In Saccharomyces cerevisiae, RTT107 (ESC4, YHR154W) encodes a BRCT-domain protein that is important for recovery from DNA damage during S phase. I have found that Rtt107 forms a complex with the Slx1/Slx4 structure-specific nuclease. Deletion of SLX4 confers many of the same phenotypes observed in rtt107∆, including DNA damage sensitivity, prolonged DNA damage checkpoint activation, and increased spontaneous DNA damage, suggesting that Slx4 and Rtt107 function in concert. These defects are not shared by Slx1 indicating that the function of Slx4 and Slx1 in the DNA damage response is not entirely overlapping. Furthermore, I found that Slx4 regulates the phosphorylation of Rtt107 by the checkpoint kinase Mec1. The phenotypes conferred by deletion of RTT107 and the spectrum of its synthetic genetic interactions indicates that Rtt107 may function at stalled replication forks. I have shown that Rtt107 is recruited to chromatin in the presence of DNA damaging agents that cause DNA replication forks to stall. Recruitment of Rtt107 to chromatin requires Rtt109, an acetyltransferase, and the cullin Rtt101, but is not dependent on Slx4 or the checkpoint kinases. Rtt109 acetylates histone H3 on lysine 56 (H3-K56), yet recruitment of Rtt107 to chromatin does not require acetylation of H3-K56, indicating that Rtt109 may have additional targets. Chromatin immunoprecipitation indicates that the sites of Rtt107 binding correspond to regions at or near stalled replication forks throughout the genome. I propose that Rtt107 acts in the recovery from DNA damage by localizing to stalled replication forks and acting as a scaffold for assembly of DNA damage response proteins, ultimately promoting replication fork restart.

In Saccharomyces cerevisiae, RTT107 (ESC4, YHR154W) encodes a BRCT-domain protein that is important for recovery from DNA damage during S phase. I have found that Rtt107 forms a complex with the Slx1/Slx4 structure-specific nuclease. Deletion of SLX4 confers many of the same phenotypes observed in rtt107∆, including DNA damage sensitivity, prolonged DNA damage checkpoint activation, and increased spontaneous DNA damage, suggesting that Slx4 and Rtt107 function in concert. These defects are not shared by Slx1 indicating that the function of Slx4 and Slx1 in the DNA damage response is not entirely overlapping. Furthermore, I found that Slx4 regulates the phosphorylation of Rtt107 by the checkpoint kinase Mec1. The phenotypes conferred by deletion of RTT107 and the spectrum of its synthetic genetic interactions indicates that Rtt107 may function at stalled replication forks. I have shown that Rtt107 is recruited to chromatin in the presence of DNA damaging agents that cause DNA replication forks to stall. Recruitment of Rtt107 to chromatin requires Rtt109, an acetyltransferase, and the cullin Rtt101, but is not dependent on Slx4 or the checkpoint kinases. Rtt109 acetylates histone H3 on lysine 56 (H3-K56), yet recruitment of Rtt107 to chromatin does not require acetylation of H3-K56, indicating that Rtt109 may have additional targets. Chromatin immunoprecipitation indicates that the sites of Rtt107 binding correspond to regions at or near stalled replication forks throughout the genome. I propose that Rtt107 acts in the recovery from DNA damage by localizing to stalled replication forks and acting as a scaffold for assembly of DNA damage response proteins, ultimately promoting replication fork restart.

Also issued as a Ph.D. thesis in the Dept. of Electrical Engineering, 1973. / Bibliography: p. 51.

More than sixty years after Michael Ventris’ decipherment of the Linear B script, 14 of its 87 syllabic signs still have no sound-values assigned to them. This group of ‘undeciphered’ signs represent a significant gap in our ability not only to read the Linear B script, but also to understand its development and use. Chapter 1 of this thesis analyses the origins and usage of signs with known sound-values to establish what types of values are in principle most likely to be found amongst the undeciphered signs: this investigation also enables an exploration of the development of Linear B, its relationship with its parent script Linear A, and the motivating factors underlying the creation of new Linear B signs. Chapter 2 consists of studies of each individual undeciphered sign, including a palaeographic analysis of their forms, discussion of their corpus of attestations, and an examination of their prospects of decipherment and possible sound-values in the light of the results of Chapter 1. Finally, Chapter 3 employs this group of signs in a case-study to explore the potential of palaeographic analysis to contribute to our understanding of wider issues concerning the Linear B script and its context of use within the Mycenaean palaces. This case-study focuses in particular on two main uses of palaeography: as a means of assigning a relative chronology to Linear B texts, and as evidence for the reconstruction of the Mycenaean scribes’ administrative work and training.

