Communication via fiber optical cable (links) is the latest advancement of the transmission media currently used for data transmission. Despite initial problems, using of this medium in recent years has greatly expanded due to their indisputable advantages. Optical cable is a great combination of reliability and security of data transfer. Its constantly improving transmission parameters contribute to the high speeds in the long-distance transmission of data. In the theoretical part of this work some kinds of optical cables are mentioned, also the most important parameters and the most widely used methods of measurement of these cables. Apart from that other methods of joining optical cables and the most used types of optical cables are also mentioned. The values of the decline and reflection, which are led into the device after connecting these parts into the optical lines. The device using the measuring method of the reverse reflection is also described in this thesis altogether with its most important parts and the way of the display of the measured track on the graphic image of the device. The problematics of identification and decline dead zone and the factors influencing the lenght of dead zone – the ability to identify two independent events on the link patchcord of the variable lenght, is also described in details in this thesis. In the practical part a series of measurements and evaluation (numerical and graphical) of the measured values of decline lenghts and identificating dead zones of the OTDR device will be carried out.

Tato dizertace je zaměřena na medicínskou zobrazovací modalitu – ultrazvukovou počítačovou tomografii – a algoritmy zlepšující kvalitu zobrazení, zejména kalibraci USCT přístroje. USCT je novou modalitou kombinující ultrazvukový přenos signálů a principy tomografické rekonstrukce obrazů vyvíjených pro jiné tomografické systémy. V principu lze vytvořit kvantitativní 3D obrazové objemy s vysokým rozlišením a kontrastem. USCT je primárně určeno pro diagnózu rakoviny prsu. Autor spolupracoval na projektu Institutu Zpracování dat a Elektroniky, Forschungszentrum Karlsruhe, kde je USCT systém vyvíjen. Jeden ze zásadních problémů prototypu USCT v Karlsruhe byla absence kalibrace. Tisíce ultrazvukových měničů se liší v citlivosti, směrovosti a frekvenční odezvě. Tyto parametry jsou navíc proměnné v čase. Další a mnohem závažnější problém byl v pozičních odchylkách jednotlivých měničů. Všechny tyto aspekty mají vliv na konečnou kvalitu rekonstruovaných obrazů. Problém kalibrace si autor zvolil jako hlavní téma dizertace. Tato dizertace popisuje nové metody v oblastech rekonstrukce útlumových obrazů, kalibrace citlivosti měničů a zejména geometrická kalibrace pozic měničů. Tyto metody byly implementovány a otestovány na reálných datech pocházejících z prototypu USCT z Karlsruhe.

This diploma thesis deals with the study of possible interactions between hyaluronan (HA) and plasmid DNA (pDNA). Plasmid DNA was isolated from E. coli JM109 (pUC19) and resuspended in TE buffer as well as high molecular weight hyaluronan. Individual samples of pDNA, HA and pDNA-HA were characterized by gel electrophoresis, CD spectroscopy and high resolution ultrasonic spectroscopy. Agarose gel electrophoresis examined the effect of the addition of hyaluronan to plasmid DNA on the migration of samples to the positive electrode. Structural changes in pDNA-HA samples were examined using CD spectroscopy. Individual CD spectra describes the dependence of the difference in absorption coefficients for left-hand and right-handed elliptic polarized light at wavelength. High resolution ultrasonic spectroscopy has been used to study interactions. It is an analytical method based on ultrasonic velocity and attenuation. We classify this technique as a non-destructive method because the passing waves do not affect the structure of the analyzed sample.

To compare ultrasound-based acoustic structure quantification (ASQ) with established non-invasive techniques for grading and staging fatty liver disease.

