The biological datasets produced as a result of high-throughput genomic research such as specifically microarrays, contain vast amounts of knowledge for entire genome and their expression affiliations. Gene clustering from such data is a challenging task due to the huge data size and high complexity of the algorithms as well as the visualization needs. Most of the existing analysis methods for genome-wide gene expression profiles are sequential programs using greedy algorithms and require subjective human decision. Recently, Zhu et al. proposed a parallel Random matrix theory (RMT) based approach for generating transcriptional networks, which is much more resistant to high level of noise in the data [9] without human intervention. Nowadays GPUs are designed to be used more efficiently for general purpose computing [1] and are vastly superior to CPUs [6] in terms of threading performance. Our kernel functions running on GPU utilizes the functions from both the libraries of Compute Unified Basic Linear Algebra Subroutines (CUBLAS) and Compute Unified Linear Algebra (CULA) which implements the Linear Algebra Package (LAPACK). Our experiment results show that GPU program can achieve an average speed-up of 2~3 times for some simulated datasets.

Submitted by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-09-08T11:57:20Z No. of bitstreams: 1 KarinaRibeiroModestoDissertacao2017.pdf: 6705375 bytes, checksum: 161944cc6fbeb260f63db5fea9e61056 (MD5) / Approved for entry into archive by Sara Ribeiro (sara.ribeiro@ucb.br) on 2017-09-08T11:57:33Z (GMT) No. of bitstreams: 1 KarinaRibeiroModestoDissertacao2017.pdf: 6705375 bytes, checksum: 161944cc6fbeb260f63db5fea9e61056 (MD5) / Made available in DSpace on 2017-09-08T11:57:33Z (GMT). No. of bitstreams: 1 KarinaRibeiroModestoDissertacao2017.pdf: 6705375 bytes, checksum: 161944cc6fbeb260f63db5fea9e61056 (MD5) Previous issue date: 2017-03-31 / Recent discoveries points towards seminal plasma playing a major role in directing processes on mammalian spermatozoa as well as influencing offspring development prior to and after birth. Amongst the candidates for such role, recent data has brought the extracellular vesicles (EVs) into the field. Aiming to characterize EVs present on the intraluminal fluid of different segments of the epididymis, such as cauda and caput in male Wistar rats, this research assessed the influence of sexual developmental stages on the concentration and size distribution of epididymal EVs. The individuals were also grouped in terms of sexual developmental stages, clustered as ???prior to puberty, ???puberty???, and ???post-puberty???. TRPS based analysis showed a significant shifts in concentration and diameter of such EVs between clustering groups as well as epididymal segment groups. Caput epididymal EVs were found presenting reduced concentration and reduced diameter when compared to cauda epididymis EVs. Regarding the more sexually developed individuals, those showed higher EVs counts than ???prior to puberty??? ones. Additional findings suggest that post-puberty epididymal EVs are enlarged in comparison to the two other groups. Such data might serve a purpose of better comprehending the processes regarding spermatic maturation and capacitation (fertilization), offering findings that bring up clinically important issues regarding male epigenesis and father-to-offspring effects. / Evid??ncias crescentes t??m apontado para a import??ncia do conte??do presente no plasma seminal de mam??feros sobre as c??lulas esperm??ticas bem como sobre o desenvolvimento da prole durante os per??odos pr?? e p??s-natal. Entre os mediadores aparentemente envolvidos nesse processo, podemos citar as ves??culas extracelulares (VEs). Com o objetivo de caracterizar as VEs presentes no l??quido intraluminal de diferentes compartimentos do epid??dimo, cabe??a e cauda, de ratos machos da linhagem Wistar, o presente trabalho avaliou a influ??ncia da idade sobre a concentra????o e a distribui????o de tamanho dessas ves??culas nos compartimentos do epid??dimo. Para isso, utilizou-se tr??s grupos de ratos em idades distintas, sendo um grupo com indiv??duos em idade pr??-p??bere, um grupo com indiv??duos em idade p??bere e um grupo com indiv??duos em idade p??s-p??bere. A contagem de part??culas por detec????o de pulso resistivo ajust??vel (TRPS) revelou diferen??as importantes na concentra????o e no di??metro dessas VEs, tanto em fun????o do segmento anat??mico epididim??rio, quanto em fun????o da idade dos indiv??duos. A cabe??a do epid??dimo apresentou menor concentra????o de part??culas em compara????o com a cauda, bem como part??culas com di??metro menor. Com rela????o a idade, os ratos mais velhos apresentaram maior concentra????o de VEs quando comparados com os ratos imaturos sexualmente. Al??m disso, foi observado que as VEs presentes no ducto epididim??rio dos ratos p??s-p??beres possui tamanho maior que as VEs dos outros dois grupos experimentais. Os resultados aqui obtidos podem servir para uma melhor compreens??o dos processos que englobam tanto a matura????o e capacita????o esperm??tica em si, oferecendo propriedades clinicamente relevantes, quanto processos da espermatog??nese masculina e poss??veis efeitos epigen??ticos transgeracionais.