The nutritional quality and antioxidant activity of organic and conventional green beans were compared in a series of experiments. Firmness, percent dry matter, carotenoids and chlorophyll contents, respiration rate, vitamin C and proteins were evaluated. Hydroxyl radical scavenging activity, SOD, APX and POX were also analysed. In a second experiment, organic green beans treated with hexanal formulations were stored with untreated at 12⁰C in the refrigerator for a period of three weeks during two seasons. The results of the experiment showed higher levels of most of the parameters measured in organic green beans (with significant differences in firmness, carotenoids and chlorophyll b), except for respiration, vitamin C, antioxidant activity(hydroxyl radical scavenging) and APX where conventional green beans were higher and showed significant differences in respiration and vitamin C. The storage experiment showed a decrease in firmness and fresh weight and increase in respiration during storage for both seasons. Carotenoids and chlorophyll contents showed decrease in concentrations during the first week and increased during the second week of storage.

Dry beans (Phaseolus vulgaris L.) contain high levels of folates. These compounds are essential vitamins and folate deficiencies may lead to a number of health problems. The objectives of this study were to examine the mode of inheritance of folate content and identify quantitative trait loci (QTL) associated with folate content in dry beans. Inheritance of folate content was studied in the F1 hybrids of one-way diallel crosses among Othello, AC Elk, Redhawk and Taylor, and an F2 population of the cross between Redhawk and Othello. Total folate content and 5 methyltetrahydrofolate (5MTHF) were measured twice within a one hour interval. Significant variation in folate content was observed among the parental genotypes, their F1 hybrids, and the F2 individuals of a cross between Redhawk and Othello, ranging from 147 to 345 µg/100g. Reductions in the 5MTHF content and total folate content values in the second measurement from samples were highly variable for all four parental lines ranging from 5 to 30% and 7 to 33%, respectively. A single marker QTL analysis identified at least three QTL for folate content in the F2 population. For the majority of identified QTL, dominance effects appeared to be the major genetic effect.

This work was devoted to the study of (a) the physico-chemical, functional, and structural properties of bean (Phaseolus sp.) protein isolates in relation to their microstructures, and (b) the effects of protein carbohydrate interactions on physico-chemical, functional, and structural properties. The contents of protein, and both total and individual sugars of alkali extracted (amorphous) and citric acid extracted (bipyramidal and spheroidal) proteins from Phaseolus vulgaris (white kidney and navy) and Phaseolus lutanus (baby lima and large lima) beans were determined. The proteins were subjected to differential scanning calorimetry, and measurements of surface tension (air-water interface), surface hydrophobicity, and foam expansion. Structural analysis of the proteins were performed using Fourier transform infrared (FT-IR) spectroscopy. Enzymatic and chemical deglycosylation was performed on a white kidney bean protein isolate. / Glucose and mannose were the major sugars found in the isolates. Bipyramidal and spheroidal microstructures with higher protein contents generally had greater mannose content and lower glucose content. Differences in enthalpy of denaturation $( Delta$H), surface tension decay curves, surface hydrophobicities, and foam expansions were observed with isolates of different microstructures. Corresponding differences in molecular structure were not, however, detected by FT-IR spectroscopy. Using statistical analysis, a relationship between foam expansion and the $ Delta$H, solubility, surface hydrophobicity and surface tension of the isolates was obtained. Preliminary results suggest that the removal of carbohydrate influenced the physico-chemical properties of the protein.

No description available.

No description available.

No description available.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1949. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves [38]-39).

Konstanz, FH, Diplomarb., 2003.

