The data modeling phase is one of the main phases in automated system development methods. Usually data modeling is separated as independent process which success and robustness depends largely on the intuition and experience of the data analyst. This can become problematic when IS database is being designed for large scale IS. To solve this type of problems it’s proposed to accomplish data modeling phase not as a separate, poorly with the generic modeling process connected part but as one of the fundamental system development phases. Functional Requirements Specification Method is proposed for this matter. This methods main idea is to build database model according to user requirements for the functionality of the system being developed. The purpose of this work is to present a process intended for building a entity relationship diagram from the analysed functional requirements specification metamodel and generation of the SQL DDL script (relational schema) from that model. The presented model will be implemented as one of the CASE tool modules specified for the database modeling and SQL DDL script generation. Implemented tool will be verified and it’s correctness will be experimentally approved.

The purpose of this master's project was to write a resource book that demonstrates how teachers can use data-driven learning methods to teach vocabulary. First, a brief overview of corpus linguistics, data-driven learning, and the corpus used in this book (COCA) is given. Then, the book presents different aspects of vocabulary learning in the context of a corpus. Topics included are frequency knowledge, part of speech knowledge, morphological knowledge, synonym knowledge, collocational knowledge, and register knowledge with a chapter on each topic. For each aspect of vocabulary learning there is a section that introduces the topic to the teacher, followed by instructions on performing topic related searches in the corpus. Each chapter also includes examples and ideas for application to the vocabulary classroom. Additional chapters provide information on individual language learning, and an evaluation of the project. The goal of this project was to provide teachers with specific knowledge of vocabulary and corpus-linguistics to be able to teach less-frequently addressed aspects of vocabulary instruction and to encourage more use of corpora in the language classroom. It is hoped that after reading this book, teachers will be able to improve their vocabulary teaching and ability to use the Corpus of Contemporary American English and DDL methods in the ESL/EFL classroom. The evaluation of this project will consist of teacher reviews of the book after reading. Specifically, the questionnaire addresses readers' feelings of increased knowledge and understanding of these areas and desire to use them in the classroom.

The Dawdle (DDL) gene in Arabidopsis encodes a protein with a Fork Head-Associated domain. A mutation in the DDL gene causes pleiotropic phenotypes and reduced the levels of several microRNAs. However, it is not completely known whether the FHA domain of the DDL is necessary for its function. Furthermore, the interactors involved in the same signaling pathway as DDL have not been identified yet. To determine the role of FHA domain in DDL function, four Tiller mutant lines were isolated and characterized. Results from phenotypic characterization suggest that the FHA domain may require for DDL function. For interactors, the interactions between DDL and At1G68010, At2G28200, At3G68010 and At4G18372 were identified by Yeast Two Hybrid, and then confirmed by the Split Luciferase assays. In addition, T-DNA mutants for each interactor were isolated and analyzed phenotypically suggesting that the genes carrying those T-DNAs should function in the same signaling pathway as DDL.

