Despite over a century of inquiry, the mechanisms that achieve the close matching of oxygen supply to demand during exercise remain elusive. It has been proposed that in addition to its role as the primary oxygen carrier, the red blood cell (RBC) functions as a roving oxygen sensor, linking the oxygen demand at the muscle with oxygen delivery via the circulation (Ellsworth et al. 1995). It is hypothesised that the RBC would release adenosine triphosphate (ATP) in proportion to the number of unoccupied binding sites on the haemoglobin molecule as it traverses regions of high oxygen demand such as the microcirculation of active skeletal muscle. ATP would then stimulate the release of vasodilatory substances from the endothelium which would diffuse to neighbouring vascular smooth muscle resulting in vasodilation and an increase in blood flow in accordance with the oxygen demand set by the muscle. The first step in establishing a role for this mechanism during exercise in humans is to determine whether ATP increases in the venous blood draining an active muscle bed. Based on the handful of published studies, there is an increase in ATP concentration in the femoral vein during knee extensor exercise. However the response has not been studied in other vascular beds in humans. As such, the main aim of this thesis was to measure the ATP response to dynamic handgrip exercise. Secondary aims were to determine whether the response was modified by hypoxia, and to provide information about the timing of the changes in ATP concentration during a bout of handgrip exercise. These questions were addressed in Studies 3 and 4. Because blood flow is central to this hypothesis, a substantial portion of this thesis was also associated with the measurement of forearm blood flow (FBF) using venous occlusion strain gauge plethysmography (VOSGP), and this was conducted in Studies 1 and 2. VOSGP is based on the assumption that with venous outflow prevented, any increase in limb volume is proportional to the rate of arterial inflow. The rate of arterial inflow is determined as the slope of the change in limb volume over time. The slope must be calculated over the initial linear portion of this relationship, when arterial inflow is unaffected by the inevitable rise in venous pressure associated with venous occlusion. VOSGP was initially used to measure blood flow at rest and in response to pharmacological interventions which produced only modest increases in arterial inflow (Joyner et al. 2001). However, measurement of the high rates of arterial inflow that occur with exercise may challenge the limits of this technique. Tschakovsky et al. (1995) reported a marked reduction in arterial inflow over the first four cardiac cycles during venous occlusion following static handgrip exercise that elevated blood flow to 22-24 mL/min/100mL. Only during the first cardiac cycle was arterial inflow unaffected by cuff inflation. As such, the window for measuring high rates of arterial inflow may be very brief. Therefore Study 1 aimed to determine whether blood flow could be measured using VOSGP across the range of arterial inflows that occur with dynamic handgrip exercise. Participants (n = 7) completed four, five-minute bouts of dynamic handgrip exercise at 15, 30, 45, and 60% of maximum voluntary contraction (MVC). FBF was measured using VOSGP at rest, and following five minutes of dynamic handgrip exercise. The slope of the change in limb volume was measured over the first one, two, three, and four consecutive cardiac cycles following the onset of occlusion. FBF was 2.5 ± 0.5 at rest, and 16.5 ± 4.9, 24.9 ± 9.4, 44.1 ± 22.0, and 57.8 ± 14.9 mL/min/100mL following five minutes of exercise at 15, 30, 45, and 60% MVC, respectively. At rest, arterial inflow decreased across the four cardiac cycles (P = 0.017 for the main effect), however post-hoc pairwise comparisons revealed no significant differences between any of the cardiac cycles. In contrast, the inclusion of two, three, or four cardiac cycles at 30 and 60% MVC, and three or four cardiac cycles at 15 and 45% MVC resulted in reductions in calculated arterial inflow compared with using the first cardiac cycle alone (P > 0.05). The inclusion of just two cardiac cycles resulted in a 9-26% reduction in calculated arterial inflow depending on the workload. This reduction was even more pronounced when three (19-40%) or four (26-50%) cardiac cycles were included. In conclusion, resting FBF can be calculated over at least four cardiac cycles during venous occlusion at rest. However, exercising FBF should be calculated from the first cardiac cycle only following dynamic handgrip exercise across the range of intensities used in this study. This extends the findings of Tschakovsky et al. (1995) who demonstrated this effect following handgrip exercise at a single intensity. Study 2 was designed to establish the FBF response to dynamic handgrip exercise, whether the workloads produced different blood flow responses, and to establish the within- and between-day reproducibility of FBF measured using VOSGP. In Part A (within-day reproducibility), participants (n = 7) completed three trials of dynamic handgrip exercise at four intensities (15, 30, 45, and 60% MVC), with each exercise trial separated by 10 minutes of rest. In Part B (between-day reproducibility) participants (n = 7) completed three trials of dynamic handgrip exercise at 15, 30, and 45% MVC on three separate days within a two week period. FBF was measured at rest, and each minute of exercise during brief (5-7 second) pauses in contractions. FBF response. FBF increased from rest at all workloads (P > 0.05), and then plateaued between Minutes 1 to 5 at the 15 and 30% MVC workloads and