A discussion of the artistic education and development of the author, Artemis Skevakis Jegart, along with a commentary on the paintings of his one man exhibition. / "January, 1953." / Typescript. / "Submitted to the Graduate Council of Florida State University in partial fulfillment of the requirements for the degree of Master of Arts." / Advisor: Edmund D. Lewandowski, Professor Directing Paper. / Includes bibliographical references (leaf 40).

Die Arbeit untersucht den Bezug der Artemis zu Frauen. Neben den meist in der Forschungsliteratur erörterten Aspekten der Artemis als Geburtsgöttin und Beschützerin der Kindheit und der Jugend leistet Artemis bei entscheidenden biologischen Ereignissen im Leben von Frauen Beistand, die den gynäkologischen Aspekt vor Augen stellen. Es handelt sich dabei um die Menarche, die eheliche Vereinigung, die Entbindung und das Wochenbett. Zu diesem Zweck wurden im ersten Teil der Arbeit einschlägige Funktionsepiklesen zusammengestellt, die Zeugnis von diesem Wirkungskreis der Artemis ablegen. Mit Bezug auf die aus dem ersten Teil gewonnenen Ergebnisse untersucht der zweite Teil der Arbeit diese Hilfe der Artemis anhand von Kulten auf der Südwestpeloponnes. / This work examines the relationship of Artemis to Women. Along with the most discussed aspects of Artemis in the research bibliography as a goddess of childbirth and protectress of childhood and youth Artemis provide assistance in important biological events of women’s lives, i.e. menarche, marital intercourse, delivery and puerperium, which reveal the gynaecological aspect of the goddess. For this purpose, in the first part of this work functional epicleses are collected, which provide evidence for these spheres of influence of Artemis. With regard to the results of the first part of the work, the second part examines Artemis’ assistance on the basis of cults in Southwest Peloponnes.

La fonction des protéines est intimement liée à leur structure et à leur dynamique. La Résonance Magnétique Nucléaire est une technique de choix permettant d'étudier ces deux aspects à une résolution atomique. La relaxation du spin des noyaux d'azote-15 permet de quantifier ces mouvements aux échelles de temps pico- nanosecondes grâce à la determination de la fonction de densité spectrale, décrivant les mouvements du vecteur NH amide. Il est essentiel de mesurer les vitesses de relaxation à des champs magnétiques faibles pour mieux décrire les mouvements nanoseconde. De telles mesures sont possibles grâce à la relaxométrie haute-résolution et ont été réalisées sur l'ubiquitine. Celles-ci ont permis la caractérisation de mouvements nanoseconde dans les parties flexibles de l'ubiquitine. L'interprétation des données de relaxation pour des protéines désordonnées requiert le développement de modèles spécifiques à ces protéines. Nous avons développé une approche, appelée IMPACT, permettant une reconstruction mathématique de la fonction de densité spectrale. Appliquée au facteur de transcription Engrailed 2, cette approche a permis d'accéder à la distribution d'échelles de temps ps-ns à partir de données de relaxation à haut champ. Cette approche, combinée à des mesures de relaxométrie sur la région C-terminale de la protéine Artemis, devrait permettre d'obtenir une représentation fidèle et précise de la dynamique d'une protéine désordonnée. De plus, nous avons étudié la cinétique et la thermodynamique de l'interaction entre Artemis et la Ligase IV. Nos travaux ont permis de développer de nouvelles approches pour l'analyse de larges ensembles de données de relaxation. / The intimate relation between the structure, dynamics and function of biomolecules is widely recognized. NMR is a unique technique to extract information on both structure and dynamics at atomic resolutions. Measurements of nitrogen-15 nuclear spin relaxation allow a quantitative description of motions on pico-nanosecond timescales through the characterization of the spectral density function (SDF), which describes the motions of amide bonds in proteins. The SDF has to be sampled at low magnetic fields, inappropriate for protein NMR, in order to obtain a better description of motions. Such measurements are possible by the use of high-resolution relaxometry. Such measurements on Ubiquitin highlight the sub- and low-nanosecond motions in flexible regions. The classical models for the interpretation of relaxation data in proteins are not well suited for intrinsically disordered proteins (IDPs) and require the development of new approaches. We developed a new approach, called IMPACT, based on a mathematical reconstruction of the distribution of correlation times from the experimental SDF. We have applied IMPACT to the transcription factor Engrailed 2. Our method allowed an unprecedented description of the distribution of pico- to nanosecond motions in IDPs. The IMPACT approach will be combined with high-resolution relaxometry measurements on the C-terminal region of the protein Artemis to provide information on an IDP. In addition, we have described the kinetics and thermodynamics of the interaction of Artemis with the DNA Binding Domain of Ligase IV.Overall, this work contributes to the development of new concepts for the interpretation of extensive nuclear spin relaxation data in proteins.

