Made available in DSpace on 2014-06-11T19:30:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-25Bitstream added on 2014-06-13T21:00:40Z : No. of bitstreams: 1 pires_dl_me_jabo.pdf: 2181256 bytes, checksum: 1c7c0de488fc5a788e922d6932500dc1 (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Foram avaliados os parâmetros hematológicos de pintos submetidos à inoculação de microbiota intestinal de aves adultas poedeiras e de corte. Para isso, foi feito um experimento para testar o efeito da técnica de inoculação via esofágica. E este não afetou os parâmetros hematológicos. Num segundo experimento, foram utilizados pintos machos recém eclodidos (linhagem COBB). O delineamento experimental foi o inteiramente casualizado, seguindo fatorial 3 x 4, sendo 3 tratamentos [sem inoculação (Controle), inoculação de microbiota intestinal de frangos de corte (IMIFC), inoculação de microbiota intestinal de aves de postura adultas criadas em gaiola (IMIP)] e 4 períodos (1º, 3º, 5º e 7º dia pós-inoculação). A cada período, cinco aves por tratamento foram pesadas e sacrificadas para análise dos seguintes parâmetros: contagem total de eritrócitos (RBC, x106/mm3), hemoglobina (HGB, g/dL), hematócrito (HCT, %), volume corpuscular médio (MCV, μm3), contagem total e diferencial de leucócitos e relação heterófilo:linfócito. Os dados foram submetidos à análise de variância e as médias comparadas pelo Teste de Tukey. Houve resposta temporária dos pintos que receberam inóculo independente do tipo de microbiota. Essa resposta foi observada através do aumento do RBC, HCT e HGB que durou até o 3º dia. Não ocorreu depressão do sistema imune, apenas variação da porcentagem de heterófilos e linfócitos na circulação. / Two experiments were conducted to determine if the technique of esophageal inoculation (Experiment I) and the esophageal inoculation of intestinal microbiota evaluated of adult birds influence the haematological parameters of male broiler chicks. In experiment I, technique of inoculation had no significant effects on haematological parameters [erythrocytes (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), counting total and differential leukocyte and the rate heterophil: lymphocyte]. In experiment II, the experimental design was entirely randomly, following factorial 3 x 4, [3 treatments: no inoculation (Control), inoculation of intestinal microbiota of broiler chickens (IIMBC), inoculation of intestinal microbiota of layer hens (IIMLH); 4 periods: 1, 3, 5 and 7 days post-inoculation]. Inoculation of intestinal microbiota caused a increase in the RBC, HCT and HGB already in the 1 st day post-inoculation. This effect remained until the 3 rd day, but only in the chicks of the treatment IIMBC. In the 3 rd day, an increase in the heterophil percentage and a reduction in the lymphocyte percentage were also observed. The results showed that inoculation of intestinal microbiota caused an immediate and temporary heamatological response.

Destaca-se a Salmonella como um dos mais importantes patógenos veiculados por alimentos, devido ao fato de estar amplamente distribuído na natureza, possuir um grande número de reservatórios, apresentar sorotipos inespecíficos quanto aos hospedeiros e apresentar cepas multiresistentes aos antimicrobianos. Salmonella Enteritidis tornou-se um grande problema de saúde pública no Brasil, a partir da década de 80, quando foi associada a produtos avícolas. Desde 1962, a Salmonella Heidelberg tem sido isolada de aves e produtos derivados e aves no Brasil e tem reconhecida importância para saúde humana e em poedeiras comerciais na América do Norte. O presente trabalho teve por objetivo o estudo de diferentes cepas de Salmonella Heidelberg (SH) em comparação com Salmonella Enteritidis (SE) em pintinhos e frangos de corte no que diz respeito à capacidade de invasibilidade de órgãos, persistência de excreção fecal e alterações na morfometria intestinal. Para o estudo in vivo, 65 isolados de SH foram avaliados quanto à presença dos genes de virulência invA, lpfA e agfA e diferenciados por eletroforese em campo pulsado (PFGE). Outro objetivo foi testar uma mistura de ácidos orgânicos e óleos essenciais no controle de excreção fecal de SE. Duas amostras de SH não relacionadas