Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-20T12:56:36Z No. of bitstreams: 0 / Rejected by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br), reason: n?o da para inserir licen?a on 2015-01-20T16:55:25Z (GMT) / Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T11:32:34Z No. of bitstreams: 2 bethania_alves_avelar1.pdf: 1374426 bytes, checksum: 09450a40db624b58e0a45e591d96b5d9 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T11:33:27Z (GMT) No. of bitstreams: 2 bethania_alves_avelar1.pdf: 1374426 bytes, checksum: 09450a40db624b58e0a45e591d96b5d9 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-02-20T11:33:27Z (GMT). No. of bitstreams: 2 bethania_alves_avelar1.pdf: 1374426 bytes, checksum: 09450a40db624b58e0a45e591d96b5d9 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2010 / O uso de plantas da fam?lia Euphorbiaceae, principalmente do g?nero Euphorbia, tem sido popularmente difundido para o tratamento de uma variedade de doen?as de natureza infecciosa, tumoral e inflamat?ria. Entre as esp?cies desse g?nero, a Euphorbia tirucalli ? uma planta de uso difundido no Brasil e em algumas regi?es, tal como a do Vale do Jequitinhonha. H? ind?cios de que o l?tex de E. tirucalli tenha atividade antitumoral e antiviral, por?m, pouco se sabe sobre os mecanismos envolvidos nessa a??o. ? prov?vel que o mecanismo de a??o para tais atividades envolva a ativa??o de leuc?citos e a produ??o de citocinas para o direcionamento de uma resposta antiviral e antitumoral efetivas. Diante disso, o presente trabalho avaliou a produ??o de citocinas TNF-?, IFN-?, IL-4 e IL-10 nas popula??es e subpopula??es leuc?cit?rias do sangue perif?rico estimulados com o l?tex bruto da referida planta. Para tal, leuc?citos do sangue perif?rico de vinte indiv?duos h?gidos foram incubados por 4 horas com o l?tex bruto de E. tirucalli dilu?do em Dimetilsulfoxido (DMSO) e constituiu o grupo l?tex (Lt). Culturas celulares incubadas com salina e DMSO, utilizando-se o mesmo per?odo de incuba??o, constitu?ram as culturas controle n?o estimuladas (CC) e controle de solvente (DMSO), respectivamente. Ap?s o per?odo de incuba??o, as c?lulas foram marcadas com anticorpos monoclonais espec?ficos para receptores de superf?cie celular CD4, CD8 e CD14, bem como para as citocinas intracitoplasm?ticas TNF-?, IFN-?, IL-4 e IL-10. A aquisi??o e an?lise dos dados foram realizadas por citometria de fluxo. Os resultados demonstraram um aumento significativo (p < 0,05) no percentual de linf?citos T e sua subpopula??o T CD4 positivos para as citocinas do tipo 1, TNF-? e IFN-?. Essas citocinas s?o respons?veis por ativar o sistema imune para respostas imunoprotetoras frente ?s infec??es virais e processos tumorais. J? os neutr?filos e linf?citos T CD8, nas culturas estimuladas com o l?tex da planta, apresentaram um perfil misto de produ??o de citocinas do tipo 1 e 2. Esses resultados evidenciaram uma resposta do tipo 1 predominante, in vitro, e uma prov?vel ativa??o de mecanismos moduladores, mediados por linf?citos T CD8 e neutr?filos. Este estudo sugere que a utiliza??o popular da planta em infec??es virais e processos tumorais pode ter algum fundamento, tendo em vista a produ??o preferencial de citocinas do tipo 1 em c?lulas estrat?gicas da resposta imune celular, fundamental no combate ?s patologias mencionadas. No entanto, os estudos in vitro aqui realizados n?o s?o suficientes para garantir que a utiliza??o da planta no contexto in vivo possa, de fato, apresentar tais propriedades. Assim, estudos adicionais, incluindo outros ensaios in vitro, bem como ensaios pr?-cl?nicos, s?o fundamentais para o avan?o das pesquisas envolvendo a utiliza??o de E. tirucalli para fins terap?uticos. / ABSTRACT The use of plants in the family Euphorbiaceae, especially of the genus Euphorbia, has been popularly circulated for the treatment of a variety of infectious, tumoral and inflammatory illnesses. Among the species of this genus, Euphorbia tirucalli is a the plant with widespread use in Brasil and in some regions, such as the Vale do Jequitinhonha. There is evidence that the latex of E. tirucalli has antiviral and antitumor activity, but little is known about the mechanisms involved in this action. It is likely that the mechanism of action for such activities involves leukocyte activation and cytokine production for directing an effective antitumor and antiviral response. Thus, the present study evaluated the production of cytokines TNF-?, IFN-?, IL-4 and