Le processus de recombinaison V(D)J des gènes d’immunoglobulines (Ig) est caractérisé par une grande imprécision des jonctions entre les segments variables (V), de diversité (D) et de jonction (J). Deux fois sur trois, un décalage du cadre de lecture apparaît, aboutissant à une jonction non productive dite « hors phase ». Plusieurs études ont démontré que les deux allèles productifs et non-productifs sont activement transcrits. Les transcrits matures issus des allèles non-productifs sont pris en charge par un mécanisme de surveillance des ARNm appelé NMD « Nonsense-Mediated mRNA Decay ». En dégradant efficacement les ARNm d’Ig contenant des codons non-sens, ce mécanisme prévient l’apparition des Ig tronquées au cours de l’ontogénie B. Néanmoins, aucune étude n’a jusqu’ici analysé l’impact de l’épissage alternatif des transcrits d’Ig non-productifs. Ce phénomène appelé NAS « Nonsense-associated Altered Splicing » peut conduire à une production d’Ig tronquées présentant des délétions internes du domaine variable (V).Les projets développés lors de cette thèse ont montré que la présence d’un codon non-sens, au niveau de l’exon variable (VJ) des transcrits Igκ, favorise le saut d’exon et la production de chaînes légères dépourvues de domaine variable (ΔV-κLCs). De façon intéressante, ces Ig tronquées provoquent un stress cellulaire et conduisent à l’apoptose des plasmocytes (Article 1). Ces observations ont permis d’identifier un nouveau point de contrôle agissant tardivement lors de la différenciation plasmocytaire : le TIE « Truncated-Ig Exclusion » checkpoint. Ce processus de contrôle provoque l’élimination des plasmocytes qui produisent des chaînes d’Ig tronquées. Nous avons également étudié l’épissage alternatif des transcrits d’Ig non-productifs en l’absence de TIE-checkpoint (Article 2). Cette étude a révélé que l’hypertranscription des gènes d’Ig dans les plasmocytes favorise l’épissage alternatif des transcrits d’Ig non-productifs. En utilisant un modèle d’expression forcée d’Ig tronquées, nous avons mis en évidence une coopération entre les mécanismes assurant la surveillance des ARNm (NMD) et la surveillance au niveau protéique (UPR : « Unfolded Protein Response », autophagie) (Article 3). Sur la base de ces résultats, nous avons mis au point une nouvelle approche thérapeutique qui consiste à forcer la production d’Ig tronquées en utilisant des oligonucléotides anti-sens (AON) capables de provoquer l’élimination de l’exon variable lors de l’épissage. Cette invention pourrait ouvrir des perspectives thérapeutiques pertinentes dans le traitement du Myélome Multiple et d’autres pathologies touchant les plasmocytes. / The recombination process V(D)J of immunoglobulin (Ig) genes is characterized by random junctions between the variable (V), diversity (D) and joining (J) segments. A frameshift mutation appears in two-third of cases, generating a non-productive or « out of frame » junction. Several studies have shown that both productive and non-productive alleles are actively transcribed. The mature transcripts from nonproductive alleles are usually considered sterile and innocuous as a result of an mRNA surveillance mechanism called NMD « Nonsense-Mediated mRNA Decay ». By degrading aberrant mRNA, this mechanism prevents the appearance of truncated Ig during B cell ontogeny. However, less is known about the impact of alternative splicing on non-productive Ig transcripts. This mechanism, called NAS « Nonsense-associated Altered Splicing » can lead to the production of truncated Ig with internal deletions of variable domain (V). During my thesis, we have shown that the presence of a stop codon, within the variable exon (VJ) of Igκ transcripts, promotes exon skipping and synthesis of V domain-less κ light chains (ΔV-κLCs). Interestingly, such truncated Ig causes cellular stress and leads to plasma cells apoptosis (Article 1). These findings have identified a new checkpoint acting late during plasma cell differentiation: TIE « Truncated-Ig Exclusion » checkpoint. This process ensures counter-selection of plasma cells producing truncated-Ig. We also studied the alternative splicing of non-productive Ig transcripts in the absence of TIE-checkpoint (Article 2). We found that hypertranscription of Ig genes in plasma cells promote alternative splicing of non-productive Ig transcripts. Using a model forcing the expression of truncated Ig, we identified a cooperative action between mRNA surveillance mechanisms (NMD) and those of protein surveillance (UPR « Unfolded Protein Response », autophagy) (Article 3). Based on these results, we have developed a new therapeutic approach by increasing the production of truncated Ig using antisense oligonucleotides (AON) that leads to the elimination of the variable exon during splicing. This invention could open new avenues for the treatment of Multiple Myeloma patients and other pathologies affecting plasma cells.

