No description available.

No description available.

<p>Coastal wetlands are hydrologically connected to their watershed and the lake. Water levels in Georgian Bay have been at a sustained low for thirteen years and thus connectivity of wetlands to the lake is being threatened as water levels decline. Decreased connectivity has likely caused changes in ecological and chemical characteristics. Future climate change models predict further water declines and potentially increasing the number of wetlands that will be hydrologically disconnected. The over-arching goal of this thesis is to investigate the role of connectivity between the lake and coastal marshes in eastern Georgian Bay on the amount of potential fish habitat, water chemistry and larval amphibian habitat.</p> <p>Bathymetric information is needed in order to estimate fish habitat and two approaches were utilized in order to collect these data. A site-specific method completed in 2009 used an intensive field survey in seven wetlands to create a digital elevation model and calculated the amount of fish habitat at 10 cm increments. A second, regional method, selected 103 sites by using a stratified random sample in 18 quaternary watersheds. In both methods, changes in water levels between 173 and 176 m asl resulted in the most drastic loss of habitat. Approximately 24% of the current fish habitat has already been lost due to low water levels.</p> <p>Water chemistry in coastal marshes is influenced by hydrologic connection. In the summers of 2010 and 2011, 35 coastal marshes were sampled, 17 of which had been impounded and disconnected by a beaver dam. Beaver-impounded marshes resulted in significantly lower pH, conductivity, dissolved oxygen and sulphate concentrations, but had significantly higher soluble reactive phosphors concentrations. These conditions are indicative of the lack of connection and reduced mixing with lake water. This altered habitat was shown to support breeding area for 7 species of amphibians, the most common being green frogs and the least common being American Toads and chorus frogs.</p> / Master of Science (MSc)

Biodiversity is a complex concept encapsulating the variation that occurs within and among levels of biological organization. It is positively linked to ecosystem persistence, adaptability, and function. Biodiversity loss, driven by global change and human activities, is one of the most prominent threats to ecosystems. Characterizing the variation of and processes driving biodiversity is a critical step in understanding the causes, consequences, and magnitude of biodiversity loss. However, characterizing biodiversity comprehensively requires understanding multiple dimensions, or types, of diversity, such as genetic, taxonomic, phylogenetic, and life history diversity, that encompass both ecological and evolutionary processes varying across space and time. In this dissertation, I investigate spatial and temporal variation in frog and toad (order Anura) biodiversity to understand the effects of how diversity is measured on biodiversity characterization and the underlying processes driving biodiversity. In my first chapter, I examined the spatial and temporal variation of genetic diversity and other population genetic metrics to understand the effects of multi-year sampling on population genetic inference in an anuran metapopulation (Arizona treefrog, Hyla (Dryophytes) wrightorum). I found that a single sample year captures global, but not local, population genetic dynamics, as there is considerable temporal variation in genetic metrics within individual populations. In my second chapter, I developed a tool to improve the characterization of anuran life history diversity using species traits. Traits are the measurable attributes of species, and a suite of species traits is used to distinguish ecological strategies found among species. I collated trait data from 411 primary and secondary sources for 106 anuran species found in the United States to develop an anuran traits database for use in conservation, management, and research. In my third chapter, I investigated spatial variation within and among taxonomic, phylogenetic, and life history anuran diversity in the United States and examined the abiotic relationships behind observed patterns. To do this, I developed species distribution models at a 1 km2 resolution for the majority of the native US anurans. I identified relationships among diversity metrics for improved, comprehensive biodiversity characterization and potential ecological and evolutionary processes underlying biodiversity. Spatial variation in multidimensional relationships highlights regional needs for multiple metrics of diversity to comprehensively characterize biodiversity. This spatial variation is driven by temperature, elevation, and water availability, likely related to the biological limits for anurans. Collectively, these chapters highlight the considerable variation that exists within and among species of a broad and diverse biological. Furthermore, these chapters call attention to the importance of measuring multiple biodiversity dimensions for effective conservation in a rapidly changing world. / Doctor of Philosophy / Biodiversity loss, from global change and human activities, is one of the biggest threats to the Earth's ecosystems. Biodiversity is the similarities and differences in organismal characteristics, such as their genetics, evolutionary history, and ecology. Biodiversity is often linked to how well an ecosystem will persist and adapt to global change. To understand the causes and consequences of biodiversity loss, it is important to first measure diversity and what shapes it. However, there are many types of diversity and ways to measure them, such as the number of species in a system, or species richness, the span of evolutionary lineages in a system, or phylogenetic diversity, the number of roles species fill in a system, or life history diversity, and the genetic relationships within individuals and populations, or genetic diversity. In this dissertation, I investigate how our understanding of biodiversity is affected by the ways it can be measured and the relationship among those metrics. To do this, I studied diversity in three ways for frogs and toads of the United States. First, I investigated whether sampling across multiple years is necessary to adequately characterize genetic diversity in a frog species (Arizona treefrog: Hyla (Dryophytes) wrightorum) with populations that fluctuate in size over time. Here, I found that multiple sample years are necessary to capture the genetic variation within individual populations over time, but unnecessary to capture the average genetic variation among all populations over space and time. Second, I developed a tool to improve our ability to measure anuran life history diversity using traits. Traits are measurable attributes of species, and multiple species traits can be used to define the role of a species in an ecosystem. I collected trait data from 411 sources for 106 frog and toad species found in the United States to develop a traits database for use in conservation, management, and research. Third, I investigated the similarities in multiple diversity metrics across the United States using species richness, phylogenetic diversity, and life history diversity. Species richness is highest in the eastern US, phylogenetic diversity is highest in the western US, and life history diversity is clustered around eastern US mountains. These regional relationships among metrics coincided with regional water availability, temperature, and elevation. These results collectively call attention to changes over space and time in frog and toad diversity and how the relationships within and among diversity types relate to our understanding of frog and toad biodiversity. Considering how, where, and when to measure biodiversity can lead to more effect biodiversity conservation in a rapidly changing world.

