No description available.

An inter-specific linkage map of a backcross (BC1) population derived from a cross between C. tinctorius and C. oxyacanthus was developed using primarily microsatellite markers. A composite map, which aligned the inter-specific (C. tinctorius x C. oxyacanthus) map with an intra-specific linkage map previously developed from an F2 population of C. tinctorius, was created to search for syntenic regions. Results indicate that despite low marker saturation, there is substantial colinearity between the two linkage maps, and one translocation or inversion event. Upon subsequent self-fertilization events, phenotypes of the inter-specific backcross population were characterized in both the field (BC1S2 generation) and growth chamber (BC1S2 and BC1S3 generations), and identified several lines of agronomic interest. Introgression analysis was performed (BC1S3 generation) to assess the level of integration of C. oxyacanthus DNA into the C. tinctorius genome, and results are suggestive of less inter-specific recombination than expected. / Plant Biology

No description available.

Carthamus (Asteraceae) contains both crop species (C. tinctorius, safflower) and weedy species, increasing the need for a better understanding of the genus. Despite previous studies, many outstanding questions remain regarding the phylogenetic relationships of safflower, especially with regards to the weedy species. Investigation of the relationships in Carthamus was done using sequence data. The closest relative to C. tinctorius was studied using microsatellite data. Microsatellite data was also utilized to track the introgression of C. oxyacanthus DNA into the C. tinctorius genome in an interspecific cross. Sequence data supports the division of the genus into two sections, Carthamus and Atractylis. Both sequence and microsatellite data reveal that most traditionally recognized species are not monophyletic. Microsatellite data indicates that C. palaestinus is the closest relative of cultivated safflower. Microsatellites also indicate that C. oxyacanthus DNA is able to move into the C. tinctorius genome, showing potential for breeding programs and raising concerns for potential transgenic crops. / Plant Biology

Submitted by Edineia Teixeira (edineia.teixeira@unioeste.br) on 2018-03-07T18:25:46Z No. of bitstreams: 2 Christian_Oliveira2017.pdf: 823449 bytes, checksum: 8ee30ff0a60f8edcdf0d389856be74ee (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-03-07T18:25:46Z (GMT). No. of bitstreams: 2 Christian_Oliveira2017.pdf: 823449 bytes, checksum: 8ee30ff0a60f8edcdf0d389856be74ee (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2016-11-18 / The society turns its eyes to the future and what seems to be pleseant. Global warming and pollution are recurring issues in all meetings of international summits, because the world is seeking a new way to get their main energy demands and / or natural resources. The exchange of the current energy matrix will not be made in one day, the initiatives has been showing gradually, photovoltaic panels, wind turbines and biofuels seem to wave to a less catastrophic future. However, it is necessary to develop, seek for new sources and crops that have higher efficiency, lower cycle, higher yield and other characteristics. To this end, sometimes the History has shown that in order to find solutions for the future we need to look to the past. So millenarian crops has been proven effective nowadays. One of these crops is Safflower (Carthamus tinctorius L), a millenary plant that used to be an oil and pigment source and which will now be adopted as an object of study. Within this context, this work is organized in two research fronts and the first one is to evaluate the effectiveness of a safflower byproduct and its use in animal feed, and the second one is to evaluate the effectiveness of safflower oil as a possible source of bio-fuel by the transesterification method. The expected contribution is the seal of safflower use and its by-products as candidates for agricultural frontier expansion in Brazil's energy oil production. The intent is to alter the concentration of the traditional diet with addition