Ci?ncias da Sa?de, PUCRS, Brasil, Faculdade de Medicina |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da PUC_RS, instname:Pontifícia Universidade Católica do Rio Grande do Sul, instacron:PUC_RS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 7620745074616285884, 600, 600, 600, 600, -8624664729441623247, -969369452308786627, -3614735573891122254 |
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