Orientador: Eliana Aparecida Varanda / Banca: Denise Crispim Tavares / Banca: Miriam Sannomiya / Banca: Lourdes Campaner dos Santos / Banca: Taís Maria Baub / Resumo: O presente trabalho teve como objetivo estudar quatro espécies relacionadas no Projeto BIOTAFAPESP, as quais têm se destacado quanto às atividades farmacológicas: Byrsonima fagifolia Niedenzu, Byrsonima crassa Niedenzu, Indigofera truxillensis Kunth e Indigofera suffruticosa Miller. Foram realizados ensaios de mutação gênica reversa utilizando-se as linhagens TA98, TA100, TA97a e TA102 de Salmonella typhimurium, com ausência e presença do sistema metabólico de ativação. Foram avaliados extratos, frações e algumas substâncias isoladas, a saber: a) B. crassa: amentoflavona, substância isolada do extrato metanólico; b) B. fagifolia: extrato metanólico e clorofórmico, frações acetato e aquosa, ácido gálico, galato de metila, ácido quínico e o ácido 3,4- digaloilquínico; c) I. truxillensis e I.suffruticosa: extrato metanólico e clorofórmico, frações de alcalóides, flavonóides e de glicerolipídeos, além das substâncias índigo, indirubina e o kaempferol- 3,7-diraminosídeo. Foi também avaliada a isatina, molécula precursora dos alcalóides índigo e indirubina. As atividades anti-bacteriana, anti-Leishmania, citotóxica e fitoestrogênica foram avaliadas para substâncias isoladas. Os ensaios de atividade fitoestrogênica foram realizados através do método de e-screen, utilizando-se células tumorais MCF7. Os ensaios de atividade citotóxica (células MCF-7) foram realizados pela técnica de sulforadamina-B. Os ensaios anti- Leishmania (L. amazonensis) também foram relizados com as técnicas de MTT (sal de tetrazólio) e contagem manual em câmara de Neubauer. A atividade anti-bacteriana foi avaliada através das técnicas de difusão em agar e microdiluição, com as bactérias Staphylococcus aureus ATTC 25923 e Escherichia coli ATCC 25922. Estudos anteriores evidenciaram o potencial... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The present work aimed to investigate four related species in the project BIOTA-FAPESP which are notable for their pharmacological activities: Byrsonima fagifolia Niedenzu Byrsonima crassa Niedenzu, Indigofera truxillensis Kunth and Indigofera Indigofera suffruticosa Miller. Tests of reverse gene mutation were performed using TA98, TA100, TA97 and TA102 strains of S. typhimurium in the absence and presence of metabolic activation system. Extracts, fractions and some isolated compounds were evaluated: a) B. fagifolia: methanolic and chloroformic extracts, acetate and aqueous fractions, gallic acid, methyl gallate, quinic acid and 3,4-digaloilquinico derivative. b) I. truxilensis and I.suffruticosa: chloroformic and methanolic extracts, fractions of alkaloids, flavonoids and glicerolipides, and also the indigo, indirubin and kaempferol-3,7-diraminosídeo substances. The isatin, precursor molecule of alkaloids indigo and indirubin was also evaluated. The antimicrobial, antiparasitic, cytotoxic and phytoestrogenic activities were evaluated for natural isolated substances. The phytoestrogenic activity assays were performed using the e-screen method, using MCF7 tumor cells. Trials of cytotoxic activity (MCF-7 cells) were done using sulforadamina-B. The anti-Leishmania tests (L. amazonensis) were also performed with some alkaloids and flavonoids, using MTT techniques (tetrazolium salt) and manual counting in a Neubauer chamber. Antimicrobial activity was evaluated using disc diffusion techniques and microdilution with S. aureus ATTC 25923 and E. coli ATCC 25922 bacterias. The mutagenic activity assays showed positive results only for the methanol extract of I. truxilensis (strain TA98) and for the alkaloids indirubin and indigo isolated from this specie and I. suffruticosa, in addition to isatin. This precursor is also noted... (Complete abstract click electronic access below) / Doutor

Esta pesquisa teve como finalidade conhecer o trabalho docente dos professores de formação técnica que atuam na educação profissional e tecnológica (EPT) oferecida nos Institutos Federais de Educação, Ciência e Tecnologia sob a perspectiva do conceito de atividade oriundo da Psicologia Histórico-cultural, da Teoria da Atividade e da Clínica da Atividade. O objetivo geral é analisar a atividade do professor de formação técnica em Informática que atua na educação profissional e tecnológica. Os objetivos específicos são: conhecer as trajetórias profissionais e formativas dos docentes de formação técnica em Informática que atuam na educação profissional e tecnológica e que serão os interlocutores da pesquisa; compreender quais articulações estes professores fazem entre as experiências que vivenciaram como profissionais de suas áreas de formação inicial e as demandas da docência na educação profissional e