Emotion Regulation describes the ability to influence the experience and expression of affect. Adaptive emotion regulation contributes to healthy development, social competence and academic success (Kochanska, Murray & Harlan, 2000). This study investigated the behavioural strategies for emotion regulation, emotion expression, regulatory styles and classroom behaviour in middle childhood. One hundred and twenty-eight children were recruited from five UK public and private primary schools. From within their school setting, participant sensitivity to emotion-eliciting events was recorded using ambulatory skin conductance technology whilst age-group paired children performed two LEGO construction tasks. Observed behaviours were video-recorded and coded to establish frequencies of distinct regulatory behaviours. These were compared to self-reports of emotion regulation strategies and teacher-reports of classroom behaviour. Iterative partitioning cluster analysis methods were used to identify four regulatory profiles: 1) the ‘Adaptive’ cluster: employed high levels of positive problem solving and reappraisal strategies and frequently expressed both positive and negative emotions; 2) the ‘Maladaptive’ cluster: used more negative regulation (avoidant or obstructive strategies), expressed more negative emotion and had more social and behavioural problems in class; 3) the ‘Reactive’ cluster showed high levels of electrodermal activity, expressed little emotion and were reported as inattentive/hyperactive in class; and 4) the ‘Distracted’ cluster demonstrated high levels of behavioural and cognitive distraction. These results indicate four meaningful profiles that could support the identification of vulnerable individuals for positive school-based intervention and support.

Estômatos são poros responsáveis pela troca gasosa entre a folha e o meio externo. A partir da década de 80, experimentos revelaram um complexo padrão espaço temporal na abertura e fechamento dos estômatos. As experiências apontam para uma possível coordenação entre estômatos em algumas áreas da folha chamada de patches. Esse fenômeno é conhecido na literatura como patchy stomatal conductance. Frequentemente a coordenação dinâmica dos estômatos está associada à oscilações temporais na condutãncia estomática (média especial da abertura dos estômatos). Em 1997 Haefner, Buckley e Mott (HBM) publicaram uma análise numérica de um modelo dinâmico para explorar o comportamento complexo dos estômatos. O modelo é baseado em algumas características conhecidas dos estômatos e assume transporte hídrico em uma rede definida por uma geometria simples e bastante restritiva. De acordo com os autores, o modelo reproduz qualitativamente os dados experimentais. Recentemente, Ferraz e Prado mostraram que esse modelo não é capaz de reproduzir os resultados experimentais. Usando ingredientes do modelo sugerido por HBM, Ferraz e Prado sugeriram uma geometria realística de distribuição reservatórios hídricos. Embora essa configuração reproduza os patches, eles permanecem estáticos e nenhuma oscilação é observada. Sem explorar detalhes significativos, Ferraz e Prado afirmaram que a histerese na abertura estomatal poderia explicar vários aspectos dos resultados experimentais. No presente estudo comprovamos, através de uma abordagem computacional baseada em transdutores histeréticos, que a hipótese de histerese na abertura dos estômatos de fato reproduz qualitativamente os dados experimentais. Em nossa abordagem a histerese na abertura dos estômatos é emulada através de operadores chamados de histerons. A robustez da hipótese é testada usando diferentes tipos de histerons. Analisamos a correlação entre os estômatos na rede que simula a superfície da folha. Observamos que a correlação entre estômatos depende da geometria da veia. Uma análise detalhada dos parâmetros envolvidos revela uma dependência entre o período de oscilação na condutância estomática e o déficit de vapor d\'água entre a folha e o meio ambiente. Esta característica subjacente ao modelo pode inspirar novas experiências para testar a hipótese da histerese na abertura dos estômatos. / Stomata are pores on the surface of leaves responsible for controlling the exchange of gas between the plant and the environment. Experiments revealed a complex spatial-temporal pattern in the opening and closing mechanism of stomata. The main feature of the phenomenon is that stomata appear to be synchronized into clusters, known as patches. The dynamical coordination of stomata often involves oscillations in stomatal conductance. In 1997 Haefner, Buckley, and Mott (HBM) published a numerical analysis of a dynamic model to explore the complex behavior of stomata. The model is based on some known features of the stomata, and assumes that water diffuses within the leaves according to a simple geometric arrangement. According to the authors, the model reproduces qualitatively the experimental data. Recently, Ferraz and Prado showed that the computational approach of HBM is not able to reproduce the experimental results. Inspired by this model, Ferraz and Prado introduced a new geometric features that leads to static patches of stomata; however no oscillation was observed and the patches remained static. The authors suggested that hysteresis in stomatal aperture could explain several experimental aspects. We now report a further investigation of the changes suggested by Ferraz and Prado in the original model of HBM. The theoretical approach confirmed that hysteresis in the aperture mechanism of pores reproduces a variety of behaviors of stomatal conductance described in experiments. We explore the hysteresis feature through the formalism of hysteretic transducer. The robustness of the hysteretic assumption is tested by different kinds of hysteresis operators. We analyzed the correlation among stomata in the lattice. We observed that the correlation depends on the geometry of the veins. Finally, the analysis of the model reveals a dependence between the period of oscillation in the stomatal conductance time series and water vapor pressure deficits Δω - an external parameter. Further experiments might explore this underlying feature of the model.

