A reflection of the lighting artist’s approach for the lighting design of The Twenty-fifth Annual Putnam County Spelling Bee. University of Massachusetts, Amherst Department of Theater’s Fall 2010 production used as a research ground for the experimentation of lighting design through the cueing process.

Microbiomes are an extremely vast and complex network of microorganisms. Bacteriophages are a key factor in the microbial health of an ecological system and impact the evolution of pathogenic bacterial strains. Bacteria and the phages that infect them have an intricate relationship due to the dependency on the bacterial host for phage replication, the ability of the phage to lyse and kill its host, and the horizontal gene transfer between the host and phage. This thesis aimed to understand how bacteria and the bacteriophages that infect them impact an ecological system, with a focus on disease states. By analyzing all bacteriophages targeting a specific host, genomic properties, physical similarities and differences a better understanding of how a group of tailed phages have evolved numerous mechanisms and tools to infect host bacteria was understood. The microbiome study of the Western honey bee Apis mellifera, comparing the microbial communities of colonies infested with the external parasite Varroa destructor against those not infested revealed a need for more directed treatment of Varroa infestations. Through our study we discovered that the honey bee microbiome is much more complex than previously reported, consisting of hundreds of bacterial species. In addition, through comparing infested and healthy colonies, we discovered that infestation of Varroa destructor mites negatively impact the colony microbiome in part by reducing microbes key in digestion and immune health of honey bees. Results in this thesis indicate that two microbes which have not been previously established as part of the key microbes in honey bee guts, Xenorhabdus and Sodalis, may in fact be key to honey bee health as they were both effected negatively by the presence of Varroa mite infestations. These bacteria have been shown to be involved in immune health in other insects, supporting this hypothesis. The final stage of this thesis involved the development of an acaricide bacteriophage therapy designed to target key microbes in the gut of V. destructor. Our therapy was shown to safely treat honey bee colonies infested with this parasite. It can be concluded that while phages are a normal part of the microbial ecosystem of the intestinal tract of organisms, altering that balance by increasing the phage load on the microbiome to target specific beneficial microbes drastically reduces the overall fitness of the organism. Results from this study indicate that multi-target cocktail and single-target phage therapy are an effective low impact biological Varroacide. The discovery of an effective therapy is important and to better understand the results of this thesis, further investigation is required, including a study of the impact of the phage therapy on the mite microbiome, as well as the safety of the therapy to humans.

Accepted theories of honey bee foraging state that foragers are active at only 1 time of day. It has been shown that a few foragers can be trained to forage at multiple times of day and at many locations. The purpose of the current study was to further investigate the phenomenon of foragers maintaining multiple time-memories. It was found that in small and large sample populations, a minority of foragers could be trained to 2 or more times and places. Within the hive, the foragers that do not fly to the stations also tend to exhibit a persistent time-memory. Remaining experienced foragers cluster at the dance floor at the approach of a training time and remain dispersed throughout the hive at other times. Because foragers can only be recruited from the dance floor, these foragers that stay behind are also exhibiting a time-memory with respect to the proper training time.

Throughout the 20th century, a significant amount of work was conducted at archaeological sites in Utah Valley dating to the Archaic, Fremont, and Late Prehistoric periods. Despite the amount of work conducted, very little has actually been reported in the past. As a result, very little has previously been known about either the prehistoric archaeology of the Provo River Delta area or the historic archaeological work that has taken place. This thesis presents a synthesis of historical work, a reanalysis of artifacts from previous excavations, and a review of extant documentation (including field notes, maps, and student reports) of several sites in the Provo River Delta, including the Hinckley Mounds, Seamons Mound, and the Bee Sites.

Ralph Waldo Emerson’s bee poem, “The Humble-Bee,” expresses the nineteenth-century transcendentalist philosophy of finding wholeness and oneness in nature while Sylvia Plath’s twentieth-century bee poems function as a response to Plath’s feelings of alienation and repression, indicating that transcendental peace is lost in the postmodern era. Emerson’s poem indicates the spiritual fulfilment found through observing bees and highlights the harmony between humans and nature, but women of the nineteenth century find difficulty achieving this same level of freedom; Emily Dickinson reclaims the language of transcendentalism in her bee poetry to explore a world otherwise denied to her. The effects of the industrial revolution then sparked a mass disconnect between humans and nature, a disconnect reflected in the bee poetry of Sylvia Plath; she rejects the inherited tradition of transcendentalist poetry by using her bee poems to demonstrate discomfort within nature and society.

