The aim of this thesis is to suggest such measures which would optimize system of transportation in the National park Šumava. Regarding the fact that the area of examination is national park, principles of minimal load on environment has to be respected. In order to suggest optimization measures elements of transportation system are characterised and analyzed at first.

D.Phil. (Nematology) / This was the first study on aquatic nematodes from the Kruger National Park. Samples were taken more or less randomly over a period of two years from the four main rivers in the park, viz. the Crocodile, Sabie, Olifants and Luvuvhu rivers. A total of 33 species representative of 24 genera were found. Dorylaimida seemed to be dominant in most of the sampling sites in the Sabie, Olifants and Luvuvhu Rivers, but there was no dominant group in the Crocodile River. Five new Dorylaimida species are described: Dorylaimus deaconi spec. nov., Laimydorus olifanti spec. nov., Laimydorus africanus spec. nov.; proleptonchus krugeri spec. nov. and Aporcelaimellus glandus spec. nov. Previously known dorylaim species which were redescribed in detail are Mesodorylaimus sp.cf paralitoralis Basson & Heyns, 1974; M. potus Heyns, 1963, M. aegypticus (Andrassy, 1958), Aquatides thornei (Schneider, 1937), Afractinolaimus zairensis (Baqri, Coomans & Van der Heiden, 1975) and Oxydirus gangeticus Siddiqi, 1966. Further dorylaim species recorded are Aporcelaimellus micropunctatus Botha & Heyns, 1990, Discolaimus monoplanus Heyns, 1963, D. major Thorne, 1939, Xiphinema italiae Meyl, 1953, Thornenema baldum (Thorne, 1939), Tyleptus striatus Heyns, 1963 and Dorylaimellus (Axodorylaimellus) caffrae (Kruger, 1965) . The following Tylenchida species were found: Hemicycliophora labiata Colbran, 1969, Helicotylenchus africanus (Micoletzky, 1916) and Scutellonema brachyurum (Steiner, 1939). Three Araeolaimida species were recorded, viz. Chronogaster africana Heyns & Coomans, 1980, Plectus cirratus Bastian, 1865 and Rhabdolaimus terrestris de Man, 1880. The latter species is redescribed in full collected and (de Man, 1979). Only one species of the Cromadorida viz. Achromadora ruricola 1880), Enoplida viz. Eutobrilus annetteae (Joubert & Heyns, Monhysterida viz. Monhystera somereni Allgen, 1952, were Detailed descriptions are given to incorporate additional morphological information obtained. Brief descriptions and new distribution records are also given for Mononchus truncatus Bastian, 1865, M. aquaticus Coetzee, 1968 and Mylonchulus minor (Cobb, 1893) of the Mononchida and Ironus longicaudatus de Man, 1884 and I. ignavus Bastian, 1865 of the Iro~idae.A diagnostic compendium for the Mesodorylaimus species of South Africa and a checklist of the free-living and plant parasitic nematode species from both terrestrial and aquatic habitats in the park are also included. Most of this work has already been published or submitted for publication in scientific journals and is presented in this thesis in the same format as required by the editors of the journals concerned.

The aim of this Diploma Thesis is the elaboration of a study concerning the project for the reconstruction of the historical building of a granary in Hluboká nad Vltavou into an amusement park. The theoretical base of the thesis deals with the importance of the tourism at the national and regional levels where the contemporary situation of the Southern Bohemian Region is described in a more detailed way. A part of the thesis includes selected examples of local and foreign case studies dealing with similar projects like the planned project of an amusement park and it also corresponds with the contemporary market trends. The main practical part of the thesis deals with the design of the reconstruction of the historical building of a granary in Hluboká nad Vltavou into an amusement park. The purpose of the thesis is not the procession of a realistic project including all parts thereof but a study offering the solutions how to use the building in connection with the strategic planning document of the town Hluboká nad Vltavou as well as the Southern Bohemian Region. The practical part includes the description of the project of the granary, including the design, basic economic analysis and possibilities of the marketing communication. The technical documentation of the building is a part of attachments.