Filmes de Langmuir de fosfolipídios e ácidos graxos foram caracterizados através de medidas de pressão e potencial de superfície e condutância lateral. O objetivo principal foi o de se fazer um estudo crítico de artigos apresentados na literatura, os quais trazem interpretações errôneas para resultados do potencial de superfície, sugerem a heterogeneidade dos filmes, ou a inexistência do aumento da condutância devido a compressão de um filme de Langmuir. Comprovamos, inicialmente, a homogeneidade macroscópica dos filmes de ácidos graxos e fosfolipídios, medindo-se o potencial com a prova de Kelvin em três posições diferentes. Nenhuma alteração significativa foi registrada. O potencial e a pressão de superfície são nulos para grandes áreas por molécula, sendo que o potencial só se torna não nulo após um aumento abrupto, que ocorre a uma certa área critica. Estes resultados, juntamente com a ausência de histerese nas medidas de pressão e potencial de superfície nos ciclos de compressão-expansão, denotam a inexistência de agregados de dimensões macroscópicas. Eles contradizem, entretanto, a asserção de alguns pesquisadores na ultima conferencia de filmes Langmuir-Blodgett, de que a presença de domínios microscópicos nos filmes de Langmuir deveria afetar os resultados do potencial de superfície. Podemos concluir, portanto, que quaisquer irreprodutibilidades do potencial de superfície para grandes áreas por molécula devem ser atribuídas a impurezas do sistema de medidas, principalmente da subfase. As medidas de condutância foram realizadas diretamente, medindo-se a corrente entre dois eletrodos de platina parcialmente imersos na subfase. Para grandes áreas por molécula, a condutância medida é devido a condutividade de volume da água ultrapura, mas quando o filme e comprimido para áreas menores do que uma certa área critica (a mesma na qual e observado o aumento abrupto do potencial), a condutância sobe ate a pressão de superfície começar a subir, atingindo valores da ordem de 10-8s acima da condutância da subfase. Foram realizadas medidas para um fosfolipídio e um fosfato. Com isso, corroboramos os resultados de condutância, da mesma ordem de grandeza, obtidos em Bangor, no Reino Unido, ao mesmo tempo que são refutadas as observações feitas por grupos de pesquisa dos Estados Unidos e da Rússia. As possíveis causas para a não observação da condutância por esses últimos grupos podem ser o efeito do menisco - queda da área imersa dos eletrodos quando do aumento da pressão de superfície - que mascara o aumento da condutância, ou artefatos experimentais, tais como impurezas na subfase / Langmuir films from phospholipids and fatty acids were characterized using surface pressure, surface potential and lateral conductance measurements. The main aim of this project was to undertake a critical study of articles published in the literature which present erroneous interpretations for surface potential results, suggest non-homogeneity of Langmuir films, or rule out the possible increase in conductance upon the compression of a monolayer. First, we demonstrate that phospholipids and fatty acid mono layers are homogeneous at the macroscopic level, by measuring their surface potential with the Kelvin probe on three different positions. No significant difference was observed. The surface potential and surface pressure is zero for large areas per molecule, and the surface potential only becomes non-zero when an abrupt increase takes place at a given critical area. Together with the absence of hysteresis in the surface pressure and surface potential measurements in the compression-expansion cycles, these results indicate the absence of aggregates (or islands) of macroscopic dimensions. They contradict, however, the opinion of some authors at the last Conference on Langmuir-Blodgett Films, who took the view that the presence of micro domains on Langmuir monolayers should affect the surface potential measurements. We can conclude, therefore, that any non-reproducibility of surface potentials at large areas per molecule must be attributed to impurities in the system, especially in the sub-phase. The lateral conductance measurements were performed by measuring the current between two platinum electrodes, which were partially immersed into the sub-phase. For large areas per molecule, the conductance is due to the bulk conductivity of ultra pure water, but when the monolayer was compressed below a given critical area (the same in which a sharp increase in surface potential was observed), the conductance increases until the surface pressure starts to rise, reaching values of the order of 10-8s above the water conductance. Measurements were taken for one phospholipids and one phosphate. This corroborates the results published by the Bangor Group (UK), at the same time that observations made by an American Group and a Russian Group is contradicted. The possible causes for these groups being unable to observe the increase in conductance can be the meniscus effect caused by the decrease of the electrodes immersed area when the pressure rises - which overrun the conductance increase, and/or experimental artifacts, possibly caused by impurities in the sub-phase

