Three studies were designed to investigate the effects of exercise-based lifestyle interventions including diet change and exercise training on cardiovascular and autonomic responses to various physical and mental challenges in untrained premenopausal women. In Study 1, the effects of different tasks designed to activate the sympathetic nervous system on autonomic control of cardiovascular functioning such as the Stroop colour-word task (Stroop) and lower body negative pressure (LBNP) in 20 untrained premenopausal women (22.6??0.7 years) were determined. In Study 2, a longitudinal exercise intervention strategy was used with 18 untrained premenopausal women (22.5??0.7 years) in order to investigate the effect of 15 weeks of high intensity intermittent exercise (HIIE) training on cardiac autonomic responses to mental challenge (Stroop) and LBNP. In Study 3, the effects of HIIE training combined with a Mediterranean-style eating plan and fish oil supplement (Fish oil, Exercise, Mediterranean diet; FEM) on cardiovascular function during mental challenge (Stroop) and physical tasks (handgrip and reactive hyperaemia) were examined in 32 overweight untrained premenopausal women (22.0??0.6 years). In these studies, forearm blood flow (FBF) was assessed using Hokanson Plethysmography with the venous occlusion technique. The surface electrocardiogram and continuous beat-to-beat arterial blood pressure were also monitored. Peak oxygen uptake was assessed using open-circuit spirometry (True Max 2400, ParvoMedics). In addition, body composition was measured using DEXA (dual energy X-ray absorptiometry; DPX-IQ, Lunar Radiation). Results from Study 1 indicate that FBF response to mental challenge in young females was smaller compared to previously obtained data from age-matched males. Furthermore, this FBF response to mental challenge was negatively correlated to insulin resistance estimated by the homeostasis model assessment (HOMA-IR) (r = - .52, p < .05). In addition, when cardiopulmonary baroreceptors were unloaded by a mild level of LBNP (-20 mmHg) during Stoop, FBF response to mental challenge (vasodilation) was abolished suggesting a large dependency of vasodilation response during mental challenge on cardiopulmonary baroreflex. After 15 weeks of supervised HIIE training, aerobic fitness improved (p < .05) whereas percent of body fat was significantly decreased (p < .05). In addition, recovery BP following Stroop was significantly reduced. Insulin resistance (HOMA-IR) was marginally decreased (p = .056). Women who had higher insulin resistance (HOMA-IR) lost less fat than women with lower HOMA-IR (r = .60, p = .088). In addition, change in FBF during Stroop after training was directly related to pretest insulin resistance levels (r = .68, p < .05). Therefore, HIIE training had a normalising effect on FBF response to mental challenge. PEP/LVET ratio at rest and during LBNP was also significantly increased in women with higher HOMA-IR suggesting a reduction in cardiac contractility via a decrease in sympathetic stimulation (r = .62, p = .076, r = .62, p = .75 respectively). In Study 3 results indicated that 12 weeks of the FEM trial significantly reduced percent of body fat (p < .001), fasting insulin (p < .05), interleukin-6 (p < .05) and cortisol (p < .05), and significantly improved aerobic fitness ( ; p < .001). With respect to cardiovascular and cardiac autonomic measures, rate pressure product (RPP) was significantly reduced at rest (p < .05) and during recovery after Stoop (p < .05), suggesting decreased myocardial oxygen consumption. In addition, baseline heart rate determined in the sitting position was significantly reduced (p < .05), while both baseline high frequency power (HF) determined in supine (p < .01) and cardiac baroreflex sensitivity (BRS) determined in the sitting position (p < .05) were increased after the FEM trial. In addition, BRS determined during mental challenge also marginally increased (p = .051). In summary, lifestyle intervention including HIIE training, Mediterranean-style eating plan, and a fish oil supplement significantly enhanced parasympathetic influence of the heart and improved fitness, blood profiles, and body composition.

<p>MobileMe is a recently developed system for monitoring and recording physiological variables. It is wireless, and can therefore be suitable for field research, for example when measuring motion sickness symptoms.</p><p>The aim of this thesis was to conclude whether the MobileMe recording system was valid for research studies. A validation study, consisting of two parts and including 10 subjects, was performed. The first part was a laboratory study, where data from MobileMe and a reference equipment were compared. A field study was also performed, onboard a combat boat, to determine the equipment’s validity in uncontrolled environments. Furthermore, the field study included an investigation of motion sickness symptoms, and provided data for evaluation of motion sickness rating scales.</p><p>Statistical results from the laboratory study, and results from evaluation of data from the field study, showed that MobileMe was valid in both controlled and uncontrolled environments.</p>

