Thesis (Ph.D. (Biochemistry)) --University of Limpopo, 2009 / Refer to document / National Research Foundation (NRF) and University of Limpopo

Made available in DSpace on 2014-06-11T19:28:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-06Bitstream added on 2014-06-13T19:16:34Z : No. of bitstreams: 1 ferreiraneto_me_me_jabo.pdf: 1445654 bytes, checksum: b179a42d4eeb46cdbbec60b82a6761b5 (MD5) / Monsanto / A adoção da tecnologia Roundup-Ready® de controle de plantas daninhas na cultura da soja foi bastante rápida e extensa expondo-a a uma grande diversidade de situações de composição especifica de comunidades infestantes, características edáficas e climáticas e de práticas agrícolas. Em alguns anos, passaram a serem identificadas plantas daninhas com maiores dificuldades de controle pelo glyphosate e que passaram a ser selecionadas e suas importâncias aumentadas nos agroecossistemas. Com o objetivo de estudar o controle de plantas daninhas em soja geneticamente modificada para tolerância ao glyphosate dois ensaios foram conduzidos um a campo e outro em condições de casa de vegetação. O ensaio de campo foi conduzido no Pontal do Paranapanema e foram avaliados os controles de plantas daninhas e seletividade à cultura da soja do glyphosate aplicado isolado e em combinação com diclosulam, cloransulam-methyl, flumioxazin e s-metolachlor. Foram dois grupos de tratamentos: um com aplicação única e outro com aplicação seqüencial de glyphosate. No campo, o experimento obedeceu ao delineamento em blocos ao acaso. Os resultados mostraram que aplicações seqüenciais proporcionaram melhor controle de Commelina benghalensis, mas não incrementaram o de Ipomoea triloba. Os melhores níveis de produtividade foram obtidos nas parcelas tratadas com a combinação de flumioxazin ou s-metolachlor com glyphosate, com ou sem aplicação seqüencial. Um segundo grupo de ensaios foi conduzido em vasos com o objetivo de avaliar a atividade biológica de diferentes doses e formulações do glyphosate em Macroptilium lathyroides, Euphorbia heterophylla e Senna obtusifolia em estádio de desenvolvimento em que regularmente há dificuldade de controle nas condições de campo. Todos os ensaios obedeceram ao delineamento... / The adoption of the Roundup-Ready® technology for weed control in soybeans was very quick and extensive. With the geographic expansion, the Roundup-Ready® soybeans met a big diversity of situations of weed composition, spil and climate features, and agronomic practices. After some years, certain weeds were identified as more tolerant to glyphosate and were selected in Roundup-Ready® soybean growing areas. So, two assays were carried out aiming to evaluate the weed control in Roundup-Ready® soybean. The first experiment was conducted under field conditions and the use of glyphosate alone or mixed with diclosulam, cloransulammethyl, flumioxazin, and s-metolachlor were evaluated under two situations: single spraying or followed of a complementary spraying of glyphosate, 14 days after the first one. The complementary application of glyphosate increased the Commelina benghalensis control, but did not change Ipomoea triloba control. The better productivity levels were achieved using the treatments with flumioxazin or s-metolachlor + glyphosate, with or without the complementary applicatrin. The second group of assays was carried under pot conditions out aiming to evaluate the biological activity of different doses and formulations of glyphosate in the control of Macroptilium lathyroides, Euphorbia heterophylla and Senna obtusifolia at growth stage in which some control difficulties were reported, under field conditions. A greenhouse essay was carried out under for each weed in the completely randomized experimental design with four replications. The treatments were arranged in a 3 x 4 factorial design considering three commercial formulations (Roundup Original®, Roundup-Ready® e Roundup Ultra®) and four doses (2,32; 1,68; 1,15 e 0,57 kg.ha-1) of glyphosate. Additionally, a control plot without chemical control was considered. The results showed that the three weeds had differential... (Complete abstract click electronic access below)

