Focusing on four novels by Janet Frame in dialogue with texts by Freud, Zizek, Lacan, and Silverman, my project theorizes trauma as the basis for both an ethical and an interpretive practice. Frame's fiction develops a cultural psychology, showing how the factors of narcissistic fantasy and the incapacity to mourn contribute to physical and epistemic aggression committed along divides of ethnicity, gender, and linguistic mode of expression. Employing trauma as a figure for an absolute limit to what can be remembered or known, I suggest that reconciliation with whatever is inaccessible, lacking, or dead within an individual or collective self fosters a non-violent relation with others. I begin by querying the place of "catharsis" within hermeneutic literary interpretation, focusing on the construction of Frame within the New Zealand literary industry. With Erlene's adamantine silence at its centre, Scented Gardens for the Blind (1964) rejects the hermeneutic endeavour, exemplified by Patrick Evans' critical work on Frame, to make a text "speak" its secrets. My readings of Intensive Care (1910) and The Adaptable Man (1965) address inter-generational repetitions of violence as the consequences of the failure to recognise and work through the devastations of war. The masculine fantasy of totality driving the Human Delineation project in Intensive Care has a linguistic corollary in Colin Monk's pursuit of the Platonic ideality of algebra, set against Milly's "degraded" punning writing. In The Adaptable Man, the arrival of electricity ushers in a new perceptual regime that would obliterate any "shadow" of dialectical negativity or internal difference. The thesis ends with a swing toward conciliation and emotional growth. The homosexual relationship depicted in Daughter Buffalo (1972) offers a model of transference, defined as a transitional, productive form of repetition that opens Talbot to his ethnic and familial inheritance. Working from within a radical form of narcissism, the novel reformulates masculinity by embracing loss as "phallic divestiture" (Kaja Silverman).

Made available in DSpace on 2014-06-11T19:23:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-12-06Bitstream added on 2014-06-13T19:50:28Z : No. of bitstreams: 1 marchiori_jmg_me_arafcf.pdf: 295739 bytes, checksum: 2762d0dcb458c6dea6e3dcb616a93875 (MD5) / Universidade Estadual Paulista (UNESP) / A produção animal sob sistema orgânico certificado ainda é pouco explorada no país, mas já existem criações de búfalas e de vacas leiteiras neste sistema, sendo o queijo mussarela o principal produto obtido a partir do leite bubalino. A oferta do produto orgânico com qualidade devidamente avaliada possibilita um maior interesse para o seu consumo. Avaliou-se a qualidade nutricional do queijo mussarela elaborado com leite de búfala e de vaca produzido em sistemas convencional e orgânico. Entre os queijos, as mussarelas de leite de búfala apresentaram maior quantidade em proteína. Concluiu-se que os queijos mussarela elaborados com leite de búfala e de vaca apresentaram elevado valor nutricional. Em relação aos queijos elaborados com leite de búfala os obtidos na produção orgânica apresentaram qualidade da proteína superior aos obtidos no sistema convencional, avaliada pela digestibilidade, valor biológico e utilização líquida da proteína. O valor biológico do queijo orgânico de leite de vaca foi superior ao convencional. Entre os queijos avaliados os elaborados com leite de búfala apresentaram menores influências nos níveis de triglicerídeos sanguíneos. / The certified organic animal production it is not well explored in the country, but there are buffalo and dairy cows raised in this type of system. Within the buffalo population, mozzarella cheese is the main product obtained, and the existence of certified organic product will permit a greater consumption. Therefore, the nutritional quality of conventional and organic buffalo mozzarella cheese was evaluated. For that, forty recently weaned male Wistar rats equally distributed in five groups of eight rats were used in the study. Between the cheeses from the two species, the buffalo mozzarella presented higher protein than the cow mozzarella. In conclusion, the mozzarella cheese from buffalo or cow presented an elevated nutritional value. In relation to the cheese made from buffalo, those obtained from the organic system presented superior protein quality to those obtained from the conventional system, as indicated by the digestibility, biological value and protein liquid utilization. The cow organic cheese biological value was superior to the conventional cheese. Among the cheeses evaluated, the ones from buffalo had the lowest influence in the blood triglycerides levels.

