The schedule of growth, reproduction and survivorship of an individual are the key components of life history, which reflect fitness performance of a genotype. Examining the variation in life history performance among genotypes provides an avenue for which genotypic fitness differences can be compared. The parthenogenetic freshwater zooplankton, Daphnia pulicaria, is a model organism to investigate and disentangle the genotypic from the phenotypic influences of life history variation. While Daphnia spp. life history has been extensively studied, few have examined the effect of low food concentrations on life history traits. Since Daphnia spp. are frequently subjected to periods food limitation caused by natural phytoplankton cycles, it is necessary to understand how individuals respond in low food environments in order to obtain an accurate representation of life history responses among genotypes. In this study, I conducted a set of highly controlled laboratory experiments using multiple genotypes of Daphnia pulicaria under a range of food-limited conditions in order to gain insight into the environmental and genotypic responses of life history traits. I measured a suite of life history traits, growth, reproduction and survivorship, as discrete elements for each individual and synthesized these traits into a representation of the life history schedule. This provided an accurate method to compare genotypes and allowed for identification of trade-offs between life history traits. My results indicate a significant effect of low food concentration on life history traits, causing a decrease in performance of all traits. Additionally, genotypic differences occurred in most traits, however these differences only manifested within the intermediate food levels. There were no genotypic differences in daily reproductive rate, indicating that genotypes only differ in the number of individuals reproducing within an environment. Allocation-based trade-offs among these life history traits were examined within a genotype, however the only trade-off identified was between reproduction and survivorship and this only manifested at low food levels. Even though these genotypes do not co-exist in the same lakes, similar patterns occurred in trade-offs among genotypes. This potentially indicates that trade-offs among genotypes occur due to the same physiological mechanisms witnessed at the individual level. / Thesis (Master, Biology) -- Queen's University, 2011-08-14 11:32:27.808

Parasites are thought to have large effects on their host populations, driving genetic change, population density changes, speciation and be a major selective force maintaining sexual reproduction. Indirect signatures of parasite-mediated selection are common, but explicit examples of parasite-mediated selection in nature are lacking. In this thesis I examine parasite-mediated dynamics in a natural population of Daphnia magna that experiences an annual epidemic of the bacterial pathogen Pasteuria ramosa. I also test a novel hypothesis investigating the relationship between parasitism and the production of resting eggs. In chapter 2 a combined field study and laboratory infection experiment illustrates one of the best examples of parasite-mediated selection in a natural population, with Daphnia collected after a parasite epidemic having higher levels of parasite resistance than those collected before. This chapter also explored the relationship between parasitism and resting eggs, which are only produced during the sexual phase of reproduction. Daphnia that were reproducing sexually in the field prior to the parasite epidemic were more susceptible, supporting higher levels of parasite growth, than their asexual counterparts. This supports the idea that some genotypes invest in sex at the expense of parasite resistance. In chapter 3 I used molecular markers to investigate genotype frequency changes in the same population in relation to the parasite epidemic. The parasite epidemic was found to be associated with genetic change in the population, and a laboratory infection experiment revealed that the genotype most resistant to the parasite was also most common following the peak of the parasite epidemic. While chapter 2 explored a genetic relationship between susceptibility and resting eggs, chapter 4 explores whether crowding conditions, cues indicating parasite prevalence in the population, or direct exposure to parasite spores can induce resting egg production. I found that crowding conditions or parasite prevalence enhance levels of male and resting egg production, but patterns were entirely dependent on Daphnia genotypes. There was no indication that exposure to parasite spores affects levels of sexual reproduction.

