Recently there has been more critical attention given to the violent role of the North West Mounted Police (NWMP) in the unfolding of settlement and colonial laws in western Canada. However, few have offered a comprehensive analysis of the violent encounters that are recorded (and missing) in the archival records and correspondence of the NWMP, and other secondary sources. Similarly, few researchers have utilized the ‘past’ experiences of Aboriginal peoples to try and understand the ongoing chasm today between non-indigenous settlers and Aboriginal peoples of Canada. In making the “marginal central” (Fitzpatrick 1989), and simultaneously challenging the dominant colonial narrative, I offer a socio-historical analysis of western Canada during the NWMP era (1873-1905), to show how it was (and still is), like other colonial frontiers, a violent space and time. I explore this argument by situating the violent encounters between the NWMP, white settlers, and Aboriginal peoples within the colonial relations that were structured to maintain the marginalization and dispossession of Aboriginal peoples. Failing to recognize and resist this part of western Canadian history, and the underlying logic behind it, is denial and limits the rationality and potential of non-indigenous Canadian populations to work for, and even conceive of, achieving an authentic reconciliation with Aboriginal peoples.

In working with Elder John Killulark, this project aimed at documenting the history of the Amaruq and Kigjugalik families and his perspective of the Land around Akulliqpaaq Qamaniq (Aberdeen Lake) and Qamaniq Tugliqpaaq (Schultz Lake). The study area is on the west side of Hudson Bay, Nunavut, and has been used by the families for many generations. In sharing his thoughts about life prior to moving to the permanent settlement of Baker Lake. He then described the family’s homeland through stories, songs, and legends and by providing a detailed map of the area including more than 290 place names. Our parents, grandparents, great-grandparents, and ancestors were once closely linked to the Land. The Land is important to Inuit and through this project, we can reflect on how old and new traditions are coming together to provide a bright future for Inuit.

Chlamydia, caused by the bacterium Chlamydia trachomatis, is one of the most important sexually-transmitted infections globally. In addition to accounting for millions of cases every year, the disease causes numerous irreversible complications such as chronic pelvic pain, infertility in females and pelvic inflammatory disease. This thesis presents a number of mathematical models, of the form of deterministic systems of non-linear differential equations, for gaining qualitative insight into the transmission dynamics and control of Chlamydia within an infected host (in vivo) and in a population. The models designed address numerous important issues relating to the transmission dynamics of Chlamydia trachomatis, such as the roles of immune response, sex structure, time delay (in modelling the latency period) and risk structure (i.e., risk of acquiring or transmitting infection). The in-host model is shown to have a globally-asymptotically stable Chlamydia-free equilibrium whenever a certain biological threshold is less than unity. It has a unique Chlamydia-present equilibrium when the threshold exceeds unity. Unlike the in-host model, the two-group (males and females) population-level model undergoes a backward bifurcation, where a stable disease-free equilibrium co-exists with one or more stable endemic equilibria when the associated reproduction number is less than unity. This phenomenon, which is shown to be caused by the re-infection of recovered individuals, makes the effort to eliminate the disease from the population more difficult. Extending the two-group model to incorporate risk structure shows that the backward bifurcation phenomenon persists even when recovered individuals do not acquire re-infection. In other words, it is shown that stratifying the sexually-active population in terms of risk of acquiring or transmitting infection guarantees the presence of backward bifurcation in the transmission dynamics of Chlamydia in a population. Finally, it is shown (via numerical simulations) that a future Chlamydia vaccine that boosts cell-mediated immune response will be more effective in curtailing Chlamydia burden in vivo than a vaccine that enhances humoral immune response. The population-level impact of various targeted treatment strategies, in controlling the spread of Chlamydia in a population, are compared. In particular, it is shown that the use of treatment could have positive or negative population-level impact (depending on the sign of a certain epidemiological threshold).

INTRODUCTION: Autophagy is a regulated degradation pathway functioning in both cell survival and cell death. Its role in cancer is controversial since autophagy can be protective or destructive to tumor cells, depending on individual genetic signatures, stage of malignancy and treatment conditions. Hypoxia is a common feature of solid tumors, correlating with poor prognosis and chemoresistance. We have investigated autophagy in hypoxic cancer cells and examined the role of the hypoxia-inducible protein, BNIP3. METHODS: Multiple cancer cell lines were exposed to chronic hypoxia (<1% O2) in the presence or absence of specific inhibitors for autophagy and apoptosis. Cell death was measured by membrane permeability assay, and autophagy was assayed by GFP-LC3 distribution, LC3 processing, electron microscopy, and acidic vacuole formation. BNIP3 was over-expressed by transient transfection, stably induced in a tetracycline-regulated expression system, or knocked down using siRNA. Whole brain morphology, cell proliferation, and hypoxic response were additionally studied in a BNIP3-null mouse model. RESULTS: Autophagic cell death was detected in hypoxic cancer cells, occurring independent of apoptosis through a mechanism involving BNIP3. BNIP3 itself induced autophagic cell death, and loss of BNIP3 protected against hypoxia-induced autophagy and cell death. Loss of BNIP3 also resulted in differential growth and cell cycle progression in vitro, and increased brain cellularity in vivo compared to wild type controls. Potential mediators of resistance to BNIP3-induced cell death were identified using a novel model of BNIP3 resistance. CONCLUSIONS: Taken together, these results support the emerging theory that autophagy represents an alternative cell death pathway that could be targeted in hypoxic and/or apoptosis-resistant tumors. We have specifically identified BNIP3 as a potential target molecule in this pathway. Finally, we have identified a possibly novel role for BNIP3 in brain development and cell cycle regulation. These findings have important clinical applications given the potential for personalized cancer therapy based on individual tumor characteristics including autophagic capacity, hypoxic status, and BNIP3 activity.