pelo método de PFGE (SH23 e SH35) e uma cepa de SE padrão foram selecionadas para a inoculação das aves. As três cepas promoveram alteração na medida de vilosidades e criptas dos pintinhos, frente a grupo controle. A cepa SH35 promoveu maior reação inflamatória nos cecos. Ambas as cepas testadas foram capaz de atingir o fígado de pintinhos às 6 horas pós-inoculação. Tanto a SE como a SH demonstram persistência e intermitência de excreção fecal em frangos de corte até os 21dias pós-inoculação. A mistura de ácidos e óleos essenciais foi eficaz na redução da excreção fecal de SE, porém somente com o uso contínuo da mistura na ração. As cepas apresentaram comportamentos diferentes quando inoculadas em frangos de corte e devido à persistência destas nos intestinos dos frangos, prejuízo para as aves podem ocorrer, enquanto a subseqüente contaminação de carcaças, representa risco para a saúde humana. / Salmonella, one of the most important food borne pathogen due to the widely distribution in nature, large number of reservoirs, to show unspecific host serotypes and provide multiresistant strains to antibiotics. Salmonella Enteritidis became a big public health problem in Brasil, since 80’s, when it was correlated with poultry products. Since 1962, Salmonella Heidelberg has been isolated from birds and poultry products in Brazil, with importance in human health and layers in North America. The present work aimed to the study of different strains of Salmonella Heidelberg (SH) and compared with Salmonella Enteritidis (SE) in chicks and broiler chickens with regard to the ability of invasibilidade of organs, persistence of fecal excretion and changes in intestinal morphometry. For the in vivo study, 65 isolates of SH were evaluated for the presence of virulence genes invA, lpfA and agfA and differentiated by pulsed field gel electrophoresis (PFGE). Another objective was to test a organic acids and essential oils mixture for controlling SE fecal excretion. Two not related SH samples by the method of PFGE (SH23 and SH35) and a standard strain of SE were selected to the poultry inoculation. The three strains promoted changes in the measures of villi and crypts of the chicks intestine, when compared to the control group. The SH35strain promoted greater inflammatory reaction in the caeca. Both strains tested were able to achieve the chicks liver at 6 hours post inoculation. Both strains, SE and SH, showed persistence and intermittence of fecal excretion in broiler chickens until the 21days post-infection. The organic acids and essential oils mix was effective in reducing the SE fecal excretion, but only with the continuous use of the mix added to the feed. The strains showed different behavior when inoculated in broiler chickens, and because of the persistence of these strains in the chickens intestines, injury to birds may occur, while the subsequent carcasses contamination represents risk to human health.

Dois experimentos foram conduzidos de forma semelhante, variando apenas a idade do lote de matrizes Ross que originou os ovos (42 e 60 semanas de idade, respectivamente, para os experimentos 1 e 2), com o objetivo de avaliar o desempenho de pintos oriundos de ovos leves e pesados, alimentados com dietas pré-iniciais de diferentes formas físicas e níveis nutricionais.. Foram incubados ovos apresentando peso médio de 59,0 e 70,8 g, respectivamente, para o experimento 1 (tendo originado pintos de 41,0 e 49,0g de peso médio) e 66,9 e 75,1g (tendo originado pintos de 46,2 e 52,1g de peso médio), respectivamente, para o experimento 2. Uma parte destes ovos teve o peso de seus componentes determinado. À eclosão, 288 pintos, metade de cada categoria de peso de ovo, foram distribuídos em um delineamento inteiramente casualizado, em 48 parcelas. Cada parcela alojou seis pintos, metade de cada sexo. Os tratamentos experimentais obedeceram a esquema fatorial 2x3 (2 pesos de ovos x 3 tipos de rações préiniciais). Utilizaram-se três dietas de formulação comercial na fase pré-inicial: ração peletizada, com menor nível energético (2.900 kcal/kg), protéico e de aminoácidos, considerada uma dieta de baixos níveis nutricionais (PBN); ração farelada, de mesma composição nutricional da ração peletizada (FBN); ração