In this study Monte Carlo solutions to the radiative transfer equations are used to model translational and rotational motion seismogram envelopes in random elastic media with deterministic background structure assuming multiple anisotropic scattering. The results of the Monte Carlo radiative transfer theory simulations are verified by comparisons with 3D full wave field finite difference simulations. The observation and modeling of the three additional components of rotational ground motions can provide independent information about seismic wave propagation in the Earth’s structure. Rotational motions around the vertical axis observed in the P-wave coda are of particular interest as they can only be excited by horizontally polarized shear waves and therefore indicate the conversion from P- to SH-energy by multiple scattering at 3D-heterogeneities. Scattering and attenuation parameters in south-east Germany beneath the Gräfenberg array and in the Vogtland region are estimated by comparisons of synthesized multi-component seismogram envelopes to seismic data from local and regional swarm earthquakes and to teleseismic events. In a first step, frequency dependent scattering and attenuation parameters from a local data set are estimated for the Vogtland region using nearby swarm earthquakes. The results from the elastic simulations are compared to outcomes from acoustic radiative transfer simulations. Both methods yield similar results and suggest that intrinsic attenuation dominates scattering attenuation. From the elastic simulations it is observable, that forward scattering is required to explain the data. However, the amount of forward scattering strength remains unresolvable. In a second step scattering and attenuation parameters beneath the Gräfenberg array are estimated using a nonlinear genetic inversion of seismogram envelopes from regional events at high frequencies (4–8 Hz). The preferred model of crustal heterogeneity consists of a random medium described by an exponential auto correlation function with a transport mean free path length of ∼ 420 km. The quality factor for elastic S-wave attenuation Q iS is around 700. In a final step simulations of teleseismic P-wave arrivals, using this estimated set of scattering and attenuation parameters, are compared to observed seismogram envelopes from deep events. Simulations of teleseismic events with the parameters found from the regional inversion show good agreement with the measured seismogram envelopes. This includes ringlaser observations of vertical rotations in the teleseismic P-wave coda that naturally result from the proposed model of wave scattering. The model also predicts, that the elastic energy recorded in the teleseismic P-coda is not equipartitioned, unlike the coda of regional events, but contains an excess of shear energy. The combined results from the three different data sets suggest that scattering generating the seismic coda mainly occurs in the crustal part of the lithosphere beneath the receivers. The observations do not require scattering of high frequency waves in the mantle, but weak scattering in the lithospheric mantle cannot be ruled out. / In dieser Studie werden Monte Carlo Lösungen für die Energietransfergleichungen genutzt, um Seismogrammeinhüllende von Translations- und Rotationsbewegungen zu modellieren. Die Ergebnisse der Monte Carlo Simulationen werden durch einen Vergleich mit 3D finiten Differenzen Simulationen verifiziert. Diese Modellierung findet in einem elastischen Zufallsmedium mit deterministischer Hintergrundstruktur unter Annahme multipler anisotroper Streuung statt. Die Beobachtung und Modellierung der drei zusätzlichen Komponenten der Rotationsbodenbewegungen kann unabhängige Informationen über die Ausbreitung seismischer Wellen im Erdkörper liefern. Rotationsbewegungen um die vertikale Achse in der P-Wellen Koda sind in diesem Zusammenhang von speziellem Interesse, da sie nur von horizontal polarisierten Scheerwellen angeregt werden können. Die gemessenen Rotationsbewegungen deuten folglich auf Konversionen von P- zu SH-Energie durch multiple Streuung an 3D-Heterogenitäten hin. Für die Bestimmung von Streu- und Dämpfungsparametern im Südosten Deutschlands (Gräfenberg Array, Vogtland) werden synthetisch erzeugte, mehrspurige Seismogrammeinhüllende mit Daten lokaler und regionaler Schwarmbeben und teleseismicher Ereignisse verglichen. In einem ersten Schritt werden frequenzabhängige Krustenparameter für die Vogtlandregion mittels eines lokalen Datensatzes von nahen Schwarmbeben bestimmt. Die Resultate mittels elastischer Energietransfertheorie werden mit Ergebnissen aus Simulationen mittels akustischer Energietransfertheorie verglichen. Beide Methoden liefern ähnliche Parameter und sagen einen größeren Einfluss der intrinsichen Dämpfung im Vergleich zur Streudämpfung voraus. Aus den elastischen Simulationen geht hervor, dass für die Beschreibung der Daten Vorwärtsstreung angenommen werden muss, die Stärke dieser lässt sich jedoch nicht auflösen. In einem zweiten Schritt werden die Streu- und Dämpfungseigenschaften der Erdkruste im Untergrund des Gräfenberg Arrays untersucht. Hierzu wird eine nicht-lineare genetische Inversion von Seismogrammeinhüllenden regionaler Ereignisse bei hohen Frequenzen (4–8 Hz) verwendet. Das bevorzugte Modell der Krustenheterogenität wird durch ein exponentielles Zufallsmedium, einer mittleren freien Transportweglänge von ca. 420 km und einem Qualitätsfaktor für S-Wellen Q iS von ca. 700 beschrieben. Ein letzter Schritt vergleicht Simulationen von teleseismischen P-Welleneinsätzen mit beobachteten Seismogrammeinüllenden von tiefen Erdbeben unter der Nutzung der Parameter aus der regionalen Inversion. Die Simulationen der teleseismischen Ereignisse mit den Parametern der regionalen Inversion zeigen eine gute Übereinstimmung mit den gemessenen Seismogrammeinhüllenden. Dieser Vergleich beinhaltet Ringlaserbeobachtungen der Rotationsbewegungen um die vertikale Achse, welche aus dem angenommenen Streumodell resultieren. Das Modell sagt voraus, dass die elastische Energie in der teleseismischen P-Wellen Koda im Gegensatz zur Koda lokaler oder regionaler Ereignisse nicht gleichverteilt ist, sondern einen Überschuss an Scheerenergie beinhaltet. Die Resultate aus den Untersuchungen der lokalen, regionalen und teleseismischen Datensätze zeigen, dass die Streuereignisse, welche die seismische Koda erklären, hauptsächlich in der Kruste unterhalb der seismischen Empfänger stattfinden. Streuung des Wellenfeldes im Mantel wird für die Erklärung der Daten nicht benötigt, schwache Streuung im lithosphärischen Mantel kann jedoch nicht ausgeschlossen werden.