Prader-Willi syndrome (PWS) is a rare, neurodevelopmental disorder with multiple features caused by hypothalamic deficiency, including infantile failure to thrive, hyperphagia leading to obesity, growth hormone deficiency, hypogonadism, and central adrenal insufficiency. Other features of PWS including global developmental delay, hypotonia, pain insensitivity, gastrointestinal dysfunction, and psychiatric disorders are caused by deficits in other regions of the nervous system. PWS is caused by the loss of a subset of paternally-expressed genes on chromosome 15, which includes NDN and MAGEL2. Necdin and Magel2 are both members of the melanoma antigen (MAGE) family of proteins and are expressed throughout development, particularly in the nervous system. This thesis describes experiments that examine the loss of function of necdin and Magel2 in mice and their potential roles in the pathogenesis of PWS. Targeted inactivation of Ndn and Magel2 in mice has aided in determining how loss of function of these proteins affects the development and function of the nervous system. Loss of necdin causes reduced axonal outgrowth and neuronal differentiation in the central and peripheral sensory nervous systems. I examined the autonomic nervous system in Ndn-null embryos and identified a defect in the migration of the most rostral sympathetic chain ganglion and consequently increased neuronal cell death and reduced innervation of target tissues supplied by this ganglion. Reduced axonal outgrowth was observed throughout the sympathetic nervous system in Ndn-null embryos although no gross deficits in the parasympathetic and enteric nervous systems were identified. Loss of Magel2 causes reduced fertility and abnormal circadian rhythm patterns in mice. I further identified an altered response to stress, a delayed response to insulin-induced hypoglycemia, a reduced stimulated growth hormone response, and lower thyroid hormone levels in Magel2-null mice, indicative of deficits in multiple hypothalamic-pituitary axes. The findings presented in this thesis support a role for necdin and Magel2 in the development and function of the nervous system. The data also indicates that these MAGE proteins play a key role in multiple features of PWS, including endocrine deficiencies and autonomic dysfunction

Large-scale deployment of IEEE 802.11 wireless LANs (WLANs) remains a significant challenge. Many access points (APs) must be deployed and interconnected without a-priori knowledge of the demand. We consider that the deployment should be iterative, as follows. At first, access points are deployed to achieve partial coverage. Then, usage statistics are collected while the network operates. Overloaded and under-utilized APs would be identified, giving the opportunity to relocate, add or remove APs. In this thesis, we propose extensions to the WLAN architecture that would make our vision of iterative deployment feasible. One line of work focuses on self-configuration, which deals with building a WLAN from APs deployed without planning, and coping with mismatches between offered load and available capacity. Self-configuration is considered at three levels. At the network level, we propose a new distribution system that forms a WLAN from a set of APs connected to different IP networks and supports AP auto-configuration, link-layer mobility, and sharing infrastructure between operators. At the inter-cell level, we design a load-balancing scheme for overlapping APs that increases the network throughput and reduces the cell delay by evenly distributing the load. We also suggest how to reduce the handoff time by early detection and fast active scanning. At the intra-cell level, we present a distributed admission control that protects cells against congestion by blocking stations whose MAC service time would be above a set threshold. Another line of work deals with self-deployment and investigates how the network can assist in improving its continuous deployment by identifying the reasons for low cell throughput. One reason may be poor radio conditions. A new performance figure, the Multi-Rate Performance Index, is introduced to measure the efficiency of radio channel usage. Our measurements show that it identifies cells affected by bad radio conditions. An additional reason may be limited performance of some AP models. We present a method to measure the upper bound of an AP’s throughput and its dependence on offered load and orientation. Another reason for low throughput may be excessive distance between users and APs. Accurate positioning of users in a WLAN would permit optimizing the location and number of APs. We analyze the limitations of the two most popular range estimation techniques when used in WLANs: received signal strength and time of arrival. We find that the latter could perform better but the technique is not feasible due to the low resolution of the frame timestamps in the WLAN cards. The combination of self-configuration and self-deployment enables the autonomic operation of WLANs.