Analyzes of bat echolocation calls enable to investigate diverse patterns of bat communities without the need for contact manipulation with individuals. The continuous all-night acoustic recordings provide standardised data open to quantitative comparisons and testing effects of diverse contextual factors upon bat community structure. The multidisciplinary project MOSAIK (Monitoring Savanna Biodiversity in Kruger NP) mapping patterns of variation in savanna communities under different spatial and temporal influences includes bats as one of the model groups. At standardized monitoring points of the project (covering 20 different areas, each containing triplet points differing in access to the water surface: permanent, seasonal and the crests without a water source), all-night acoustic recordings of bats were undertaken over two seasons (using Song Meter recorders SM4BAT). All records were analyzed with aid of Kaleidoscope Pro software and cluster identification technique (with an input database developed by Weier et al. 2018 and Taylor et al. 2020) controlled by manual checking. Multiple comparisons of diverse coenologic variables of the particular samples were performed together with testing effects of associated contextual variables (geographic setting and climatic currents, seasons, vegetation,...

Residential Brno - Řečkovice A main task of this diploma thesis to propose the use of previosly undeveloped areas for the housing and also establish to the original structure of the village comunity by effective utilization of these areas. The ground is located in distrikt Řečkovice and Mokrá Hora in Brno. The Řečkovice cadastral is defined by Žilkova and Terezy Novákové street. North protperty line is determined by Ivanovice cadastral number 1035/26. The site is bounded by existing terraced and detached houses and apartment buildings on the northeast side. The south side is is formed by former barracks and northwest side provide open countryside towards Ivanovice. The development is designed to became a separate distrikt of Brno. The funcitonal areas are able to place residental house of different categories, also public facilities, recreation areas and public open space with all kinds of greenery. A main idea of the concept was to create residental comlex with a functional center that will not compete to existing center of Řečkovice. Densities are highest by the main road following the expected denser development of former barracks. They decreases ito the existing development along Žilkova street. The protective zone for pipeline is used as a city park. The proposed concept of functional areas clearly define continuity and transport infrastructure by design of buildings in different categories that meets all needs of future residents. Plenty of green space for recreation will also ensure functionality and efficient use of this unit.