Background: In PET/MRI, linear photon attenuation coefficients for attenuation correction (AC) cannot be directly derived, and cortical bone is, so far, usually not considered. This results in an underestimation of the average PET signal in PET/MRI. Recently introduced MR-AC methods predicting bone information from anatomic MRI or proton density weighted zero-time imaging may solve this problem in the future. However, there is an ongoing debate if the current error is acceptable for clinical use and/or research. Methods: We examined this feature for [18F] fluorodeoxyglucose (FDG) brain PET in 13 patients with clinical signs of dementia or movement disorders who subsequently underwent PET/CT and PET/MRI on the same day. Multiple MR-AC approaches including a CT-derived AC were applied. Results: The resulting PET data was compared to the CT-derived standard regarding the quantification error and its clinical impact. On a quantitative level, −11.9 to +2 % deviations from the CT-AC standard were found. These deviations, however, did not translate into a systematic diagnostic error. This, as overall patterns of hypometabolism (which are decisive for clinical diagnostics), remained largely unchanged. Conclusions: Despite a quantitative error by the omission of bone in MR-AC, clinical quality of brain [18F]FDG is not relevantly affected. Thus, brain [18F]FDG PET can already, even now with suboptimal MR-AC, be utilized for clinical routine purposes, even though the MR-AC warrants improvement.