tecnológica; identificar quais as prescrições e a presença delas na atividade destes professores; e explorar o significado e o sentido pessoal do trabalho docente para os interlocutores da pesquisa. A Clínica da Atividade foi utilizada como aporte teórico e como método e as informações foram produzidas através de instrução ao sósia, autoconfrontações simples e cruzadas, observação participante e entrevistas intensivas. Participaram da pesquisa dois docentes da área de Informática que atuam em câmpus de dois institutos federais do Rio Grande do Sul. Depois da realização de todas as etapas que constituíram a pesquisa, a tese construída é que os docentes pesquisados realizam uma atividade de ensino voltada para a orientação e preparação profissional que visa que os alunos se apropriem do acúmulo de cultura de área de Informática e possam exercer essa profissão. Os docentes agem através dos gêneros profissionais da docência e do campo de atuação em Informática e há indícios que demonstram que estes docentes produzem um novo gênero profissional ao qual imprimem os seus estilos. Esse novo gênero não é a negação de nenhum dos gêneros profissionais nos quais os docentes se movimentam, mas, sim, a confluência deles na criação de uma nova atividade real que assume as prescrições de ambas, encadeando-as, cruzando-as e equilibrando-as. Nessa atividade, os docentes esbarram em duas grandes questões que compõem o real da atividade: a dificuldade de adaptar o ensino de uma profissão aos tempos escolares e o desconhecimento de outras formas de ensinar pautadas mais na promoção de atividades de estudo aos alunos e menos em tarefas de reprodução do fazer do professor. Conhecer a atividade dos professores a partir da Clínica da Atividade pode colaborar com a construção de formações iniciais e continuadas que respeitem as especificidades da docência na educação profissional e promovam o aumento do poder de agir dos docentes diante de seu próprio trabalho. / This research aimed to know the work of the technical training of teachers who work in professional and technological educational (PTE) offered in the Federal Institutes of Education, Science and Technology under the perspective of the concept of activity originating from the Cultural-historical Psychology of Activity Theory and Activity Clinic. The general objective is to analyze the activity of the teacher that has technical training engaged in professional and technological education. The specific objectives are: to know the professionals and formative trajectories of the technical teachers training in Information Technology that work in professional and technological education and who will be the interlocutors of the research; to understand what interligations these teachers make between the experiences that lived as professionals in their areas of initial training and the demands of teaching in professional and technological educational; to identify the prescriptions and their presence in the activity of these teachers; and explore the meaning and personal sense of the teaching work to the interlocutors of the research. The Activity Clinic was used as a theoretical and methodological contribution and the information was produced through instruction to the look-alike, simple and crossed self-confrontations, participant observation and intensive interviews. Participated in the research two teachers of Information Technology area that work on campus of two federal institutes of Rio Grande do Sul. After the completion of all the steps that constitute the research, the built thesis is that the researched teachers perform a focused education activity for orientation and professional training that aims to students to take as of the culture of Computer Science area and to exercise this profession.The teachers act through the professional genres of teaching and of the field of action in Informatics and there are indications that show that these teachers produce a new professional type to which they imprint their styles. This new genre is not the denial of any of the professional genres in which teachers move, but rather the confluence of them in the creation of a new real activity that assumes the prescriptions of both, chaining them, crossing them and balancing them. In this activity, the teachers have two issues constitute the real activity: the difficulty of adapting teaching a profession to school organization and the lack of knowledge of other forms of teaching focused on the promotion the studying activities and less on the reproductions of the teacher’s act. Know the teachers activity from the Activity Clinic can collaborates with building of the initial and continued education that respect the characteristics of teaching in professional and technological educational and increase the teachers’ power to act in their own work space.