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The honey bee (Apis mellifera) is important both ecologically, as a generalist pollinator, and economically by pollinating our crops and producing honey. Honey bees use plants for foraging for pollen and nectar, which together constitute their entire diet. Yet, as flowering times of plants vary during the summer, so do the floral resources available. Honey bees are known to be selective for their food sources. Thus, their specificity in plant choices could vary according to the availability of flower sources, showing a differing usage of plant diversity in different timepoints. Alternatively, the honey bees selectivity to fulfill their nutrient needs could lead to a constant usage of diversity. The taxonomic origin of the plant DNA found in honey can be identified and used to investigate the plant taxa the bees have collected nectar and pollen from. This study asks whether the diversity of plants which honey bees use varies during summer (June, July, and August). I used two diversity indices as response variables: 1) the Shannon-Wiener diversity index and 2) the number of plant genera identified in the DNA in the honey. I used data gathered from 41 hives from 14 Finnish beekeepers. An ANOVA test revealed no significant difference among the three timepoints in either response variable, indicating honey bees select a constant diversity of plants throughout the season. The result suggest that different plants can fulfill honey bees’ requirements at different parts of the summer. Honey bees are generalists, but selective, producing a relatively constant usage of plants throughout the season.

The small hive beetle (Aethina tumida) is an invasive pest from sub-Saharan Africa that has posed increasing threats to European honey bee (Apis mellifera) colonies in the United States over the past two decades. While control has been attempted, consistently effective management strategies still not been developed. This study sought to explore novel experimental methods to better understand and use A. tumida biology to target this pest. One aspect of A. tumida biology that has emerged as potential basis for improved control is olfactory manipulation, which could be used to disrupt beetles as they seek out A. mellifera colonies. Through olfactometry and electroantennography, key volatiles in A. tumida attraction and repulsion were tested and sensitivity of A. tumida to several attractants and repellents was quantified on behavioral and physiological levels. An additional source of attractive volatiles is the A. tumida fungal symbiont Kodamaea ohmeri, which ferments larval waste and is present throughout the A. tumida lifecycle, both externally and in the GI tract. This study explored the development of feeding and soil bioassays to test the effects of several insecticides on A. tumida larvae. Feeding and injection bioassays were also used to deliver a fungicide with the goal of repressing K. ohmeri, which was expected to detrimentally impact A. tumida health. The results of this work enhance our current knowledge or A. tumida biology and provide a useful basis for development of safe and selective management A. tumida management options for the future. / Doctor of Philosophy / The small hive beetle is an invasive European honey bee pest that poses a significant threat to apiaries in the United States. These beetles feed on hive products and brood, pollute the hive with fermenting waste, and, in severe infestations, cause colonies to abandon their hives. This project investigated previously unexplored control options that take small hive beetle biology into account. Small hive beetles have an exceptional sense of smell compared to other beetles, and this ability helps them to locate honey bee hives. Therefore, behavioral responses to attractants and repellants were tested through olfactometry, in which beetles were given a choice to travel toward or away from specific odors or odor blends. Responses to these odors on a physiological level were also quantified through electrical recordings of beetle antennae. Small hive beetles are also known to have a yeast-like symbiont, which is present throughout the small hive beetle lifecycle, both externally and internally. Feeding bioassasy for small hive beetle larvae, along with soil bioassays for the delivery of insecticides were also developed and used to test several compounds against small hive beetle larvae. Feeding bioassays were also used to deliver a fungicide to larval and adult beetles with the goal of repressing internal fungal activity The results of these studies help expand the knowledge of small hive beetle olfaction and provide a background for the development of novel control options to effectively manage this destructive pest.

Honey bees are economically important pollinators of a wide variety of crops that have attracted the attention of both researchers and the public alike due to unusual declines in the numbers of managed colonies in some parts of the world. Viral infections are thought to be a significant factor contributing to these declines, along with exposure to agricultural and apicultural pesticides, but viruses have proven a challenging pathogen to study in a bee model and interactions between viruses and the bee antiviral immune response remain poorly understood. Recent studies have demonstrated an important role for inwardly-rectifying ATP-sensitive potassium (KATP) channels in the cardiac regulation of the fruit fly antiviral immune response, but no information is available on their role in the heart-specific regulation of bee immunity. The results of this work demonstrate that KATP channel modulators have an observable effect on honey bee heart rate that supports their expected physiological role in bee cardiac function. Here, it is also reported that the entomopathogenic flock house virus (FHV) infects adult bees, causing rapid onset of mortality and accumulation of viral RNA. Furthermore, infection-mediated mortality can be altered by pre-exposure to KATP channel modulators. Finally, this work shows that exposure to environmental stressors such as commonly used in-hive acaricides can impact bee cardiac physiology and tolerance to viral infection. These results suggest that KATP channels provide a significant link between cellular metabolism and the antiviral immune response in bees and highlight the significant impact of environmental stressors on pollinator health. / Ph. D.