farelada, com maior nível energético (2.950 kcal/kg), protéico e de aminoácidos, considerada uma dieta de altos níveis nutricionais (FAN). Foram avaliados o tempo de consumo das dietas pré-iniciais e, aos sete e 14 dias, o ganho de peso, o peso vivo, o consumo de ração e a conversão alimentar. Do término das rações pré-iniciais até os 14 dias de idade, as aves receberam uma mesma ração farelada. Em ambos os experimentos o albúmen foi mais pesado nos ovos classificados como pesados (P<0,05). No experimento 1, a dieta PBN foi consumida mais rapidamente por pintos oriundos de ovos pesados. Aos sete dias, a dieta PBN melhorou o peso vivo, o ganho de peso e a conversão alimentar (P<0,05). Já o peso do ovo influenciou o peso vivo, que foi maior para os pintos oriundos de ovos pesados (P<0,05). O benefício da dieta PBN quanto à conversão alimentar, se manteve aos 14 dias, e o peso vivo dos pintos oriundos de ovos pesados que receberam a dieta PBN foi maior do que aqueles que receberam as dietas FBN e FAN (P<0,05). No experimento 2, não houve diferença significativa para o tempo de consumo das dietas pré-iniciais (P>0,05). Aos sete dias, as dietas PBN e FAN proporcionaram aos pintos melhor conversão alimentar (P<0,05). Porém, aos 14 dias, não houve diferença significativa entre as variáveis (P>0,05). Concluiu-se que, em ovos de matrizes de mesma idade, a diferença no peso dos ovos deve-se ao incremento de albúmen. Quanto ao desempenho, os pintos oriundos de matrizes de idade intermediária foram mais influenciados pelas diferenças de peso dos ovos e composições das dietas, sendo que a dieta peletizada beneficiou o desempenho dos mesmos, mesmo apresentando menor nível nutricional. / Two experiments were conducted to evaluate different pre-starter diets on performance of chickens from light and heavy eggs hatched from Ross breeders of different ages. In the experiment 1, these eggs, that showed weight of 59,0 and 70,8g, were hatched from breeders of 42 weeks of age. In the experiment 2, these eggs, that showed 66,9 and 75,1g, were hatched from breeders of 60 weeks of age. The eggs percentage composition was evaluated. After hatching, 288 day-old chicks, males and females, were weight and allocated in 48 cages. In the experiment 1, their live weight was 41,0 and 49,0g; in the experiment 2, 46,2 e 52,1g. The performance trials were conducted in a completely randomized design with 6 treatments and 8 replicates, and the treatments consisted of a 2 3 factorial arrangement of egg weight (light and heavy) and three types of the pre-starter feed. The pre-starter diets used were: micro-pelleted diet, with 2900 kcal/kg and low levels of protein and amino acids (P); mash diet, with the same composition (M1); and mash diet, with 2950 kcal/kg and high protein. This feed was provided at a fixed amount of 200 g per chick and, after that, the birds in all treatments were fed a common starter diet in mash form, until 14 days of age. Live weight, weight gain, feed intake and feed conversion were evaluated on a pen basis. The time required for total consumption of the pre-starter feeds was monitored. In both experiments, the albumen was heavier than the other components. In experiment 1, there was influence of the egg weight only in the first week (P<0,05). At seven days, P diet improved the live weight, weight gain and feed conversion (P<0,05). At 14 days, P diet also improved feed conversion, as well the live weight of chicks from heavy eggs. In the same experiment, heavy chicks consumed 200 g of P diet faster than light chicks. In the experiment 2, the time of intake was not different among treatments (P>0,05). There was not influence of egg weight, but there was influence of P and F2 diets in the first week, that improved the feed conversion. However, this influence did not remain to 14 days (P>0,05). It was concluded that albumen is the heaviest component of egg, inside the same age of breeders. It was also concluded that the chicks from breeders of 42 weeks of age were more influenced by different pre-starter diet composition and egg weight than the chicks from breeders of 60 weeks of age. Although P diet showed lower nutritional level, it improved the performance of chicks from breeders of 42 weeks of age.

