Doctor of Philosophy / Department of Biology / John M. Blair / Approximately 96% of native tallgrass prairie in North America has been lost, which accentuates the need for effective methods to restore the structure and function of these degraded ecosystems. Many prairie restorations aim to restore grass and forb species in proportions reflecting plant species diversity in native prairie. A target grass-forb species mixture is typically chosen at the onset of restoration, but often, grasses become excessively dominant and forbs are underrepresented as the community develops. Several studies have examined the potential for increasing forb cover and diversity in newly restored grasslands, but few studies have assessed factors limiting forb cover and diversity in well-established grass-dominated prairie restorations. The primary objective of this research was to assess the potential for enhancing plant species diversity and productivity in an established grass-dominated prairie restoration by selective removals of dominant grass species, and by manipulating resources (soil nutrients, light availability) or mycorrhizal interactions. A 7-year old grass-dominated restoration was used to evaluate plant and soil responses to manipulations in three separate studies. The first study examined the potential suppressive effects of dominant grasses on plant diversity by reducing the cover and biomass of two dominant grass species, Andropogon gerardii and Panicum virgatum. After 3 years, the removal of A. gerardii increased species richness and diversity, which was correlated with increased light availability, but not changes in soil resources. The second study examined the responses of restored grassland communities to long-term manipulation of soil resources (nutrient availability or soil depth), and to aboveground biomass removal via mowing. The long-term manipulation of soil resources did not alter plant species diversity, but nitrogen and light availability were important factors regulating plant productivity. The third study assessed the effects of manipulating arbuscular mycorrhizal (AM) fungi, through the use of either commercial inoculum or fungicide, on plant communities in restored prairie. Mycorrhizal suppression reduced grass productivity, suggesting that fungicide may be useful for enhancing diversity of restored prairies that are dominated by obligate mycotrophic grasses. In total, these studies suggest that competition between dominant grasses and subordinate forbs limits plant diversity in restored tallgrass prairie.

Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal. / Une petite population endémique, peu étudiée, occupe maintenant les eaux de l'estuaire moyen du Saint-Laurent et du fjord du Saguenay. Des données sur la densité, la composition (âges et sexes) des hardes, l'alimentation et les déplacements en août 1973, juin-septembre 1974 et juin-août 1975 sont tirées de l 200 heures d'observations systématiques (85,5% d une falaise haute de 15 m a l'embouchure du Fjord), et d'interviews avec environ 250 pêcheurs et ex-chasseurs de bélugas. De la falaise, on a observé 2 379 bélugas (max. 255/jour, le 22 juin 1975). On a suivi un troupeau de 20-25 animaux pendant 15,5 heures le ler août 1975. La densité mensuelle moyenne variait de 0,6 a 9,3 individus/h, sauf en septembre 1974 (25,1 indiv./h); la densité minimale a semblé se produire en juillet (0,6/h en 1974, 2,6/h en 1975). Les bélugas occupent le Saguenay de la mi-juin jusqu'en novembre; on les retrouve principalement dans les anses les plus importantes du Fjord inférieur, .dont sept aires présumées d'alimentation (probablement surtout du Capelan) a des profondeurs de 0,75-50 m. On a reconnu 88 individus avec marques naturelles, l un identifie plusieurs fois en trois ans et un autre en deux ans dans le Fjord inférieur. A l embouchure du Fjord, les bélugas nagent ordinairement vers l'amont durant l'étale de mer basse et vers l'aval durant la phase rapide du reflux. Ils suivent des trajets habituels en montant ou descendant le Saguenay, souvent près du rivage ou les courants de marée leur seraient favorables. Le troupeau suivi pendant 15,5 heures a passé 32,8% du temps dans trois aires présumées d'alimentation hors du Fjord et 67,2% a se déplacer entre de telles aires. Les bélugas ont parcouru 58 km à une vitesse moyenne de 3,74 km/h. Si la population de bélugas du Saint-Laurent est de 500 individus, alors leurs besoins métaboliques seraient de 18,34 tonnes métriques de nourriture (Capelan) par jour. / A small, little studied, endemic population of bélugas, or white whales, now occupies the Middle St. Lawrence Estuary and Saguenay Fjord. Data on density, sex and age composition of herds, feeding and movements in August 1973, June-September 1974 and June-August 1975 were obtained through more than 1 200 hours of systematic visual observations (85,5% from a 15 m cliff at the mouth of the Fjord), and interviews with about 250 fishermen and ex-beluga whalers. From the cliff, 2 379 bélugas (max. 255/day, June 22, 1975) were observed. A school of 20-25 bélugas was followed during 15,5 hours on August 1, 1975. Mean monthly density varied from 0,6 to 9,3 individuals/h, except in September 1974 (25,1 indiv./h); minimum density seems to occur in July (0,6/h in 1974, 2,6/h in 1975). Bélugas occupy the Saguenay from mid-June until November, and occur mainly in the more important coves of the lower Fjord, seven of which were presumably feeding areas (probably mostly for Capelin) at depths of 0,75-50 m. A total of 88 individuals with natural markings were recognized, one being seen several times in three years and another in two years in the lower Fjord. At the mouth of the Fjord, bélugas usually travel upstream during slack low water and downstream at fast ebb. They follow habitual routes on their way up or down the Saguenay, often close to shore where these tidal currents are presumably more favorable. The school followed during 15,5 hours spent 32,8Z of the time in three presumed feeding areas outside the Fjord and 67,2% travelling between such areas. They swam over 58l<m at a mean speed of 3,74 km/h. If there are 500 bélugas in the St. Lawrence population, they will have a metabolic need for 18,34 metric tons/day of food (capelin).