Submitted by JULIA DIETSCHE MONFARDINI null (juliadm@iq.unesp.br) on 2016-04-01T16:52:10Z No. of bitstreams: 1 Dissertação_Julia Dietsche Monfardini.pdf: 10246419 bytes, checksum: dff6716d7f1e0f36c408ba7afeff1e25 (MD5) / Approved for entry into archive by Ana Paula Grisoto (grisotoana@reitoria.unesp.br) on 2016-04-05T18:12:36Z (GMT) No. of bitstreams: 1 monfardini_jd_me_araiq_par.pdf: 807325 bytes, checksum: 9a9635608b6709f23a60f0fd6f5e676e (MD5) / Made available in DSpace on 2016-04-05T18:12:36Z (GMT). No. of bitstreams: 1 monfardini_jd_me_araiq_par.pdf: 807325 bytes, checksum: 9a9635608b6709f23a60f0fd6f5e676e (MD5) Previous issue date: 2016-03-10 / Os micro-organismos têm demonstrado serem valiosas fontes de princípios ativos de uso clínico, no qual a penicilina é o exemplo mais conhecido de metabólito secundário, de importância medicinal, produzido por fungos. A espécie vegetal Eugenia jambolana Lam., popularmente conhecida como Jambolão, é utilizada na medicina popular brasileira, principalmente no tratamento de diabetes. O fungo Botryosphaeria parva, isolado das folhas da E. jambolana (L.) e codificado como Ej_F01, foi cultivado em escala ampliada em três meios de cultivo: Czapek®, PDB e Milho. Após o período de fermentação do endófito no meio de milho, foi feita uma extração direta com MeOH, seguida de filtração e evaporação do solvente. O extrato obtido foi solubilizado em AcOEt e fez-se três partições líquido-líquido com H2O, após a evaporação do solvente orgânico, o extrato foi dissolvido em CH3CN e foi feita uma nova partição líquido-líquido com Hexano, após a evaporação da CH3CN, foi obtido o extrato de interesse. Após o período de fermentação do endófito nos meios líquidos (PDB e Czapek®), a suspensão micelar foi filtrada a pressão reduzida, separando os micélios dos meios fermentados. Em seguida, os filtrados aquosos foram submetidos a três partições líquido-líquido com AcOEt, e após a evaporação do solvente orgânico, foram obtidos os extratos brutos de interesse. Todos os extratos foram fracionados utilizando técnicas cromatográficas, como Cromatografia em Coluna e/ou Cromatografia Líquida de Alta Eficiência preparativa. Após o fracionamento dos extratos brutos, foi possível obter sete substâncias oriundas do extrato do milho, seis substâncias do extrato do Czapek®, e uma do extrato de PDB, que foram submetidas a análises espectrométricas (EM, RMN de 1H, 13C, 1D e 2D) para a determinação/identificação estrutural. As substâncias 05, 08, 09 e 11 foram identificadas como 5-hidroximeleina, rel. (3S, 4S)-4-hidroximeleina, meleina e rel. (3S, 4R)-4-hidroximeleina, respectivamente, pertencentes à classe das isocumarinas; as substâncias 01, 03, 06 e 07 foram identificadas como botryosphaerin A, 13,14,15,16-tetranorlabd-7-eno-19,6β:12,17-diolídeo, CJ-14445 e oidiolactona E, respectivamente, pertencentes à classe dos diterpenos tetranorlabdanos; a substância 12 foi identificada como botryosphaerona D, pertencente à classe das naftalenonas. A substância 02, inédita, foi nomeada como rel. (4S, 5R, 6R, 10S)-18-hidroxi-13,14,15,16-tetranorlabd-7,9-dieno-19,6β:12,17-diolídeo, codificada como botryosphaerin I. Os estudos relacionados ao B. parva têm como finalidade verificar sua produção metabólica em diferentes meios de cultivo, bem como a avaliação do potencial biológico dos extratos brutos, frente aos ensaios antioxidante, antifúngico e anticolinesterásico. / Microorganisms have proven to be valuable sources of active principles for clinical use, wherein the penicillin is the best known example of a secondary metabolite of medicinal importance, produced by fungi. The plant species Eugenia jambolana Lam., commonly called Black Plum, is used in traditional Brazilian medicine, especially in the treatment of diabetes. The Botryosphaeria parva fungus, isolated from leaves of E. jambolana (L.) encoded as Ej_F01, was grown on an enlarged scale in three different culture media: Czapek®, PDB and corn. After the fermentation period in the corn media, a direct extraction was done with MeOH followed by filtration and evaporation of the solvent. The extract was disolved in AcOEt and made three liquid-liquid partition with H2O, after evaporation of the organic solvent, the extract was dissolved in CH3CN and was made a new liquid-liquid partition with Hexane, after evaporation of the CH3CN, it was obtained extract of interest. After the fermentation period in liquid media (PDB and Czapek®), the micellar suspension was filtered under reduced pressure, separating the mycelium from the fermented broth. Then, the aqueous filtrates were subjected to three liquid-liquid partition with AcOEt, and after evaporation of the organic solvent, there were obtained the crude extracts of