of safflower meal, which is obtained by cold extrusion grain of 0.5, 10, 15 and 20% order, with a concentration in each sample triplicated in a total of 15 samples. This first step is to analyze the effectiveness of animal growth with the addition of this element in the standard feed. After this first check, there is a new verification phase from the oil generated by the extrusion of safflower. This step is to evaluate the safflower oil as a possible source for the production of bio-fuel by the transesterification method. Analysis of factors, such as viscosity, will be made, calorific value, acid value, free fatty acids and peroxides indexes fall on standards set by the National Petroleum Agency. Understanding it if it is a candidate for the agricultural frontier expansion in Brazil's energy production of oil. / A sociedade volta seus olhos para o futuro e o que vê parece não estar agradando. Aquecimento global e poluição são assuntos recorrentes em todas reuniões de cúpulas internacionais, pois o mundo está buscando uma nova maneira de obter suas principais demandas energéticas e/ou de recursos naturais. A troca da matriz energética atual não vai ser feita instantâniamente, as iniciativas tem se mostrado graduais, painéis fotovoltaicos, aerogeradores e biocombustíveis parecem acenar para um futuro menos catastrófico. Porem, é necessário evoluir, buscar novas fontes, cultivos que tenham maior eficiência, menor ciclo, maior rendimento dentre outras características. Assim, cultivos milenares tem se mostrado eficientes para a solução de problemas atuais. Um destes cultivos é o Cártamo (Carthamus tinctorius L), planta milenar que foi fonte de óleo e pigmentos na antiguidade e que será adotado como objeto neste estudo contemporâneo. Dentro deste contexto o presente trabalho organiza-se em duas frentes de pesquisa sendo que a primeira tem por objetivo avaliar a eficácia de um do sub-produto do cártamo no uso em nutrição animal e a segunda busca avaliar a eficácia do óleo de cártamo como possível fonte de bio-combustível pelo método da transesterificação. A contribuição esperada é a chancela do uso do cártamo e seus subprodutos como candidato à expansão da fronteira agrícola energética brasileira na produção de oleaginosas. O objetivo é alterar a concentração da ração tradicional com adição de farelo de cártamo, que será obtido através da extrusão a frio do grão na ordem de 0, 5; 10; 15 e 20 %, com uma concentração triplicada em cada amostra perfazendo um total de 15 amostras. Encontrando resultados satisfatórios obtidos por inferência estatística de 2,1% de farelo na ração convencional. Após esta primeira verificação o estudo parte para uma nova fase de verificação à partir do óleo gerado pela extrusão do cártamo. Esta etapa tem por objetivo avaliar o óleo de cártamo como possível fonte na produção de bio-combustível pelo método da transesterificação, feita a análise de fatores como viscosidade, poder calorifico, índice de acidez, ácidos graxos livre e índices de peróxidos encontrou-se nesses aspectos padrões enquadrados nos estabelecidos pela Agência Nacional do Petróleo. Entendendo com isso se esta é uma candidata para a expansão da fronteira agrícola energética brasileira na produção de oleaginosas.

No description available.

La industria Argentina ha mostrado un creciente interés en descascarar los frutos de cártamo previo al proceso de extracción de aceite. El descascarado de los frutos permite reducir el volumen de material que circula por el sistema de extracción, el contenido de ceras y pigmentos en el aceite y el de fibra de la harina. La facilidad con que la cáscara se separa de la pepa es un parámetro que puede evaluarse determinando la aptitud al descascarado (AD) de los frutos durante su industrialización. Hasta el momento no se ha evaluado si existe variabilidad genética en este parámetro, ni tampoco cómo podrían afectarlo las modificaciones en el manejo del cultivo. Los objetivos de esta tesis fueron evaluar durante dos años consecutivos: 1) la AD de diferentes variedades de cártamo cultivadas en Argentina, 2) la calidad de la harina de cártamo obtenida de frutos descascarados y sin descascarar, 3) las características