Connexins are transmembrane proteins that form hemichannels allowing the exchange of molecules between the extracellular space and the cell interior. Two hemichannels from adjacent cells dock and form a continuous gap junction pore, thereby permitting direct intercellular communication. Connexin 36 (Cx36), expressed primarily in neurons, is involved in the synchronous activity of neurons and may play a role in aberrant synchronous firing, as seen in seizures. To understand the reciprocal interactions between Cx36 and seizure-like neural activity, we examined three questions: a) does Cx36 deficiency affect seizure susceptibility, b) does seizure-like activity affect Cx36 expression patterns, and c) does acute blockade of Cx36 conductance increase seizure susceptibility. We utilize the zebrafish pentylenetetrazol (PTZ; a GABA(A) receptor antagonist) induced seizure model, taking advantage of the compact size and optical translucency of the larval zebrafish brain to assess how PTZ affects brain-wide neuronal activity and Cx36 protein expression. We exposed wild-type and genetic Cx36-deficient (cx35.5-/-) zebrafish larvae to PTZ and subsequently mapped neuronal activity across the whole brain, using phosphorylated extracellular-signal-regulated kinase (pERK) as a proxy for neuronal activity. We found that cx35.5-/- fish exhibited region-specific susceptibility and resistance to PTZ-induced hyperactivity compared to wild-type controls, suggesting that genetic Cx36 deficiency may affect seizure susceptibility in a region-specific manner. Regions that showed increased PTZ sensitivity include the dorsal telencephalon, which is implicated in human epilepsy, and the lateral hypothalamus, which has been underexplored. We also found that PTZ-induced neuronal hyperactivity resulted in a rapid reduction of Cx36 protein levels within. 30 minutes and one-hour exposure to 20 mM PTZ significantly reduced the expression of Cx36. This Cx36 reduction persists after one-hour of recovery but recovered after 3-6 hours. This acute downregulation of Cx36 by PTZ is likely maladaptive, as acute pharmacological blockade of Cx36 by mefloquine results in increased susceptibility to PTZ-induced neuronal hyperactivity. Together, these results demonstrate a reciprocal relationship between Cx36 and seizure-associated neuronal hyperactivity: Cx36 deficiency contributes region-specific susceptibility to neuronal hyperactivity, while neuronal hyperactivity-induced downregulation of Cx36 may increase the risk of future epileptic events. / Doctor of Philosophy / Within the brain, cells (neurons) communicate with each other to pass along information. This communication is important for normal functions of the brain such as learning and memory, muscle movement, etc. Epilepsy is a disease of the brain that is caused by rapid over synchronized communication between cells. This leads to seizures which can include convulsions, loss of attention, and much more. Currently, 30% of patients suffering from epilepsy do not have a treatment option that works for them, it is, therefore, imperative to investigate new targets for treatment in this disease. Connexin36 is a protein in the brain that directly connects cells so they can pass information quickly between them. Connexin36, therefore, might make a good target for treatment. Previous work has aimed to understand this relationship but has been limited in their ability to look at the entire brain at any one time. The goal of this study was to understand the relationship between connexin 36 and brain hyperactivity across the whole brain simultaneously. To understand this relationship, we first determined what happened to brain activity if the protein was missing entirely after exposure to a seizure causing drug. We were asking: How does connexin 36 affect hyperactivity. We found that different regions of the brain responded differently without the connexin 36 protein. This suggests that one size does not fit all, and one must look at the whole brain to understand the effects of the connexin 36 protein. Next, we asked a similar question, but in the opposite direction, how does hyperactivity affect connexin 36? We found, in the short-term, hyperactivity reduced the amount of connexin 36 present in certain regions of the brain. This continued until 3 hours following exposure to the seizure causing drug Pentylenetetrazol (PTZ). Lastly, to determine if this short-term reduction in connexin 36 meant that an individual was more likely to experience hyperactivity. To do this, we used a connexin 36 blocking drug, then introduced the seizure causing drug at different concentrations. We found, at all concentrations, the connexin 36 blocking drug caused significant changes in neuronal activity, depending on the brain regions. Overall, our results showed that connexin 36 plays an important role in hyperactivity and that a short-term reduction in connexin 36 is detrimental, and may contribute to an increase in the possibility of subsequent hyperactivity.