IL-10 in the populations and subpopulations in peripheral blood leukocytes stimulated with crude latex of this plant. For such, peripheral blood leukocytes of twenty healthy subjects were incubated for 4 hours with crude latex of E. tirucalli diluted in dimethyl sulfoxide (DMSO) and was the latex group (Lt). Cell cultures incubated with saline and DMSO, using the same incubation period, represent the control cultures that were not stimulated (CC) and solvent control (DMSO), respectively. After the incubation period, cells were stained with monoclonal antibodies specific to cell surface receptors CD4, CD8 and CD14, as well as for TNF-? intracytoplasmic cytokines, IFN-?, IL-4 and IL-10. The acquisition and analysis were performed by flow cytometry. The results showed a significant increase (p <0.05) in the percentage of T lymphocytes and their subpopulation T CD4 positive for type 1 cytokines, TNF-? and IFN-?. These cytokines are responsible for activating the immune system to immunoprotective responses against viral infections and tumor processes. However the neutrophils and T CD8 lymphocytes, in cultures stimulated with the latex of the plant, showed a mixed profile of cytokine production of type 1 and 2.These results showed a response of the type 1 predominant, in vitro, and a probable activation of modulatory mechanisms mediated by T CD8 lymphocytes and neutrophils.This study suggests that the popular use of plant in viral infections and tumor processes may have some foundation, in order to preferential production of type 1cytokines in strategic cells of the cellular immune response, that is essential in combating the diseases mentioned. However, in vitro studies conducted here are not sufficient to ensure that the use of the plant in vivo context may, in fact, possesses such properties. / Disserta??o (Mestrado) ? Programa Multic?ntrico de P?s-gradua??o em Ci?ncias Fisiol?gicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010.

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No. of bitstreams: 0 Previous issue date: 2011-02-17 / Item withdrawn by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-01-23T15:45:24Z Item was in collections: PMPGCF - Mestrado em Ci?ncias Fisiol?gicas (Disserta??es) (ID: 103) No. of bitstreams: 4 nubia_carelli_pereira_avelar.pdf: 1444636 bytes, checksum: c9532611d0e9873ec605dd4f6820b17e (MD5) licenca_crative_commons.txt: 56 bytes, checksum: 07348f118ac3d013acae474937b75fd4 (MD5) nubia_carelli_pereira_avelar.pdf.txt: 214775 bytes, checksum: 250f168b6c41e42bef37f307694a0d04 (MD5) licenca_crative_commons.txt.txt: 53 bytes, checksum: 48f12eb36296e49e3babe2c05b2dc57e (MD5) / Item reinstated by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T13:55:52Z Item was in collections: PMPGCF - Mestrado em Ci?ncias Fisiol?gicas (Disserta??es) (ID: 103) No. of bitstreams: 4 nubia_carelli_pereira_avelar.pdf: 1444636 bytes, checksum: c9532611d0e9873ec605dd4f6820b17e (MD5) licenca_crative_commons.txt: 56 bytes, checksum: 07348f118ac3d013acae474937b75fd4 (MD5) nubia_carelli_pereira_avelar.pdf.txt: 214775 bytes, checksum: 250f168b6c41e42bef37f307694a0d04 (MD5) licenca_crative_commons.txt.txt: 53 bytes, checksum: 48f12eb36296e49e3babe2c05b2dc57e (MD5) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Quantificar o consumo de oxig?nio e a frequ?ncia card?aca durante a adi??o de vibra??o de todo o corpo aos exerc?cios de agachamento em idosos e investigar os efeitos do treinamento com exerc?cios de agachamento associados ? vibra??o de todo o corpo no desempenho funcional e no autorrelato do estado da osteoartrite de joelho em idosos foi o objetivo deste estudo. O consumo de oxig?nio e a frequ?ncia card?aca foram avaliados em repouso e durante os exerc?cios de agachamento com e sem vibra??o a 40 Hz de frequ?ncia e amplitude de 4 mm, de forma aleat?ria com intervalo m?nimo de 24 horas em 18 idosos (15 mulheres e 3 homens, com idade m?dia de 72+6 anos de idade). Para verificar os efeitos da adi??o de vibra??o de todo o corpo ao treinamento com exerc?cios de agachamento, 35 idosos com osteoartrite de joelho, com diagn?stico confirmado por exame cl?nico e radiogr?fico, foram avaliados em tr?s momentos distintos: tr?s semanas anteriores ao in?cio do programa, antes e imediatamente ap?s 12 semanas de interven??o. Os volunt?rios foram alocados aleatoriamente em tr?s grupos: um grupo de interven??o que realizou o programa de agachamento em associa??o com o est?mulo vibrat?rio, promovido pela