morfológicas de los frutos y anatómicas del pericarpio de estas variedades y 4) el efecto de distintas densidades de siembra y distancias entre surcos en el cultivo sobre la AD de los frutos. El ensayo de variedades se realizó en la Estación Experimental Agropecuaria del INTA Hilario Ascasubi (Lat. 39º 23’S; 62° 37’ O) e incluyó siete genotipos de cártamo: Agrosearch L7-3, AG Phoenix, CW 99 OL, Arizona L7-5, Knowles 93079, L-8, Seedtech S- 345. En el mismo sitio experimental, se evaluó el efecto de la densidad de siembra (baja B: 7,5 kg.ha-1 y alta A: 22,5 kg.ha-1) y la distancia entre surcos (35 cm; 52 cm y 70 cm) sobre la AD de los frutos de CW 99 OL. La AD de todas las variedades fue entre un 15 a 46% superior en el primer ciclo de crecimiento (CC) respecto al segundo (p<0,01). Dicho parámetro fue similar entre variedades (68 a 76% en el primer CC y entre 58 a 60% en el segundo), excepto en L-8 en el primer CC y L-8 y Agrosearch L7-3 en el segundo CC (p<0,01). En todas las variedades, el porcentaje de CEM fue de un 20 a 25% en el primer CC y de un 16 a 21% en el segundo CC. La humedad de los frutos al momento de realizarse el descascarado (5%) fue similar entre CC (p>0,05), pero la producción de material fino se redujo a la mitad en el segundo CC (7% y 10,6%) con respecto al primer CC (17% a 23%) en todas las variedades (p<0,01). El descascarado incrementó el contenido de proteína (PB) en la harina de todas las variedades, de 26% a 50% el primer CC y de un 28% a 40% en el segundo (p<0,01). A su vez, disminuyó el contenido de fibra (FDN) de 40,9% a 31,3% (p<0,01) y de dos de sus componentes (celulosa y hemicelulosa; p<0,05), permaneciendo invariable el contenido de lignina (LDA; p>0,05). Estos resultados indican que durante el descascarado de los frutos solo se separarían los estratos no esclerificados ubicados por debajo de la epidermis externa del pericarpio. Si bien se observaron algunas diferencias entre variedades o CC en los parámetros físicos del fruto (p<0,05), los mismos no se correlacionaron con la variabilidad observada en su AD. En todas las variedades, el contenido de materia grasa (MG) fue superior al 32% (p<0,01), excepto en L-8 (24,4%) y CW 99 OL (26,7%) en el primer CC, y al 41% en el segundo CC, excepto en L-8 (19,7%). La esclerificación total (ES) abarcó entre el 47 y 50% de los estratos del pericarpio en todas las variedades, excepto en Knowles 93079 (68,6%). El contenido FDN (85,7% vs 82,3%), de celulosa (C; 39,3% vs 37,3%) y LDA (22,5% vs 20,5%) del pericarpio fueron superiores en el segundo CC (p<0,01). La AD se correlacionó positivamente con el incremento en el contenido de PB (r = 0,75**) en la harina de frutos parcialmente descascarados con respecto a la harina de frutos enteros y negativamente con los porcentajes de ES (r= -0,48**), de FDN (r = -0,73**), C (r = -0,58**) y LDA (r = -0,82**) del pericarpio. La densidad de siembra fue la principal fuente de variación del rendimiento (A: 3841,1 kg ha-1; B: 3190,8 kg ha-1), del número de capítulos (A: 582,2; B: 386,9) y de frutos por unidad de superficie (A: 14832; B: 9777) en la variedad CW 99 OL (p<0,01). A su vez, el rendimiento fue menor (p<0,05) en el distanciamiento de 52 cm (3285,5 kg ha-1) que en el de 70 cm (3591,3 kg ha-1) y 35 cm (3671 kg ha-1). El peso de mil frutos (40,6 g en el primer CC y 41,7 g en el segundo CC) y su AD (70% en el primer CC y 60,3% en el segundo CC) no difirieron (p>0,05) entre ninguna de las distancias o densidades de siembra evaluadas. En esta tesis se analiza por primera vez la relación de la AD con parámetros estructurales del fruto y la calidad de la harina de frutos enteros y descascarados de distintas variedades de cártamo. Los resultados obtenidos indican que el descascarado mejoraría la calidad de la harina y que la reducción en el grosor y esclerificación del pericarpio observada en las variedades de cártamo de alto rendimiento de aceite incidiría positivamente en su AD, por lo que la selección a favor de ambos parámetros sería posible. / The Argentine oil seed industry has shown an increasing interest in hulling safflower fruit before the oil extraction process. Fruit dehulling would make it possible to