Made available in DSpace on 2014-12-17T14:14:03Z (GMT). No. of bitstreams: 1 WaleriaCMM_DISSERT.pdf: 1244886 bytes, checksum: c6bc196c2f831dc8cda208bb1d204162 (MD5) Previous issue date: 2012-12-21 / Funda??o de Apoio ? Pesquisa do Estado do Rio Grande do Norte / A reabsor??o e a forma??o ?ssea s?o processos fisiologicamente independentes, sendo que o predom?nio de um sobre o outro pode resultar em perda ou ganho de massa ?ssea. A perda gradual do col?geno surge como um dos principais fatores apresentado por mulheres em fase de climat?rio, interferindo negativamente na qualidade de vida. O objetivo do presente estudo foi identificar as concentra??es s?ricas do C- Telopept?deo de col?geno (CTX) e correlacionar sua ocorr?ncia com os n?veis hormonais do Estradiol (E2), Testosterona (T) e IMC em mulheres na fase de vida reprodutiva e na p?s-menopausa. Estudo transversal, onde as concentra??es s?ricas do CTX foram dosadas em 41 mulheres alocadas intencionalmente no ambulat?rio da Maternidade Escola Janu?rio Cicco (MEJEC) da Universidade Federal do Rio Grande do Norte (UFRN), pelo m?todo de eletroquimiluminesc?ncia estratificadas em: 11 mulheres com ciclos ovulat?rios regulares confirmados pela dosagem de progesterona na fase l?tea do ciclo no 21? com idades entre 20 e 30 anos (GI) e 30 mulheres na p?s-menopausa com fal?ncia ovariana, comprovada pelos n?veis do Horm?nio Fol?culo Estimulante (FSH) > 30 UI/ml e n?veis de E2 > 40 UI/ml h? pelo menos um ano com idades entre 46 e 60 anos (GII) com uma subdivis?o de acordo com o ?ndice de massa corp?rea (IMC) em: normal: (n=11), sobrepeso (n=14) e obesidade (n=5). As an?lises estat?sticas foram determinadas pelos testes de Mann Whitney, Kruskal Wallis e correla??o de Spearman. Os resultados mostraram que n?o houve diferen?a entre os grupos estudados (p=0,717), como tamb?m n?o houve correla??o significativa entre eles para o CTX, E2, T e IMC. Neste estudo, n?o houve correla??o entre os n?veis do CTX, IMC e n?veis hormonais em mulheres na pr? e p?s menopausa. Estudos futuros com outros delineamentos longitudinais poder?o apresentar outras estimativas

