This project examines the landscape of the farming community of Beagle, Oregon prior to and during the Second World War and the effect on it due to the construction of Camp White, a World War II training facility. The Beagle landscape is examined through the prism of current theory that suggests that landscapes are not discrete units of analysis but are, instead, symbiotic relationships between land and people. Utilizing archives, contemporary newspaper accounts, photographs, oral histories, and archaeological investigation, the history of the construction of Beagle landscape, the effects of the construction of Camp White, the subsequent removal of Beagle residents, and postwar renewal are examined. The project concludes that the Beagle landscape was, and is, a holistic entity that, though dramatically changed in 1942, continues to exist and inform the lives of surviving original residents as well as the history of the Beagle area. / Graduation date: 2007

Zugl.: Regensburg, Univ., Diss. / Includes bibliographical references (p. 158-164).

During the New Deal, the Farm Security Administration (FSA) developed what is arguably one of the most provocative and far-reaching programs for farm workers undertaken by the U.S. federal government to date. Through the Migratory Labor Camp Program the FSA promised to efficiently funnel workers to fulfill the agricultural industry’s labor demands while providing migrants modern, up-to-date housing and services to alleviate the well-documented substandard conditions many faced. Most scholars have analyzed the camps primarily as sites of labor, capital, and state regulation. Rather than view the camp program as simply a government effort to more efficiently coordinate the nation’s farm labor market, this study argues that the services, programs, and activities FSA officials administered in the camps sought to regulate and transform significant and often intimate social and cultural aspects of migrants’ daily lives. By examining the role of the camps’ architecture, medical clinics, nurseries and elementary schools, as well as the “self-governing” camp committees and councils, this dissertation engages in a gendered analysis of labor to reveal how the federal camps were unique dual-purpose domestic and labor spaces. Analyzing the camps as simultaneous productive and reproductive sites allows us to see them as part of a contested terrain in which complex issues of identity, community, citizenship, and labor were negotiated on a daily basis, affecting U.S. farm labor and race relations well beyond the perimeters of the federal camps. / text

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal

This creative project examined design and management alternatives for the Hardin Ridge Recreation Area located in the Hoosier National Forest. The design intent of the project was to develop Hardin Ridge into a wildland recreation facility which offered visitors a variety of experiential opportunity and choice, and provided experiences which could effectively accommodate a demographically diversified population. This project would also provide environmental educational opportunities and attempt to retain the integrity of the sites natural systems. An examination of the importance and benefits of outdoor recreation, National Forest Service recreational planning, outdoor recreation demographic diversity and outdoor recreation design issues was conducted in formulating the projects design criteria and theory. This design criteria and theory was then utilized to create the design concepts directly applicable to the Hardin Ridge project site and develop a design ideology applicable to most U.S. wilderness recreation areas. The final product of the study was a design and management plan which effectively addressed the design objectives at Hardin Ridge and created a design model capable of meeting similar design objectives at the national level. / Department of Landscape Architecture