Orientador: Robinson Antonio Pitelli / Banca: Ricardo Victória Filho / Banca: Silvano Bianco / Resumo: A adoção da tecnologia Roundup-Ready® de controle de plantas daninhas na cultura da soja foi bastante rápida e extensa expondo-a a uma grande diversidade de situações de composição especifica de comunidades infestantes, características edáficas e climáticas e de práticas agrícolas. Em alguns anos, passaram a serem identificadas plantas daninhas com maiores dificuldades de controle pelo glyphosate e que passaram a ser selecionadas e suas importâncias aumentadas nos agroecossistemas. Com o objetivo de estudar o controle de plantas daninhas em soja geneticamente modificada para tolerância ao glyphosate dois ensaios foram conduzidos um a campo e outro em condições de casa de vegetação. O ensaio de campo foi conduzido no Pontal do Paranapanema e foram avaliados os controles de plantas daninhas e seletividade à cultura da soja do glyphosate aplicado isolado e em combinação com diclosulam, cloransulam-methyl, flumioxazin e s-metolachlor. Foram dois grupos de tratamentos: um com aplicação única e outro com aplicação seqüencial de glyphosate. No campo, o experimento obedeceu ao delineamento em blocos ao acaso. Os resultados mostraram que aplicações seqüenciais proporcionaram melhor controle de Commelina benghalensis, mas não incrementaram o de Ipomoea triloba. Os melhores níveis de produtividade foram obtidos nas parcelas tratadas com a combinação de flumioxazin ou s-metolachlor com glyphosate, com ou sem aplicação seqüencial. Um segundo grupo de ensaios foi conduzido em vasos com o objetivo de avaliar a atividade biológica de diferentes doses e formulações do glyphosate em Macroptilium lathyroides, Euphorbia heterophylla e Senna obtusifolia em estádio de desenvolvimento em que regularmente há dificuldade de controle nas condições de campo. Todos os ensaios obedeceram ao delineamento... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The adoption of the Roundup-Ready® technology for weed control in soybeans was very quick and extensive. With the geographic expansion, the Roundup-Ready® soybeans met a big diversity of situations of weed composition, spil and climate features, and agronomic practices. After some years, certain weeds were identified as more tolerant to glyphosate and were selected in Roundup-Ready® soybean growing areas. So, two assays were carried out aiming to evaluate the weed control in Roundup-Ready® soybean. The first experiment was conducted under field conditions and the use of glyphosate alone or mixed with diclosulam, cloransulammethyl, flumioxazin, and s-metolachlor were evaluated under two situations: single spraying or followed of a complementary spraying of glyphosate, 14 days after the first one. The complementary application of glyphosate increased the Commelina benghalensis control, but did not change Ipomoea triloba control. The better productivity levels were achieved using the treatments with flumioxazin or s-metolachlor + glyphosate, with or without the complementary applicatrin. The second group of assays was carried under pot conditions out aiming to evaluate the biological activity of different doses and formulations of glyphosate in the control of Macroptilium lathyroides, Euphorbia heterophylla and Senna obtusifolia at growth stage in which some control difficulties were reported, under field conditions. A greenhouse essay was carried out under for each weed in the completely randomized experimental design with four replications. The treatments were arranged in a 3 x 4 factorial design considering three commercial formulations (Roundup Original®, Roundup-Ready® e Roundup Ultra®) and four doses (2,32; 1,68; 1,15 e 0,57 kg.ha-1) of glyphosate. Additionally, a control plot without chemical control was considered. The results showed that the three weeds had differential... (Complete abstract click electronic access below) / Mestre

Thesis (M.Sc. (Biochemistry )) --University of Limpopo, 2007 / Refer to document / The Cannon Collins Trust Fund and the National Research Foundation