decrease the volume of material circulating in the extraction system, the amount of waxes and pigments in the oil, and fiber content in the meal. The extent that hullability (H), the ease with which the pericarp separates from the seed relates to genetic variability and crop management has not been assessed. The objectives of this thesis were to assess during two consecutive growth cycles (GC): 1) the H of different safflower varieties grown in Argentina, 2) the quality of safflower meal obtained from dehulled and un-dehulled fruits, 3) the morphological characteristics of the fruits and the anatomic characteristics of the pericarp of different varieties and 4) how H is affected by different sowing rates and row spacing. The varietal trial was conducted at the Agricultural Experimental Station of INTA Hilario Ascasubi (Lat 39 ° 23'S, 62 ° 37 'W) and included seven safflower genotypes: Agrosearch L7-3, AG Phoenix, CW 99 OL, Arizona L7-5, 93079 Knowles, L-8, Seedtech S-345. The effect of two sowing rates (low (L): 7,5 kg ha-1 and high (H): 22,5 kg ha-1) and three row spacing (35 cm, 52 cm and 70 cm) on CW 99 OL fruit H was evaluated at the same experimental site. H of all varieties was between 15 and 46% higher in the first GC with respect to the second (p<0,01). H was similar among varieties (68 to 76% in the first GC and from 58 to 60% in the second one), except L-8 in the first GC and L-8 and Agrosearch L7-3 in the second GC (p<0,01). In all varieties, the percentage of mechanically extracted hull was 20-25% in the first GC and 16-21% in the second GC. Although fruit moisture at dehulling (5%) was similar between GC (p>0,05), fine production was reduced by half in the second GC (7% to 10,6%) with respect to the first GC (17% to 23%) in all varieties (p<0,01). Dehulling increased the content of proteins in the residual meal of all varieties, from 26% to 50% in the first GC and from 28% to 40% in the second (p<0,01). It also reduced the content of neutral detergent fiber (NDF) from 40,9% to 31,3% (p<0,01) and of two of its components (cellulose and hemicelluloses; p<0,05), as acid detergent lignin content (ADL) remained invariable (p>0,05). These results show that during fruit dehulling only the non-sclerified strata located below the pericarp outer epidermis would be separated. Although some differences were observed among varieties or GC in fruit physical parameters (p<0,05), they were not correlated with the variability observed in fruit H. Fruit fat content (FC) was higher than 32% (p<0,01), except in L-8 (24,4%) and CW 99 OL (26,7%) in the first GC, and 41% in the second GC, except in L-8 (19,7%). Total sclerification (S) comprised between 47 and 50% of the pericarp strata in all varieties, except in Knowles 93079 (68,6%). The content of NDF (85,7% vs 82,3%), cellulose (C; 39,3% vs 37,3%) and ADL (22,5% vs 20,5%) of the pericarp were higher in the second GC (p<0,01). H was correlated with the increase in protein content (r = 0,75 **) in the flour of partially dehulled fruit regarding whole fruit flour; and negatively with the percentage of S (r = -0,49 **), NDF (r = -0,73 **), C (r = -0,53 **) and ADL (r = -0,73 **) of the pericarp. Sowing rates was the main source of variation in crop yield (H: 3841,1 kg ha-1; L: 3190,8 kg ha-1) and capitulum (H: 582,2; L: 386,9) and fruit number per unit area (H: 14832; L: 9777; p<0,01). In turn, the yield was lower (p<0.05) in the 52 cm row spacing (3285,5 kg ha-1) than for the 70 cm (3591,3 kg ha-1) and 35 cm (3671 kg ha-1) row spacing. Thousand fruit weight (40,6 g in the first GC; 41,7 g in the second GC) and H (70% in the first GC; 60,3% in the second) did not differ (p>0,05) between any of the distances between rows or sowing rates assessed. This is the first analysis of the relationship of H with fruit structural parameters and meal quality of whole and dehulled fruits of different safflower varieties. The results obtained indicated that dehulling would improve residual meal quality and that pericarp thickness and sclerification reduction of the oil high yield safflower varieties would positively affect their H, so that selection in favor of both parameters would be beneficial.