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-12-01T23:35:59Z No. of bitstreams: 1 EllisonMatheusDelioAlcantara_DISSERT.pdf: 1987085 bytes, checksum: 4511f07e0b4aeaea594ec88fad96b400 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-12-05T22:39:58Z (GMT) No. of bitstreams: 1 EllisonMatheusDelioAlcantara_DISSERT.pdf: 1987085 bytes, checksum: 4511f07e0b4aeaea594ec88fad96b400 (MD5) / Made available in DSpace on 2017-12-05T22:39:59Z (GMT). No. of bitstreams: 1 EllisonMatheusDelioAlcantara_DISSERT.pdf: 1987085 bytes, checksum: 4511f07e0b4aeaea594ec88fad96b400 (MD5) Previous issue date: 2017-11-27 / As preocupa??es ambientais v?m aumentando nos ?ltimos anos, principalmente devido ao uso de v?rios produtos derivados de petr?leo, os quais levam anos para se degradar na natureza. Para minimizar esse problema, v?rios estudos focam na utiliza??o de materiais naturais biodegrad?veis. Nesse contexto, verificou-se que a f?cula de mandioca pode ser aproveitada como mat?ria prima para produ??o de um biopol?mero que pode ser utilizado como isolante t?rmico. O biopol?mero foi produzido por um processo de expans?o t?rmica controlada com fra??es volum?tricas de 30% de ?gua e 70% de f?cula de mandioca. Foram analisadas as propriedades f?sicas e t?rmicas do material com intuito de obter dados sobre a viabilidade de seu uso como isolante t?rmico. O material apresentou uma condutividade t?rmica de 0,152 W/mK, podendo ser aplicado como isolante em sistemas quente de at? 270 ?C e um limite de resist?ncia a temperatura de 270?C observado pela an?lise termogravim?trica. O biopol?mero de f?cula de mandioca mostrou-se eficiente quanto ? isola??o t?rmica, devido possuir propriedades semelhantes ?s de isolantes t?rmicos dispon?veis comercialmente. Al?m disso, foram realizadas an?lises de inflamabilidade, a qual o material se apresentou como n?o inflam?vel. Al?m disso, analisou-se dureza caracterizando-o como um material duro. Diante disso, o material mostrou-se promissor a ser aplicado como isolante t?rmico. Essas an?lises foram fundamentadas em normas ABNT, ASTM e UL-94 e os resultados obtidos experimentalmente comprovam que o biopol?mero de f?cula de mandioca pode ser utilizado para fins de isolamento t?rmico. / Environmental concerns have been rising in recent years, mainly due to the use of various petroleum products, which take years to degrade in nature. To minimize this problem, several studies focus on the use of natural biodegradable materials. In this context, it has been found that manioc starch can be used as raw material for the production of a biopolymer that can be used as a thermal insulation. The biopolymer was produced by a controlled thermal expansion process with volumetric fractions of 30% water and 70% cassava starch. The physical and thermal properties of the material were analyzed in order to obtain data on the feasibility of its use as thermal insulation. The material had a thermal conductivity of 0.152 W / mK, and can be applied as an insulator in hot systems up to 270 ? C and a temperature resistance limit of 270 ? C observed by thermogravimetric analysis. Cassava starch biopolymer proved to be efficient in thermal insulation, due to its properties similar to those of commercially available thermal insulators. In addition, flammability analyzes were performed, to which the material presented as non-flammable. In addition, hardness was analyzed by characterizing it as a hard material. In view of this, the material proved promising to be applied as a thermal insulation. These analyzes were based on ABNT, ASTM and UL-94 standards and the results obtained experimentally prove that the biopolymer of cassava starch can be used for thermal insulation purposes.

Made available in DSpace on 2014-12-17T14:07:15Z (GMT). No. of bitstreams: 1 MaraTST_DISSERT.pdf: 2071273 bytes, checksum: 9636b6602dd1a469f78b17d077ba757f (MD5) Previous issue date: 2013-03-04 / In recent decades have seen a sharp growth in the study area of nanoscience and nanotechnology and is included in this area, the study of nanocomposites with self-cleaning properties. Since titanium dioxide (TiO2) has high photocatalytic activity and also antimicrobial, self-cleaning surfaces in your application has been explored. In this study a comparison was made between two synthesis routes to obtain TiO2 nanoparticles by hydrothermal method assisted by microwave. And after analysis of XRD and SEM was considered the best material for use in nanocomposites. It was deposited nanocomposite film of poly (dimethyl siloxane) (PDMS) with 0.5, 1, 1.5 and 2% by weight of nanoparticles of titanium dioxide (TiO2) by the spraying method. The nanocomposite was diluted with hexane and the suspension was deposited onto glass substrate, followed by curing in an oven with forced air circulation. The photocatalytic activity of the nanocomposite impregnated with methylene blue was evaluated by UV- vis spectroscopy from the intensity variation of absorption main peak at 660nm with time of exposure to the UV chamber. Changes in the contact angle and microhardness were analyzed before and after UV aging test. The effect of ultraviolet radiation on the chemical