A seliberia-like bacterium (SLO), isolated from reverse osmosis membranes was characterized by morphological, physiological and DNA studies. The helically twisted cells of this organism were often observed in star-shaped clusters. Depending on nutritional conditions, cells ranged from 0.5 to 21 um in length and possessed prosthecae. Small motile cells were produced by asymmetric fission or by a budding process. Ovoid "generative" cells were observed in mixed culture conditions or when the pure culture isolate was grown in the presence of humic acid. The SLO oxidatively utilized glucose, maltose, xylose, cellobiose, and several amino acids as sole carbon and energy sources. The organism is a strict aerobe and does not anaerobically respire. The moles percent guanine plus cytosine (mol% G + C) of the SLO DNA was 38% as compared with 63-67% for Seliberia stellata. Although the cellular morphology and physiology of the SLO closely resembles that of S. stellata, the SLO is considered to be a new species of Seliberia based on the presence of prosthecae and the mol% G + C.

Background: The high frequency of emergency responses and the variety of response environments for firefighting and emergency medical services dictate a potential for high work demands and an increasing need for maintaining above average physical fitness. This study makes use of a retrospective occupational cohort study design to explore the relationships between measures of fitness and injury outcomes. Methods: Data were collected from annual medical exams and injury surveillance records recorded for the years 2004-2009 among commissioned employees of the Tucson Fire Department. Fitness was assessed and contrasted via a submaximal estimate of aerobic capacity and a developed metric and score for comprehensive "fire fitness" encompassing seven separate measures for strength, endurance, flexibility, body composition and aerobic fitness. Individual fitness scores were classified as 'high fit', 'fit', and 'less fit'. The association between the fitness measures and injuries was evaluated using two approaches: log-binomial and time-to-event analyses. Results: The annual injury incidence rate averaged 17.7 per 100 employees. One-third of all injuries (32.9%) resulted from physical exercise activities, while patient transport, training drills, and fireground operations resulted in 16.9%, 11.1% and 10.2% of injuries, respectively. For all job operations, sprains and strains were the most prevalent type of injury, followed by contusions and lacerations. The reliability of fitness and clinical measures showed mean flexibility, grip strength, percent body fat, and resting heart rate each had intraclass correlations (ICC) values above 0.5, suggesting fair to good reliability. In contrast, mean VO2max was an unreliable measure with an ICC of 0.27. Hazard ratios from time-to-event analyses indicated that increases in cardiorespiratory fitness were significantly associated with decreased risk against injury. Similarly, decreases in comprehensive "fire fitness" were associated with an increased risk of injury. Conclusions: These findings add support that improving one's fitness reduces the likelihood of injury. Future research should focus on the relationship between fitness, performance and health outcomes. Individual level fitness improvements should be objectively measured and designed within the functional limitations of that individual, and without subjecting the person to injury in that process.

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The purpose of the study was to examine the relationship between treadmill time, maximal oxygen consumption and pace characteristics of the 1.5 and 3 mile runs and to compare the distances and pace characteristics as predictors of aerobic capacity. Subjects were 70 college aged males, ages 18 to 25, enrolled in jogging and conditioning classes at North Texas State University. Three tests were administered: the 1.5 mile run, the 3 mile run and the Bruce treadmill test. The data were analyzed using correlations and factor analysis. Conclusions of the investigation were (1) the 1.5 and 3 mile runs are valid measures of aerobic capacity, (2) the 3 mile run does not significantly increase the correlation between VO2max and endurance runs and (3) pacing characteristics are evident in the 1.5 and 3 mile runs.

Title: The most common aerobic injuries, their prevention and follow rehabilitation. Objective: The aim of this thesis is find and introduce main type of injuries, frequency and lenght of the recovery of aerobic competitors of I., II. and III. FISAF class. Asses injuries and find oue preventive measures used by competitors. Methods: In this diplome thesis was used literary review in theoretical part of thesis and metod of written questioning was used in non-standatdized questionaires. This method was used to analyse the most common injuries, preventive measures before injury and find out competitors experience with physical therapy The questionnaire was distributed to 21 aerobic clubs wich contest in 1st, 2nd and 3th aerobic FISAF class. For data analysis softwere was used Microsoft Excel for Windows 2013. Findings: Acquired data showed that in season of 2016 was injured 91 competitors of 157 participant researchers. The most common area of injury aerobics FISAF competitors of 1st, 2nd and 3th class was in 57% pelvis and lower extreminty area. Detailed analysis showed that 24% of injury was in the ankle joint. It was founded 56% injuries happend during training practitions. 74% competitors, said, that they are using preventive measures during practising. 65% of whole competetors has previous...

