Dois experimentos foram conduzidos de forma semelhante, variando apenas a idade do lote de matrizes Ross que originou os ovos (42 e 60 semanas de idade, respectivamente, para os experimentos 1 e 2), com o objetivo de avaliar o desempenho de pintos oriundos de ovos leves e pesados, alimentados com dietas pré-iniciais de diferentes formas físicas e níveis nutricionais.. Foram incubados ovos apresentando peso médio de 59,0 e 70,8 g, respectivamente, para o experimento 1 (tendo originado pintos de 41,0 e 49,0g de peso médio) e 66,9 e 75,1g (tendo originado pintos de 46,2 e 52,1g de peso médio), respectivamente, para o experimento 2. Uma parte destes ovos teve o peso de seus componentes determinado. À eclosão, 288 pintos, metade de cada categoria de peso de ovo, foram distribuídos em um delineamento inteiramente casualizado, em 48 parcelas. Cada parcela alojou seis pintos, metade de cada sexo. Os tratamentos experimentais obedeceram a esquema fatorial 2x3 (2 pesos de ovos x 3 tipos de rações préiniciais). Utilizaram-se três dietas de formulação comercial na fase pré-inicial: ração peletizada, com menor nível energético (2.900 kcal/kg), protéico e de aminoácidos, considerada uma dieta de baixos níveis nutricionais (PBN); ração farelada, de mesma composição nutricional da ração peletizada (FBN); ração farelada, com maior nível energético (2.950 kcal/kg), protéico e de aminoácidos, considerada uma dieta de altos níveis nutricionais (FAN). Foram avaliados o tempo de consumo das dietas pré-iniciais e, aos sete e 14 dias, o ganho de peso, o peso vivo, o consumo de ração e a conversão alimentar. Do término das rações pré-iniciais até os 14 dias de idade, as aves receberam uma mesma ração farelada. Em ambos os experimentos o albúmen foi mais pesado nos ovos classificados como pesados (P<0,05). No experimento 1, a dieta PBN foi consumida mais rapidamente por pintos oriundos de ovos pesados. Aos sete dias, a dieta PBN melhorou o peso vivo, o ganho de peso e a conversão alimentar (P<0,05). Já o peso do ovo influenciou o peso vivo, que foi maior para os pintos oriundos de ovos pesados (P<0,05). O benefício da dieta PBN quanto à conversão alimentar, se manteve aos 14 dias, e o peso vivo dos pintos oriundos de ovos pesados que receberam a dieta PBN foi maior do que aqueles que receberam as dietas FBN e FAN (P<0,05). No experimento 2, não houve diferença significativa para o tempo de consumo das dietas pré-iniciais (P>0,05). Aos sete dias, as dietas PBN e FAN proporcionaram aos pintos melhor conversão alimentar (P<0,05). Porém, aos 14 dias, não houve diferença significativa entre as variáveis (P>0,05). Concluiu-se que, em ovos de matrizes de mesma idade, a diferença no peso dos ovos deve-se ao incremento de albúmen. Quanto ao desempenho, os pintos oriundos de matrizes de idade intermediária foram mais influenciados pelas diferenças de peso dos ovos e composições das dietas, sendo que a dieta peletizada beneficiou o desempenho dos mesmos, mesmo apresentando menor nível nutricional. / Two experiments were conducted to evaluate different pre-starter diets on performance of chickens from light and heavy eggs hatched from Ross breeders of different ages. In the experiment 1, these eggs, that showed weight of 59,0 and 70,8g, were hatched from breeders of 42 weeks of age. In the experiment 2, these eggs, that showed 66,9 and 75,1g, were hatched from breeders of 60 weeks of age. The eggs percentage composition was evaluated. After hatching, 288 day-old chicks, males and females, were weight and allocated in 48 cages. In the experiment 1, their live weight was 41,0 and 49,0g; in the experiment 2, 46,2 e 52,1g. The performance trials were conducted in a completely randomized design with 6 treatments and 8 replicates, and the treatments consisted of a 2 3 factorial arrangement of egg weight (light and heavy) and three types of the pre-starter feed. The pre-starter diets used were: micro-pelleted diet, with 2900 kcal/kg and low levels of protein and amino acids (P); mash diet, with the same composition (M1); and mash diet, with 2950 kcal/kg and high protein. This feed was provided at a fixed amount of 200 g per chick and, after that, the birds in all treatments were fed a common starter diet in mash form, until 14 days of age. Live weight, weight gain, feed intake and feed conversion were evaluated on a pen basis. The time required for total consumption of the pre-starter feeds was monitored. In both experiments, the albumen was heavier than the other components. In experiment 1, there was influence of the egg weight only in the first week (P<0,05). At seven days, P diet improved the live weight, weight gain and feed conversion (P<0,05). At 14 days, P diet also improved feed conversion, as well the live weight of chicks from heavy eggs. In the same experiment, heavy chicks consumed 200 g of P diet faster than light chicks. In the experiment 2, the time of intake was not different among treatments (P>0,05). There was not influence of egg weight, but there was influence of P and F2 diets in the first week, that improved the feed conversion. However, this influence did not remain to 14 days (P>0,05). It was concluded that albumen is the heaviest component of egg, inside the same age of breeders. It was also concluded that the chicks from breeders of 42 weeks of age were more influenced by different pre-starter diet composition and egg weight than the chicks from breeders of 60 weeks of age. Although P diet showed lower nutritional level, it improved the performance of chicks from breeders of 42 weeks of age.