Submitted by William Roberto Crestani (crestan@terra.com.br) on 2016-10-08T18:09:21Z No. of bitstreams: 2 Trabalho_de_Conclusão_-_Final_com_ficha_e_revisões_da_banca.PDF: 1167622 bytes, checksum: 185a7caee1c21b038191f9f990a2f3cf (MD5) Planilha - Análise decisões STF.XLSX: 61106 bytes, checksum: 6b299cb8a2c9554baca289b01aa8c047 (MD5) / Rejected by Renata de Souza Nascimento (renata.souza@fgv.br), reason: William, boa tarde Para que possamos aceitar seu trabalho junto à biblioteca, deverá realizar ajustes de acordo com as normas da ABNT. As páginas 1 até a página 4, deverão ser refeitas. Acessar o link http://sistema.bibliotecas-sp.fgv.br/bkab_normalizacao na opção Normas para apresentação de monografias, a partir da página 12 para verificar o modelo do trabalho. Ajustar também a numeração. Está correto a partir da Introdução, mas se por exemplo a Introdução for a página 9, incluir o numero 9 e seguir. Após ajustes, realizar nova submissão. Qualquer dúvida estamos à disposição. Att on 2016-10-10T17:35:24Z (GMT) / Submitted by William Roberto Crestani (crestan@terra.com.br) on 2016-10-11T03:36:06Z No. of bitstreams: 2 Dissertação - Mestrado Profissional - Limite das multas - versão biblioteca FGVpdf.pdf: 1166133 bytes, checksum: 160bb728f716fc4a0124bfa77ef7f446 (MD5) Anexo 2 - Planilha eletronica - Anexo II (Parte integrante da Dissertão - Mestrado Profissional).XLSX: 61109 bytes, checksum: 0136d49d91d71dbe6fa2f71f36048dbd (MD5) / Rejected by Renata de Souza Nascimento (renata.souza@fgv.br), reason: William, boa tarde Somente mais dois ajustes: Retirar a acentuação do nome Getúlio e retirar a sigla SP que consta ao lado de São Paulo (capa e contracapa). Att on 2016-10-11T17:09:31Z (GMT) / Submitted by William Roberto Crestani (crestan@terra.com.br) on 2016-10-11T17:59:13Z No. of bitstreams: 2 Dissertação - Mestrado Profissional - Limite das multas - versão final biblioteca FGV.pdf: 1166110 bytes, checksum: 2edafb6e1274e8d1eb4b4708fed3c0ef (MD5) Anexo 2 - Planilha eletronica - Anexo II (Parte integrante da Dissertão - Mestrado Profissional).XLSX: 61109 bytes, checksum: 0136d49d91d71dbe6fa2f71f36048dbd (MD5) / Approved for entry into archive by Renata de Souza Nascimento (renata.souza@fgv.br) on 2016-10-11T18:02:03Z (GMT) No. of bitstreams: 2 Dissertação - Mestrado Profissional - Limite das multas - versão final biblioteca FGV.pdf: 1166110 bytes, checksum: 2edafb6e1274e8d1eb4b4708fed3c0ef (MD5) Anexo 2 - Planilha eletronica - Anexo II (Parte integrante da Dissertão - Mestrado Profissional).XLSX: 61109 bytes, checksum: 0136d49d91d71dbe6fa2f71f36048dbd (MD5) / Made available in DSpace on 2016-10-11T18:22:09Z (GMT). No. of bitstreams: 2 Dissertação - Mestrado Profissional - Limite das multas - versão final biblioteca FGV.pdf: 1166110 bytes, checksum: 2edafb6e1274e8d1eb4b4708fed3c0ef (MD5) Anexo 2 - Planilha eletronica - Anexo II (Parte integrante da Dissertão - Mestrado Profissional).XLSX: 61109 bytes, checksum: 0136d49d91d71dbe6fa2f71f36048dbd (MD5) Previous issue date: 2016-09-13 / This paper is aimed at proposing a solution to the object matter of case 487 of the list of general repercussion of the Brazilian Supreme Court (STF), in which the court will judge the following subject: 487 – Confiscatory character of the 'isolated penalty' due for the non acomplishment of ancillary tax obligations. Based on the extensive examination of the STF precedents regarding the limitation of tax penalties, combined with the analysis of the applicable legal doctrine related to the tax sanctions and the proporcionality and reasonableness principles, we will develop and present an answer to the following questions: Is there any limitation to charge the penalty due for the non accomplishment of ancillary tax obligations in the event the tax was duly paid? If the answer is positive, which would be that limit? As a result, we are intended to face a recent ongoing problem in Brazil – which is the substantial increase on the amounts of the penalties charged for the non accomplishment of ancillary tax obligations, specially in cases in which taxes were duly paid by the taxpayers – and, simulteneously, contribute on the seek of a balance point between the function of the ancillary tax obligations and the amount charged as penalty for its non accomplishment. / Este trabalho tem por objetivo propor uma solução à questão objeto do tema 487 da lista de repercussões gerais do Supremo Tribunal Federal, por meio do qual o STF irá julgar a seguinte temática: 487 – Caráter confiscatório da 'multa isolada' por descumprimento de obrigação acessória decorrente de dever instrumental. A partir do exame extensivo da jurisprudência histórica do STF acerca da limitação das multas fiscais, combinado com o estudo da doutrina relativa às sanções tributárias e aos princípios da proporcionalidade e razoabilidade, desenvolveremos e apresentaremos ao final uma resposta objetiva às seguintes indagações: Existe algum limite que deve ser observado para a cobrança de multa pecuniária devida por descumprimento de dever instrumental nas situações em que não houve falta de recolhimento de tributo? Em caso positivo, qual seria esse limite? Desse modo, pretende-se enfrentar um problema que tem crescido no Brasil nos últimos anos – o aumento expressivo do valor das multas cobradas em razão do descumprimento do dever instrumental e, em vários casos, a desvinculação da cobrança dessa multa do próprio pagamento do tributo – e, simultaneamente, contribuir na tarefa de buscar um ponto de equilíbrio entre a função das obrigações tributárias acessórias e o valor da multa cobrada por conta do seu descumprimento.

Québec Université Laval, Bibliothèque 2013