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Reprodução em Characidium schubarti (Teleostei: Characiformes): análise da reprodução em cativeiro e de genes relacionados à diferenciação sexual (dmrt1, cyp19a1a e amh) / Reproduction in Characidium schubarti (Teleostei: Characiformes): analysis of captive breeding and of genes related to sex differentiation (dmrt1, cyp19a1a and amhGomes, Carolina Pereira 11 February 2014 (has links)
Os peixes teleósteos representam cerca de metade de todas as espécies de vertebrados já descritas. Esta rica diversidade reflete-se nas diferentes formas de determinação sexual, sendo as principais desencadeadas por influência ambiental e por fatores genéticos. Os genes envolvidos neste mecanismo têm sido caracterizados em diversas espécies de peixes teleósteos, como é o caso dos genes dmrt1 e amh que têm importante papel na diferenciação em machos de teleósteos e o gene cyp19a1a, que possui relação com a diferenciação sexual de fêmeas. Têm sido cada vez mais corrente na literatura, estudos envolvendo genes da via de diferenciação sexual em espécies de teleósteos, porém escassas são as informações em relação à ordem Characiformes. Além da genética, a biologia reprodutiva de peixes teleósteos engloba ainda estudos relativos ao papel dos hormônios e do ambiente na reprodução, inclusive na reprodução em cativeiro. A presente dissertação tem como principal objetivo a análise dos genes acima citados na espécie Characidium schubarti (Characiformes) - que possui pouca informação sobre sua biologia reprodutiva na literatura-; caracterizando fragmentos dos genes dmrt1 e cyp19a1a e a similaridade destes com os mesmos já descritos em outras ordens de peixes teleósteos. Além disso, foi possível no desenvolver do projeto, obter informações pertinentes em relação ao papel do ambiente e de hormônios exógenos na reprodução da espécie. Espera-se que com o aumento de pesquisas envolvendo genes relacionados ao sexo, o objetivo de controlar o gênero sexual em peixes economicamente importantes possa ser alcançado com sucesso a partir do controle da expressão de fatores da via de diferenciação / Teleost fishes represent about half of all vertebrate species already described. Such a rich diversity is reflected in different forms of sex determination, the main ones being triggered by environmental influences and genetic factors. Genes such as amh and dmrt1 are involved in this mechanism and have been characterized in several teleost fish species, they play an important role in the differentiation of teleost males whereas the cyp19a1a gene is related to the female sexual differentiation. Have been increasingly common in the literature, studies concerning the rule of genes in sex differentiation in teleosts, however pieces of information regarding the order Characiformes are scarce. Besides genetics, reproductive biology of teleost fishes also includes studies on the role of hormones and environment on reproduction, and also captive breeding. This thesis aims to analyze the aforementioned species in Characidium schubarti (Characiformes) genes for which little information exists on their reproductive biology literature featuring fragments of genes dmrt1 and cyp19a1a and the similarity of these with those already described in other orders of teleost fish . Moreover, it was possible to develop the project, obtaining relevant information regarding the role of the environment and exogenous hormones in reproduction of the species. It is expected that with the increase of research involving genes related to sex, in order to control the sexual gender for such an economically important fish can be successfully achieved from the control of the expression of factors of differentiation
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Reprodução em Characidium schubarti (Teleostei: Characiformes): análise da reprodução em cativeiro e de genes relacionados à diferenciação sexual (dmrt1, cyp19a1a e amh) / Reproduction in Characidium schubarti (Teleostei: Characiformes): analysis of captive breeding and of genes related to sex differentiation (dmrt1, cyp19a1a and amhCarolina Pereira Gomes 11 February 2014 (has links)
Os peixes teleósteos representam cerca de metade de todas as espécies de vertebrados já descritas. Esta rica diversidade reflete-se nas diferentes formas de determinação sexual, sendo as principais desencadeadas por influência ambiental e por fatores genéticos. Os genes envolvidos neste mecanismo têm sido caracterizados em diversas espécies de peixes teleósteos, como é o caso dos genes dmrt1 e amh que têm importante papel na diferenciação em machos de teleósteos e o gene cyp19a1a, que possui relação com a diferenciação sexual de fêmeas. Têm sido cada vez mais corrente na literatura, estudos envolvendo genes da via de diferenciação sexual em espécies de teleósteos, porém escassas são as informações em relação à ordem Characiformes. Além da genética, a biologia reprodutiva de peixes teleósteos engloba ainda estudos relativos ao papel dos hormônios e do ambiente na reprodução, inclusive na reprodução em cativeiro. A presente dissertação tem como principal objetivo a análise dos genes acima citados na espécie Characidium schubarti (Characiformes) - que possui pouca informação