Amphibians, because of their semi-permeable skin, sensitivity to changing microclimates, and important role in ecosystems, are often viewed as indicators of ecosystem health. They make excellent organisms for studies on the effects of silvicultural practices. My goal was to provide recommendations for forest management in the southern Appalachians so that harvesting operations are compatible with maintaining healthy populations of forest amphibians. I tested previously created habitat models that determined the most important habitat characteristics for salamanders. I counted salamanders in 240 10 x 10 m plots located in the MeadWestvaco Wildlife and Ecosystem Research Forest in north-central West Virginia. We also collected a variety of habitat data in these plots to predict salamander abundance with previously created models. These simple linear regression analyses of predicted versus observed values suggest for most models (7 out of 9) a weak relationship between predicted and observed values (R2 from 0.0033 to 0.2869, p from < 0.0001 to 0.7490). However, one of the models showed characteristics suggesting that it predicted new data as well or better than the original data, and therefore was the most accurate at predicting salamander abundance, and could be used for management purposes, although there was still much unexplained variation. This model included the variables woody stems (< 7.5 cm DBH), available rock, riparian status (i.e., within 15 m of a stream), percent overstory canopy cover, and available highly decomposed woody debris (decomposition classes 3 to 5). All of these relationships were positive except for woody stems, suggesting that in order to maintain healthy populations of salamanders, we should protect areas next to streams, with high amounts of rock, decomposed woody debris, overstory canopy cover, and few woody stems. I also examined the immediate effects of clearcuts on salamander movement and population estimates. I batch marked salamanders in plots at the edges of a clearcut, and in a control plot. Using the Schnabel estimator, I estimated population sizes in each plot. I then compared population estimates pre- and post-harvest on the interior (harvested) and exterior (unharvested) sides of the plots, taking into account the control plot. I also examined adult-juvenile ratios and movements from one side of the plot to the other. I found no significant changes (p > 0.05) following harvest in any of these measures, suggesting that salamanders do not move out of the harvested area post-harvest, at least over the short term (10 months of this study). This suggests that a longer period of time (> 1 year) is required to observe the population declines detected in most studies. / Master of Science