The research project, conceptualized through a comparative historical framework, focuses on an analysis of American and English education policy from 1964 to 2000 with particular emphasis on the inter-relationships between education policy and socio-economic disadvantage. Although the focus of the project is primarily the last four decades of the twentieth century, there is an initial consideration of immediate post-war discourses on poverty and education focusing on the impact that these had upon educational structures and curricula. Critical theory, particularly as conceptualized by Jürgen Habermas, and the Culture of Poverty thesis advanced by Oscar Lewis, form the methodological frameworks that underpin the research project. The research, which was conducted in two post-industrial communities with significant rates of socio-economic deprivation and records of poor educational attainment within secondary education, considers the impact of national policy upon the communities, particularly in relationship to socio-economic deprivation, access to education, equality of opportunity and equality of outcomes. The research design utilises the case study method to scrutinise two secondary schools within these communities as a means of analysing how teachers negotiated the implementation of education policies for their respective student populations. Documentary evidence and oral histories provide the methods to delve into this interconnection between education and socio-economic deprivation while modified Skinnerian and Eastonian frameworks provide the foundations upon which to analyse the data. The dissertation is not meant to trace the history of two schools and two communities but to see the schools and communities as a microcosm of American and English secondary education. The intention, therefore, is to employ the research findings to prescribe potential and future policy directions. Essentially, tracing educational history to understand it while utilising educational history as a tool to inform new and innovative policy where education can ameliorate socio-economic deprivation in each nation.

Made available in DSpace on 2014-06-11T19:26:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-02-29Bitstream added on 2014-06-13T18:53:57Z : No. of bitstreams: 1 venancio_lpr_me_sjrp.pdf: 1068047 bytes, checksum: d85b41abf589322ab5174b5d9c2b731e (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Nsf - Nacional Science Foundation / O Brasil é o país com o maior rebanho de búfalo de rio, Bubalus bubalis, no continente americano e também o maior produtor deste animal fora do continente asiático. A produção de leite e derivados vem aumentando devido à potencialidade dessa espécie em produzir leite com baixos custos e elevado rendimento industrial. Apesar das características economicamente importantes inerentes ao búfalo, as pesquisas científicas são limitadas em muitos países onde o búfalo é economicamente importante e como conseqüência a pesquisa genômica encontrase defasada quando comparado com outras espécies de interesse econômico. Marcadores moleculares são essenciais para avaliar informações qualitativas e quantitativas da diversidade molecular com o objetivo de aperfeiçoar a utilização e conservação da variabilidade genética e o relacionamento entre vários rebanhos. Microssatélites diferem dos outros tipos de seqüências de DNA por seu extenso polimorfismo dentro e entre populações, sendo considerados excelentes tipos de marcadores moleculares. O presente estudo teve como objetivos construir bibliotecas genômicas parciais enriquecidas com microssatélites, isolar e caracterizar os microssatélites obtidos, além de determinar as condições de amplificação por PCR para alguns dos locos isolados da biblioteca. Foram desenvolvidas 6 bibliotecas genômicas parciais – (CA)15 , (CT)15 , (AGG)8 , (GATA)8 , (GAAA)8, (AAAAC)8. O processo de clonagem gerou um total de 1.824 clones recombinantes, sendo que 954 foram seqüenciados para identificação de microssatélites. Cento e treze microssatélites foram encontrados. Desses, foram identificados 96 com unidade de repetição dinucleotídica (84,95%), 10 repetições trinucleotídicas (8,85%), 6 repetições tetranucleotídicas (5,3%) e 1 repetição pentanucleotídica (0,89%). As seqüências de microssatélites... / Brazil is the largest river buffalo (Bubalus bubalis) breeding center outside the Asian continent, the origin of the domestic buffalo. All buffalo breeds have a strong milk/meat attributes. Their extensive use in agriculture world wide, and especially in developing countries, begs for genetic resources to evaluate and improve traits important to local and regional economies. Among the different types of DNA markers, microsatellites are useful for studying genetic variability within and between populations due its high heterozygosity. The goal of this study was to construct partial genomic libraries enriched with repeated sequences to isolate and characterize microsatellites for river buffalo. The cloning process generated a total of 1824 recombinant clones, from which 954 were sequenced for the microsatellites search. One hundred and thirteen new microsatellites were isolated, containing the following type of repeats: dinucleotide repeats (96 sequences - 84.95%), trinucleotide repeats (10 sequences - 8.85%), tetranucleotide repeats (6 sequences – 5.3%) and pentanucleotide repeats (1 sequence - 0.89%). The new microsatellites were structurally categorized into 3 categories: pure repeats (90 sequences - 79.64%), pure interrupted (21 sequences - 18.59%) and compound interrupted repeats (2 sequence – 1.77%). PCR primer pairs were designed for ten microsatellites, from which four had the PCR conditions optimized. The microsatellites isolated in this study will be used to evaluate the genetic variability of Brazilian populations of river buffalo and to the gene mapping program established for this specie.

