The work presented in this master thesis deal with the issue of quenching, investigation regarding different cooling rates and its effect on the material properties of aluminum alloy in the 6xxx series used for crash purposes in cars, such as crash boxes, beams and other crash relevant parts. Precipitation of Mg2Si due to different cooling rates affects the material properties such as crash performance, thus the aluminum alloy used is sensitive to different cooling rates. In order to perform tests with different cooling rates a cooling rig was constructed. In order to evaluate the different cooling rates both mechanical testing such as tensile test and 3-point bending test and compression test were performed. Also analyses with scanning electron microscope/energy-dispersive x-ray spectroscopy were performed to estimate grain boundary decoration of Mg2Si due to the different cooling rates. Furthermore LOM analyses were performed to evaluate if the experimental setup had any effect on material properties such as grain size. The constructed cooling rig produced different cooling rates with reliable repeatability as intended. Cooling rates between 130 ̊C/s and 20 ̊C/s were accomplished. Mg2Si occurred in all investigated test samples with various amounts. Higher cooling rates decreases the precipitation of Mg2Si to the grain boundaries, higher cooling rates also increased the bending angle achieved from the 3-point bending test. Furthermore, extensive solution heat treatment at elevated temperatures leads to grain growth.

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During the last 10 years, low pressure carburizing and high pressure gas quenching has become more popular since it is a “new” process and researchers reports that the distortion characteristics gained from these processes is improved in relation to conventional processes. The aim of this work was to investigate the distortions gained from three different heat treatment processes on main shaft gears and crown wheels. Experiments with atmospheric carburization with oil quenching and low pressure carburization with nitrogen gas quenching or oil quenching were made and distortion characteristics gained from these processes were then measured and compared. It was found that components treaded in the relatively new LPC-HPGQ process are similar and often even better than the conventional method. The larger main shaft gear improves the most with newer method but instead gets a lower core hardness and systematic diametrical shrinkage. Some measurements on the crown wheel were also improved but others were affected negatively.

Clostridium thermocellum is a thermophilic bacterium that converts biomass to ethanol directly; however, high sensitivity of this bacterium toward ethanol limits its commercial utility. To elucidate the effect of ethanol on the growth of this bacterium a metabolite analysis of C. thermocellum was performed. The hypothesis of the project was that exogenous ethanol alters the metabolite profile of C. thermocellum. For metabolite analysis, capillary electrophoresis-electrospray ionization-mass spectrometry method (CE-ESI-MS) was developed due to highly polar and charged nature of metabolites. To increase the sensitivity of CE-ESI-MS, several parameters at the ESI interface were optimized. The application of 50% isopropanol as a sheath liquid increased sensitivity for metabolite analysis dramatically. Trimethylamine acetate (pH 10) was used as background electrolyte (BGE) due to its ability to separate the structural isomers of glucose phosphate. For metabolite sample preparation, novel methods for quenching and CE compatible metabolite extraction protocols were developed. Newly developed protocols were applied to metabolite analysis of wild type (WT) and ethanol adapted (EA) strains of C. thermocellum grown in batch cultures. Significant differences were found in key intracellular metabolites such as NAD+ and pyruvic acid. Intracellular concentrations of NAD+ were low in EA cells compared to WT cells and pyruvic acid was only detected in EA cells. To further understand the effect of ethanol on metabolite fluxes, WT and EA cells were grown in increasing concentrations of ethanol and the metabolite profile for each ethanol treatment was obtained. Significant changes were found in intracellular metabolite concentrations. Metabolic data showed that the glycolysis process in WT cells was obstructed due to exogenous ethanol which was evident from accumulation of G6P. On the other hand, no such accumulation of G6P was observed in the EA strain; however pyruvate began to accumulate in EA strain. These changes in intracellular metabolite concentrations due to perturbation of exogenous ethanol supported the hypothesis. Also, this investigation revealed a correlation between ethanol and metabolite profile changes and was able to explain a possible mechanism of growth inhibition of C. thermocellum which will certainly help genetic engineers to develop superior strains of C. thermocellum for commercial cellulosic ethanol production.

<p>This thesis focuses on the numerical and theoretical studies of gas quenching in industrial furnaces. Gas quenching is the rapid cooling of metal pieces, aiming at forcing a phase transformation of the metal structure to improve its mechanical properties. The numerical methodology has been evaluated with respect to the desired accuracy and different aspects of the flow with importance for achieving an optimized process have been investigated. Initially, attention was paid to the flow and heat transfer fields both in an empty furnace and in a furnace loaded with different charges with the objective to study the influence of the charge configuration on the flow and heat transfer uniformity. This study led to the identification of several possible improvements, which are currently being implemented by the industrial partners of this project. As earlier studies had shown the importance of flow uniformity on the quality of the heat treatment, the subsequent work focused substantially on the flow uniformity upstream of the quenching zone resulting in design recommendations for the particular type of furnace under consideration. The dependence of the performance of the coolant medium on its composition was investigated theoretically and an analysis of most important parameters was carried out. Improved knowledge of the effect of gas mixture composition on heat transfer was added to the body of knowledge already available.</p>