A serina/treonina fosfatase do tipo 1 (PP1) é uma enzima ubíqua nas células e nos tecidos das várias espécies em que foi pesquisada e regula vários processos como metabolismo intermediário, processamento de mRNA, transcrição e apoptose. Geralmente a holoenzima PP1 é encontrada como um dímero constituído por uma subunidade catalítica conservada (PP1c) e uma ou mais subunidades reguladoras variáveis. Em mamíferos, já foram identificados mais de 50 polipeptídeos que se associam direta ou indiretamente a PP1c, gerando holoenzimas com localizações celulares e especificidades distintas. Entre estas proteínas estão inibidores citosólicos de PP1c, tais como o inibidor-1 (I-1), o inibidor-2 (I-2) e o inibidor nuclear da PP1 (NIPP-1). Ortólogos do I-2 foram descritos em microorganismos como Saccharomyces cerevisiae e Neurospora crassa. Neste trabalho nós demonstramos que o genoma da ameba social Dictyostelium discoideum possui uma única cópia do gene que codifica um ortólogo do I-2 (DdI-2). Análise através de Northern blot mostrou que o mRNA de DdI-2 é expresso durante o crescimento e ao longo de todo o ciclo de desenvolvimento, com níveis variáveis. Também demonstramos que o gene DdI-2 codifica uma verdadeira proteína inibidora da PP1c uma vez que seu produto recombinante em bactéria é capaz de inibir, com eficácia equivalente, as atividades de fosforilase fosfatase das PP1c recombinantes selvagem (DdPP1c) e mutante (DdPP1cF269C) de D. discoideum e NcPP1c de N. crassa in vitro. A proteína DdPP1cF269C apresenta características distintas da DdPP1c incluindo maior estabilidade, maior atividade de fosforilase fosfatase e maior sensibilidade frente ao inibidor caliculina A. Estas diferenças devem-se a substituição da cisteína conservada da posição 269 por uma fenilalanina, que é verificada na enzima selvagem. DdPP1c e DdPP1cF269C foram também ensaiadas na presença de INc-1L e INc-1 que são ortólogos de I-2 em N. crassa. Ambas as proteínas recombinantes purificadas exibiram efeito inibidor sobre a atividade de fosforilase fosfatase das DdPP1c recombinantes selvagem e mutante, sendo que INc-1 foi um inibidor duas vezes mais eficiente que INc-1L. Este efeito pode ser devido a um segmento de 38 aminoácidos codificado por um íntron em fase que é retido na isoforma INc-1L. Nossos dados indicam ainda que a mutação F269C não afetou a sensibilidade da DdPP1c recombinante a nenhum dos ortólogos de I-2 testados in vitro. Ensaios de duplo-híbrido utilizando a PP1c selvagem e mutante de D. discoideum (DdPP1c e DdPP1cF269C) e de N. crassa (NcPP1c) como iscas e DdI-2 como presa mostraram que estas proteínas interagiram in vivo. Quando a presa era o INc-1L ou INc-1 a interação ocorreu apenas com a NcPP1c, sendo mais forte no caso de INc-1. As regiões de DdI-2 envolvidas na interação física com a DdPP1c foram mapeadas através da expressão de proteínas truncadas no ensaio de duplo híbrido. Os experimentos apontaram que o carbóxi-terminal de ~100 aminoácidos não é essencial para a interação, mas que o somatório das diversas regiões responde pela integridade da interação. / The serine/threonine phosphatase of type-1 (PP1) is a ubiquous enzyme in the cells and tissues from several species studied and regulates numerous processes such as intermediate metabolism, mRNA splicing, transcription, and apoptosis. PP1 holoenzymes consist of a well-conserved catalytic subunit (PP1c) and one or more variable regulatory subunits. In mammals, more than fifty polypeptides that bind PP1c have been identified, originating holoenzymes with distinct cell locations and specificities. These proteins include cytosolic PP1c inhibitors such as inhibitor-1 (I-1), inhibitor-2 (I-2) and nuclear inhibitor of PP1 (NIPP-1). I-2 orthologs have also been described in Saccharomyces cerevisiae and Neurospora crassa. In the present work, we demonstrate that the genome of the social amoeba Dictyostelium discoideum has a single gene encoding for an I-2 ortholog (DdI-2). Northern blot analyses have shown that DdI-2 mRNA is expressed throughout Dictyostelium developmental cycle at variable levels. We also demonstrated that DdI-2 is a true PP1c inhibitor as its recombinant product is capable of inhibiting the phosphorylase phosphatase activity of wild-type PP1c (DdPP1c) and mutant (DdPP1cF269C) of D. discoideum and NcPP1c of N. crassa in vitro. DdPP1cF269C protein presents distint traits including higher stability, phosphorylase phosphatase activity and sensibility to calyculin A than the wild-type. These differences are originated from the replacement of a well conserved cisteine residue by a phenylalanine found in the wild-type. The wild-type and mutant DdPP1c have also been assayed in the presence of INc-1L and INc-1 which are orthologues to I-2 in N. crassa. Both purified recombinant proteins have shown inhibitory effects over phosphorylase phosphatase activities, with INc-1 being twice more potent than INc-1L. This might be due to the presence of an intron retention event in the latter that results in a insertion of 38 aminoacids. Our data also indicate that F269C mutation did not affect DdPP1c sensitivity to inhibition by all the three recombinant I-2 orthologues in vitro. Yeast two-hybrid assays using wild type (DdPP1c) and mutant (DdPP1cF269C) D. discoideum and N. crassa (NcPP1c) PP1c as preys and the putative inhibitor DdI-2 as a bait showed inequivocally that these proteins interacted in vivo. When the prey was INc-1 or INc-1L the interaction occured only with NcPP1c and was stronger with INc-1. The domains of DdI-2 involved in the interaction with DdPP1c were mapped by two-hybrid interaction assays with DdI-2 deleted mutants. These experiments have pointed out that the DdI-2 carboxi-terminus of ~100 aminoacids is not essential for the interaction but that the sum of all regions is responsible for the integrity of the interaction.