Made available in DSpace on 2014-06-11T19:32:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-02-29Bitstream added on 2014-06-13T20:23:17Z : No. of bitstreams: 1 miziara_mn_dr_sjrp.pdf: 8388230 bytes, checksum: 735d0547b6a7d4ff2141893c4f8644a7 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Nsf - Nacional Science Foundation / O cromossomo 1 do genoma bubalino (BBU1), o maior cromossomo do cariótipo do búfalo de rio, é um cromossomo submetacêntrico que possui homologia com os cromossomos 1 e 27 do genoma bovino. Neste trabalho, apresentamos o primeiro mapa RH para este cromossomo, construído por meio da utilização de um painel de células somáticas híbridas irradiadas búfalo-roedor, denominado BBURH5000. O mapa consistiu em 69 marcadores derivados dos cromossomos bovinos BTA1 e BTA27, incluindo 48 genes codificantes, 17 microssatélites e quatro ESTs distribuídos em dois grupos de ligação. A freqüência de retenção observada entre os marcadores variou de 17.8% a 52.2%. A ordem dos marcadores dentro dos grupos de ligação foi, em sua maioria, idêntica a ordem encontrada nos mapas RH e de seqüência do genoma bovino. A análise comparativa do mapa RH obtido para BBU1 com o genoma humano revelou oito blocos homólogos de sintenia entre BBU1 e segmentos correspondentes dos cromossomos humanos 3, 4, 8 e 21, sendo a maioria deles rearranjados quanto à ordem dos genes e orientação dos blocos. Os blocos de sintenia também foram comparados com os genomas de outras espécies de mamíferos, como boi, chimpanzé, cachorro e cavalo. Considerando a inexistência de mapas de ligação para o búfalo de rio, o mapa RH obtido neste estudo fornece dados essenciais para os estudos comparativos deste cromossomo com qualquer outra espécie de mamífero. / The largest chromosome in the river buffalo karyotype, BBU1, is a submetacentric chromosome with reported homology between BBU1q and bovine chromosome 1 and between BBU1p and BTA27. We present the first radiation hybrid map of this chromosome containing 69 cattle derived markers including 48 coding genes, 17 microsatellites and four ESTs distributed in two linkage groups. The RH map was constructed based on the analysis of a recently developed river buffalo-hamster whole genome radiation hybrid panel (BBURH5000). The retention frequency of individual markers across the panel ranged from 17.8% to 52.2%. With few exceptions, the order of markers within linkage groups is identical to the order established for corresponding cattle sequence and RH maps. Comparative analysis between BBU1-RH5000 and the human genome revealed eight homologous synteny blocks corresponding to HSA3q, HSA4q, HSA8p and HSA21q. Most of the blocks showed rearrangements in the gene order and in the orientation of the synteny blocks. The synteny blocks were also compared with other mammalian genomes, such as bovine, chimpanzee, dog and horse. Considering that a genetic linkage map does not exist for river buffalo, the radiation hybrid map generated in this study provides valuable data for comparative mapping of BBU1 chromosome.