Le conflit israélo-palestinien dure depuis plus de 60 ans. Non seulement perdure-t-il, il gagne aussi en complexité. Cette thèse s’intéresse à l’expérience d’exil des Palestiniens et plus particulièrement à celle de femmes palestiniennes vivant en camp de réfugiés au Liban. La mémoire palestinienne a longtemps été, dans son ensemble, occultée dans la littérature, et qui plus est l’expérience des femmes; la façon dont leurs récits sont construits nous le démontre bien. La présente étude s’inscrit donc dans la lignée de travaux qui font une place aux « voix silencieuses » que sont souvent celles des femmes réfugiées palestiniennes des camps. Cette thèse s’appuie sur une approche qualitative – récits de vie et observation participante – et fait suite à une recherche qui a été menée entre 2009 et 2011 dans le camp palestinien de Bourj El Barajneh au Liban. Les résultats dégagés confirment que, dans l’exil, une partie de l’expérience de la nakba palestinienne telle que vécue par les femmes s’est perdue. Ceci dit, si la quasi-absence des femmes caractérise l’exode, on voit ces dernières s’affirmer au fil de l’exil qui devient une réalité durable. Au cours des deux premières décennies, les femmes apparaissent comme des « résistantes du quotidien ». Puis, la montée du sentiment national palestinien et l’éclatement de la guerre civile libanaise amènent les femmes à investir de plus en plus l’espace public. En temps de guerre, toutes les femmes participent à la survie de la communauté, et cela, par l’extension de leurs tâches domestiques et sociales. Plus le conflit prend de l’ampleur, plus leurs activités se diversifient : elles intègrent d’autres tâches à celles qui leur sont traditionnellement assignées. À l’issue du conflit, une grande partie des femmes palestiniennes commencent à prendre leurs distances de la lutte nationale partisane. Pour plusieurs d’entre elles, la fin de la guerre est aussi la fin des illusions : elles ont le sentiment d’avoir été abandonnées par la classe politique. Ainsi, le mouvement nationaliste palestinien a certes bousculé les rôles de genre, mais il n’a pas permis d’induire des changements durables. Dans les récits des femmes, on voit qu’à travers l’exil s’est créé un lien avec ce milieu que l’on croyait temporaire, le camp de Bourj El Barajneh : un lien qui se situe au cœur d’une tension entre un pôle réel et un pôle symbolique. Le camp « réel » est décrit comme insalubre, instable et non sécuritaire, et la vie dans ce camp est à ce point précaire et difficile que les femmes s’accrochent à cet autre camp qui, lui, est porteur de mémoire, de souvenirs, de relations et de rêves. C’est d’ailleurs parce que ce second pôle existe que la vie dans le camp peut être tolérée. Si la lutte nationale a été pour une certaine génération de Palestiniennes la préoccupation première, la fin de la guerre signe la perte de vitesse de cette lutte qui s’est longtemps avérée structurante. Ceci dit, le modèle de résistance, lui, persiste. Les femmes continuent de lutter et apparaissent comme des « actrices de la transmission ». L’un de ces projets qu’elles font leur, la transmission de l’identité religieuse, prend rapidement de l’ampleur alors que la communauté palestinienne peine à se relever des affres de la guerre. Nombreuses sont les femmes qui cherchent un sens à la vie dans ce cumul de catastrophes, et la religion les soutient dans cette quête, mais en plus c’est à travers elle que le projet du retour en Palestine est porté. D’ailleurs, la mémoire de la Palestine est une autre valeur que les femmes cherchent à transmettre d’une génération à l’autre. Maintenir la mémoire de la Palestine est un rôle traditionnel de la femme palestinienne. Ceci dit, les femmes ne remplissent pas ce rôle « aveuglément » : elles transmettent une mémoire, un message qu’elles ont cherché, reconstruit, évalué et parfois critiqué. Enfin, un autre projet se manifeste rapidement dans l’exil : la transmission des connaissances, une valeur phare pour les Palestiniennes puisque à la fois stratégie de survie, de développement et d’ascension sociale. Mais pour quelques-unes, l’éducation est une lutte parce que confrontée à des contraintes contextuelles et au poids des traditions. Ainsi, c’est par des valeurs traditionnellement portées et transmises par les femmes – l’identité religieuse, la mémoire et l’éducation – que l’oppression et la colonisation des Palestiniens se combattent au quotidien. / The Israeli-Palestinian conflict has lasted more than 60 years and persists not only in time but also in complexity. This thesis focuses on the Palestinian exile and particularly, the experience of exile of Palestinian women living in refugee camps in Lebanon. Palestinian memory has for a long time been occulted in the literature and specifically, the experience of women and how their stories are constructed by gender. The present study is therefore in a line of work that gives a place to these “silent voices” that are often those of the Palestinian women of the camps. This research is based on a qualitative methodology – life stories and participant observation –, research that took place between 2009 and 2011 in the refugee camp of Bourj El Barajneh in Lebanon. The results show that, in exile, a part of the Palestinian nakba experienced