Made available in DSpace on 2014-12-17T14:10:26Z (GMT). No. of bitstreams: 1 TiaraSC_DISSERT_PARCIAL.pdf: 3904486 bytes, checksum: c7dbd69e3fa57b6310c7eaf6781c6f83 (MD5) Previous issue date: 2011-07-15 / Gasteroids fungi are characterized by the basidiospores maturation inside the basidioma, from which spores liberation occurs in a passive manner. These fungi were once seen as a well definite class of Basidiomycota, but nowadays they are considered an artificial assemblage, because the organisms have independent evolutionary histories forming a polyphyletic group with a vast morphological variety. Despite their diversity, studies with this group in the tropics are incipient, and the phylogenetic relationships of the species from temperate climate remain unknown. Thus, this work aimed to elucidate the phylogenetic relationships of gasteroids fungi from the Geastrales and Phallales orders, with the inclusion of tropical and temperate species, and with these analyses suggest a systematic position of species like Asero? floriformis and Phallus roseus, as well as to verify if the lignicolous habit can indicate parental relationship in the Geastrum genus. For this, basidiomata were collected at Atlantic rain forest areas, during the rainy season, and the specimen identification followed specific literature for gasteroid fungi. The phylogenetic analyses were performed with Maximum Parsimony and Bayesian Analysis, making use of RPB2 and 28S nuclear genes and atp6 mitochondrial gene. It could be observed on the Phallales dendogram, that Asero? floriformis did not cluster with A. rubra, and that it has an anterior divergence from all others species of the family Clathraceae used in this analysis, assuming a basal position in the clade. Phallus roseus, which once was recognized as Itajahya, has previous divergence from the group formed by Phallus species. At the Geastrales dendogram, in the group corresponding to Geastrum genus, it could be observed that species with lignicolous habitat clustered in a clade with high support values. So, the results suggest the creation of a new genus to accommodate A. floriformis, and the revalidation of Itajahya, as well as it can be affirmed that the lignicolous habitat on the Geastrum genus in fact indicates parental relationships, and that it has arised only once at the evolutionary history of the genus / Os fungos gasteroides s?o reconhecidos pela matura??o dos basidi?sporos dentro do basidioma, cuja libera??o ocorre de forma passiva. Esses fungos j? foram vistos como uma classe bem definida de Basidiomycota, mas atualmente s?o considerados um grupo artificial, por tratar-se de organismos com hist?rias evolutivas independentes formando um grupo polifil?tico com grande diversidade morfol?gica. Apesar da grande diversidade, estudos com esse grupo nos tr?picos ainda ? incipiente, e as rela??es filogen?ticas com esp?cies de regi?es temperadas permanecem desconhecidas. Dessa forma, objetivou-se neste trabalho elucidar as rela??es filogen?ticas de fungos gasteroides das ordens Geastrales e Phallales, com a inclus?o de esp?cies tropicais e de regi?es temperadas, e atrav?s dessas an?lises conhecer a rela??o de Asero? floriformis e Phallus roseus com outros membros do grupo, assim como verificar se o h?bito lign?cola em Geastrum pode indicar rela??o de parentesco. Para isso, coletas de basidiomas foram realizadas em ?reas de Mata Atl?ntica em per?odo chuvoso, com a identifica??o dos esp?cimes seguindo bibliografia espec?fica para fungos gasteroides. Para as an?lises filogen?ticas foram utilizados os m?todos de M?xima Parcim?nia e An?lise Bayesiana, utilizando-se os genes nucleares RPB2 e 28S, e o gene mitocondrial atp6. Observou-se no dendograma obtido para a ordem Phallales, que Asero? floriformis n?o se agrupou com A. rubra, apresentando diverg?ncia anterior a todas as esp?cies da fam?lia Clathraceae utilizadas na an?lise, assumindo uma posi??o basal no clado. J? Phallus roseus, antes reconhecido como Itajahya, possui diverg?ncia anterior ao grupo formado pelas esp?cies de Phallus. No dendograma da ordem Geastrales, no grupamento correspondente ao g?nero Geastrum, p?de-se observar esp?cies que possuem h?bito lign?cola se agrupando com alto valor de suporte. Assim, os resultados sugerem a cria??o de um novo g?nero para acomodar A. floriformis, e a revalida??o do g?nero Itajahya, assim como se pode afirmar que o h?bito lign?cola em Geastrum de fato indica rela??es de parentesco, aparentemente tendo surgindo apenas uma vez na hist?ria evolutiva do grupo

