Title: Body composition in children with different movement programm Objectives: The main objektive of this work is analysis of body composition while using BIA method children of younger school age and consired differences in physical components with regard to different movement programm. Methodes: Body composition was measured by bioelectrical impedance analysis device Bodystat 1500. Data was processed in Microsoft Excel. Followed parameters are the percentage of body fat, the absolute amount of fat-free mass in kg, the proportion of total body water. The research participated in total 75 probands agend 10-11 years of average height 147,7 cm and weight 42,1 kg. The research includes regularly training 25 children from RC Mountfield Říčany (training 3 times a week + 2x physical education) with a medium to hight intensity exersice of 360 minutes per week, regularly training 25 children from Říčany Fight Club (2x weekly training + 2x physical education) with a medium to hight intensity exersice of 270 minutes per week and 25 children from the Nemo Říčany Elementary School where the physical education 90 minutes per week. The research group is made up of boys only, because girl with a higher percentage of total body fat have a higher BFM then boys. Results: The average proportion of body fat in boys...

Title: Influence of nutrition stereotyps on body composition - body weight, body fat, muscle mass and body water. Objectives: The aim of this work is to influence changes in body composition in women of various ages, body weight, occupation and movement regime. By adjusting eating habits reduce the amount of body fat while maintaining muscle mass. Methods Basic dietary recommendations for the ratio of macro-nutrients and time aspects of food intake according to the general body weight reduction recommendation. The bioimpedance method is used for assessment of body composition and selected circuits are evaluated at the same time. Based on this data, an individual diet plan was developed for each woman and the dietary effect was continuously evaluated. The study involved six women. Four overweight and obesity weighing 92 ± 17 kg and BMI 35 ± 4 kg/ . Two with a normal weight of 60 ± 2 kg and a BMI of 22.5 ± 0.5 kg/ Average age of women 40 ± 15 years. Results: After the individual diet was applied for three months, we found significant changes in women with higher body weight. They reduced body weight by 6 ± 1 kg and body fat by 5 ± 1 kg. In addition, these persons reduced the waist circumference by 5 ± 2 cm and the hips by 4 ± 2 cm. For the remaining two subjects with normal body weight, we did not...

A need for a quantitative method to determine body water level has been identified by a team of Clinical Innovation Fellows at the Centre for Technology in Medicine and Health (CTMH). A reliable way to determine body water level would bring great benefits to the healthcare sector, where no optimal method is available at the time of writing. A possible solution is a sensor that would measure alterations in skin properties due to changes in total body water. CTMH has had an idea of such a sensor, which is evaluated in this work. At an early stage of this evaluation process, it became clear that the research regarding correlations between skin properties and body hydration level was not sufficient to warrant the initiation of a sensor development process. Therefore, the main objective of this thesis became to investigate such correlations. An extensive literature review is presented, from which an experiment was developed. The experiment was performed on four human test subjects and involved measurements of skin thickness and elasticity parameters, before and after a weight loss of 3.2-3.7 % due to dehydration. The results showed clear decreases in skin thickness and indications of alterations in skin distensibility as well as in the skin’s immediate elastic response to applied negative pressure. It could also be seen that skin at different body sites does not respond in the same way - calves showed more distinct results than thighs and volar forearm. The material provided in this thesis encourages further studies of the correlation between the mentioned properties and total body water. If a predictable correlation can be found, a sensor development process could start. A reliable way to determine body water level would bring great benefits to the healthcare sector, where no optimal method is available at the time of writing. / Ett behov av att kvantitativt kunna mäta kroppens vattennivå har identifierats av Clinical Innovation Fellowship vid Centrum för Teknik i Medicin och Hälsa (CTMH). Ett tillförlitligt sätt att mäta kroppens vattennivå skulle gynna hälso- och sjukvården på många sätt då ingen optimal metod är tillgänglig i dagsläget. En möjlig lösning skulle kunna vara en sensor som mäter variationer i hudegenskaper till följd av förändringar i kroppens vattennivå. CTMH har haft en idé om en sådan sensor, vilken utvärderas i detta arbete. I ett tidigt skede av utvärderingsprocessen framkom det tydligt att tillräcklig forskning saknades gällande korrelationer mellan hudens egenskaper och kroppens vattennivå. Det huvudsakliga syftet med detta masterexamensarbete blev därför att undersöka sådana korrelationer. En omfattande litteraturgransking gjordes, och utifrån denna utformades ett experiment. Experimentet utfördes på fyra testpersoner och innefattade mätningar av hudens tjocklek samt elasticitetsparameterar. Dessa utfördes före och efter viktnedgång av 3,2-3,7 % till följd av vattenförlust. Resultaten visade på en tydlig minskning av hudtjockleken samt indikationer på förändringar av hudens tänjbarhet samt dess omedelbara elastiska respons vid pålagt negativt tryck. Det visade sig också att huden inte reagerar på samma sätt på olika kroppsdelar - vader visade tydligare förändringar jämfört med lår och armar. Det material som presenteras i detta examensarbete uppmuntrar till fortsatt utredning av korrelationer mellan de nämnda hudegenskaperna och kroppens vattennivå. Om det går att förutse korrelationer finns det förutsättningar för att påbörja utveckling av en sensor.