Submitted by admin tede (tede@pucgoias.edu.br) on 2017-06-28T13:06:00Z No. of bitstreams: 1 LUCAS RODRIGUES SAMPAIO.pdf: 955041 bytes, checksum: 35594b1f525d8647d04413aaa06e3730 (MD5) / Made available in DSpace on 2017-06-28T13:06:00Z (GMT). No. of bitstreams: 1 LUCAS RODRIGUES SAMPAIO.pdf: 955041 bytes, checksum: 35594b1f525d8647d04413aaa06e3730 (MD5) Previous issue date: 2017-03-09 / The study of traditional uses of plants and their products has gradually increased during the last years, which has resulted in a significant number of publications in this area. Phytotherapics correspond to a significant principle of biologically ative natural products, many of which constituted models for the synthesis of a large number of drugs. Therefore, inserts it the Carthamus tinctorius, a plant that is part of the family Asteraceae, presenting themselves as a crop adapted to adverse conditions, being cultivated worldwide due to its medicinal properties. The Carthamus tinctorius contains a high quality oil rich in polyunsaturated fatty acids (PUFAs). The herb has potential for improvement through molecular breeding programs due to the availability of significant genetic and phenotypic diversity. In order to evaluate the angiogenic and genotoxic activities of safflower oil, angiogenesis tests were carried out on the chorioallantoid membrane (MCA) of embryonated chicken egg and micronucleus test on hematopoietic bone marrow of mice, where the results of the test in MCA Indicated that the oil caused a significant increase of the vascular network (p <0.05) in relation to the neutral and inhibitory controls. In the micronucleus test, the doses of 500, 1,000 and 1,500 mg.kg-1 were used. No genotoxic activity was observed at doses of 500 and 1,000, since the number of micronuclei in relation positive control doxorubicin (DXR) towas lower (p <0.05). However at the dose of 1,500, the genotoxic effect was observed, since the micronucleus values detected were similar to those of the positive control. In view of the techniques and conditions employed, the conclusions of this study reinforce that safflower oil presents therapeutic potential, with angiogenic activity and absence of genotoxicity in doses of 500 and 1,000 mg.kg-1. However, it should be consumed moderately because of the mutagenic activity found in high doses. / O estudo dos usos tradicionais de plantas e seus produtos têm aumentado gradualmente durante os últimos anos, o que resultou em um conjunto significativo de publicações nesta área. Os fitoterápicos correspondem a um princípio significativo de produtos naturais biologicamente ativos, muitos dos quais constituíram modelos para a síntese de um grande número de fármacos. Portanto, inclui-se o Carthamus tinctorius, planta que faz parte da família Asteraceae, apresentando-se como uma cultura adaptada a condições adversas, sendo cultivada em todo o mundo devido às suas propriedades medicinais. O Carthamus tinctorius, contém um óleo de alta qualidade rico em ácidos graxos poliinsaturados (PUFAs). A cultura da erva tem um potencial de melhoria através de programas de melhoramento molecular devido à disponibilidade da diversidade genética e fenotípica significativa. Com o intuito de avaliar as atividades angiogênica e genotóxica do óleo de cártamo, foram realizados os testes de angiogênese na membrana corioalantoide (MCA) do ovo embrionado de galinha e o teste de micronúcleo em medula óssea hematopoiética de camundongos, onde os resultados do ensaio na MCA indicaram que o óleo provocou aumento significativo da rede vascular (p<0,05) em relação aos controles neutro e inibidor. No teste do micronúcleo, foram utilizadas doses de, 500, 1.000 e 1.500 mg.kg-1. Não foi observada atividade genotóxica nas doses de 500 e 1.000, uma vez que o número de micronúcleos foi menor (p<0,05) em relação ao controle positivo doxorrubicina (DXR). Entretanto, na dose de 1.500 foi observado o efeito genotóxico, pois os valores de micronúcleos detectados foram semelhantes aos do controle positivo. Diante das técnicas e condições empregadas, as conclusões deste estudo reforçam que o óleo de cártamo apresenta potencial terapêutico, com atividade angiogênica e ausência de genotoxicidade nas doses de 500 e 1.000 mg.kg-1. No entanto, deve ser consumido moderadamente por conta da atividade genotóxica encontrada em doses elevadas.

