No description available.

Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 154-160). Also available via the Internet.

Células neuronais cocultivadas com células-tronco derivadas do tecido adiposo (ADSC) podem induzir estas últimas à transdiferenciação neuronal. No entanto, os processos de comunicação celular envolvidos nessa indução, e a funcionalidade da ADSC transdiferenciada in vivo, são desconhecidos. Recentemente, um novo tipo de comunicação celular mediada por vesículas extracelulares são indicados na modulação de diferentes eventos celulares como a diferenciação. Portanto, a hipótese, nesta proposta, foi identificar se o processo de diferenciação celular é mediado por vesículas extracelulares e se as células diferenciadas são capazes de atuar na regeneração de tecidos nas lesões do sistema nervoso periférico. Para tal, essa pesquisa foi dividida em duas fases: a primeira consiste no processo in vitro, com o objetivo de observar o processo de transição da ADSC para a linhagem neuronal e analisar a função da comunicação celular no processo de diferenciação; a segunda consistiu na avaliação in vivo da possível funcionalidade das ADSC diferenciadas. O camundongo foi o modelo animal utilizado (C57BL/6 e FVB). As ADSC e as células neuronais foram isoladas, cultivadas em cultivo primário e cocultivadas durante três, sete e 14 dias. Para a comprovação das mudanças fenotípicas das ADSC, realizou-se a imunolocalização com beta tubulina III e SNAP25 e PCR em tempo real (RT-qPCR) dos genes Map2 e Snap25. Seguido de analises de genes relacionados com a neurogênese. Adicionalmente, as vesículas extracelulares foram isoladas e utilizados para análises in vitro da diferenciação e análises gênicas e funcionais. Como resultado, verificou-se que as ADSC em cocultivo com neurônios podem se diferenciar em neuronais-like. Além disso, comprovou-se a comunicação por vesículas extracelulares entre neurônios e ADSC, e as vesículas extracelulares foram correlacionadas neste processo, pelo transporte da proteína SNAP25. Após estes resultados prosseguiu-se para a segunda fase deste trabalho, a etapa in vivo, que incidiu na utilização das ADSC cocultivadas por sete dias e avaliação funcional local e sistêmica no processo de regeneração do nervo ciático após neurotmese. Como resultado desta etapa as células-tronco cocultivadas modularam a lesão, e proporcionaram uma melhoria na funcionalidade após lesão. Conclui-se, através desta pesquisa, que as ADSC diferenciadas em neuronais-like, sob indução dos neurônios e suas vesículas extracelulares podem ser uma fonte celular alternativa no auxílio na regeneração de nervos periféricos. / Neuronal cells co-cultured with stem cells derived from adipose tissue (ADSC) can induce the latter to neuronal transdifferentiation. However, the cellular communication processes involved in this induction, and the functionality of the transdifferentiated ADSC in vivo, are unknown. Recently, a new type of cellular communication measured by extracellular vesicles was indicated in the modulation of different cellular events like differentiation. Therefore, the hypothesis in this proposal was to identify if the process of cellular differentiation is mediated by extracellular vesicles and if the differentiated cells are able to act in the regeneration of tissues in the lesions of the peripheral nervous system. For this, the research was divided in two phases: the first one consisted of the in vitro process, with the objective of observing the transition process of the ADSC to the neuronal lineage and analyzing the cellular communication function in the differentiation process; the second consisted in the in vivo evaluation of the possible functionality of the differentiated ADSCs. Murine was the animal model used (C57BL/6 and FVB). ADSCs and neuronal cells were isolated, cultured in primary culture and co-cultured for three, seven and 14 days. To confirm the phenotypic changes of ADSC, immunolocalization with beta tubulin III and SNAP25 and real-time PCR (RT-qPCR) of the Map2 and Snap25 genes was performed, followed by analysis of genes related to neurogenesis. In addition, extracellular vesicles were isolated and used for in vitro differentiation and gene and functional analysis. As a result, it has been found that ADSCs in co-culture with neurons can differentiate into neuronal-like. The communication by extracellular vesicles between neurons and ADSCs was verified, and the extracellular vesicles were correlated in the differentiation process by the transport of the protein SNAP25. After these results, the second phase of this work was continued, the in vivo step, which focused on the use of the co-cultivated ADSCs for seven days and functional local and systemic evaluation in the process of sciatic nerve regeneration after neurotmese. As a result of this step, the co-cultured stem cells modulated the lesion and provided an improvement in functionality after injury. It is concluded that ADSCs can transdifferentiate neuronal lines in co-culture with neurons, the extracellular vesicles play a certain role in this process and the transdifferentiated ADSC may be an alternative to aid in the regeneration of peripheral nerves.

