No description available.

No description available.

Terroir combines the physical landscape of the vineyard with the grapevines and the methods and techniques used to produce wine from the grapes. This study used a GIS to identify the characteristics of the physical landscape in Pottawattamie, Mills, Montgomery, Fremont, and Page counties in southwestern Iowa. The components were combined in the GIS using a weighted linear index to identify areas suitable for vineyard development and to identify the general characteristics of the area. Vineyard owners were interviewed to help determine the weighting system to use in the GIS and to determine their perceptions of how the physical landscape impacts their vineyards, as well as to determine what grape varieties they plant in their vineyards and their decisions on making wine from these grapes. This information was collected to identify whether the vineyard owners had developed a sense of place for their vineyards and how this sense might aid them in the development of a terroir for their wines. The resulting perceptions about the individual wineries were then considered in conjunction with the results from the GIS modeling to understand how the physical landscape influences the concepts of sense of place and terroir in southwest Iowa. The physical landscape of southwest Iowa was fairly uniform, as were the grape varietals planted in the vineyards. This created a measure of similarity among the wineries, while individuality between wineries was then created by the wine-makers as they used different techniques to produce wine from the grapes. This allows each winery to develop a sense of place, yet be part of a larger sense of place that encompasses multiple wineries within the area.

No description available.

Made available in DSpace on 2018-08-01T23:30:08Z (GMT). No. of bitstreams: 1 tese_10310_Polem.pdf: 10659056 bytes, checksum: c554e8c832228940cdde565a4553b789 (MD5) Previous issue date: 2016-09-23 / A presente dissertação explora questões sobre tradução e práticas artísticas contemporâneas pensadas de maneira site specific, investigando se é possível criar um paralelo entre os processos de tradução e os processos de apropriação de um site para criação de um trabalho artístico. Para isso, foram estudados aspectos relevantes na mudança dentro do campo das artes plásticas a partir do final da década de 1950, além de ter sido feito um breve levantamento histórico sobre as questões relativas ao lugar e o aparecimento das práticas site specific. Os problemas no que diz respeito à tradução foram discutidos a partir de um apanhado de teorias relativas ao processo tradutório, bem como estudos de traduções pensadas de maneira livre. O conceito de tradução, portanto, tentou ser ampliado e, para esta pesquisa, foi pensado em campos além da lingüística, trabalhando com diferentes linguagens. Para esta dissertação, considerou-se o tradutor como um criador e assim, foi possível relacionar a tradução com as práticas contemporâneas ditas site specific, atentando-se para o site como um lugar de discurso. Do mesmo modo em que um artista se apropria de um site para pensar no seu trabalho, podendo trabalhar com diferentes linguagens em uma mesma obra, os tradutores também concentram-se em se apropriarem de contextos para criarem suas traduções. A partir desta pesquisa teórica inicial, buscou-se dois artistas contemporâneos - Raquel Garbelotti e Antoni Muntadas - para analisar de que maneira artistas-tradutores, que pensam de modo site specific, criam seus trabalhos. PALAVRAS-CHAVE: lugar; tradução; site specific; transcriação;

Graduation date: 1998

Thesis (M.Ed.)--Regis University, Denver, Colo., 2006. / Title from PDF title page (viewed on Feb. 20, 2007). Includes bibliographical references.

Thesis (Ph. D.)--Oregon State University, 1983. / Typescript (photocopy). Includes bibliographical references (leaves 202-218). Also available on the World Wide Web.

In recent years, on-site analysis has garnered increased interest from the scientific community. The development of smaller, more sophisticated analytical instruments, and the establishment of new environmental regulations have encouraged the application of new methodologies for field analysis. Prominent advantages of on-site analysis include elimination of error sources due to sample transportation and matrix modification, considerable reduction in analysis time, and more accurate and precise analytical results. Several techniques suitable for on-site analysis, which integrate sampling and sample preparation in one step, have demonstrated high versatility and throughput in field applications. This research was focused on the application and evaluation of three microextraction techniques: solid phase microextraction (SPME), needle trap devices (NTD) and membrane extraction with sorbent interface (MESI), which were then coupled with various portable instruments for on-site analysis of different systems. Additionally, the conducted project involved the development of an approach using ion mobility spectrometry detection (IMS) coupled with a miniaturized gas chromatograph (GC) as a powerful system for field analysis. This proposed GC-IMS exhibited satisfactory performance in terms of retention time (inter-day variation < 3%) and response stability (intra and inter-day relative standard deviations (RSDs) < 10 %). Moreover, when coupled with NTD, it showed limits of detection comparable to those provided by conventional benchtop instruments. Other portable GC instruments employed in this project included flame ionization and mass spectrometry detection. Three different sample systems were investigated using SPME and NTD together with these portable instruments: emissions of a pine branch, breath samples, and indoor pollutants in a polymer synthesis laboratory. Consequently, the feasibility of using SPME and NTD for determination of free and total concentrations was investigated. Finally, MESI was successfully coupled with the newly proposed GC-IMS system, and its functionality was evaluated by analyzing acetone in breath samples.

Hox proteins are evolutionarily conserved transcription factors that control important developmental pathways in morphogenesis of the embryo. The Hoxa2 gene is expressed in the developing central nervous system in rhombomeres 2 to 7 and affects cellular differentiation. Few target genes of Hoxa2 protein have been identified so far and its mechanisms of regulating gene expression remain elusive. Previous work in our laboratory isolated Hoxa2 protein binding sequences from the E18 mouse spinal cord and hindbrain tissues using chromatin immunoprecipitation (ChIP). All isolated DNA fragments contain conserved GATG motifs. Sequence analysis revealed that one fragment belongs to the high temperature requirement factor A 3 (HtrA3) gene and another fragment belongs to the Dual specificity tyrosine kinase 4 (Dyrk4) gene. In this study, direct binding of Hoxa2 protein to the HtrA3 and Dyrk4 fragments was confirmed by electrophoretic mobility shift assays (EMSA). Site-directed mutagenesis and EMSA studies revealed that Hoxa2 protein binds to the multiple GATG motifs within these fragments. HtrA3 fragment also repressed luciferase gene expression in transient transfection and luciferase assays. Mutation of the DNA fragment showed that the repressive activity was affected by the GATG motifs, suggesting Hoxa2 protein regulated gene expression by binding to the GATG motif in the cis-regulatory element. In contrast to the inhibitory activity of Hoxa2 protein, a Hoxa2-VP16 fusion protein (Hoxa2 fused with an activation domain of a virion protein from herpes simplex virus) transactivates the luciferase expression by binding to GATG sites. RT-PCR and immunohistochemistry analysis revealed an upregulation of HtrA3 expression in Hoxa2-/- mice. This observation correlates with the inhibitory role of Hoxa2 protein acting upon the HtrA3 fragment in luciferase assays. Our data suggest that HtrA3 is a direct in vivo downstream target of Hoxa2 protein and support the activity regulation model in which Hox proteins selectively regulate target genes through occupation of multiple monomer binding sites.