published_or_final_version / abstract / Chinese / Master / Master of Philosophy

The discovery of Ni-, Cu-, and Zn-bearing Fe-sulfides from comet 81P/Wild 2 represents the strongest evidence, in the Stardust collection, of grains that formed in an aqueous environment. Crystalline sulfide assemblages from Wild 2 and the hydrothermally altered CI chondrite Orgueil were investigated. Structural and compositional characterizations of the sulfide grains from both collections reveal similarities. The Stardust samples include a cubanite (CuFe₂S₃) grain, a pyrrhotite [(Fe,Ni)₁₋ₓS]/pentlandite [(Fe,Ni)₉S₈] assemblage, and a pyrrhotite/sphalerite [(Fe,Zn)S] assemblage. Similarly, the CI-chondrite sulfides include individual cubanite and pyrrhotite grains, cubanite/pyrrhotite assemblages, pyrrhotite/pentlandite assemblages, as well as possible sphalerite inclusions within pyrrhotite grains. The cubanite is the low temperature orthorhombic form, which constrains temperature to a maximum of 210°C. The Stardust and Orgueil pyrrhotites are the 4C monoclinic polytype, which is not stable above ~250°C. The combinations of cubanite and pyrrhotite, as well as pyrrhotite and pentlandite, signify even lower temperatures. The crystal structures, compositions, and petrographic relationships of these sulfides constrain formation and alteration conditions. Taken together, these constraints attest to low-temperature hydrothermal processing. The hydrothermal conditions under which cubanite forms were investigated through thermodynamic modeling and experimental synthesis. A thermodynamic model for cubanite was developed to constrain its formation environment. Cubanite was synthesized under hydrothermal conditions consistent with those predicted for the CI-chondrite parent body. The similarity between Stardust and CI-chondrite sulfides suggest similar fluid conditions may have existed on the comet at some point in its history. The presence of cubanite in the Stardust collection has implications for large scale issues such as: heat sources in the comet-forming region; aqueous activity on cometary bodies; and the extent and mechanisms of radial mixing of material in the early nebula. The Wild 2 sulfides are most likely the products of low-temperature aqueous alteration and provide evidence of radial mixing of material from the inner solar system to the comet-forming region and possible secondary aqueous processing on the cometary body.

Under VT 2019 ägde projektet rum varav denna rapport är ett av resultaten. Projektets mål var att skapa en CI/CD pipeline vars syfte var tänkt att frekvent kunna leverera färdigtestad kod till olika molntjänster som Google Cloud Platform, Amazon Web Services och Azure. Projektspecifikationerna gavs av företaget Skira för att skapa en snabbare integrationsprocess för nya utvecklare. Detta så en ny utvecklare skulle kunna lägga mer tid på att koda istället för att gräva ner sig i leverans-/testningsprocessen. Slutprodukten ger företag möjligheten att koda direkt på sitt utvecklingskluster.

