<p>Härtill 5 uppsatser.</p> / digitalisering@umu

The diploma thesis "The Picture of Brazil in Czech Media" focuses on finding out and defining of how the Czech media reprezented by four national dailies Hospodářské noviny, Lidové noviny, Mladá fronta DNES and Právo inform about Brazil. The researched sample are texts about Brazil that the four dailies have published during the year 2011. All 1635 texts that were published in this year were subjected to content analysis. By using quantitative content analysis, this thesis identifies the frequency of the publishing of texts about Brazil, their topical focus, lenght or placement within the newspaper. Other researched phenomena are sources, division according to genre, news and commentary or hard news and soft news. The character and number of texts focusing on both countries testifies to the possible closeness of Brazil and the Czech Republic. The thesis places special focus on the evaluation of Brazil in individual texts. The qualitative analysis mainly researches the possible elements of stereotyping in the language of Czech media. The results of these analyses are ilustrated further by the results of a questionnaire research. Comparison of the answers of respondents in the questionnaire and the results of the analyses testifies to whether the way the public perceives Brazil is the same or...

Os microRNAs (miRs) desempenham um papel importante na biologia das células-tronco por meio da interação com seus mRNAs alvos, induzindo inibição da tradução e/ou degradação destes transcritos. Durante a diferenciação de células pluripotentes, os miRs podem ser induzidos ou reprimidos, no entanto, suas funções específicas são amplamente inexploradas. Nós investigamos os papéis funcionais de um conjunto selecionado de miRs na pluripotência e diferenciação celular, usando microscopia de fluorescência quantitativa (High Content Analysis). Para isso, foram empregadas a NTera-2 (células de carcinoma embrionário humano, CCE) e a H1 (células-tronco embrionárias humanas, CTEh) como modelos. Essas células foram transfectadas reversamente com trinta moléculas de miRs distintas (individualmente) ou moléculas controles. Após 3-4 dias de cultura, as células foram fixadas, permeabilizadas e coradas com Hoechst / CellMask Blue (núcleo/citoplasma), anti-OCT4, anti-Ciclina B1 e imageadas com um sistema ImageXpress Micro HCA. O CellProfiler foi utilizado para quantificar vários parâmetros morfométricos e medidas de intensidade de OCT4 e Ciclina B1 em compartimentos nucleares e citoplasmáticos. Esses dados foram usados para gerar perfis fenotípicos multiparamétricos específicos de cada miR (usando KNIME) e o agrupamento desses dados levou à identificação de vias e processos envolvidos na indução de características de pluripotência ou diferenciação celular causadas por miRs com efeitos fenotípicos similares. Como exemplo, as vias de PI3K-AKT, WNT, TGF? e DICER foram encontradas como moduladas por alguns clusters fenotípicos e os transcritos de alguns alvos foram avaliados por qPCR para validar os achados. Parte do trabalho foi focada na regulação da via Notch por miRNAs em células pluripotentes, o que levou à observação de que o miR- 363-3p inibe a sinalização de Notch e promove pluripotência nessas células. A transfecção de miR-363-3p não apenas elevou as características de pluripotência em NTera-2 e H1, mas também protegeu as CCE da diferenciação induzida por cocultivo com OP9 expressando DLL1 e causou a diminuição no nível de transcritos de PSEN1. Em conclusão, o ensaio desenvolvido aqui provou ser uma ferramenta robusta na detecção de mecanismos moleculares, baseando-se na combinação de análises fenotípicas funcionais e bioinformáticas. / microRNAs (miRs) play an important role in stem cell\'s biology by binding to target mRNAs transcripts, inducing translation blockage and/or transcripts degradation. Upon differentiation of pluripotent cells, miRNAs can be induced or repressed, however, their specific roles are largely unexplored. We investigated the functional roles of a selected set of miRs in pluripotency and differentiation, using quantitative automated fluorescence microscopy (High Content Analysis). For this, we used NTera-2 (human embryonal carcinoma cells, ECC) and H1 (embryonic stem cells; ESC) as models. These cells were reverse-transfected with thirty distinct miRs mimics (individually) or control molecules. Following 3-4 days of culture, cells were fixed, permeabilized and stained with Hoechst/CellMask Blue (nucleus/cytoplasm), antiOCT4, anti-Cyclin B1 and imaged using an ImageXpress Micro HCA System. CellProfiler was used to quantify several morphometric parameters and intensity measurements of OCT4 and CYCB1 in nuclear and cytoplasmic compartments. Quantified parameters were used to generate miR-specific multiparametric phenotypic profiles (using KNIME) and clustering these data led to identification of pathways and processes involved in the induction of pluripotency or cell diferention features caused by miRs with similar phenotypic effects. As an example, PI3K-AKT, WNT, TGF? and DICER pathways were found to be regulated by some phenotypic clusters and transcripts level of some of miR targets were evaluated by qPCR to validate de findings. Part of the work was focused in the regulation of Notch pathway by miRNAs in pluripotent cells, which led the observation that miR-363-3p inhibits Notch signaling and promotes pluripotency feature, as the transfection with miR-363-3p mimic not only enhanced pluripotent phenotype in NTera-2 and H1, but also protected de ECCs from differentiation induced by coculture with OP9 expressing DLL1 and decreased PSEN1 transcripts level.In conclusion, The assay developed here proved to be a robust tool in the detection of molecular mechanisms based on combined functional phenotypic and bioinformatic analyzes.