interest. All extracts were fractionated using chromatographic techniques such as column chromatography and/or preparative High Performance Liquid Chromatography efficiency. After the fractioning of crude extracts, it was possible to obtain seven substances derived from corn extract, six substances obtained from Czapek extract, and a substance obtained from the PDB extract, which were submitted to spectrometric analysis (MS-ESI, NMR 1H, 13C, 1D and 2D) for determining and structural identification. The substances 05, 08, 09 and 11 were identified as 5-hydroxymelein, rel. (3S, 4S)-4-hydroxymelein, Melein and rel. (3S, 4R)-4-hydroxymelein, respectively, belonging to the class of isocoumarins; the substances 01, 03, 06 and 07 were identified as botryosphaerin A, 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide, CJ-14445 and oidiolactone E, respectively, belonging to the class of tetranorlabdanes diterpenoids, and substance 12 was identified as botryosphaerone D, belonging to the class of naftalenones. A new tetranorlabdane diterpenoid was obtained, substance 02, named rel. (4S, 5R, 6R,10S)-18-hydroxy-13,14,15,16-tetranorlabd-7,9-dien-19,6β:12,17-diolide and encoded botryosphaerin I. These studies related to the B. parva are intended to verify your metabolic production in different culture media as well as the evaluation of the biological potential of extracts compared to antioxidant, antifungal and anticholinesterase trials.

Esta pesquisa teve como finalidade conhecer o trabalho docente dos professores de formação técnica que atuam na educação profissional e tecnológica (EPT) oferecida nos Institutos Federais de Educação, Ciência e Tecnologia sob a perspectiva do conceito de atividade oriundo da Psicologia Histórico-cultural, da Teoria da Atividade e da Clínica da Atividade. O objetivo geral é analisar a atividade do professor de formação técnica em Informática que atua na educação profissional e tecnológica. Os objetivos específicos são: conhecer as trajetórias profissionais e formativas dos docentes de formação técnica em Informática que atuam na educação profissional e tecnológica e que serão os interlocutores da pesquisa; compreender quais articulações estes professores fazem entre as experiências que vivenciaram como profissionais de suas áreas de formação inicial e as demandas da docência na educação profissional e tecnológica; identificar quais as prescrições e a presença delas na atividade destes professores; e explorar o significado e o sentido pessoal do trabalho docente para os interlocutores da pesquisa. A Clínica da Atividade foi utilizada como aporte teórico e como método e as informações foram produzidas através de instrução ao sósia, autoconfrontações simples e cruzadas, observação participante e entrevistas intensivas. Participaram da pesquisa dois docentes da área de Informática que atuam em câmpus de dois institutos federais do Rio Grande do Sul. Depois da realização de todas as etapas que constituíram a pesquisa, a tese construída é que os docentes pesquisados realizam uma atividade de ensino voltada para a orientação e preparação profissional que visa que os alunos se apropriem do acúmulo de cultura de área de Informática e possam exercer essa profissão. Os docentes agem através dos gêneros profissionais da docência e do campo de atuação em Informática e há indícios que demonstram que estes docentes produzem um novo gênero profissional ao qual imprimem os seus estilos. Esse novo gênero não é a negação de nenhum dos gêneros profissionais nos quais os docentes se movimentam, mas, sim, a confluência deles na criação de uma nova atividade real que assume as prescrições de ambas, encadeando-as, cruzando-as e equilibrando-as. Nessa atividade, os docentes esbarram em duas grandes questões que compõem o real da atividade: a dificuldade de adaptar o ensino de uma profissão aos tempos escolares e o desconhecimento de outras formas de ensinar pautadas mais na promoção de atividades de estudo aos alunos e menos em tarefas de reprodução do fazer do professor. Conhecer a atividade dos professores a partir da Clínica da Atividade pode colaborar com a construção de formações iniciais e continuadas que respeitem as especificidades da docência na educação profissional e promovam o aumento do poder de agir dos docentes diante de seu próprio trabalho. / This research aimed to know the work of the technical training of teachers who work in professional and technological educational (PTE) offered in the Federal Institutes of Education, Science and Technology under the perspective of the concept of activity originating from the Cultural-historical Psychology of Activity Theory and Activity Clinic. The general objective is to analyze the activity of the teacher that