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In vitro and in vivo genetic stability of the rabies ERA glycoprotein expression cassette of AdRG13, a recombinant adenovirus vaccine against rabiesRoberts, Danielle Renee January 2005 (has links)
The genetic stability of the rabies glycoprotein (G) expression cassette of AdRG1.3 was examined using both in vitro and in vivo models. For the in vitro study, the AdRG1.3 vaccine was serially passaged 20 times in 293 cell culture and from the twentieth passage a total of 67 AdRG1.3 virus clones were obtained. The G gene expression cassette (including an SV40 polyadenylation signal sequence) along with flanking human adenovirus type 5 sequences were amplified from these clones using PCR to generate an amplified product approximately 2.25 kb long. These products were then purified and subjected to DNA sequence analysis. No changes were observed in the 1870 nucleotide sequence window (containing both the G gene (1572 nt) and the SV40 polyA sequence (132 nt)) of any of the 67 clones.
Findings show that the G gene insert is stably expressed in a conformationally appropriate form by the recombinant HAd5 vector.
The genetic stability of the G gene cassette of AdRG1.3 was also evaluated upon in vivo passage using five independent series of cotton rats (Sigmodon hispidus). From the fifth in vivo passage of AdRG1.3, a total of 105 virus clones were obtained from these five independent series of animals. The complete G gene expression cassette was amplified from these clones by PCR and sequenced as for the in vitro study; no base changes were observed in the targeted 1870 nucleotide sequence window of any of these clones. Therefore, these results show that the G gene expression cassette of AdRG1.3 remains stable within the adenovirus vector upon passage of the vaccine both in cell culture and in animals. (Abstract shortened by UMI.)
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Persistent organic pollutants in white-tailed deer ( Odocoileus virginianus) near a magnesium smelter: Spatial distribution and human health risk assessmentTolley, Cecilia A January 2006 (has links)
A magnesium refining facility in Quebec was a known point source of persistent organic pollutants namely polychlorinated biphenyls, dioxins and furans, and hexachlorobenzene. Contaminants concentrations were measured in the fat tissue of local white-tailed deer with the assistance of the local community and hunters. Concentrations in the deer from 1999, one year before the smelter opened, were compared with deer from 2002. Results showed a significant increase in PCBs from 1999 to 2002, and total PCB concentrations showed significant decreases with distance from the smelter. Many of the mid-range PCB homologues that bioconcentrate sharply in deer showed similar relationships. Sigmacoplanar PCBs and Cytochrome P450 1A expression in liver also showed a significant inverse relationship with distance in 2002. Results of a human health risk assessment indicated that the number of deer meal portions required to exceed safe consumption levels for PCDD/Fs and coplanar PCBs increased with the distance from the smelter.
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A comparative study of the anatomy and physiology of Erolia, Vieillot, and Ereunetes, Illiger (aves: Charadriiformes: Scolopacidae)January 1965 (has links)
acase@tulane.edu
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Zoogeography and systematics of isopoda of the Beaufort SeaBray, Joseph Russell January 1962 (has links)
Original print version missing pages 130-133 (appendix B and Appendix C)
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ECOSYSTEM RESPONSE TO BENTHIC DERIVED NUTRIENT SUBSIDIES FROM OMNIVOROUS FISHGlaholt, Stephen P., Jr 07 July 2003 (has links)
No description available.
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THE EFFECTS OF TEMPERATURE, BODY SIZE, AND GROWTH ON THE LOCOMOTOR PERFORMANCE OF JUVENILE TURTLESElnitsky, Michael Adam 30 November 2004 (has links)
No description available.
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Behaviorial Interactions Between Juvenile Stages of Yellow Perch and Round Goby Affects CompetitionDuncan, Janelle M. 16 August 2006 (has links)
No description available.
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Honors Thesis: BRCA1 Interactions with BACH1, BARD1, and CHK2: Recent Evidence and Potential Developments in the Diagnosis, Treatment, and Prevention of Human Breast CancerRice, Ian S. 25 April 2005 (has links)
No description available.
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Comparative Studies in the Functional Morphology of Lizard Feeding: Kinematics, Behavior, and BiomechanicsMcBrayer, Lance D. 12 November 2002 (has links)
No description available.
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Locomotion, Morphology, and Habitat use in Arboreal Squirrels (Rodentia: Sciuridea)Essner, Richard L., Jr. January 2003 (has links)
No description available.
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