between Minutes 2 and 5 at the 45% workload (P > 0.05 for each minute compared to Minute 5). Too few participants completed the 60% workload to permit any statistical analysis. FBF reached values of 13.0 ± 2.0, 26.8 ± 8.4, 44.8 ± 14.9, and 52.9 ± 5.1 mL/min/100mL in the final minute of exercise at the 15, 30, 45, and 60% MVC workloads. FBF was different between the 15, 30, and 45% workloads by Minute 3 (P > 0.05). Reproducibility. The within-day test-retest reliability of exercising FBF was poor to moderate (ICC = 0.375-0.624) with individual coefficients of variation (CVs) ranging from 6-25%, 9-23%, and 9-31% for the 15, 30, and 45% MVC workloads, respectively. The between-day test-retest reliability for resting FBF was moderate (ICC = 0.644, P > 0.05; individual CVs between 1 and 31%). Between-day test-retest reliability for exercising FBF was poor to moderate (ICC = 0.381-0.614), with individual CVs ranging from 14-24%, 8-23%, and 6-18% for the 15, 30, and 45% workloads, respectively. It was concluded from this study that VOSGP provides adequately reproducible measurements to detect changes in FBF of the magnitude seen between workloads in this study. However, the variability in the measurement precludes its use when smaller differences are of interest. Based on the previous findings reporting an increase in ATP concentration during dynamic knee extensor exercise in the leg (Gonzalez-Alonso et al. 2002; Yegutkin et al. 2007), Study 3 was designed to determine whether ATP concentration increased in the venous effluent during dynamic handgrip exercise in the forearm. Since the deoxygenation of haemoglobin is a primary stimulus for ATP release from red blood cells, a further aim was to determine whether this response was augmented by systemic hypoxia. Participants (n = 6) completed four, five-minute bouts of dynamic handgrip exercise at 30, 45, 65, and 85% MVC under normoxia (inspired oxygen fraction = 0.21) and hypoxia (inspired oxygen fraction = 0.12). Blood samples for the determination of ATP concentration were drawn at rest and 180 seconds after the onset of exercise at each workload from a catheter inserted into a forearm vein. Venous plasma ATP concentration at rest was 0.28 ± 0.11 μM/L and remained unchanged during exercise at workloads up to 85% MVC (P > 0.05). Systemic hypoxia, sufficient to reduce arterial oxygen saturation to 83 ± 2%, also failed to alter the plasma ATP concentration (P = 0.148). The lack of a change in ATP concentration was unexpected but there are several possible explanations. It is possible, although unlikely, that ATP was not released in the forearm microcirculation. The previous demonstration that ATP increased in response to static handgrip exercise (Forrester and Lind 1969) would suggest that this was probably not the case. When considered in the context of the findings from Study 4, the most plausible explanation is that a less than optimal blood sampling site may have hindered the measurement of a change in ATP. The blood flow response at the onset of dynamic exercise in the forearm is at least biphasic; Phase 1 describes the immediate, large increase in blood flow within 2 seconds of the onset of exercise and is believed to be governed by mechanical factors whereas Phase 2 has a latency of ~20 seconds and describes a further, slower increase until blood flow reaches steady state (Saunders et al. 2005b). The temporal characteristics of Phase 2, along with the fact that blood flow during this phase is closely related to the metabolic rate of the muscle, suggest regulation by metabolic factors. Currently there is scant evidence detailing the time course of vasodilator release, although it is important to demonstrate that the release of a vasodilatory substance precedes the blood flow response it is proposed to influence (Delp 1999). ATP is released from red blood cells in proportion to the offloading of oxygen and a reduction in the oxygen content of venous blood draining a muscle bed occurs within 10 seconds of the onset of exercise. Thus the release of ATP should follow soon thereafter. As such, Study 4 was designed to determine whether ATP increased in the venous effluent of the forearm following 30 and 180 seconds of dynamic handgrip exercise at 45% MVC; and whether this increase corresponded with a decrease in venous oxygen content. Participants (n = 10) completed two bouts of dynamic handgrip exercise at 45% MVC; the first was one minute in duration, and the second was four minutes in duration. Venous blood samples for the determination of ATP and venous oxygen content were drawn at rest and during exercise from a catheter inserted in a retrograde manner into the median cubital vein. Arterialised samples for the estimation of arterial blood gases and ATP concentration were obtained from the non-exercising hand. ATP concentration in arterialised blood from the non-exercising arm was 0.79 ± 0.30 μM/L at rest and remained unchanged at both time points during exercise (P > 0.05). ATP concentration in the venous blood of the exercising arm increased from 0.60 ± 0.17 μM/L at rest to 1.04 ± 0.33 μM/L 30 seconds after the onset of exercise (P > 0.05), and remained at this higher level after 180 seconds (0.92 ± 0.26 μM/L, P > 0.05 versus rest). This corresponded with a decrease in venous oxygen content from 103 ± 23 mL/L at rest to 68 ± 16 mL/L 30 seconds after the onset of exercise (P > 0.05) and 76 ± 15 mL/L (P > 0.05 versus rest) 180 seconds into exercise. Furthermore, at 180 seconds of exercise, ATP concentration was moderately and inversely related to venous oxygen content (r = -0.651, p > 0.05). In conclusion, this study provides the first evidence that ATP concentration is increased in the blood