The steroid hormone 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] rapidly stimulates the uptake of phosphate in isolated chick intestinal cells, while the steroid 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] inhibits the rapid stimulation by 1,25(OH)2D3. Previous work in this laboratory has indicated that 24,25(OH)2D3 inhibits phosphate uptake in isolated intestinal cells and perfused duodenal loops. It is critical to show this effect in the whole animal to determine the presence of any confounding factors. Studies were therefore undertaken to determine if 24,25(OH)2D3 had a similar effect in vivo. 24,25(OH)2D3 has two isomers which are 24R, 25dihydroxyvitamin D3 [24R,25(OH)2D3] and 24S, 25dihydroxyvitamin D3 [24S,25(OH)2D3]. We studied these two isomers separately and tested them over a time course of 1, 5, 10, 15, and 18 h after steroid using chicks on regular diet, but fasted, and chicks on a lower vitamin D diet. All chicks were anesthetized prior to surgical exposure of the duodenal loop and injection of a solution containing H3 32PO4 into the lumen. An initial time course study of phosphate transport determined that 3 to 9 min of absorption in vivo was in a linear range, as judged by serum levels of radioactivity. Chicks were then injected with either 200 μg of 24R,25(OH)2D3, 20 μg of 24S,25(OH)2D3, or vehicle for control groups within the same time course studies. We found that the isomers had different effects on phosphate absorption. 24R,25(OH)2D3 had a hypophosphatemic effect in vivo. The serum levels of radionuclide revealed hypophosphatemic effects at 1, 5, 15, and 18 h time points with a decrease of 20%, 42%, 39%, and 43%, respectively, (P< 0.05) compared with controls; chicks raised on a low vitamin D diet also showed a decrease in phosphate absorption at 10 h time point by 33%. In contrast, 24S,25(OH)2D3 stimulated intestinal phosphate absorption at the 5-h time point by 64%, but had no other effects at the other time points tested. Because 24S,25(OH)2D3 was largely ineffective, dose-response studies were undertaken with only the 24R,25(OH)2D3 isomer. In comparing phosphate absorption in chicks fasted 18 h, and dosed with vehicle, 100 μg, 200 μg, or 300 μg of steroid 1 h prior to experimentation it was found that the lowest dose increased absorption to 99% of controls, while the 200 μg and 300 μg doses decreased phosphate absorption. (48 pages)

Thesis (MScAgric)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: The South African Ostrich industry was established more than 100 years ago. Despite appreciable fluctuations and modifications in demand, the industry perseveres, with meat and leather as the current primary driving force. Low and variable early chick survival is globally considered as a major constraint in the industry. In spite of obvious welfare and production implications, research findings concerning ostrich chick survival are scant. Four studies involving chick survival to 24 weeks post hatch were thus performed. South African Black ostrich (Struthio camelus var. domesticus) data obtained from a commercial pair-bred ostrich flock maintained at the Oudtshoorn Experimental Farm, South Africa comprising 10418 records were utilized to run a series of single- and multiple-trait binomial analyses using either linear models (ASREML) or threshold models (Monte Carlo Markov Chain methods, employing Gibbs sampling software) respectively. Suitable fixed and random effect models together with variance and (co)variance components were derived from these analyses. Ostrich chick survival to 24 weeks post hatch was low (28%) with a large environmental component. Preincubation storage time only affected 0 to 3 week survival, while incubator had a significant effect on cumulative survival traits to 24 weeks as well as on 13 to 24 week survival. Female chicks outperformed males at 0 to 12 weeks, 0 to 24 weeks, 4 to 12 weeks and 13 to 24 weeks with regard to survival. Hen age was significant for all traits except survival during the first week as well as for survival from 13 to 24 weeks post hatch. All analyses exhibited comparable low heritability estimates for all survival traits except for 0 to 1 week survival and 13 to 24 week survival (h2 = 0.12 and 0.10; m2 = 0.08 and 0.07 respectively) which demonstrated higher additive genetic and maternal components when threshold models, using Gibbs sampling algorithms, were employed. Neither moisture loss nor day of external pipping exhibited notable additive genetic correlations with any of the estimated survival traits. Day-old chick weight demonstrated a low correlation with early chick survival (rg = 0.24 ± 0.19) with heavier chicks tending to have a higher survival probability. The effects of rearing method on early chick survival and growth were also investigated. Comparisons between chicks reared and fostered by adult ostriches in a semiextensive environment and chicks imprinted onto humans under an intensive system and between regular human handling in an intensive rearing system and the standard farm protocol of an intensive rearing system were made. No difference in