by women, has been lost. That said, if a virtual absence of women characterizes the exodus, over exile, women become more assertive. During the first two decades in exile in Lebanon, women appear as “everyday resistant”. Then, the rise of a national sentiment which was rapidly followed by the outbreak of the Lebanese civil war, made women more and more present in the public space. Indeed, in wartime, all the women were involved in the community’s survival, and that, by an extension of their domestic and social roles. The longer the conflict lasts, the more diverse are their activities: it includes other tasks than those traditionally assigned to them. At the end of the conflict, a large part of Palestinian women are beginning to distance themselves from the national struggle. For many, the end of the war also means the end of illusions: they feel they have been abandoned by the political class. Thus, if the Palestinian nationalist movement has certainly brought changes in gender roles, it has failed to bring about lasting changes. Also, in the women's narratives, we see that in time, a bond is created with the space “Bourj El Barajneh camp”, a, bond that is located in a tension between two poles. First, there is a “real pole” where the camp appears as unsafe and unstable. Second, life in the camp is so precarious and difficult that women cling to another pole, a “symbolic pole” which represents the camp as a bearer of memories, relationships and dreams. And it’s because this last pole exists that life in the camp can be tolerated. If the Palestinian national struggle – for a certain generation of Palestinian women – was the main struggle, the end of the war signed “the end of illusions” and the slowing of the national struggle which has long proven structuring. That said, the pattern of resistance persists while women continue to resist and appear as “actresses of transmission”. The transmission of religious identity quickly gained in importance as the Palestinian community struggled to recover from the horrors of war. Through religion, many women found meaning in a life and it is also through religion that the return to Palestine is now carried. Moreover, the memory of Palestine is another value that women seek to pass on from a generation to another. Even though, passing on the memory of Palestine is a role traditionally carried by women, they do not fulfill it “blindly” but they convey a message that has been sought, rebuilt and sometimes criticized. Finally, another project arrives rapidly in exile: the transmission of knowledge, a core value for Palestinian women as it is a strategy for survival, development and social mobility. But for some, because faced with contextual constraints and the weight of tradition, education is still a struggle. Thus, it is because women carry and transmit traditional values – religious identity, memory and education – that the oppression and colonization of Palestinians can be fought everyday.

Thesis (doctoral)--Heinrich-Heine-Universität, Düsseldorf, 2002. / Includes registers of names of SS-Baubrigaden inmates, including those who died (p. 338-353); and names of SS tried for crimes (p. 357-363). Includes lists of SS-Baubrigaden and commandants (p. 335-337), and a list of memorials dealing with SS-Baubrigaden (p. 364-367). Includes bibliographical references (p. 377-399) and indexes.

Submitted by Luciana Ferreira (lucgeral@gmail.com) on 2016-08-04T11:29:03Z No. of bitstreams: 2 Dissertação - Mirian Vieira Teixeira - 2016.pdf: 1440228 bytes, checksum: a429eb9e9ac048fbcc06d59fca852c03 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2016-08-04T11:30:39Z (GMT) No. of bitstreams: 2 Dissertação - Mirian Vieira Teixeira - 2016.pdf: 1440228 bytes, checksum: a429eb9e9ac048fbcc06d59fca852c03 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2016-08-04T11:30:40Z (GMT). No. of bitstreams: 2 Dissertação - Mirian Vieira Teixeira - 2016.pdf: 1440228 bytes, checksum: a429eb9e9ac048fbcc06d59fca852c03 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-03-10 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG / The Paracoccidioides genus comprises a complex of pathogenic fungi that are the etiologic agents of paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in Latin America. The infection begins after inhalation of fungal propagules, which reach the epithelium of the alveoli where the transition from the mycelial to the pathogenic yeast form. Host elevated temperature triggers the morphological switch, which is necessary for fungal pathogenicity. The cAMP/protein kinase A (PKA) signaling pathway has been shown to