La voie de réparation NHEJ (Non-Homologous End-Joining) est une voie majeure de réparation des cassures double-brin chez l’homme. Les protéines de cette voie interagissent et forment des complexes dynamiques dont les mécanismes moléculaires sont encore largement méconnus. Nous avons dans un premier temps mis au point des protocoles de production à l’échelle de plusieurs milligrammes des protéines cœur de la voie NHEJ en cellules d’insecte à l’aide du système MultiBac. Nous avons ainsi purifié les complexes Ku70/Ku80 et Ligase4/XRCC4 et les protéines Cernunnos et Artemis à homogénéité. Des essais de cristallisation, des études par SAXS et des analyses par microscopie électronique ont été réalisés sur différents complexes formés par ces protéines cœur du NHEJ. Nous avons également caractérisé par chromatographie d’exclusion de taille et calorimétrie, les interactions effectuées entre les protéines de la voie NHEJ. L’ensemble de ces travaux a permis d’établir des bases biochimiques solides en vue des études structurales et fonctionnelles de la voie NHEJ chez l’homme. / Human DNA repair pathway NHEJ (Non-Homologous End-Joining) is a major pathway of double-strand breaks repair. The proteins involved in this pathway interact and form dynamic complexes whose molecular mechanisms are largely unknown. Firstly, we established protocols to be able to purify milligrams of those NHEJ pathway core proteins using MultiBac insect cells system. We then purified Ku70/Ku80 and Ligase4/XRCC4 complexes, Artemis and Cernunnos to homogeneity. Crystallogenesis assays, SAXS experiments and Transmission Electronic Microscopy experiments have been performed on several complexes formed by these core NHEJ proteins. We also characterized the interactions between these proteins by Size Exclusion Chromatography and Isothermal Calorimetry. These experiments have led to biochemical results sufficient to establish a solid basis to initiate the structural and functional study of the Human NHEJ Pathway.

Many different message-oriented middlewares (MOM) exist today on the market. With different underlying designs, purposes and features, it is not easy to make the right choice of MOM for your system. The IT company Nordicstation is in such a situation where they need to make a choice of MOM. They currently own a system called Sharelock which generates reports about violations of financial assets trad- ing. They wish to make the process of generating a report more asynchronous by using a MOM and they have a couple of requirements. This thesis was carried out with the purpose of finding the most suitable products for the system in mind, compare their features, performance, licenses and ease-of- use. This was accomplished by analysing their installation process, monitoring in- terfaces, documentation on their websites and performance in a simple throughput test. The results showed that RabbitMQ was the strongest candidate. It had good per- formance, an attractive web interface for monitoring, an easy installation and it also offered commercial support. Apache Artemis was also found to be an attractive choice but it did not have a web interface which made it hard to manage the system. / Idag finns det många meddelandebaserade mellanprogramvaror (MOM) på marknaden. Dessa har olika grundläggande designer, syften och kännetecken vilket kan göra det svårt att göra ett bra val av MOM för ett visst system. IT-företaget Nordicstation befinner sig i en sådan situation där de behöver göra ett val av MOM. De har ett system som kallas Sharelock som genererar rapporter om regelbrott in- om värdepappershandeln. De vill göra processen mer asynkron genom att använda en MOM och de har ett antal krav på produkten. Det här examensarbetet utfördes med syftet att hitta de mest passande produkter- na för det tänka systemet, jämföra deras särdrag, prestanda, licenser och anvä- ndarvänlighet. Detta gjordes genom att analysera deras installationsprocess, övervakningsgränssnitt, dokumentation på deras hemsida och prestanda i ett en- kelt prestandatest. Resultaten visade att RabbitMQ var den starkaste kandidaten. Den hade bra prestanda, ett attraktivt webbgränssnitt, en enkel installation och den erbjöd också kommersiell support åt kunder. Apache Artemis var också ett attraktivt val men den hade inget webbgränssnitt vilket gjorde det svårt att övervaka och hantera sys- temet.

DNA double-strand breaks (DSBs) containing unligatable termini are potent cytotoxic lesions leading to growth arrest or cell death. The Artemis nuclease and tyrosyl-DNA phosphodiesterase (TDP1) are each capable of resolving protruding 3′-phosphoglycolate (PG) termini of DNA double-strand breaks (DSBs). Consequently, a knockout of Artemis and a knockout/knockdown of TDP1 rendered cells sensitive to the radiomimetic agent neocarzinostatin (NCS), which induces 3′-PG-terminated DSBs. Unexpectedly, however, a knockdown or knockout of TDP1 in Artemis-null cells did not confer any greater sensitivity than either deficiency alone, indicating a strict epistasis between TDP1 and Artemis. Moreover, a deficiency in Artemis, but not TDP1, resulted in a fraction of unrepaired DSBs, which were assessed as 53BP1 foci. Conversely, a deficiency in TDP1, but not Artemis, resulted in a dramatic increase in dicentric chromosomes following NCS treatment. An inhibitor of DNA-dependent protein kinase, a key regulator of the classical nonhomologous end joining (C-NHEJ) pathway sensitized cells to NCS but eliminated the sensitizing effects of both TDP1 and Artemis deficiencies. Moreover, Polynucleotide Kinase/ Phosphatase (PNKP) is known to process 3′-phosphates and 5′-hydroxyls during DSB repair. PNKP-deficiency sensitized both HCT116 and HeLa cells to 3′-phosphate ended DSBs formed upon radiation and radiomimetic drug treatment. The increased cytotoxicity in the absence of PNKP was synonymous with persistent, un-rejoined 3′-phosphate-ended DSBs. However, DNA-PK deficiency sensitized PNKP-/- cells to low doses of NCS suggesting that, in the absence of PNKP, alternative enzyme(s) can remove 3′-phosphates in a DNA-PK-dependent manner. These results suggest that TDP1 and Artemis perform different functions in the repair of terminally blocked DSBs by the C-NHEJ pathway, and that whereas an Artemis deficiency prevents end joining of some DSBs, a TDP1 deficiency tends to promote DSB mis-joining. In addition, loss of PNKP significantly sensitizes cells to 3′-phosphate-ended DSBs due to a defect in 3′-dephosphorylation.