Theileriosis, babesiosis, heartwater and anaplasmosis are considered to be amongst the most important tick-borne diseases of livestock in sub-Saharan Africa‟s tropical and subtropical regions resulting in extensive economic losses to farmers in endemic areas. It is well-known that the African buffalo (Syncerus caffer) is the natural reservoir host of various tick-borne haemoparasites of veterinary importance. In this study, the occurrence of tick-borne haemoprotozoan parasites (Theileria, Babesia, Anaplasma and Ehrlichia species) in buffalo from two geographically isolated national parks in South Africa (Kruger National Park and Hluhluwe-iMfolozi Park) was determined using the reverse line blot (RLB) hybridization assay. The RLB results revealed the presence of Theileria, Babesia and Anaplasma species either as single or as mixed infections. Although not detected with the RLB assay, 5% of the buffalo blood samples from the KNP tested positive for the presence of Ehrlichia ruminantium using the pCS20 real-time PCR assay. Previous studies on the occurrence of haemoparasites in the South African buffalo population have mainly focussed on the prevalence of Theileria species only. The finding on the presence of Anaplasma, Ehrlichia and Babesia species is therefore a novel contribution. This study has confirmed the findings of previous studies that buffalo is the natural reservoir host of both pathogenic and non-pathogenic Theileria species namely, T. parva, Theileria sp. (buffalo), T. mutans, T. velifera and T. buffe1i.In this study, the most frequently occurring Theileria species detected in the KNP were T. mutans (81%), Theileria sp. (sable) (61%), T. parva (40%), Theileria sp. (buffalo) (13%) and T. velifera (11%). Theileria buffeli was not detected in the KNP. In the Hluhluwe-iMfolozi Park, the most occurring Theileria species were T. mutans (55%), T. velifera (54%), T. parva (53%), Theileria sp. (sable) (53%), Theileria sp. (buffalo) (49%) and T. buffeli, (49%). Theileria sp. (sable) causes fatal clinical disease in roan and sable antelope in South Africa and we can only speculate whether the presence of Theileria sp. (sable) DNA in the buffalo population was a true and/or incidental finding. An interesting finding was the presence of Babesia occultans DNA in 50% of the buffalo from the Hluhluwe-iMfolozi Park. Babesia occultans is the causative agent of a benign form of cattle babesiosis in South Africa and, to date; this organism has not been identified in wildlife in South Africa. The significance of this finding warrents further investigation and confirmation using gene cloning, sequencing and phylogenetic analysis. Ehrlichia ruminantium has been reported to infect not only domesticated ruminants but also wild ruminants, however most wildlife species appear to carry the organism asymptomatically. In this study, we were not able to detect E. ruminantium DNA in any of the buffalo samples tested using the RLB hybridization assay. However, using the quantitative pCS20 real-time PCR assay we detected E. ruminantium DNA in 5% of the KNP samples. None of the Hluhluwe-iMfolozi Park samples tested positive for E. ruminantium using the real-time PCR assay. These results suggest that buffalo is not the natural reservoir host of E. ruminantium. However, a subclinical carrier state in buffalo has been experimentally shown to occur after tick transmission from carrier animals and further studies will have to be conducted to confirm whether this finding holds any potential risk to domestic animals. In Southern Africa, two Anaplasma species are known to infect cattle, A. marginale and A. centrale. Clinical bovine anaplasmosis is usually caused by A. marginale; whilst A. centrale generally results in mild disease. Because there is partial cross immunity between the two species, A. centrale is used as a live vaccine for cattle in Israel, South Africa, South America and Australia. Apart from cattle, Anaplasma marginale has been described in wild ruminants which can become persistently infected serving as reservoirs for infection of susceptible hosts; it has been recovered from 10 wild ruminants. Subclinical occurrence of A. marginale, either natural or after artificial infection has been confirmed in the African buffalo and various other wildlife species. In this study, the Anaplasma species detected from HluhluweiMfolozi Park buffalo samples were A. centrale (75%), A. marginale (42%) and Anaplasma (formerly Ehrlichia) sp. Omatjenne (28%). DNA of these species was also detected in buffalo from KNP; A. centrale (49%), A. marginale (24%) and Anaplasma (Ehrlichia) sp. Omatjenne (5%). The presence of A. marginale in the buffalo population suggests that buffalo may be a factor in the epidemiology and spread of bovine anaplasmosis because, as reservoir hosts of A. marginale, they could serve as a source of infective blood for mechanical spread by various routes and biological transmission by ticks. Factors such as climate, host abundance, tick host diversity, and topography have, however, all been shown to also impact on the epidemiology of A. marginale. Subsequently 64 samples were selected that either tested (i) positive for a specific Anaplasma spp. (A. centrale, A. marginale and/or Anaplasma (Ehrlichia) sp. Omatjenne) using the RLB assay, or (ii) in which the PCR products hybridized only with the Anaplasma/Ehrlichia genus-specific probes for molecular characterization by cloning and sequencing of the 16S rRNA gene. Aplification of the full-length and/or partial parasite 16S rRNA gene of any of the selected samples that previously tested positive for the presence of Anaplasma (Ehrlichia)sp. Omatjenne (using the RLB assay) or E. ruminantium (using the pCS20 real-time PCR assay) was unsuccessful. This was most probably due to low rickettsaemia. However, amplification of either the near full-length parasite 16S rRNA gene or a partial 16S rRNA gene from seven samples from the KNP and three from Hluhluwe-iMfolozi Park was successful. Results indicated that the obtained sequences of 12 of the 18 clones were highly similar to published A. centrale 16S rRNA gene sequences, four of the clones were highly similar to the published A. marginale sequences and the sequences of the remaining two clones were closely similar to Anaplasma (Ehrlichia) sp. strain Omatjenne. The observed sequence similarities were confirmed by phylogenetic analyses. An interesting finding was the presence of one full-length parasite 16S rDNA sequence that was 100% identical to that of the published A. centrale vaccine strain sequences. It is well known that A. centrale is widely used as live vaccine for the control of bovine anaplasmosis. The occurrence of A. centrale vaccine strain DNA in the South African buffalo population is therefore of great interest. It can only be speculated whether A. centrale has evolved in the African buffalo, and/or if buffalo act as natural reservoir hosts, or if is it merely being maintained in the buffalo population by in utero transmission. This also serves as the first report of Anaplasma (Ehrlichia) sp. Omatjenne DNA in the African buffalo which warrents further investigation. In conclusion, the findings suggest that buffalo is a natural reservoir of Anaplasma spp. infection and could play an important role in the epidemiology and spread of anaplasmosis and may represent a serious threat to the livestock industry. / Dissertation (MSc)--University of Pretoria, 2012. / Veterinary Tropical Diseases / unrestricted