Master of Science / Department of Biology / Jesse B. Nippert / The Konza Prairie in northern Kansas, USA contains over 550 vascular plant species; of which, few have been closely studied. These species are adapted to environmental stress as imposed by variable temperature, precipitation, fire, and grazing. Understanding which plant traits relate to drought responses will allow us to both predict drought tolerance and potential future shifts in plant community composition from changes in local climate. Morphological and physiological measurements were taken on 121 species of herbaceous tallgrass prairie plants grown from seed in a growth chamber. Gas exchange measurements including maximum photosynthetic rate, stomatal conductance to water vapor, and intercellular CO[subscript]2 concentration were measured. All plants were exposed to a drought treatment and were monitored daily until stomatal conductance was zero. At this point, critical leaf water potential (Ψ[subscript]crit), an indicator of physiological drought tolerance was assessed. Other measurements include root length, diameter, volume, and mass, leaf area, leaf tissue density, root tissue density, and root to shoot ratio. Traits were compared using pair-wise bivariate analysis and principal component analysis (PCA). A dichotomy was found between dry-adapted plants with thin, dense leaves and roots, high leaf angle, and highly negative Ψ[subscript]crit and hydrophiles which have the opposite profile. A second axis offers more separation based on high photosynthetic rate, high conductance rate, and leaf angle, but fails to provide a distinction between C[subscript]3 and C[subscript]4 species. When tested independently, grasses and forbs both showed drought tolerance strategies similar to the primary analysis. Matching up these axes with long term abundance data suggests that species with drought tolerance traits have increased abundance on Konza, especially in upland habitats. However, traits that relate to drought tolerance mirror relationships with nutrient stress, confounding separation of low water versus low nutrient strategies. My results not only illustrate the utility of morphological and physiological plant traits in classifying drought responses across a range of species, but as functional traits in predicting both drought tolerance in individual species and relative abundance across environmental gradients of water availability.