Thesis (Ph.D. (Biochemistry)) --University of Limpopo, 2009 / Commelina benghalensis Linn is used in traditional medicine in several Asian and African countries for the treatment of various ailments such as stomach irritations, burns, sore throat and feet, diarrhoea and as an anti-inflammatory agent. Recently, our laboratory showed that the crude methanolic extract of Commelina benghalensis L (CMECB) exhibits growth inhibitory and proapoptotic effects in Jurkat T and Wil-2 NS cancer cell lines. In this study, the precise molecular mechanism(s) associated with CMECB-induced growth inhibitory and apoptosis inducing effects in Jurkat T and Wil-2 NS cell lines were investigated. This was achieved by investigating the effects of the extract on the cell division cycle distribution profile as well as its effects on various cell division cycle and apoptosis regulatory genes. Ground stems of C. benghalensis L were extracted with absolute methanol to obtain a crude extract. To assess the effect of CMECB on cancer cell growth, experimental cell cultures were exposed to various concentrations (0 to 600 μg/ml) of CMECB for up to 72 hours. The results demonstrated a significant reduction in cell viability and inhibition of proliferation of experimental cell cultures as determined by the trypan blue dye exclusion assay and the Coulter counter method, respectively. Analysis of nuclear morphological changes in cells stained with Hoechst 33258 confirmed apoptosis as the mode of cell death that is associated with the growth inhibitory effects of CMECB in both the Jurkat T and Wil-2 NS cell lines. This assertion was based on the observed presence of nuclear morphological changes such as chromatin condensation and fragmentation and apoptotic bodies in cells exposed to CMECB. In order to get an insight on the pro-apoptotic mechanisms of CMECB, Western blot xxi and quantitative real-time PCR (qrt-PCR) were used to investigate the expression profiles of various apoptosis and cell division cycle regulatory genes. Qrt-PCR results showed a lack of a clear up- and/or down-regulatory effects of CMECB on the mRNA expression levels of bax and bcl-2 in both Jurkat T and Wil-2 NS cells. Western blot analyses demonstrated that CMECB induced apoptosis by facilitating Bax protein translocation from the cytosol to the mitochondria in both Jurkat T and Wil-2 NS cells. In addition, CMECB down-regulated Bcl-2 protein expression which, as a result, led to the shift in the Bax/Bcl-2 protein ratio at certain time points and concentration in both Jurkat T and Wil-2 NS cells. The modulation of the Bcl-2 family members led to mitochondrial cytochrome c release into the cytosol and activation of caspases-9 and -3; this was also confirmed by caspase activity assays and eventual degradation of PARP. Furthermore, CMECB induced Jurkat T and Wil-2 NS cell division cycle arrest at the G2/M phase as determined by flow cytometric analysis. Western blot analyses of G2/M phase regulatory proteins demonstrated that the CMECB-induced cell division cycle arrest was associated with the downregulation of cyclin B1 and Cdc2 protein expression levels. Western blot analyses results further revealed that the arrest of Wil-2 NS cells at the G2/M phase was independent of p21 protein activity. However, Jurkat T cell division cycle arrest was found to be mediated, in part, by p21. Quantitative real-time PCR results did not show a clear trend in terms of the down- or up-regulatory effects of the extracts on the G2/M phase regulatory genes. The CMECBinduced apoptosis and G2/M arrest was found to occur in a p53-independent xxii manner due to the lack and down-regulation of p53 protein levels in both Jurkat T and Wil-2 NS cells, respectively. In conclusion, CMECB induces its anticancer activity by inducing G2/M phase arrest and mitochondrial-mediated apoptosis independent of p53 protein activity. Although the study did not perform in vivo experiments to ascertain the efficacy of extracts of CMECB against specific tumour types in animal models, the present findings somehow validate the traditional use of C. benghalensis L as an anticancer agent. A more definitive study needs to be done to ascertain this assertion. / National Research Foundation and the University of Limpopo research office