draining the exercising forearm muscles in response to dynamic handgrip exercise. The finding that ATP concentration was increased just 30 seconds after the onset of exercise is also novel, and particularly interesting in the context of the recently reported dynamic response characteristics of the forearm blood flow response. In conclusion, the work contained within this thesis provides several important findings. The first study has provided evidence that measuring high rates of arterial inflow using VOSGP is possible, but that the window for making these measurements is small, probably as brief as a single cardiac cycle. The second study demonstrated that while the reproducibility of forearm blood flow measurements using VOSGP is poor, it is adequate to detect the large changes that occurred between workloads. However, VOSGP cannot be used to detect more modest differences. Common to both Study 3 and 4 was the measurement of ATP at rest, and 180 seconds after the onset of dynamic handgrip exercise at 45% MVC. The primary difference was the position of the catheter which was inserted in an antegrade manner in Study 3, and in a retrograde manner in Study 4. Since ATP was unchanged in Study 3 but increased under similar conditions in Study 4, it is likely that ATP was also released during exercise in Study 3, but that a less than optimal blood sampling site precluded its measurement. This illustrates the necessity to sample blood from as close as possible to the probable site of ATP release, the muscle microcirculation. The most important and novel findings from this body of work come from Study 4. This is the first study to demonstrate an increase in ATP concentration in the forearm in response to dynamic handgrip exercise. However, the most novel finding was that ATP concentration was elevated just 30 seconds after the onset of exercise. Such an early increase has not previously been reported during dynamic exercise in any vascular bed. This is an important finding since establishing the time course for the release of vasodilatory substances is critical to our understanding of the mechanisms that regulate blood flow during exercise.

Made available in DSpace on 2014-06-11T19:28:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-07-13Bitstream added on 2014-06-13T19:58:20Z : No. of bitstreams: 1 castilho_aa_me_sjc.pdf: 568029 bytes, checksum: 0b5b31953733da6dcf83b1d44aeb677d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo neste estudo foi avaliar a alteração dimensional provocada pelos procedimentos de soldagem nas infra-estruturas de próteses fixas impanto-suportadas por meio de análise de extensometria linear elétrica assim como se determinar a resistência flexão de dois tipos de juntas soldadas (Arco-Plasma e Laser) em liga Odontológica Co-Cr. Para tanto, estruturas metálicas foram fundidas pelo método de indução para pilares tipo micro-unit. Para esse estudo, foram investigados três grupos: Grupo controle (G I: fundição em Monobloco, n=10), Grupo Arco-Plasma (G II: fundição segmentada e soldagem da estrutura em Arco-Plasma, n=10) e Grupo Laser (G III: fundição segmentada e soldagem da estrutura em LASER, n=10) as quais após assentamento em modelo de análise extensométrica, foram coletadas três leituras de microdeformação (με) para cada amostra. Os grupos, após ensaio de extensometria linear elétrica, foram submetidos ao teste de resistência à flexão (MPa) em máquina de ensaios universal (EMIC, 0,5 mm/min, 500Kg). As médias ±DP dos valores de microdeformação observados para as condições foram para o grupo Monobloco:461,9με; para o grupo Arco-Plasma: 637,0με e para o grupo Laser : 526,8με. Os resultados obtidos após o teste de resistência à flexão foram: para o grupo Monobloco: 1857,2MPa; para o grupo Arco-Plasma: 1820MPa e para o grupo Laser : 1068Mpa. Uma comparação em análise extensométrica entre os três grupos, quanto à distribuição dos valores de alteração dimensional, foi efetuada por meio do teste de Kruskal-Wallis. Verificou-se que a distribuição dos valores de alteração dimensional não difere Estatisticamente (estatística kw = 1,77; gl = 2; p = 0,414 > 0,05). Uma comparação entre os três grupos, quanto à distribuição dos valores... / The aim of this study was to evaluate the dimensional changes caused by the welding procedures in implant-supported prostheses infrastructure by strain gauge analysis as well as to determine the flexural strength values of two types of welded joints (Arc-Plasm and Laser) in Co-Cr dental alloy. Thus, metal structures were casted by induction method for micro-unit type abutment. For this study, three groups were evaluated: Control Group (G I: monoblock casting, n = 10), Arc-Plasm Group (G II: casting and welding of structures in Arc-Plasm, n = 10) and Laser Group (G III: casting and welding of structures in Laser, n = 10), in which were, after placing the samples in the strain gauge analysis model, three micro strain (με) measurements for each specimen were collected. The groups, after strain gauge analysis, were submitted to flexural strength (MPa) test in an universal testing machine (EMIC, 0.5 mm / min, 500Kg). Mean ± SD values of microstrain conditions observed for the Monoblock group were: 461.9 mε; for the Arc-Plasm group: 637.0 mε and the Laser group: 526.8 mε. The results, after flexural strength test, were: Monoblock group: 1857.2 MPa; Arc-Plasm group: 1820 MPa and Laser group: 1068 MPa. The strain gauge comparison analysis between the three groups, as well the distribution of dimensional changes values were made by Kruskal- Wallis test. It was found that the values´ distribution of dimensional changes do not differ statistically (KW statistic = 1.77, df = 2, p = 0.414> 0.05). A comparison between the three groups, in relation to the 108 distribution of the values of flexural strength, was performed by Kruskal- Wallis test. It was found that the values distribution of flexural strength differ statistically... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:28:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-03Bitstream added on 2014-06-13T18:58:12Z : No. of bitstreams: 1 abreu_cw_me_sjc.pdf: 508442 bytes, checksum: 720d525ea366cc2fd0575e67a37ba04e (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O objetivo desse estudo foi avaliar a aplicação de carga e distribuição das microdeformações ao redor de implantes com conexão protética cone morse. Três implantes foram inseridos num bloco de poliuretano e pilares microunit foram instalados nos implantes com torque de 20 Ncm. Três coifas usinadas foram adaptadas nos pilares para configurar a supraestrutura protética (n=5) e comparar com as supra-estruturas obtidas com coifas plásticas. Quatro extensômetros foram colados na superfície superior do bloco. Uma carga vertical estática de 30 Kg foi aplicada em cinco pontos das supra-estruturas. Os registros obtidos dos extensômetros foram submetidos ao teste estatístico ANOVA e Tukey (α=5%). As medidas derivadas dessa simulação revelaram que durante a aplicação de carga: (1) existiu uma relação direta do local de aplicação e na distribuição da microdeformação ao redor dos implantes; (2) não existiu diferença na microdeformação entre coifas plásticas e usinadas. / The aim of this study was to evaluate the load transfer and strain distribution by an implant-supported fixed partial prosthesis. Three implants Morse Taper were inserted in a polyurethane block and abutments microunit were installed on the implants with a torque of 20 Ncm. Plastic cylinders and prefabricated cylinders were adapted on abutments to fabricate the framework (n=5). Four strain gauges were attached on the superior surface of the block and then each framework was tight on the abutments with a torque of 10 Ncm and vertical load of 30Kg was applied to five points on the framework. The data obtained in the strain gauge analysis were submitted to the statistical tests ANOVA and Tukey (α=5%). The measurements derived from this simulation revealed that: (1) there was a direct proportion between the strain distribution in the metal framework and stresses created in the supporting structure around the implants; (2) there was not a strain difference between plastic and prefabricated cylinders during the load transfer

Made available in DSpace on 2014-06-11T19:35:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-07-07Bitstream added on 2014-06-13T20:25:48Z : No. of bitstreams: 1 kojima_an_dr_sjc.pdf: 500670 bytes, checksum: 06c2a32f2d0fe96b743e1f05b9e95270 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O objetivo deste estudo foi avaliar, por meio da extensometria, a distribuição de tensões ao redor de implantes em função dos diferentes locais para carregamento, tipo de coifa (plástica ou usinada) e configuração (linear e compensada). Para tanto, em um bloco de poliuretano, foram posicionados paralelos entre si e com uma configuração linear, implantes auto-rosqueáveis de hexágono externo, com dimensões de 3,75 X 13mm, a uma distancia de 7mm, de centro a centro. Em um outro bloco a fixação do meio foi deslocada em 2mm para gerar uma configuração compensada (offset). Pilares protéticos Micro-unit, com 3mm de cinta foram instalados sobre as fixações. Com duas matrizes em aço inoxidável foram confeccionados 10 enceramentos para cada bloco, distribuídos da seguinte forma: 5 para coifa plástica e 5 para coifas usinadas (n=5). A seguir esses padrões foram fundidos com uma liga de Co-Cr. Quatro extensômetros (strain gauges) foram colados na superfície superior de cada bloco tangenciando a plataforma de cada fixação. Um carregamento de 30kg durante 10s foi feito em cinco posições (A, B, C, D, E), sendo repetido três vezes para aquisição dos dados (em με) pela aparelho condicionador de sinais. Os dados obtidos foram submetidos a análise de variância (ANOVA) e teste de Tukey (p<0,05). Como conclusão da análise dos resultados obtidos pudemos observar que não houve diferença na utilização de coifas plásticas e usinadas, que não houve diferença entre as configurações linear e compensada; havendo diferença estatisticamente significante para os locais de carregamento / The aim of this study was to evaluate, using strain gauge, the load distribution surround the implants according differents loading sites, coping types (plastic and machined) and configuration (linear and offset). In one polyurethane block, three cylindrical implants with external hexagon (3.75mm x 13mm) were fixed parallel with 7mm between their centers in a linear configuration. In another block the midle implant was positioned in a 2mm offset. Micro-units abutments with 3mm of metallic neck were fixed. With two metallics matrix, it was manufactured 10 wax patterns for each block, distributed in the following form: 5 for plastic copings and 5 for machined copings (n=5). After, patterns were casted in cobalt-chromium alloy. Four strain gauges were positioned on the upper surface of each polyurethane model around the implants. An axial load of 30kg within 10 seconds in five positions (A, B, C, D, E), with three repetead measurements for data aquisition (in με) by the multichanel bridge machine. The datas were submitted to ANOVA and Tukey test (p<0.05). Such as conclusion from the obtained data treatment: there was no difference between the plastic and machined copings, there was no difference between the linear and offset configuration; there was statiscal significance only with the differents loading sites.