Made available in DSpace on 2014-06-11T19:28:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-06Bitstream added on 2014-06-13T18:58:00Z : No. of bitstreams: 1 sartori_je_me_botfca.pdf: 257358 bytes, checksum: b22cbf58843b34a23ad0f632bb89f4e0 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Colletotrichum lindemuthianum (Sacc. & Magn.) Scrib. e o agente causal da antracnose do feijoeiro (Phaseolus vulgaris L.), doenca fungica de grande importancia para a cultura, pois pode causar perdas de ate 100% na producao, quando sao utilizadas cultivares suscetiveis e sementes infectadas, sob condicoes ambientais favoraveis ao desenvolvimento de epidemias. O presente trabalho objetivou avaliar a sensibilidade in vitro de 20 isolados de Colletotrichum lindemuthianum, provenientes de diferentes regioes do pais, a cinco fungicidas de diferentes principios ativos e algumas misturas (carbendazin, chlorothalonil, tiofanato metilico, chlorothalonil + tiofanato metilico, trifloxystrobin, propiconazol, trifloxystrobin + propiconazol), em cinco diferentes concentracoes (0, 1, 10, 100 e 1000 -Êg.mL-1) adicionados em meio de cultura batata-dextrose-agar (BDA). Os isolados cresceram por sete dias a 25 -C no meio de cultura BDA e apos esse periodo, discos de 0,5 cm de micelio do fungo, foram transferidos para placas de Petri com o meio BDA contendo as diferentes concentracoes de fungicidas. As placas foram incubadas por sete dias a 25 -C, avaliado o crescimento medio do diametro das colonias (em cm) e calculado os valores de porcentagem de inibicao do crescimento micelial dos isolados em relacao ao tratamento testemunha. Os resultados mostraram que para os fungicidas carbendazin e tiofanato metilico, 11 isolados fungicos foram totalmente ou parcialmente inibidos na concentracao de 1 ou 10 -Êg.mL-1. Chlorothalonil mostrou-se menos eficiente que os demais produtos pelo fato de inibir o crescimento micelial de apenas tres isolados na concentracao de 1000 -Êg.mL-1. A mistura de chlorothalonil e tiofanato metilico mostrou a inibicao total do crescimento micelial de quatro isolados a 1 -Êg.mL-1, e, a 1000 -Êg.mL-1, oito isolados mostraram-se sensiveis a mistura desses produtos... / Colletotrichum lindemuthianum (Sacc. & Magn.) Scrib. is the causal agent of anthracnose in bean (Phaseolus vulgaris L.). This fungal disease has great importance in the crop, since it may cause yield losses of up to 100% when susceptible cultivars and infected seeds are used under environmental conditions that favor the development of epidemics. The aim of this work was to evaluate the in vitro sensitivity of 20 isolates of Colletotrichum lindemuthianum from different regions of the country, to five fungicides with different active principles and some mixtures (carbendazim, chlorothalonil, methyl thyophanate, chlorothalonil + methyl thyophanate, trifloxystrobin, propiconazole, trifloxystrobin + propiconazole), at five different concentrations (0, 1, 10, 100, and 1000 ìg.mL-1), added to potato-dextrose-agar (PDA) culture medium. The isolates were cultured for seven days at 25 °C in PDA medium, after which 0.5 cm disks of fungal mycelium were transferred to Petri dishes containing PDA medium and the various concentrations of fungicides. The Petri dishes were incubated for seven days at 25 °C. We evaluated the mean growth in diameter of the colonies (in cm) and calculated percentage values for mycelial growth inhibition of the isolates in relation to the control treatment. The results showed that 11 fungal isolates were completely or partially inhibited by the fungicides carbendazim and methyl thyophanate at concentrations of 1 or 10 ìg.mL-1. Chlorothalonil proved less effective than the other products, as it inhibited mycelial growth in only three isolates, at the concentration of 1000 ìg.mL-1. The chlorothalonil and methyl thyophanate mixture showed complete mycelial growth inhibition in four isolates at 1 ìg.mL-1, while eight isolates were sensitive to the mixture of these products at 1000 ìg.mL-1. The propiconazole and trifloxystrobin mixture was the most effective and inhibited growth... (Complete abstract click electronic access below)