A serina/treonina fosfatase do tipo 1 (PP1) é uma enzima ubíqua nas células e nos tecidos das várias espécies em que foi pesquisada e regula vários processos como metabolismo intermediário, processamento de mRNA, transcrição e apoptose. Geralmente a holoenzima PP1 é encontrada como um dímero constituído por uma subunidade catalítica conservada (PP1c) e uma ou mais subunidades reguladoras variáveis. Em mamíferos, já foram identificados mais de 50 polipeptídeos que se associam direta ou indiretamente a PP1c, gerando holoenzimas com localizações celulares e especificidades distintas. Entre estas proteínas estão inibidores citosólicos de PP1c, tais como o inibidor-1 (I-1), o inibidor-2 (I-2) e o inibidor nuclear da PP1 (NIPP-1). Ortólogos do I-2 foram descritos em microorganismos como Saccharomyces cerevisiae e Neurospora crassa. Neste trabalho nós demonstramos que o genoma da ameba social Dictyostelium discoideum possui uma única cópia do gene que codifica um ortólogo do I-2 (DdI-2). Análise através de Northern blot mostrou que o mRNA de DdI-2 é expresso durante o crescimento e ao longo de todo o ciclo de desenvolvimento, com níveis variáveis. Também demonstramos que o gene DdI-2 codifica uma verdadeira proteína inibidora da PP1c uma vez que seu produto recombinante em bactéria é capaz de inibir, com eficácia equivalente, as atividades de fosforilase fosfatase das PP1c recombinantes selvagem (DdPP1c) e mutante (DdPP1cF269C) de D. discoideum e NcPP1c de N. crassa in vitro. A proteína DdPP1cF269C apresenta características distintas da DdPP1c incluindo maior estabilidade, maior atividade de fosforilase fosfatase e maior sensibilidade frente ao inibidor caliculina A. Estas diferenças devem-se a substituição da cisteína conservada da posição 269 por uma fenilalanina, que é verificada na enzima selvagem. DdPP1c e DdPP1cF269C foram também ensaiadas na presença de INc-1L e INc-1 que são ortólogos de I-2 em N. crassa. Ambas as proteínas recombinantes purificadas exibiram efeito inibidor sobre a atividade de fosforilase fosfatase das DdPP1c recombinantes selvagem e mutante, sendo que INc-1 foi um inibidor duas vezes mais eficiente que INc-1L. Este efeito pode ser devido a um segmento de 38 aminoácidos codificado por um íntron em fase que é retido na isoforma INc-1L. Nossos dados indicam ainda que a mutação F269C não afetou a sensibilidade da DdPP1c recombinante a nenhum dos ortólogos de I-2 testados in vitro. Ensaios de duplo-híbrido utilizando a PP1c selvagem e mutante de D. discoideum (DdPP1c e DdPP1cF269C) e de N. crassa (NcPP1c) como iscas e DdI-2 como presa mostraram que estas proteínas interagiram in vivo. Quando a presa era o INc-1L ou INc-1 a interação ocorreu apenas com a NcPP1c, sendo mais forte no caso de INc-1. As regiões de DdI-2 envolvidas na interação física com a DdPP1c foram mapeadas através da expressão de proteínas truncadas no ensaio de duplo híbrido. Os experimentos apontaram que o carbóxi-terminal de ~100 aminoácidos não é essencial para a interação, mas que o somatório das diversas regiões responde pela integridade da interação. / The serine/threonine phosphatase of type-1 (PP1) is a ubiquous enzyme in the cells and tissues from several species studied and regulates numerous processes such as intermediate metabolism, mRNA splicing, transcription, and apoptosis. PP1 holoenzymes consist of a well-conserved catalytic subunit (PP1c) and one or more variable regulatory subunits. In mammals, more than fifty polypeptides that bind PP1c have been identified, originating holoenzymes with distinct cell locations and specificities. These proteins include cytosolic PP1c inhibitors such as inhibitor-1 (I-1), inhibitor-2 (I-2) and nuclear inhibitor of PP1 (NIPP-1). I-2 orthologs have also been described in Saccharomyces cerevisiae and Neurospora crassa. In the present work, we demonstrate that the genome of the social amoeba Dictyostelium discoideum has a single gene encoding for an I-2 ortholog (DdI-2). Northern blot analyses have shown that DdI-2 mRNA is expressed throughout Dictyostelium developmental cycle at variable levels. We also demonstrated that DdI-2 is a true PP1c inhibitor as its recombinant product is capable of inhibiting the phosphorylase phosphatase