be important in controlling morphological changes and the pathogenicity of several pathogenic fungi. Evidence also highlights the importance of the cAMP/PKA pathway in the morphological transition of Paracoccidioides. PKA is the major effector of this signaling pathway. The protein is an inactive tetramer composed of regulatory subunit, encoded by the BCY1 gene; and catalytic subunit, encoded by the TPK2 gene. Upon binding of cAMP to the regulatory subunits, the catalytic subunits dissociate and become active. Activated PKA subsequently phosphorylates protein kinases, transcription factors, and other substrates to control several biological processes. In this study, we evaluated the expression and interactions of Tpk2 protein Paracoccididioides spp. The Tpk2 is present in mycelium decreased during the initial stages of transition phases, and increases again at the end of differentiation, with maximal levels in yeast. We analyzed the interactions of recombinant Tpk2p with Paracoccidioides proteins using pull-down assays followed by MS analysis. Two interacting proteins were identified: the heat shock protein 90 (Hsp90) and a conserved hypothetical protein with a MFS domain. Hsp90 is involved in the regulation of morphogenesis, development and virulence in several thermal dimorphic fungi. These data are important for understanding the mechanisms that trigger the transition phases in Paracoccidioides. / O gênero Paracoccidioides compreende um complexo de fungos patogênicos, que são os agentes etiológicos da paracoccidioidomicose (PCM), a micose sistêmica mais prevalente na América Latina. A infecção inicia-se com a inalação de propágulos do fungo, que atingem o epitélio dos alvéolos pulmonares, onde ocorre à transição da forma de micélio para a forma patogênica, de levedura. Há evidências de que a temperatura seja o principal fator responsável pela diferenciação celular desses fungos, e sua patogenicidade é frequentemente associada com a transição dimórfica. A via de sinalização cAMP/ proteína quinase A (PKA) controla alterações morfológicas e de virulência/patogenicidade em várias espécies de fungos patogênicos humanos. Evidências apontam também para a importância da via cAMP/PKA em Paracoccidioides spp. A PKA é o principal efetor desta via de sinalização. A proteína na forma inativa é um tetrâmero composto de subunidade regulatória, codificada pelo gene BCY1; e subunidade catalítica, codificada pelo gene TPK2. Após a ligação de cAMP às subunidades regulatórias, as subunidades catalíticas dissociam-se e tornam-se ativas. Ativada a PKA fosforila proteína-quinases, fatores de transcrição, e outros substratos para controlar diversos processos biológicos. Neste estudo, avaliamos a expressão e as interações da proteína Tpk2 de Paracoccididioides spp. A Tpk2 está presente em micélio, diminui nos estágios iniciais da transição de fases e volta a aumentar no final da diferenciação, apresentando níveis máximos na levedura. Foram analisadas as interações de Tpk2p recombinante com proteínas de Paracoccidioides utilizando ensaios de pull-down, seguido por análise de MS. Foram identificadas duas proteínas que interagem: a proteína de choque térmico 90 (Hsp90) e uma proteína hipotética conservada com um domínio MFS. Hsp90 está envolvido na regulação da morfogênese, desenvolvimento e virulência em vários fungos dimórficos térmicos. Estes dados são importantes para entendimento dos mecanismos que disparam a transição de fases em Paracoccidioides spp.

Introduction: 23 million patients worldwide suffer from heart failure. These patients depend on cardiac research, because cardiac research enables the development of new therapeutic strategies and –targets. In cardiomyocytes, the compartmentalization of cAMP and cGMP depends on many factors. T-tubuli and PDEs are responsible for the division of cells in microdomains in which localized and specific cAMP and cGMP-signaling occurs. The aim of this thesis was to develop a method to answer the open questions that remain about the physiological and pathophysiological significance of cAMP/cGMP compartmentalization. Methods: I used the zebrafish as a model, because the transparency of zebrafish larvae enabled non-invasive fluorescent imaging in cardiomyocytes in the living animal. I cloned the Fluorescence Resonance Energy Transfer (FRET) sensors EPAC1-camps for cAMP and cGi500 for cGMP and injected them into zebrafish fertilized embryos. Then I used the F0 generation for Fluorescence Lifetime Imaging (FLIM) -FRET-measurements of cAMP and cGMP. Ca2+ is an important downstream mediator of cAMP and cGMP, because Ca2+ regulates cardiac