京都大学 / 新制・課程博士 / 博士(農学) / 甲第23390号 / 農博第2463号 / 新制||農||1086(附属図書館) / 学位論文||R3||N5347(農学部図書室) / 京都大学大学院農学研究科森林科学専攻 / (主査)教授 北島 薫, 教授 神﨑 護, 准教授 三谷 羊平 / 学位規則第4条第1項該当 / Doctor of Agricultural Science / Kyoto University / DGAM

This research is an exploratory investigation of the potential of New Urbanist planning and design principles to create thriving and successful neighborhoods. New Urbanism is an urban design movement started in the early 1980s that promotes the development of walkable, compact, and diverse neighborhoods. It is the objective of this research to shed light on the value of creating a higher quality of life and more time for ourselves and our families. I hypothesize that New Urbanism may pave the way for recapturing commute time for time with family, creating authentic and successful communities, and engaging with neighbors. Furthermore, by utilizing the design principles of New Urbanism, traditional neighborhoods that re-integrate the activities of daily living may be possible. In short, this research is an explanatory and exploratory investigation that examines whether incorporating the design principles of New Urbanism can create walkable and successful communities that promote community-making with urban patterns that facilitate knowing neighbors, communication among neighbors, community activities, and a long term sense of belonging. This research focuses on three case studies of New Urbanism: a large scale development in Atlanta, Georgia with approximately 3,500 residents, a moderate scale development in Tampa, Florida with approximately 2,000 residents, and a small scale development in Fernandina Beach, Florida with approximately 700 residents. These three case studies provide successful examples of the integration of the design principles of New Urbanism. New Urbanism can be used as the stimulus for the types of neighborhoods Americans has been missing since World War II. At each of these sites, observations were made of the ways in which the design of the development has fostered community and created walkable and livable neighborhoods.

Legal aspects of tourism and sport in Krkonoše mountains national park Abstract This presented thesis analyse law regulation concerning protection of National Parks in against negative Influence of tourism and sport. Assessing impact of tourism on essential pillars (principles) of sustainable development I tried to find out if the preservation and protection of national Parks in The Czech Republic within Czech legislation is adequate. Content of the thesis of eight chapters is definition of terminology used in this thesis, listing of sources of legal regulation and means of protection of nature at National Parks and comparison of specific legal regulation of KRNAP and KPN. At the end of thesis the essential findings of the analyses are summarised. The aim of this thesis is to find a compromise between effective legal regulation and over-regulation of tourist and sport activities in National Parks. Key words: national park, tourism, legal protection, environment

The subject of this diploma thesis is the location and design of a theater building in the body of a regional center - the town of Uherské Hradiště. The existing theater building is housed in a rented Sokolovna (sport-cultural building) on Tyršovo Square. There is also a small stage in a house on Mariánské náměstí. The capacity of the auditorium of the large scene is 370 spectators, corresponding to the needs of the city and the region. However the size of the stage is completely inadequate, the height of the flytower, the absence of back and side stages, Also, the workshops and warehouses are currently located away from the theater. The project addresses the current unfavorable situation of the theater, which is, in the long-term, interest to the city of Uherské Hradiště itself.