Orientador: Fernando Eidi Takahashi / Banca: Estevão Tomomitsu Kimpara / Banca: Alfredo Mikail Melo Mesquita / Resumo: O objetivo neste estudo foi avaliar a alteração dimensional provocada pelos procedimentos de soldagem nas infra-estruturas de próteses fixas impanto-suportadas por meio de análise de extensometria linear elétrica assim como se determinar a resistência flexão de dois tipos de juntas soldadas (Arco-Plasma e Laser) em liga Odontológica Co-Cr. Para tanto, estruturas metálicas foram fundidas pelo método de indução para pilares tipo micro-unit. Para esse estudo, foram investigados três grupos: Grupo controle (G I: fundição em Monobloco, n=10), Grupo Arco-Plasma (G II: fundição segmentada e soldagem da estrutura em Arco-Plasma, n=10) e Grupo Laser (G III: fundição segmentada e soldagem da estrutura em LASER, n=10) as quais após assentamento em modelo de análise extensométrica, foram coletadas três leituras de microdeformação (με) para cada amostra. Os grupos, após ensaio de extensometria linear elétrica, foram submetidos ao teste de resistência à flexão (MPa) em máquina de ensaios universal (EMIC, 0,5 mm/min, 500Kg). As médias ±DP dos valores de microdeformação observados para as condições foram para o grupo Monobloco:461,9με; para o grupo Arco-Plasma: 637,0με e para o grupo Laser : 526,8με. Os resultados obtidos após o teste de resistência à flexão foram: para o grupo Monobloco: 1857,2MPa; para o grupo Arco-Plasma: 1820MPa e para o grupo Laser : 1068Mpa. Uma comparação em análise extensométrica entre os três grupos, quanto à distribuição dos valores de alteração dimensional, foi efetuada por meio do teste de Kruskal-Wallis. Verificou-se que a distribuição dos valores de alteração dimensional não difere Estatisticamente (estatística kw = 1,77; gl = 2; p = 0,414 > 0,05). Uma comparação entre os três grupos, quanto à distribuição dos valores... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this study was to evaluate the dimensional changes caused by the welding procedures in implant-supported prostheses infrastructure by strain gauge analysis as well as to determine the flexural strength values of two types of welded joints (Arc-Plasm and Laser) in Co-Cr dental alloy. Thus, metal structures were casted by induction method for micro-unit type abutment. For this study, three groups were evaluated: Control Group (G I: monoblock casting, n = 10), Arc-Plasm Group (G II: casting and welding of structures in Arc-Plasm, n = 10) and Laser Group (G III: casting and welding of structures in Laser, n = 10), in which were, after placing the samples in the strain gauge analysis model, three micro strain (με) measurements for each specimen were collected. The groups, after strain gauge analysis, were submitted to flexural strength (MPa) test in an universal testing machine (EMIC, 0.5 mm / min, 500Kg). Mean ± SD values of microstrain conditions observed for the Monoblock group were: 461.9 mε; for the Arc-Plasm group: 637.0 mε and the Laser group: 526.8 mε. The results, after flexural strength test, were: Monoblock group: 1857.2 MPa; Arc-Plasm group: 1820 MPa and Laser group: 1068 MPa. The strain gauge comparison analysis between the three groups, as well the distribution of dimensional changes values were made by Kruskal- Wallis test. It was found that the values' distribution of dimensional changes do not differ statistically (KW statistic = 1.77, df = 2, p = 0.414> 0.05). A comparison between the three groups, in relation to the 108 distribution of the values of flexural strength, was performed by Kruskal- Wallis test. It was found that the values distribution of flexural strength differ statistically... (Complete abstract click electronic access below) / Mestre

O implante dental depende do osso de suporte para sua estabilidade e ongevidade funcional. O comportamento mecânico e a estrutura trabecular do osso afetam a interface com o implante. Tal conhecimento pode fornecer uma base para melhoramentos na função e desenho dos implantes atuais minimizando a reabsorção da crista óssea e falha na junção osso-implante. Desta forma a correta quantificação das forças que atuam sobre o sistema de implante e principalmente no osso de suporte ao qual ele está inserido é importante para o entendimento da biomecânica possibilitando que intervenções possam ser realizadas pelos profissionais visando assim à longevidade das próteses implanto-suportadas bem como dos implantes que as sustentam. Por essa razão, a proposta deste trabalho foi avaliar, com auxílio de extensômetros elétricos, se o processo laboratorial de confecção de coroas metalocerâmicas resulta em alterações das tensões no osso ao redor dos implantes. Para isto foi utilizado um modelo de poliuretano em forma de U simulando o osso mandibular com dois implantes de hexágono externo de 3,75mm de diâmetro por 13 mm de comprimento, nos quais foram fixados intermediários multi-unit. Os corpos de prova foram confeccionados com dois tipos de ligas odontológicas - Cobalto-Cromo e Paládio-Prata - e três tipos de cilindros protéticos calcinável, usinado de Cobalto-Cromo e usinado de Ouro. As leituras de deformação foram realizadas em dois momentos: 1- após a fundição das infra-estruturas