structure of the PDMS matrix was evaluated by spectrophotometry Fourier transform infrared (FTIR).The results indicated that the addition of TiO2 nanoparticles in the coating PDMS gave high photocatalytic activity in the decomposition of methylene blue, an important characteristic for the development of self-cleaning coatings / Nas ?ltimas d?cadas tem-se observado um crescimento acentuado no estudo da ?rea de nanoci?ncia e nanotecnologia em que inclui-se nessa ?rea, o estudo de nanocomp?sitos com propriedades autolimpantes. Desde que o di?xido de tit?nio (TiO2) apresenta alta atividade fotocatal?tica e tamb?m, atividade antimicrobiana, sua aplica??o em superf?cies autolimpantes tem sido amplamente explorada. Neste trabalho foi feito uma compara??o entre duas rotas de s?ntese para obten??o de nanopart?culas de TiO2 pelo m?todo hidrot?rmico assistido por micro-ondas. Ap?s an?lise de DRX e MEV foi analisado o melhor material para aplica??o em nanocomp?sitos. Foram depositados filmes de nanocomp?sito de poli(dimetil siloxano) (PDMS) com 0,5, 1, 1,5 e 2% em massa de nanopart?culas de di?xido de tit?nio (TiO2) pelo m?todo de aspers?o. O nanocomp?sito foi dilu?do em hexano e a suspens?o foi depositada sobre l?minas de vidro, seguida de cura em estufa com circula??o for?ada de ar. A atividade fotocatal?tica do nanocomp?sito impregnado com azul de metileno foi avaliada pela t?cnica de espectroscopia de UV-V?sivel, a partir da varia??o da intensidade de absor??o do pico principal a 660 nm com o tempo de exposi??o em c?mara UV. Altera??es no ?ngulo de contato e na microdureza foram analisadas antes e ap?s o ensaio de envelhecimento UV. O efeito da radia??o ultravioleta na estrutura qu?mica da matriz de PDMS foi avaliado por espectrofotometria no infravermelho por transformada de Fourier (FTIR). Os resultados indicaram que a adi??o das nanopart?culas de TiO2 em PDMS conferiram ao revestimento boa atividade fotocatal?tica na decomposi??o do azul de metileno, caracter?stica importante para o desenvolvimento de revestimentos autolimpantes

Made available in DSpace on 2015-03-03T15:15:28Z (GMT). No. of bitstreams: 1 RodolfoBS_DISSERT.pdf: 2972614 bytes, checksum: 2be8de04b2ac1f09c7c087cfcd17803d (MD5) Previous issue date: 2010-09-10 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Samples of lanthanum Ortoferrites doped with strontium were synthesized in a single phase by the sol-gel method. Two samples were prepared, one by varying the concentration of strontium in lanthanum ortoferrites La1?xSrxFeO3?? with (0 ? x ? 0.5), and another batch of samples of type, La1/3Sr2/3FeO3??, now varying only the temperature of calcination. Our samples were obtained by Pechini method and sintered in air and oxygen atmospheric. Their crystal structures were determined by x-ray diraction (XRD), scanning electron microscopy (SEM), where we observed that the samples (0 ? x ? 0.3) have orthorhombic symmetry and the volume of the single cell decreases with the increasing of concentration of strontium. For x = 0.5 it is only observed the simple phase when that is sintered in O2 atmospheric. Their magnetic characteristics were obtained by the M?ssbauer spectroscopy and magnetic measurements. The magnetization measurements for samples La1?xSrxFeO3?? with (0 ? x ? 0.5) revealed that the magnetization decreases with increasing concentration of strontium, but for the sample x = 0.4 the magnetization shows a high coercive field and a ferrimagnetic behavior, which is attributed to a small amount of strontium hexaferrite. As for the samples La1/3Sr2/3FeO3?? calcined between 800 oC e 1200 oC. The hysteresis curves revealed two distinct behaviors: an declined antiferromagnetic behavior (Canted) for samples calcined between 800 oC and 1000 oC and a paramagnetic behavior for the samples calcined at 1100 oC e 1200 o C. Thermal hysteresis and sharp peaks around the N?el temperature (TN), over the curves of specific heat as a function of temperature was only observed in calcined samples with 1100 oC and 1200 oC. This eect is attributed to the charge ordering. These results indicate that the charge ordering occurs only in the samples without oxygen deficiency. Magnetic measurements as a function of temperature are also in agreement with this interpretation / ?Amostras de ortoferritas de lant?nio dopados com estr?ncio foram sintetizados na forma de fase simples pelo m?todo Sol-Gel. Dois lotes de amostras foram preparadas, um variando a concentra??o do estr?ncio nas ortoferritas de lant?nio La1?xSrxFeO3?? com (0 ? x ? 0.5), e um outro lote de amostras do tipo La1/3Sr2/3FeO3??, agora variando somente a temperatura de calcina??o. Nossas amostras foram obtidas pelo m?todo Pechini, e sinterizados em atmosfera de oxig?nio. Suas estruturas cristalinas foram determinadas por difratometria de raios -x (DRX), microscopia eletr?nica de varredura (MEV), onde observamos que as amostras (0 ? x ? 0.3) possuem simetria ortorr?mbica, e o volume da c?lula unit?ria diminui com o aumento da concentra??o de estr?ncio. Para x=0.5 somente ? observada fase simples quando esta ? sinterizada na atmosfera de O2. Suas caracter?sticas magn?ticas foram obtidas por meio de espectroscopia M?ossbauer e medidas de magnetiza??o. As medidas de magnetiza??o para as amostras La1?xSrxFeO3?? com (0 ? x ? 