Submitted by PPG Zoologia (zoologia-pg@pucrs.br) on 2018-05-18T17:22:44Z No. of bitstreams: 1 dissertacao_mestrado_final_paulochaves.pdf: 4426171 bytes, checksum: 8be6ef944f497d1a9518754ebfbc27c1 (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-05-28T12:19:27Z (GMT) No. of bitstreams: 1 dissertacao_mestrado_final_paulochaves.pdf: 4426171 bytes, checksum: 8be6ef944f497d1a9518754ebfbc27c1 (MD5) / Made available in DSpace on 2018-05-28T12:35:54Z (GMT). No. of bitstreams: 1 dissertacao_mestrado_final_paulochaves.pdf: 4426171 bytes, checksum: 8be6ef944f497d1a9518754ebfbc27c1 (MD5) Previous issue date: 2008-03-20 / Sequ?ncias de DNA usadas na identifica??o de material biol?gico t?m alcan?ado consider?vel popularidade nos ?ltimos anos, especialmente no contexto dos c?digos de barras de DNA. Aferir a esp?cie de origem em amostras de pelos, penas, peles e particularmente fezes ? um passo fundamental para quem estuda a ecologia e evolu??o de diversos animais com este tipo de amostra. Este ? o caso em carn?voros, cujos h?bitos furtivos e baixas densidades populacionais de algumas esp?cies evidenciam a import?ncia de estudos baseados em amostras n?o-invasivas. Entretanto a atual escassez de ensaios padronizados de identifica??o de carn?voros freq?entemente dificulta a aplica??o dessas amostras em larga escala e compara??es de resultados entre diferentes localidades. No presente estudo n?s avaliamos dois segmentos curtos (<250 pb) de DNA mitochondrial (mtDNA) localizados nos genes ATP sintase 6 e citocromo oxidase I com potencial de servirem como marcadores-padr?o para identifica??o de carn?voros. Entre um e 11 indiv?duos de 66 esp?cies de carn?voros foram seq?enciados para um ou ambos os segmentos do mtDNA e analisados usando tr?s diferentes m?todos (?rvore de dist?ncia, dist?ncia gen?tica e an?lise de caracteres). Em geral, indiv?duos conspec?ficos apresentaram menor dist?ncia gen?tica entre si do que em rela??o a outras esp?cies, formando agrupamentos monofil?ticos. Exce??es foram algumas esp?cies que divergiram recentemente, algumas das quais ainda puderam ser identificadas pelo m?todo de caracteres, hapl?tipos esp?cie-espec?ficos, ou reduzindo a abrang?ncia geogr?fica das compara??es (restringindo a an?lise a uma regi?o zoogeogr?fica). An?lises in silico, usando um segmento curto do citocromo b freq?entemente empregado em carn?voros, tamb?m foram realizadas para comparar o desempenho deste segmento em rela??o aos outros dois propostos. N?s ent?o testamos o desempenho destes segmentos na identifica??o de fezes de carn?voros por meio de tr?s estudos de caso: (i) fezes de felinos de zool?gico, objetivando-se verificar o potencial de contamina??o das seq?encias com DNA da presa (coelho); (ii) fezes coletadas no Cerrado brasileiro contendo restos de presas (p?los, ossos, penas), supostamente proveniente de lobo-guar?, objetivando-se investigar a efici?ncia de identifica??o do predador e ocorr?ncia de interfer?ncia do DNA da presa na identifica??o; e (iii) fezes coletadas em uma reserva na Mata Atl?ntica, tamb?m com o objetivo de avaliar a efici?ncia de identifica??o. Apesar de diferen?as em alguns aspectos de sua performance, nossos resultados indicam que os dois segmentos propostos t?m um bom potencial de servir como marcadores moleculares eficientes para identifica??o acurada de amostras de carn?voros ao n?vel de esp?cie. / DNA sequences for species-level identification of biological materials have achieved considerable popularity in the last few years, especially in the context of the DNA barcoding initiative. Species assignment of biological samples such as hairs, feathers, pelts and particularly faeces is a crucial step for those interested in studying ecology and evolution of many species with these samples. This is especially the case for carnivores, whose elusive habits and low densities highlight the importance of studies based on noninvasive samples. However, the current lack of standardized assays for carnivore identification often poses challenges to the large-scale application of this approach, as well as the cross-comparison of results among sites. Here we evaluate the potential of two short (<250 pb) mitochondrial DNA (mtDNA) segments located within the genes ATP synthase 6 and cytochrome oxidase I as standardized markers for carnivore identification. Between one and eleven individuals of 66 carnivore species were sequenced for one or both of these mtDNA segments and analyzed using three different approaches (tree-based, distance-based and character-based), in conjunction with sequences retrieved from public databases. In most cases, conspecific individuals had lower genetic distances from each other relative to other species, resulting in diagnosable monophyletic clusters. Notable exceptions were the more recently diverged species, some of which could still be identified using diagnostic character attributes, species-specific haplotypes, or by reducing the geographic scope of the comparison (restricting the analysis to a single zoogeographic region). Additional in silico analyses using a short cytochrome b segment frequently employed in carnivore identification were also performed aiming to compare performance to that of our two focal markers. We then tested the performance of these segments in the identification of carnivore faeces via three case studies: (i) felid faeces collected in a controlled zoo experiment, aimed at assessing whether DNA from rabbit prey would contaminate the resulting sequences; (ii) field-collected faeces from the Brazilian Cerrado presumed to be from maned wolves and containing prey remains (hairs, bones, feathers), aimed at investigating the efficiency of predator identification and occurrence of prey DNA interference; and (iii) field-collected scats from an Atlantic Forest study site, also addressing the issue of PCR success rate and identification efficiency. In spite of some relevant differences in some aspects of their performance, our results indicate that both of our focal segments have a good potential to serve as efficient molecular markers for accurate species-level identification of carnivore samples.