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-01-23T14:20:39Z No. of bitstreams: 1 ElisangelaBezerraDasNevesHolanda_TESE.pdf: 6612707 bytes, checksum: f9d2acfe515476ab9c03e0ac3ce7b4c2 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-01-24T15:42:12Z (GMT) No. of bitstreams: 1 ElisangelaBezerraDasNevesHolanda_TESE.pdf: 6612707 bytes, checksum: f9d2acfe515476ab9c03e0ac3ce7b4c2 (MD5) / Made available in DSpace on 2018-01-24T15:42:12Z (GMT). No. of bitstreams: 1 ElisangelaBezerraDasNevesHolanda_TESE.pdf: 6612707 bytes, checksum: f9d2acfe515476ab9c03e0ac3ce7b4c2 (MD5) Previous issue date: 2017-08-14 / Os nanotubos de carbono de multicamadas (MWCNT) incorporados ? materiais de origem proteicas podem contribuir para o surgimento de interessantes biomateriais comp?sitos com grande potencial de aplica??es biol?gicas. Os MWCNT s?o promissores vetores de DNA em c?lulas e embri?es de seres vivos. Por?m, o desencadeamento do seu uso na ?rea biom?dica est? relacionado ? padroniza??o de sistemas e protocolos para avaliar os potenciais impactos na sa?de humana e no meio ambiente. O objetivo geral deste estudo foi avaliar o efeito da funcionaliza??o, a citotoxicidade e a ades?o celular dos nanotubos de carbono na sua forma comercial e modificados por diferentes processos em c?lulas macr?fagos (J774 e RAW). Nesta pesquisa, inicialmente foram realizados diferentes processos de funcionaliza??o nos MWCNT visando a inser??o de grupos funcionais e poss?vel compatibilidade com biopol?meros. Ap?s a funcionaliza??o os MWCNT foram caracterizados por diferentes t?cnicas e realizados os ensaios de citotoxicidade e a eletroan?lise. Os biopol?meros utilizados para a funcionaliza??o do substrato t?xtil da prote?na da soja foram o col?geno extra?do da pele da til?pia e o col?geno comercial. Os substratos t?xtis foram funcionalizados atrav?s do processo Dip coating e em seguida, foram incorporados os nanotubos de carbono pelo mesmo processo. Os resultados obtidos atrav?s das an?lises do processo de funcionaliza??o dos MWCNT revelaram que as amostras funcionalizadas com a quitosana tende a aumentar o di?metro do tubo devido a forma??o de uma monocamada polim?rica em sua superf?cie e que os melhores resultados de oxida??o foram para as amostras que passaram pelo processo de funcionaliza??o ?cida e funcionaliza??o ?cida com quitosana. Os resultados obtidos pela Espectroscopia Raman comprovaram que o processo de funcionaliza??o foi realizado com ?xito, possibilitando a inser??o de grupos funcionais sobre os nanotubos. A eletroan?lise nos nanotubos mostraram que os maiores valores da capacit?ncia por massa e por ?rea foram para as amostras funcionalizadas com ?cidos e que a adi??o da quitosana durante a funcionaliza??o aumenta a resist?ncia ? passagem da corrente, al?m de provocar um efeito isolante. Os ensaios de citotoxicidade dos MWCNT realizadas em macr?fagos nas concentra??es de 100, 75, 50, 25 e 5 ?g/mL demonstraram um percentual em torno de 95% de viabilidade celular para as amostras funcionalizadas com ?cido e quitosana para todas as concentra??es. Os ensaios de ades?o celular nos MWCNT revelaram excelentes intera??es em concentra??es mais elevadas para todas as amostras de nanotubos. A observa??o visual do efeito da modifica??o do substrato t?xtil com o col?geno indicou que o tipo e a origem da prote?na interferem no efeito da imobiliza??o dos nanotubos de carbono. Em conclus?o, as exposi??es de macr?fagos a baixas concentra??es de MWCNT funcionalizados n?o causaram impacto na viabilidade celular, o que abre as possibilidades de diversos estudos para potencializar aplica??es na ?rea biol?gica. / The multiwalled carbon nanotubes (MWCNT) incorporated into the materials of protein origin can contribute to the emergence of interesting composite biomaterials with great potential of biological applications. MWCNT are promising DNA vectors in living cells and embryos. However, the triggering of its use in the biomedical area is related to the standardization of systems and protocols to assess the potential impacts on human health and the environment. The general objective of this study was to evaluate the effect of functionalization, cytotoxicity and cellular adhesion of carbon nanotubes in their commercial form and modified by different processes in macrophages (J774 and RAW). In this research, different functionalization processes were initially performed in the MWCNT, aiming at the insertion of functional groups and possible compatibility with biopolymers. After functionalization, the MWCNT were characterized by different techniques and performed the cytotoxicity and electroanalysis tests. The biopolymers used for the functionalization of the textile substrate of the soy protein were the collagen extracted from the skin of the tilapia and the commercial collagen. The textile substrates were functionalized through the dip coat process and then the carbon nanotubes were incorporated by the same process. The results obtained through the analysis of the functionalization process of the MWCNT revealed that the functionalized samples with chitosan tend to increase the diameter of the tube due to the formation of a polymeric monolayer on its surface and that the best oxidation results were for the samples that passed by the process of acid functionalization and acid functionalization with chitosan. The results obtained by Raman Spectroscopy proved that the functionalization process was successful, allowing the insertion of functional groups on the nanotubes. The electroanalysis in the nanotubes showed that the highest values of capacitance per mass and per area were for the acid functionalized samples and that the addition of chitosan during functionalization increases the resistance to current flow, besides provoking an insulating effect. The cytotoxicity tests of MWCNT performed on macrophages at concentrations of 100, 75, 50, 25 and 5 ?g / mL demonstrated a percentage of around 95% cell viability for acid and chitosan functionalized samples at all concentrations. Cell adhesion assays in MWCNTs showed excellent interactions at higher concentrations for all nanotube samples. Visual observation of the effect of modification of the textile substrate with collagen indicated that the type and origin of the protein interfere with the immobilization effect of the carbon nanotubes. In conclusion, macrophage exposures at low concentrations of functionalized MWCNT had no impact on cell viability, which opens the possibilities of several studies to potentiate applications in the biological field.