Submitted by PPG Educa??o em Ci?ncias e Matem?tica (educem-pg@pucrs.br) on 2018-06-27T13:27:54Z No. of bitstreams: 1 Disserta??o Vanessa Mendes de Lima-homologada-26-06-18.pdf: 1584014 bytes, checksum: efff3675beca980f3daec511415ba17d (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-07-03T14:11:30Z (GMT) No. of bitstreams: 1 Disserta??o Vanessa Mendes de Lima-homologada-26-06-18.pdf: 1584014 bytes, checksum: efff3675beca980f3daec511415ba17d (MD5) / Made available in DSpace on 2018-07-03T14:34:17Z (GMT). No. of bitstreams: 1 Disserta??o Vanessa Mendes de Lima-homologada-26-06-18.pdf: 1584014 bytes, checksum: efff3675beca980f3daec511415ba17d (MD5) Previous issue date: 2018-03-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The following investigation aims to analyze how the skills and competencies of the PISA test questions reflect the reality of school science education in Porto Alegre and its metropolitan region. The subjects who took part in this research were three teachers from the Natural Sciences area and one hundred and nine students, aged 15 to 16 years old, from two public schools of Porto Alegre and one private school from its metropolitan region. For data collection instruments, we used questionnaires to collect their profiles; individual semi-structured interviews with teachers; semi-structured interviews in focus groups with students, and three questions, with different competencies from PISA, which were answered by the students of this research. We analyzed data collected qualitatively through Content Analysis, according to the assumptions of Bardin (2016). Analyzes compose a discussion about the proximities and disagreements between the dimensions of scientific literacy proposed by PISA and the reality of science education. In addition, they are intended to argue if the competences required by the evaluation meet scientific knowledge presented by the students taking part in this research. From the analysis of the results, it is possible to infer that the skills and competencies of the PISA questions are beyond the knowledge presented by the students; therefore, they do not reflect the reality of the science education of studied schools. / A presente investiga??o tem o objetivo de analisar como as compet?ncias e habilidades das quest?es da prova do PISA est?o refletidas na realidade da educa??o em ci?ncias de escolas de Porto Alegre e regi?o metropolitana. Os sujeitos participantes da pesquisa foram tr?s professores da ?rea de Ci?ncias da Natureza e cento e nove estudantes, na faixa et?ria de 15 a 16 anos, de duas escolas p?blicas de Porto Alegre e uma escola privada da regi?o metropolitana. Como instrumentos de coleta de dados foram utilizados question?rios, para levantamento de perfil; entrevistas semiestruturadas individuais, com os professores; entrevistas semiestruturadas em grupos focais, com os estudantes e tr?s quest?es, de compet?ncias distintas, do PISA, que foram respondidas pelos estudantes sujeitos da pesquisa. Os dados coletados foram analisados qualitativamente por meio do m?todo de An?lise de Conte?do, de acordo com os pressupostos de Bardin (2016). As an?lises comp?em uma discuss?o acerca das aproxima??es e dos desacordes entre as dimens?es do letramento cient?fico, propostas pelo PISA e a realidade da educa??o em ci?ncias. Al?m disso, consistem em argumentar se as compet?ncias exigidas pela avalia??o v?o ao encontro dos conhecimentos cient?ficos apresentados pelos estudantes participantes desta pesquisa. A partir da an?lise dos resultados, ? poss?vel inferir que as compet?ncias e habilidades das quest?es do PISA est?o al?m dos conhecimentos apresentados pelos estudantes, portanto, n?o est?o refletidas na realidade da educa??o em ci?ncias das escolas estudadas.