Spirituality is a word in frequent use in contemporary society. In a broad sense it refers to the 'raison d'etre' of our existence, the meaning and values to which we ascribe. Everyone embodies a spirituality in this wider sense, whether it be nihilistic, materialistic, humanistic or religious. The present study evaluates the phenomenon of the Toronto Blessing in the light of spirituality in general and Christian spirituality in particular. By means of a broadly-based phenomenological methodology, the manifestations accompanying the Toronto Blessing are evaluated firstly, with respect to the Bible; secondly, with respect to the Hindu experience of 'Kundalini awakening'; and thirdly, in terms of neuroscience and certain psychological processes, such as hypnosis, mass hysteria, and the role of body and mind in creating spiritual experiences. Although Charismatics claim that the Toronto Blessing has a sound biblical foundation, no evidence to support this claim has been found. However, striking similarities are found between the manifestations of the Toronto Blessing and the techniques used in the 'Kundalini awakening' for the transference of energy. Finally, the major findings of this study support the conclusion that the Toronto Blessing is largely the result of psychological techniques. The possibility of Godly intervention is not totally excluded, but caution is urged, so as to be aware of extraneous factors that create similar manifestations. While it is agreed that the Toronto Blessing can be seen as an expression of spirituality in a broad sense, nevertheless it cannot be viewed as an expression of Christian spirituality in the Charismatic Movement. / Christian Spirituality, Church History and Missiology / D. Th. (Christian Spirituality)

Thesis (MEdPsych (Educational Psychology))--Stellenbosch University, 2008. / Life orientation is fundamental in empowering learners to live meaningful lives. Life-skills education and training forms part of life orientation and helps to address the abovementioned aspect. Life-skills education and training falls under the most recent and powerful approaches in handling psychological problems. It strives to adapt the principles of psychology and social science into teachable skills to ensure intra-personal effectiveness and improve the general quality of peoples' lives. The aim of this pilot study was to alter The Path programme to design and develop an intrapersonal life-skills programme aimed at enhancing the self-concept of youth offenders. The intervention research model of Rothman and Thomas (1994) was employed as foundation for the design and development of The Altered Path programme. The intervention research model was chosen as it aimed at creating a means to address problems experienced by individuals and society. It strives to improve the life, health and well-being of individuals and society. This purposeful sampling consisted of six Afrikaans-speaking, male youth offenders from the Drakenstein Juvenile Centre. The Altered Path programme was implemented in three stages. The first stage consisted of sessions one to seven. The following topics were covered during this stage: introduction and programme orientation, conceptualisation of mission statements and elements, past personality influences and Cup of Sorrow, the Positive Prophecy and Talent Shield, the mission statement, the vision statement and goal setting. The second stage, which consisted of sessions eight and nine, entailed the training of the participants for service learning. The third stage entailed the implementation of the service learning process. The study found that The Altered Path programme could contribute towards a growth in intra-personal skills with regard to self-awareness, personal goal setting, spirituality, morals and values, self-esteem and self-regulation (both emotional and cognitive). The result of the intra-personal growth was that the interpersonal skills with regard to communication and helping skills of participants improved. In answer to the research question the finding was made that the intra-personal development positively affected the following dimensions of their self-concept: personal, ideal, cognitive, spiritual, social self and psychological self. In the light hereof the statement can be made that The Altered Path programme seems to have a positive effect on the overall self-concept of the participants. Conscious development of self-knowledge was identified as an element of the programme that could be refined to ensure that all participants develop self-knowledge in the same way during the field study.