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T13:30:34Z No. of bitstreams: 5 37.pdf: 3780972 bytes, checksum: 25735fd7c2f94a2a52a7a996adefcb6b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-27T18:41:07Z (GMT) No. of bitstreams: 5 37.pdf: 3780972 bytes, checksum: 25735fd7c2f94a2a52a7a996adefcb6b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) / Made available in DSpace on 2015-02-27T18:41:07Z (GMT). No. of bitstreams: 5 37.pdf: 3780972 bytes, checksum: 25735fd7c2f94a2a52a7a996adefcb6b (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2109 bytes, checksum: aa477231e840f304454a16eb85a9235f (MD5) Previous issue date: 2011 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Avaliaram-se neste trabalho os efeitos da competi??o entre cultivares de milho, feij?o e mandioca e seis esp?cies de plantas daninhas no ac?mulo e na aloca??o de mat?ria seca e nutrientes pelas plantas; o potencial de algumas dessas esp?cies em reciclar nutrientes e a efici?ncia nutricional do feijoeiro. Para isso foi realizado um experimento para cada cultura com tr?s gen?tipos de milho, tr?s de feij?o e dois de mandioca, cultivados em competi??o com seis esp?cies de plantas daninhas, al?m dos tratamentos adicionais, correspondentes ?s cultivares de milho, feij?o e mandioca e ?s esp?cies daninhas ausentes de competi??o. O per?odo de conviv?ncia entre as cultivares de milho, feij?o e mandioca e as plantas daninhas foi de 60, 45 e 75 dias ap?s emerg?ncia das culturas, respectivamente. Para a cultura do milho e do feij?o foi avaliada a aloca??o de mat?ria seca e de nutrientes, inclusive entre os diferentes ?rg?os. Ainda para o feij?o foram estimados os coeficientes para efici?ncia de absor??o, transporte e utiliza??o dos nutrientes. Para a cultura da mandioca, foram determinadas, al?m da aloca??o de mat?ria seca, caracter?sticas morfofisiol?gicas ligadas ? ?rea foliar. A folha e o caule do milho foram os principais ?rg?os afetados negativamente pela competi??o, enquanto as ra?zes das esp?cies competidoras foram os ?rg?os mais prejudicados. Em competi??o com o feijoeiro, as folhas e o caule das esp?cies daninhas foram os ?rg?os mais prejudicados. Com mandioca, os componentes vegetativos das plantas daninhas sofreram reduzidas modifica??es, sem preju?zos quanto ? produ??o de mat?ria seca. As ra?zes do feijoeiro e de mandioca foram os principais ?rg?os afetados negativamente pela competi??o. Os gen?tipos AL 25 (milho) e IPR Colibri (feij?o) foram os que menos toleraram a competi??o. De forma contr?ria, as cultivares P?rola (feij?o) e Periquita (mandioca) demonstraram possuir as maiores habilidades competitivas. O milho mostrou-se sens?vel ? competi??o com B. brizantha e C. benghalensis e competitivo com B. plantaginea. Esta esp?cie, entretanto, mostrou-se altamente competitiva com feij?o e mandioca. Al?m disso, A. spinosus, em competi??o com o feijoeiro, demonstrou possuir a maior habilidade competitiva. A capacidade competitiva entre esp?cies vegetais, quando se trata do ac?mulo de mat?ria seca e relativo de nutrientes, n?o representou vantagem para as esp?cies de plantas daninhas, quando elas foram mantidas em conviv?ncia com o milho. O feijoeiro apresentou baixa capacidade de competi??o por nutrientes, particularmente N e P. A ?rea foliar espec?fica e a raz?o de ?rea foliar da mandioca n?o foram afetadas pela competi??o. B. brizantha e C. echinatus, livre da conviv?ncia com o milho, apresentaram elevado potencial em reciclar nutrientes. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Produ??o Vegetal, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT There were evaluated in this study the effects of competition between maize, beans and cassava, and six weed species in the accumulation and allocation of dry matter and nutrients by plants, the potential of some of these species in recycling nutrients and nutritional efficiency of bean. For this end an experiment was performed to each culture with three genotypes of maize, beans and two of three cassava grown in competition with six weeds species, apart from additional treatment corresponding to the maize, beans and cassava cultivars and weed species absent from the competition. The period of coexistence among