e 2 após estas infra-estruturas terem recebido cobertura cerâmica. Os testes foram realizados parafusando os corpos de prova sobre os intermediários utilizando-se para isto um dispositivo eletrônico de torque com força máxima de 10 Ncm. Os resultados mostraram que, após a aplicação da cobertura cerâmica, ocorreu piora da condição de assentamento aos intermediários. O mesmo não foi detectado quando se avaliou a deformação do osso simulado, já que os valores de deformação não ndicaram diferenças antes e após a aplicação da cerâmica. / The dental implant depends on the supporting bone for its stability and functional longevity. Mechanical behavior and trabecular bone structure affect the interface with the implant. Such knowledge may provide a basis for improvements in the current implant function and design, decreasing bone crest resorption and failure at bone-implant junction. Adequate quantification of forces that act upon the implant system and mainly on the supporting bone is important for understanding the biomechanics, allowing the intervention by professionals with the purpose of achieving the longevity of implant-supported prostheses. Therefore, this work aims at evaluating, with the aid of strain gauges, whether the laboratorial process of metal ceramic crown fabrication results in changes of the tensions around the implants. A U-shaped polyurethane model simulating the mandibular bone with two 3.75-mm diameter and 13-mm length external hexagon implants were used with fixed multi-unit abutments. Specimens were prepared with two types of dental alloys - cobalt-chromium and silver-palladium - and three types of prosthetic cylinder castable plastic, prefabricated in cobalt-chromium or gold. The deformation readings were performed in two moments: 1- after framework casting and 2- after ceramic layering of the framework. The tests were performed with the framework screwed onto the abutments at 10Ncm using an electronic torque device. The results showed that the ceramic layering worsened the fit between the crowns and the abutments. The same was not detected when bone deformation was evaluated since the values were not statistically different.

A reabilitação protética de pacientes desdentados é uma área da Odontologia amplamente estudada por pesquisadores do mundo todo, e com o desenvolvimento dos implantes osseointegrados surgiram muitas perguntas sobre o desempenho das próteses instaladas sobre estes. Conhecer o comportamento mecânico do conjunto coroa unitária, intermediário, implante e tecido de suporte é importante para entender o processo de transmissão de forças e suas consequências, processos estes que ainda não estão totalmente compreendidos. O objetivo deste trabalho foi verificar a deformação gerada no intermediário e no osso peri-implantar após aplicação de uma carga inclinada sobre coroas unitárias metalocerâmicas. Foi utilizado um modelo mestre de poliuretano, simulando o osso mandibular, com um implante hexágono externo de 3,75mm x13 mm de comprimento, no qual foi fixado um intermediário multi-unit. O trabalho foi realizado com 3 grupos de 4 coroas cada, divididos de acordo com o tipo de cilindro e liga de fundição (cilindros de ouro sobrefundidos com liga de PdAg, cilindro de acrílico fundido com liga de NiCoCr e cilindros de CoCr usinado sobrefundidos com liga de NiCoCr). Cada corpo de prova foi submetido cinco vezes à aplicação de carga oblíqua de 15 e 30 graus no centro da oclusal de cada coroa com uma força de 300N em uma máquina universal de ensaios. Foram realizadas leituras das deformações geradas no intermediário e no poliuretano, com o uso de extensômetros lineares elétricos (strain gauges) os quais foram colados em três faces do intermediário (Disto lingual, Mesio lingual, Vestibular) e nas superfícies mesial, vestibular, distal e lingual do osso simulado ao redor do implante. Os dados obtidos foram submetidos à análise de variância a 3 critérios (ANOVA) e o teste de Tukey, utilizando um nível de significância (p<0,05). Os resultados mostraram que entre as duas angulações utilizadas existiram diferenças estatísticas significantes. Os valores médios de deformação se mantiveram dentro dos padrões de normalidade, na janela fisiológica descrita por Frost entre 50 &#x3BC;&#x3B5; e 1500 &#x3BC;&#x3B5; independente do tipo de cilindro utilizado, com exceção dos valores de compressão obtidos na carga oblíqua de 30 graus onde nos três tipos de cilindro ultrapassaram a janela de sobrecarga patológica com mais de 4000 &#x3BC;&#x3B5. / The prosthetic rehabilitation of edentulous patients is a dental area widely studied by worldwide researchers, and through the development of dental implants, appear many questions about the performance of prostheses installed on them. Knowledge of the mechanical behavior of the integration between single crown, abutment, implant and support tissue is very important to understand the forces transmission process and its consequences but these processes are not fully understood. The objective of the present study was to evaluate the deformation generated in the abutment and simulated peri-implant bone after application of an oblique load on metal-ceramic crowns. A