There are numerous indications of possible involvement of the autonomic nervous system in the genesis of chronic pain. The possibility exists that sympathetic activation is related to motor dysfunction and changes in sensory processing, which have otherwise been implicated in musculoskeletal disorders. The primary aim of the thesis has been to investigate autonomic regulation at rest and in response to laboratory tests of autonomic function in subjects suffering from chronic pain in different localisations (lower back, neck-shoulder and neck-jaw), as well as to study the relations between autonomic regulation, proprioceptive acuity and clinical data. Secondary aim has been to assess autonomic regulation in fit, pain-free subjects in response to experimentally induced pain and in occupationally relevant settings. A total of 194 subjects suffering from chronic pain participated [low back pain (LBP) n=93; non-traumatic neck pain (NT) n=40, Whiplash associated disorder (WAD) n=40, Whiplash with temporomandibular dysfunction (WADj) n=21]. Each chronic pain group was subjected to a battery of autonomic function tests combining cognitive (Stroop Colour-Word conflict tests), physical (handgrip), sensory (unpleasant sound) and motor tasks (chewing tests) as well as the activation of reflex pathways (paced breathing and the orthostatic test) and compared to an age- and gender balanced control group. Autonomic regulation was also assessed in exposure to experimentally induced muscle pain in healthy subjects (n=24) in order to describe acute pain reaction. Further assessment was carried out during monotonous repetitive work and dynamic work in healthy subjects (n=10) and in a three-day monitoring of ambulance personnel (n=26) in occupational settings. Autonomic regulation was evaluated using cardiovascular (heart rate and heart rate variability, local blood flow and blood pressure), respiratory (breathing rate) electrodermal (skin conductance), muscular (trapezius and masseter EMG) and biochemical (insulin, cortisol, catecholamines) variables. Proprioceptive acuity was assessed using active-active repositioning tests. Pain levels were assessed using Visual-analogue or Numerical Rating scales. General health was evaluated through the Short-Form SF-36 Health Related Quality of Life questionnaire and Self-Efficacy Score questionnaires, whereas dysfunction was evaluated using the Oswestry Low Back Pain questionnaire, Pain Disability and Neck Disability Index questionnaires, the McKenzie evaluation and primary healthcare diagnoses. Self-reports of pain, stress and exertion were acquired prior to, during and post-testing. Chronic pain subjects were characterised by increased sympathetic and decreased parasympathetic activity as reflected in heart rate (LBP, WAD, WADj), heart rate variability (LBP, WAD, WADj), blood pressure (WADj) and electrodermal activity (LBP). In general, WAD showed more pain and dysfunction than NT, with lower self-efficacy and health-related quality of life. Differential reactivity was observed only in WAD, with increased responsiveness to sensory stimuli (heart rate variability, electrodermal activity), and motor tasks (heart rate) and a decreased response to cognitive challenge (heart rate variability, electrodermal activity). A significant part of WADj subjects showed sensorimotor impairment and low endurance in chewing tests, concomitant with a cardiovascular response that correlated with pain levels. Proprioceptive acuity was not found to be impaired among subjects suffering from chronic pain, and there were no indications of significant individual response specificity. Response to experimentally induced muscle pain in healthy subjects was also characterised by a prominent cardiovascular component. In simulated occupational settings autonomic activation and transient insulin resistance were detected in healthy subjects following monotonous repetitive work, with no similar effects following dynamic exercise. Modest deviations in circadian heart rate variability patterns during workdays were detected in ambulance personnel reporting more pronounced musculoskeletal symptoms, with no such effects on work-free days. Autonomic balance observed in chronic pain subjects was characterised by a trend towards increased sympathetic activity in comparison with pain-free controls. Moderate signs of affected reactivity to autonomic function tests were observed in patients with WAD, however no specific reaction patterns have been observed in any chronic pain group. Correspondence between the intensity of pain and autonomic activity was observed in acute pain and in chronic pain groups characterised by higher pain levels. As indicated by autonomic and neurohormonal changes in the recovery from real and simulated work, further studies with physiological monitoring of the effects of work-related stress are warranted for better understanding of the mechanism of musculoskeletal disorders.

This thesis investigates designs that enable individual components of a distributed system to work together and coordinate their actions towards a common goal. While the basic motivation for our research is to develop engineering principles for large-scale autonomous systems, we address the problem in the context of resource management in server clusters that provide web services. To this end, we have developed, implemented and evaluated a decentralized design for resource management that follows four principles. First, in order to facilitate scalability, each node has only partial knowledge of the system. Second, each node can adapt and change its role at runtime. Third, each node runs a number of local control mechanisms independently and asynchronously from its peers. Fourth, each node dynamically adapts its local configuration in order to optimize a global utility function. The design includes three fundamental building blocks: overlay construction, request routing and application placement. Overlay construction organizes the cluster nodes into a single dynamic overlay. Request routing directs service requests towards nodes with available resources. Application placement partitions the cluster resources between applications, and dynamically adjusts the allocation in response to changes in external load, node failures, etc. We have evaluated the design using complexity analysis, simulation and prototype implementation. Using complexity analysis and simulation, we have shown that the system is scalable, operates efficiently in steady state, quickly adapts to external events and allows for effective service differentiation by a system administrator. A prototype has been built using accepted technologies (Java, Tomcat) and evaluated using standard benchmarks (TPC-W and RUBiS). The evaluation results show that the behavior of the prototype matches closely that of the simulated design for key metrics related to adaptability and robustness, therefore validating our design and proving its feasibility. / QC 20100629