Master of Science / Department of Biology / John M. Blair / Climate change predictions for the Great Plains region of North America include increased temperatures, changes to annual precipitation, and reduced growing season precipitation, which will likely alter grassland soil systems. To date, few studies have examined belowground community responses to predicted climate change scenarios, with fewer assessing long-term changes. My research focused on the impacts of long-term changes in precipitation and associated soil water content on belowground grassland systems (belowground plant biomass, soil carbon (C) and nitrogen (N) pools, microbial biomass C and N, and invertebrate communities) using recently collected samples from a long-term (16-yr) reciprocal core transplant between Konza Prairie Biological Station (MAP = 850 mm) and Kansas State Agricultural Research Center at Hays (MAP = 580 mm), with the Hays site having a long-term average annual precipitation amount that is ~30% less than the Konza site. Results from the experiment indicate that either increases or decreases in annual precipitation can have profound effects on belowground grassland systems. Belowground plant biomass, microbial biomass, and potential C mineralization rates were greater at the wetter Konza site regardless of soil origin. Total C stored in soils incubated at Konza was significantly greater as well, likely due to greater root inputs. The effects of precipitation were most apparent in the surface soil layers (0-20 cm), while soil origin impacted soil properties to a greater extent with increasing depth. This contrasted with results for the soil mesofauna, where total microarthropods responded negatively and nematodes responded positively to increased annual precipitation. Results of this study indicate important changes in soil C and N pools, belowground plant biomass, and soil mesofauna within grassland systems subject to changing precipitation regimes, and suggest more mesic prairie systems are more sensitive to changes in soil water availability than those in more arid grassland systems.

Doctor of Philosophy / Department of Biology / Brett K. Sandercock / Samantha Wisely / Anthropogenic activities and climate change have dramatically altered landscapes worldwide. The ability of species to cope and adapt to ongoing changes is likely a function of their behavior, movements, and sensitivity to fragmentation. Greater Prairie-Chickens (GPC) are a lek mating grouse native to the Great Plains Landscape Conservation Cooperative (GPLCC), for which inbreeding depression and anthropogenic avoidance are a concern. The goals of my dissertation were to: 1) identify genetic correlates of male performance which may influence population viability under current land use practices, 2) identify GPC habitat characteristics and delineate areas of critical GPC habitat necessary for GPC conservation, and 3) identify the relative importance of distance and habitat quality for maintaining genetic connectivity among spatially structured populations. First, I found male reproductive success and survival to be positively associated with genetic diversity. Using multistate modeling in Program Mark, male survival across the observed range of variation in number of alleles (15-22) increased more than fourfold from 0.17 to 0.77. Second, I found 35-40% of Kansas, and 1.5 % (11,000 Km squared) of the GPLCC, were considered high-quality lek habitats. Top performing logistic models predicting lek presence (wi=0.95) included strong effects of grassland cover and avoidance of anthropogenic disturbance. When this model was applied to putative future landscapes based on climate change and current land use trends over a 70-year period, I found a 27-40% reduction in habitat area and a 137 Km southeast shift in habitat distribution. Under equilibrium conditions we expect isolation by distance (IBD) to explain the distribution of genetic diversity. However, if the landscape restricts dispersal, then we might observe isolation by resistance (IBR). I used model selection procedures to choose among competing IBR or IBD models to explain the distribution of genetic diversity among GPC populations across Kansas and the GPLCC. IBD was never supported (R-square<0.02, P>0.09). The best models for Kansas (R2=0.69, P<0.02) and for the GPLCC (R-square=0.46, P<0.02) indicated that human-mediated landscape changes have influenced landscape permeability for dispersal. The integration of behavioral, landscape, and genetic data provided new insights on prairie-chicken ecology, and is a powerful approach for developing conservation strategies for sensitive species.