Thesis (M.App.Sc.) - Carleton University, 2007. / Includes bibliographical references (p. 117-125). Also available in electronic format on the Internet.

Células neuronais cocultivadas com células-tronco derivadas do tecido adiposo (ADSC) podem induzir estas últimas à transdiferenciação neuronal. No entanto, os processos de comunicação celular envolvidos nessa indução, e a funcionalidade da ADSC transdiferenciada in vivo, são desconhecidos. Recentemente, um novo tipo de comunicação celular mediada por vesículas extracelulares são indicados na modulação de diferentes eventos celulares como a diferenciação. Portanto, a hipótese, nesta proposta, foi identificar se o processo de diferenciação celular é mediado por vesículas extracelulares e se as células diferenciadas são capazes de atuar na regeneração de tecidos nas lesões do sistema nervoso periférico. Para tal, essa pesquisa foi dividida em duas fases: a primeira consiste no processo in vitro, com o objetivo de observar o processo de transição da ADSC para a linhagem neuronal e analisar a função da comunicação celular no processo de diferenciação; a segunda consistiu na avaliação in vivo da possível funcionalidade das ADSC diferenciadas. O camundongo foi o modelo animal utilizado (C57BL/6 e FVB). As ADSC e as células neuronais foram isoladas, cultivadas em cultivo primário e cocultivadas durante três, sete e 14 dias. Para a comprovação das mudanças fenotípicas das ADSC, realizou-se a imunolocalização com beta tubulina III e SNAP25 e PCR em tempo real (RT-qPCR) dos genes Map2 e Snap25. Seguido de analises de genes relacionados com a neurogênese. Adicionalmente, as vesículas extracelulares foram isoladas e utilizados para análises in vitro da diferenciação e análises gênicas e funcionais. Como resultado, verificou-se que as ADSC em cocultivo com neurônios podem se diferenciar em neuronais-like. Além disso, comprovou-se a comunicação por vesículas extracelulares entre neurônios e ADSC, e as vesículas extracelulares foram correlacionadas neste processo, pelo transporte da proteína SNAP25. Após estes resultados prosseguiu-se para a segunda fase deste trabalho, a etapa in vivo, que incidiu na utilização das ADSC cocultivadas por sete dias e avaliação funcional local e sistêmica no processo de regeneração do nervo ciático após neurotmese. Como resultado desta etapa as células-tronco cocultivadas modularam a lesão, e proporcionaram uma melhoria na funcionalidade após lesão. Conclui-se, através desta pesquisa, que as ADSC diferenciadas em neuronais-like, sob indução dos neurônios e suas vesículas extracelulares podem ser uma fonte celular alternativa no auxílio na regeneração de nervos periféricos. / Neuronal cells co-cultured with stem cells derived from adipose tissue (ADSC) can induce the latter to neuronal transdifferentiation. However, the cellular communication processes involved in this induction, and the functionality of the transdifferentiated ADSC in vivo, are unknown. Recently, a new type of cellular communication measured by extracellular vesicles was indicated in the modulation of different cellular events like differentiation. Therefore, the hypothesis in this proposal was to identify if the process of cellular differentiation is mediated by extracellular vesicles and if the differentiated cells are able to act in the regeneration of tissues in the lesions of the peripheral nervous system. For this, the research was divided in two phases: the first one consisted of the in vitro process, with the objective of observing the transition process of the ADSC to the neuronal lineage and analyzing the cellular communication function in the differentiation process; the second consisted in the in vivo evaluation of the possible functionality of the differentiated ADSCs. Murine was the animal model used (C57BL/6 and FVB). ADSCs and neuronal cells were isolated, cultured in primary culture and co-cultured for three, seven and 14 days. To confirm the phenotypic changes of ADSC, immunolocalization with beta tubulin III and SNAP25 and real-time PCR (RT-qPCR) of the Map2 and Snap25 genes was performed, followed by analysis of genes related to neurogenesis. In addition, extracellular vesicles were isolated and used for in vitro differentiation and gene and functional analysis. As a result, it has been found that ADSCs in co-culture with neurons can differentiate into neuronal-like. The communication by extracellular vesicles between neurons and ADSCs was verified, and the extracellular vesicles were correlated in the differentiation process by the transport of the protein SNAP25. After these results, the second phase of this work was continued, the in vivo step, which focused on the use of the co-cultivated ADSCs for seven days and functional local and systemic evaluation in the process of sciatic nerve regeneration after neurotmese. As a result of this step, the co-cultured stem cells modulated the lesion and provided an improvement in functionality after injury. It is concluded that ADSCs can transdifferentiate neuronal lines in co-culture with neurons, the extracellular vesicles play a certain role in this process and the transdifferentiated ADSC may be an alternative to aid in the regeneration of peripheral nerves.