maize, beans and cassava cultivars and the weeds was 60, 45 and 75 days after crop emergence, respectively. For the corn and beans there were evaluated allocation of dry matter and nutrients, including between different organs. Even for the beans there were estimated coefficients for uptake, transport and utilization of nutrients. For cassava plants there were determined, besides the allocation of dry matter, morphological and physiological characteristics related to leaf area. The leaf and stalk of corn were the main adversely affected organs by competition, while the roots of competing species were the most affected organs. In competition with the beans, leaves and stems of weeds were the most affected organs. With cassava, the weeds vegetative components suffered reduced modifications without losses in the production of dry matter. The roots of bean and cassava were the main adversely affected organs by competition. Genotypes AL 25 (maize) and IPR Colibri (beans) were the less tolerant in the competition. Conversely, the P?rola (beans) and Periquita (cassava) have showed the greatest competitive abilities. The corn was sensitive to competition with B. brizantha and C. benghalensis and competitive with B. plantaginea. This species, however, proved to be highly competitive with beans and cassava. Furthermore, A. spinosus, in competition with the bean, has demonstrated the greatest competitive ability. The competitive ability of plant species when it comes to the accumulation of dry matter and related nutrients did not represent the advantage to the weeds, where they were kept in close contact with the corn. The bean had low capacity to compete for nutrients, particularly N and P. The specific leaf area and leaf area ratio of cassava were not affected by competition. B. brizantha and C. echinatus, free of coexistence with the corn, had high potential to recycle nutrients.

Made available in DSpace on 2016-02-26T14:53:09Z (GMT). No. of bitstreams: 1 EvanirSilvaMartinsCarvalho.pdf: 3597617 bytes, checksum: 21b7c5a6853a920243c751376e6b66fa (MD5) Previous issue date: 2007-03-29 / This work was done in order to verify population fluctuation, alternative hosts, and the natural occurrence of parasitoids in eggs and adults of the green belly stink bug, Dichelops melacanthus (Dallas, 1851), in No-Tillage System in the South Region of Mato Grosso de Sul State. Weekly samples were taken - from September 2005 to December 2006 on soybean, second season corn crop, on wheat and on volunteer corn plants, in the middle of straws and on weeds. All the field observations were done in areas of Ponta Porã, Itaporã and Dourados, in Mato Grosso do Sul State, Brazil, where were also collected forty adults and their egg masses to observe parasitism in the lab. The insect was detected at the three experiments throughout the year, but peaks above 1.0 stink bug/m2 were observed more frequently in Ponta Porã, from February to December 2006. In Ponta Porã was also registered, soon after the soybean harvesting - in the middle of the remaining straw - in the presence of volunteer corn plants, the highest of all the peaks 4.1 (±1,45) insects/m2 in April 2006. In Itaporã, the population density reached the magnitude of 1.0 (±0,38) D. melacanthus/m2, in November 2005, also on volunteer corn plants. Approached of the same level in December 2006, at the initial vegetative growth stage of the soybean in the presence of volunteer corn plants and reached the uppermost level 1.5 (±0,53) individual/m2 in September and 1.5 individual/m2 (±0,26) in November 2006, both on volunteer corn plants. As for Dourados, more than 1.0 (±0,31) stink bug/m2 was only registered in September 2006, on volunteer corn plants;approached of the same level in October and November of the same year, on volunteer corn plants, and on soybean at early vegetative growth stage associated with volunteer corn plants. By and large, the highest population peaks occurred near the soybean harvesting; in the middle of the remaining straw - soon after the soybean harvesting mostly in the presence of volunteer corn plants; at the initial growth stages of the second season corn crop and of the volunteer corn