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Previous issue date: 2016-03-18 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Cereus jamacaruDC. is a species of Cactaceae used in folk medicine, popularly known as mandacaru. This study aimed to identify the metabolites classes quantify the phenolic content and flavonoids, evaluate the antioxidant activity, toxicity and pharmacological potential of chloroform fractions (FC) and hydromethanol (FHM) C. jamacaru root. The extracts were prepared by maceration and fractions are obtained by liquid-liquid partition. The classes of metabolites were detected by thin layer chromatography, in chemical developers. The content of phenolics, and flavonoids were determined by UV-vis spectrometry. The antioxidant activity was evaluated by the radical sequestration methods (DPPH) and ?-carotene/linoleic acid. Acute toxicity and the effect on the central nervous system by route rod test was evaluated. The antinociceptive activity was determined in tests of writhing, formalin and hot plate. The anti-inflammatory activity was carried out by the paw edema induced by carrageenan. The presence of terpenes, steroids, flavonoids, phenolic acids and amino acids was detected in the fractions. FC showed higher content of phenolics and flavonoids, and best antioxidant activity FHM analyzed by methods. Through the toxicological evaluation, it is suggested that the fractions are non-toxic orally. Regarding the antinociceptive activity, fractions reduced (p<0,001) the number of writhes and licking time in the 1? and 2? stage, at all doses tested. Fractions increased heat to the latency time (p<0,01 and p<0,001) at all doses and times observed. The anti-inflammatory activity was detected in fractions (p<0,001) in all the doses and times observed. The fractions did not affect the coordination of the animals, besides their antinociceptive and anti-inflammatory properties, with central action mechanism indicia, also showing the reduction effect of acute inflammatory processes. It is expected from this study, promote greater public awareness for the conservation and sustainable use of this species. / Cereus jamacaru DC. ? uma esp?cie de Cactaceae utilizada na medicina popular, conhecida popularmente como mandacaru.Este estudo teve como objetivo identificar as classes de metab?litos, quantificar o teor de fen?licos e flavonoides, avaliar a atividade antioxidante,toxidade e potencial farmacol?gico das fra??es clorof?rmica (FC) e hidrometan?lica (FHM) da raiz de C. jamacaru. Os extratos foram preparados por macera??o e as fra??esforam obtidas por parti??o l?quido-l?quido. As classes de metab?litos foram detectadas por cromatografia em camada delgada, em reveladores qu?micos. Os teores de fen?licos e flavonoides foram determinados por espectrometria no UV-vis. A a??o antioxidante foi avaliada pelos m?todos de sequestro do radical (DPPH) e ?-caroteno/?cido linoleico. Foi avaliado a toxidade aguda e o efeito sobre o sistema nervoso central pelo teste do rota rod. A atividade antinociceptiva foi apurada nos testes das contor??es abdominais, formalina e placa quente.A atividade anti-inflamat?ria foi realizada pelo teste do edema de pata induzido por carragenina. A presen?a de terpenos, esteroides, flavonoides, ?cidos fen?licos e amino?cidos foi detectada nas fra??es.A FC apresentou maior conte?do de fen?licos e flavonoides, e melhor atividade antioxidante que a FHM pelos m?todos analisados.Atrav?s da avalia??o toxicol?gica realizada, sugere-se que as fra??es s?o at?xicas por via oral. Em rela??o ? atividade antinociceptiva, as fra??es reduziram (p<0,001) o n?mero de contor??es e o tempo de lambida na 1? e 2? fase, em todas as doses testadas. As fra??es aumentaram o tempo de lat?ncia ao calor (p<0,01 e p<0,001) em todas as doses e tempos observados. A atividade anti-inflamat?ria foi evidenciada nas fra??es (p<0,001), em todas as doses e tempos observados.As fra??es n?o afetaram a coordena??o motora dos animais, al?m de possu?rem propriedades antinociceptivas e anti-inflamat?ria, com ind?cios de mecanismo de a??o central, apresentando tamb?m efeito na redu??o dos processos inflamat?rios agudos.Espera-se, a partir desse estudo, propiciar uma maior conscientiza??o da popula??o para a conserva??o e uso sustent?vel desta esp?cie.
| Identifer | oai:union.ndltd.org:IBICT/oai:tede2.uefs.br:8080:tede/416 |
| Date | 18 March 2016 |
| Creators | Santana, Amanda Ferreira |
| Contributors | Lucchese, Ang?lica Maria |
| Publisher | Universidade Estadual de Feira de Santana, Mestrado Acad?mico em Recursos Gen?ticos Vegetais, UEFS, Brasil, DEPARTAMENTO DE CI?NCIAS BIOL?GICAS |
| Source Sets | IBICT Brazilian ETDs |
| Language | Portuguese |
| Detected Language | English |
| Type | info:eu-repo/semantics/publishedVersion, info:eu-repo/semantics/masterThesis |
| Format | application/pdf |
| Source | reponame:Biblioteca Digital de Teses e Dissertações da UEFS, instname:Universidade Estadual de Feira de Santana, instacron:UEFS |
| Rights | info:eu-repo/semantics/openAccess |
| Relation | 8146646481622241874, 600, 600, 600, 600, 600, 5026123383450589282, 1571700325303117195, 700814650651154363, 3590462550136975366 |
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