1 |
Molekular-zytogenetische Untersuchungen und Expressionsanalysen des Multiplen MyelomsGrandy, Isabell 05 December 2006 (has links) (PDF)
Durch die Kombination von SKY-, Array-CGH-, und Expressionsnanalysen wurden ausgewählte MM-Zelllinien auf Aberrationen hin untersucht und diese genauer analysiert. 32 Myelom-Patienten wurden mittels Array-CGH-Analyse untersucht und aufgrund ihrer Aberrationen und der klinischen Daten durch eine anschließende Clusteranalyse in 4 Subgruppen unterteilt.
|
2 |
Molekular-zytogenetische Untersuchungen und Expressionsanalysen des Multiplen MyelomsGrandy, Isabell 30 November 2006 (has links)
Durch die Kombination von SKY-, Array-CGH-, und Expressionsnanalysen wurden ausgewählte MM-Zelllinien auf Aberrationen hin untersucht und diese genauer analysiert. 32 Myelom-Patienten wurden mittels Array-CGH-Analyse untersucht und aufgrund ihrer Aberrationen und der klinischen Daten durch eine anschließende Clusteranalyse in 4 Subgruppen unterteilt.
|
3 |
Synthesis and Biological Evaluation of Various Derivatives of a Broad-Spectrum Anticancer NucleosideShelton, Jadd R. 07 August 2012 (has links) (PDF)
Recently the Peterson lab discovered a promising anticancer adenosine derivative-- 2´,3´-bis-O-tert-butyldimethylsilyl-5´-deoxy-5´-[N-(methylcarbamoyl)amino]-N6-(N-phenylcarbamoyl)adenosine. This compound showed selective toxicity against human colon cancer cells in vitro with LC50's = 6--10 µM. It was hypothesized that the lead compound exerted its cytotoxic effects by interacting with a protein kinase. A systematic Structure Activity Relationship (SAR) was undertaken in an attempt to increase the kinase-binding affinity of the lead compound. Many regions of the lead compound were examined: the N6-phenyl urea moiety, the 5´-N-methyl urea group, the 2´,3´-bis-O-TBS groups, the nucleobase, and the ribose sugar. Results of these studies produced some promising new derivatives. In particular, one analogue exhibited potent cancer cell growth inhibition with an average GI50 of 0.58 μM (NCI-60). In addition, another compound showed selective toxicity for the non-small cell adenocarcinoma cell line NCI-H522 with an LC50 of 10 nM. Efficient methods for the preparation of a wide variety of N6-aryl and -alkyl substituted derivatives were developed. One versatile route involved the installation of an N6-ethoxy carbonyl and subsequent displacement with an alkly- or arylamine. Synthetic routes for the preparation of of a variety of 2´,3´-bis-O-acylated analogues were also developed. Nucleoside mono-, di-, and triphosphate bioisosteres in which the phosphoester or phosphoanhydride have been replaced by an unnatural functional group have been extensively investigated. A simple and efficient method was developed for the preparation of carbamoyl analogues of nucleoside mono-, di-, and triphosphate surrogates. This method uses a modified version of the Kočovský reaction to install mono-, di-, and triphosphate mimics in good to excellent yields (ave = 75%).
|
4 |
The Genetic Heterogeneity of Brachydactyly Type A1: Identifying the Molecular PathwaysRacacho, Lemuel Jean January 2015 (has links)
Brachydactyly type A1 (BDA1) is a rare autosomal dominant trait characterized by the shortening of the middle phalanges of digits 2-5 and of the proximal phalange of digit 1 in both hands and feet. Many of the brachymesophalangies including BDA1 have been associated with genetic perturbations along the BMP-SMAD signaling pathway. The goal of this thesis is to identify the molecular pathways that are associated with the BDA1 phenotype through the genetic assessment of BDA1-affected families. We identified four missense mutations that are clustered with other reported BDA1 mutations in the central region of the N-terminal signaling peptide of IHH. We also identified a missense mutation in GDF5 cosegregating with a semi-dominant form of BDA1. In two families we reported two novel BDA1-associated sequence variants in BMPR1B, the gene which codes for the receptor of GDF5. In 2002, we reported a BDA1 trait linked to chromosome 5p13.3 in a Canadian kindred (BDA1B; MIM %607004) but we did not discover a BDA1-causal variant in any of the protein coding genes within the 2.8 Mb critical region. To provide a higher sensitivity of detection, we performed a targeted enrichment of the BDA1B locus followed by high-throughput sequencing. We report the identification of a novel 9.5 Kb intergenic tandem duplication in two unrelated BDA1-affected families. In-vitro and in-vivo reporter assays demonstrated the enhancer activity of noncoding conserved sequence elements found within the microduplication. We also show an upregulation of the neighboring genes, NPR3 and PDZD2, in the patients' fibroblasts that suggests a gain-of-function through the duplication of cis-regulatory elements on dose sensitive genes. By expanding the repertoire of BDA1-causing mutations in IHH, GDF5, BMPR1B and at the BDA1B locus, we have begun to elucidate a common genetic pathway underlying phalangeal formation and elongation.
|
Page generated in 0.0121 seconds