activity of wild-type PP1c (DdPP1c) and mutant (DdPP1cF269C) of D. discoideum and NcPP1c of N. crassa in vitro. DdPP1cF269C protein presents distint traits including higher stability, phosphorylase phosphatase activity and sensibility to calyculin A than the wild-type. These differences are originated from the replacement of a well conserved cisteine residue by a phenylalanine found in the wild-type. The wild-type and mutant DdPP1c have also been assayed in the presence of INc-1L and INc-1 which are orthologues to I-2 in N. crassa. Both purified recombinant proteins have shown inhibitory effects over phosphorylase phosphatase activities, with INc-1 being twice more potent than INc-1L. This might be due to the presence of an intron retention event in the latter that results in a insertion of 38 aminoacids. Our data also indicate that F269C mutation did not affect DdPP1c sensitivity to inhibition by all the three recombinant I-2 orthologues in vitro. Yeast two-hybrid assays using wild type (DdPP1c) and mutant (DdPP1cF269C) D. discoideum and N. crassa (NcPP1c) PP1c as preys and the putative inhibitor DdI-2 as a bait showed inequivocally that these proteins interacted in vivo. When the prey was INc-1 or INc-1L the interaction occured only with NcPP1c and was stronger with INc-1. The domains of DdI-2 involved in the interaction with DdPP1c were mapped by two-hybrid interaction assays with DdI-2 deleted mutants. These experiments have pointed out that the DdI-2 carboxi-terminus of ~100 aminoacids is not essential for the interaction but that the sum of all regions is responsible for the integrity of the interaction.

O objetivo deste trabalho é apresentar uma proposta para o ensino de colocações adverbiais em inglês, típicas da área de negócios, à luz da Linguística de Corpus. Para isso, compilamos um corpus monolíngue em inglês britânico e americano, composto de periódicos de negócios e relatórios de empresas disponíveis on-line, num total de 2.310.143 palavras. Com a ajuda do Wordsmith Tools 5.0 e de cálculos estatísticos (escores T e Informação Mútua), levantamos as vinte e cinco colocações adverbiais mais recorrentes no corpus de estudo e as analisamos quanto aos padrões léxicogramaticais. Feita a análise, selecionamos dentre essas unidades colocacionais aquelas que poderiam ser abordadas em sala de aula, segundo os critérios estabelecidos pela pesquisadora, com base na abordagem DDL de Tim Johns (1991), na modelagem de Carter (1998) e nas críticas feitas ao uso das linhas de concordância no ensino de línguas. Elaboramos, assim, exercícios que foram aplicados em três estudos-piloto, realizados com alunos de inglês para negócios de um instituto de idiomas privado, nos seguintes níveis: pré-intermediário, intermediário e intermediário superior. Nos três estudos, comprovamos a aplicabilidade dos exercícios e a importância da Linguística de Corpus como abordagem ao ensino de línguas. / The aim of this work is to put forward a proposal for teaching typical adverbial collocations in Business English, based on Corpus Linguistics principles. To accomplish that, we compiled a monolingual corpus in British and American English, composed of business press texts as well as company reports available on-line, totalling 2,310,429 words. With the use of Wordsmith Tools 5.0 and statistical scores (T-score and Mutual Information), we found the twenty-five most frequent adverbial collocations, which were lexically and grammatically analysed concerning their patterns. Next, from these collocational units we selected the ones which could be approached in class, following the criteria set by the researcher, based on Tim Johns DDL (JOHNS, 1991), Carters modelling (CARTER, 1998) and the objections raised concerning the use of concordance lines for language teaching. Thus, we planned exercises to be applied in three pilot studies, carried out with Business English students at a private language school in São Paulo, Brazil, at the following levels: preintermediate, intermediate and upper-intermediate. The three studies demonstrated that such exercises can be successfully used in Business English classes thus confirming the relevance of Corpus Linguistics as an approach to language teaching.