Purpose: The main purpose of this study/thesis is to, through an interview with researchers in medical field in Jönköping,  provide an empirical analysis of the link or relationship between medical researcher and patient through social networking sites specifically for collaboration in order to improve relationships, dissemination of information and knowledge sharing. Background: The importance of social networking websites as a means of interaction between groups of individuals cannot be underestimated. Their impact on daily life activities and activities cannot be underestimated. Because  millions of individuals are making use of Social Network Sites (SNSs) to build or reinforce relationships, connect, disseminate and share information as well as sharing of knowledge whether personal or non-personal experiences with people they already know offline or new people online. Therefore, the authors are interested basically in how social networking web sites are being utilized in terms of collaborations, information and knowledge sharing and particularly in what benefits and challenge are connected to improving inter-groups collaboration in research study between researchers and ordinary citizens. Method: A review of literature gives us insight about the subject terms, critical and sensitive issues in regards to collaboration through social networking sites designed for research purpose. We apply in general networking theories such as social capital and two of its components couple with Putman´s theory of bonding and bridging social capital as a theoretical framework to synthesize the concept of  ties (strong or weak) . Our analysis based on the empirical data gathered through surveys, interviews and observation provide us with interesting preliminary results and with blueprints to guide the analysis of the thesis. Conclusion: Social networking platforms are valuable and useful in our generation being part of daily life and activity to keep up with people within our networks. However, the authors discovered that the advantages of involving ordinary citizens to participate in improving researcher-patient collaboration through dynamic social networking actually outweigh the disadvantages despite all odds and sensitive issues such as trust, privacy issues and sceptics of some researchers. In others words, some of the researchers were enthusiastic to collaborate in terms of disseminating useful information and sharing valuable knowledge with ordinary citizens. Overall, the study revealed positive result that despite weak ties relationships, the willingness to collaborate is far more than the obstacles perceived in the course of establishing such collaboration.

Le cancer du col de l'utérus est le troisième cancer le plus fréquent chez la femme dans le monde. Cependant l'efficacité des traitementsconventionnels n'est pas toujours optimale. La recherche de nouvelles molécules naturelles, possédant des propriétés anticancéreuses et n'induisantpas d'effets secondaires, est donc d'un intérêt majeur. Parmi ces molécules, un flavonoïde se distingue des autres par ses nombreux effetsthérapeutiques potentiels, l'isoliquiritigénine (ILG). Cette chalcone naturellement présente dans la racine de réglisse, possède de nombreusespropriétés, incluant une activité anti-tumorale.Le but de cette thèse était d'étudier l'effet anticancéreux de ITLG synthétique dans des cellules dérivées de cancer du col de l'utérus.L'étude du mécanisme moléculaire impliqué dans Papoptose a été centrée sur les cellules les plus représentatives des carcinomes du col de l'utérus,les Ça Ski infectées par le papillomavirus de type 16 dans lequel nous démontrons que l'ILG inhibe l'expression de l'oncogène viral E6 d'HPVlô.Nos résultats montrent que l'ILG diminue la viabilité, induit un arrêt de cycle et favorise l'apoptose dans des cellules dérivées de cancer du col del'utérus différant par leur statut viral et leur statut p53.Nous mettons ainsi en évidence que l'apoptose induite par l'ILG est spécifique des cellules tumorales et implique différentes voies de signalisationsuivant le type cellulaire. L'ILG pourrait donc faire partie d'un nouvel arsenal thérapeutique visant à lutter contre le cancer du col de l'utéru / Cervix cancer is the third most common cancer among women Worldwide. Many treatments are already available but they are not always efficient.This is why new natural molecules with anticancer properties that could reduce the side effects of conventional therapy are investigated. Among them,isoliquiritigenin (ILG), a fiavonoid, differentiates itself through ils various potential therapeutic properties. This chalcone isolated from roots oflicorice has many properties including an anti-tumor activity.The aim of this thesis was to study anti-tumoral effects of synthetic ILG in cervical cancer cells.We show that ILG decreases viability, induces cell cycle arrest and engages apoptosis in cervial cancer cells differing one from the other by their viraland p53 status. The death induced by ILG in the HPV positive and wild-type p53 Ça Ski, SiHa and HeLa cells appears to be independant of p53 andof the mitochondria, contrary to what happens in the HPV negative mutated p53 C-33A cells, where death could be initiated by an endoplasmicreticulum stress. The molecular mechanism of apoptosis is mainly studied on HPV 16 Ça Ski cells, the most representative cells of cervical carcinoma.ILG leads to a down-regulation of the viral oncogen E6 HPV16 expression.The study of the pathways involved by ILG highlights differences according to the cellular type. As it specifically induces apoptosis on tumoral cells,ILG could act as a potential therapeutic agent in cervix cancer.