Thesis (Ph. D.)--Rheinische Friedrich-Wilhelms-Universität, Bonn, 1983. / Vita. Includes bibliographical references.

Evolution is a key distinguishing trait of Systems-of-Systems (SoS) that introduces a layer of complexity in analysis that is not present when considering static systems. Some SoS analysis tools exist to determine and evaluate the evolution of an SoS, while other tools are better suited for studying individual instances of an SoS. System Operational Dependency Analysis (SODA) is one such method that has been used previously to study static SoS networks. SODA that has been proven effective in investigating the impacts of partial system disruptions and would benefit from a framework to apply SODA to evolving SoS. This thesis provides an approach to modeling evolving SoS in SODA and presents new data visualization methods to highlight the effects of changing network configurations across evolutionary phases. These visualization enhancements include Failure Impact Range sequence plots to show effects of deterministic system disruptions on capabilities of interest across evolutionary phases, as well as Stochastic Impact plots to quantify the impact of disruptions in particular systems in the context of the probabilistic operating statuses assigned to each system. Integration of SODA and the related method of System Developmental Dependency Analysis (SDDA) is explored to model how operational disruptions and developmental delays might interact and compound during the evolution of an SoS. The SODA enhancements provide decision makers with new information that can be used to explore design and implementation tradeoffs in an evolving SoS under budget and scheduling constraints. These ideas are demonstrated through a case study based on NASA's Artemis program to return humans to the Moon in commercially-built Human Landing Systems (HLS). The HLS concepts proposed to NASA consist of multiple elements that provide distinct capabilities in different phases of the lunar mission, and therefore can be considered an evolving SoS architecture. The operational dependencies of two HLS concepts are modeled across a four-phase lunar landing mission and results are generated using the new visualization methods to highlight the impacts of changing SoS configuration on the performance of key mission capabilities. The development timeline of the first three planned Artemis lunar landing missions is analyzed with SDDA and integrated with SODA results from one HLS concept to explore how developmental delays impact the likelihood of HLS mission completion and how operational failures requiring system redesign impact the program schedule. Connections between SDDA and Integrated Master Schedules (IMS) are discussed to show how SDDA results can be useful in a context more familiar to program managers.

The purpose of the following research is to explore molten regolith electrolysis (MRE) methodology for in-situ resource utilization (ISRU) of Highlands lunar regolith, to be explored during the initial Artemis missions. An analysis of potential commercial launch providers for MRE-equipment based on technology-readiness level (TRL), payload mass support, and $ USD/kg payload price is provided. SpaceX is ultimately proposed as a launch provider of MRE equipment following multi-factorial analysis, with the SpaceX Starship human landing system (HLS) variant proposed for supporting MRE payload. Finally, customers of regolith-derived oxygen, aluminum, and silicon are distinguished to form the business case for operating MRE equipment on the lunar surface.

The Technische Universität Dresden has multiple e-learning projects in use. The Chair of Software Technology uses Inloop to teach students object-oriented programming through automatic feedback. In the last years, interest has grown in giving students automated feedback on modeling tasks. This is why there was an extension developed by Hamann to automate the assessment of modeling tasks in 2020. The TU Dresden currently has plans to replace Inloop with Artemis, a comparable system. Artemis currently supports the semi-automatic assessment of modeling exercises. In contrast, the system proposed by Hamann, called Inloom, is based on a rule-based approach and provides instant feedback. A rule-based system has certain advantages over a similarity-based system. One advantage is the mostly better feedback that these systems generate. To give instructors more flexibility and choice, this work tries to identify possible ways of extending Artemis with the rule-based approach Inloom. In the second step, this thesis will provide a proof of concept implementation. Furthermore, a comparison between different systems is developed to help instructors choose the best suitable system for their usecase.:Introduction, Background, Related Work, Analysis, System Design, Implementation, Evaluation, Conclusion and Future Work, Bibliography, Appendix