contraction. Therefore, I also cloned the Ca2+ sensor GCaMP6 and used the dye Fluo-4 AM to include intracellular Ca2+ in the imaging. Results: The cloned sensors for cAMP, cGMP and Ca2+ were successfully injected into the zebrafish and showed expression in individual cardiomyocytes. I developed a protocol to mount the living zebrafish embryos and to measure intracellular cAMP and cGMP with FLIM-FRET in vivo with high spatial resolution. I characterized the sensors in their functionality by showing that the sensors react to changes in intracellular concentrations of cAMP and cGMP. The results of this study include evidence that zebrafish have mechanisms that lead to cAMP/cGMP compartmentalization in the absence of T-tubuli, and these mechanisms keep compartmentalization constant even under extreme cAMP or cGMP increasing drug treatment. Furthermore, I imaged intracellular Ca2+ by confocal microscopy and developed a protocol to use Fluo-4 AM for Ca2+ imaging. Conclusion: The method used in this thesis should allow the investigation of subcellular cAMP/cGMP compartmentalization and Ca2+ and to subsequently answer open questions in the field, for example whether a change of cAMP compartmentalization leads to the pathological phenotypes of cardiac disease or if a changed compartmentalization of cAMP in cardiac disease influences Ca2+ concentrations and therefore contraction. Additionally, this method can be used to learn more about cAMP, cGMP und Ca2+ during regeneration in the heart, because the zebrafish cardiomyocytes can regenerate. / Einleitung: Weltweit sind mehr als 23 Millionen unter Herzinsuffizienz leidende Patienten auf die kardiologische Grundlagenforschung angewiesen, da diese die Voraussetzung für eine bessere Versorgung durch adaptierte und neue Behandlungswege schafft. In Kardiomyozyten hängt die Kompartimentierung von cAMP und cGMP von vielen Faktoren ab. T-Tubuli und PDEs werden unter anderem für die Aufteilung der Zellen in Mikrodomänen, in denen lokalisierte und spezifische cAMP- und cGMP-Signalgebung stattfinden kann, verantwortlich gemacht. Das Ziel dieser Arbeit war die Etablierung einer Methode, mithilfe derer offene Fragen bezüglich der physiologischen und insbesondere der pathophysiologischen Relevanz der cAMP- und cGMP Kompartimentierung beantwortet werden können. Methode: Als Modell diente der Zebrafisch, da die Transparenz von Zebrafisch Embryonen eine nicht-invasive Bildgebung von Fluoreszenz in Kardiomyozyten im lebenden Tier ermöglicht. Dafür klonierte ich die Förster Resonance Energy Transfer (FRET) -Sensoren EPAC1-camps als cAMP-Sensor und cGi500 als cGMP-Sensor und injizierte diese in befruchtete Zebrafisch Embryonen. Anschließend benutzte ich die F0-Generation für Fluorescence Lifetime Imaging (FLIM) -FRET-Messungen von cAMP und cGMP. Da Ca2+ als wichtiger downstream Mediator von cAMP und cGMP die kardiale Kontraktion reguliert, klonierte ich außerdem den Ca2+-Sensor GCaMP6 und benutzte den Farbstoff Fluo-4 AM, um intrazelluläres Ca2+ darzustellen. Ergebnisse: Die klonierten Sensoren für cAMP, cGMP und Ca2+ konnten erfolgreich in den Zebrafisch injiziert werden und zeigten alle Expression in einzelnen Kardiomyozyten. Ich entwickelte ein Protokoll, dass die Fixierung von lebenden Zebrafisch Embryonen und nachfolgender Bildgebung von cAMP und cGMP mit hoher zellulärer Auflösung mit FLIM-FRET in vivo erlaubte. Ich konnte eine funktionelle Charakterisierung der Sensoren durchführen, indem ich zeigte, dass sie auf Konzentrationsänderungen von intrazellulärem cAMP und cGMP reagieren sowie zeigen, dass Zebrafische trotz fehlender T-Tubuli eine signifikante cAMP- und cGMP Kompartimentierung aufweisen, auch unter extremen Bedingungen nach Gabe von cAMP/cGMP stimulierenden Substanzen in hoher Dosierung. Ich konnte zudem subzelluläres Ca2+ durch konfokale Mikroskopie bildgebend darstellen und entwickelte ein Protokoll, um mit Fluo-4 AM eine schnelle Möglichkeit zu haben, Ca2+ mit in die Messungen einzubeziehen. Ausblick: Die in dieser Arbeit benutzte Methode bietet eine gute Möglichkeit, subzelluläre cAMP- und cGMP-Kompartimentierung und Ca2+ zu untersuchen und damit zum Beispiel die Fragen zu beantworten, ob eine veränderte cAMP/cGMP Kompartimentierung zu Herzkrankheiten wie Hypertrophie führt oder ob eine veränderte cAMP Kompartimentierung den zellulären Ca2+ Haushalt und damit die kardiale Kontraktion beeinflusst. Darüber hinaus kann das von mir etablierte Protokoll dazu genutzt werden, mehr über cAMP, cGMP und Ca2+ während der Regeneration im Herzen zu lernen, da der Zebrafisch über ausgeprägte Regenerationsfähigkeiten verfügt.