O objetivo deste trabalho é apresentar uma proposta para o ensino de colocações adverbiais em inglês, típicas da área de negócios, à luz da Linguística de Corpus. Para isso, compilamos um corpus monolíngue em inglês britânico e americano, composto de periódicos de negócios e relatórios de empresas disponíveis on-line, num total de 2.310.143 palavras. Com a ajuda do Wordsmith Tools 5.0 e de cálculos estatísticos (escores T e Informação Mútua), levantamos as vinte e cinco colocações adverbiais mais recorrentes no corpus de estudo e as analisamos quanto aos padrões léxicogramaticais. Feita a análise, selecionamos dentre essas unidades colocacionais aquelas que poderiam ser abordadas em sala de aula, segundo os critérios estabelecidos pela pesquisadora, com base na abordagem DDL de Tim Johns (1991), na modelagem de Carter (1998) e nas críticas feitas ao uso das linhas de concordância no ensino de línguas. Elaboramos, assim, exercícios que foram aplicados em três estudos-piloto, realizados com alunos de inglês para negócios de um instituto de idiomas privado, nos seguintes níveis: pré-intermediário, intermediário e intermediário superior. Nos três estudos, comprovamos a aplicabilidade dos exercícios e a importância da Linguística de Corpus como abordagem ao ensino de línguas. / The aim of this work is to put forward a proposal for teaching typical adverbial collocations in Business English, based on Corpus Linguistics principles. To accomplish that, we compiled a monolingual corpus in British and American English, composed of business press texts as well as company reports available on-line, totalling 2,310,429 words. With the use of Wordsmith Tools 5.0 and statistical scores (T-score and Mutual Information), we found the twenty-five most frequent adverbial collocations, which were lexically and grammatically analysed concerning their patterns. Next, from these collocational units we selected the ones which could be approached in class, following the criteria set by the researcher, based on Tim Johns DDL (JOHNS, 1991), Carters modelling (CARTER, 1998) and the objections raised concerning the use of concordance lines for language teaching. Thus, we planned exercises to be applied in three pilot studies, carried out with Business English students at a private language school in São Paulo, Brazil, at the following levels: preintermediate, intermediate and upper-intermediate. The three studies demonstrated that such exercises can be successfully used in Business English classes thus confirming the relevance of Corpus Linguistics as an approach to language teaching.

Syftet med denna studie var att utveckla en generell applikation som kan generera DDL-skript från tre olika databaser: Oracle, SQL Server och DB2, genom att enbart använda en JDBC-uppkoppling. Behovet av denna studie kommer från att databasadministratörer och utvecklare effektivt ska kunna hantera databaser med olika system med varierande syntax och struktur. Processen genomfördes i IDEAn IntelliJ med java.sql-APIt för databasoperationer. Resultatet visade att trots skillnaderna mellan dessa databaser var det möjligt att utveckla en generell process för att extrahera DDL-kod med endast en JDBCuppkoppling. Dock krävdes vissa specifika anpassningar för varje databassystem. En observation var hanteringen av primärnycklar och index mellan systemen. Denna applikation har potential att vidareutvecklas till ett kraftfullt verktyg för databashantering, vilket sparar tid och resurser. Områden för framtida undersökning inkluderar hantering av komplexa datatyper och strukturer, samt prestanda med stora databaser. / This study was aimed at developing a generic application capable of generating DDL-code from three different databases: Oracle, SQL Server and DB2 by using JDBC. This research necessity origins from database administrators and developers need to effectively manage databases across different systems with different syntax and structure. The process was conducted in the IDEA IntelliJ using the java.sql-API for database operations. The result showed that despite the differences between these databases it was possible to develop a generic process for extracting DDL-code only using a JDBC connection. However, some specific adaptions were required for each database system. An observation was the managing of primary keys and indexes across the systems. This application has the potential to be developed further into a powerful tool for database management that saves time and resources. Areas for further investigation is handling of complex data types and structures and performance with large databases.