Hyperkalemic periodic paralysis (HEPP) is an inherited, autosomal disorder characterized by myotonia and periodic paralysis in skeletal muscle. The hallmark of the disease is a severe sensitivity to the K+-induced force depression, the cause of the paralysis. Previous studies have provided evidence that the sensitivity to the K+-induced force depression can be alleviated when the Cl- conductance (GCl) is lowered. However, those studies were carried out at non-physiological temperatures (25°-30°C) and few stimulation frequencies. The overarching goal of this study was to examine whether manipulating GCl pharmacologically was a viable target for treating HEPP. This work sought to document the interactive effect of K+ and Cl- on force development in mouse skeletal muscle at 37°C, over a wide range of stimulation frequencies. Secondly, experiments were undertaken to determine if a reduction in GCl could protect against the severe K+ sensitivity in HEPP. The results show that in wildtype muscle, a reduction in GCl improved force generation at high [K+]e at stimulation frequencies that naturally occur in vivo for mouse EDL and soleus. While the effect in wildtype muscles was proof of principle that a reduction in GCl may be a potential approach to treat HEPP patients, the effects of reduced GCl at high [K+]e was quite variable in HEPP muscles. In a few cases, lowering GCl did improve force generation at high [K+]e. However, in most cases the decrease in GCl exacerbated the force depression at high [K+]e, suggesting that more studies will be necessary to understand the variability in the Cl- effect to conclude whether a decrease in GCl is a viable approach to treat HEPP patients.

The immediate goal of this thesis was the analysis of a three-component system whose major component was water. This analysis was to be the result of a procedure which could be readily extended to more complex systems.

Microchannels and micro pin fins have been employed for almost four decades in the cooling of microelectronic devices and research is still being done in this field to improve the thermal performance of these micro heat sinks. In this research, the constructal design and computational fluid dynamics code was used with a goal-driven optimisation tool to numerically investigate the thermal performance of a novel design of combining microchannels and micro pin fins for microelectronic cooling applications. Existing designs of microchannels were first optimised and thereafter, three to seven rows of micro pin fins were inserted into the microchannels to investigate whether there was further improvement in thermal performance. The microchannels and micro pin fins were both embedded in a highly conductive solid substrate. three-dimensional geometric structure of the combined micro heat sink was optimised to achieve the objective of maximised thermal conductance, which is also minimised thermal resistance under various design conditions. The micro heat sinks investigated in the study were the single microchannel, two-layered microchannels with parallel and counter flow configurations, three-layered microchannels with parallel and counter flow configurations, the single microchannel with circular-, square- and hexagonal-shaped micro pin-fin inserts and the two-layered microchannels with circular-shaped micro pin-fin inserts. A numerical computational fluid dynamics (CFD) package with a goal-driven optimisation tool, which employs the finite-volume method, was used to analyse the fluid flow and heat transfer in the micro heat sinks investigated in this work. The thermal performances of all the micro heat sinks were compared for different application scenarios. Furthermore, the temperature variation on the heated base of the solid substrate was studied for the different micro heat sinks to investigate which of the heat sink designs minimised the temperature rise on the heated base best. This is very important in microelectronic cooling applications because temperature rise affects the reliability of the device. The heat sink design that best maximised thermal conductance and minimised temperature rise on the heated base was chosen as the best for microelectronic cooling. For all the cases considered, fixed volume constraints and manufacturing constraints were applied to ensure real-life applicability. It was concluded that optimal heat sink design for different application scenarios could be obtained speedily when a CFD package which had an optimisation tool was used. / Thesis (PhD)--University of Pretoria, 2016. / Mechanical and Aeronautical Engineering / PhD / Unrestricted