Tuberculosis continues to be one of the major causes of morbidity and mortality worldwide. Several mycobacterial species such as M. tuberculosis and M. africanum are responsible for causing this disease in humans. Reports of high cAMP levels in mycobacterial species (as compared to other bacteria such as E. coli) suggested that this second messenger may play an important role in the biology of mycobacteria. Further, it was reported that infection with mycobacteria led to an increase in the cAMP levels within the host macrophage. More recent studies have shown that this cAMP increase may be due to bacterially derived cAMP, hinting at a role for cAMP in mycobacterial pathogenesis. Given this background, the study of cAMP in mycobacteria proves to be an interesting field of research. Signalling through cAMP involves an interaction of this cyclic nucleotide with a cAMP-binding protein. These proteins typically contain a cyclic nucleotide-binding domain (CNB domain) linked to another (effector) domain. The CNB domain is thought to allosterically control the activity of the effector domain, thus mediating cellular responses to altered cAMP levels. For example, in the case of eukaryotic protein kinase A (PKA), binding of cAMP to the CNB domain results in relieving the inhibitory effects of the regulatory subunit on the catalytic subunit. The catalytic subunit then phosphorylates its target substrates, eliciting a variety of cellular responses. This work involves the characterisation of novel cAMP-binding proteins from mycobacteria, in an attempt to better understand cAMP signalling mechanisms in these organisms. The genome of M .tuberculosis H37Rv is predicted to code for ten CNB domain-containing proteins. One of these genes is Rv0998 (KATmt). KATmt was found to contain a GCN5 related N-acetyltransferase (GNAT) domain linked to a CNB domain. KATmt finds orthologues throughout the genus Mycobacterium, thereby suggesting its role in the basic physiology of these organisms. In addition, such a domain fusion is unique to mycobacteria and hence promises to deliver insights into the biology of this medically important genus. Presented here are the biochemical and functional characterisation of KATmt and its orthologue from M. smegmatis, MSMEG_5458 (KATms). Recombinant KATms bound cAMP with high affinity, validating the functionality of its CNB domain. Mutational and analogue-binding studies showed that the biochemical properties of the CNB domain were similar to mammalian protein kinase A and G-like CNB domains. The substrate for the GNAT acetyltransferase domain was identified to be a universal stress protein from M. smegmatis (MSMEG_4207). MSMEG_4207 was acetylated at a single lysine residue (Lys 104) by KATms in vitro. Further, cAMP binding to KATms increased the initial rate of acetylation of MSMEG_4207 by 2.5-fold, suggesting allosteric control of acetyltransferase activity by the CNB domain. To ascertain that KATms acetylated MEMEG_4207 in vivo, an in-frame deletion of the KATms gene was generated in M. smegmatis (ΔKATms). MSMEG_4207 was immunoprecipitated from wild-type M. smegmatis and the ΔKATms strains, followed by mass spectrometric analysis. Acetylated MSMEG_4207 was only present in the wild-type strain, confirming that KATms and MSMEG_4207 is an in vivo enzyme-substrate pair. Key biochemical differences were observed between KATms and KATmt. KATmt had an affinity for cAMP in the micromolar range, close to three log orders lower than that of KATms. In addition, KATmt showed strictly cAMP-dependent acetylation of MSMEG_4207. This demonstrates that orthologous proteins often evolve under varied selective pressures, resulting in divergent properties. Using a combination of bioluminescence resonance energy transfer (BRET) and amide hydrogen/deuterium exchange mass spectrometry (HDXMS), the conformational changes that occur upon cAMP binding to the CNB domain of KATms were monitored. A BRET-based conformation sensor was constructed for KATms by inserting KATms between GFP2 (green fluorescent protein) and