Thesis (Ph. D.)--Swedish University of Agricultural Sciences, 2001. / Includes bibliographical references.

Thesis (M.S.)--Rutgers University, 2009. / "Graduate Program in Food Science." Includes bibliographical references (p. 53-56).

Darbo tikslas: ištirti juodųjų serbentų (Ribes nigrum L.) produktų, pasižyminčių antioksidantinėmis savybėmis įtaką triušių virškinimo procesams ir produktyvumui. Darbo tikslas įgyvendintas sprendžiant šiuos uždavinius: 1) aukštos apykaitynės energijos gautos iš padidinto riebalų kiekio racionų sudarymas, žolės miltus pakeičiant juodųjų serbentų išspaudomis; 2) nustatyti triušių kūno svorio, priesvorio, pašarų sąnaudų su juodaisiais serbentais sąveiką; 3) ištirti juodųjų serbentų išspaudų įtaką atskirų virškinimo trakto segmentų turinio pH ir sausųjų medžiagų kiekiui;4) nustatyti juodųjų serbentų išspaudų įtaką klubinės žarnos turinio klampumui, aklosios žarnos turinio amoniako kiekiui; 5) ištirti trumpųjų grandinių riebalų rūgščių (TGRR) koncentraciją ir sudėtį aklojoje žarnoje; 6) nustatyti juodųjų serbentų išspaudų įtaką aklosios ir gaubtinės žarnų turinio baltymų, bakterijų fermentiniams (α- ir β-galaktozidaziniams, gliukozidaziniams, α- ir β-gliukozidaziniams ir β-gliukuronidazidaziniams) aktyvumams ir lakiųjų riebalų rūgščių (LRR) kiekiams; 7) nustatyt ipoveikį triušių antioksidantinio aktyvumo rodikliams; 8) nustatyti poveikį biocheminiams kraujo rodikliams; 9) ištirti triušių aterogeniškumo ir atsparumo insulinui rodiklius. Išvados: 1) Juodųjų serbentų išspaudų naudojimas pakeičiant žolės miltus, patvirtino hipotezę, kad jos gali būti laikomos veiksminga ir prieinama pašarų sudedamąją dalimi triušių racionuose. 2) Raciono papildymas juodųjų serbentų išspaudomis... [toliau žr. visą tekstą] / Researches were conducted in the Animal Husbandry Department of the Veterinary Academy of Lithuanian Health Sciences University, also in the vivarium of Lithuanian Veterinary Academy and in the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences. New Zealand rabbits were used for the researches. All rabbits were calved over one cycle of reproduction (50 percent were male and 50 percent were female). All rabbits were assigned to four groups of five individuals each. The rabbits of the control group (K) were fed a standard diet; the group KP was fed a standard diet with increased fat volume. The rabbits of the group E were subjected to a standard diet with the addition of blackcurrant polyphenolic extract (1,5 percent), the animals of the group EP were fed a standard diet with increased fat volume + blackcurrant extract (1,5 percent). Duration of the research was 45 days. Rabbits were stored in the individual flat-bottomed wire cages in closed room. Over the research all rabbits were fed and watered ad libitum. The research included observation of rabbit growth dynamics, level of ammonia in the substance of cecum, activity of microbe ferments, short-chain fatty acids, and research of overall wellness. Thegoalofthepaper: to investigate the effect of blackcurrant (Ribes nigrum L.) products distinguishing for antioxidant features on digestive processes and productivity of rabbits. The goal of the paper has been achieved by solving the following... [to full text]