by Zhang Xiang. / "June 2003." / Thesis (Ph.D.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (p. 165-174). / Electronic reproduction. Hong Kong : Chinese University of Hong Kong, [2012] System requirements: Adobe Acrobat Reader. Available via World Wide Web. / Mode of access: World Wide Web. / Abstracts in English and Chinese.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-08-22T11:24:50Z No. of bitstreams: 1 2010 - Vin?cius Machado dos Santos.pdf: 995846 bytes, checksum: 696b6b0e2360cb9f5762b0890f9801b2 (MD5) / Made available in DSpace on 2018-08-22T11:24:50Z (GMT). No. of bitstreams: 1 2010 - Vin?cius Machado dos Santos.pdf: 995846 bytes, checksum: 696b6b0e2360cb9f5762b0890f9801b2 (MD5) Previous issue date: 2010-09-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / A study was carried out to evaluate the use of Saccharomyces cerevisiae cell wall (SCCW) as an anti-mycotoxin additive in broiler chicks? diets slaughtered at 22 day age on performance, carcass characteristics and organ weights. It was used a randomized complete block design with four treatments in a factorial 2 (with aflatoxin, without aflatoxin) x 2 (with adsorbent, without adsorbent). For the performance parameters evaluated, there was a significant difference only on feed intake, when the SCCW was added to the rations. There was no interaction between aflatoxin and SCCW factors in the diet on the performance variables studied. The addition of SCCW to the chicks? diets stimulated the feed intake during 1-21 days of age. However, that did not reflect a significant increase in weight gain, or a change in feed conversion. There was a significant interaction between factors aflatoxin and SCCW on the weight post-fasting, cold carcass weight and thigh+upper weight. For the absolute hot carcass weights, breast, back and wings and carcass yields and relative weights of the cuts it was not observed a significant interaction between factors. The growth performance of broilers in the period 1-21 days old was not influenced by the levels of aflatoxin and SCCW factors used in experimental diets. Nevertheless for body weight after fasting, the cold carcass weight and absolute weight of the thigh + drumstick, the SCCW acted as an anti-mycotoxin additive by minimizing the aflatoxin toxicity. / Objetivou-se com este experimento avaliar a utiliza??o da parede celular de Saccharomyces cerevisiae (PCSC) como aditivo antimicotoxina em ra??es destinadas a frangos de corte abatidos aos 22 dias de idade sobre o desempenho, caracter?sticas de carca?a e pesos de ?rg?os. Foi adotado um delineamento experimental em blocos casualizados, com 4 tratamentos dispostos em um esquema fatorial 2 (sem aflatoxina; com aflatoxina) x 2 (sem adsorvente; com adsorvente). Para os par?metros de desempenho avaliados, observou-se diferen?a significativa apenas sobre o consumo alimentar, quando a PCSC foi adicionada ?s ra??es. N?o houve intera??o entre os fatores aflatoxina e PCSC nas ra??es sobre as vari?veis de desempenho estudadas. A adi??o de PCSC ? ra??o dos frangos estimulou o consumo de ra??o no per?odo de 1 a 21 dias de idade, entretanto, isso n?o se refletiu em aumento significativo no ganho de peso dos frangos ou altera??o na convers?o alimentar. Houve intera??o significativa entre os fatores aflatoxina e PCSC sobre o peso vivo p?s-jejum, carca?a resfriada e o peso da coxa+sobrecoxa. Para os pesos absolutos de carca?a quente, peito, dorso e asas, rendimento das carca?as e pesos relativos dos cortes n?o se observou intera??o significativa entre os fatores estudados. O desempenho zoot?cnico de frangos de corte no per?odo de 1 a 21 dias de idade n?o foi influenciado pelos n?veis dos fatores aflatoxina e PCSC utilizados nas ra??es experimentais. J? para peso vivo p?s-jejum, peso da carca?a resfriada e peso absoluto da coxa+sobrecoxa, houve uma poss?vel atua??o da PCSC como aditivo antimicotoxina, ao minimizar a a??o t?xica da aflatoxina.

