The amphibian species Xenopus laevis, along with mouse and chicken is a very important model system, used widely to dissect the molecular intricacies of various aspects of vertebrate development. Study with Xenopus has clear advantages in terms of various technical considerations including the ease of handling early stage of embryos and due to the remarkable documentation of several early molecular events during development. The concept of inductive interactions between various cell types during early development was first revealed by the studies performed in Xenopus, and among the various factors proposed for mesoderm induction, the members of transforming growth factor-β (TGF- β) superfamily have been considered to be the most probable candidates. About forty different members of the TGF-β superfamily have been cloned and characterized from various organisms. The superfamily members like activins and BMPs have been studied extensively with respect to their functional role during development. While BMPs were assigned as candidates for inducing ventral mesoderm, activins oppose the role of BMPs by inducing dorsal mesoderm. Studies that helped in delineating their roles were performed using three approaches that utilized the ligands, receptors or down stream signaling components (Smads). All the three components were studied with respect to their endogenous expression pattern and effects of ectopic expressions of the wild type or dominant negative mutants. These approaches led to the accumulation of evidences supporting the importance of these signaling molecules. All the above mentioned studies were only possible due to the cloning and characterization of cDNAs of the various proteins involved in the signaling pathway including the ligands. TGF-β2 and 5 are the two isoforms of TGF-β cloned from the amphibian system. We have earlier cloned and characterized the promoter for TGF-β5 gene, which suggested possible regulation of this factor by tissue specific transcription factors. Messenger RNA in situ hybridization analysis to study the TGF-β5-expression pattern during Xenopus development, showed spatial and temporal expression pattern. The expression was confined to specific regions that include notochord, somites, and tail bud among others, in the various stages analyzed. This suggested a possible role for TGF-β5 in organogenesis during the amphibian development. To better understand the role of TGF-β in Xenopus development, studies to examine the specific receptor expression pattern for this growth factor is very essential. With the lack of any reports on cloning of TGF-β receptors from this system, the aim of the present study was to isolate and characterize the receptors for TGF-β from Xenopus laevis. PCR cloning using degenerate primers based on the conserved kinase domains of this class of receptors, coupled to library screenings enabled the identification of two novel receptor cDNAs of the TGF-β receptor superfamily. Characterization of the isolated cDNAs suggested that one of them codes for a type II receptor for TGF-β. Further the cDNAs were found to be ubiquitously expressed during development, as judged by RT-PCR analysis. The cloned cDNAs can now be employed as tools, to study the expression pattern by means of mRNA in situ hybridization, on the various developmental stage embryos and to perform studies using antisense and dominant negative mRNA injection experiments in vivo. Such studies will greatly assist in delineating the role of TGF-β ligands and receptors during amphibian development.

Hydrology and water quality are affected by land use and climate changes. Mississippi’s diverse agro–ecosystem comprises of a range of land use land cover (LULC) including agriculture, forest, wetlands, urban, and grasslands. The objectives of this study were to investigate the impacts of various factors such as Best Management Practices (BMPs), wetlands, LULC, and climate changes on water quality and quantity. The hydrologic and water quality responses to dynamic LULC input in Soil and Water Assessment Tool (SWAT) were evaluated. Results showed that agricultural and forest expansion were major drivers of hydrologic and water quality changes in Big Sunflower River Watershed (BSRW), with agricultural expansion increasing runoff, sediments, and nutrients and forest expansion reducing these variables. The results showed that the integration of dynamic LULC and agricultural management operations in SWAT enables a more realistic representation of agricultural watersheds. Similarly, this study investigated the effects of wetland area changes overtime on surface and groundwater. Results demonstrated that 26% increase in wetland areas, reduced streamflow, sediments, total nitrogen, and total phosphorus by 2%, 37%, 13%, and 4% respectively as well as increased groundwater storage by 90 mm in selected sub–watershed. This highlighted the importance of preservation and restoration of wetlands to enhance the agro–ecosystem resilience to LULC change. Likewise, the effectiveness of BMPs in reducing sediment yield from critical areas within BSRW was assessed. Results demonstrated that BMPs reduced sediments by up to 50%, suggesting their usefulness in mitigating high sediment yield from agricultural areas. This study also assessed the impacts of climate change on streamflow and sediment loads and the role of waterbodies in mitigating those impacts. Results depicted a significant increase in future streamflow and sediment loads due to potential increase in precipitation and temperature. When waterbodies were simulated, projected change in annual streamflow was < 1%. However, the projected annual sediment loads reduced substantially by 44–46%, highlighting the role of waterbodies on watershed resilience to climate change. Overall, this dissertation study provides insights about the complex interactions between LULC, climate, anthropogenic activities, and water resources that can help to develop watershed management strategies to promote agricultural sustainability.

This study evaluates the spatial and temporal impacts of implemented Best Management Practices (BMP) on stream water quality under baseflow conditions in a stream segment affected by livestock production. The BMPs includes 10-m fenced riparian zone and crossing paths along the reach segment. Grab water samples and water quality monitoring were collected biweekly from July 2019 to March 2022. Water quality parameters included temperature, dissolved oxygen, electric conductance, total dissolved solids, turbidity, and pH. Water samples were lab analyzed for sediments and nutrients. Results evidenced poor water quality before BMPs implementation, with nutrient concentrations exceeding the nation's and state's criteria. Two years after implementation, BMPs favored seasonal and spatial reductions in nutrient, sediment concentrations, and improved water quality parameters. Present results and subsequent stream monitoring should create awareness in private owners to extend the implementation of a riparian zone and other BMPs that improves stream water quality and health.

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