Mobile handset manufacturers have begun to include wireless LAN (WLAN) interfaces in their cellular handsets. This allows users to access WLAN networks when they are available and to revert to conventional cellular communications otherwise. In this way the handset can dynamically use the "best" available network, by switching connections between the two network interfaces. This switching is referred to as a vertical handoff (VHO). When handling real-time connections, handsets must be capable of performing a seamless vertical handoff. This occurs when the interface switching does not disrupt the quality of service requirements of the active connections. Vertical handoffs are generally time consuming, and this delay creates a difficult problem since WLAN coverage can be lost very abruptly. In this thesis, we propose and investigate several methods of mitigating this problem. A solution based on using a Vertical Handoff Support Node (VHSN) is proposed. When the WLAN link is lost, the VHSN is able to quickly redirect packets through the local cellular base-station during the time that handoff is taking place. This approach can eliminate VHO link loss. It is shown that the act of WLAN-to-cellular handoff can result in a severe bandwidth deficit problem on the WLAN. A novel bandwidth reservation and securing mechanism is proposed which overcomes this problem and performs significantly better than schemes based on modified versions of the static guard channel scheme used in cellular networks. The work is characterized by simulation and analytic models which investigate the key performance aspects of this type of system. / Thesis / Doctor of Philosophy (PhD)

Thesis (Ph.D.)--University of Melbourne, Dept. of Electrical and Electronic Engineering, 2007. / Typescript. Includes bibliographical references (leaves 143-154).

With the rapid advances of technology, digitisation of many facets of our existence is taking place in an attempt to improve everyday life. The automotive industry is following suit, attempting to introduce connected traffic technology that is meant to improve traffic fluidity and safety. To facilitate this, connected vehicles aim to create solutions for the sharing of information between other vehicles, infrastructure - such as traffic light controllers, and pedestrians. In an attempt to further investigate the connected vehicle landscape of today, the thesis compared the two most prominent technologies, DSRC and cellular communication. An essential part of this comparison was highlighting the potential attacks that the two technologies could be exposed to. This was done in order to open up a discussion on what technology is the most suitable to focus on for the future both in terms of viability and security. DSRC has been considered the prominent communication technology for connected vehicles, but the development has stagnated. As such, the ever-evolving cellular technology is looking like the superior technology. This, however, is reliant on 5G delivering the speeds, stability and security promised. The state of constant vehicular connection is going to lead to many issues and concerns, both for the privacy of the individual but also the safety of the public. While connected traffic aims to solve a number of issues from traffic accidents to emissions - if the security of the communication is not constantly evolving to meet the rapid development of new technology, the consequences of connecting such a delicate system might nullify the potential benefits.