Thesis (M.A.)--香港中文大學. / Includes bibliographical references. / Thesis (M.A.)--Xianggang Zhong wen da xue. / Chapter 第一章 --- 研究目的、材料與方法 / Chapter 第一節 --- 研究目的 --- p.1-8 / Chapter 第二節 --- 研究材料 --- p.9-12 / Chapter 第三節 --- 研究方法 --- p.12-14 / 註1至註9 --- p.15-19 / Chapter 第二章 --- 清戈載〈詞林正韻〉 / Chapter 第一節 --- 〈正韻〉一書之製作目的 --- p.20 / Chapter 第二節 --- 〈正韻〉一書之體例 --- p.21-23 / Chapter 第三節 --- 〈詞林正韻〉之分部系統 --- p.23-34 / Chapter 第四節 --- 〈正韻〉照顧方音及古今音變之特色 --- p.34-52 / Chapter 第五節 --- 關於葉斐軒刊本〈詞林韻釋〉 --- p.53-56 / 註1至註9 / Chapter 第三章 --- 〈詞林韻釋〉分部內容之修正 / Chapter 第一節 --- 統計及推論 --- p.60-65 / Chapter 第二節 --- 支持〈灰韻應歸第五部〉之兩項事實 --- p.65-71 / Chapter 第三節 --- 探討〈正韻〉歸部錯誤之原因 --- p.71-73 / 註1至註4 --- p.74 / Chapter 第四章 --- 論宋詞人有「平聲貴嚴」之觀念 --- p.75-79 / Chapter 第五章 --- 方音 / Chapter 第一節 --- 略論研究方音及決定其為方音之條件 --- p.81-85 / Chapter 第二節 --- 材料所示宋代之方音概況 --- p.86-103 / 註1至註6 --- p.104-105 / Chapter 第六章 --- 入聲 / Chapter 第一節 --- 〈詞林正韻〉入聲分部的特點 --- p.106-112 / Chapter 第二節 --- 個別詞家的入聲分部系統 --- p.113-138 / Chapter 第三節 --- 探討〈正韻〉入聲歸入三聲之根據 --- p.138-144 / 註1至註3 --- p.144A / Chapter 第七章 --- 宋詞之特殊協韻技巧 / Chapter 第一節 --- 不自覺的協韻方式 --- p.145-151 / Chapter 第二節 --- 長尾韻 --- p.151-154 / Chapter 第三節 --- 句中韻 --- p.154-156 / Chapter 第四節 --- 和聲 --- p.157-158 / Chapter 第五節 --- 福唐獨木橋體 --- p.158-161 / 註1 --- p.161 / Chapter 附錄一 --- 〈詞林正韻〉補正 / Chapter 附錄二 --- 四十二詞家曾使用之韻字 / Chapter 附錄三 --- 有關本文修正〈正韻〉分部內容之資料備矤 （本文第三章第一節表中II、IV、V、VI各項之內容） / Chapter 附錄四 --- 有關本文第四章之資料備攷 / Chapter 附錄五 --- 有關本文第五章「方音」之詳細資料 / Chapter 附錄六 --- 平仄通協體 / Chapter 附錄七 --- 詩韻詞韻分合對照表 / Chapter 附錄八 --- 詞韻分部索引表 （以〈廣韻〉韻目為主）

徐笑珍. / "2002年6月" / 論文 (哲學碩士)--香港中文大學, 2002. / 參考文獻 (leaves 135-141) / 附中英文提要. / "2002 nian 6 yue" / Xu Xiaozhen. / Lun wen (zhe xue shuo shi)--Xianggang Zhong wen da xue, 2002. / Can kao wen xian (leaves 135-141) / Fu Zhong Ying wen ti yao. / Chapter 第一章 --- 緒論 / Chapter 第一節 --- 硏究背景及槪況 --- p.1-2 / Chapter 第二節 --- 硏究目的 --- p.3 / Chapter 第三節 --- 硏究方法 --- p.4-6 / Chapter 第二章 --- 夏承燾的生平事蹟 / Chapter 第一節 --- 夏承燾的治詞經歷 --- p.7-16 / Chapter 第二節 --- 夏承燾的性情 --- p.17-28 / Chapter 第三節 --- 夏承燾的交遊 / Chapter 一、 --- 夏承燾交遊述介 --- p.29-37 / Chapter 二、 --- 夏承燾詞友考析 --- p.38-42 / Chapter 第四節 --- 小結 --- p.43 / Chapter 第三章 --- 夏承燾的詞學管窺 / Chapter 第一節 --- 譜牒之學 / Chapter 一、 --- 編纂方法及動機 --- p.44-45 / Chapter 二、 --- 資料取材 --- p.46-47 / Chapter 三、 --- 編纂凡例 --- p.47-55 / Chapter 四、 --- 夏氏所編年譜的得失 --- p.56-59 / Chapter 第二節 --- 校勘箋注之學 / Chapter 一、 --- 《姜白石詞編年箋校》 --- p.60-62 / Chapter 二、 --- 《龍川詞校箋》 --- p.62-63 / Chapter 三、 --- 《宋詞繫》 --- p.63-65 / Chapter 四、 --- 《詞源注》 --- p.65-66 / Chapter 第三節 --- 詞樂之學 / Chapter 一、 --- 宮調 --- p.67 -69 / Chapter 二、 --- 旁譜 --- p.69-71 / Chapter 第四節 --- 詞籍編纂之學 --- p.72-73 / Chapter 第五節 --- 批評之學 / Chapter 一、 --- 詩話式評點´ؤ´ؤ《瞿髯論詞絶句》 --- p.74 -87 / Chapter 二、 --- 短篇式評點´ؤ´ؤ《月輪山詞論集》 --- p.87 -89 / Chapter 三、 --- 考訂式評點´ؤ´ؤ《唐宋詞論叢》 --- p.89 -90 / Chapter 四、 --- 賞析性評點´ؤ´ؤ《唐宋詞欣賞》 --- p.90 -91 / Chapter 第六節 --- 小結 --- p.92-93 / Chapter 第四章 --- 夏承燾的詞作探析 / Chapter 第一節 --- 內容主題 --- p.94-107 / Chapter 第二節 --- 藝術表現 --- p.108-116 / Chapter 第三節 --- 風格特點 --- p.117-128 / Chapter 第四節 --- 小結 --- p.129 / Chapter 第五章 --- 總結 --- p.130-134 / 參考書目及論文 --- p.135-141 / 附表一：夏承燾年譜簡表 --- p.142-162 / 附表二 ：夏承燾交遊表 --- p.163-184 / 附表三：夏承燾詞作內容分類表 --- p.185-196