In most animals, oogenesis involves a syncytial “cyst” stage. Cysts are produced by incomplete mitotic divisions of gonial precursor cells, leaving the resulting cystocytes interconnected by cytoplasmic bridges. The bridges subsequently break down, liberating the developing gametes. In some animals (e.g. meroistic insects) cysts are “polarized”, such that certain cystocytes differentiate as supportive nurse cells, rather than oocytes. The variability of cysts in animal oogenesis contrasts with the relative universality of spermatogenic cysts, making the functional importance of cysts in oogenesis unclear. I have studied oogenesis in a polychaete worm, Ophryotrocha labronica (Annelida: Dorvilleidae). These worms produce polarized, two-celled oogenic cysts with one nurse cell and one oocyte. Such cysts resemble their better-characterized counterparts in meroistic insects. However, using a variety of light- and electron-microscopic techniques, I show here that the resemblance between O. labronica and meroistic insects is largely superficial. Rather, the roles of nurse cells and the mechanisms underlying cystocyte differentiation are quite distinct in both groups. Therefore, similarities between these polychaetes and insects are probably examples of convergent evolution rather than homology. These observations underscore the plasticity of oogenesis among animals. Mechanisms by which germ cells become distinct from somatic cells in animals are also a subject of considerable research activity. Two general modes of germ-cell specification have been described in animals: deterministic specification, which is typical of established model species (e.g., Drosophila melanogaster and Caenorhabditis elegans) and inductive specification, which, though it is the more-common mode among animals, has not been well studied. As an annelid worm, O. labronica likely specifies its germ cells inductively, and therefore has potential to serve as a model species for studies of inductive germ cell specification. Realizing this potential, however, will require the development of genetic resources for this species. I describe the beginnings of such work here: the isolation and characterization of a vasa/PL10-like gene whose expression is largely restricted to germ cells, the construction of a cDNA library, and the refinement of methods for in situ hybridization and immunostaining to visualize gene expression in whole worms.

The purpose of this research program was to measure the efficiency of evacuated tube solar collectors under various operating conditions including: the angle of inclination towards the incident solar radiation, heat transfer fluid flow rate, glazing installation, and number of evacuated tubes. The operating conditions and configurations were chosen to represent realistic or probable installation scenarios and environmental conditions. Furthermore, the research aimed to identify the suitability of evacuated tube solar collectors to each of the scenarios. The scope of the research project was limited to the efficiency of a single tube, and various configurations of a 32-tube panel, not the entire solar domestic hot water or space heating system. Thus, factors such as heat loss in the tubing, solar storage tank, and heat exchanger efficiency were not investigated. The findings indicated that efficiency varied by approximately 5% in general between the different collector configurations.

A Web-based teaching tool called WebCAPSI was modified and tested as an online treatment for social anxiety. Two-Hundred and Seventy-Seven Introductory Psychology students at the University of Manitoba participated in the study. Twenty-eight participants were excluded from the study, resulting in 121 participants in the control group and 128 in the treatment group used for the final analysis. All participants received written materials on treatment for social phobia via WebCAPSI; however, the material was broken down into discrete units with assigned study questions for participants in the treatment condition. Participants in the treatment condition answered specific questions within the WebCAPSI program whereas participants in the control condition answered questions unrelated to the content of the materials. Further, participants in the treatment condition were given the opportunity to serve as peer reviewers. Results of this study indicated significant differences in post-treatment anxiety scores on two anxiety measures between groups, higher treatment expectancy scores in the treatment group, and higher baseline anxiety scores predicting greater reduction in anxiety post-treatment. Peer review did not appear to have a significant effect on post-treatment anxiety scores. These results indicate that the WebCAPSI program may be a useful tool to present information on the treatment of social anxiety.

One technique used to control the influx of renewable generation while maintaining competitive principles is a Request for Proposals (RFP). However, lack of transmission planning due to a disconnection between generation and transmission owners, difficulty in identifying viable projects, and high risk for proponents stand as obstacles to the goals of an RFP. This research proposes a procedure which minimizes the effect of these obstacles; meeting the purchaser requirements for low price and combining conventional planning concepts with feedback from competitive structures. The general features of the method include definition of generation limits and study area, expansion plan design, transmission cost evaluation, optimal selection of requested generation levels, and final selection of successful proponents. A case study using a real system in New Mexico demonstrates these concepts.

During the Cold War, war by proxy was a key strategy of indirect conflict between the United States and the Soviet Union. The purpose of these proxy wars was to either maintain or change the balance of power between the superpowers/great powers in conflict areas outside the central front in Europe. Within the condition of Mutual Assured Destruction (MAD), both the United States and the Soviet Union sought to avoid direct confrontation between their conventional military forces in regional conflicts out of a fear that it would escalate to an all out nuclear war.

Background: The purpose of this secondary data analysis was to determine the prevalence of sexually transmitted and blood-borne infections (STBBI) and to examine the role of survival sex in sexual risk among street-involved youth in Winnipeg, Manitoba. Methods: Between 1999 and 2003, 743 Winnipeg street youth were interviewed in three separate cohorts as part of a national multi-centred study by the Public Health Agency of Canada. Results: Among the 673 Winnipeg street youth who responded to questions relating to survival sex, 26.3% reported they had engaged in survival sex. Females and Aboriginal youth were at greatest risk for sex trade involvement. Youth engaged in survival sex were diagnosed with significantly higher rates of STBBI; experienced greater amounts of abuse; and were at greater risk for participating in high risk sexual behaviours compared to their non-sex trade counterparts. Conclusion: Gender specific, culturally appropriate, and youth oriented prevention and intervention strategies are urgently required to reduce the prevalence of STBBI and survival sex among this at-risk population.