The presented studies were performed to investigate the effects of motion sickness (MS) on human autonomic and behavioural thermoregulatory mechanisms during cold stress and in a thermoneutral environment. The roles of histaminergic and cholinergic neuron systems in autonomic thermoregulation and MS-dependent dysfunction of autonomic thermoregulation were studied using a histamine-receptor blocker, dimenhydrinate (DMH), and a muscarine-receptor blocker, scopolamine (Scop). In addition, the effects of these substances on MS-induced nausea and perceptual thermoregulatory responses were studied. MS was found to lower core temperature, during cold stress by attenuation of cold-induced vasoconstriction and decreased shivering thermogenesis, and in a thermoneutral environment by inducing sweating and vasodilatation. The increased core cooling during cold stress was counteracted by DMH but not by Scop. In a thermoneutral environment, the temperature was perceived as uncomfortably warm during and after the MS provocation despite decreases in both core and skin temperature. No such effect was seen during cold-water immersion. Both pharmacologic substances had per se different effects on autonomic thermoregulatory responses during cold stress. Scop decreased heat preservation, but did not affect core cooling, while DMH reduced the rate of core cooling through increased shivering thermogenesis. Both DMH and Scop per se decreased thermal discomfort during cold-water immersion.Findings support the notion of modulating roles of histamine (H) and acetylcholine (Ach) in autonomic thermoregulation and during MS. MS activates cholinergic and histaminergic pathways, thereby increasing the levels of H and Ach in several neuro-anatomical structures. As a secondary effect, MS also elevates blood levels of several neuropeptides, which in turn would influence central and/or peripheral thermoregulatory responses.In conclusion, MS may predispose to hypothermia, by impairment of autonomic thermoregulation in both cold and thermoneutral environments and by modulation of behavioural thermoregulatory input signals. This might have significant implications for survival in maritime accidents. / <p>Medicine doktorsexamen</p>

Communication network technologies have been evolving exponentially in the late decades. These innovations increase the network capabilities and open new horizons to creating novel and original services. The heterogeneity in equipment qualifications increases the level of complexity in the technological advancement. In such environment, service management has become an everyday challenge to service providers. Important efforts have been deployed to innovate in the exploitation of intelligent devices in the home and other private locations. In this Thesis, we propose a framework for self-configuration of devices within Hone Area Networks. We propose a device self-configuration architecture based on IBM Monitor-Analyze-Plan-Execute using Knowledge autonomic control loop. To prove the validity of our system architecture and support its applicability, we developed a prototype system that gives a general control loop implementation for device self-configuration using the CPE WAN Management Protocol. A video streaming scenario is implemented and used to evaluate validity our framework.

This thesis presents an approach to the design and management of core networks where the packet transport is the main service and the backbone should be able to respond to unforeseen changes in network parameters in order to provide smooth and reliable service for the customers. Inspired by Darwin's seminal work describing the long-term processes in life, and with the help of graph theoretic metrics, in particular the "random-walk betweenness", we assign a survival value, the network criticality, to a communication network to quantify its robustness. We show that the random-walk betweenness of a node (link) consists of the product of two terms, a global measure which is fixed for all the nodes (links) and a local graph measure which is in fact the weight of the node (link). The network criticality is defined as the global part of the betweenness of a node (link). We show that the network criticality is a monotone decreasing, and strictly convex function of the weight matrix of the network graph. We argue that any communication network can be modeled as a topology that evolves based on survivability and performance requirements. The evolution should be in the direction of decreasing the network criticality, which in turn increases the network robustness. We use network criticality as the main control parameter and we propose a network management system, AutoNet, to guide the network evolution in real time. AutoNet consists of two autonomic loops, the slow loop to control the long-term evolution of robustness throughout the whole network, and the fast loop to account for short-term performance and robustness issues. We investigate the dynamics of network criticality and we develop a convex optimization problem to minimize the network criticality. We propose a network design procedure based on the optimization problem which can be used to develop the long-term autonomic loop for AutoNet. Furthermore, we use the properties of the duality gap of the optimization problem to develop traffic engineering methods to manage the transport of packets in a network. This provides for the short-term autonomic loop of AutoNet architecture. Network criticality can also be used to rank alternative networks based on their robustness to the unpredicted changes in network conditions. This can help find the best network structure under some pre-specified constraint to deal with robustness issues.

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