Au croisement de l’anthropologie médicale, de la communication, du design industriel et des nouvelles technologies de l’information, ce mémoire se penche sur les difficultés communicatives rencontrées par le personnel médical et les patients de provenances culturelles variées dans le milieu des urgences hospitalières à Montréal. Dans l’optique d’améliorer l’échange au poste de triage, la réflexion porte principalement sur la pertinence et les caractéristiques d’un support visuel d’appoint. Elle aborde la problématique par l’étude des concepts de perception, de compréhension, d’interprétation et de représentation graphique sous les angles théoriques de la communication visuelle (Benjamin, Berger, Dibi-Huberman), de la sémiologie du discours social (Verón) et du jeu herméneutique (Gadamer). Les systèmes symboliques de cent cinquante images, illustrant sur Internet trois symptômes propres à la gastro-entérite, ont été analysés d’après une méthodologie mixte quantitative et qualitative afin d’identifier leurs sens dénotatifs et connotatifs. Les résultats appuient le recours à des images pour contourner les barrières langagières et révèlent l’existence d’une culture médicale visuelle internationale dont le code iconographique est hybride et pluriculturel. Ces nouvelles informations indiquent des critères de performance et des hypothèses concernant les changements occasionnés par l’ajout d’un support visuel dans la dynamique communicationnelle de l’ETC. La recherche ouvre aussi une piste vers l’étude herméneutique du produit en design industriel. / At the crossroads of anthropology, communicational and technology approach, this dissertation aims to provide a vision of the emergency room where cultural differences between the medical staff and the patients confront themselves creating communicational difficulties. To overcome the linguistic barriers, a visual support is considered as an alternative solution. In this way, the problematic is seen through the concepts of perception, comprehension, interpretation and graphic representation witch are examined by the visual culture, semiotic and hermeneutic theories. The symbolic systems of 150 images illustrating three gastro-enteritis symptoms have been analyzed following a quantitative and qualitative methodology in order to reveal their denotative and connotative significations. The results showed the existence of a new visual culture that presents a hybrid and pluricultural iconographic code. Based on these findings, new hypothesis concerning the impact of a visual support on the communicational dynamic could be proposed.

In dieser Arbeit wurde die Induktion spezifischer, immunologischer T-Zelltoleranz als therapeutische Strategie bei Autoimmunerkrankungen im Mausmodell untersucht. Da davon ausgegangen werden muss, dass viele der Autoimmunkrankheiten durch T-Zellen vermittelt sind, ist die Induktion spezifischer T-Zelltoleranz eine besonders interessante Therapiestrategie. Spezifische T-Zelltoleranz kann mittels Injektion des entsprechenden Peptidantigens induziert werden. Insgesamt sind zur Induktion einer solchen Toleranz, zumindest beim Menschen, relativ hohe Dosen an Peptidantigen notwendig. Die Produktion dieser Peptidantigene ist teuer. Bei unvorsichtiger Gabe kann es zur Anaphylaxie kommen. Es sollte also von Vorteil sein, die zu applizierende Menge an Peptid möglichst gering, dabei aber effizient zu halten. Vermutlich werden Antigene in Form von Peptiden schnell von unspezifischen Peptidasen und Proteasen in nicht-immunogene Fragmente gespalten und ausgeschieden, was wiederum eine hohe Dosierung erforderlich macht. Im Anfang der vorliegenden Arbeit stand die Hypothese, dass eine Stabilisierung des zu applizierenden Antigens zum Schutz vor Fragmentierung (und damit Wirkungsverlust) eine geeignete Methode sein könnte, Toleranzinduktion effektiver bzw. kostengünstiger zu gestalten. Bezüglich einer Stabilisierung von Peptiden zeigte sich, dass Peptide, welche aus rechtsdrehenden (D-)Aminosäuren zusammengesetzt sind, nur verzögert durch Proteasen/Peptidasen abgebaut werden. Wir setzten deshalb in dieser Arbeit D-Aninosäuren-substituierte Peptid-Varianten des Ovalbumin323-339-Peptidepitops (OVA323-339) ein und betrachteten die Wirkung dieser Peptide in vitro sowie in vivo auf spezifische DO11.10 T-Zellen. Basierend auf dem Peptidantigen OVA323-339, wurde zunächst ein minimales Epitop definiert, welches bei etwa gleicher Potenz um 6 Aminosäuren verkürzt werden konnte. Anschließend wurde eine Substitutionsanalyse durchgeführt, in der die ursprüngliche Aminosäuresequenz durch Austausch einiger L-Aminosäuren mittels D-Aminosäuren verändert wurde. Diese neu synthetisierten Peptide wurden zunächst auf ihre Fähigkeit überprüft, die OVA323-339 spezifischen DO11.10 T-Zellen in vitro zu aktivieren. Parallel konnte gezeigt werden, dass diese synthetisierten Peptidepitope in vitro eine deutlich verlängerte Serumhalbwertszeit aufwiesen. Im Weiteren wurde versucht, durch systemische Injektion von 300µg D-Peptid-Varianten in BABLB/c Mäusen T-Zelltoleranz zu induzieren. Die ex vivo restimulierten Lymphknoten-Zellen dieser Mäuse präsentierten je nach appliziertem Peptid eine reduzierte Proliferationsbereitschaft und IL-2 Sekretion. Die