The current work determined whether there is a constant tissue residue associated with narcotic compounds. In this investigation, the cladoceran, Daphnia magna was exposed to lethal levels (48h LC50) of ten, $ sp{14}$C-labelled, narcotic organic chemicals in a closed system. Exposure times, ambient concentrations, and body sizes were varied to evaluate their effects. The $ sp{14}$C-method developed in current work can detect chemicals in single D. magna in concentrations ranging from 0.02 to 6310 mmol/kg. Moreover, the technique detected phobic and lipophilic chemicals equally well. The technique's sensitivity (nmol/kg) allowed for detection of differences in the internal concentrations of pollutants among the unaffected, immobilized, and dead D. magna. Immobilized D. magna contained between 0.14 mmol/kg and 200 mmol/kg of narcotics. On the average, however, the internal residues were 3.1 mmol/kg (95%CL = 3.1 $ pm$ 2.0). This agreed with literature values. The effects of time of exposure, ambient concentration, and body size on the tissue residues of narcotics varied with the chemical compound.

Chemical communication in aquatic organisms has been topic of a large number of studies focusing interactions between organisms via info chemicals. Diel Vertical Migration (DVM) is commonly observed among zooplankton and consists of a single daily ascent with minimum depth reached between sunset and sunrise and a descent with maximum depth attained during the day. DVM was absent or reduced when predators were absent and well developed in their presence. Species of the Daphnia are one of the well investigated group in freshwater environments. Variation in DVM of Daphnia in response to fish kairomone is one of the best studied behavioral strategies. Kairomone, as a term, is described interspecific chemical messengers, the adaptive benefit of which falls on the recipient rather than the emitter. As a result, nature and origin of kairomone is still unclear and needs to be investigated. It was decided that FT-IR technique would be favorable tool for this aim. In this frame, it was conceived that the occurrence of migration adaptation relevant to the seasonal changes in the presence of fish kairomone could be proved and characterized by FT-IR technique. Results of the present study indicate that non-aromatic, secondary amine compound has significant contribution to fish cue. Since other sources other than fish can contribute the natural amine compounds level in fresh water environment, origin and concentration of amines are needed further investigation to determine ecological function of amine.

Thesis (M.S.)--Baylor University, 2008. / Includes bibliographical references (p. 68-74)

Made available in DSpace on 2014-10-09T12:27:07Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T13:56:20Z (GMT). No. of bitstreams: 0 / Dissertacao (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Ambiental. / Made available in DSpace on 2012-10-23T12:56:28Z (GMT). No. of bitstreams: 1 243670.pdf: 1308460 bytes, checksum: 0f6c64928676bb28c3f55aa6e38b5498 (MD5) / Resíduos sólidos industriais necessitam de tratamento e disposição final adequada. Para que isto seja feito corretamente, cada resíduo deve ser previamente classificado em Classe I ou Classe II. Este método de classificação é proposto pela NBR 10004/04, contudo, é complexo e demanda tempo. Com o objetivo de facilitar a classificação, este trabalho propõe a utilização de ensaios com o microcrustáceo Daphnia magna para este fim. Os ensaios possibilitam a identificação de substancias tóxicas no lixiviado, o que denota a presença de uma das características descritas pela norma, a toxicidade, que é argumento suficiente para classificar o resíduo em Classe I. Realizaram-se ensaios ecotoxicológicos com trinta e duas amostras de resíduos sólidos industriais e, com base nos resultados das CE50(%) 48h observadas nestas amostras em comparação com a classificação oficial da NBR 10004/04, estabeleceram-se limites para classificação dos resíduos em Classe I ou Classe II. Verificou-se coincidência na classificação de 62,5% das amostras analisadas. Nos casos em que não foi observada coerência entre os métodos, o método proposto neste trabalho frequentemente classificou o resíduo como Classe I. Os dados obtidos revelaram que o método de classificação proposto é promissor pela sua rapidez e eficiência na preservação do meio ambiente.