plataforma vibrat?ria (GPV, N: 12), um grupo exerc?cio que realizou o mesmo programa de agachamento sem vibra??o (GE, N: 11) e um grupo controle que n?o realizou nenhum exerc?cio durante o per?odo do estudo (GC, N: 12). Todos os volunt?rios realizaram quatro testes de desempenho funcional, mensurados de forma direta (Escala de Equil?brio de Berg, Timed Get Up and Go, Teste de Levantar e Sentar na Cadeira e Teste de Caminhada de 6 Minutos), e avalia??o do autorrelato do estado da osteoartrite pelo Western Ont?rio McMaster Universities Osteoarthritis Index (WOMAC). A vibra??o de todo o corpo associada aos exerc?cios de agachamento promoveu um aumento adicional de cerca de 20 % no consumo de oxig?nio e de 7,5 % na frequ?ncia card?aca. Al?m disso, verificou-se que o programa proposto para o GPV aprimorou o desempenho em todos os testes funcionais e em todos os dom?nios do WOMAC. J? o GE apresentou melhora no autorrelato da dor (WOMAC) e aprimorou o desempenho apenas nos testes de Equil?brio de Berg e de Caminhada de 6 minutos. N?o houve mudan?a nos testes de desempenho funcional e nos dom?nios do WOMAC no grupo controle. Embora o est?mulo vibrat?rio tenha intensificado o consumo de oxig?nio e frequ?ncia card?aca durante os exerc?cios de agachamento, esse aumento pode ser insignificante do ponto de vista cl?nico. Al?m disso, a adi??o da vibra??o ao treino com exerc?cios de agachamento melhorou o desempenho funcional e o autorrelato do estado da doen?a em idosos com osteoartrite de joelhos. / Disserta??o (Mestrado) ? Programa Multic?ntrico de P?s-gradua??o em Ci?ncias Fisiol?gicas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2010. / ABSTRACT Quantify the oxygen consumption and heart rate when vibration is applied to whole body during squat exercises in elderly and investigate the effects of whole body vibration on the functional performance and self-reported disease status of elderly patients with knee osteoarthritis it was the objective this study. The oxygen consumption and heart rate were evaluated in 18 elderly individuals, 15 females and 3 males with a mean age of 72 ? 6 years, who were randomly submitted to 3 experimental situations. The measurements were taken during rest and during the performance of squatting exercises (8 series of 40 seconds with 40 seconds of rest between series; performing squats in 3-second cycles with 10-60 degrees of flexion) without or with vibration at a frequency of 40 Hz and amplitude of 4 mm. To verify the effects of the addition of the whole body vibration to the training with squatting exercises, 35 elderly with knee osteoarthritis, with diagnosis confirmed by clinical exams and radiographic, were evaluated at three different moments: three weeks before initiating training, immediately prior to training and immediately after 12 weeks of intervention, using four functional performance tests [the Berg Balance Scale (BBS), the Timed Get Up and Go Test (TGUG), the Chair Stand Test (CST) and the 6-Minute Walk Test (6MWT)] as well as a self-report from the patients on the status of their disease, assessed using the Western Ontario McMaster Universities Osteoarthritis Index (WOMAC). The participants were randomized into three groups: in the first group, patients performed squatting exercises on a vibrating platform over a 12-week period (vibration group; n=12); in the second group, patients performed squatting exercises without vibration over the same 12-week time period (exercise group; n=11); while the third group of patients were given no exercise training during the study (control group; n=12). Associating whole body vibration with squatting exercises resulted in an additional increase of 20% in oxygen consumption and 7.5% in heart rate in elderly. In addition, whole body vibration training associated with squatting exercises improved performance in all the functional tests and in all the domains of the WOMAC scale. In the exercise group, performance improved BBS and 6MWT and in the pain domain of the WOMAC scale. No change from baseline occurred in any of the tests performed on patients in the control group. Although the vibratory stimulus has increased oxygen consumption and heart rate during squatting exercises, this elicited increase may be insufficient to improve cardiovascular fitness. Moreover, vibration training associated with squatting exercises improved functional performance measured both directly and indirectly in elderly patients with knee osteoarthritis.