More research is needed to fully understand the way in which parents, particularly fathers, are portrayed in family films and the effects those portrayals might have. Viewers, particularly parents, need to understand how the material their children view presents reality and how it may shape their children's perspectives of the real world, particularly where the family unit and parenting role are concerned. By exploring these portrayals through the lens of Cultivation Theory, this study sought to answer this overarching question: How are fathers portrayed in family films as opposed to television? This quantitative study explores the top twenty films from the 1980s, the 1990s and the decade spanning 2004-2014 in order to ascertain this. Families within those films, particularly parents and most specifically fathers, are the primary subjects of study. Observations were made through content analysis. The findings show that fathers are portrayed more positively in family films than they are on television. The data suggest that the differences between fathers and mothers in film are not so marked and sexist as they are in television shows, and that fathers may exert a stronger and more positive role in film families than they do in television families. This study begins to establish film as a genre to further be explored as a medium for family relations studies as television has been. Film is a powerful media tool in its own right and should further be studied with regard to portrayal of families in its material.

Os microRNAs (miRs) desempenham um papel importante na biologia das células-tronco por meio da interação com seus mRNAs alvos, induzindo inibição da tradução e/ou degradação destes transcritos. Durante a diferenciação de células pluripotentes, os miRs podem ser induzidos ou reprimidos, no entanto, suas funções específicas são amplamente inexploradas. Nós investigamos os papéis funcionais de um conjunto selecionado de miRs na pluripotência e diferenciação celular, usando microscopia de fluorescência quantitativa (High Content Analysis). Para isso, foram empregadas a NTera-2 (células de carcinoma embrionário humano, CCE) e a H1 (células-tronco embrionárias humanas, CTEh) como modelos. Essas células foram transfectadas reversamente com trinta moléculas de miRs distintas (individualmente) ou moléculas controles. Após 3-4 dias de cultura, as células foram fixadas, permeabilizadas e coradas com Hoechst / CellMask Blue (núcleo/citoplasma), anti-OCT4, anti-Ciclina B1 e imageadas com um sistema ImageXpress Micro HCA. O CellProfiler foi utilizado para quantificar vários parâmetros morfométricos e medidas de intensidade de OCT4 e Ciclina B1 em compartimentos nucleares e citoplasmáticos. Esses dados foram usados para gerar perfis fenotípicos multiparamétricos específicos de cada miR (usando KNIME) e o agrupamento desses dados levou à identificação de vias e processos envolvidos na indução de características de pluripotência ou diferenciação celular causadas por miRs com efeitos fenotípicos similares. Como exemplo, as vias de PI3K-AKT, WNT, TGF? e DICER foram encontradas como moduladas por alguns clusters fenotípicos e os transcritos de alguns alvos foram avaliados por qPCR para validar os achados. Parte do trabalho foi focada na regulação da via Notch por miRNAs em células pluripotentes, o que levou à observação de que o miR- 363-3p inibe a sinalização de Notch e promove pluripotência nessas células. A transfecção de miR-363-3p não apenas elevou as características de pluripotência em NTera-2 e H1, mas também protegeu as CCE da diferenciação induzida por cocultivo com OP9 expressando DLL1 e causou a diminuição no nível de transcritos de PSEN1. Em conclusão, o ensaio desenvolvido aqui provou ser uma ferramenta robusta na detecção de mecanismos moleculares, baseando-se na combinação de análises fenotípicas funcionais e bioinformáticas. / microRNAs (miRs) play an important role in stem cell\'s biology by binding to target mRNAs transcripts, inducing translation blockage and/or transcripts degradation. Upon differentiation of pluripotent cells, miRNAs can be induced or repressed, however, their specific roles are largely unexplored. We investigated the functional roles of a selected set of miRs in pluripotency and differentiation, using quantitative automated fluorescence microscopy (High Content Analysis). For this, we used NTera-2 (human embryonal carcinoma cells, ECC) and H1 (embryonic stem cells; ESC) as models. These cells were reverse-transfected with thirty distinct miRs mimics (individually) or control molecules. Following 3-4 days of culture, cells were fixed, permeabilized and stained with Hoechst/CellMask Blue (nucleus/cytoplasm), antiOCT4, anti-Cyclin B1 and imaged using an ImageXpress Micro HCA System. CellProfiler was used to quantify several morphometric parameters and intensity measurements of OCT4 and CYCB1 in nuclear and cytoplasmic compartments. Quantified parameters were used to generate miR-specific multiparametric phenotypic profiles (using KNIME) and clustering these data led to identification of pathways and processes involved in the induction of pluripotency or cell diferention features caused by miRs with similar phenotypic effects. As an example, PI3K-AKT, WNT, TGF? and DICER pathways were found to be regulated by some phenotypic clusters and transcripts level of some of miR targets were evaluated by qPCR to validate de findings. Part of the work was focused in the regulation of Notch pathway by miRNAs in pluripotent cells, which led the observation that miR-363-3p inhibits Notch signaling and promotes pluripotency feature, as the transfection with miR-363-3p mimic not only enhanced pluripotent phenotype in NTera-2 and H1, but also protected de ECCs from differentiation induced by coculture with OP9 expressing DLL1 and decreased PSEN1 transcripts level.In conclusion, The assay developed here proved to be a robust tool in the detection of molecular mechanisms based on combined functional phenotypic and bioinformatic analyzes.