Elevated yolk progesterone has been shown to impair prenatal, but facilitate postnatal auditory learning in bobwhite quail chicks. Elevated yolk testosterone has facilitated prenatal learning, but its effects on postnatal auditory learning in quail are unknown. Either testosterone or an oil vehicle was injected into bobwhite quail eggs prior to incubation. Control eggs were unmanipulated. Following hatching, chicks were exposed to a conspecific maternal call (A or B) for 240 min. At 48 hr, chicks were tested for their preference for the familiarized vs. novel call. All groups demonstrated a preference for the familiar call (p < .05), but minimal between group difference were found. Contrary to previous research, elevated yolk testosterone neither facilitated nor impaired postnatal auditory learning in bobwhite quail chicks. Further research will examine underlying mechanisms responsible for differential effects and explore if similar systems are involved in other species-typical processes such as social motivation. / Includes bibliography. / Thesis (M.A.)--Florida Atlantic University, 2017. / FAU Electronic Theses and Dissertations Collection

1,25-dihydroxyvitamin D3 (1,25(OH)2D3) has been shown to mediate the rapid, non-nuclear stimulation of calcium and phosphate transport in chick intestine through binding to a receptor localized in the basal lateral membrane. By using an antibody to the N-terminus of the membrane receptor, studies were undertaken to determine whether a comparable protein exists in kidney and brain, and whether it is present in a particular subcellular fraction. The first step was to establish fractionation protocols to separate subcellular organelles as judged by marker enzyme analyses. Differential centrifugation and Percoll gradient fractions were prepared from chick kidney and brain whole homogenates by two methods (method 1 and method 2). Protein and marker enzymes were analyzed in each fraction to determine the distribution of organelles. By method 1, the organelles were not adequately separated. By method 2, chick kidney and brain were found to have the same order of organelle distribution: In the post-nuclear pellet (P2), fraction 1 was found to be enriched for the lysosomal marker acid phosphatase; fractions 2-5 were found to be enriched for the mitochondrial marker succinate dehydrogenase; fraction 8 was found to be enriched for the Golgi marker α-D-mannosidase; and fraction 9 was found to be enriched for the plasma membrane marker Na+,K+ ATPase. In Percoll gradients of microsomal membranes prepared from the 100,000xg pellet (P3), fractions 1-3 contained the endoplasmic reticulum marker enzyme activity glucose-6-phosphatase. Subsequently, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE) and Western blots were performed using the antibody to 1,25(OH)2D3 receptors in chick intestinal basal lateral membrane. The areas of the bands were scanned by computer, and analyzed quantitatively. After establishing a suitable protein concentration for Western analysis, differential centrifugation and Percoll gradient fractions were analyzed. Finally, the specific binding of [3H]1,25(OH)2D3 was determined in Percoll gradient fractions to assess whether the receptor is functional. Plasma membrane 1,25(OH)2D3 receptors were found in both chick kidney and brain cells. Golgi membranes also were found to have receptor activity, perhaps since this organelle packages proteins for delivery to other membranes. In kidney, fraction P27 demonstrated a very high receptor activity, and [3H]1,25(OH)2D3 specific binding assays showed these membrane receptors are functional. Although this fraction lacks traditional marker enzyme activity, it may contain endocytic vesicles. The physiological function and the mechanism of action of plasma membrane receptors in these two tissues remain to be determined.