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2015-11-03T13:22:19Z No. of bitstreams: 1 475963 - Texto Completo.pdf: 2884204 bytes, checksum: 89d26683140d05763c64feba473092cb (MD5) / Made available in DSpace on 2015-11-03T13:22:19Z (GMT). No. of bitstreams: 1 475963 - Texto Completo.pdf: 2884204 bytes, checksum: 89d26683140d05763c64feba473092cb (MD5) Previous issue date: 2015-08-24 / The rearrangements of the capital system, faced with yet another of its cyclical crises triggered starting at the end of the 20th century and the beginning of the 21st, has brought to vogue the theme of cooperativism as a possibility for absorbing the workforce that is unnecessary to the formal employment mechanisms. Allied to this strategy of the system, anti-systemic movements have appeared that postulated alternative escapes to the working class so that these would turn into instruments for overcoming the logic of capital. Thus enter the scenario the enterprises of Social and Solidary Economy with a proposal of new sociability, based on the logic of cooperative production with self-managerial principles capable of producing the emancipation of freely associated workers. What has been the role of the State, in this historical period, in which public policies focus on the capital system or are formulated for this new "form of producing" life? The challenge of building a new cooperative model, ideologically and politically distinct from the traditional cooperativism and which, in a way, resizes the logic of associated work, puts itself in the everyday life of workers inthe enterprise of Social and Solidary Economy in the whole world, and more specifically, in Brazil. In times of re-structuring of capitalism, the alternatives can constitute strategies of reframing of the work as a development potential of the "New Man". / Os rearranjos do sistema do capital em face demais uma das suascrises c?clicasdesencadeadas a partir do final do s?culo XX e in?cio do XXI, trouxe ? voga o tema do cooperativismo como possibilidade de absor??o da m?o de obra desnecess?ria aos mecanismosformais de trabalho. Aliado a esta estrat?gica do sistema sugiram movimentos antissist?micos que postulavam sa?das alternativas para a classe trabalhadora que se transformassem em instrumentos de supera??o da l?gica do capital. Entramno cen?rio os empreendimentos da Economia Social e Solid?ria com uma proposta de nova sociabilidade, calcada na l?gica da produ??o cooperativista com princ?pios autogestion?rios capazes de produzir a emancipa??o dos trabalhadores livremente associados. Como tem sido o papel do Estado, neste per?odo hist?rico, em que pol?ticas p?blicas incidem no sistema do capital ou s?o formuladas para esse novo ?modo de produzir? a vida?.O desafio de construir um modelo de cooperativismo distinto, ideol?gicoe politicamente do cooperativismotradicional e que, de certa forma, redimensione a l?gica do trabalho associado colocando-seno cotidiano dos trabalhadores dos empreendimentos da Economia Social e Solid?ria em todo o mundo, e no caso espec?fico, no Brasil. Em tempos de reestrutura??o do capitalismo, as alternativas podem constituir-se em estrat?gias de resignifica??o do trabalho como potencial de desenvolvimento do ?Homem Novo?.