Photocontrol, the use of light-sensitive proteins to control events within living tissue, allows complex processes in higher organisms to be studied. The Halorhodospira halophila photoactive yellow protein (PYP) can be used to regulate transcription factor activity with blue light. Before any PYP-based system can probe complex processes in higher organisms, proof of functional expression in vivo is required. We linked d25 PYP to the C-terminus of blue fluorescent protein (BFP) and expressed variants of the fusion protein (BFPd25PYP) in E. coli and human embryonic kidney (HEK293) cells. Expression of BFPd25PYP in E. coli verified in vitro photoswitching. The fusion protein was successfully expressed in HEK293. Fluorescence studies of intact cells indicated chromophore uptake and incorporation into PYP in HEK293, while photoswitching of PYP was measured in protein isolated from HEK293. These findings are promising for the development of applications using PYP for in vivo mammalian photocontrol of biological events.

Photocontrol, the use of light-sensitive proteins to control events within living tissue, allows complex processes in higher organisms to be studied. The Halorhodospira halophila photoactive yellow protein (PYP) can be used to regulate transcription factor activity with blue light. Before any PYP-based system can probe complex processes in higher organisms, proof of functional expression in vivo is required. We linked d25 PYP to the C-terminus of blue fluorescent protein (BFP) and expressed variants of the fusion protein (BFPd25PYP) in E. coli and human embryonic kidney (HEK293) cells. Expression of BFPd25PYP in E. coli verified in vitro photoswitching. The fusion protein was successfully expressed in HEK293. Fluorescence studies of intact cells indicated chromophore uptake and incorporation into PYP in HEK293, while photoswitching of PYP was measured in protein isolated from HEK293. These findings are promising for the development of applications using PYP for in vivo mammalian photocontrol of biological events.

Three different types of cage crown ethers have been prepared and their complexation properties with Ag(I) have been studied. Atomic absorption, fluorescence quenching, and UV absorption have been used to study the interaction between the hosts (cage crown ethers) and guests (Ag+). For the cage-annulated crown ethers that contain aromatic rings, cation-π and π-π interactions may contribute significantly to the overall complexation ability of the host system. Piperazine groups may cooperate, and the piperazine nitrogen atoms provide unshared electrons, which may form a complex with Ag+. In addition, relatively soft donor atoms (e.g., Br) are well-suited for complexation with Ag+, which is a softer Lewis acid than alkali metal cations.

Machine components made of steel are subjected to heat treatment processes for improving mechanical properties in order to enhance product life and is usually done by quenching. During quenching, heat is transferred rapidly from the hot metal component to the quenchant and that rapid temperature drop induces phase transformation in the metal component. As a result, quenching generates some residual stresses and deformations in the material. Therefore, to estimate the temperature distribution, residual stress, and deformation computationally; three-dimensional finite element models are developed for two different steel components – a spur gear and a circular tube by a static and a dynamic quenching analyses, respectively. The time-varying nodal temperature distributions in both models are observed and the critical regions are identified. The variations of stress and deformation after quenching along different pathways for both models are studied. The convergence for both models is checked and validations of the models are done.

Regarding the great importance of fast cooling in steel industry for the production processes, a deep understanding of heat transfer and fluid dynamics must be held. A steel plate is heated up until a maximum temperature of 700 ⁰ C to be the n cooled down seconds later by a configuration of multi ple impinging water jets. Different flow rates are used simultaneously by different adjacent jets to perform quenching over the sample, so different hardness is obtained in the material over a small area . Temperature drop in time i s measured and monitored by embedded thermocouples and LabVIEW program. To achieve greater understanding of the quenching performance with different flow rates , several parameters are selected to be varied in order to achieve the best working conditions. Jet diameter takes values between 4 and 10 mm, initial temperature of quenching varies from 400 to 700 ⁰ C , subcooling temperature is tested for 65 and 75 K, and jet velocity varies between 1.9 and 3.9 m/s. The result of total number of 9 expe riments shown that v ariation of jet diam eter does not influence substantially on the cooling rate if flow rate is kept constant. High initial quenching temperature (600 - 700 ⁰ C ) led to slightly higher cooling rate in the stagnation region of water jets. The peak value of heat transfer rate in the upwash flow zone was more highlighted for initial quenching temperature 600 ⁰ C and below it. High er values of subcooling and jet velocity produce better cooling rates. The result shown higher jet velocity at one column of water jets changes position of upw ash flow slightly toward the adjacent column of jets with lower jet velocity. In general, the result shown that all the studied parameters did not have negative effect on obtaining various cooling rates over the steel plate.