0.5) revelaram que a magnetiza ??o decresce com o aumento da concentra??o de estr?ncio, contudo para a amostra x=0.4 a magnetiza??o mostra um alto campo coercivo e um comportamento ferrimagn?tico, o qual ? atribuido a uma pequana quantidade de Hexaferrita de estr?ncio. J? para as amostras La1/3Sr2/3FeO3??, calcinadas entre 800 oC e 1200 oC , as curvas de histerese revelaram dois comportamentos distintos: Um comportamento antiferromagn?tico declinado (Canted) para as amostras calcinadas entre 800 oC e 1000 oC e um comportamento paramagn?tico para as amostras calcinadas em 1100 oC e 1200 o C. Histereses t?rmicas e picos acentuados em torno da temperatura de N?el (TN), sobre as curvas de calor espec? co em fun??o da temperatura foi observada apenas nas amostras calcinadas com 1100 oC e 1200 oC . Este efeito ? atribuido ao ordenameto de carga. Estes resultados indicam que o ordenamento de carga ocorre somente nas amostras sem de ci?ncia de oxig?nio. Medidas de Magnetiza??o em fun??o da temperatura tamb?m est?o de acordo com esta interpreta??o.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2018-03-12T13:04:19Z No. of bitstreams: 1 RafaelChavesLima_DISSERT.pdf: 15500276 bytes, checksum: 4f1a759afd6ed9c7eeb174d02f229d3e (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-03-13T20:23:09Z (GMT) No. of bitstreams: 1 RafaelChavesLima_DISSERT.pdf: 15500276 bytes, checksum: 4f1a759afd6ed9c7eeb174d02f229d3e (MD5) / Made available in DSpace on 2018-03-13T20:23:09Z (GMT). No. of bitstreams: 1 RafaelChavesLima_DISSERT.pdf: 15500276 bytes, checksum: 4f1a759afd6ed9c7eeb174d02f229d3e (MD5) Previous issue date: 2018-01-30 / O Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / A assimila??o do modo como uma ze?lita ? formada partindo de um sistema amorfo e o controle sobre tamanho e morfologia dos produtos de s?ntese s?o aspectos fundamentais para a ?engenharia zeol?tica?. Um sistema para a s?ntese da ze?lita mordenita foi estudado, o qual possibilitou a obten??o de fase MOR sem indica??o da presen?a de qualquer outra fase. Foram realizadas varia??es nos par?metros: condi??o de tratamento t?rmico, temperatura, tempo, dilui??o do gel, teor e grau de pureza da ?gua, teor de s?dio, envelhecimento e tipo de s?lica, visando o estabelecimento da rela??o entre o tamanho e a morfologia dos produtos de s?ntese com as modifica??es efetuadas. A temperatura de cristaliza??o mostrou ser um fator primordial com rela??o ao tamanho de part?culas e cristalitos. A dilui??o do gel de s?ntese (50% m/m) proporcionou uma redu??o na agrega??o dos cristalitos e a varia??o do teor de ?gua na s?ntese implicou em mudan?as nas taxas de zeolitiza??o. O teor de s?dio modificou o crescimento dos cristais e o aumento de intercrescimentos e agrega??es de part?culas. Mudan?as na fonte de s?lica incrementaram a taxa de crescimento dos cristais e contribuiram para altera??es no tamanho dos mesmos. Cristais maiores foram obtidos a partir de sistemas com maior concentra??o de c?tions s?dio enquanto agregados de nanocristalitos. Agregados de pequenos cristalitos de mordenita pura de at? cerca de 50 nm foram obtidos com s?nteses realizadas a 150 ?C e com a dilui??o do gel j? preparado. Menores teores de s?dio e ?gua e o uso de fontes de s?lica de maior ?rea espec?fica (relativa a este tipo de material) tamb?m tendem ? resultar em menores cristalitos. Condi??es opostas viabilizaram o maior crescimento, gerando cristais de at? 1 ?m em m?dia. Os resultados mostraram que ? poss?vel exercer algum controle sobre o tamanho de part?cula e de cristalitos da ze?lita mordenita atrav?s do ajuste de par?metros inerentes ao sistema convencional de s?ntese. / The assimilation of the way a zeolite is formed from an amorphous system and the control over size and morphology of the synthesis products are fundamental aspects for "zeolitic engineering". A system for the synthesis of the mordenite zeolite was studied, which made it possible to obtain MOR phase without indicating the presence of any other phase. Variations were made in the parameters: thermal treatment condition, temperature, time, gel dilution, water content and degree of purity, sodium content, aging and type of silica, aiming to establish the relationship between product size and morphology with the modifications made. The crystallization temperature showed to be a prime factor with respect to particle size and crystallites. Dilution of the synthesis gel (50% w / w) provided a reduction in the crystallite aggregation and the variation of the water content in the synthesis implied changes in zeolitization rates. The sodium content modified the growth of the crystals and the increase of intergrowths and aggregations of particles. Changes in the silica source increased the growth rate of the crystals and contributed to changes in their size. Larger crystals were obtained from systems with higher concentrations of sodium cations as nanocrystallite aggregates. Aggregates of small pure mordenite crystallites of up to about 50 nm were obtained with syntheses performed at 150 ? C and with the dilution of the already prepared gel. Lower sodium and water content and the use of silica sources with a greater specific area (relative to this type of material) also tend to result in smaller crystallites. Opposite conditions enabled the highest growth, generating crystals up to 1 ?m on average. The results showed that it is possible to exert some control over the particle size and crystallites of the mordenite zeolite by adjusting parameters inherent to the conventional synthesis system.