has technical training engaged in professional and technological education. The specific objectives are: to know the professionals and formative trajectories of the technical teachers training in Information Technology that work in professional and technological education and who will be the interlocutors of the research; to understand what interligations these teachers make between the experiences that lived as professionals in their areas of initial training and the demands of teaching in professional and technological educational; to identify the prescriptions and their presence in the activity of these teachers; and explore the meaning and personal sense of the teaching work to the interlocutors of the research. The Activity Clinic was used as a theoretical and methodological contribution and the information was produced through instruction to the look-alike, simple and crossed self-confrontations, participant observation and intensive interviews. Participated in the research two teachers of Information Technology area that work on campus of two federal institutes of Rio Grande do Sul. After the completion of all the steps that constitute the research, the built thesis is that the researched teachers perform a focused education activity for orientation and professional training that aims to students to take as of the culture of Computer Science area and to exercise this profession.The teachers act through the professional genres of teaching and of the field of action in Informatics and there are indications that show that these teachers produce a new professional type to which they imprint their styles. This new genre is not the denial of any of the professional genres in which teachers move, but rather the confluence of them in the creation of a new real activity that assumes the prescriptions of both, chaining them, crossing them and balancing them. In this activity, the teachers have two issues constitute the real activity: the difficulty of adapting teaching a profession to school organization and the lack of knowledge of other forms of teaching focused on the promotion the studying activities and less on the reproductions of the teacher’s act. Know the teachers activity from the Activity Clinic can collaborates with building of the initial and continued education that respect the characteristics of teaching in professional and technological educational and increase the teachers’ power to act in their own work space.

INTRODUCTION: Direct observation (DO) systems have been used for decades to assess free-living PA in children. These traditional DO systems identify the highest intensity observed during alternating observe-and-record periods. Using video-taped DO would allow researchers to code activities and contextual information each time the participant changes their behavior. PURPOSE: To develop and test a novel video-based DO system for children’s free-play activity. METHODS: Following iterative DO system development (The Observer XT, Noldus), 28 children (age=8.4 ± 1.5 years) participated in a 30-minute simulated free-play session that was recorded with a GoPro camera. Participants wore a portable indirect calorimetry (IC) device and an accelerometer on the hip (AG-H) and non-dominant wrist (AG-W). The DO system includes Whole Body Movement (body position, main movement pattern) that was further described with four modifiers: 1) Locomotion, 2) Limb Movement, 3) Activity Type, and 4) MET value. To assess intrarater reliability, an expert coder coded six randomly selected videos from the main sample and recoded the same videos one week later. Six novice coders were trained and coded three videos from the subsample to assess interrater reliability. To assess construct validity, total energy expenditure and time spent in activity intensity categories from DO were compared with IC and accelerometer estimates. RESULTS: Percent agreement for intrarater reliability was above 80% except for Locomotion (47%; video 3) and Limb Movement, MET value and Locomotion (19%, 78%, 26%), respectively, video 4). Across all variables, percent agreement for interrater reliability ranged widely from 12%-96%, 0-100%, and 36%-97% for videos 1, 2 and 3, respectively. Mean estimated time spent in PA intensity categories from AG-H overestimated sedentary (SED) and underestimated light, moderate, and moderate-to-vigorous PA (LPA, MPA, and MVPA; p < 0.001-0.008). AG-W and IC underestimated SED (p=0.03, p=0.03) and LPA (p< 0.001, pONCLUSION: The current DO system is feasible for observing detailed changes in children’s free-play activity. However, refinement to the system must be made to improve reliability before it is adopted as a criterion measure for free-play activity in children.