Made available in DSpace on 2014-06-11T19:28:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-06-30Bitstream added on 2014-06-13T20:58:40Z : No. of bitstreams: 1 souzafilho_rc_me_botfca.pdf: 396976 bytes, checksum: b288af0202ba8273f1c200d274db6e8f (MD5) / O feijão-vagem (Phaseolus vulgaris L.) pertence à mesma família e espécie botânica do feijão comum que é cultivado no Brasil. O feijão-vagem é cultivado em cerca de 100 países, envolvendo grande número de gêneros e espécies. A antracnose incitada pelo fungo Colletotrichum lindemuthianum (Sacc. & Magn.) é uma das doenças mais importantes dessa cultura, pois causa severos danos. O presente trabalho teve por objetivo avaliar a sensibilidade in vitro e in vivo de 12 isolados de C. lindemuthianum de feijão-vagem, oriundos de diversas localidades situadas no Estado de São Paulo, a fungicidas, bem como determinar as raças fisiológicas desse fungo. A sensibilidade a fungicidas in vitro foi determinada através da aferição do crescimento micelial (12 isolados) e da germinação de conídios (4 isolados) em 5 concentrações (0, 1, 10, 100, 1000μg.mL-1) dos produtos oxicloreto de cobre + mancozeb, chlorotalonil, chlorotalonil + tiofanato metílico, tiofanato metílico, mancozeb, metiram + piraclostrobina, piraclostrobina, óxido cuproso e carbendazim foram adicionados em meio batata - dextrose - ágar (BDA) e determinadas as faixas de ED50. Para a avaliação in vivo, folhas primárias de feijão-vagem cultivar Itatiba II foram imersas nas soluções dos fungicidas oxicloreto de cobre + mancozeb (2g/L), chlorotalonil (2g/L), chlorothalonil + tiofanato metílico (3,5g/L), tiofanato metílico (2g/L), mancozeb (4g/L), metiram + piraclostrobina (3g/L), piraclostrobina (0,6ml/L), óxido cuproso (2g/L) e carbendazim (2ml/L), inoculadas separadamente com conídios dos quatro isolados de C. lindemuthianum, mantidas em placas de Petri, sob condições controladas e os... / The snap bean (Phaseolus vulgaris L.) belongs to the same family and botanical species of common bean that is grown in Brazil. It is a crop planted in about 100 countries around the world, involving a large number of genera and species. The anthracnose incited by Colletotrichum lindemuthianum (Sacc. & Magn.) is one of the most important diseases of this crop, it causes severe damage. This study aimed to evaluate the sensitivity in vitro and in vivo to fungicides of 12 isolates of C. lindemuthianum from snap beans of various localities in the state of São Paulo, as well as determine the races of this fungus. The sensitivity to fungicides in vitro was determined by measuring the mycelial growth (12 isolates) and spore germination (4 isolates) in 5 concentrations (0, 1, 10, 100 and 1000μg.mL-1) of fungicides oxychloride copper + mancozeb, chlorothalonil, chlorothalonil + methyl thiophanate, methyl thiophanate, mancozeb, metiram + pyraclostrobin, pyraclostrobin, oxide copper and carbendazim were added in potatodextrose- agar (PDA) and the ED50 interval were determineted. For in vivo evaluation, primary leaves of snap bean cultivar Itatiba II were immersed in solutions of the fungicides mancozeb + copper oxychloride (2g / L), chlorothalonil (2g / L), chlorothalonil + thiophanate methyl (3.5 g / L) , thiophanate-methyl (2g / L), mancozeb (4g / l), metiram + pyraclostrobin (3g / L), pyraclostrobin (0.6 mL / L), cuprous oxide (2 g / L) and carbendazim (2ml / l), conidia of the four isolates of C. lindemuthianum were inoculated separately and kept in petri dishes under controlled conditions. Symptoms of the disease were assessed with a diagrammatic severity. The results showed that the isolates of C. lindemuthianum from snap-beans presented low sensitivity to fungicides on mycelial growth of cooper oxide, mancozeb... (Complete abstract click electronic access below)