Die Kombination von Positronen-Emissions-Tomographie (PET) und Röntgen-Computertomographie (CT) in Form moderner PET/CT-Geräte ermöglicht die Nutzung der CT-Information zur Korrektur der Photonenschwächung in der PET. Allerdings können Bewegungen, die zum Beispiel durch die Atmung hervorgerufen werden können, zu einer fehlerhaften Schwächungskorrektur führen. Die Einführung von zeitlich aufgelöster Bildgebung für beide Modalitäten (4D-PET/4D-CT) ermöglicht nicht nur die Auflösung von periodischen Bewegungen, sondern auch die Reduktion dieser Fehler in der Schwächungskorrektur. Dazu werden die einzelnen Datensätze des 4D-PET, die jeweils einer bestimmten Bewegungsphase entsprechen, mit dem entsprechenden CT-Datensatz dieser Atemphase schwächungskorrigiert. In der vorliegenden Arbeit wurde diese phasenkorrelierte Schwächungskorrektur des 4D-PET mit dem 4D-CT am Universitästsklinikum Dresden installierten PET/CT ermöglicht und anhand von Phantomexperimenten mit anderen Schwächungskorrekturmethoden für 4D-PET verglichen. Dazu musste zunächst die Aufnahme von 4D-CT an dem verwendeten PET/CT ermöglicht und dessen Synchronität mit dem 4D-PET hergestellt werden. Außerdem wurde ein vorhandenes Atemphantom so modifiziert, dass es typische Bewegungen von Bronchialkarzinomen in zwei Dimensionen und mit zwei möglichen Atemmustern simuliert. Die phasenkorrelierte Schwächungskorrektur führte zu einer quantitativ korrekten Wiederherstellung des Aktivitätsvolumens, der darin enthaltenen Aktivität sowie der Bewegungsamplitude und stellt somit die beste der hier verglichenen 4D-PET-Schwächungskorrekturmethoden dar. Diese Ergebnisse lassen vermuten, dass die phasenkorrelierte Schwächungskorrektur auch bei klinischer Anwendung eine signifikante Verbesserung in oben genannten Punkten darstellt. Dies sollte in Zukunft an Patientendaten überprüft werden. / The combination of Positron Emission Tomography (PET) and Computed Tomography (CT) in one device allows the use of CT-information for attenuation correction in PET. Though motion, for example induced by respiration, can cause inaccurate attenuation correction. The implementation of time-resolved imaging methods for both modalities (4D-PET/4D-CT) enables not only the resolution of motion but also the reduction of artifacts caused by attenuation correction. Therefore, the single datasets of the 4D-PET that are related to a individual respiratory phase, are attenuation corrected with the corresponding dataset of the 4D-CT. This phase correlated attenuation correction of the 4D-PET with the 4D-CT was implemented at the PET/CT installed at the Universitätsklinikum Dresden. For that purpose the acquisition of 4D-CT was implemented at the PET/CT and its synchronisation with the 4D-PET was verified. Furthermore the new attenuation correction method was compared with other attenuation correction methods by performing phantom experiments. Therefore an exisisting respiratory phantom had to be modified to perform typical lung tumor motion in two dimensions with two possible patterns of respiration. The phase correlated attenuation correction leads to a quantitatively correct restauration of the activity volume, its total activity and its motion amplitude. Compared with other correction methods, the phase correlated attenuation correction shows the best results in all examined criteria. This findings suggest that the clinical application of the phase correlated attenuation correction will also lead to a significant improvement in all mentioned points. This has to be verified by analyzing patient data.

Cette thèse s’inscrit dans le cadre de la préparation de la mission spatiale franco­-chinoise SVOM (Space Variable Object Monitor) opérationnelle à l’horizon 2021 et qui aura pour but de détecter et caractériser les sursauts gamma. La première partie de la thèse consiste au développement d’un logiciel de simulation de COLIBRI (Catching OpticaL and Infrared BRight transIents), un télescope de suivi au sol de SVOM. Il comporte un simulateur de sursauts gamma, un exposure time calculator, un simulateur d'image, un algorithme estimant le redshift photométrique. Il a permis d'une part d’estimer les performances scientifiques du télescope afin de s'assurer qu'elles satisfont le cahier des charges, et d'autre part d'estimer une précision sur l'estimation de la distance du sursaut de l'ordre de 10% et 15% sur le redshift pour les sursauts à 3.5 < z < 8 et z > 8. La deuxième partie de la thèse porte sur l’étude de la propriété des poussières au sein des galaxies dans lesquelles se produisent ces sursauts gamma, mais aussi à l’étude de leur activité de formation stellaire. J'ai comparé les courbes d'extinction mesurées sur la ligne de visée de 7 GRBs à la courbe d'atténuation de leurs galaxies à l'aide d'un code de SED fitting, CIGALE. En comparant à un code de transfert radiatif on obtient des informations sur la distribution étoiles / poussière au sein de ces galaxies. Sur un échantillon élargi, on montre que les courbes d'atténuation pentues (plates) corrèlent avec des galaxies avec une faible (grande) atténuation due à la poussière, les moins (plus) activent en formation stellaire et les moins (plus) massives / This thesis lies within the framework of the sino-french SVOM (Space Variable Object Monitor) mission to be launched in 2021 whose objective is the detection and characterisation of Gamma-Ray Bursts (GRBs). The first part consists in the development of an end-to-end software for COLIBRI (Catching OpticaL and Infrared BRight transIents), a SVOM ground follow-up telescope. It consists in a GRB simulator, an Exposure Time Calculator, an image simulator and a photometric redshift algorithm. It allowed to estimate the telescope scientific performances to ensure that the optical design was fulfilling the scientific requirements. The relative accuracy on the photometric redshift delivered by COLIBRI is estimated to be about 10% and 15% for GRBs at 3.5 < z < 8 et z > 8. The second part of the thesis deals mainly with the study of dust properties in GRB host galaxies, but also with the star formation activity in these galaxies. We compare extinction curves measured along the GRB line of sight for 7 GRBs to the attenuation curve of their host galaxies measured with CIGALE, a SED fitting code. By comparing these curves to the results of a radiative transfer code, we obtain information about the geometrical distribution of dust and stars in these galaxies. On a larger sample of 23 galaxies, we show that the steepest (flattest) attenuation curves are associated to galaxies with a large (small) amount of dust attenuation, less (more) active in star formation and less (more) massive galaxies