Coal mining has had profound impacts in the Appalachian region, initiating a need to understand the implications of traditional and current reclamation practices on wildlife. I evaluated wildlife use of reclaimed sites of varying ages and cover types in southwestern Virginia. I compared reclaimed sites to another form of anthropogenic disturbance (clearcut) and relatively undisturbed mature forest. Birds were surveyed during early mornings throughout the breeding season in 2007 and 2008 using the point count method. Amphibians were surveyed using artificial cover, constrained-time night searches, and auditory pond surveys. Microhabitat data were collected at each sampling point and were combined with landscape-level GIS information to relate habitat characteristics and wildlife patterns. I observed 80 bird species using reclaimed areas, clearcuts, and mature forest. Pre-regulation sites (prior to the Surface Mining Control and Reclamation Act of 1977) supported the highest number of species overall. Cluster analysis identified 4 bird associations based on habitat characteristics. I developed site-specific, landscape-level, and mixed-scale logistic regression models to identify habitat characteristics that best predicted the presence of 27 species. For 18 species, mixed-scale models performed best, suggesting the importance of a multi-scale approach to habitat analysis. Salamanders were generally not detected on reclaimed areas, possibly due to the lack of soil moisture, leaf litter, and woody debris on young sites. Frogs were present in all water bodies surveyed, suggesting the importance of managing ponds and wetlands on reclaimed sites. Identifying and focusing on important habitat characteristics will help managers enhance post-mining land for wildlife. / Master of Science

Globally, as many as 30% of amphibians and 23% of reptile species are threatened and require urgent conservation action. The major threats are primarily caused by anthropogenic activities. This research aimed to investigate the impacts of two anthropogenic threats, over-exploitation and habitat changes on herpetofaunal populations in Hong Kong. Platysternon megacephalum has been heavily depleted because of severe hunting pressure for the food and pet trades. Low densities of individuals and few remaining intact populations have limited our ability to study their ecology. I conducted mark-recapture and radio-telemetry studies on P.megacephalum populations in five streams in Hong Kong, one of which was free from trapping, over 34 months (2009–2011) to investigate the impacts of illegal trapping on populations of P.megacephalum and their spatial ecology and growth. Illegal trapping was associated with the absence of large adults, smaller body sizes of adults and skewed ratios of juveniles to adults. Home ranges were relatively small with a mean 100% minimum convex polygon 996m2. Males moved longer distances than females and both sexes moved longer distances in wet seasons. P.megacephalum was highly aquatic, preferring to stay in pools, and their microhabitat preferences were affected by stream width and depth, and substrate types. Juvenile P.megacephalum grew rapidly, with growth declining after attainment of sexual maturity. The average ages of sexual maturation were eight years for females. Illegal trapping remains the major threat to P.megacephalum populations and I recommend that regulatory personnel identify key streams and patrol regularly against illegal trapping to safeguard remaining populations. In Hong Kong, large areas of primary forest have been replaced by secondary forests and plantations, and changes in tree species composition have often led to alteration of associated plant and animal communities. I examined the herpetofaunal assemblages in secondary forests and exotic Lophostemon confertus plantations. Amphibian abundances were higher in secondary forests while reptile abundance, species richness of amphibian and reptiles were similar. Secondary forests provided better habitats for amphibians and I recommend the planting of a mixture of native tree species and the thinning of exotic trees in future plantation management efforts in South China. Our knowledge about the effectiveness of different herpetofaunal survey methods in Southeast Asia is limited. To fill the information gap, I examined the effectiveness of three survey methods, including transect surveys, pitfall traps and coverboards, for sampling terrestrial herpetofauna. Transect surveys were most effective at sampling species richness and pitfall traps were most efficient in capturing high numbers of reptiles. The results of this study will aid researchers in assessing the feasibility of and choosing herpetofaunal survey methods in Southeast Asia. Despite the severe threats that herpetofauna are facing, our understanding of their ecology and conservation needs remains limited. More research and the initiation of monitoring programs for herpetofauna, strengthened enforcement of existing regulations, and proper habitat management are crucial for the conservation of herpetofauna in South China. / published_or_final_version / Biological Sciences / Doctoral / Doctor of Philosophy