Growing numbers of morbidity and mortality due to the cancer and coronary heart disease is recognized as the increasing challenge in the world. The initial stage of these diseases, early diagnosis and treatment are the main objectives of current researches. According to Lithuanian Health Information Centre morbidity and mortality due to these diseases in Lithuania exceed average of European Union. The oxidative stress occurs in cells as a result of normal physiological processes and environmental interactions, and the complex antioxidant defense system are of particular importance in protecting biological systems against oxidative damage by free radicals. Recently polyphenols and anthocyanins containing foods and preparations have attracted attention due to their health promoting benefits. Anthocyanins make one of the main groups of flavonoids, and represent polyphenolic compounds, that are found in vegetables and fruits, particularly in berries. An important effect of anthocyanins is the scavenging of oxygen-derived free radicals. They can interfere with few different free radical-producing systems, and can increase the function of the endogenous antioxidants. The aim of the research is to choose sources for the products with antioxidant activity from plants cultivated in Lithuania, evaluate quality and antioxidant activity of black currant, red currant and chokeberry products, to develop and evaluate technologies of phytopharmaceuticals with antioxidant activity... [to full text]

The work deals with the chemical analysis of juices selected varieties of black currant (Ribes nigrum L.). The theoretical part is divided into three main chapters. In the first chapter there are short botanical characteristics of black currants, content of biologically active substances in fruit and utilization of black currant fruit in food industry. In the second chapter there are characteristics, importance and methods of determination of phenolic compounds. Vitamin C is describe in the last chapter, its characteristics, importance and methods of determination. Experimental part describes determination of three chemical characteristic of black currant fruits: content of total phenolic compounds, content of total anthocyanins and content of vitamin C. Twelve modern varieties of black currant were selected for determination. Based on these results, it is possible to deduce the more important varieties. The content of total phenolic compounds was in the range of 326,7–641,4 mg•100 g-1 in all samples. The content of total anthocyanins was analysed in the range from 102,5 to 284,5 mg•100 g-1 in all samples. And content of vitamin C was in the range from 82,0 to 379,1 mg•100 g-1 in all samples. The overall comparison of the varieties of black currant was found, the varieties Ceres, Ben Hope, Ben Lomond, Démon and Ometa are the most promising varieties in terms of the content of the above mentioned compounds.

The allergic disease of asthma is characterized by an infiltration of inflammatory cells to the lung, a process co-ordinated by T-helper (TH) cells. The TH2 cytokine Interleukin (IL)-4 promotes infiltration of eosinophils to sites of inflammation. Eosinophil-selective chemoattractant cytokines (eg. eotaxins) are synthesized by lung epithelial cells. Eotaxin-3 is expressed at high levels in the asthmatic lung, predominantly after IL-4 stimulation. Eotaxin-3 is therefore a marker of inappropriate airway inflammation. Polyphenolic (PP) compounds found in high concentrations in berries may have beneficial effects in inflammatory conditions. Plant and Food Research produced high-PP extracts of blackcurrant (BC) cultivars that were tested for inflammation modulating effects. Since high doses of PPs have been shown to cause cell death, we tested two BC cultivars at a range of concentrations in a cell viability (WST-1) assay. While no toxic effects were attributable to the BC extracts (1-50µg/ml), a dose-related trend in cell death was observed and therefore 10µg/ml was chosen for further experiments Ten BC cultivars were compared for efficacy by measuring eotaxin-3 production in IL-4 stimulated human lung epithelial (A549) cells in vitro. Cells were incubated with BC extracts (10µg/ml) and IL-4 (10ng/ml) for 24 hours. The supernatants were then quantified for eotaxin-3 levels by an enzyme-linked immunosorbent assay (ELISA). All ten BC extracts reduced eotaxin-3 levels after stimulation with IL-4, and six BC extracts were effective by statistically significant levels (P<0.05), (BC cultivars -01, -02, -03, -05, -09 & -10). Of those, BC extracts of four cultivars demonstrated a reduction of more than 65% from the IL-4 stimulated control. In addition, a positive trend in inflammation modulation vs. one anthocyanin (ACN) in the BC extracts was shown. This study has demonstrated the beneficial inflammation modulatory effects of polyphenolic BC extracts, which could be related to cyanidin 3-O-rutinoside content. These results may have therapeutic potential for asthma.