hier induzierte Toleranz konnte bis zu 60 Tagen post injectionem sowohl für das OVA323-339 als auch für einige der eingesetzten D-Peptide nachgewiesen werden. Auch nach Reduktion der Peptiddosis auf nur 100µg/Maus, waren die verkürzten und D-Aminosäuren-substituierten Peptide immer noch in der Lage sicher Toleranz zu induzieren. Die induzierte Toleranz durch D-Peptide war dabei der durch das Ausgangspeptid OVA323-339 induzierten Toleranz vergleichbar stark. Mit der Hilfe eines Transfermodells in unmanipulierte Mäuse, wurde das Verhalten der spezifischen T-Zellpopulation in vivo beobachtet. Durch den Transfer konnten in den Empfängermäusen (Balb/c) definierte T-Zellpopulationen bekannter Größe erzeugt werden. Mit dem Antikörper KJ1-26.1, der spezifisch den DO11.10-T-Zellrezeptor erkennt, konnten die transferierten Zellen in Geweben der Empfängermaus per FACS-Analyse nachgewiesen und deren Verhalten ex vivo studiert werden. Die intravenöse Injektion der serumstabilisierten Peptidanaloge führte in den transferierten Mäusen je nach Peptid zu einer funktionellen Nichtreaktivität (Anergie) als auch zur Deletion der für das Ausgangs(L-)Peptid spezifischen DO11.10 T-Zellen. In den oben genannten Versuchen ergaben sich Hinweise dafür, dass die D-Peptide ebenso effektiv sind wie das wesentlich längere Ausgangspeptid OVA323-339. Zukünftige Experimente werden weitere Aufschlüsse über einen möglichen Vorteil des Einsatzes von D-Peptiden in der Toleranzinduktion erbringen. / Induction of antigen-specific peripheral T cell tolerance in autoimmune diseases is an interesting therapeutically strategy. It can be induced by systemic injection of high-dose antigen. Investigations in induction of peripheral T cell tolerance in autoimmune mouse models revealed promising results. But it was also shown that the induced T cell tolerance spontaneously reverses after a period of time. This is probably due to a short in vivo half-life of the administrated peptide antigens. Since durable tolerance is required for this strategy to be of therapeutic value the administrated antigen-dose has to be of a very high and has to be injected repeatedly, and therefore bears an increased risk of anaphylactic reactions or exacerbation of the autoimmune disease. Because of these restrictions and also the high costs of peptide-production and purification, it is not surprising that this therapy didn t really find its way in to the clinical practice. The discovery that Peptides assembled partly or totally from D-amino acids are much more stable to proteolysis then natural L-peptides and therefore show an increased stability, lead to a wide interest of pharmacologists and immunologists. In former investigations it was shown that D-peptides used as vaccines elicited high levels of neutralizing antibodies so that there is no doubt about their immunogenic potency in vivo. It is also known that a single T cell receptor recognizes a wide range of peptide analogues that closely mimic the natural antigen. These observations led to our hypothesis, that the induction of peripheral T cell tolerance by systemic administration of D-Peptide substituted antigen variants should be possible and could be much more effective than the induction by the wild-type L-peptide. To verify our hypothesis we have chosen the well known OVA323-339 antigen which is recognized by T cells through the presentation in the I-Ad context. In a first step we performed a truncation analysis of OVA323-339 to identify a minimal epitope in it. We were able to demonstrate that the sequence OVA327-337 is as well potent as the original and 6 amino acids longer OVA323-339 sequence. The potency of new defined epitopes was tested by stimulating the OVA323-339 -specific DO11.10 T cells in vitro. In a stepwise performed substitution analysis we attempted to insert some D-amino acids in this novel peptide epitope. The DO11.10 cells only tolerated a few D-amino acid substitutions into the original sequence with the effect of now showing reduced proliferation. Performing an analysis of their half-life in vitro we identified two peptides as interesting candidates for the in vivo tolerance induction experiments. In the in vivo part of this work we induced peripheral tolerance by injecting the novel peptides into BALB/c mice. To monitor the behaviour of the tolerated T cells we also performed adoptive transfer experiments by transferring DO11.10 cells into naive BALB/c mice. With the help of the KJ26-1 antibody which specifically recognizes the DO11.10 T cell receptor it became possible to detect the transferred T cells ex vivo. Our results demonstrate that induction of peripheral T cell tolerance through injection of D-peptides is possible and long lasting (up to 60 days). Even with a dose reduction we found a stable T cell tolerance under ex vivo restimulation with the original peptide. Summarizing we were able to show that D-peptides are at least as effective as the natural occurring L-peptides inducing tolerance. Much more, the transfer experiments revealed that the kind of induced T cell tolerance (i.e. anergy and/or deletion through activation induced cell death) is antigen dependent and probably differs due to the agonistic potency of the given antigen.