the early chick survival to 3 weeks post hatch of chicks reared by foster parents and those chicks imprinted onto humans was demonstrated. At later ages, those chicks reared by foster parents outperformed human imprinted chicks. Human imprinted chicks exposed to regular handling demonstrate an enhanced early survival when compared to chicks exposed to conventional rearing protocol in intensive systems. The study is seen to provide guidance for further research on this topic. / AFRIKAANSE OPSOMMING: Die Suid-Afrikaanse volstruisbedryf is meer as 100 jaar gelede gevestig. Die bedryf is volhoubaar, ten spyte van ‘n fluktuerende aanvraag na volstruisprodukte. Vleis en leer is tans die produkte wat die meeste tot die inkomste van boere bydra. Lae en wisselende kuikenoorlewing is ‘n belangrike knelpunt in die bedryf. Navorsingsuitsette rakende kuikenoorlewing is skaars, ten spyte van ooglopende welsyns- en produksie implikasies. Kuikenoorlewing tot en met 24 weke na uitbroei is dus in vier afsondelike studies ondersoek. Data van 10418 kuikens uit ‘n kommersiële broeipaargeteelde Suid-Afrikaanse Swart (Struthio camelus var. domesticus) kudde op die Oudtshoorn Navorsingsplaas, Suid-Afrika is gebruik om enkel- en meervoudige eienskappe met of liniêre modelle (ASREML) of drempelwaarde modelle (Monte Carlo Markov Ketting metodes, met Gibbs monstering sagteware) te pas. Die metodes is gebruik om toepaslike vaste- en toevalseffekte modelle saam met (ko)variansie komponente te bekom. Kuikenoorlewing tot 24 weke na uitbroei was laag (28%), met ‘n groot omgewingsbydraende komponent. Stoor van eiers voor pak het slegs kuikenoorlewing van 0 tot 3 weke beïnvloed, terwyl die broeikas wat gebruik is ‘n betekenisvolle invloed op kuikenoorlewing tot 24 weke ouderdom, sowel as op -oorlewing van 13 tot 24 weke gehad het. Wyfiekuikens het beter oorleef as mannetjie kuikens vir oorlewing gemeet van 0 tot 12 weke, 0 tot 24 weke, 4 tot 12 weke en van 13 tot 24 weke. Broeiwyfie ouderdom het ‘n betekenisvolle invloed op kuikenoorlewing in die eerste week na uitbroei en van 13 tot 24 weke na uitbroei, gehad. Kuikenoorlewing was relatief laag oorerflik, behalwe vir oorlewing van 0 tot 1 week en vir oorlewing van 13 tot 24 weke van ouderdom (h2 = 0.12 en 0.10; m2 = 0.08 en 0.07 onderskeidelik). Drempelwaarde modelle met Gibbs monstering algoritmes het hoër additiewe en maternale variansie verhoudings in vergelyking met liniêre modelle aangedui. Genetiese korrelasies van vogverlies en die dag van uitwendige pik met kuikenoorlewing was oor die algemeen onbeduidend. Dagoudkuikengewig is laag gekorreleerd met vroeë kuikenoorlewing (rg = 0.24 ± 0.19), met swaarder kuikens wat oor die algemeen beter oorleef. Die gevolge van verskillende grootmaak metodes op vroeë kuikenoorlewing en -groei is ook ondersoek. Vergelykings tussen kuikens grootgemaak deur volwasse volstruise as pleegouers in ‘n semi-intensiewe omgewing en kuikens grootgemaak deur mense onder ‘n intensiewe stelsel en tussen kuikens grootgemaak deur gewone menslike hantering in ‘n intensiewe grootmaak sisteem onder standard plaas protokol was bestudeer. Geen verskille was gevind in vroeë kuikenoorlewing tot 3 weke na uitbroei, van die kuikens grootgemaak deur volwasse volstruise en tussen die kuikens grootgemaak deur mense . By latere ouderdomme, het die kuikens wat grootgemaak was deur volwasse volstruise beter oorlewing getoon as die kuikens grootgemaak deur mense. Kuikens grootgemaak deur mense wat blootgestel was aan gereelde hantering het verhoogde vroeë oorlewing getoon in vergelyking met kuikens grootgemaak deur konvensionele grootmaak protokol in intensiewe stelsels. Die studie kan dus beskou word om leiding te verskaf vir verdere navorsing oor hierdie onderwerp.

Filial imprinting is a type of early developmental learning in which certain species buildstrong and mostly irreversible connections to objects or individuals. These connections couldbe shaped by the contribution of several stimuli including the fragments of auditory and visual components. This study aims to describe the duration of the imprinting process as well asestimate long-term behavioural changes in chicks. In this experiment, a total of 78 chicks were used out of which 39 were exposed to imprinting stimuli and the rest served as control.We applied three different tests and replicated them after the imprinting procedure. These tests are Imprinting Preference Test, Social Preference Test and Social isolation Test. Imprinted chicks had a constantly shorter latency to approach the imprinting stimuli for boththose two experiments with or without novel objects, whereas no preferences spent time inimprinting stimuli. However, introducing a novel object affected imprinting preferences more in terms of spending a longer time around the hen zone. During social isolation, the control group showed a relatively higher rate of distress calls even though our results don’t bear on the significant effect of filial imprinting on changes in distress calling. Overall, this study suggests the presence of long-lasting filial imprinting that is more triggered by external situations.