The allergic disease of asthma is characterized by an infiltration of inflammatory cells to the lung, a process co-ordinated by T-helper (TH) cells. The TH2 cytokine Interleukin (IL)-4 promotes infiltration of eosinophils to sites of inflammation. Eosinophil-selective chemoattractant cytokines (eg. eotaxins) are synthesized by lung epithelial cells. Eotaxin-3 is expressed at high levels in the asthmatic lung, predominantly after IL-4 stimulation. Eotaxin-3 is therefore a marker of inappropriate airway inflammation. Polyphenolic (PP) compounds found in high concentrations in berries may have beneficial effects in inflammatory conditions. Plant and Food Research produced high-PP extracts of blackcurrant (BC) cultivars that were tested for inflammation modulating effects. Since high doses of PPs have been shown to cause cell death, we tested two BC cultivars at a range of concentrations in a cell viability (WST-1) assay. While no toxic effects were attributable to the BC extracts (1-50µg/ml), a dose-related trend in cell death was observed and therefore 10µg/ml was chosen for further experiments Ten BC cultivars were compared for efficacy by measuring eotaxin-3 production in IL-4 stimulated human lung epithelial (A549) cells in vitro. Cells were incubated with BC extracts (10µg/ml) and IL-4 (10ng/ml) for 24 hours. The supernatants were then quantified for eotaxin-3 levels by an enzyme-linked immunosorbent assay (ELISA). All ten BC extracts reduced eotaxin-3 levels after stimulation with IL-4, and six BC extracts were effective by statistically significant levels (P<0.05), (BC cultivars -01, -02, -03, -05, -09 & -10). Of those, BC extracts of four cultivars demonstrated a reduction of more than 65% from the IL-4 stimulated control. In addition, a positive trend in inflammation modulation vs. one anthocyanin (ACN) in the BC extracts was shown. This study has demonstrated the beneficial inflammation modulatory effects of polyphenolic BC extracts, which could be related to cyanidin 3-O-rutinoside content. These results may have therapeutic potential for asthma.

The allergic disease of asthma is characterized by an infiltration of inflammatory cells to the lung, a process co-ordinated by T-helper (TH) cells. The TH2 cytokine Interleukin (IL)-4 promotes infiltration of eosinophils to sites of inflammation. Eosinophil-selective chemoattractant cytokines (eg. eotaxins) are synthesized by lung epithelial cells. Eotaxin-3 is expressed at high levels in the asthmatic lung, predominantly after IL-4 stimulation. Eotaxin-3 is therefore a marker of inappropriate airway inflammation. Polyphenolic (PP) compounds found in high concentrations in berries may have beneficial effects in inflammatory conditions. Plant and Food Research produced high-PP extracts of blackcurrant (BC) cultivars that were tested for inflammation modulating effects. Since high doses of PPs have been shown to cause cell death, we tested two BC cultivars at a range of concentrations in a cell viability (WST-1) assay. While no toxic effects were attributable to the BC extracts (1-50µg/ml), a dose-related trend in cell death was observed and therefore 10µg/ml was chosen for further experiments Ten BC cultivars were compared for efficacy by measuring eotaxin-3 production in IL-4 stimulated human lung epithelial (A549) cells in vitro. Cells were incubated with BC extracts (10µg/ml) and IL-4 (10ng/ml) for 24 hours. The supernatants were then quantified for eotaxin-3 levels by an enzyme-linked immunosorbent assay (ELISA). All ten BC extracts reduced eotaxin-3 levels after stimulation with IL-4, and six BC extracts were effective by statistically significant levels (P<0.05), (BC cultivars -01, -02, -03, -05, -09 & -10). Of those, BC extracts of four cultivars demonstrated a reduction of more than 65% from the IL-4 stimulated control. In addition, a positive trend in inflammation modulation vs. one anthocyanin (ACN) in the BC extracts was shown. This study has demonstrated the beneficial inflammation modulatory effects of polyphenolic BC extracts, which could be related to cyanidin 3-O-rutinoside content. These results may have therapeutic potential for asthma.