Movements of animals presumably reflect their changing needs and the changing availability of necessary resources. In cold climates, snakes often make long seasonal migrations between hibernacula and summer habitats, Finding suitably deep hibernacula with minimal delay could be critical. I hypothesized that such animals should have highly developed navigational ability. By contrast, snakes living in mild climates can hibernate in shallow sites and probably do not migrate; if so, they might be expected to show poorly developed orientation mechanisms!. The objectives of this study were to determine movement patterns and navigational ability of garter snakes (Thamnophis) living in a mild climate and compare them with a congeneric population known to be migratory. From 1986 - 1988 I examined, using mark-recapture, movement behaviour of two populations of garter snakes at Spectacle Lake Provincial Park (SLPP) on Vancouver Island, British Columbia, focusing on several components typically associated with migratory behaviour - distances travelled, population directionality, fidelity to seasonal sites, distinctness of seasonal habitats, and route directness. Thamnophis sirtalis, the common garter snake, is the most widely distributed North American snake species and high latitude populations are migratory. Thamnophis ordinoides, the northwestern garter snake, is restricted to the Pacific northwest and migratory behaviour has never been reported. Both species displayed combinations of traits clearly suggesting nonmigratory behaviour. These included short-distance (< 500 m), random movements, a lack of den fidelity, and variation in the maintenance of specific home ranges between successive years. Home ranges overlapped between individuals, averaged less than 0.3 ha measured over a single active season, and were not clearly distinct from denning areas. Although some directionality of movement was evident, it was likely related to foraging strategy and unlike the typical unidirectional movements undertaken by migrating snakes, Sexual and reproductive differences in any of these traits generally were not significant. The navigational abilities of a migratory population of T. sirtalis from Wood Buffalo National Park (WBNP) in northern Alberta were examined as were those of the nonmigratory populations of snakes from SLPP. Displacement studies were carried out during the active seasons of 1986 - 1988 to determine the level of orientational abilities present in each population and to examine potential orientation cues. Snakes were displaced from their home range and tested in an arena under a variety of conditions, The results demonstrated that T. sirtalis from both SLPP and WBNP possessed advanced navigational abilities. Advanced skills may be absent in T. ordinoides. Thamnophis sirtalis at both study sites demonstrated time-compensated solar orientation as determined by 6 hr phase-delayed tests. Pheromone trails produced by recently copulated females (but not unmated females) also provided an orientation guide for displaced WBNP males, but results from SLPP were less conclusive. Thamnophis ordinoides did not respond in a discernible way to either cue. Navigational skills thus vary relatively little between migrating and nonmigrating populations of the same species but may be poorly developed in completely nonmigratory species. / Graduate

Made available in DSpace on 2014-06-11T19:29:15Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-02-25Bitstream added on 2014-06-13T20:19:15Z : No. of bitstreams: 1 santana_am_me_jabo.pdf: 1036969 bytes, checksum: 219ad2d9cc666bb8a7c481a54c0a4cf9 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O búfalo de rio (Bubalus bubalis) é uma espécie de interesse econômico pertencente à família Bovidae, utilizado para a produção de carne e leite, além de força de trabalho no campo, constituindo-se em um animal de tripla aptidão. Recentemente, a construção de um painel de células somáticas híbridas irradiadas (linhagens celulares) contendo fragmentos do genoma do búfalo de rio, incorporado ao genoma de hamster (BBURH5000), está permitindo, pela primeira vez, o mapeamento de todos os cromossomos bubalinos. A utilização desse painel de células associado a análises estatísticas, está gerando mapas genômicos de alta resolução contendo diferentes tipos de marcadores moleculares. O presente trabalho teve por objetivo utilizar este painel de células para gerar grupos