Master of Science / Department of Biology / Jesse B. Nippert / Jesse B. Nippert / Anthropogenic climate change is likely to alter the function and composition of ecosystems worldwide through increased precipitation variability and temperatures. To predict ecosystem responses, a greater understanding of the physiological and growth responses of plants is required. Dominant species drive ecosystem responses, and it is essential to understand how they respond to understand potential ecosystem changes. Dominant species, such as switchgrass (Panicum virgatum L.), posses large genotypic and phenotypic variability, which will impact the degree of responses to projected climate changes. I studied the physiological and growth responses of switchgrass, a common perennial warm-season C4 grass that is native to the tallgrass prairie, to alterations in precipitation amount and temperature. The first experiment I conducted focused on the responses of three ecotypes of P. virgatum to three precipitation regimes (average, 25% below, 25% above). I concluded that the physiological responses of photosynthesis, stomatal conductance, transpiration, dark-adapted fluorescence, and mid-day water potential in P. virgatum were explained by ecotypic differences. Robust responses to altered precipitation were seen in the water use efficiency, mid-day water potential, and aboveground biomass. Ecotypic differences were also seen in several aboveground biomass variables, and most strikingly in flowering times and rates. There were few interactions between ecotype and precipitation, suggesting precipitation is a strong driver of biomass production, whereas adaption of ecotypes to their local environment affects physiological processes. A second experiment studied the response of local populations of P. virgatum to nocturnal warming. Results showed significant differences in daytime E, daytime gs, and flowering phenology between treatments. Differences in aboveground biomass were between topographic positions. I concluded that water availability, based on topographic position, is a strong driver of P. virgatum aboveground biomass production, but nocturnal warming has the potential to impact flowering phenology, physiological responses, and exacerbate plant water stress. I also reviewed the literature on the ecological effects of implementing switchgrass cultivation for biofuel. From the literature review, I concluded that large-scale switchgrass cultivation will have widespread ecological impacts. If landscape heterogeneity is maintained through harvest rotations, no till farming, and mixed species composition, ecosystem services can be maintained while providing economic value.

Doctor of Philosophy / Department of Family Studies and Human Services / Sandra Stith and Jared Durtschi / Controversy still exists in whether parents should or should not use corporal punishment to discipline their young children. The aim of this study was to investigate whether corporal punishment when the child was two years old predicted child externalizing behaviors a year later, and whether or not this association was moderated by parents’ observed positivity and harshness towards their child. A total of 218 couples and their first born child were selected for this study from the Family Transition Project (FTP) data set. Findings indicated that frequency of fathers’ corporal punishment when the child was two years old predicted child externalizing behaviors a year later, while controlling for initial levels of child externalizing behavior. Also, it was found that observed positive parenting and observed harsh parenting moderated the relationship between corporal punishment and child externalizing behaviors. These results highlight the importance of continuing to examine the efficacy of a commonly used form of discipline (i.e., corporal punishment). Furthermore, this study suggests that the parental climate in which corporal punishment is used may also be important to consider because parental positivity and harshness attenuate and amplify, respectively, the association of corporal punishment with child externalizing. Implications for family therapy are offered.

Master of Landscape Architecture / Department of Landscape Architecture,Regional and Community Planning / Blake Belanger / Resilience theory provides an approach for landscape architects to analyze systems and design adaptive environments. C.S. Holling created the theory in response to changing social-ecological systems (Holling 1973). Resilience is the ability of a system to adapt to disturbances and remain in the same state (Walker and Salt 2006). This report proposes a framework that applies resilience to site analysis. The goal of the Resilience Analysis Framework is to help designers address expected and unexpected threats to human well being on a global and local scale. The framework was created by synthesizing findings from a literature review and expert interviews. A literature review based the framework in theory. Interviews with professionals working on the Rebuild by Design (2013) competition grounded the framework in professional practice. The goal of the Rebuild by Design competition was to develop resilient solutions to the changing environment. Synthesizing findings from the literature review and expert interviews resulted in a five part framework. The five parts are: Stakeholder Engagement, System Description & Goal Establishment, System Analysis, System Report, and Prioritization. Stakeholder Engagement is a process that occurs throughout each part of the framework. It includes education, data collection, reporting, and feedback. The System Description & Goal Establishment part describes the basic properties of a system and establishes goals for the future of those properties. System Analysis is an in depth evaluation of the factors determining a system’s level of resilience. The System Report synthesizes the important information from the System Description & Goal Establishment and System Analysis parts. Prioritization performs the essential task of focusing a project by identifying high priority systems. The goals (from the System Description & Goal Establishment and System Analysis parts) for the high priority systems determine the primary goals for the project. These goals inform decisions during the site analysis/strategic planning phase of the design process. The framework was applied to Washington Square Park in Kansas City, Missouri. This application provided an example of how to apply the framework to a park analysis. This report’s main finding was a framework for building evidence to make resilient design decisions.