Submitted by Setor de Tratamento da Informa??o - BC/PUCRS (tede2@pucrs.br) on 2016-08-04T16:55:59Z No. of bitstreams: 1 DIS_FABIAN_SICHONANY_SAMUEL_COMPLETO.pdf: 736237 bytes, checksum: 6e884f9a6210c883e1d869565c0ad349 (MD5) / Made available in DSpace on 2016-08-04T16:55:59Z (GMT). No. of bitstreams: 1 DIS_FABIAN_SICHONANY_SAMUEL_COMPLETO.pdf: 736237 bytes, checksum: 6e884f9a6210c883e1d869565c0ad349 (MD5) Previous issue date: 2016-04-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Funda??o de Amparo ? Pesquisa do Estado do Rio Grande do Sul - FAPERGS / This work aims to contribute to studies on the subject of volunteering, given the importance that the movement is gaining in Brazil, especially after the period following the re-democratization of the country. At first, it was analyzed the development of the activity in Brazil and its social implications, particularly as regards the qualifications of social ties in the face of growing individualism. So, given the importance of this activity, in a second step, still in a theoretical way, the analysis focused on the social actions and how they are produced. For this purpose, it was used the theory of Alfred Schutz action. It is understood, in this study, the engagement action in volunteering as a social action according to Schutz. This study sought to understand how the reasons and motives for engaging in volunteering are built through the life experiences of social actor, seeking to reveal the because motives (or biographic motives). This study conducted five interviews with people who volunteer in institutions of civil society, of which the analysis and presentation that were oriented by the method of Narrative Biographical developed by Fritz Sch?tze and perfected by Gabriele Rosenthal, focused on one of them. Empirical research with Fernanda has revealed that the engagement action in volunteering is constructed biographically through the relationship between the biographically determined situation and the world of common sense. Biographical experiences both change the interpretations of social actor on the world and guide him in the decision making process to engage in voluntary activity. In addition, we glimpse a singular biographical path, composed of four main biographical moments, interconnected, which, added to a context where the culture of volunteering is widespread, favored Fernanda on the occasion of his decision to become a volunteer. The biographee managed to turn the disease and suffering in personal growth factor, through the contact and socialization in a spiritist universe of meaning, and that was determinant for her in order to prepare her action project. / Este trabalho tem como objetivo principal contribuir com os estudos sobre a tem?tica do voluntariado, em face da import?ncia que o movimento vem ganhando no Brasil, sobretudo ap?s o per?odo que se seguiu a redemocratiza??o do pa?s. Foi analisado em um primeiro momento o desenvolvimento da atividade no Brasil e suas implica??es sociais, sobretudo no que se refere ? qualifica??o dos la?os sociais em face do individualismo crescente. Ent?o, tendo em vista a import?ncia desta atividade, em um segundo momento a analise recaiu, ainda de maneira te?rica, sobre as a??es sociais e como s?o produzidas. Valeu-se para tanto, da teoria da a??o de Alfred Schutz. Entende-se, neste estudo, a a??o de engajamento no voluntariado como uma a??o social ? maneira do referido autor. Este trabalho buscou compreender como as raz?es e os motivos para o engajamento no voluntariado s?o constru?dos atrav?s das experi?ncias de vida do ator social, buscando com isso revelar os motivos porqu? (ou motivos biogr?ficos). Neste estudo foram realizadas cinco entrevistas com pessoas que realizam atividade volunt?ria em institui??es da sociedade civil, das quais a an?lise e a apresenta??o, que foram orientadas segundo o m?todo da Narrativa Biogr?fica, desenvolvido por Fritz Sch?tze e aperfei?oado por Gabriele Rosenthal, recaiu sobre uma delas. A pesquisa emp?rica com Fernanda nos revelou que a a??o de engajamento no voluntariado ? constru?da biograficamente atrav?s da rela??o que existe entre a situa??o biograficamente determinada e o mundo de sentido comum. As experi?ncias biogr?ficas tanto modificam as interpreta??es do ator social sobre o mundo, como o orientam na tomada de decis?o no sentido de engajar-se no voluntariado. Ademais, vislumbramos um caminho biogr?fico singular, composto de quatro momentos biogr?ficos principais, interconectados, e que, somados a um contexto onde a cultura sobre o voluntariado ? amplamente difundida, favoreceram Fernanda por ocasi?o de sua decis?o de se tornar uma volunt?ria. A biografada conseguiu transformar a doen?a e o sofrimento em fator de crescimento pessoal, a partir do contato e da socializa??o em um universo de significado esp?rita, e isso foi determinante para que elaborasse o seu projeto de a??o.