de ligação com os marcadores mapeados no cromossomo 9 bubalino e assim comparar a organização dos grupos de ligação obtidos com aqueles já descritos para o cromossomo 7 bovino. Para tal, foram testados com DNA bubalino, seqüências de “primers” para PCR de 26 marcadores moleculares previamente mapeados no cromossomo 7 bovino, o qual foi descrito na literatura como o homólogo ao cromossomo 9 de búfalo. Do total de marcadores testados, 18 geraram produtos de PCR adequados ao mapeamento utilizando o painel BBURH5000, sendo que destes 18, foi possível a genotipagem final de 10 marcadores. A partir das análises de ligação com os 10 marcadores genotipados, foi possível distribuir os mesmos em três grupos de ligação no BBU9. A análise comparativa entre os grupos de ligação do BBU9 e do BTA7 revelou a presença dos mesmos grupos de ligação nos cromossomos de ambas espécies, evidenciando conservação de sintenia. / The river buffalo (Bubalus bubalis), a member of the Bovidae family, is an economically important livestock specie useful to produce milk and meat, as well as source of labor, comprising an animal of triple aptness. The recent construction of a whole-genome 5000-rad radiation hybrid somatic cell panel for the river buffalo genome (BBURH5000), is allowing for the first time the mapping of all chromosomes from the specie. The use of BBURH5000 panel associated with statistical analyses is generating high-resolution RH maps integrating different types of molecular markers. The goal of this study was to genarate linkage groups with the markers mapped on the river buffalo chromosome 9 (BBU9), using the BBURH5000 panel, and then compare these groups with those already described in BTA7. Previous studies have identified bovine chromosome 7 as homologous to BBU9. Cattle derived PCR primers from 26 markers, previously mapped in cattle, were tested with buffalo DNA. A total of 18 of the 26 markers amplified PCR products suitable for RH mapping. From these 18 markers, it was possible the genotyping of 10. The analyses of linkage with the 10 markers genotyped turned possible to distribute them in three linkage groups on the BBU9. The comparative analyses between the linkage groups of BBU9 and BTA7 revealed the presence of the same linkage groups on the chromosome of both species, revealing conservation of synteny between them.

Orientador: Maria Regina Barbieri de Carvalho / Banca: Marta Regina Verruma Bernardi / Banca: Humberto Tonhati / Resumo: A produção animal sob sistema orgânico certificado ainda é pouco explorada no país, mas já existem criações de búfalas e de vacas leiteiras neste sistema, sendo o queijo mussarela o principal produto obtido a partir do leite bubalino. A oferta do produto orgânico com qualidade devidamente avaliada possibilita um maior interesse para o seu consumo. Avaliou-se a qualidade nutricional do queijo mussarela elaborado com leite de búfala e de vaca produzido em sistemas convencional e orgânico. Entre os queijos, as mussarelas de leite de búfala apresentaram maior quantidade em proteína. Concluiu-se que os queijos mussarela elaborados com leite de búfala e de vaca apresentaram elevado valor nutricional. Em relação aos queijos elaborados com leite de búfala os obtidos na produção orgânica apresentaram qualidade da proteína superior aos obtidos no sistema convencional, avaliada pela digestibilidade, valor biológico e utilização líquida da proteína. O valor biológico do queijo orgânico de leite de vaca foi superior ao convencional. Entre os queijos avaliados os elaborados com leite de búfala apresentaram menores influências nos níveis de triglicerídeos sanguíneos. / Abstract: The certified organic animal production it is not well explored in the country, but there are buffalo and dairy cows raised in this type of system. Within the buffalo population, mozzarella cheese is the main product obtained, and the existence of certified organic product will permit a greater consumption. Therefore, the nutritional quality of conventional and organic buffalo mozzarella cheese was evaluated. For that, forty recently weaned male Wistar rats equally distributed in five groups of eight rats were used in the study. Between the cheeses from the two species, the buffalo mozzarella presented higher protein than the cow mozzarella. In conclusion, the mozzarella cheese from buffalo or cow presented an elevated nutritional value. In relation to the cheese made from buffalo, those obtained from the organic system presented superior protein quality to those obtained from the conventional system, as indicated by the digestibility, biological value and protein liquid utilization. The cow organic cheese biological value was superior to the conventional cheese. Among the cheeses evaluated, the ones from buffalo had the lowest influence in the blood triglycerides levels. / Mestre