Zweitveröffentlichung / Die Anwendbarkeit der Verdünnungsmethode mit Hilfe des Markers Deuteriumoxid (D2O) zur in vivo-Erfassung der sich ändernden Körperzusammensetzung wurde an 53 Ziegenlämmern der Rasse Bunte Deutsche Edelziege im Gewichtsabschnitt von 4 kg bis 20 kg LM überprüft. Bei einer Lebendmasse von 4, 8, 12, 16 bzw. 20 kg wurde den Tieren nach einer 13- bis 18stündigen Nüchterung D2O injiziert und nach weiteren vier bis fünf Stunden Blutproben gezogen. Bei jeweils einem Teil der Tiere erfolgte anschließend die Schlachtung und die chemische Analyse der Körper zur Bestimmung des Ganzkörperwassergehaltes, der Chymusmasse und der Leerkörperzusammensetzung. Die Messungen der D2O-Konzentrationen in den Blutproben und die chemische Analyse der Ganzkörper ergaben eine Überschätzung des Ganzkörperwassergehaltes durch die Verdünnungsmethode um im Mittel 3,99 %. Zwischen dem chemisch bestimmten und dem geschätzten Wert besteht eine enge lineare Beziehung. Die Schätzung des Inhaltes des Gastro-Intestinaltraktes (Chymus) dient zur Schätzung der Leerkörpermasse. Dessen Variabilität lässt sich im Wesentlichen durch die Lebendmasse erklären. Die chemische Analyse der Tierkörper zeigte, dass die Zusammensetzung des fettfreien Leerkörpers im hohen Maß von der Leerkörpermasse abhängt. Anhand von allometrischen Regressionsgleichungen wurden die Bestandteile der fettfreien Leerkörpermasse geschätzt und daraus die Zusammensetzung des fetthaltigen Leerkörpers ermittelt. Der Vergleich der mittels chemischer Analyse bestimmten bzw. der Verdünnungsmethode geschätzten Leerkörperzusammensetzung ergab eine hohe Übereinstimmung beim Wasser-, Rohprotein- und Rohaschegehalt. Der Rohfettgehalt wies dagegen größere Abweichungen auf. Als Ursache wurde die Streuung der geschätzten Chymusmasse bzw. Chymuswassermasse aufgedeckt. Das Fazit ist, dass sich die D2O-Verdünnungsmethode zur in vivo-Bestimmung der sich ändernden Körperzusammensetzung von Ziegenlämmern im Gewichtsabschnitt von 4 kg bis 20 kg LM eignet. / On young goats (breed Bunte Deutsche Edelziege) with a live weight range of 4 kg up to 20 kg the suitability of the dilution method using the marker deuterated water (D2O) was checked in order to measure the changes of body composition during growth on living animals (in vivo). At a live weight of 4, 8, 12, 16 and 20 kg the animals received an intrave¬nous dose of D2O after a fastening time of 13 to 18 hours. Blood samples were taken after a waiting period of 4 to 5 hours. In connection with the last blood sample in each period a part of the animals were slaughtered to determine the total body water content, the digesta mass and the empty body composition by chemical analysis. The determination of the marker concentration in the blood samples and the chemical analysis of the total bodies showed that the dilution method overestimated total body water content by 3,99 %. There is a close linear relationship between the estimated and the chemically determined content of total body water. The estimation of the digesta mass serves for the estimation of the empty body mass. Its variation mainly depends on the live weight. The chemical analysis of the animal bodies revealed that the composition of the fat-free empty body is mainly dependent on the empty body mass. Using allometric equations the components of the fat-free empty body mass were estimated and thus the composition of the empty body could be calculated. The comparison of the chemically determined components with the estimated components of the empty body showed a high agreement concerning the content of water, protein and ash. However estimation of the fat content is less accurate. The reason is the variation of the estimated digesta mass and the digesta water mass. It can be concluded that the dilution technique using deuterated water is a practicable method to determine in vivo the changing body composition of growing kids with a live weight range of 4 kg up to 20 kg.