Neste trabalho foi avaliado o desempenho do sistema de contatores biológicos rotatórios para a remoção de matéria orgânica de efluente hospitalar e a posterior inativação de coliformes totais, Escherichia coli e Enterococcus sp. com os oxidantes hipoclorito de sódio e ozônio. A toxicidade gerada nos processos de desinfecção foi avaliada em Daphnia similis. Os efluentes hospitalares podem apresentar semelhanças aos efluentes domésticos no que diz respeito à concentração de matéria orgânica, coliformes e pH e ambos são, geralmente, coletados pela rede de esgotos e enviados para mesma estação de tratamento. Contudo, a presença de substâncias como fármacos, desinfetantes e compostos químicos, bem como organismos patogênicos multirresistentes a antimicrobianos podem ocorrer em elevadas contagens nas águas residuárias hospitalares. O sistema biológico de tratamento utilizado nesta pesquisa se mostrou adequado, obtendo-se remoções de matéria orgânica na ordem de 80% em termos de DQO, sendo que no término do experimento atingiu-se 88,5% de remoção de DQO para o tempo de detenção hidráulico de 2,28 horas. Houve a inativação de 1 a 2 unidades logarítmicas para coliformes totais, de 2 a 3 unidades logarítmicas para Escherichia coli e significativa remoção de toxicidade. O desempenho dos desinfetantes hipoclorito de sódio e ozônio mostrou similaridades. Entretanto, ao comparar-se com efluentes domésticos, foram necessárias maiores dosagens para o efluente hospitalar devido ao elevado consumo de oxidante pela presença da matéria orgânica refratária ao tratamento biológico. O processo de desinfecção com hipoclorito de sódio apresentou variabilidade nos resultados, em função da concentração de matéria orgânica, nitrogênio amoniacal e pH do efluente. Para ensaios em bateladas em volumes de amostra de 1 litro, valores de C.t oscilaram entre 20 a 50 mg.min.L-1 para inativação de E. coli. A desinfecção por ozônio também sofreu variações em função da matriz complexa do efluente. A inativação dos organismos somente foi acentuada após adição de 70 a 90 mg.L-1 de ozônio, concentrações estas nas quais se observou grande decaimento na absorbância em UV 254 nm, indicando possivelmente o consumo prioritário de ozônio para outras reações de oxidação, tais como a ruptura de anéis aromáticos e/ou insaturações nas cadeias carbônicas. Efluentes de origem doméstica foram rapidamente desinfetados com ozônio. Os organismos Enterococcus sp. apresentaram decaimento ora semelhante a coliformes totais, ora semelhante a Escherichia coli e ora resistentes frente à ação do desinfetante. Após total inativação de coliformes fecais foram eventua lmente observadas contagens na ordem de 103 UFC.100mL-1 de Enterococcus sp. Dada a prevalência de organismos resistentes a antibióticos que, em função disso, apresentam maior grau de virulência, observou-se que somente o monitoramento de coliformes totais e fecais não seria adequado para a desinfecção e lançamento de efluentes hospitalares. A toxicidade aguda do efluente, verificada em Daphnia similis, aumentou após adição de cloro mas foi reduzida quando houve decloração com tiossulfato de sódio. Com adição de ozônio, verificou-se variabilidade nos resultados, mas geralmente houve aumento da toxicidade após aplicação de elevadas dosagens as quais foram necessárias para desinfecção.

The impact of fish predation on zooplankton assemblage was studied in the mesocosm experiment. Presented thesis focuses on the effect of size selective fish predation on species diversity, biomass and body sizes of zooplankton and the impact of fish occurrence on zooplankton behaviour with regard to diel horizontal migration. I also studied a change in some life history traits of zooplankton caused by fish with a special focus on keystone species of freshwater ecosystems Daphnia spp.

Atrazine photochemical degradation in homogeneous phase using Fe(III)/UV/air system was studied. Two toxicity assessments, a Lemna minor growth inhibition test and a Daphnia magna acute immobilisation test, were employed to test potential toxicity of atrazine and its degradation products. The occurrence of atrazine in rivers from the Vltava River basin was evaluated from the analyses performed by Povodí Vltavy, State Enterprise.