polyurethane master model was used to simulate the jawbone with an implant external hexagon 3.75 mm x13 mm length, which was fixed in a multi-unit abutment. The study was conducted with twelve metal ceramic crowns divided according to type of cylinder and casting alloy, Group 1: Gold cylinder cast with PdAg alloy; Group 2: Acrylic cylinder cast with NiCoCr alloy; Group 3: CoCr cylinder cast with NiCoCr alloy, thus forming three groups of four sample each. Each specimen was subjected five times the application of oblique load of 15 and 30 degrees in the center of the occlusal of each crown with a 300N force in a universal testing machine. Readings were taken of the deformations generated in the abutment and bone simulated by polyurethane, with the use of electric linear strain gauges which have been positioned on three sides equidistant to each other to measure microstrains at abutment (disto-lingual, meso-lingual, Vestibular) and the simulated bone around each implant received four strain gauges, positioned on the mesial, distal, buccal and lingual aspects. The data were submitted to analysis of variance to 3 criteria (ANOVA) and Tukey test, using a significance level (p <0.05). The results showed that between the two angles tested there were significant statistical differences. The deformation average values remained within normal limits, at the adapted state described by Frost between 50 &#x3BC;&#x3B5 and 1500 &#x3BC;&#x3B5 independent of the type of cylinder used, with the exception of compression values in oblique load of 30 degrees where the three types of cylinder surpassed the pathological overhead state with over 4,000 &#x3BC;&#x3B5.

Ce travail de th`ese est consacré à la conception, la réalisation technologique et la caractérisation fonctionnelle d'un nouveau type de capteur de champ magnétique MEMS 3D. Différent de l'approche classique utilisée pour des magnétomètres MEMS 3D, le capteur conçu dans le cadre de cette thèse n'a pas comme base le principe de la force de Lorentz, mais se sert des avantages qu'offrent les matériaux magnétiques intégrés dans des systèmes MEMS. Le matériau magnétique subit un moment lorsqu'il est soumis à un champ magnétique environnant. Le principe de détection du signal est basé sur la piézorésistivité et utilise des jauges en silicium mono-crystallin avec une section nanométrique. Le concept technologique convient également pour la fabrication de capteurs inertiels et est donc une approche prometteuse pour la fabrication des centrales de mesure inertielle (IMUs). La conception est principalement basée sur un modèle de bruit. En dehors de la considération des limitations technologiques, des aspects mécaniques, magnétiques et thermiques sont également pris en compte. Deux pistes ont été étudiées pour l'intégration du matériau magnétique. Une première option consiste dans l'intégration d'aimants terres-rares comme SmCo et NdFeB. Une deuxième option a pour objet l'intégration des multi-couches antiferromagnétiques et ferromagnétiques, couplées par le couplage d'échange. La réalisation technologique bien exigeante des ces deux approches sera présentée avec une concentration particulière sur les propriétés magnétiques des matériaux utilisés. Une autre partie sera consacrée à la caractérisation des contraintes mécaniques dans des couches minces qui peuvent devenir problématiques pour les micro-systèmes conçus dans le cadre de ce travail. Au final, la fabrication du capteur ainsi que des caractérisations fondamentales seront présentées afin d'établir une preuve expérimentale pour le concept du capteur. / This PhD thesis deals with the design, the technological implementation, and functional characterizations of a new type of monolothic 3D MEMS magnetometer. Other than for the classical approach used for 3D MEMS magnetometers, the sensor developed in this work is not based on the principle of Lorentz force, but takes advantage of magnetic material which is integrated into the MEMS device and experiences a torque when surrounded by a magnetic field. Signal detection is based on piezoresistive detection using gauges of monocrystalline silicon with nanometric section. The technological concept is also suitable for the fabrication of inertial sensors and thus a very promising approach for fabrication of inertial measurement units (IMUs). Sensor design mainly relies on a noise model. Besides technological limitations, mechanical, magnetic and thermal aspects are also taken into account. Two different methods for integration of magnetic material were explored. A first option consists in the integration of rare-earth magnets like SmCo and NdFeB, a second option is about the integration of exchange-bias coupled antiferromagnetic and ferromagnetic multilayers. The technologically challenging implentation of both approaches will be discussed with a special focus on magnetic properties of the used materials. A further part will be dedicated to the characterization of mechanical stress in thin layers, which may become problematic for the microsystems conceived in this work. Finally, sensor fabrication and fundamental characterizations will be presented as experimental proof of concept for the sensor.