This thesis examines the state of media literacy in the middle school curriculum of Virginia's public schools. Through in-depth interviews with state certified teachers of English and Language Arts, the goal was to uncover student, teacher, resource, family, classroom, school, and other structural variables that influence media literacy among students at the middle school level, while also uncovering teachers' perception of the Standards of Learning (SOLs) and the benchmarks for media literacy that are contained within those state directives. An additional purpose of this thesis is to contribute to theory building efforts so that media literacy education is better understood in academic literature, in higher education, and in K-12 curriculum. / Master of Arts

No / The intention of this paper is to promote the model of knowledge-based collaborative supply chain management (KBCSCM) system as an alternative strategy for organisations to resolve the problems in their current supply chain management (SCM) in the era of collaborative commerce (c-commerce).

No / This paper presents issues associated with the needs of collaborative supply chain management (CSCM) and proposes a planning stage of a CSCM framework. The proposed planning stage of a CSCM framework incorporates issues of organisation profile, internal functional strategy and supplier-customer strategy. The gauging absence of prerequisites (GAP) analysis technique which embedded in the knowledge-based system is proposed in the planning stage to analyse the gap between the current and the desirable position (benchmark) for an effective implementation in organisation. The planning stage framework provides information specifically for designing a CSCM by focusing on the organisation capability and business processes and discussed the important issues in planning a CSCM for business organisations, specifically for a manufacturing environment. Further research could be carried out to capitalise the framework for improving the CSCM. Practical implications ¿ The proposed planning stage of a CSCM framework enables the chain members to identify key factors or issues for CSCM development.

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Syfte Syftet är att undersöka de brister entreprenörer upplevt efter att ha använt sig av Crowdfunding och om Internetbaserade plattformar underlättar informationsasymmetrin mellan finansiär och entreprenör. Samt om entrprenörer anser att Crowdfunding har potential att fungera som självstående finansieringskälla. Teori Tidigare studier visar att finansieringsformen Crowdfunding ofta är bristfällig. Få entreprenörer får tillräckligt kapital och finansieringsformen saknar kompetens och resurser. Metod Kvantitativ enkätundersökning under perioden 11 november 2015 till och med 25 november 2015. Resultat Resultatet från enkätundersökningen bestående av 40 deltagare presenteras i diagram. Analys I analysen jämförs tidigare studier och teorier med resultat och letar samband och kopplingar som förklarar resultatet. Respondenterna ser potential i Crowdfunding. De flesta upplever att de fått tillräckligt kapital och är positivt inställda till skillnad mot tidigare teorier. Slutsats Slutsatsen i undersökningen blir att en del Crowdfuninganvändare använder en annan finansieringskälla också där eget kapital och banklån är vanligast. Tekniken och dålig exponering var de problem entreprenörer ansåg Crowdfundingplattformarna ha, dock har de flesta tro på att Crowdfunding har potential att fungera som en själv-stående finansieringsform.

Digital divide research has recently begun to address the functional gaps between Internet-connected technologies, specifically mobile and wired devices. This study uses nationally representative survey data from the Pew Internet and American Life Project to address this area of research and explores how smartphone-dependence compared to multi-modal access impacts Internet use among key demographic groups including race, sex, age, income, and education. This study also explores how demographic characteristics and smartphone use interact to affect reliance on smartphones and perceptions of the utility of mobile devices. Results show that race, sex, age, income, and education, exhibit different rates of smartphone-dependence, and also perform different online activities with their smartphones. Minorities and younger users are more likely to be smartphone-dependent and multi-modal users suggesting that these demographic groups are adopting mobile Internet technologies faster than Whites and older individuals. Minorities also use smartphones for more news and information activities than Whites, which contradicts traditional usage gap predictions.