Drugs, Danger, Delusions (and Deleuzians?) opens up a philosophical investigation into a series of ‘extreme’ mind and body films drawn from different historical contexts. Through two sections and four distinct chapters, cinema is explored as an agent of becoming that allows viewers to think and feel in an affected manner. Investigating a broad spectrum of extreme narratives focusing on drugs, hooligan violence, insomnia and madness, the project provides a focused historical understanding of the films’ affective regimes and aesthetic agendas. The different lines of flight and escape explored on-screen all somehow appear to spiral around the same issues, concepts, ideas and philosophies. Utilising the cinematic theories of Gilles Deleuze along with his philosophical work co-authored with Félix Guattari, the thesis aims to investigate a range of related films, that in the extreme, reveal underlying models of an integrated or parallel mind and body and immanently embedded identity; wherein the concept of a stable and fixed being is replaced by that of a fluid becoming. All chapters investigate how immanently embedded characters embark upon extreme or dangerous lines of escape, where the reinvention of living and thinking is explored and made visible. The first section investigates a range of ‘head-films’ that take the mind as their theme, but are found to plicate and expand consciousness into the parallel body. The second section investigates extreme body films that push the sensory-motor schema to its limits so that thought, perception and consciousness become affected. The two interrelated sections investigate how the films and filmmakers employ different regimes of mind and body cinema to aesthetically convey and relay these concepts to the spectator. The project thus strives to develop Deleuzian paradigms beyond their original scope to explore parallel-image regimes and sequences that allow spectators to think and feel the films’ underlying philosophical concepts and positions.

Made available in DSpace on 2016-04-26T18:17:15Z (GMT). No. of bitstreams: 1 Gustavo Garcia da Palma.pdf: 1137319 bytes, checksum: 76b5b92accf97fed4960813084961ce6 (MD5) Previous issue date: 2008-04-28 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / It s very common Nowadays the use of technologies in communication processes in art. The coupling of prosthesis to the body and the cross-breeding of scenic languages happen. The analysis of these processes, most of them at least, is done under the director s, the choreographer s or the critic s perspective. The objective is the investigation of consciousness alteration for the actor based on the trance technique proposed by Jerzy Grotowski (1979, 2007), for a theater poor in technology . The hypothesis is the application of this concept in an art rich in technology: the Artmedia (MACHADO, 2002). An undisciplined and theoretical-practical methodology was used. M. McLuhan (1969, 1972), S. Pinker (1998, 2002), Y. R. G. Araújo (2005) e de P. M. Aguiar (2006) made it possible to conceive the technology as an organic/environmental process and overcome the dualism rich-poor inherent in Grotowski. The concept syntheticbody , created from the concept corpomídia (KATZ & GREINER, 2005), represents the refusal in altering itself. T. Richards (2001), R. Cohen (2004, 2003), M. Bonfitto (2002), R. Ferracini (2003), E. Barba (2006), R. Demarcy (in GUINSBURG, 2003) and others allowed a new interpretation of important theater authors like Aristóteles (1966), C. Stanislávski (1979) e J. Grotowski (op. cit.). A. Damásio (2000, 2004), S. Pinker (1998), D. C. Dennett (1997, 2006), Lakoff & Johnson (2002), R. Dawkins (2001a, 2001b), and others allowed the conception of consciousness in a healthy state, here, a parameter to identify and create altered states, which can be systemically understood from J. Vieira (2006, 2007). Consequently the actor s training system was called Alteration Procedures , and Jerry Grotowski s interpretation of the trance technique for the body could be reviewed and enlarged in high levels of technology in communication processes / É cada vez mais comum a