Changes of habitats are amongst the main drivers of evolutionary processes. Corresponding shifts in the behaviour and life history traits of species might in turn also alter ecosystem attributes. The reproduction of Western European fire salamanders (Salamandra salamandra), in small pond habitats instead of first order streams, is one example of a recent local adaptation. Since fire salamander larvae are important top-predators in these fish free habitats, their presence likely changes various aspects of ecosystem functioning. Here, it was analysed how the ecological performance of salamander larvae in ponds in the Kottenforst in Western Germany changed in comparison to sympatric stream populations. Further, it was analysed how their presence in ponds influenced key ecosystem attributes such as prey density and diversity and aquatic-terrestrial linkage. To assess the impact of the life cycle shifts in salamanders on the pond functioning, detailed investigations of salamander larvae population dynamics, phenology, and macroinvertebrate community development in ponds were combined with experimental manipulations of the salamander presence. In the first part of this study, the impact of pond presence of fire salamanders in terms of ecosystem functioning focussing on aquatic terrestrial subsidy transfer was calculated. The study could show, that the adaptation of fire salamanders to breed in pools led to strong increases of animal-mediated import of terrestrial matter into the aquatic habitats. The hypothesis about the impact on macroinvertebrate communities derived from these calculations was then tested experimentally. It was shown, that presence of salamander larvae could influence some taxa of macroinvertebrates but they had only limited effects on the food web structure in their aquatic habitats. Yet, a high relevance of the subsidy exchange from aquatic to terrestrial and its high relevance for the predator persistence in the system could again be confirmed. Moreover, it was demonstrated, that the larval behaviour and performance could have a high inter-annual variability as a reaction to contrasting ecosystem constraints in comparison to the stream habitats. A fact that integrally separates the pond ecotype from stream ecotype conspecifics.

In this study, I used restriction fragment length polymorphism (RFLP) analysis (DNA fingerprinting) to study the genetic population structure of wood frogs, Rana sylvatica, collected as egg masses from vernal pools within the Massachusetts Audubon Society Lincoln Woods Wildlife Sanctuary in Leominster, MA. The average genetic relatedness of sibling individuals, non-sibling individuals from within the same pool, and individuals from pools of close (5 m), far (200 m) and distant (40 km) spatial separations was calculated. The goal was to use genetic relatedness to estimate the breeding patterns of R. sylvatica and use that information to make general management recommendations that could be applied to other vernal pools breeders. I detected relative differences in genetic similarity between individuals from pools only 5 meters apart, however over a larger distance of 200 meters no significant genetic differences were present. This suggests that although wood frogs are known to be highly philopatric, they may use information other than simply proximity to their natal pool when choosing breeding sites. Factors such as species composition, water chemistry and heterogeneity of the landscape between pools may influence breeding site choice. Also, contrary to the findings of recent studies, the distance between vernal pools may not be the best indicator of the genetic similarity of the individuals they host. Pools in close proximity to one another may host genetically distinct populations, and therefore management decisions should be made on a pool-by-pool basis. Consequently, when managing populations of R. sylvatica, and possibly other vernal pool breeders, taking into account parameters other than simply the spatial separation of pools within an array may avoid decisions that could result in the loss of genetic diversity.

Rocky plateaus are globally threatened ecosystems and the lateritic plateaus in northern section of the Indian Western Ghats/Sri Lanka Biodiversity Hotspot (NWG) are notable landscape features. The NWG are geologically distinct from and biologically isolated from the other two sections of the Western Ghats. The NWG is known to possess elevated levels of endemic flora their biotic relationships remain poorly understood. We present here the first quantitative multi-taxa comparative study of plateau fauna in the northern Western Ghats. Ants, water beetles and amphibians were selected for the comparison as they use different ecological resources from each other and at various times of year therefore may provide a wider representation of plateau usage. Distribution and endemism were expected to reflect distribution and isolation by plateau and inter-plateau environment related to elevational, latitudinal, climatic and land-use clines. The study explored patterns of amphibian, water beetle, ant and fungal distribution and sampling seasonal pools and terrestrial surfaces of 13 representative plateaus in western Maharashtra, with sites ranging from 67-1179 m and across 2° of (sub) tropical latitude. Distribution of all taxonomic groups was spatially non-random at all levels of organisation and across all spatial scales. At the macroscale assemblages differed significantly with climate related to elevation, latitude but at a local scale land-use and microhabitat availably had an impact. Ants displayed a large seasonal assemblage variation, responding to seed availability. Water beetles the ants (combined survey data) were the best surrogates for all taxa data with amphibians the weakest representatives. Infection by the fungal pathogen, Batrachochytrium dendrobatidis, was extensive but less intense below the escarpment. We report the first records of infection in 13 endangered and data deficient amphibian species in the Western Ghats. The plateaus of western Maharashtra have recently been recognised as fragile and threatened ecosystems most still lack adequate statutory protection. In the absence of peer reviewed comparative data, the importance of individual sites is difficult to demonstrate hampering evidence-based conservation decisions.