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-05T12:02:02Z No. of bitstreams: 1 2010 - Cesar Daniel Kruger.pdf: 4305938 bytes, checksum: b0a2db06d6603a1f3b55d1c934d1f418 (MD5) / Made available in DSpace on 2016-08-05T12:02:02Z (GMT). No. of bitstreams: 1 2010 - Cesar Daniel Kruger.pdf: 4305938 bytes, checksum: b0a2db06d6603a1f3b55d1c934d1f418 (MD5) Previous issue date: 2010-02-11 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES / Interest in fungi and the mycotoxins is enormous, not only from the scientific point of view, but also in the economic outlook. There are many problems arising from the farmer to the consumer. Low yields, deteriorating levels in commercial husbandry of animals and disease in them, changes in food, loss of sensory and nutritional characteristics, increased costs resulting from the prevention and treatment of decontamination are among some of the disorders generated by the presence of fungi and their matab?litos. Ochratoxin A occupies every day a major role in the setting of Mycotoxicology due to its importance. Many efforts have been undertaken to define the global situation of occurrence of this mycotoxin. This study focused on a set in specific areas the occurrence of this mycotoxin, which not long ago was ignored in the setting of contaminants in Brazil as well as putting into practice the methods of detection of OTA in pig serum. We collected 400 samples of blood serum from pig slaughterhouses in four different states. These samples were processed and subjected to techniques for determining liquid chromatography with high efficiency to determine concentrations of the toxin in the study. RESULTS: The mean concentration of ochratoxin A in pig blood serum samples with levels above the limit of detection was 23.729 ?g/L. CONCLUSIONS: Ochratoxin A is present in the pork produced in Brazil. All the samples surveyed were above the limit of detection and quantification. The colder climate favors the development of ochratoxin A, demonstrated the highest level of positive samples with limits above 5 ?g/L in the State of Santa Catarina (52%). The state of Mato Grosso showed the highest number of positive samples for ochratoxin A above the limit detectable by the technique used (69%). The State of Bahia showed the greatest number of samples below the detectable limit (64%). / O interesse pelos fungos e pelas micotoxinas ? enorme, n?o s? do ponto de vista cient?fico, como tamb?m sob a perspectiva econ?mica. S?o muitos os problemas gerados desde o agricultor at? o consumidor final. Baixos rendimentos da colheita, deterioramento de n?veis zoot?cnicos na produ??o comercial de animais e doen?a nos mesmos, altera??es nos alimentos, perdas de caracter?sticas sensoriais e nutricionais, aumento dos custos derivados da preven??o e do tratamento descontaminante est?o entre alguns dos transtornos gerados pela presen?a dos fungos e seus matab?litos. A ocratoxina A ocupa a cada dia um papel de destaque no cen?rio da micotoxicologia devido a sua import?ncia. Muitos esfor?os t?m sido empreendidos para se definir a situa??o mundial da ocorr?ncia desta micotoxina. Este trabalho teve como foco principal definir em algumas ?reas espec?ficas a ocorr?ncia desta micotoxina, que h? pouco tempo atr?s era ignorada dentro do cen?rio dos contaminantes nos produtos brasileiros, al?m de colocar em pr?tica a metodologia de detec??o de OTA em soro su?no. Foram coletadas 400 amostras de soro sangu?neo su?no em diferentes matadouros de quatro estados brasileiros. Estas amostras foram processadas e submetidas a t?cnicas de determina??o por cromatografia l?quida de alta efici?ncia para se determinar suas concentra??es da toxina em estudo. RESULTADOS: A concentra??o m?dia de ocratoxina A no soro sangu?neo su?no das amostras com n?veis acima do limite de detec??o foi de 23,729 ?g/L. CONCLUS?ES: A ocratoxina A est? presente no su?no produzido no Brasil. Todos os Estados pesquisados apresentaram amostras acima do limite de detec??o e quantifica??o. O clima mais frio favorece o aparecimento de ocratoxina A, demonstrado no maior n?vel de amostras positivas com limites acima de 5 ?g/L no Estado de Santa Catarina (52%). O Estado de Mato Grosso apresentou o maior n?mero de amostras positivas para ocratoxina A acima do limite detect?vel pela t?cnica utilizada (69%). O Estado da Bahia foi o que apresentou maior quantidade de amostras abaixo do limite detect?vel (64%).