The continual advance in manufacturing processes has resulted in significantly more compact, high performance, devices. Consequently, heat extraction has become the limiting factor, and of primary concern. Therefore, a substantial amount of research has been done regarding high efficiency micro heat exchangers, employing novel working fluids. This dissertation numerically investigated the thermal behaviour of microchannel elements cooled by Newtonian and non-Newtonian fluids, with the objective of maximising thermal conductance subject to constraints. This was done, firstly, for a two-dimensional simple microchannel, and secondly, for a three-dimensional complex microchannel. A numerical model was used to solve the governing equations relating to the flow and temperature fields for both cases. The geometric configuration of each cooling channel was optimised for Newtonian and non-Newtonian fluids, at a fixed inlet velocity and heat transfer rate. In addition, the effect of porosity on thermal conductance was investigated. Geometric optimisation was employed to the simple and complex microchannels, whereby an optimal geometric ratio (height versus length) was found to maximise thermal conductance. Moreover, analysis indicated that the bifurcation point of the complex microchannel could be manipulated to achieve a higher thermal conductance. In both cases, it was found that the non-Newtonian fluid characteristics resulted in a significant variation in thermal conductance as inlet velocity was increased. The ii characteristics of a dilatant fluid greatly reduced thermal conductance on account of shear-thickening on the boundary surface. In contrast, a pseudoplastic fluid showed increased thermal conductance. A comparison of the simple and complex microchannel showed an improved thermal conductance resulting from greater flow access to the conductive area, achieved by the complex microchannel. Therefore, it could be concluded that a complex microchannel, in combination with a pseudoplastic working fluid, substantially increased the thermal conductance and efficiency, as opposed to a conventional methodology. / Dissertation (MEng)--University of Pretoria, 2012. / gm2014 / Mechanical and Aeronautical Engineering / unrestricted

Knowledge of the leftventricular (LV) pressure-volume relation, along withparameters derived from this relation, have led to newpossibilities for the characterisation of cardiac pumpfunction, in both experimental studies and clinicalsettings. The pressure-volume diagram is apowerful tool for visualising LV performance, but in order tobe clinically useful it is necessary to make plots continuouslyand on-line. The conductance catheter technique offers thispossibility. The conductance catheter system has experiencedgrowing interest among cardiologists, physiologists, surgeons,and anaesthesiologists around the world as a powerful newresearch tool, but the invasiveness of this technique has beena limiting factor for most clinical applications. The catheterneeds to be thinner and softer in order to make this techniquemore suitable for human use. This thesis reports of a newthin and soft conductance catheter for continuously and on-linemeasurements of LV pressure and volume. One way to reduce both cathetersize and stiffness is to decrease the number of electrodes onthe catheter. Theoretical calculations shown in this thesisproves that it is possible to obtain the same performance witha single segment catheter as with a five-segment catheter. Thethin catheter has been tested and compared to a commercialfive-segment conductance catheter in animal studies. We conclude that the thin singlesegment conductance catheter can measure left ventricularvolume and pessure. The regression coefficient between the twomethods is good independent of loading condition and duringbaseline conditions the catheters produce very similar volumecurves. During preload reduction the estimated volume reductionis different in the two systems. Our thin catheter does notdisturb the heart's normal electrophysiology, neither by thecatheter current nor by any mechanical stimuli. The resultsdemonstrates that our thin, soft, single segment conductancecatheter has performance characteristics which warrant furtherdevelopment, with the goal to make the method available forhuman use. / NR 20140805

People with aphasia are known to have poor word retrieval abilities in communicative tasks. It has also been reported that they have lower, non-optimal levels of physiological arousal, which may cause lower attention levels therefore contributing to poor performance on linguistic tasks. The purpose of this study was to investigate the relationship between physiological arousal and word retrieval in adults with aphasia and neurotypical adults when presented with emotional stimuli within a confrontational naming task. Participants included 6 people with aphasia and 15 neurotypical controls. All participants completed a confrontational naming task within 3 emotional conditions (neutral, positive, negative) and physiological measures (Heart Rate Variability, Skin Conductance) were taken simultaneously. No statistically significant results were found; however, numerical trends were identified in the data that may provide direction when designing future studies.

The prevalence of cardiovascular disease in the United States dramatically increases with age. A hallmark feature of the aged myocardium is increased fibrosis resulting in diastolic dysfunction. Moreover, the survival of patients subsequent to a myocardial infarction is inversely related to age because of a certain extent to maladaptive remodeling mediated by cardiac fibroblasts. Our hypothesis is that cardiac fibroblast (CF) dysfunction results in overexpressed TGF-β1 leading to increased cardiac collagen content in the aged population. TGF-β1 stimulates the synthesis of the extracellular matrix proteins, including collagen in the cardiac tissues. The RT-PCR analysis of mRNA expression of TGF-β1 of the CF was increased by 43% in the aged mice as compared to the younger. The stiffness of the left ventricle is expressed with the slope of the end-diastolic pressure-volume relationship parameter, β (mmHg/μL). In a mouse model, we demonstrated that β was 0.30 ± 0.05 in the young as compared to 0.52 ± 0.10 in the aged (p < .05). The ventricular stiffness was associated with the myocardial collagen content; namely, young versus the aged was 9.5 ± 4.0 as compared to 16.4 ± 2.3% of total protein, respectively (p < .05). In conclusion, the gene structure-function relationships support our hypothesis that cardiac fibroblast disregulation contributes to diastolic filling dysfunction in elderly persons. These data provide a potential contributory mechanism for diastolic dysfunction that may be vital in caring for the aged open-heart surgical patient.