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-06-02T23:35:26Z No. of bitstreams: 1 JonasBispoPessoa_DISSERT.pdf: 2858666 bytes, checksum: e23bdc728a23ba1b10c5f54490f0ed53 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-06-12T22:35:05Z (GMT) No. of bitstreams: 1 JonasBispoPessoa_DISSERT.pdf: 2858666 bytes, checksum: e23bdc728a23ba1b10c5f54490f0ed53 (MD5) / Made available in DSpace on 2017-06-12T22:35:05Z (GMT). No. of bitstreams: 1 JonasBispoPessoa_DISSERT.pdf: 2858666 bytes, checksum: e23bdc728a23ba1b10c5f54490f0ed53 (MD5) Previous issue date: 2016-12-06 / As nanopart?culas de ouro (NPO) t?m despontado como uma importante modalidade de tratamento para o c?ncer, devido ?s suas caracter?sticas f?sico-qu?micas. Somado a isso, o anti-hipertensivo carvedilol tem demonstrado, na literatura recente, potencial antitumoral. Diante disto, o objetivo do trabalho foi avaliar a a??o antitumoral, isolada e combinada, da nanopart?cula de ouro e do anti-hipertensivo carvedilol sobre c?lulas tumorais (HepG2) e n?o tumorais (HEK-293) nos tempos de 24 horas e 48 horas. Para tanto, foi realizado o teste de viabilidade pela exclus?o do azul tripan para as doses de NPO (1 ?g/ml, 3 ?g/ml, 6,25 ?g/ml, 12,5 ?g/ml, 25 ?g/ml e 50?g/ml) e carvedilol (1,5 ?M, 3 ?M, 6,25 ?M, 12,5 ?M, 50 ?M, 100 ?M, 200 ?M e 300 ?M) para selecionar as que provocassem baixa inibi??o do crescimento das c?lulas da linhagem HepG2. No tratamento combinado, as c?lulas foram submetidas ao tratamento com a NPO e, 24 horas depois, tratadas com carvedilol em diferentes doses (3 ?g/ml de NPO + 1,5 ?M de carvedilol e 6,25 ?g/ml de NPO + 3 ?M de carvedilol). As doses selecionadas do teste de viabilidade da NPO (3 ?g/ml e 6,25 ?g/ml) e carvedilol (1,5 ?M e 3 ?M) foram utilizadas, isoladas e em combina??o, para an?lise de morte celular por citometria de fluxo pela marca??o da Anexins V-FITC e iodeto de prop?dio (PI). A an?lise de estresse oxidativo foi realizada atrav?s da dosagem de GSH e dos n?veis de Malondialde?do (MDA). Posteriormente, as c?lulas tumorais foram analisadas quanto a express?o das prote?nas caspase-3, caspase-8, Bcl-2 e MAPK/ERK atrav?s da microscopia de imunofluoresc?ncia. Em seguida, os n?veis de mRNA de FADD, Apaf-1, survivin, MDR-1, EGFR, Akt e mTOR foram analisados relacionando-os a resist?ncia e morte celular. Os n?veis proteicos de Akt, mTOR e MAPK, para o tratamento combinado, foram mensurados atrav?s do western blotting. A avalia??o dos alvos intracelulares da NPO, isolada e em combina??o, foi realizada atrav?s da microscopia eletr?nica de transmiss?o (MET). As melhores doses do teste de viabilidade celular com a NPO (3 ?g/ml e 6,25 ?g/ml) e carvedilol (1,5 ?M e 3 ?M) apresentaram, atrav?s da citometria de fluxo, atividade pr?-apopt?tica sobre as c?lulas tumorais humanas (HepG2) com resultados estaticamente significativos para a combina??o (P<0,001). Em rela??o ?s c?lulas n?o tumorais (HEK-293), houve redu??o da apoptose total (P<0,05), para a maior dose da combina??o, e redu??o do estresse oxidativo na qual, os n?veis de GSH, mostrou-se elevado (P=0,0003) e os de MDA reduzidos (P<0,01), demonstrando capacidade protetora. Ao se observar a imunofluoresc?ncia, houve forte marca??o para caspase-3 (P<0,01) e caspase-8 (P<0,001), nos grupos tratados com a NPO (6,25 ?g/ml) e carvedilol (3?M), isoladamente e em combina??o, aus?ncia de marca??o de MAPK/ERK (P<0,001) e manuten??o da express?o de Bcl-2 (P>0,05) para os mesmos grupos. Al?m disso, a express?o de mRNA de prote?nas anti-apopt?ticas (EGFR Akt, mTOR), com P<0,001, e de resist?ncia (MDR-1), com P<0,01, mostrou-se subregulado, enquanto que, a express?o g?nica de prote?nas proapopt?ticas (FADD), com P<0,01, apresentou-se sobre-regulado para o tempo de 48 horas para combina??o de 6,25 ?g/ml de NPO e 3 ?M de carvedilol. Al?m disso, os n?veis proteico de Akt, mTOR e MAPK se encontraram reduzidos. A MET demonstrou a internaliza??o da NPO isolada, nas proximidades da membrana plasm?tica e, em combina??o, nas proximidades do n?cleo Com isso, pode-se concluir que a a??o combinada da NPO e carvedilol mostra efeitos proapopt?ticos sobre c?lulas tumorais mais efetivos que as doses isoladas e prote??o de c?lulas n?o tumorais. / Gold nanoparticles (NPO) have emerged as an important modality of treatment for cancer, due to their physicochemical characteristics. In addition to this, the antihypertensive carvedilol has been shown in the recent literature to be antitumor potential. Therefore, the objective of this study was to evaluate the antitumor action, isolated and combined, of the gold nanoparticle and antihypertensive carvedilol on tumor cells (HepG2) and non-tumor cells (HEK-293) at 24 hours and 48 hours. The viability test was carried out by exclusion of tripan blue at doses of NPO (1 ?g / ml, 3 ?g / ml, 6.25 ?g / ml, 12.5 ?g / ml, 25 ?g / ml and 50 ?g / Ml) and carvedilol (1.5 ?M, 3 ?M, 6.25 ?M, 12.5 ?M, 50 ?M, 100 ?M, 200 ?M and 300 ?M) were used to select those that would cause low inhibition of growth of HepG2 cells . In the combined treatment, cells were treated with NPO and 24 hours later treated with carvedilol at different doses (3 ?g / ml NPO + 1.5 ?M carvedilol and 6.25 ?g / ml NPO + 3 ?M carvedilol). Selected doses of NPO viability test (3 ?g / ml and 6.25 ?g / ml) and carvedilol (1.5 ?M and 3 ?M) were used, alone and in combination, for cell death analysis by flow cytometry By the labeling of Anexins V-FITC and propidium iodide (PI). Oxidative stress analysis was performed by the determination of GSH and the levels of Malondialdehyde (MDA). Subsequently, tumor cells were analyzed for expression of caspase-3, caspase-8, Bcl-2 and MAPK / ERK proteins by immunofluorescence microscopy. Then, mRNA levels of FADD, Apaf-1, survivin, MDR-1, EGFR, Akt and mTOR were analyzed by relating them to resistance and cell death. Protein levels of Akt, mTOR and MAPK, for the combined treatment, were measured by western blotting. The evaluation of the intracellular targets of NPO, isolated and in combination, was performed by transmission electron microscopy (MET). The best doses of the cell viability test with NPO (3 ?g / ml and 6.25 ?g / ml) and carvedilol (1.5 ?M and 3 ?M) showed, by flow cytometry, pro-apoptotic activity on cells (HepG2) with statically significant results for the combination (P <0.001). In relation to the non-tumor cells (HEK-293), there was a reduction of total apoptosis (P <0.05), for the highest dose of the combination, and reduction of oxidative stress in which GSH levels were elevated P = 0.0003) and those of reduced MDA (P <0.01), showing protective capacity. When the immunofluorescence was observed, there were strong markers for caspase-3 (P <0.01) and caspase-8 (P <0.001) in the groups treated with NPO (6.25 ?g / ml) and carvedilol (3 ?M) Isolated and in combination, absence of MAPK / ERK labeling (P <0.001) and maintenance of Bcl-2 expression (P> 0.05) for the same groups. In addition, the expression of mRNA of anti-apoptotic proteins (EGFR Akt, mTOR), with P <0.001, and resistance (MDR-1), with P <0.01, was shown to be underregulated, whereas expression (PADF), with P <0.01, was overregulated for 48 hours for the combination of 6.25 ?g / ml NPO and 3 ?M carvedilol. In addition, the protein levels of Akt, mTOR and MAPK were reduced. The MET demonstrated the internalization of NPO alone, in the vicinity of the plasma membrane and, in combination, in the vicinity of the nucleus. Thus, it can be concluded that the combined action of NPO and carvedilol shows proapoptotic effects on tumor cells more effective than isolated doses And protection of non-tumor cells.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-10-03T00:21:48Z No. of bitstreams: 1 MayraKerollySalesMonteiro_DISSERT.pdf: 5097719 bytes, checksum: 8a31b93e5438ec081f424997340a4eb4 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-10-09T22:11:42Z (GMT) No. of bitstreams: 1 MayraKerollySalesMonteiro_DISSERT.pdf: 5097719 bytes, checksum: 8a31b93e5438ec081f424997340a4eb4 (MD5) / Made available in DSpace on 2017-10-09T22:11:42Z (GMT). No. of bitstreams: 1 MayraKerollySalesMonteiro_DISSERT.pdf: 5097719 bytes, checksum: 8a31b93e5438ec081f424997340a4eb4 (MD5) Previous issue date: 2017-07-28 / Biofilmes e revestimentos comest?veis s?o camadas finas de materiais biodegrad?veis aplicados sobre produtos alimentares que desempenham um papel importante na sua preserva??o, distribui??o e comercializa??o. A f?cula de mandioca ? um dos melhores candidatos ? prepara??o de revestimentos comest?veis, por ser um pol?mero completamente biodegrad?vel no solo e na ?gua e de f?cil obten??o. No entanto, a f?cula possui limita??es em suas propriedades f?sico-qu?micas quando comparada aos pol?meros sint?ticos. Por sua vez, o melhoramento destas propriedades foi proposto neste estudo a partir da adi??o de 5%, em massa, de argila em rela??o ? massa seca de biopol?mero. A compatibiliza??o superficial da argila com a matriz biopolim?rica foi realizada por modifica??o superficial na presen?a de tr?s tipos de tensoativos (brometo de cetiltrimetil am?nio, dodecil sulfato de s?dio e ?lcool laur?lico etoxilado). O efeito de tr?s modifica??es sequenciais e cumulativas na argila foi caracterizado por Difra??o de Raios X (DRX), Transformada de Fourier por Radia??o Infravermelha (FTIR), Microscopia Eletr?nica de Varredura (MEV), Espectroscopia de Energia Dispersiva de Raios X (EDS), ?ngulo de Contato (AC), Potencial Zeta (ZETA) e An?lise Termogravim?trica (TGA). A argila modificada sinergicamente na presenta dos tr?s tensoativos evidenciou o maior distanciamento basal entre as camadas de silicato, cerca de 10 nm a mais em rela??o ? argila natural. Os filmes de f?cula de mandioca com argila natural e modificada foram caracterizados como bionanocomp?sito intercalado ou esfoliado atrav?s das an?lises de Microscopia de For?a At?mica (AFM), Microscopia ?ptica (MO), DRX, FTIR, MEV e AC. A influ?ncia da argila modificada e da natural na mudan?a das propriedades f?sico-qu?micas dos filmes