No description available.

The objectives of these experiments were to evaluate the main effects and interactive effects between dietary supplementation of a mannan oligosaccharide based product, Actigen® (ACT), and different levels of threonine in monogastrics, as evidenced by growth parameters, immune function, and intestinal health characteristics. In nursery piglets, ACT supplementation decreased average daily feed intake (P=0.04), but had no effect on body weight or feed conversion ratio. There were no noted differences between threonine levels on performance traits. There were no consistent differences in complete blood count or cytokine gene expression profiles in the blood. The highest level of threonine, 77% true ileal digestible threonine:lysine (Thr:Lys), increased villus height (P=0.007) and villus height:crypt depth (P=0.01). The lowest level, 57% Thr:Lys, decreased villus surface area (P=0.04) and goblet cell density (P=0.04). Supplementation with ACT increased total goblet cell area (P=0.02) and density (P=0.05). There were no interactions observed between ACT and Thr:Lys levels. In broiler chicks, feeding a diet containing 0.56% threonine decreased body weight (PPPP=0.07). On d 7, birds supplemented with ACT had heavier spleens as a percentage of body weight (P=0.01) compared to no ACT. When adjusted for body weight, the 0.56% threonine fed birds had smaller spleens (P=0.05) on d 7 when compared to the other threonine levels. Humerus (PPP=0.04) and birds fed 0.56% threonine had a higher concentration of potassium (PP=0.03) and few goblet cells (P=0.04) on d 7 when compared to the other threonine levels. In d 21 jejunum, supplementation with ACT reduced apical width (P=0.03) and surface area (P=0.02). An interaction was observed between ACT and threonine level in the jejunum on d 21 on basal width (P=0.03) and surface area (P=0.02), indicating that in diets lacking ACT, excess threonine increased villus size. Overall, ACT and threonine acted primarily independently to modulate the intestinal architecture of both nursery piglets and broiler chicks. However, in broiler chicks ACT and threonine interacted to alter villus size. These results indicate that ACT and threonine have direct effects on the intestines of monogastrics.

Destaca-se a Salmonella como um dos mais importantes patógenos veiculados por alimentos, devido ao fato de estar amplamente distribuído na natureza, possuir um grande número de reservatórios, apresentar sorotipos inespecíficos quanto aos hospedeiros e apresentar cepas multiresistentes aos antimicrobianos. Salmonella Enteritidis tornou-se um grande problema de saúde pública no Brasil, a partir da década de 80, quando foi associada a produtos avícolas. Desde 1962, a Salmonella Heidelberg tem sido isolada de aves e produtos derivados e aves no Brasil e tem reconhecida importância para saúde humana e em poedeiras comerciais na América do Norte. O presente trabalho teve por objetivo o estudo de diferentes cepas de Salmonella Heidelberg (SH) em comparação com Salmonella Enteritidis (SE) em pintinhos e frangos de corte no que diz respeito à capacidade de invasibilidade de órgãos, persistência de excreção fecal e alterações na morfometria intestinal. Para o estudo in vivo, 65 isolados de SH foram avaliados quanto à presença dos genes de virulência invA, lpfA e agfA e diferenciados por eletroforese em campo pulsado (PFGE). Outro objetivo foi testar uma mistura de ácidos orgânicos e óleos essenciais no controle de excreção fecal de SE. Duas amostras de SH não relacionadas pelo método de PFGE (SH23 e SH35) e uma cepa de SE padrão foram selecionadas para a inoculação das aves. As três cepas promoveram alteração na medida de vilosidades e criptas dos pintinhos, frente a grupo controle. A cepa SH35 promoveu maior reação inflamatória nos cecos. Ambas as cepas testadas foram capaz de atingir o fígado de pintinhos às 6 horas pós-inoculação. Tanto a SE como a SH demonstram persistência e intermitência de excreção fecal em frangos de corte até os 21dias pós-inoculação. A mistura de ácidos e óleos essenciais foi eficaz na redução da excreção fecal de SE, porém somente com o uso contínuo da mistura na ração. As cepas apresentaram comportamentos diferentes quando inoculadas em frangos de corte e devido à persistência destas nos intestinos dos frangos, prejuízo para as aves podem ocorrer, enquanto a subseqüente contaminação de carcaças, representa risco para a saúde humana. / Salmonella, one of the most important food