Resumo: O búfalo de rio (Bubalus bubalis) é uma espécie de interesse econômico pertencente à família Bovidae, utilizado para a produção de carne e leite, além de força de trabalho no campo, constituindo-se em um animal de tripla aptidão. Recentemente, a construção de um painel de células somáticas híbridas irradiadas (linhagens celulares) contendo fragmentos do genoma do búfalo de rio, incorporado ao genoma de hamster (BBURH5000), está permitindo, pela primeira vez, o mapeamento de todos os cromossomos bubalinos. A utilização desse painel de células associado a análises estatísticas, está gerando mapas genômicos de alta resolução contendo diferentes tipos de marcadores moleculares. O presente trabalho teve por objetivo utilizar este painel de células para gerar grupos de ligação com os marcadores mapeados no cromossomo 9 bubalino e assim comparar a organização dos grupos de ligação obtidos com aqueles já descritos para o cromossomo 7 bovino. Para tal, foram testados com DNA bubalino, seqüências de "primers" para PCR de 26 marcadores moleculares previamente mapeados no cromossomo 7 bovino, o qual foi descrito na literatura como o homólogo ao cromossomo 9 de búfalo. Do total de marcadores testados, 18 geraram produtos de PCR adequados ao mapeamento utilizando o painel BBURH5000, sendo que destes 18, foi possível a genotipagem final de 10 marcadores. A partir das análises de ligação com os 10 marcadores genotipados, foi possível distribuir os mesmos em três grupos de ligação no BBU9. A análise comparativa entre os grupos de ligação do BBU9 e do BTA7 revelou a presença dos mesmos grupos de ligação nos cromossomos de ambas espécies, evidenciando conservação de sintenia. / Abstract: The river buffalo (Bubalus bubalis), a member of the Bovidae family, is an economically important livestock specie useful to produce milk and meat, as well as source of labor, comprising an animal of triple aptness. The recent construction of a whole-genome 5000-rad radiation hybrid somatic cell panel for the river buffalo genome (BBURH5000), is allowing for the first time the mapping of all chromosomes from the specie. The use of BBURH5000 panel associated with statistical analyses is generating high-resolution RH maps integrating different types of molecular markers. The goal of this study was to genarate linkage groups with the markers mapped on the river buffalo chromosome 9 (BBU9), using the BBURH5000 panel, and then compare these groups with those already described in BTA7. Previous studies have identified bovine chromosome 7 as homologous to BBU9. Cattle derived PCR primers from 26 markers, previously mapped in cattle, were tested with buffalo DNA. A total of 18 of the 26 markers amplified PCR products suitable for RH mapping. From these 18 markers, it was possible the genotyping of 10. The analyses of linkage with the 10 markers genotyped turned possible to distribute them in three linkage groups on the BBU9. The comparative analyses between the linkage groups of BBU9 and BTA7 revealed the presence of the same linkage groups on the chromosome of both species, revealing conservation of synteny between them. / Orientadora: Maria Elisabete Jorge Amaral / Coorientadora: Vera Fernanda Martins H. de Lima / Banca: Humberto Tonhati / Banca: Simone Cristina Méo Niciura / Mestre

Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-07-14T18:28:11Z No. of bitstreams: 1 eduardolagebisaggio.pdf: 9894299 bytes, checksum: 39e251314b1dd98e469868c1cf6f9696 (MD5) / Approved for entry into archive by Diamantino Mayra (mayra.diamantino@ufjf.edu.br) on 2016-07-19T15:47:18Z (GMT) No. of bitstreams: 1 eduardolagebisaggio.pdf: 9894299 bytes, checksum: 39e251314b1dd98e469868c1cf6f9696 (MD5) / Made available in DSpace on 2016-07-19T15:47:18Z (GMT). No. of bitstreams: 1 eduardolagebisaggio.pdf: 9894299 bytes, checksum: 39e251314b1dd98e469868c1cf6f9696 (MD5) Previous issue date: 2011-02-18 / Populações bubalinas ferais (Bubalus bubalis) são conhecidas por provocarem significativos impactos ambientais em diversas regiões do mundo, inclusive no Brasil. Uma dessas populações se encontra na Reserva Biológica do Guaporé, situada no Vale do Guaporé, Rondônia. A chegada dos búfalos no Vale ocorreu por volta da década de 1950, quando 66 animais foram trazidos para uma fazenda vizinha à Reserva. Com o abandono da fazenda, os búfalos permaneceram sem controle, aumentaram em número (atualmente estimado aprox. 