Title: Influence of nutrition stereotypes on body fat reduction Objectives: The aim of this work is to achieve a reduction in body fat through appropriate eating habits. Acquired theoretical knowledge applied to practice for six women of different age and movement regime. Inform about the procedures of this intervention. Verify the validity of theoretical knowledge in practice. Achieve the desired results. Methods Basic dietary recommendations, peer-to-peer comparisons and time aspects of food intake. Furthermore, bioimpedance measurements on the In Body 230 instrument and physical body measurements by a tape method to determine the baseline status of the examined women and to subsequently monitor their running state at specified intervals. Developing a diet for each of these women and evaluating the on-going status. Results: The result of this diploma thesis is to make changes in eating habits that will lead to the reduction of body fat. Partial results are the protocols from the continuous measurement on the In Body 230 at the Lunacor s.r.o. In Kroměříž and fitness center Fit2b s.r.o. In Prague 8 - Bohnice.. Key words: Nutrition, way of living, lifestyle, obesity, BMI, dietary, basal metabolism, nutrition ratio, nutritional habits, reduction, body weight, body fat, muscle mass, body water,...

Volume overload is a common feature in patients on haemodialysis (HD). This contributes significantly to the cardiovascular disease burden seen in these patients. Clinical assessments of the volume state are often inaccurate. Techniques such as interdialytic blood pressure, relative blood volume monitoring, bioimpedance are available to improve clinical effectives. However all these techniques exhibit significant shortcomings in their accuracy, reliability and applicability at the bed side. We evaluated the usefulness of a dual compartment monitoring technique using Continuous Segmental Bioimpedance Spectroscopy (CSBIS) and Relative Blood Volume (RBV) as a tool to assess hydration status and determine dry weight. We also sought to evaluate the role of Atrial Natriuretic Peptide (ANP) and B-type Natriuretic Peptide (BNP) as a volume marker in dialysis patients. The Retrospective analysis of a historical cohort (n = 376, 55 Diabetic) showed a significant reduction in post-dialysis weights in the first three months of dialysis (72.5 to 70kg, p<0.027) with a non-significant increase in weight between months 6-12. The use of anti-hypertensive agents reduced insignificantly in the first 3 months, increased marginally between months 3-6 and significantly increased over the subsequent 6 months. The residual urea clearance (KRU) fell and dialysis times increased. The cohort was very different to that dialysing at Tassin and showed a dissociation between weight reduction and BP control. This may relate to occult volume overload. CSBIS-RBV monitoring in 9 patients with pulse ultrafiltration (pulse UF) showed distinct reproducible patterns relating to extra cellular fluid (ECF) and RBV rebound. An empirical Refill Ratio was then used to define the patterns of change and this was related to the state of their hydration. A value closer to unity was consistent with the attainment of best achievable target weight. The refill ratio fell significantly between the first (earlier) and third (last) rebound phase (1.97 ± 0.92 vs 1.32 ± 0.2). CSBIS monitoring was then carried out in 31 subjects, whilst varying dialysate composition, temperature and patient posture to analyse the effects of these changes on the ECF trace and to ascertain whether any of these interventions can trigger a change in the slope of the ECF trace distinct to that caused by UF. Only, isovolemic HD caused a change in both RBV and ECF in some patients that was explained by volume re-distribution due to gravitational shifts, poor vascular reactivity, sodium gradient between plasma and dialysate and the use of vasodilating antihypertensive agents. This has not been described previously. These will need to be explored further. The study did demonstrate a significant lack of comparability of absolute values of RECF between dialysis sessions even in the same patient. This too has not been described previously. This is likely to be due to subtle changes in fluid distribution between compartments. Therefore a relative changes must be studied. This sensitivity to subtle changes may increase the usefulness of the technique for ECF tracking through dialysis. The potential of dual compartment monitoring to track volume changes in real time was further explored in 29 patients of whom 21 achieved weight reductions and were able to be restudied. The Refill Ratio decreased significantly in the 21 patients who had their dry weights reduced by 0.95 ± 1.13 kg (1.41 ± 0.25 vs 1.25 ± 0.31). Blood pressure changes did not reach statistical significance. The technique was then used to examine differences in vascular refill between a 36oC and isothermic dialysis session in 20 stable prevalent patients. Pulse UF was carried out in both these sessions. There were no significant differences in Refill Ratios, energy removed and blood pressure response between the two sessions. The core temperature (CT) of these patients was close to 36oC and administering isothermic HD did not confer any additional benefit. Mean BNP levels in 12 patients during isovolemic HD and HD with UF did not relate to volume changes. ANP concentrations fell during a dialysis session in 11 patients from a mean 249 ± 143 pg/ml (mean ± SD) at the start of dialysis to 77 ± 65 pg/ml at the end of the session (p<0.001). During isolated UF levels did not change but fell in the ensuing sham phase indicating a time lag between volume loss and decreased generation. (136±99 pg/ml to 101±77.2 pg/ml; p<0.02) In a subsequent study ANP concentrations were measured throughout dialysis and in the post-HD period for 2 hours. A rebound in ANP concentration was observed occurring at around 90 min post-HD. The degree of this rebound may reflect the prevailing fluid state and merit further study. We have shown the utility of dual compartment monitoring with CSBIS-RBV technique and its potential in assessing volume changes in real time in haemodialysis patients. We have also shown the potential of ANP as an independent marker of volume status in the same setting. Both these techniques merit further study.