This Thesis are dealing with analysis of political satire change during election periods in 2006 and 2010 in printed media (Právo, Lidové noviny and MF DNES). 1796 items were evaluated. In these media three artist in both years were publishing: in MF DNES it is Miroslav Kemel, in Lidové noviny Vladimír Jiránek and in Právo Vladimír Renčín. For political satire research content analysis was used, combining quantitative and qualitative methods. Defined variables are used for grouping images. The research should accept/reject five hypothesis directed to topic frequencies (domestic, foreign, non-political), politicans shown as the target of satire and political tending of particular paper. The most popular target for political satire was Jiří Paroubek former leader of ČSSD due to his nature and physiognomy. This was true for MF Dnes and Lidové noviny. In Právo no concrete politican was drawn by V. Renčín in both years. In the first place topic Election was in 2006 in all papers, in 2010 only for MF Dnes it was Nr 1. In other paper most important topics were morality and demenaurs of politicans. This Thesis confirmed that left-winged paper Právo is not tending to the righ-wing ridiculing. On the contrary, the other newspapers, Lidové noviny and MF Dnes, both being right-wing newspapers, published the...

Thesis Lifestyle in magazine Květy (1960 - 1968) deals with the lifestyle in the years 1960 - 1968 in Czechoslovakia from the viewpoint magazíne Květy. The work examines how the magazine, presented lifestyle in the reference period and how they influenced the ideas of readers' lifestyles. Due to the great range of lifestyle related work has focused solely on housing, leisure and fashion. In the field of housing, work has concentrated on available housing, household furniture, decorations and articles of daily use. In the fashion most attention was focused on the clothing, hairstyles then in fashion and beauty treatments. Leisure time has been reduced to activities designed for relaxation, entertainment and distraction, prevailed in the area of recreation, sports activities and activities of various interest groups. Lifestyle in the analysis of the text was analyzed using a combination of qualitative and quantitative methods of content analysis. Quantitative analysis is mainly concerned with the development of a range of lifestyle topics. Qualitative analysis provides information about the content of a particular lifestyle topics. The paper also deals with the theoretical embedding the concept of lifestyle, a lifestyle of Czechoslovakia in the 60s 20th century, the magazine examined the...