de f?cula original foi investigada quanto ? permeabilidade ao vapor d??gua, ductibilidade, opacidade, estabilidade t?rmica e solubilidade em ?gua. Por fim, a argila modificada sinergicamente na presenta dos tr?s tensoativos foi o material de refor?o que mais contribuiu para o melhoramento das propriedades f?sico-qu?micas do filme de f?cula controle, dentre as quais reduziu 90,6% da permeabilidade ao vapor d??gua, bem como 77,43% da dissolu??o m?xima. / Biofilms and edible coatings are thin layers of biodegradable materials applied to food products that play an important role in their preservation, distribution and commercialization. Cassava starch is one of the best candidates for the preparation of edible coatings, because it is a completely biodegradable polymer in soil and water and easy to obtain. However, the starch has limitations in its physical-chemical properties when compared to the synthetic polymers. In turn, the improvement of these properties was proposed in this research from the addition of 5%, by mass, of clay in relation to the dry mass of biopolymer. The surface compatibilization of the clay with the biopolymer matrix was performed by surface modification in the presence of three types of surfactants (cetyltrimethyl ammonium bromide, sodium dodecyl sulfate and ethoxylated lauryl alcohol). The effect of three sequential modifications on the clay was characterized by X-ray Diffraction (XRD), Fourier Transform by Infrared Radiation (FTIR), Scanning Electron Microscopy (SEM), Dispersive Lightning Spectroscopy (DLS), Contact Angle (AC), Zeta Potential (ZETA) and Thermogravimetric Analysis (TGA). The clay modified synergistically in the presence of the three surfactants showed the greatest basal space between the silicate layers, about 10 nm more than the natural clay. The cassava starch films with natural and modified clay were characterized as bionanocomposite intercalated or exfoliated through Atomic Force Microscopy (AFM), Optical Microscopy (MO), XRD, FTIR, SEM and AC analyzes. The influence of modified and natural clay on the change in the physical-chemical properties of the original starch films was investigated for water vapor permeability, ductility, opacity, thermal stability and water solubility. Finally, the synergistically modified clay in the presence of the three surfactants was the reinforcing material that contributed the most to the physical-chemical properties of the control starch film, among which it reduced the permeability to water vapor by 90.6%, as well as 77.43% of the maximum dissolution.

Submitted by Verena Pereira (verenagoncalves@uefs.br) on 2018-07-11T23:13:30Z No. of bitstreams: 1 DIsserta??o Alexsandro VFA HIST?RIA DO M?SON PI .pdf: 9797951 bytes, checksum: 05cccd1dc1df86aff95c514b9ac8f4ab (MD5) / Made available in DSpace on 2018-07-11T23:13:30Z (GMT). No. of bitstreams: 1 DIsserta??o Alexsandro VFA HIST?RIA DO M?SON PI .pdf: 9797951 bytes, checksum: 05cccd1dc1df86aff95c514b9ac8f4ab (MD5) Previous issue date: 2017-08-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This work represents our contribution to disseminate Brazilian Physics in the classroom. It consists of a qualitative research about the insertion of topics of Particle Physics through a conceptual approach, particularly the first proposal of Yukawa for strong interaction, from the history of the discovery of the ? meson with the contributions of the brazilian scientist, C?sar Lattes. The research consisted in the elaboration, application and evaluation of a didactic sequence based on the theory of significant learning of David Ausubel / Com este estudo, pretende-se contribuir para a divulga??o da F?sica brasileira em sala de aula. Ele consiste em uma pesquisa qualitativa que trata da inser??o de t?picos de F?sica de Part?culas atrav?s de abordagem conceitual, particularmente a primeira proposta de Yukawa para intera??o forte, a partir da hist?ria da descoberta do m?son ? com as contribui??es do brasileiro C?sar Lattes. A pesquisa consistiu na elabora??o, aplica??o e avalia??o de uma sequ?ncia did?tica fundamentada na teoria de aprendizagem significativa de David Ausubel

Made available in DSpace on 2016-04-28T14:59:00Z (GMT). No. of bitstreams: 1 2009 - Ariane Castricini.pdf: 2010336 bytes, checksum: 05cebf92245d2829733f92cab21a0901 (MD5) Previous issue date: 2009-02-16 / Funda??o Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / This study was conducted in UFRRJ (physico, chemical and microbiological analysis) and Embrapa Agroind?stria de Alimentos (sensory evaluation), with the general objective of assessing the influence of modified atmosphere by coating of starch (cassava starch and modified starch) on the ripening and conservation of papayas (Carica papaya L.) Golden , stored in refrigerated environment. The coating were used in concentrations of 1%, 3% and 5%. The concentrations of cassava starch to 3 and 5% provided better results as ripening: it s delayed the development of color yellow skin but without total development of yellow color and the fruits remained firm for a longer time, during the experimental period. The respiratory rates were lower as higher as the concentration of the coating. The coating of 