borne pathogen due to the widely distribution in nature, large number of reservoirs, to show unspecific host serotypes and provide multiresistant strains to antibiotics. Salmonella Enteritidis became a big public health problem in Brasil, since 80’s, when it was correlated with poultry products. Since 1962, Salmonella Heidelberg has been isolated from birds and poultry products in Brazil, with importance in human health and layers in North America. The present work aimed to the study of different strains of Salmonella Heidelberg (SH) and compared with Salmonella Enteritidis (SE) in chicks and broiler chickens with regard to the ability of invasibilidade of organs, persistence of fecal excretion and changes in intestinal morphometry. For the in vivo study, 65 isolates of SH were evaluated for the presence of virulence genes invA, lpfA and agfA and differentiated by pulsed field gel electrophoresis (PFGE). Another objective was to test a organic acids and essential oils mixture for controlling SE fecal excretion. Two not related SH samples by the method of PFGE (SH23 and SH35) and a standard strain of SE were selected to the poultry inoculation. The three strains promoted changes in the measures of villi and crypts of the chicks intestine, when compared to the control group. The SH35strain promoted greater inflammatory reaction in the caeca. Both strains tested were able to achieve the chicks liver at 6 hours post inoculation. Both strains, SE and SH, showed persistence and intermittence of fecal excretion in broiler chickens until the 21days post-infection. The organic acids and essential oils mix was effective in reducing the SE fecal excretion, but only with the continuous use of the mix added to the feed. The strains showed different behavior when inoculated in broiler chickens, and because of the persistence of these strains in the chickens intestines, injury to birds may occur, while the subsequent carcasses contamination represents risk to human health.

Destaca-se a Salmonella como um dos mais importantes patógenos veiculados por alimentos, devido ao fato de estar amplamente distribuído na natureza, possuir um grande número de reservatórios, apresentar sorotipos inespecíficos quanto aos hospedeiros e apresentar cepas multiresistentes aos antimicrobianos. Salmonella Enteritidis tornou-se um grande problema de saúde pública no Brasil, a partir da década de 80, quando foi associada a produtos avícolas. Desde 1962, a Salmonella Heidelberg tem sido isolada de aves e produtos derivados e aves no Brasil e tem reconhecida importância para saúde humana e em poedeiras comerciais na América do Norte. O presente trabalho teve por objetivo o estudo de diferentes cepas de Salmonella Heidelberg (SH) em comparação com Salmonella Enteritidis (SE) em pintinhos e frangos de corte no que diz respeito à capacidade de invasibilidade de órgãos, persistência de excreção fecal e alterações na morfometria intestinal. Para o estudo in vivo, 65 isolados de SH foram avaliados quanto à presença dos genes de virulência invA, lpfA e agfA e diferenciados por eletroforese em campo pulsado (PFGE). Outro objetivo foi testar uma mistura de ácidos orgânicos e óleos essenciais no controle de excreção fecal de SE. Duas amostras de SH não relacionadas pelo método de PFGE (SH23 e SH35) e uma cepa de SE padrão foram selecionadas para a inoculação das aves. As três cepas promoveram alteração na medida de vilosidades e criptas dos pintinhos, frente a grupo controle. A cepa SH35 promoveu maior reação inflamatória nos cecos. Ambas as cepas testadas foram capaz de atingir o fígado de pintinhos às 6 horas pós-inoculação. Tanto a SE como a SH demonstram persistência e intermitência de excreção fecal em frangos de corte até os 21dias pós-inoculação. A mistura de ácidos e óleos essenciais foi eficaz na redução da excreção fecal de SE, porém somente com o uso contínuo da mistura na ração. As cepas apresentaram comportamentos diferentes quando inoculadas em frangos de corte e devido à persistência destas nos intestinos dos frangos, prejuízo para as aves podem ocorrer, enquanto a subseqüente contaminação de carcaças, representa risco para a saúde humana. / Salmonella, one of the most important food borne pathogen due to the widely distribution in nature, large number of reservoirs, to show unspecific host serotypes and provide multiresistant strains to antibiotics. Salmonella Enteritidis became a big public health problem in Brasil, since 80’s, when it was correlated with