Urginea sanguinea, Aloe marlothii, Elephantorrhiza elephantina and Rhoicissus tridentate are all plants utilized for the management of tick borne diseases in the Madikwe area of North-west province. These plants, in certain concoctions, are believed to be effective against “seme”, “gala” and “Bolwetsi jwa mothlapo o moshibidu” which we have assumed to represent heartwater, gallsickness and redwater from circumstantial epidemiological data available. To obtain a representative extract, which would be indicative of the general activity of the plant, only acetone or methanol extracts were tested for the presence of antimicrobial, antiparasitic or anti-oxidant activity within that specific plant. Activity in all cases made use of either an in vitro biological assay or more specific chemical tests, which were validated in all cases. Ehrlichia ruminantium, Babesia caballi and Theileria equi, all grown in specific cell cultures, were used as a model for evaluating the efficacy against the common protozoan and rickettsial diseases caused by these organisms in livestock. Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli, four human nosocomial infectious agents, were used as an indicator for the presence of antibacterial activity against these common animal bacterial pathogens. Diphenyl-picrylhydrazyl and the trolox equivalent anti-oxidant chemical assays were used to determine anti-oxidant activity, which although not curative, may aid in the recovery from an infection by stimulating the immune system. The activities demonstrated among the various plants and organisms were not consistent. E. elephantine extracts were the most effective, with activity demonstrable in all biological and chemical screening assays. Although R. tridentate demonstrated poor activity (> 100 ìg/ml) against the tick-borne parasites, the plant extract did demonstrate significant anti-oxidant activity. U. sanguinea extracts showed good activity in both the antibacterial and anti-rickettsial assays (EC50 = 44.49 ng/ml), which may be due to the presence of the toxic bufadienolides present within the plant. A. marlothii possessed significant anti-rickettsial activity (EC50= 111.4 µg/ml) and to a lesser degree antibacterial activity. The results of the study support the use of these plants against heartwater, gallsickness and redwater, which gives credence for the traditional use against “Seme, Gala, and Bolwetsi jwa mothlapo o moshibidu”. Further studies are required to isolate and determine the structure of the active compounds of these plants as well as to confirm the safety and efficacy of the extracts against disease conditions in livestock. Copyright / Urginea sanguinea, Aloe marlothii, Elephantorrhiza elephantina and Rhoicissus tridentata word tradisioneel gebruik vir die bekamping van siektes deur bosluise oorgedra in die Madikwe gebied van die Noordwes provinsie. Ekstrakte van hierdie species word gebruik teen “seme”, “gala” en “Bolwetsi jwa mothlapo o moshibidu” wat waarskynlik op hartwater, galsiekte and rooiwater dui volgens die beskikbare epidemiologiese data. Asetoon en metanol ekstrakte is gebruik vir die bepaling van antimikrobiese, antiparasitiese en antioksidant aktiwiteite in verskillende species deur gevalideerde in vitro metodes. Selkulture van Ehrlichia ruminantium, Babesia caballi en Theileria equi, , is in ‘n model gebruik om die doeltreffendheid van ekstrakte teen algemene siektes deur protozoa en ricketsias te bepaal. Vier algemene menslike nosokomiale patogene Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli, is gebruik om antibakteriese aktiwiteit van ekstrakte te bepaal. Difeniel-pikrielhidrasiel en die trolox ekwivalente anti-oksidant essajeermetode is gebruik om anti-oksidantaktiwiteit te bepaal. Antioksidante mag herstel na infeksies bespoedig deur stimulering van die immuunstelsel. Daar was ‘n groot verskil in die aktiwiteite tussen die verskillende ekstrakte en organismes. E. elephantina ekstrakte was die mees doeltreffende met die biologiese and chemiese bepalings. R. tridentata het sterk anti-oksidantaktiwiteit gehad, maar het lae aktiwiteit (> 100 µg/ml) teen bosluis-oorgedraagde parasiete gehad. U. sanguinea ekstrakte was aktief in beide die antibakteriese en anti-riketsiale bepalings (EC50 = 44.49 ng/ml), wat moontlik toegeskryf kan word aan die giftige bufadienoliede teenwoordig in hierdie species. A. marlothii ekstrakte het betekenisbolle anti-riketsiale aktiwiteit (EC50 = 111.4 µg/ml) maar slegs geringe antibakteriese aktiwiteit gehad. Hierdie resultate bevestig die moontlike waarde van hierdie species teen hartwater, galsiekte and rooiwater, en ondersteun die tradisionele etnoveterinêre gebruik teen “Seme, Gala, and Bolwetsi jwa mothlapo o moshibidu”. Verdere studies word benodig om die 5 aktiewe verbindings te isoleer en te karakteriseer en om die veiligheid en doeltreffendheid van ekstrakte teen hierdie siektes in vee te bevestig. / Dissertation (MSc (Veterinary Science))--University of Pretoria, 2004. / Paraclinical Sciences / unrestricted