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-28T15:23:40Z No. of bitstreams: 1 2011 - Nathanielly Rocha Casado de Lima.pdf: 972650 bytes, checksum: 29dba077fa4bb5ed1cb4977f2f174d44 (MD5) / Made available in DSpace on 2016-09-28T15:23:40Z (GMT). No. of bitstreams: 1 2011 - Nathanielly Rocha Casado de Lima.pdf: 972650 bytes, checksum: 29dba077fa4bb5ed1cb4977f2f174d44 (MD5) Previous issue date: 2011-03-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The Trypanosomatidae family includes parasites of a wide variety of vertebrates, invertebrates (mainly insects), plants and some species of protozoa. In this study, an isolate was obtained by feces culturing of the intestinal tract of Megaselia scalaris Loew, 1866 (Diptera, Phoridae). The original isolate and its clones (obtained by flow cytometry) were deposited in the ?Trypanosomatids Collection of the Instituto Oswaldo Cruz. These clones were characterized by different approaches I in comparison with the reference species of different genera. Its growth has been studied in LIT medium (with 10 or 20% fetal bovine serum) modified according to the requirement. The number of cells per microliter was estimated at 24 h intervals between 48-144 h. Giemsa-stained smears (after acid hydrolysis) were prepared in order to study cell differentiation and morphology of the forms. The isozyme analysis was performed using the following systems: GPI, PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI and HK. Data from this analysis were processed and analyzed numerically using the computacional Jaccard coefficient and UPGMA algorighm. The result set of this study suggest that: (1) The strain of M. scalaris showed 70% similarity with Herpetomonas megaseliae, (2) This isolate showed the exclusive presence of promastigotes in any analysis of the growth curve as expected for the genus Leptomonas, however the forms exhibited high polymorphism. Additional studies using molecular tools will be realized / A fam?lia Trypanosomatidae inclui parasitas de uma grande variedade de vertebrados, invertebrados (principalmente insetos), plantas e algumas esp?cies de protozo?rios. No presente trabalho, um isolado foi obtido por coprocultivo de trato intestinal de Megaselia scalaris Loew, 1866 (Diptera, Phoridae). O isolado original e seus clones (obtidos por citometria de fluxo) foram depositados na "Cole??o de Tripanosomat?deos do Instituto Oswaldo Cruz". Estes clones foram caracterizados por diversas abordagens em compara??o com esp?cies de refer?ncia de diferentes g?neros. Seu crescimento foi estudado em meio LIT (com 10 ou 20% de soro fetal bovino) modificado, de acordo com o n?vel de exig?ncia. O n?mero de c?lulas por microlitro foi estimado em intervalos de 24 h, entre 48-144 h. Esfrega?os corados pelo Giemsa (ap?s hidr?lise ?cida) foram preparados visando estudos de diferencia??o celular e morfometria das formas encontradas. A an?lise de isoenzimas foi realizada utilizando-se os seguintes sistemas: GPI , PGM, 6PGDH, ME, IDH, MDH, ACON, FUM, MPI e HK. Os dados desta an?lise foram processados numericamente e submetidos ? an?lise computacional utilizando-se o coeficiente de Jaccard e o algoritmo UPGMA. O conjunto de resultados deste trabalho sugere que: (1) O isolado de M. scalaris apresentou similaridade de 70% com Herptomonas megaseliae; (2) Esse isolado revelou a presen?a exclusiva de promastigotas em toda an?lise da curva de crescimento, como esperado para o g?nero Leptomonas, entretanto as formas exibiram grande polimorfismo. Estudos complementares utilizando ferramentas moleculares ser?o realizados.