In a collaboration with limnologists at Uppsala University a planar HPGe detector has been used to find 210Pb in lake bottom sediment in hopes of getting an estimation for the sedimentation rate. Using a least squares fit to the data, the sedimentation rate was calculated to 0.08 ± 0.01 cm/year with background subtraction, and with it a timescale for the age of the sediment could be implemented. With this timescale the lake bottom sediment can be dated as far back as a century. Along with this, using 137Cs traces from the Chernobyl disaster of 1986 a consistency check can be made for our timescale. It is estimated that the Chernobyl disaster occurred at 1989 ± 7 years which is consistent with reality since 1986 is included in the interval 1982-1989. This helps us validate the determined sedimentation rates.

Impacts from mining waste deposits on groundwater resources have been recognized invarious parts of the world; though varied in scale depending on the composition of mineralsbeing mined, the level of technology employed and environmental commitment of thedevelopers. Mining activities usually involve milling, concentrating, and processing of oreswhich will result in a huge amount of waste, called tailings, usually deposited inimpoundments as a slurry, composed of fine grained geological material (uneconomicalminerals), chemicals utilized in the processs, and water. Oxidation of these deposits, usuallycontaining sulphide minerals, may result in generation of an acidic, metal laden leachate,callled Acid Mine Drainage (AMD), which may have a devastating impact on thesurrounding groundwater resources. In this study, the stochastic LaSAR-PHREEQC reactive transport modeling approach is usedin order to evaluate the coupled effect of geochemical reactions and physical heterogeneity ofthe subsurface in the breakthrough of acidity and metal downstream of the source while theAMD transported in the water saturated zone of an impoundment. The tailings depositcalled Impoundment 1 at the Kristineberg mining site at the Skellefteå field, in northernSweden, is used as a case study to simulate pH buffering processes and attenuation of Zn.The objectives of the study are 1) to evaluate the relevance of different possible geochemicalprocesses in pH buffering and Zn attenuation; 2) to evaluate the effect of spatial variability ofthe physical processes of the groundwater system on the breakthrough of contaminants; and3) to evaluate the effect of the location and distribution of the source zone in terms of thedistance from the impoundment boundary. Simulation results of the presented model revealed that pH buffering from calcite andchlorite are important processes capable of counteracting the acidification from AMD.Dissolution of secondary Al(OH)3(s) is another important process capable of buffering pH.Precipitation of smithsonite, ZnCO3, is an important process for attenuation of Zn2+.Moreover, sorption of Zn2+ on ferric iron surfaces is found to be an important process forattenuation of the metal, depending on the available sorption surface sites. Flow variabilityhighly affects the breakthrough of the contaminants such that with increasing subsurfaceheterogeneity, earlier breakthrough of contaminants occurs. Moreover, increased variabilityresults in decreased peak loads, but longer duration of the load. / www.ima.kth.se