京都大学 / 新制・課程博士 / 博士(医学) / 甲第24197号 / 医博第4891号 / 新制||医||1060(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 平井 豊博, 教授 万代 昌紀, 教授 伊達 洋至 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DGAM

Cette étude cas-témoins évalue l'impact de l'utilisation des services de santé et des caractéristiques sociodémographiques sur le risque d'être diagnostiqué avec un cancer du col utérin. L'utilisation des services de santé dans les 10 années antérieures au diagnostic a été analysée pour 3 005 Québécoises diagnostiquées et 8 762 témoins. La défavorisation matérielle et sociale accroît le risque de développer la maladie respectivement de 17% et 19% tandis que chaque visite annuelle supplémentaire chez un médecin de famille et chez un obstétricien-gynécologue réduit le risque respectivement de 2% et 16%. Toutefois, c'est la continuité dans les soins qui ressort comme un déterminant majeur : avoir une continuité de niveau moyen accroît le risque de 21% et une faible continuité, de 28%. Néanmoins, les trois quarts des femmes diagnostiquées ont vu un médecin de famille en moyenne au moins deux fois par année et la majorité ont une relation à long terme avec un médecin régulier.

No description available.

Les tueries de masse, de par leur caractère extraordinairement violent, provoquent l’indignation de la population. Pour des individus, ces évènements suscitent plutôt un sentiment de curiosité, lequel transite parfois vers l’admiration des auteurs de ces drames et à l’identification envers ceux-ci. Sur la toile, ces personnes se joignent pour former un groupe que l'on peut qualifier de « fandom ». Ce mémoire s’intéresse à ce phénomène qui,bien qu’il suscite la crainte chez la population, reste à ce jour peu exploré. Ainsi, l’étude vise à pousser l’exploration plus loin. Contrairement aux études précédentes, qui se sont davantage penchées sur les particularités individuelles, la recherche qui suit vise àcomprendre la façon le fandom centré sur les tueries de masse s’organise en communautés virtuelles. Pour y parvenir, la recherche a examiné, à l’aide de l’analyse thématique, les discussions tenues par les membres de la communauté r/Columbine, un site hébergé parla plateforme Reddit. Les discussions analysées ont pris place sur une période d’environ un an, la dernière publication ayant été publiée en janvier 2019. Les résultats suggèrent que, un peu à la façon des fandoms plus classiques, la communauté s’approprie, transforme etré interprète le narratif officiel pour créer son propre narratif à propos des tueurs. Ce narratif propre à la communauté r/Columbine semble être influencé par la subjectivité et les expériences de vie propres aux membres qui la composent. Consciente du regard externe porté à son endroit, la communauté négocie sa propre déviance en opposant son identité à celles des communautés qu’elle juge « plus déviantes » qu’elle. De cet exercice naît également un autre narratif, cette fois-ci à propos de l’identité de la communauté. / Due to its extreme violent nature, mass shootings provoke indignation within society. Tosome people, those events rather generate curiosity, which sometimes evolve toward identification to and admiration of the perpetrators of these crimes. Those people reach toeach other through the internet to form a group called « fandom ». This work wishes to explore that phenomena, which has been poorly studied up to now. Unlike previous studies, which have focused on individual peculiarities, this research aims to understand how the fandoms centered on mass shootings are organized into virtual communities. To achieve this objective, the research studied, through thematic analysis, the discussions held by the members of r/Columbine, a community hosted on Reddit. The analyzed discussion took place over a period of approximately one year, the last discussion having been published in January 2019. Results suggest that, in a way similar to the mainstream fandoms, the community appropriates, transforms and reinterprets the official narrative to create its own narrative about the shooters. This narrative specific to the r/Columbine community seem to be influenced by the subjectivity and the life experiences of its members. Aware of the way society sees them, community negotiate its own deviance by opposing its identity to those of the communities it deems ‘’more deviant’’. This exercise generates a second narrative concerning the community’s identity.