poultry products. Since 1962, Salmonella Heidelberg has been isolated from birds and poultry products in Brazil, with importance in human health and layers in North America. The present work aimed to the study of different strains of Salmonella Heidelberg (SH) and compared with Salmonella Enteritidis (SE) in chicks and broiler chickens with regard to the ability of invasibilidade of organs, persistence of fecal excretion and changes in intestinal morphometry. For the in vivo study, 65 isolates of SH were evaluated for the presence of virulence genes invA, lpfA and agfA and differentiated by pulsed field gel electrophoresis (PFGE). Another objective was to test a organic acids and essential oils mixture for controlling SE fecal excretion. Two not related SH samples by the method of PFGE (SH23 and SH35) and a standard strain of SE were selected to the poultry inoculation. The three strains promoted changes in the measures of villi and crypts of the chicks intestine, when compared to the control group. The SH35strain promoted greater inflammatory reaction in the caeca. Both strains tested were able to achieve the chicks liver at 6 hours post inoculation. Both strains, SE and SH, showed persistence and intermittence of fecal excretion in broiler chickens until the 21days post-infection. The organic acids and essential oils mix was effective in reducing the SE fecal excretion, but only with the continuous use of the mix added to the feed. The strains showed different behavior when inoculated in broiler chickens, and because of the persistence of these strains in the chickens intestines, injury to birds may occur, while the subsequent carcasses contamination represents risk to human health.

Orientador: Isabel Cristina Boleli / Banca: Renato Luis Furlan / Banca: Luciana Thie Seki Dias / Resumo: Foram avaliados os parâmetros hematológicos de pintos submetidos à inoculação de microbiota intestinal de aves adultas poedeiras e de corte. Para isso, foi feito um experimento para testar o efeito da técnica de inoculação via esofágica. E este não afetou os parâmetros hematológicos. Num segundo experimento, foram utilizados pintos machos recém eclodidos (linhagem COBB). O delineamento experimental foi o inteiramente casualizado, seguindo fatorial 3 x 4, sendo 3 tratamentos [sem inoculação (Controle), inoculação de microbiota intestinal de frangos de corte (IMIFC), inoculação de microbiota intestinal de aves de postura adultas criadas em gaiola (IMIP)] e 4 períodos (1º, 3º, 5º e 7º dia pós-inoculação). A cada período, cinco aves por tratamento foram pesadas e sacrificadas para análise dos seguintes parâmetros: contagem total de eritrócitos (RBC, x106/mm3), hemoglobina (HGB, g/dL), hematócrito (HCT, %), volume corpuscular médio (MCV, μm3), contagem total e diferencial de leucócitos e relação heterófilo:linfócito. Os dados foram submetidos à análise de variância e as médias comparadas pelo Teste de Tukey. Houve resposta temporária dos pintos que receberam inóculo independente do tipo de microbiota. Essa resposta foi observada através do aumento do RBC, HCT e HGB que durou até o 3º dia. Não ocorreu depressão do sistema imune, apenas variação da porcentagem de heterófilos e linfócitos na circulação. / Abstract: Two experiments were conducted to determine if the technique of esophageal inoculation (Experiment I) and the esophageal inoculation of intestinal microbiota evaluated of adult birds influence the haematological parameters of male broiler chicks. In experiment I, technique of inoculation had no significant effects on haematological parameters [erythrocytes (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), counting total and differential leukocyte and the rate heterophil: lymphocyte]. In experiment II, the experimental design was entirely randomly, following factorial 3 x 4, [3 treatments: no inoculation (Control), inoculation of intestinal microbiota of broiler chickens (IIMBC), inoculation of intestinal microbiota of layer hens (IIMLH); 4 periods: 1, 3, 5 and 7 days post-inoculation]. Inoculation of intestinal microbiota caused a increase in the RBC, HCT and HGB already in the 1 st day post-inoculation. This effect remained until the 3 rd day, but only in the chicks of the treatment IIMBC. In the 3 rd day, an increase in the heterophil percentage and a reduction in the lymphocyte percentage were also observed. The results showed that inoculation of intestinal microbiota caused an immediate and temporary heamatological response. / Mestre