Geólogo / En el rubro de la minería siempre están muy presentes dos factores, la rentabilidad del negocio y la seguridad de los trabajadores. Para cumplir con ambas es necesario tener un acabado conocimiento sobre el comportamiento local de la roca. Entre las pruebas más básicas e importantes dentro de la caracterización física de la roca está el de compresión uniaxial no confinado (UCS), que como bien dice su nombre, es una prueba donde se aplica compresión en un solo sentido (vertical) a una muestra de roca cilíndrica, hasta que esta falle. Externamente se usa un dispositivo para medir la deformación, como los strain gauges y LVDT. Si bien estos son métodos confiables y ampliamente usados, en este trabajo, el objetivo es validar una técnica relativamente nueva en esta área, la medición de deformación usando correlación de imágenes digitales (DIC). El concepto básico de esta consiste en tomar una gran cantidad de fotos de un objeto en una ventana de tiempo en la que presente algún tipo de movimiento o deformación. Estas imágenes son luego procesadas en un software especializado que identifica los desplazamientos a través de la comparación de imágenes. Lo que se hace en este trabajo es realizar y grabar 15 ensayos UCS de carga fija en probetas de roca, con los que se busca determinar hasta donde llegan las capacidades del DIC en estudios de roca. En las pruebas realizadas se encuentra que los gráficos de deformación versus estrés son parecidos a los que se hacen con otros métodos. La cantidad de deformación es similar, mientras que la forma y eventos capturados en la curva dependen mucho del criterio de posicionamiento de las mediciones por DIC. Se confeccionan representaciones visuales de la deformación, donde se muestra, usando mapas de colores, las zonas que sufren desplazamiento durante el proceso de falla. Por último, en el análisis estructural de la secuencia se hace un detalle de cómo va apareciendo cada fractura durante la carga, dando la posibilidad de encontrar relaciones entre cada falla. A partir de los resultados obtenidos se concluye que el DIC es un método muy flexible en cuanto que puede hacer infinitas mediciones de la cara estudiada, de alta precisión y que entrega mucha más información que otros métodos. Todo esto hace que sea muy útil en tres escenarios: cuando se quieren hacer mediciones de deformación sobre una muestra, cuando se quiere mostrar visualmente la deformación ocurrida en la muestra y para hacer análisis estructurales muy enfocados.

Vid träning av baksida lår kan det vara svårt att förstå hur muskeln aktiveras. Genom att visualisera de myoelektriska signalerna från biceps femoris och semimembranosus till den som tränar kan personen få en bättre mind-muscle connection. I examensarbetet har två teoretiska EMG sensorer skapats och simulerats, kod har skrivits för att filtrera fyra EMG signaler samt överföra dessa över BLE, kraftsensorer är kopplade och kod är skriven för att avgöra den kraftutveckling som sker i en kontraktion av baksida lår. EMG sensorerna har jämförts med SparkFuns MyoWear muskelsensor, OpenBCI Cyton board och BioNomadix BN-EMG2-T. Båda de teoretiska lösningarna anses likvärdiga med ett billigare alternativ till Cryton Board, en flerkanalig lösning till MyoWear, BN-EMG2-T är för dyr att realistiskt implementeras till examensarbetets syfte. Simuleringarna visar att kretsarna behandlar signalen enligt tänkt sätt men det gick inte att bygga en prototyp då en pandemi har begränsat KTH:s verksamhet / One difficulty when training hamstrings is the understanding of how the muscle is activated. Through visualization of the myoelectrical signals from biceps femoris and semimembranosus to the exerciser, a better mind-muscle connection can be achieved. In this bachelor thesis, two theoretical EMG sensors were created and simulated, code to filter four EMG signals and transmit them through BLE was written, also a way to calculate how much force is applied in a hamstring curl was constructed. Both EMG sensors have been compared against SparkFuns MyoWear muscle sensor, OpenBCI Cyton Board and BioNOmadix BN-EMG2-T. The theoretical EMG sensors are interconvertible to a cheaper Cyton Board, a multichannel alternative to MyoWear, the BN-EMG2-T is too expensive to be a realistic alternative for this bachelor thesis attended purpose. Simulations show that the EMG sensors behave as intended but because of a pandemic, a prototype could not be created.