Les amibes libres sont des protozoaires retrouvés dans de nombreux environnements où ils ingèrent par phagocytose des bactéries, des champignons, des virus ou d'autres protozoaires. Le modèle d'étude principal de cette thèse, divisée en deux grandes parties, a été Acanthamoeba castellanii. La première partie de la thèse a concerné l'étude de la préférence trophique des amibes, en présence de différents micro-organismes. Parmi ceux-ci, deux bactéries, Klebsiella pneumoniae et Staphylococcus aureus, se sont montrées les plus attractives pour les protozoaires étudiés. Des extraits bactériens ont été fractionnés et leur étude a permis de mettre en évidence la nature protéique des composés chimioattractifs impliqués dans ce dialogue intergenre. Certaines données de la littérature ont rapporté les similitudes entre A. castellanii et d'autres cellules phagocytaires que sont les macrophages. La seconde partie de la thèse a permis de comparer les activités de phagocytose d'A. castellanii et de la lignée macrophagique Thp-1 vis-à-vis de quatre micro-organismes : le champignon filamenteux Aspergillus fumigatus, les bactéries Klebsiella pneumoniae et Staphylococcus aureus, et un Adénovirus sérotype B3. L'influence des deux types de cellules phagocytaires sur la croissance des micro-organismes a également été étudiée. Ces travaux ont permis de mettre en évidence des différences de comportements des amibes libres par rapport aux macrophages vis-à-vis de micro-organismes pathogènes, montrant qu'il n'est pas toujours possible d'extrapoler les résultats d'études amibes libres/micro-organismes aux relations macrophages/micro-organismes. / Free living amoebae (FLA) are protoza found in various environments where they can feed by phagocytosis on bacteria, fungi, viruses or other protozoa. Acanthamoebae castellanii was used as the main model in this thesis, divided in two parts. The first part of the thesis relied on the trophic preference of amoebae, in presence of different microorganisms. Amongst them, two bacteria, Klebsiella pneumoniae and Staphylococcus aureus, appeared as the most attractive for the studied protozoa. Bacterial extracts have been fractionned and their study has shown the protein nature of the chemoattractants involved in this interspecies crosstalk.Literature data have reported similarities between A. castellanii and other phagocytic cells such as macrophages. The second part of the thesis allowed us to compare phagocytic activities of A. castellanii and Thp-1 macrophagic cells towards four microorganisms: the filamentous fungus Aspergillus fumigatus, the bacteria Klebsiella pneumoniae and Staphylococcus aureus and an Adenovirus B3 serotype. The influence of the two phagocytic cells on the microorganisms' growth has also been investigated. This has evidenced the behavior differences between FLA and macrophages towards pathogenic microorganisms, showing that results obtained by studying amoebae and microorganisms relationships could not be extrapolated in all cases to the relationships between macrophages and microorganisms.

O crescente aumento da demanda de tráfego nas redes celulares vem aumentando a necessidade de uma melhor utilização dos recursos do sistema, já que sua expansão é custosa. Nas estações rádio base (ERB), a disponibilidade de largura de faixa de freqüências é limitada e desta maneira, em uma rede de comunicação móvel celular, o controle de admissão de chamadas exerce grande influência no desempenho do sistema, pois determina a utilização de banda das ERBs e se uma determinada quantidade de recursos (banda) será alocado ou não para uma determinada chamada. O desempenho da rede pode ser atrelado a determinados parâmetros, como a probabilidade de bloqueio de novas chamadas, probabilidade de bloqueio de chamadas handoff e a utilização de banda da rede. Este trabalho propõe um controle de admissão de chamadas que, no atendimento de uma chamada, faz o empréstimo de banda de chamadas em andamento na célula no caso de banda insuficiente. O sistema adota um mecanismo heurístico que determina a banda disponível para novas chamadas conforme os valores de certos parâmetros do sistema. O empréstimo de banda é realizado em chamadas em andamento nas células até níveis mínimos estabelecidos para cada tipo de chamada, que se diferenciam pelas necessidades de banda de cada uma. O algoritmo foi aplicado às bandas e características de uma rede de terceira geração (3G), que possui chamadas de voz, videoconferência, interação multimídia, e-mail, downloads e transferência de arquivos e a uma rede GSM/GPRS (global system for mobile communications/ general packet radio service), que possui chamadas de voz e de dados. Os resultados mostram melhorias na probabilidade de bloqueio de novas chamadas, probabilidade de bloqueio de handoff e na utilização de banda do sistema. / The recent growth in traffic loads in cellular networks has seen the need for a better use of system resources as its expansion is expensive. In the base transceiver station (BTS), the bandwidth availability is limited. Thus, in cellular networks the call admission control greatly influences the system performance because it determines the bandwidth use of the BTSs and if an amount of resources will or will not be allocated to a call. The network performance can be evaluated by parameters such as blocking probability of new calls, dropping probability of handoff calls and bandwidth use. This work proposes a call admission control that carries out the bandwidth borrowing when a call arrives and there is not enough bandwidth. The system makes use of a heuristic mechanism that determines the available bandwidth for the new calls according to some parameter values of the system. The bandwidth borrowing is applied to the cell ongoing calls until the minimum levels for each type are met. The algorithm was applied to the bandwidths and characteristics of a third generation cellular network, which supports voice calls, videoconference, multimedia interaction, e-mails, downloads and file transfers. It was also applied to a GSM/GPRS (global system for mobile communications/ general packet radio service), which supports voice and data calls. The results show improvements in the blocking probability of new calls, dropping probability of handoff calls and in the bandwidth use of the system.