AbstraktBakgrund: Den specialiserade palliativa hemsjukvården har ökat senaste åren. För engod och trygg vård är det viktigt att mötet med patienten i palliativ vård blirtillgodosedd och att närstående känner en delaktighet. Empati är kärnan försjuksköterskan i palliativ vård och att ha en förmåga att sätta sig in i patientens situationoch med det uppnå professionellt förhållningsätt. Sjuksköterskans uppgift är atttillgodose patientens och dennes närstående fysiska, psykiska, sociala och existentiellabehov.Syfte: Syfte är att belysa de upplevelser och erfarenheter sjuksköterskor ihemsjukvården har av att vårda unga vuxna och medelålders patienter i palliativ vård.Metod: Semistrukturerade intervjuer genomfördes med åtta sjuksköterskor ochdistriktssköterskor som arbetade i kommunal hemsjukvård. Datamaterialet analyseradesmed kvalitativ innehållsanalys .Resultat: Analysen resulterade i fem kategorier; egna känslor, närstående, det svårasamtalet och mötet, yrkesrollens förutsättningar och arbetstillfredsställelse.Otillräcklighet, orättvisa, osäkerhet, sorg och rädsla var känslor som sjuksköterskor ochdistriktssköterskor upplevde i mötet med den yngre patienten och dennes närstående ipalliativ vård. Sjuksköterskan upplevde det viktigt att ha självinsikt vid samtal somberör existentiella frågor och för att kunna initiera till samtalet krävs erfarenhet. För attkunna ge en god och trygg vård till familjen krävs det professionellt förhållningsättgenom att visa trygghet, visa medkännande, vara lyhörda och orka stötta. För att orkamed detta och kunna visa kraft och styrka är reflektion och ett gott samarbete medkollegorna av stor vikt.Slutsats: Reflektion, handledning och utbildning var viktigt för sjuksköterskan i arbetetmed den yngre patienten i palliativ vård. Sjuksköterskorna beskrev arbetet med denyngre patienten i palliativ vård och dennes närstående som meningsfullt och att detväcker engagemang och är utvecklande.Nyckelord: Hemsjukvård, kvalitativ innehållsanalys, palliativ vård, sjuksköterskansupplevelse / AbstractBackground: The advanced palliative home-care has been increasing the last few years. For a good and safe care it´s important that the encounter with the patient is satisfying and the relatives feel participation. Empathy is the core to the nurse in palliative care and having an ability to familiarize themselves with the patient´s situation and with it achieve a professional approach. The nurse´s role is to cater to the physical, psychological, social and existential needs of the patient and relatives.Purpose: The purpose of the study was to illuminate the registered nurses´ experiences of care of young adults and middle-aged patients in palliative care.Method: Semi-structured interviews where completed with eight nurses and district nurses. The nurses worked in palliative home-care. The analysis was conducted using a qualitative content analysis.Results: The analysis resulted in five categories; own feelings, relatives, the difficult conversation and encounter, prerequisites for the professional role and work satisfaction. Inadequacy, injustice, uncertainty, sorrow and fear turned out to be feelings registered nurses and district nurses experienced in the meeting with the younger patient in palliative care and the relatives. The nurse experienced it to be important to have self-awareness whilst conducting conversations with existential questions and to be able to initiate conversations was experience required. To be able to give a good and safe care to the family is a professional approach necessary by giving security, be compassionate, responsive and cope to give support. To be able to cope this and show force and strength is reflection and good teamwork with the colleagues of great importance.Conclusion: Reflection, guidance and education were factors of importance for the nurses´ care of the younger palliative patient. The nurses described their work with the patient and relatives as meaningful, engaging and developing.Key words: Content analysis, home-care, palliative care, qualitative content analysis the nurse´s experience.