Lipid transfer proteins (LTPs) constitute a pervasive class of small proteins implicated in many biological and physiological processes, including seed development, germination, cuticle formation, and abiotic stress responses. In this study, we explored the role of Arabidopsis LTP12 protein in mitigating environmental stresses. To address this, we analyzed the T-DNA knockout mutant ltp12, focusing on its responses to salinity and osmotic stress. Utilizing antioxidant enzyme assays, phenotypic analyses (including water loss, chlorophyll content, seed germination rates, root length, and relative water content), and lipid profile analysis via Thin Layer Chromatography (TLC), we found that ltp12 mutants showed reduced catalase and peroxidase activities and poorer hydration, chlorophyll content, germination, and growth under stress, compared to wild-type Col-0 plants. Mutants delayed vegetative-to-floral phase transition compared to wild-type. Additionally, lipid analysis indicated that the wild-type plants had increased phospholipids under high osmotic stress, suggesting LTP12's involvement in lipid reorganization during stress responses.

Dans le présent mémoire, le potentiel d’un prototype de la sonde Clariry AutoScan modifiée pour la curiethérapie gynécologique est évalué. Au terme du projet, le but est de déterminer si la sonde peut être intégrée au processus clinique et si la planification de traitement faite sur image par tomodensitométrie (TDM) peut être améliorée par l’ajout d’une échographie transabdominale tridimensionnelle (3DTAUS). Pour ce faire, un total de 19 patientes ont été analysées au cours des différentes étapes du projet. La première partie, l’analyse des contours, comprenant une analyse de faisabilité a été réalisée. Dans cette section, différents éléments devaient être jugés visibles ou non sur les trois modalités d’imagerie (imagerie par résonnance magnétique (IRM), TDM et 3DTAUS) sur les images de 6 patientes. Par la suite, le volume cible clinique à hauts risque (High Risk Clinical Target Volume, HR-CTV) de 8 patientes a été délimité et une analyse comparative de volume et de dimensions a été réalisée. La dernière étape a été d’évaluer les plans de traitements optimisés sur IRM, TDM et TDM-3DTAUS réalisés pour 5 patientes. Pour fin de comparaison, les plans faits sur IRM ont été également évalués sur TDM et TDM-3DTAUS et les plans TDM et TDM-3DTAUS, sur l’IRM. L’analyse de faisabilité a montré que la sonde permet de voir le HR-CTV. Cependant, le rectum, le sigmoïde de même que les ovoïdes ne peuvent être visualisés sur le 3DTAUS. Pour les analyses de contours, en moyenne, les contours se rapprochant davantage du standard à atteindre, l’IRM, sont faits sur le 3DTAUS, mais avec une plus grande variation des contours que le TDM. Pour la planification de traitement, l’ajout du 3DTAUS au TDM permet d’obtenir une dosimétrie plus près de celle obtenue sur l’IRM comparativement au TDM seul. L’utilisation clinique du 3DTAUS est plausible. L’ajout du 3DTAUS permet d’obtenir une dosimétrie plus près de celle sur IRM que le TDM seul, et dans le cas de l’Hôtel-Dieu, avec une charge clinique moindre que l’IRM. / The present work explores the clinical potential of the adapted prototype of the Clarity AutoScan probe for gynecological brachytherapy use. The goal of this project is to determine whether a 3D transabdominal ultrasound (3DTAUS) can improve treatment planning when combine to computed tomography (CT) compared to CT-based treatment planning. For the purpose of this work, 19 patients were analyzed along the various steps of the study. The two main parts are the contour analysis and the treatment planning analysis. In the first part, images set (magnetic resonance images (MRI), CT and 3DTAUS) of 6 patients were analyzed to determine the visibility of various structures. The comparative high-risk clinical target volume (HR-CTV) contour analysis included 5 patients was performed with 4 images sets (MRI, CT, 3DTAUS and CT-3DTAUS). The HR-CTV volume and lateral dimensions were compared to the reference set, the MRI. Treatment plans optimized on MRI, CT and CT-3DTAUS were elaborated for 5 patients. MRI treatment plans were evaluated on CT and CT-3DTAUS, and CT and CT-3DTAUS were evaluated on MRI. HR-CTV and tandem can be seen on 3DTAUS counter to rectum, sigmoid and ovoids. HRCTV contour analysis shows that 3DTAUS contours, on average, are the ones closer to MRI but with the higher variability. For treatment planning, the treatment plans optimized on CT-3DTAUS are the one closer to MRI, showing improvement to CT plans while combining it to 3DTAUS. For complete 3D treatment planning, 3DTAUS can be used alone. However, the CT-3DTAUS fusion allows treatment plans closer to MRI than CT alone. For the reason, 3DTAUS should be added to the current clinical workflow when MRI is not available.