Thesis (MPhil (Animal Sciences))--University of Stellenbosch, 2007. / ENGLISH ABSTRACT: Over the last few decades various authors have reported the influence of day old chick quality on integrated broiler industries. Although various methods of determining chick quality have been reported, defining a good or a first grade day old chick can be problematic as it involves many subjective measurements. Incubation temperature (embryo temperature) is probably the biggest, most influential factor during incubation on chick quality. High temperatures cause the most damage. It leads to poor growth, stress, black buttons, threads, weak chicks, chicks with poorly erupted down that are bleached in appearance, low hatchability, late embryonic death and early broiler mortality. This trial was designed to test whether 37.2, 37.4 or 37.5ºC was the optimum incubation temperature for hatching Hybro G+ chicks with superior quality. The parameters that were measured were the chick length, bodyweight, and the yolk residue of the day old chicks of the flocks set at different setter temperatures. After hatching, 1 440 chicks (480 chicks per temperature treatment) were placed at a broiler facility where the daily mortalities, weekly bodyweight gain and feed conversion were recorded and calculated. The age of the breeder flock had a significant effect on all three parameters measured in the incubation trial with p values of <0.001, <0.001 and 0.005 respectively. There were no significant differences in chick quality parameters due to different incubation temperatures. The age x temperature interaction could be attributed to the large influence of the age of the breeder on egg size and consequently chick parameters. The results could be related to the fact that bigger eggs from older breeder flocks have higher initial egg mass, which will result in heavier embryos and thus converted to a larger day old chick. The performance trial performed after the incubation trial showed no significant effect of the incubation temperature on 7-day and daily mortalities, weekly bodyweight gain and feed conversion efficiency to 42 days of life. The lack of effects observed in these trials could be attributed to the small range of temperatures used in addition to the difficulties brought about the use of multi-stage incubators.

During the 21 day incubation period, the fertile egg provides nutrients such as fatty acids for energy and polyunsaturated fatty acids (PUFA) for membrane synthesis to the developing chick. The hypothesis tested in the present study is that the type of PUFA fed to the breeder hen can alter tissue lipid composition and PUFA metabolism in the progeny during growth. The objective of the present study was to test two different sources of PUFA (n-3 or n-6) on: 1) egg production, egg, and chick quality; and 2) changes in tissue PUFA composition and metabolism in progeny during growth. Fertilized eggs (n=240) were collected from Ross breeder hens (n=45) fed one of the three experimental diets containing 3.5% fish (long chain n-3), flax (18:3 n-3), or safflower oil (18:2 n-6). The egg and yolk weight was lowest for eggs from hens fed fish oil (P=0.09, P=0.02). The chick weight on day of hatch was 41.2, 45.3, and 43.3g, for fish, flax, and safflower, respectively (P=0.003). In the second experiment fertilized eggs were collected from Lohman Brown layer hens (n=75) fed a control, high n-3, or low n-3 diet. Chicks were raised up to day 14 on a control diet lacking long-chain n-6 and n-3 fatty acids. Chick tissue samples (gastrointestinal tract, liver, and blood) were collected on day 1, 7, and 14 and were subjected to fatty acid (FA) and interleukin-6 (IL-6) analysis. The long-chain n-6 to long chain n-3 ratio was lowest in the duodenum, jejunum, ileum, and liver from chicks hatched from fish oil fed hens (P<0.001) up to day 14. Interleukin-6 was lowest in liver (P=0.009) and serum on day of hatch, for fish oil chicks. The results from this study show that the diet fed to breeder hens alters progeny tissue PUFA composition and lipid metabolism during early development in avians. The long term effects of maternal diet manipulation on progeny growth and lipid metabolism need to be investigated in detail. / Graduation date: 2013