Ledarskap har visat sig vara en utav de viktigaste faktorerna för att främja hälsa i arbetslivet. Tidigare forskning betonar att ett hälsofrämjande ledarskap där chefen bland annat är engagerad i de anställdas arbete och tillåter inflytande i beslutstagande ökar medarbetarnas trivsel och välbefinnande i arbetet, samt kan minska risken för sjukskrivning. Det är dock inte alltid chefen når ut med alla delar av sitt ledarskap, vilket kan resultera i ett ”gap” mellan chefens och medarbetarnas uppfattning. Medarbetarna kan då bli omotiverade i arbetet, vilket påverkar såväl individens välbefinnande som företagets och samhällets ekonomi. Syftet med denna studie var att utifrån begreppet hälsofrämjande ledarskap undersöka hur första linjens chefer på ett kommunalt helägt aktiebolag inom energisektorn uppfattade sitt eget ledarskap i jämförelse med medarbetarnas uppfattning av ledarskapet, samt om detta relaterade till medarbetarnas välbefinnande i arbetet. Denna studie är en fördjupning av ett tidigare projekt, GodA och är baserad på tvärsnitts design där både kvantitativa och kvalitativa metoder användes. För att jämföra chefens och medarbetarnas uppfattning av ledarskap användes ett urval av enkätfrågor från GodA. Chefens värdering av sitt ledaskap utifrån hälsofrämjande aspekter undersöktes genom semistrukturerade intervjuer. Resultatet visade att det fanns en bred uppfattning kring innebörden av ett hälsofrämjande ledarskap hos cheferna. De upplevde även en viss svårighet i rollen som första linjens chef. Jämförelsen mellan chefens och medarbetarnas uppfattning av ledarskapet resulterade i ett gap, då hälften av grupperna hade en signifikant skillnad. Det fanns även en positiv signifikant korrelation mellan gapet och medarbetarnas välbefinnande, där en större skillnad resulterade i sämre välbefinnande. Då tidigare forskning visat att chefer ofta överskattar sitt ledarskap och att ett gap kan påverka medarbetarnas välbefinnande, är det viktigt att vidare undersöka vad skillnaden kan bero på och hur gapet kan minskas. Det är även relevant att skapa en tydlig definition av ett hälsofrämjande ledarskap i syfte att utveckla hälsofrämjande strategier i arbetet. / Aim: Based on the concept of health promotion leadership, the purpose is to examine how the first-line managers perceive their own leadership in comparison to employees' perceptions of the leadership, and whether this relates to employee well-being at work. Methods: This study is a recess of the project GodA and it’s based on a cross-sectional design with both quantitative and qualitative methods. To make a comparison between managers and employees perception of leadership, a selection of survey questions from GodA was used. The manager’s valuation of the health promotive leadership aspects were examined through semi-structured interviews. Main results: The results shows that there was a wide perception of health promotive leadership among managers. Although it can be interpreted that the managers work from the health promoting leadership aspects, there was a gap between the manager and employee perception of leadership. In half of the groups there was a significant difference. There was also a positive significant correlation between gap and employee well-being, then a larger gap resulted in a lower estimated well-being. Summary: Previous research shows that managers often overrate their leadership and a gap can affect employee well-being. It is therefore important to further investigate what the differences between the manager and the employees can depend on and how the gap can be reduced. It is also important to create a clear definition of a health promotive leadership in order to develop health promotion strategies at work.

Analysis of the National Sample Survey Data from 2011-2012 shows that a gender-based education gap exists. Women are more likely than men to be illiterate. Some parents continue to view household duties as more important than education in the case of girls, causing some to drop out in primary and middle school, which leads to lower experience accumulation. However, females are almost equally as likely as males to be enrolled in school, and an equal proportion of males and females earn higher education degrees. More importantly, the difference in resource allocation seems to be minimal. Although education has a strong, positive impact on wages, returns to education for women are lower than those for men. This is taken into account by parents when making education decisions for their children. As a result, the wage gap appears to be a cause and effect of the education gap.

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