Diss. (sammanfattning) Uppsala : Uppsala universitet, 2006. / Härtill 5 uppsatser.

Law, Pak Tsun = 研究氧化鋅納米粒子應用在生物醫學的可能性 / 羅百浚. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (p. 62-63). / Abstracts in English and Chinese. / Law, Pak Tsun = Yan jiu yang hua xin na mi li zi ying yong zai sheng wu yi xue de ke neng xing / Luo Baijun. / Chapter 1 --- Introduction --- p.1 / Chapter 2 --- Background --- p.2 / Chapter 2.1 --- Introduction of Zinc Oxide Nanostructures --- p.2 / Chapter 2.1.1 --- Biomedical Application of ZnO nanostructure --- p.3 / Chapter 2.2 --- Current problems of Zinc Oxide Nanostructures on Biological Application --- p.5 / Chapter 2.2.1 --- Behaviours of ZnO Nanostructure in Cell Culture --- p.6 / Chapter 2.2.2 --- Cytotoxicity of ZnO Nanostructures --- p.8 / Chapter 2.2.3 --- Other Synthetic Problems --- p.9 / Chapter 2.3 --- Summary --- p.9 / Chapter 3 --- Instrumentation and Experimental Methodology --- p.11 / Chapter 3.1 --- Electron Microscopy --- p.11 / Chapter 3.1.1 --- SEM --- p.12 / Chapter 3.1.2 --- TEM --- p.13 / Chapter 3.1.3 --- Elemental Analysis --- p.14 / Chapter 3.2 --- X-ray Photoelectron Spectroscopy --- p.16 / Chapter 3.3 --- X-ray Diffraction --- p.17 / Chapter 3.4 --- Dynamic Light Scattering --- p.18 / Chapter 3.5 --- Photoluminescence Spectroscopy --- p.19 / Chapter 3.6 --- MTT Assay --- p.20 / Chapter 3.7 --- Cells Incubation --- p.22 / Chapter 4 --- Synthesis and Characterization of Zinc Oxide Nanostructure --- p.23 / Chapter 4.1 --- Hydrophobic Zinc Oxide Nanoparticle --- p.23 / Chapter 4.1.1 --- Synthetic Method --- p.23 / Chapter 4.1.2 --- Characterizations and Property measurements of the ZnO nanoparticles --- p.24 / Chapter 4.2 --- Hydrophilic Zinc Oxide Nanoparticle --- p.29 / Chapter 4.2.1 --- Synthetic Method --- p.29 / Chapter 4.2.2 --- Characterizations and property measurements of the ZnO nanoparticles --- p.30 / Chapter 5 --- Interaction of Zinc Oxide with Human Cells --- p.35 / Chapter 5.1 --- Can Zinc Oxide Nanoparticles Enter the cells? --- p.35 / Chapter 5.1.1 --- Exp erimental --- p.36 / Chapter 5.1.2 --- General Description --- p.36 / Chapter 5.2 --- What Affect the Cellular Uptake of ZnO Nanoparticles? --- p.37 / Chapter 5.2.1 --- Concentration of ZnO Nanoparticles --- p.37 / Chapter 5.2.2 --- Agglomeration of ZnO nanoparticles --- p.40 / Chapter 5.2.3 --- Reaction with Buffer Solution and Culture Medium --- p.41 / Chapter 5.2.4 --- Summary --- p.45 / Chapter 5.3 --- Cell Viability after Introducing ZnO Nanoparticle to the Cells ´Ø --- p.56 / Chapter 5.3.1 --- Experimental --- p.56 / Chapter 5.3.2 --- Results and Discussion --- p.57 / Chapter 6 --- Conclusion --- p.59 / Bibliography --- p.62 / Chapter A --- TEM sample preparation procedure for animal cell --- p.64

Made available in DSpace on 2014-06-11T19:31:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2005Bitstream added on 2014-06-13T19:01:30Z : No. of bitstreams: 1 scudeller_pso_dr_botfmvz.pdf: 2044336 bytes, checksum: 533c13f118e2a550bd3a71ed20c31cc2 (MD5) / A doença podal é considerada como a terceira mais importante enfermidade nos bovinos leiteiros, após mastite e problemas reprodutivos, levando a uma perda econômica e financeira significativa. Pesquisadores observaram uma correlação negativa de enfermidades podais com produção leiteira e com a eficiência reprodutiva. O propósito deste estudo foi conhecer a ocorrência, tipo e grau de enfermidades falangeanas assintomáticas diagnosticadas pela radiografia, de fêmeas da espécie bovina Bos indicus, da raça Nelore, provenientes do Centro Nacional de Pesquisa do Gado de Corte da EMBRAPA do município de Campo Grande - MS, criadas em sistema extensivo, pastagem de Brachiaria decumbens, com suplementação mineral e controle sanitário e parasitário rigorosos, e correlacionar o grau de alterações ósseas com o peso vivo, circunferência torácica, DMO, proteínas totais, CK, GGT, albumina, globulina, AST, Ca, P, Mg, Mn, Zn, fosfatase alcalina, osteocalcina, leucograma e o pH rumenal. Foram radiografados os cascos dos quatro membros nas posições dorso-palmar, dorso-plantar e látero-medial e o terceiro osso metacarpo na posição dorso-palmar para avaliação densitométrica. Pela avaliação estatística, não ocorreu correlação entre as variáveis obtidas e lesões ósseas podais. Apenas ocorreu correlação entre idade e presença de exostose podal. Ficou comprovada a importância do exame radiográfico como imprescindível meio de diagnostico para a laminite assintomática. / The pedal disease has been as the third most important disease in dairy cattle, after mastitis and reproduction problems, with a significant economical and financial loss. Workers observed a negative correlation of feet diseases with dairy yield and reproductive performance. The purpose of this study was know the occurrence and type and degree of pathological bone chances in phalangies without clinical signs diagnosed through radiographic examine of bovine females species Bos indicus, from Center National of Research of Beef Bovine, EMBRAPA, city Campo Grande - MS, husbandry in extensive system, pasture of Brachiaria decumbens, with mineral supplement and rigorous parasitic and sanitary control; and to correlate the degree osseus changes with weight, toracic circumference, BMD, Protein total serum, CK, GGT, Albumin, Globulin, AST, Ca, P, Mg, Mn, Zn, Alkaline Phosphatase, Osteocalcin, White blood cells and ruminal pH. The claws of fore and hind of cows were radiographed in dorso-palmar, dorso-plantar and latero-medial views, and the bone metacarpus was radiographed in dorso-palmar view to evaluation densitometric. With statistical analysis did not occur correlation between samples obtained and pedal bone lesions. Occur correlation between age and pedal exostosis. It was confirmed the importance of radiographic examine as recourse diagnosis indispensable to subclinic laminitis.