Made available in DSpace on 2014-12-17T15:42:06Z (GMT). No. of bitstreams: 1 EllenKLM_DISSERT.pdf: 2963115 bytes, checksum: f31b2e203bf9ce3c07ef2636fa792275 (MD5) Previous issue date: 2012-09-20 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Most of the energy consumed worldwide comes from oil, coal and natural gas. These sources are limited and estimated to be exhausted in the future, therefore, the search for alternative sources of energy is paramount. Currently, there is considerable interest in making trade sustainable biodiesel, a fuel alternative to fossil fuels, due to its renewable nature and environmental benefits of its use in large scale. This trend has led the Brazilian government to establish a program (Probiodiesel) with the aim of introducing biodiesel into the national energy matrix, by addition of 5% biodiesel to conventional diesel in 2010 to foster not only the increase of renewable energy, but reduce imports of crude oil. This work evaluates different methods of extraction of oil Carthamus tinctorius L., their characterization by IR, 1H and 13C NMR, HPLC and TG and their use in the production of methyl ester (molar ratio of oil / alcohol 1:6, and NaOH catalyst). The physico-chemical parameters (acid value, density, viscosity, saponification index and surface tension) of oil and biodiesel were also described. The produced biodiesel had a yield of 93.65%, was characterized in relation to their physicochemical properties showing satisfactory results (density=875 kg/m3, viscosity = 6.22 mm2/s, AI = 0.01 mg (NaOH) /g) compared with the values established by the the National Agency Oil, Natural Gas and Biofuels / A maior parte da energia consumida no mundo prov?m do petr?leo, do carv?o e do g?s natural. Essas fontes s?o limitadas e com previs?o de esgotamento no futuro, portanto, a busca por fontes alternativas de energia ? de suma import?ncia. Atualmente, h? um consider?vel interesse em tornar sustent?vel o com?rcio do biodiesel, um combust?vel alternativo ao de origem f?ssil, em fun??o de sua natureza renov?vel e dos benef?cios ambientais de seu emprego em larga escala. Essa tend?ncia levou o governo brasileiro a estabelecer um programa (Probiodiesel) com o objetivo de introduzir o biodiesel na matriz energ?tica nacional, atrav?s da adi??o de 5% de biodiesel ao diesel convencional em 2010, de forma a incentivar n?o somente o aumento de fontes renov?veis de energia, mas reduzir as importa??es do ?leo bruto. O presente trabalho avalia diferentes m?todos de extra??es (Soxhlet, Ultrassom e Exaustiva) do ?leo de Carthamus tinctorius L., sua caracteriza??o por IV, RMN 1H e 13C, CLAE, TG e Estabilidade Oxidativa que na presen?a de antioxidante demonstrou ser bastante satisfat?rio e seu emprego na produ??o de ?ster met?lico (raz?o molar ?leo/?lcool; 1:6; NaOH como catalisador). Os par?metros fisico-qu?micos (?ndice de acidez, densidade, viscosidade, ?ndice de saponifica??o e tens?o superficial) do ?leo e biodiesel tamb?m est?o descritos. O biodiesel produzido que apresentou rendimento de 93,65%, foi caracterizado em rela??o as suas propriedades fisico-qu?micas apresentando resultados satisfat?rios (Densidade = 875 kg/m3; Viscosidade = 6,22 mm2/s; IA = 0,01 mg (NaOH)/g) quando comparados com os valores estabelecidos pela Ag?ncia Nacional do Petr?leo, G?s Natural e Biocombust?veis

Submitted by FERNANDO VIEIRA COSTA GUIDORIZZI null (fernando_guidorizzi@hotmail.com) on 2016-05-02T19:07:11Z No. of bitstreams: 1 Fernando Guidorizzi - Dissertação Definitiva.pdf: 2781433 bytes, checksum: f56e6ea73314244bb7d600cdfc7a26d0 (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-05-04T18:43:58Z (GMT) No. of bitstreams: 1 guidorizzi_fvc_me_bot.pdf: 2781433 bytes, checksum: f56e6ea73314244bb7d600cdfc7a26d0 (MD5) / Made available in DSpace on 2016-05-04T18:43:58Z (GMT). No. of bitstreams: 1 guidorizzi_fvc_me_bot.pdf: 2781433 bytes, checksum: f56e6ea73314244bb7d600cdfc7a26d0 (MD5) Previous issue date: 2016-02-25 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / O cártamo é uma oleaginosa que vem despertando interesse na agricultura nacional, pois se apresenta como uma opção para o cultivo de segunda safra (outono-inverno). Entretanto, para expansão do cultivo de cártamo no Brasil faz-se necessário à realização de estudos abordando o seu crescimento e absorção de nutrientes nas suas fases de desenvolvimento, em