This dissertation contributes to understanding the political behavior of two underrepresented groups: women and ethnoracial minorities. It explores how these groups' political behavior and beliefs are associated with existent gaps in representation. Each chapter approaches a different aspect concerning this common problem. Chapter 1 inquires about the mechanisms assumed to link descriptive and substantive representation for women. By analyzing the combination of electoral data segregated by gender in Ecuador with census data and the results of an original candidates' survey, I confirm the existence of a `gender affinity vote' and the importance of the type of female candidate for understanding gender gaps in support for women candidates. Chapter 2 presents the results of two survey experiments that study how gender stereotypes affect political behavior at the mass level. It identifies a gendered `issue ownership' based on these stereotypes and tests if counterstereotypical exposures promote more engagement of those underrepresented. The findings suggest that counterstereotypical exposure is not equally effective in promoting participation for both genders. Women do not get more engaged in male-dominated issues when encouraged by other women. Men get more engaged on women's issues when other men encourage them and when the invitee is similar to them. However, the interaction between the two factors has a negative effect, suggesting that seeing someone identical to them creates a dissonance that hinders the direct effects of the two variables. Chapter 3 presents the results of an audit study of US state legislators that explores the existence of a cominority solidarity between Blacks and Latinos. The results show that Latinos are not only the most disadvantaged because White legislators are biased against them, but also because their cominority solidarity towards Blacks is not reciprocated.

This thesis concerns the application of different artificial neural network architectures on the classification of multivariate accelerometer time series data into activity classes such as sitting, lying down, running, or walking. There is a strong correlation between increased health risks in children and their amount of daily screen time (as reported in questionnaires). The dependency is not clearly understood, as there are no such dependencies reported when the sedentary (idle) time is measured objectively. Consequently, there is an interest from the medical side to be able to perform such objective measurements. To enable large studies the measurement equipment should ideally be low-cost and non-intrusive. The report investigates how well these movement patterns can be distinguished given a certain measurement setup and a certain network structure, and how well the networks generalise to noisier data. Recurrent neural networks are given extra attention among the different networks, since they are considered well suited for data of sequential nature. Close to state-of-the-art results (95% weighted F1-score) are obtained for the tasks with 4 and 5 classes, which is notable since a considerably smaller number of sensors is used than in the previously published results. Another contribution of this thesis is that a new labeled dataset with 12 activity categories is provided, consisting of around 6 hours of recordings, comparable in number of samples to benchmarking datasets. The data collection was made in collaboration with the Department of Public Health at Karolinska Institutet. / Inom ramen för uppsatsen testas hur väl rörelsemönster kan urskiljas ur accelerometerdatamed hjälp av den gren av maskininlärning som kallas djupinlärning; där djupa artificiellaneurala nätverk av noder funktionsapproximerar mappandes från domänen av sensordatatill olika fördefinerade kategorier av aktiviteter så som gång, stående, sittande eller liggande.Det finns ett intresse från den medicinska sidan att kunna mäta fysisk aktivitet objektivt,bland annat eftersom det visats att det finns en korrelation mellan ökade hälsorisker hosbarn och deras mängd daglig skärmtid. Denna typ av mätningar ska helst kunna göras medicke-invasiv utrustning till låg kostnad för att kunna göra större studier.Enklare nätverksarkitekturer samt återimplementeringar av bästa möjliga teknik inomområdet Mänsklig aktivitetsigenkänning (HAR) testas både på ett benchmarkingdataset ochpå egeninhämtad data i samarbete med Institutet för Folkhälsovetenskap på Karolinska Institutetoch resultat redovisas för olika val av möjliga klassificeringar och olika antal dimensionerper mätpunkt. De uppnådda resultaten (95% F1-score) på ett 4- och 5-klass-problem ärjämförbara med de bästa tidigare publicerade resultaten för aktivitetsigenkänning, vilket äranmärkningsvärt då då betydligt färre accelerometrar har använts här än i de åsyftade studierna.Förutom klassificeringsresultaten som redovisas bidrar det här arbetet med ett nyttinhämtat och kategorimärkt dataset; KTH-KI-AA. Det är jämförbart i antal datapunkter medspridda benchmarkingdataset inom HAR-området.

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