Structural changes in the knowledge economy have greatly affected the way business is conducted and the processes firms create value. The financial reporting system is inadequate as a result of such changes, and disclosure of intellectual capital (IC) information has gained importance for communicating with capital markets. Empirical research documents corporate governance (CG) factors influencing IC disclosure practices, as well as demonstrates the value-relevance and predictive power of IC information. The disclosure of IC information by listed firms is a topic that has attracted considerable attention from contemporary researchers, but scant empirical evidence exists. Much of the researchers has examined CG as a key determinant of IC (and nonfinancial) disclosure; in contrast, few provides evidence for explaining their controversial findings of board independence on disclosure. In addition, a lack of studies confirms the literature about the use of IC information by capital market participants. Therefore, this thesis aims to examine disclosure of IC information in relation to outside directors, auditors, and sell-side analysts respectively. The specific objectives of this thesis are to examine whether outside directors’ expertise is a determinant of IC disclosure; and the extent to which the disclosure of IC information impacts on audit effort and analysts’ forecasts. In order to address these research objectives, a content analysis of IC disclosure (a self-constructed index of 64 coded items) in strategic reports released by FTST 350 companies is used. The content analysis captures and measures IC disclosure by category (i.e., human, structural & relational capital), notion (i.e., static vs. dynamic), and connection (i.e., across categories vs. with strategies). Using multivariate regression models that were primarily developed upon information asymmetry arguments and agency theory, the specific objectives of this thesis are addressed in three empirical chapters. The findings in Chapter 3 showed that proportion of outside directors (NEDs) with cross-directorship, nonaccounting and academia expertise has a positive association with IC disclosures, whereas board independence itself has no effect on the disclosures. The findings indicates that the monitoring role of NEDs alone is inadequate in promoting IC disclosure. Rather, it supports the importance of the dual role (i.e., monitor and advisory) of a supervisory board. The results also respond to the UK CG Code in their recommendation that the combination of skills, experience and knowledge guarantees a sound information environment to the market. Nonetheless, findings raised a further concern about the quantity of IC disclosures when companies have more NEDs with accounting expertise. On whether and how disclosure of IC information impacts on audit effort, Chapter 4 found that firms with high levels of IC disclosure in the previous year pay more audit fees (proxied for audit effort) in the current year regardless of their earnings quality conditions. It was also found that firms greatly disclosing dynamic IC information are charged more than those of focusing on static IC disclosure. In addition, findings in Chapter 5 revealed that there is a negative relation between IC disclosure and analyst forecast errors, indicating that UK sell-side analysts appreciate the disclosure of IC information and thus confirming that IC information has predictive ability of explaining a firm’s future value. It was further identified that disclosed IC information absorbs the negative effect of concentrated executive ownership and opaque financial environment. Overall, the results of this thesis suggest that IC reporting process could be improved by having sufficient outside directors with certain types of expertise on the board. In doing so, improved IC disclosure helps to reduce information asymmetry (proxied by analyst forecast accuracy) between firms and outside investors, albeit firms bear a significant increase in audit fees. This study calls for guidelines for IC disclosure in the UK and the support of assurance services to enhance credibility of firm-provided IC information in a bid to promote the communication of IC information with the capital market.

Sjuksköterskor verksamma på barnavårdscentral (BVC) möter dagligen flyktingbarn och deras föräldrar i sitt arbete och dessa möten ser olika ut beroende på olika faktorer. Syftet med studien var att beskriva BVC- sjuksköterskors arbete med flyktingbarn och deras föräldrar. Semistrukturerade intervjuer genomfördes och analyserades med kvalitativ innehållsanalys. Ur resultatet kom tre kategorier: att skapa trygghet, att vara lyhörd och att ge stöd. I kategorin att skapa trygghet beskrev BVC- sjuksköterskor hur de arbetade för att skapa jämlik vård, öka tillgängligheten samt belyste de vikten av kontinuitet i vården för flyktingbarnen. Kategorin att vara lyhörd innefattade sjuksköterskors sätt att se det enskilda barnets behov. Detta tillämpades med respekt, förståelse och acceptans för kulturella skillnader. Att ge stöd genom behjälplighet vid språk-och kommunikationssvårigheter, rätt anpassad information och samverkan med andra professioner gav flyktingbarnen tryggare och snabbare integrering i det svenska samhället. Svårigheter som BVC- sjuksköterskorna beskrev var kommunikation via tolk, brist på resurser och brist på kontinuitet i arbete med flyktingbarn. Fortsatt forskning behövs för att få ökad kunskap och förståelse kring flyktingbarns behov. Det behövs även fortsatta studier i utveckling, organisation och samordning av verksamheter som bedriver vård och omsorg för flyktingbarn. / Nurses working in Child Health Centers (CHC-nurses) face daily refugee children and their parents in their work and these meetings vary depending on various factors.The aim of this study was to describe CHC-nurses experience of working with refugee children and their parents. Semi-structured interviews were conducted and were analyzed using qualitative content analysis. From the result three categories emerged; to create security, to be responsive and to provide support. In the category to create security the CHC-nurses described how they worked to create equal care, increase accessibility, and highlight the importance of the continuity in the health care of refugee children. Category to be responsive comprised nurses' approach to the individual child's needs which was applied with respect, understanding and acceptance of cultural differences. Providing support through helpfulness with language and communication difficulties, the right customized information and collaboration with other professions gave refugee children safer and faster integration into the Swedish society. Difficulties described by the CHC-nurses were communication through interpreters, lack of resources and lack of continuity in the work with refugee children. Continued research is needed to increase knowledge and understanding of refugee children’s needs. Further studies in development, organization and coordination of activities engaged in health care for refugee children are needed.