diferentes condições de cultivo, pois o conhecimento sobre essas variáveis permitirá precisão na recomendação de adubação para a cultura. Assim, objetivou-se com este trabalho avaliar o acúmulo de matéria seca (MS) e de macronutrientes durante o ciclo, produtividade de grãos e exportação de macronutrientes por genótipos de cártamo em função da aplicação de nitrogênio (N) em cobertura, em sistemas plantio direto (SPD) com diferentes tempos de adoção. O experimento foi conduzido no município de Botucatu-SP, em um Nitossolo Vermelho distrófico. Foi adotado o delineamento em blocos casualizados no esquema de parcelas subdivididas, com quatro repetições. As parcelas foram compostas por oito tratamentos obtidos a partir da combinação de dois tempos adoção do SPD (SPDR - sistema plantio direto recém-implantado e SPDC - sistema plantio direto consolidado, com 28 anos desde a implantação), dois genótipos de cártamo (IMA 2234 e IMA 2237) e dois níveis de N em cobertura (0 e 100 kg ha-1). As subparcelas foram compostas por sete épocas de amostragens (de 22 dias após a emergência (DAE) até o final do ciclo da cultura). Ambos os genótipos de cártamo tiveram lento acúmulo de MS e macronutrientes durante o estádio de roseta (0 a 22 DAE). A partir desse estádio até o início do enchimento de grãos (entre 84 e 111 DAE) ocorreu o período de maior crescimento e acúmulo de macronutrientes pela cultura. O genótipo IMA 2234 apresentou maiores quantidades de MS, N, P, K e S acumuladas na parte aérea. A adubação nitrogenada de cobertura influenciou, principalmente, a absorção de N. Os componentes da produção, a produtividade de grãos e a exportação de macronutrientes não foram influenciados pelos genótipos, tempos de adoção do SPD ou adubação nitrogenada. A extração de macronutrientes, na média dos tratamentos, foi: K (168 kg ha-1) > N (149 kg ha-1) > Ca (125 kg ha-1) > S (15 kg ha-1) > Mg (14 kg ha-1) > P (13 kg ha-1). A exportação de macronutrientes pelos grãos seguiu a sequência, na média dos tratamentos: N (44 kg ha-1) > K (12 kg ha-1) > Ca (6 kg ha-1) > P (4 kg ha-1) > Mg (3 kg ha-1) > S (2 kg ha-1). / Safflower is an oilseed that has attracted national interest in agriculture, as is presented as an option for the second-crop cultivation (fall-winter). However, for expansion of safflower cultivation in Brazil is a need for studies addressing their growth and nutrient uptake in their stages of development in different growth conditions, as the knowledge of these variables will enable precision in fertilizer recommendation for the crop. Therefore, the aim was to evaluate the accumulation of dry matter (DM) and macronutrients over the cycle, grain yield, and macronutrient removal by safflower genotypes as affected by sidedressed nitrogen (N) fertilization in no-till system (NTS) with different adoption times. The experiment was conducted in Botucatu-SP, in a Typic Rhodudalf soil. It was adopted a randomized block design in a split-plot scheme with four replications. The plots were composed by eight treatments obtained from the combination of two adoption times of the NTS (NNTS - a newly implemented no-tillage system and ENTS - an established no-till system, with 28 years since the establishment), two safflower genotypes (IMA 2234 and IMA 2237) and two levels of sidedressed N fertilization in topdressing (0 and 100 kg ha-1). The subplots were composed of seven sampling dates (from 22 days after emergence (DAE) by the end of the cycle). Both safflower genotypes had slow accumulation of DM and macronutrients during the rosette stage (0-22 DAE). From this stage until the early grain filling (between 84 and 111 DAE) was the period of greatest growth and accumulation of macronutrients by crop. The genotype IMA 2234 have higher amounts of DM, N, P, K and S accumulated in the shoot. Sidedressed N fertilization influenced mainly N uptake. The yield components, grain yield, and nutrient removal were not influenced by genotype, adoption times of the NTS or sidedressed N fertilization. The uptake of macronutrient was, on average of treatments: K (168 kg ha-1) > N (149 kg ha-1) > Ca (125 kg ha-1) > S (15 kg ha-1) > Mg (14 kg ha-1) > P (13 kg ha-1). The removal of macronutrients by grains followed the sequence, on average of treatments: N (44 kg ha-1) > K (12 kg ha-1) > Ca (6 kg ha-1) > P (4 kg ha -1) > Mg (3 kg ha-1) > S (2 kg ha-1). / FAPESP: 2014/20301-8