plants. The same occurred during the entire permanence of the wheat crop in the field, mostly at its initial development stages. The contrary happened during the soybean vegetative and early reproductive growth stages, except when they were in the presence of volunteer corn plants. It was also observed that when the second season corn crop and the volunteer corn plants have developed, the population became lower. Parasitism exerted in adults by three different species of Tachinidae occurred in almost all months of the year. Individuals collected in Itaporã, in March 2006, did not exhibit parasitism. However, 53% of the insects collected in November were parasitized. The highest level of parasitism exerted in the stink bug D. melacanthus eggs - by the Scelionidae Telenomus podisi Ashmead (1893) were 38,4 31,5 and 45,0%, in Ponta Porã; 42,3%, in Itaporã and 46,1%, in Dourados. Among the weeds, the four favorite alternative hosts are Cenchrus echinatus L., Chloris gayana Kunth, Panicum maximum Jacq. and Commelina benghalensis L / Este trabalho teve os objetivos de verificar a flutuação populacional, os hospedeiros alternativos e a ocorrência natural de parasitóides em ovos e adultos do percevejo-barrigaverde, Dichelops melacanthus (Dallas, 1851), em solos explorados no Sistema Plantio Direto no Sul de Mato Grosso do Sul. As amostragens foram realizadas, semanalmente, de setembro/05 a dezembro/06, nas culturas da soja, milho-safrinha e trigo, em plantas voluntárias de milho, no meio da palhada e em ervas daninhas. As observações de campo foram feitas em experimentos localizados em áreas de Ponta Porã, Itaporã e Dourados, onde também foram coletados, quarenta adultos de D. melacanthus e suas posturas, para observação de parasitismo em laboratório. O inseto foi detectado o ano todo nos três experimentos, mas, picos acima de 1,0 percevejo/m2 ocorreram com maior freqüência, de fevereiro a novembro/06. Em Ponta Porã também foi observado - logo após a colheita da soja, na palha associada a plantas voluntárias de milho - o maior de todos os picos 4,1 (±1,45) percevejo/m2, em abril/06. Em Itaporã, a população alcançou a magnitude de 1,0 (±0,38) percevejo/m2, em novembro/05, em plantas voluntárias de milho; aproximou-se do mesmo nível em dezembro/06, em soja de fase inicial do estádio vegetativo, na presença de plantas voluntárias de milho; e culminou em 1,5 (±0,53) percevejo/m2, em setembro e 1,5 (±0,26) percevejo/m2 em outubro/06, ambos na presença de plantas voluntárias de milho. Quanto a Dourados, registrou-se densidade superior a 1,0 (±0,31) percevejo/m2, em plantas voluntárias de milho, apenas em setembro/06; aproximou-se do mesmo nível em outubro/novembro do mesmo ano, em plantas voluntárias de milho e em soja em estádio reprodutivo inicial associado a plantas voluntárias de milho, respectivamente. De modo geral, os maiores auges populacionais ocorreram nas proximidades da colheita da soja; na palha da soja, logo após a colheita, principalmente na presença de plantas voluntárias de milho; nos estádios iniciais do milho safrinha e das plantas voluntárias de milho. O mesmo ocorreu durante todo o tempo de permanência do trigo no campo, principalmente no estádio inicial do desenvolvimento deste cereal. Deu-se o contrário nos estádios vegetativos da soja e durante as fases iniciais do seu estádio reprodutivo, exceto quando as plantas estiveram na presença de plantas voluntárias de milho. Observou-se também que à medida que a cultura do milho safrinha e as plantas voluntárias de milho cresceram, a população do inseto diminuiu. O parasitismo exercido em adultos por três diferentes espécies de Tachinidae se fez presente em quase todos os meses do ano. Indivíduos coletados em Itaporã, em março/06, não foram parasitados, mas 53% dos observados no mês outubro, sim. Os maiores índices de parasitismo exercido em ovos de D. melacanthus pelo Scelionidae Telenomus podisi Ashmead, 1893 foram: 38,4; 31,5 e 45,0%, em Ponta Porã; de 42,3% em Itaporã e de 46,1%, em Dourados. Entre as plantas não cultivadas, os quatro hospedeiros alternativos preferidos são: Cenchrus echinatus L., Chloris gayana Kunth, Panicum maximum Jacq. e Commelina benghalensis L