Rluc (Renilla luciferase). An increase in BRET upon cAMP binding to the sensor was observed. HDXMS analysis revealed that besides the CNB domain, the only other region that showed conformational changes in KATms upon cAMP-binding was the linker region. To confirm that the linker region was important in propagating the effects of cAMP-binding to the acetyltransferase domain, an additional construct for BRET analysis encompassing the CNB domain and the linker region was generated. The magnitude of the increase in BRET was similar to the full length BRET-based sensor, validating the crucial role of the linker region in propagating cAMP-mediated conformational changes. A ‘PXXP’ motif found in the linker region, showed maximum exchange in HDXMS analysis. Mutation of both these proline residues to alanine in KATms, as well as KATmt, resulted in decoupling of cAMP-binding and allosteric potentiation of acetyltransferase activity. In contrast to the intricate parallel allosteric relays observed in other CNB domain-containing proteins, the CNB domain in KATms functions as a simpler cyclic nucleotide binding-induced switch involving stabilization of the CNB and linker domain alone. Therefore, KATms is an example of a primordial CNB domain where conformational changes are a consequence of binding-induced ordering alone. Using a computational approach, putative substrate proteins of KATmt from M. tuberculosis were identified. The substrate specificity of lysine acetyltransferases is determined loosely by a consensus sequence around the lysine residue which is acetylated. Using this property of protein acetyltransferases, the genome of M. tuberculosis H37Rv was mined for proteins harboring lysine residues in a similar sequence context as seen in MSMEG_4207. In vitro biochemical analysis of some of the predicted substrates helped confirm a subset of enzymes belonging to the fatty acyl CoA synthetase (FadD) class as substrates of KATmt. The acetylation of FadDs by KATmt was cAMP-dependent. In each of the four proteins tested, acetylation was found to occur at a single conserved lysine residue. To confirm that FadDs were acetylated by KATmt in vivo, BCG_1055, the orthologue of KATmt in M. bovis BCG, was deleted using the specialised transduction method. FadD13, one of the FadDs acetylated by KATmt in vitro, was immunoprecipitated from wild-type M. bovis and the ΔBCG_1055 strains using a FadD13-specific polyclonal antibody. Acetylated FadD13 was almost completely absent in ΔBCG_1055 but substantial amounts of acetylated FadD13 were present in the wild-type strain, indicating that FadD13 was indeed an in vivo substrate of KATmt. The functional consequences of acetylation of FadDs were analysed using an in vitro fatty acyl CoA synthetase assay. The activities of FadD2 and FadD13 were inhibited on acetylation with KATmt, while acetylation of FadD5 resulted in the formation of a novel product. Therefore, modification of the highly conserved lysine residue in these enzymes by acetylation led to loss or alteration of their enzymatic activity, suggesting that acetylation may be used as a regulatory mechanism to modulate the activities of some of the FadDs by KATmt in a cAMP-dependent manner. Given the extensive role of FadDs in cell wall biosynthesis and lipid degradation in mycobacteria, it seems possible that post-translational control by KATmt in a cAMP-dependent manner constitutes a novel mechanism utilised by these bacteria to regulate these pathways. This direct regulation of protein lysine acetylation by cAMP appears to be unique to mycobacteria, as orthologues of KATmt are not found outside this genus. In addition, the biochemical differences between KATmt and its orthologue from M. smegmatis KATms, indicate species specific variation, on a common theme. This study is the first report of protein lysine acetylation in mycobacteria. In addition to the identification of several proteins subject to this post-translational modification, the effect of acetylation on the enzymatic activities of some of them has been elucidated.