Le flottement des tabliers de ponts flexibles constitue l'une des instabilités aérodynamiques les plus sévères pouvant survenir lors de tempêtes de vent. Le flottement est un phénomène dit auto-excité et se caractérise par de grandes amplitudes de déplacements dynamiques qui croissent avec la vitesse de vent et qui peuvent mener à la ruine de l'ouvrage. Avec l'évolution des matériaux, des techniques de constructions et des connaissances dans le domaine du génie civil, l'augmentation de la longueur des portées des ponts a pour effet de rendre ces structures plus flexibles. Historiquement, la participation du déplacement horizontal au flottement a été considérée comme négligeable. Toutefois, l'augmentation de la flexibilité latérale du tablier jumelée au couplage des modes dû aux caractéristiques des sections de tabliers et à l'interaction avec les câbles soulève quelques doutes concernant cette hypothèse. Le modèle mathématique actuellement utilisé pour représenter les forces auto-excitées de flottement a été développé par Scanlan et Tomko [1971]. Ce modèle définit des matrices de rigidité aérodynamique et d'amortissement aérodynamique qui varient en fonction de la vitesse réduite de l'écoulement. Ces deux matrices s'ajoutent aux matrices structurales et constituent la contribution aéroélastique de l'écoulement. Quelques recherches ont mis en évidence l'aspect non linéaire du flottement en démontrant la dépendance des coefficients instationnaires à la fréquence et à l'amplitude auxquelles ils ont été extraits. Les objectifs principaux de ce projet de recherche sont d'étudier l'effet du déplacement horizontal sur le flottement, d'étudier la dépendance des coefficients instationnaires à la fréquence et à l'amplitude d'extraction et d'élaborer une méthode expérimentale et un modèle mathématique permettant l'intégration temporelle non linéaire des forces auto-excitées. Des essais en régime libre sur 6 modèles sectionnels réguliers ont d'abord été réalisés pour évaluer l'effet du déplacement horizontal sur le flottement. Par la suite, une étude paramétrique de l'effet de la fréquence et de l'amplitude auxquelles les coefficients intationnaires sont extraits a été réalisée en régime forcé harmonique. Cette étude ayant mis en évidence la dépendance de certains coefficients à ces paramètres d'extraction, une dernière étude expérimentale a été réalisée. Cette dernière a permis l'extraction de coefficients de force dynamiques à partir de mouvements cycliques à vitesses ou accélérations absolues constantes. L'intégration de ces coefficients temporels, par opposition à fréquentiels, dans un outil de calcul dynamique transitoire non linéaire a permis la mise en évidence du flottement de sections de pont atteignant des amplitudes de vibration variant en fonction de la vitesse de l'écoulement. La vitesse de flottement obtenue concorde bien avec la moyenne des prédictions calculées à l'aide de la méthode classique. Un tel modèle permettrait à moyen terme de prendre en compte l'aspect non linéaire du flottement dans un modèle dynamique transitoire complet d'un pont, y incluant également les non-linéarités de nature structurale.

Main topic of this thesis is Win32 application programming interface and symbolic instructions programming language. It can be divided into four parts. First part sums the basics of 32-bit programming in symbolic instructions language in Windows. Second part describes the Win32 resources for computer intercommunication. Third part presents, describes and comments selected problems in symbolic instructions language.Last part covers a creation of an example application. This application and its parts will be the content of computer exercises in VUT Brno FEEC subject Počítače a jejich periferie.