Resumo: A doença podal é considerada como a terceira mais importante enfermidade nos bovinos leiteiros, após mastite e problemas reprodutivos, levando a uma perda econômica e financeira significativa. Pesquisadores observaram uma correlação negativa de enfermidades podais com produção leiteira e com a eficiência reprodutiva. O propósito deste estudo foi conhecer a ocorrência, tipo e grau de enfermidades falangeanas assintomáticas diagnosticadas pela radiografia, de fêmeas da espécie bovina Bos indicus, da raça Nelore, provenientes do Centro Nacional de Pesquisa do Gado de Corte da EMBRAPA do município de Campo Grande - MS, criadas em sistema extensivo, pastagem de Brachiaria decumbens, com suplementação mineral e controle sanitário e parasitário rigorosos, e correlacionar o grau de alterações ósseas com o peso vivo, circunferência torácica, DMO, proteínas totais, CK, GGT, albumina, globulina, AST, Ca, P, Mg, Mn, Zn, fosfatase alcalina, osteocalcina, leucograma e o pH rumenal. Foram radiografados os cascos dos quatro membros nas posições dorso-palmar, dorso-plantar e látero-medial e o terceiro osso metacarpo na posição dorso-palmar para avaliação densitométrica. Pela avaliação estatística, não ocorreu correlação entre as variáveis obtidas e lesões ósseas podais. Apenas ocorreu correlação entre idade e presença de exostose podal. Ficou comprovada a importância do exame radiográfico como imprescindível meio de diagnostico para a laminite assintomática. / Abstract: The pedal disease has been as the third most important disease in dairy cattle, after mastitis and reproduction problems, with a significant economical and financial loss. Workers observed a negative correlation of feet diseases with dairy yield and reproductive performance. The purpose of this study was know the occurrence and type and degree of pathological bone chances in phalangies without clinical signs diagnosed through radiographic examine of bovine females species Bos indicus, from Center National of Research of Beef Bovine, EMBRAPA, city Campo Grande - MS, husbandry in extensive system, pasture of Brachiaria decumbens, with mineral supplement and rigorous parasitic and sanitary control; and to correlate the degree osseus changes with weight, toracic circumference, BMD, Protein total serum, CK, GGT, Albumin, Globulin, AST, Ca, P, Mg, Mn, Zn, Alkaline Phosphatase, Osteocalcin, White blood cells and ruminal pH. The claws of fore and hind of cows were radiographed in dorso-palmar, dorso-plantar and latero-medial views, and the bone metacarpus was radiographed in dorso-palmar view to evaluation densitometric. With statistical analysis did not occur correlation between samples obtained and pedal bone lesions. Occur correlation between age and pedal exostosis. It was confirmed the importance of radiographic examine as recourse diagnosis indispensable to subclinic laminitis. / Orientador: Lucy Marie Ribeiro Muniz / Coorientador: Pedro Paulo Pires / Doutor

Note: / Clinical chemistry is a medical discipline whose aim is to diagnose and assess disease by analysis of biological specimens. Modem laboratories can perform several hundred different tests using many different methods developed over the last century. The classical, more traditional assays are typically labour-intensive, not multiplexed (only measure one analyte or disorder per assay), expensive, require a long turnaround time, and may not provide adequate sensitivity and specificity. Developments in mass spectrometry (MS) and related technologies over the last two decades have provided solutions for many if not all of these shortcomings. While MS based applications have not yet been widely implemented in clinical chemistry laboratories, current developments will encourage the replacement of traditional methods as well as the expansion of clinically diagnostic endpoints. Indeed, modem MS can be used to simultaneously analyze and quantitate multiple biomarkers in a single analysis. Currently, no other technique exists that can provide a comparable multiplexed analysis. In this thesis, current MS and related technologies were developed and applied to several important but distinct clinical chemistry applications. [...] / La chimie clinique est une discipline medicale qui a pour but de diagnostiquer la presence et la progression d'une maladie par l'analyse d'echantillons biologiques. Les laboratoires modemes peuvent executer des centaines d'analyses en utilisant plusieurs methodes developpees au courrant des cent demieres annees. Les essaisc1assiques, et plus traditionnels, sont souvent laborieux, non multiplexe (mesurent seulement un analyte par essai), cher, exige un long temps de rotation et risque de ne pas fournir une specificite adequate. Pendant les deux dernieres decennies, les developpements dans Ie domaine de la spectrometrie de masse (MS) et les technologies rattachees ont foumi des solutions a plusieurs, pour ne pas dire tous, manques retrouves dans les methodes d'analyse traditionnelles.

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