Mitochondrial diseases encompass a broad range of devastating disorders that typically affect tissues with high-energy requirements. These disorders have been difficult to diagnose and research because of the complexity of mitochondrial genetics, and the large variability seen among patient populations. We have devised and carried out a mechanistic study to generate a cell based model for Leigh’s disease caused by mitochondrial DNA mutation 8993 T>G. Leigh’s disease is a multi-organ system disorder that depends heavily on the mutation burden seen within various tissues. Using new reprogramming and sequencing technologies, we were able to show that Leigh’s disease patient fibroblasts reprogrammed to induced pluripotent stem cells maintain the same level of mutation burden seen in the original patient cell line. Mutation burden was maintained through several passages and spontaneous differentiation. This cell based model could be useful for future pathogenesis studies, or therapeutic drug screenings in a patient and tissue specific manner.

Disorders of ATP synthase, the key enzyme of mitochondrial energy provision belong to the most severe metabolic diseases presenting mostly as early-onset mitochondrial encephalo-cardio-myopathies. Mutations in four nuclear genes can result in isolated deficiency of ATP synthase, all sharing a similar biochemical phenotype - pronounced decrease in the content of fully assembled and functional ATP synthase complex. The thesis summarises studies on two distinct causes of ATP synthase deficiency. First is TMEM70 protein, a novel ancillary factor of ATP synthase, which represents most frequent determinant of severe inborn deficiency of ATP synthase. TMEM70 is a 21 kDa protein of the inner mitochondrial membrane, facilitating the biogenesis of mitochondrial ATP synthase, possibly through TMEM70 protein region exposed to the mitochondrial matrix, but the proper regulatory mechanism remains to be elucidated. In TMEM70-lacking patient fibroblasts the low content of ATP synthase induces compensatory adaptive upregulation of mitochondrial respiratory chain complexes III and IV, interestingly by a posttranscriptional mechanisms. The second type of ATP synthase deficiency studied was mtDNA m.9205delTA mutation affecting maturation of MT-ATP8/MT-ATP6/MT-CO3 mRNA and thus biosynthesis of Atp6 (subunit a) and Cox3...

Disorders of ATP synthase, the key enzyme of mitochondrial energy provision belong to the most severe metabolic diseases presenting mostly as early-onset mitochondrial encephalo-cardio-myopathies. Mutations in four nuclear genes can result in isolated deficiency of ATP synthase, all sharing a similar biochemical phenotype - pronounced decrease in the content of fully assembled and functional ATP synthase complex. The thesis summarises studies on two distinct causes of ATP synthase deficiency. First is TMEM70 protein, a novel ancillary factor of ATP synthase, which represents most frequent determinant of severe inborn deficiency of ATP synthase. TMEM70 is a 21 kDa protein of the inner mitochondrial membrane, facilitating the biogenesis of mitochondrial ATP synthase, possibly through TMEM70 protein region exposed to the mitochondrial matrix, but the proper regulatory mechanism remains to be elucidated. In TMEM70-lacking patient fibroblasts the low content of ATP synthase induces compensatory adaptive upregulation of mitochondrial respiratory chain complexes III and IV, interestingly by a posttranscriptional mechanisms. The second type of ATP synthase deficiency studied was mtDNA m.9205delTA mutation affecting maturation of MT-ATP8/MT-ATP6/MT-CO3 mRNA and thus biosynthesis of Atp6 (subunit a) and Cox3...