Numerous studies have focused on identifying novel probiotic-based treatment options for immune homeostasis maintenance and favorable modulation of the gut microbiota which acts as a key regulator of the gut-brain axis. Recently, probiotics interventions are gaining interest as effective approaches to treat neuropsychiatric disorders through the gut-brain axis. However, there is limiting knowledge about probiotics' effects during puberty on the developing brain and immune responses. Probiotic intake could offer a strategy to counteract the immune, microbial and behavioral disturbances induced by inflammatory LPS. Thus, we hypothesized that the intake of a novel probiotic bacterium Rouxiella badensis subsp. acadiensis would modulate the immune response and that pubertal administration could mitigate LPS- induced inflammation and prevent enduring behavioral changes later in life.
We investigated the interaction of the probiotic with the intestinal mucosa and its ability of modulating the gut mucosal immunity (Article 1). Next, we examined the ability of pubertal treatment with R. badensis subsp. acadiensis to alleviate LPS-induced anxiety-like and depression-like behaviors in adult male and female mice and to affect the expression of 5HT1A receptors in specific brain areas of adult mice (Article 2). We finally studied the ability of R. badensis subsp. acadiensis treatment during puberty to mitigate the effects of LPS on the immune system and on the gut microbiome composition (Article 3). These studies have demonstrated the ability of R. badensis subsp. acadiensis to survive the gastrointestinal conditions, interact with the gut epithelium and modulate the intestinal homeostasis. Pubertal use of the bacterium was associated with sex-specific effects on the acute immune response, microbiome structure, enduring neurobehavioral outcomes and the expression of 5HT1A receptors in specific brain areas, later in life. This dissertation emphasizes on the importance of puberty as a window of opportunities during which probiotic use can alleviate the long-term neural, behavioral, immunological and microbiome alterations induced by stress.
| Identifer | oai:union.ndltd.org:uottawa.ca/oai:ruor.uottawa.ca:10393/44293 |
| Date | 22 November 2022 |
| Creators | Yahfoufi, Nour |
| Contributors | Matar, Chantal, Ismail, Nafissa |
| Publisher | Université d'Ottawa / University of Ottawa |
| Source Sets | Université d’Ottawa |
| Language | English |
| Detected Language | English |
| Type | Thesis |
| Format | application/pdf |
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