4.000 cabeças) e provocaram significativas alterações ambientais na região da Reserva. Poucas medidas trataram a questão e quase nada se conhece sobre os búfalos da região. Com isso, o presente trabalho busca mapear a área de ocorrência dos animais na Reserva e fornecer propostas para o manejo da espécie. Além disso, indica como os prováveis impactos ambientais dos búfalos sobre a Reserva podem ser monitorados e avaliados. Para o mapeamento da área, três sobrevôos foram realizados. Os dados obtidos em GPS foram transferidos para softwares, os quais forneceram uma área ocupada total de 49.311 há (aprox. 8% da Reserva). Ela é composta predominantemente por campos naturais (62,5%), seguidos de 25,1% de florestas e ecótonos e 12,4% de buritizais. Da área total, 18.706 ha estão densamente ocupados por búfalos. A presença de cursos d’água perenes e grandes extensões de florestas, bem como aspectos inerentes à espécie, contribuem para a delimitação dos búfalos em uma única área. A soma da área ocupada na Reserva com a área da Fazenda Pau D’Óleo (local de introdução inicial) resulta em 60.300 ha ocupados. Existem diferentes indícios de alterações ambientais na área ocupada. Os búfalos são a principal ameaça aos ecossistemas da Reserva. Existe alto potencial de dispersão dos animais para terras vizinhas à área ocupada. Para solucionar a questão, duas propostas de erradicação foram apresentadas. A primeira constitui no abate massivo de animais e a segunda em campanhas sucessivas de esterilização. Aspectos éticos, econômicos e logísticos são avaliados. Entretanto, tais medidas de controle somente podem ser adotadas caso existam as necessárias condições de execução. Independentemente da decisão tomada, o monitoramento da população bubalina é indispensável. / Impacts of feral water buffalo populations (Bubalus bubalis) have happened worldwide included Brazil. In this country the protected area of REBIO do Guaporé in the state of Rondônia (Amazon) has been affected by water buffalo. The buffalo’s introduction in this area occurred around the 1950s, when 66 animals were brought to a farm next to REBIO do Guaporé. When the farm was abandoned the Buffaloes remained unchecked, increased in number (currently estimated approx. 4.000 animals) and resulted in significant alterations in the region of the Reserve. Few steps dealt with this issue and almost nothing is known about the buffaloes of the region. This dissertation attempted mapping the area where the animals have been, as also providing proposals for the control of the population. In addition, it indicates how the likely environmental impacts of the buffaloes on the Reserve can be monitored and evaluated. For mapping of the area, three flights were conducted. The captured data from the GPS were transferred to softwares which provided a total area occupied of 49.311 ha (nearly 8% of Reserve area). From this total Area, 18.706 ha are heavily populated by buffaloes. The area used is composed predominantly of grasslands (62.5%), followed by 25.1% of forests and ecotones and 12.4% of palm forests. The presence of perennial water courses and large extensions forests, as well as aspects related to the species, contribute to the delimitation of the buffalo in one single area. The total of the occupied area in the Reserve plus the area of Pau D'Óleo farm (place of the initial introduction of the buffaloes) results a total of 60,300 ha occupied. There are different indications of environmental changes in the occupied area. Buffaloes are the main threat to the ecosystems of the Reserve. There is high dispersion potential of animals to bordering land of the occupied area. To solve that problem, two proposals are presented for the eradication of the Buffaloes. The first is the massive slaughter of animals. The second is successive rounds of sterilization. Ethical, economic and logistical aspects were evaluated. However, control measures can be adopted only if there are necessary conditions for implementation. Regardless of the decision, the buffaloes population monitoring is essential.