Master of Science / Division of Biology / John M. Blair / Temperate grasslands are among the most threatened biomes in the world, with the largest historical losses due to conversion to agricultural land. While much of this biome has already been converted, there is concern the last remaining remnants in North America will be converted in response to increasing demand for crops used for ethanol production. Thus, restoring grasslands post-anthropogenic disturbance is increasingly important for conserving grassland biodiversity. Two major challenges for prairie restorations are establishing the many subdominant and rarer species found in native prairie, and offsetting the typical decline in richness and diversity over time as restorations age. Repeated seed addition of targeted species is commonly used to override low and declining plant richness and diversity. While this is generally effective early in restoration (i.e., as communities are establishing), its effectiveness in later stages (i.e., when established communities are often losing diversity) remains unknown. I investigated plant community responses to combinations of resource manipulations and disturbances coupled with a seed addition in a 15-yr old restored grassland to test the hypothesis that spatial resource heterogeneity increases the rate of colonization into established prairie restoration communities. Seeds were added to a long-term restoration experiment involving soil depth manipulations (deep, shallow) crossed with nutrient manipulations (reduced N, ambient N, enriched N). Seedling emergence was generally low and only 8 of the 14 forb species added were detected in the first growing season. I found no effect of increased resource heterogeneity on the abundance or richness of seedlings. There was a significant nutrient effect (p<0.1, α=0.1) on seedling abundance, with higher emergence in the enriched N than the ambient N treatment. I also found unexpected nutrient effects on richness, diversity and Mean C (Mean C = Σ CoCi*Ai, where CoC=Coefficient of Conservatism and A=relative abundance of the ith species). All values, except Mean C, were higher in the enriched N treatment than in either the reduced or ambient N treatments. Mean C was lowest in the enriched N treatment, and highest in the whole-plot control, suggesting that the majority of species contributing to higher richness and diversity in the enriched N treatment were “weedier” species. In a separate experiment, I found no effect of small-scale disturbances (aboveground biomass removal or soil disturbance) on seedling abundance or seedling richness. I did find a marginal effect of disturbance type on seedling richness (p=0.11, α=0.1), with higher seedling richness in the soil disturbance than the aboveground biomass removal treatment. I did not find any disturbance effects on community response variables. These results indicate that recruitment from seed additions into well-established restored communities is relatively low in the first year following a seed addition, regardless of resource availability and heterogeneity. Follow-up studies to determine recruitment rates in subsequent years are needed to elucidate whether recruitment responses are driven more by individual species differences or by environmental mechanisms.

Doctor of Philosophy / Department of Biology / Jack F. Cully, Jr. / Dispersal remains one of the most important, yet least understood, life history traits. As the vehicle of gene flow among populations, dispersal can both relieve inbreeding depression and prevent local adaptation. Regionally, dispersal can stabilize or destabilize metapopulations, given its critical roles in disease transmission among populations as well as recolonization following local extinction events. Furthermore, in light of climate change and increasing habitat loss and fragmentation, the ability to navigate through unfamiliar, unsuitable habitat between populations is essential to the long-term survival of a species across its range. In my dissertation, I present a multi-scale investigation of factors affecting gene flow and disease transmission among populations of a keystone species and an agricultural pest of the North American prairie: the black-tailed prairie dog (Cynomys ludovicianus). Black-tailed prairie dogs are social, ground-dwelling squirrels that live in spatially isolated populations called colonies. First, we conducted a landscape genetic analysis of black-tailed prairie dogs throughout a large portion of their current range. Our estimates of gene flow indicate that the genetic neighborhood size of both male and female prairie dogs reaches 40-60 km within short-grass prairie, whereas colonies within mixed-grass prairie are more isolated. At a broad scale, we observed isolation-by-distance among colonies and great influence of grassland productivity on genetic connectivity; however, neither distance nor landscape characteristics greatly explained observed genetic differentiation among colonies separated by < 50 km. Last, we investigated whether landscape features could predict disease transmission patterns of sylvatic plague among colonies in short-grass prairie and found evidence that pastures act as corridors for plague transmission. Our results indicate that black-tailed prairie dogs are more resilient to habitat loss and fragmentation than other obligate grassland species and likely capable of transmitting sylvatic plague over long distances. Taken together, these studies illustrate how a multi-scale approach can reveal complexities of dispersal dynamics that would otherwise remain undetected.