1% was sufficient to reduce the respiratory rate of fruits, but not positively influenced by other variables. The coatings favored greater integrity of cell walls, demonstrated the proper structure and organization of the same view as the middle lamella. Coatings of modified starch were not effective in preventing ripeness. It did not cause distinct changes in titratable acidity, the content of ascorbic acid and total soluble solids. The results were similar those of uncoated fruits. The respiratory rates were lower in fruits coated film for the 3 and 5%, but not enough to slow the ripening process during storage. The microscopic visualization of cell walls showed good integrity in fruits coated film by the 1 and 5% and evidence of destruction when coated to 3%. The coatings of cassava starch and modified starch affect more the attributes of appearance that the taste of fruits. The coating of cassava starch at 5% was suitable for coating fruits, whereas the majority of the attributes of appearance studied. However, the stripping evidenced by the lower integrity of it, was considered unfavorable. The coating of modified starch and cassava starch at 1% formed intact film, without peeling. The taste was somewhat influenced by the coatings, but the coating of modified starch and 5% changed the characteristic flavor of papaya, evidenced by the bitter taste perceived at 12 and 14 days of storage. The coating of cassava starch with and without sanitary treatment plant favored the development of post-harvest decay, for the film of modified starch. But the largest areas affected by the lesions were observed only in fruits coated by concentrations of 3 and 5% of cassava starch after sanitary treatment plant. The oil inhibited the growth of Colletotrichum gloeosporioides in vitro, but when added to the coatings were not effective in controlling decay. For the sake of strong smell of garlic and cinnamon bark oils left in the fruits, beyond stains caused by concentrations of 1 and 0.5% oil of cinnamon, these products were improper to post-harvest use. / O presente trabalho foi conduzido na UFRRJ (avalia??es f?sicas, qu?micas e microbiol?gicas) e na Embrapa Agroind?stria de Alimentos (an?lise sensorial), com o objetivo geral de avaliar a influ?ncia de atmosfera modificada por revestimentos de amido (f?cula de mandioca e amido modificado) sobre o amadurecimento e conserva??o de mam?es (Carica papaya L.) Golden , armazenados em ambiente refrigerado. Os revestimentos foram utilizados nas concentra??es de 1%, 3% e 5%. As concentra??es de f?cula de mandioca a 3 e 5% proporcionaram melhores resultados, quanto ao amadurecimento. Retardaram o desenvolvimento da colora??o amarela da casca, por?m n?o permitiu o desenvolvimento total da mesma e mantiveram os frutos firmes por mais tempo, durante o per?odo experimental. As taxas respirat?rias foram menores quanto maior a concentra??o dos revestimentos. O revestimento a 1% foi suficiente para reduzir as taxas respirat?rias dos frutos, mas n?o influenciou positivamente nas demais vari?veis estudadas. Os revestimentos favoreceram maior integridade das paredes celulares, evidenciada pela boa estrutura??o e organiza??o das mesmas assim como visualiza??o da lamela m?dia. J? aqueles de amido modificado n?o foram eficientes na preven??o do amadurecimento. Os resultados de acidez total titul?vel, teor de ?cido asc?rbico e s?lidos sol?veis totais foram semelhantes aqueles dos frutos n?o revestidos. As taxas respirat?rias foram menores nos frutos revestidos pelas pel?culas a 3 e 5%, mas n?o o suficiente para desacelerar o processo de amadurecimento, durante o armazenamento. A visualiza??o microsc?pica das paredes celulares revelaram boa integridade nos frutos com pel?culas revestimentos a 1 e 5% e evidenciou desestrutura??o quando revestidos a 3%. Os revestimentos de f?cula de mandioca e amido modificado afetaram mais os atributos de apar?ncia que o sabor dos frutos. A pel?cula de f?cula de mandioca a 5% foi mais adequada para o revestimento dos frutos, considerando a maioria dos atributos de apar?ncia estudados. Por?m, o descascamento evidenciado pela menor integridade da mesma, foi considerado desfavor?vel. O revestimento por amido modificado e f?cula de mandioca a 1% formaram pel?culas ?ntegras, ou seja, sem descascamento. O sabor foi pouco influenciado pelos revestimentos, mas o revestimento de amido modificado a 5% alterou o sabor caracter?stico de mam?o, evidenciado pelo gosto amargo percebido aos 12 e 14? dias de armazenamento. Os revestimentos de f?cula de mandioca com e sem tratamento fitossanit?rio favoreceram o desenvolvimento das podrid?es p?s-colheita, em rela??o ?s pel?culas de amido modificado. Por?m as maiores ?reas afetadas pelas les?es foi observada somente nos frutos revestidos pelas concentra??es de 3 e 5% de f?cula de mandioca ap?s o tratamento fitossanit?rio. Embora os ?leos tenham inibido o crescimento de Colletotrichum gloeosporioides in vitro , n?o foram eficientes no controle quando adicionados aos revestimentos. Devido ao cheiro forte do ?leo de bulbilho de alho e casca de canela deixaram no fruto, al?m das manchas do ?leo de canela, tornam-se esses produtos inadequados para uso em p?s-colheita.