utilização de tecnologias nos processos comunicacionais em arte. Acoplamentos de próteses ao corpo e hibridização de linguagens cênicas se sucedem. Tais processos, em sua maioria, são realizados sob a perspectiva do diretor, do coreógrafo ou do crítico de arte. Aqui é proposta uma análise pelo corpo em ação no meio. O objetivo é a investigação da alteração de consciência para o ator, com base na técnica de transe , proposta por Jerzy Grotowski (1979, 2007), para um teatro pobre em tecnologia. A hipótese é a aplicação deste conceito em uma arte rica em tecnologia: a Artemída (MACHADO, 2002). Utilizou-se de metodologia indisciplinar e teórico-prática. Autores como M. McLuhan (1969, 1972), S. Pinker (1998, 2002), Y. R. G. Araújo (2005) e P. M. Aguiar (2006) possibilitaram conceber a tecnologia como processo organismo/meio, e superar a dualidade rico-pobre inerente em Grotowski. O conceito corposintético , criado a partir do conceito corpomídia (KATZ & GREINER, 2005), representa a recusa em alterar-se. T. Richards (2001), R. Cohen (2004, 2003), M. Bonfitto (2002), R. Ferracini (2003), E. Barba (2006), R. Demarcy (in GUINSBURG, 2003) e outros permitiram a releitura de importantes autores da área teatral como Aristóteles (1966), C. Stanislávski (1979) e J. Grotowski (op. cit.). A. Damásio (2000, 2004), S. Pinker (1998), D. C. Dennett (1997, 2006), Lakoff & Johnson (2002), R. Dawkins (2001a, 2001b), e outros permitiram conceber os conceitos de consciência para ser possível identificar e criar estados alterados, entendidos sistemicamente a partir de J. Vieira (2006, 2007). Os princípios de treinamento do ator são tratados como Procedimentos de Alteração, e a leitura da técnica de transe de Jerzy Grotowski pôde ser revista e ampliada para o corpo em ambientes com altos índices de tecnologia nos processos comunicacionais

Avec l’avancée en âge, l’efficience de l’ensemble des fonctions cognitives, en particulier la mémoire épisodique, tend à diminuer. L’étude des effets Dm et old/new et des résultats de la méthode sLORETA a permis de caractériser sur un plan neurofonctionnel deux types de vieillissement non pathologique chez les seniors. D’une part, le vieillissement cognitif stable se traduit par des processus d’encodage identiques à ceux observés chez des sujets jeunes alors que les processus de récupération nécessitent un recrutement bilatéral des lobes temporaux médians, cette activation étant unilatérale chez les sujets jeunes. Ces résultats vont dans le sens du modèle HAROLD qui suggère une réduction de l’asymétrie hémisphérique avec l’âge. D’autre part, le vieillissement cognitif altéré proviendrait d’une altération des réseaux cérébraux dès la phase d’encodage. La baisse d'activité au niveau du cortex préfrontal gauche, siège des fonctions exécutives, suggèrerait une difficulté de mise en œuvre de stratégies d'encodage efficaces et aurait un impact direct sur l'activité observée au niveau du lobe temporal médian de façon bilatérale ce qui pourrait donc expliquer une difficulté de stockage de l'information. / With advancing age, the efficiency of all cognitive functions, especially episodic memory, tends to decrease. The study of Dm and old/new effects and of the results of the sLORETA method allowed to characterize, on a neurofunctionnal level, two types of non-pathological aging among seniors. On the one hand, stable cognitive aging results in encoding processes similar to those observed in young subjects while the retrieval processes require a bilateral recruitment of the medial temporal lobes, this activation being unilateral in young subjects. These results are in line with the HAROLD model, suggesting a reduction of hemispheric asymmetry with age. On the other hand, the altered cognitive aging might come from an alteration of brain networks at the early stage of the encoding phase. The decline of activity in the left prefrontal cortex, seat of executive functions, would suggest a difficult implementation of effective encoding strategies and have a direct impact on the activity observed in the medial temporal lobe bilaterally which could therefore explain information storage difficulties.