Student Number : 9902262G - MSc Dissertation - School of Molecular and Cell Biology - Faculty of Science / Anaerobic fermentative bacteria degrade waste components in landfills where methane (CH4) and carbon dioxide (CO2) are the primary biogases emitted and methanotrophic bacteria in the cover soil oxidise the emitted CH4. Three bi-phasic bench-scale landfill bioreactors were commissioned to evaluate soil nutrient addition effects on CH4 formation and oxidation and to isolate inherent soil methanotrophs using Nitrate Mineral Salts (NMS) medium. Set A soil contained no nutrient additions, Set B soil contained 50 μM nitrate and 150 μM phosphate and Set C soil contained dried sewage cake. Bioreactors were run for a 4 week period and pH, anaerobic gas emissions, volatile fatty acids (VFA), bacterial counts and scanning electron microscopy (SEM) analyses were performed. A pilot study revealed that pH dictated the stability of methanogenesis, where increased VFA levels inhibited methanogenesis. Furthermore, it was revealed that modifications of the NMS medium were needed to enrich for methanotrophs. An in depth study showed that the Set C anaerobic reactor produced the most methane with Set B the least. The hypothesis that methane oxidation in the soil could regulate methane formation in the waste could not be conclusively observed, as a lack of aeration in the soil reactors is believed to have prevented the proliferation of methanotrophs here. No methanotrophs were successfully isolated from soil, but rather major heterotrophic bacterial interference was observed. SEM revealed the presence of rod and cocci forms of bacteria in both leachate and soil, consistent with literature reports, which indicated that the bench-scale landfill bioreactors were capable of promoting bacterial growth.

Thesis (M.Sc.)--University of the Witwatersrand, Faculty of Science, 2013 / Unable to load abstract.

This research investigated the relationship between exercise training and cathepsin B expression in the 3xTg-AD murine model of Alzheimer’s disease. 3xTg-AD mice were assigned to control (Tg, n=10), aerobic training (Tg+AT, n=10), or resistance training (Tg+RT, n=10). RotaRod peak latency and grip strength were assessed as preand post-measurements. Skeletal muscle was collected after training and analyzed for cathepsin B protein. Tg+RT showed greater grip strength than Tg and Tg+AT at posttesting (p ≤ 0.05). Only Tg+AT showed an improvement in RotaRod peak latency (p ≤ 0.05). Gastrocnemius weight was greater in Tg+RT compared to Tg (p ≤ 0.05), and no differences were observed in cathepsin B or procathepsin B expression (p > 0.05). This data suggests that cathepsin B was not induced by either mode of exercise training, however, physical function and muscle mass were improved, therefore inclusion of both training modalities may address peripheral comorbidities in Alzheimer’s disease. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2018. / FAU Electronic Theses and Dissertations Collection

Due to the pressure of recent legislative changes (eg: the EU Landfill Directive and the imposition of a Landfill Tax), composting as a waste disposal technique is now being viewed as the preferred alternative to the landfilling of organic waste. However, while composting has been practised in one form or another for 2500 years, the underlying principles behind the microbial ecology of composting, is poorly understood. In order to obtain an insight into the ecology and hence, the critical nature of the composting process, a number of low cost open-windrows containing urban botanical wastes were established. These windrows were subjected to microbial and physico-chemical analysis over the initial period of exothermically active composting (25 days). This study demonstrated that, whilst average temperatures within open windrows can reach in excess of 65 °C, the sustainability and range of these temperatures depended upon the windrow bulk density. Windrows with bulk densities of 600kgm*3 had a larger insulation factor and thus, were able to sustain high temperatures for longer periods. However, these windrows were more susceptible to the development of areas of low temperature (cold spots) at depths below 20cm. Windrows with bulk densities of 400kgm'3 had smaller insulation factors and therefore, lost heat at a faster rate than windrows with higher bulk densities. This loss of heat was observed to be the case with the windrow surface layers, but they exhibited fewer cool spots at lower levels. This study found that the average microbial population of windrow material was 2.29x1013 CFU kg*1 and that each microbial cell could generate between 6.33 and 8.56xl0*13 Mjkg*1. This resulted in the generation of between 1.13 and 1.70 Mjkg*1 °C*1 of heat energy. Contrary to the published literature, this study observed that temperatures above 65 °C did not result in the significant loss of ammonia from the windrow. However, high levels of ammonia did suppress the formation of nitrate within the windrows. Experiments investigating microbial population kinetics within the windrows indicated that observed changes were proportional to temperature up to 60 °C, when a reduction in population numbers was observed between 60 °C and 65 °C. However, between 60 °C and 70 °C population levels increased once again. It was also noted that at the start of the composting process, 13 different microbial species or genera could be identified. However, after 17 days of exothermic composting, this had been reduced to 2 genera, including a novel large bacterial species belonging to the genus Bacillus. This study also showed that samples of windrows exposed to temperatures above 55 °C for 48 hours did not eliminate mesophilic or psycrotrophic microbial populations as previously assumed by other workers, but only suppressed their metabolism during the high temperature period.