This study aimed to evaluate the effect of age and lineage of layers breeders light and semi heavy on the quality of the egg, the pinto newly eclodido and performance of incubation. 3,456 eggs were used for breeding light (Dekalb White) and semi heavy (Bovans Goldline) in three different ages (young, old and middle age). The experiment was divided into two stages, at first we evaluate the characteristics of the eggs of a sample of 360 eggs (60 per treatment). In the second stage evaluated the performance of chicks hatching and quality of a sample of 3,096 eggs (516 per treatment). It was observed that the quality of the shell fell and the weight of the egg increased with the increase of age of the birds. The layers breeders Dekalb White eggs produced heavier in the middle ages and with better quality of bark at all ages. With the increasing age of the bird has increased the percentage of yolk and albumen decreases. This decrease was more pronounced in a manner eggs line Dekalb White. Eggs of the same lineage, layers breeders young and old had a higher percentage of egg yolk. The pH of the yolk and albumen increased with the ageing of layers breeding, which in old age, the egg line Dekalb White shows higher values for these variables that line Bovans Goldline. As for the assessment of incubation found that birds' eggs old line Bovans Goldline had greater percentage of weight loss during the incubation than those from Dekalb White layers breeders of the same age. The weight of pinto increased with increasing age of the leyer breeding and Dekalb White chicks born heavier that the Bovans Goldline. However, the relationship of chicks weight / weight of the egg was higher for chicks Bovans Goldline poultry young and middle age. The progeny Dekalb White was not influenced by the age of reproductive on this parameter. The percentage of females born in respect of fertile eggs was greater in the lineage Dekalb White. Since young matrices obtained lower values for this variable, regardless of their lineage. The female chicks Dekalb White, and had weights more uniform, had a higher percentage of bag viteline than the Bovans Goldline. The age of the matrix no effect on these variables. The percentage of the heart and lungs in the weight of the female chicks were not influenced by age or the lineage of breeding. / O presente trabalho teve como objetivo avaliar o efeito da idade e da linhagem de galinhas matrizes leves e semi pesadas sobre a qualidade do ovo, do pinto recém-eclodido e do rendimento da incubação. Foram utilizados 3.456 ovos de reprodutoras leves (Dekalb White) e semi pesadas (Bovans Goldline) em três diferentes idades (jovens, velhas e de média idade). O experimento foi dividido em duas etapas; na primeira se avaliou as características dos ovos de uma amostra de 360 ovos (60 por tratamento). Na segunda etapa avaliou-se o desempenho da incubação e a qualidade dos pintinhos numa amostra de 3.096 ovos (516 por tratamento). Observou-se que a qualidade da casca diminuiu e o peso do ovo aumentou com a elevação da idade das aves. As matrizes Dekalb White produziram ovos mais pesados na idade média e com melhor qualidade de casca em todas as idades. Com o aumento da idade da ave o percentual da gema aumentou e do albúmen diminui. Essa diminuição ocorreu de maneira mais acentuada em ovos da linhagem Dekalb White. Ovos dessa mesma linhagem, de matrizes jovens e velhas apresentaram maior percentual de gema. O ph da gema e do albúmen aumentou com o envelhecimento das matrizes, sendo que na idade ve lha, o ovo da linhagem Dekalb White apresenta valores maiores para essas variáveis que os da linhagem Bovans Goldline. Quanto à avaliação da incubação verificou-se que ovos de aves velhas da linhagem Bovans Goldline apresentaram maior percentual de perda de peso durante a incubação do que aqueles provenientes de matrizes Dekalb White de mesma idade. O peso do pinto aumentou com o aumento da idade da matriz e pintinhos Dekalb White nasceram mais pesados que os Bovans Goldline. Porém, a relação peso do pinto / peso do ovo foi maior para pintos Bovans Goldline de aves jovens e de média idade. A progênie Dekalb White não foi influenciada pela idade da reprodutora quanto a esse parâmetro. O percentual de fêmeas nascidas em relação aos ovos férteis foi maior na linhagem Dekalb White. Sendo que matrizes jovens obtiveram valores menores para essa variável, independente de sua linhagem. As pintinhas Dekalb White, além de apresentaram pesos mais uniformes, obtiveram maior percentual de saco vitelino do que as Bovans Goldline. A idade da matriz não exerceu influência sobre essas variáveis. O percentual do coração e dos pulmões em relação ao peso da pintinha não foi influenciado pela idade nem pela linhagem das reprodutoras. / Mestre em Ciências Veterinárias

Thesis (M.A.)--Miami University, Dept. of Communication, 2008. / Title from first page of PDF document. Includes bibliographical references (p. 97-105).