Grave X was found in 1952 during construction work in Tuna in Badelunda parish, in the province of Västmanland. Objects from this 3rd Century grave were dispersed and the stone grave covering and cist-like wooden burial chamber were cut almost in half as a result of the construction work that unearthed it. The purpose of this dissertation is to create a better understanding of Tuna in Badelunda and to place Grave X and the person buried there in context. Due to my interest in Grave X and the person in this grave, the scope of the study is limited to Tuna during the Late Roman Iron Age. What kind of place may Tuna in Badelunda have been during that time? Which kinds of knowledge may the person in Grave X have possessed and what roles may this person have had in local society? How may this person have acted in Tuna in Badelunda in particular? Why was this person buried in the specific type of structure that was Grave X? To answer these questions, ancient monuments and phenomena in the Tuna area, objects from the grave and construction details of the grave are discussed. Specifically, I examine the name Tuna, stone enclosures, hillforts of Bejby borg-character and travel routes, beads, golden rings in the shape of snakes, vessels and serving utensils, and the stone grave covering and cist-like chamber. Since Grave X was partly ruined when discovered, comparisons are made to about 20 similar graves from other parts of Scandinavia in order to get an idea of what may have been lost from Grave X. A performative-constructive gender perspective is of importance in this dissertation, as well as the concept of creolization. The kinds of knowledge and the societal roles the person in Grave X may have had can be summarized in five categories or contexts of action: production within the (social-political) economy of the farm, ritual performances, physical communication, textile production, and oral performances with the telling of stories and relating of memories. Possible personal strategies in relation to the activities the person in question was involved in are seen as important. One way this dissertation takes up this subject is through the discussion of the role the person may have had in greetings and farewells in the yard of the farm (Sw. tun, gårdsplan). Greetings and farewells were probably of importance, and Tuna is discussed as a crossroads. This means that although a local perspective is advocated in this dissertation, Tuna may not be seen as an isolated community, but rather as a small place that to a great extent partook in the larger world. This can also be seen in Grave X; when the person in this grave was buried, the living made choices that both expressed local traditions and made reference to far-away places. In contrast to the surrounding graves, the person in Grave X was not cremated. One of many possible reasons may have been a desire to emphasize the person’s personality and gender as well as roles in society. / <p>Auktoriserad namnform i LIBRIS: Fernstål, Charlotte, 1974-</p>

Au croisement de l’anthropologie médicale, de la communication, du design industriel et des nouvelles technologies de l’information, ce mémoire se penche sur les difficultés communicatives rencontrées par le personnel médical et les patients de provenances culturelles variées dans le milieu des urgences hospitalières à Montréal. Dans l’optique d’améliorer l’échange au poste de triage, la réflexion porte principalement sur la pertinence et les caractéristiques d’un support visuel d’appoint. Elle aborde la problématique par l’étude des concepts de perception, de compréhension, d’interprétation et de représentation graphique sous les angles théoriques de la communication visuelle (Benjamin, Berger, Dibi-Huberman), de la sémiologie du discours social (Verón) et du jeu herméneutique (Gadamer). Les systèmes symboliques de cent cinquante images, illustrant sur Internet trois symptômes propres à la gastro-entérite, ont été analysés d’après une méthodologie mixte quantitative et qualitative afin d’identifier leurs sens dénotatifs et connotatifs. Les résultats appuient le recours à des images pour contourner les barrières langagières et révèlent l’existence d’une culture médicale visuelle internationale dont le code iconographique est hybride et pluriculturel. Ces nouvelles informations indiquent des critères de performance et des hypothèses concernant les changements occasionnés par l’ajout d’un support visuel dans la dynamique communicationnelle de l’ETC. La recherche ouvre aussi une piste vers l’étude herméneutique du produit en design industriel. / At the crossroads of anthropology, communicational and technology approach, this dissertation aims to provide a vision of the emergency room where cultural differences between the medical staff and the patients confront themselves creating communicational difficulties. To overcome the linguistic barriers, a visual support is considered as an alternative solution. In this way, the problematic is seen through the concepts of perception, comprehension, interpretation and graphic representation witch are examined by the visual culture, semiotic and hermeneutic theories. The symbolic systems of 150 images illustrating three gastro-enteritis symptoms have been analyzed following a quantitative and qualitative methodology in order to reveal their denotative and connotative significations. The results showed the existence of a new visual culture that presents a hybrid and pluricultural iconographic code. Based on these findings, new hypothesis concerning the impact of a visual support on the communicational dynamic could be proposed.