sobre sua biologia reprodutiva na literatura-; caracterizando fragmentos dos genes dmrt1 e cyp19a1a e a similaridade destes com os mesmos já descritos em outras ordens de peixes teleósteos. Além disso, foi possível no desenvolver do projeto, obter informações pertinentes em relação ao papel do ambiente e de hormônios exógenos na reprodução da espécie. Espera-se que com o aumento de pesquisas envolvendo genes relacionados ao sexo, o objetivo de controlar o gênero sexual em peixes economicamente importantes possa ser alcançado com sucesso a partir do controle da expressão de fatores da via de diferenciação / Teleost fishes represent about half of all vertebrate species already described. Such a rich diversity is reflected in different forms of sex determination, the main ones being triggered by environmental influences and genetic factors. Genes such as amh and dmrt1 are involved in this mechanism and have been characterized in several teleost fish species, they play an important role in the differentiation of teleost males whereas the cyp19a1a gene is related to the female sexual differentiation. Have been increasingly common in the literature, studies concerning the rule of genes in sex differentiation in teleosts, however pieces of information regarding the order Characiformes are scarce. Besides genetics, reproductive biology of teleost fishes also includes studies on the role of hormones and environment on reproduction, and also captive breeding. This thesis aims to analyze the aforementioned species in Characidium schubarti (Characiformes) genes for which little information exists on their reproductive biology literature featuring fragments of genes dmrt1 and cyp19a1a and the similarity of these with those already described in other orders of teleost fish . Moreover, it was possible to develop the project, obtaining relevant information regarding the role of the environment and exogenous hormones in reproduction of the species. It is expected that with the increase of research involving genes related to sex, in order to control the sexual gender for such an economically important fish can be successfully achieved from the control of the expression of factors of differentiation
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Sexual Plasticity in a Marine Goby (Lythrypnus dalli): Social, Endocrine, and Genetic Influences on Functional SexRodgers, Edmund William 03 December 2007 (has links)
Sex determination occurs early in development for most animals, at which time sex is fixed for life. Many teleost fishes, however, exhibit remarkable sexual plasticity throughout their life history, ranging from multiple morphs within a sex to functional adult sex reversal. To understand the development and evolution of adult sex reversal, I examined behavioral, endocrine, and genetic contributions to the regulation of functional sex in adult animals, using the bluebanded goby (Lythrypnus dalli) as an experimental model. This species was found to be equally capable of sexual transitions from female to male (protogyny) as from male to female (protandry). Throughout adult life, sexual phenotype is determined by social status, an emergent property of agonistic behavioral interactions that follows a relatively simple social convention: if dominant become or remain male, or if subordinate, become or remain female. The translation of social status into a change in sexual phenotype in the protogynous direction requires a rapid drop in circulating estrogens and an increase in the gonadal expression of a testis differentiating gene dmrt1. Steroid hormones do not play a significant role in modulating status, but the androgen 11-ketotestosterone does positively correlate with the expression of paternal behavior. Taken together, these findings suggest an evolutionary mechanism in sexually plastic species that has linked the conserved molecular cascades of sexual differentiation to a novel signal that varies over life history, social status, thereby allowing for lifelong phenotypic plasticity.
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Expression profiling and function analyses on avian sex-determining candidate genes, DMRT1 and HINT1Tsai, Hsin-yin 15 July 2004 (has links)
To establish the gene expression profile and cascade subsequently on avian sex-determining candidate genes, seven avian sex-determining candidate genes including DMRT1, FET1, FOXL2, LHX9, HINT1, SMC2L1 and SOX9 were analyzed at early embryogenesis. Quantitative reverse transcription PCR (Quantitative RT-PCR) technology was used to establish the gene expression profiles among these genes at four, five, six and seven days of embryos. The results of quantitative RT-PCR reveal that the DMRT1 was expressed in chicken embryos of both sexes. DMRT1 gene expressions were up-regulated at four, five and six days of chicken embryos. DMRT1 expression increased at 5-Dpc. of male embryos, however, expression was not signification different in females embryos. Gene expression of FET1, FOXL2, LHX9 and HINT1 were higher in females than in males. The SMC2L1 and SOX9 were expressed in both sexes. Also, to identify the novel sex-determination genes in early chicken subtractive embryos, cDNA libraries from male-minus-female and female-minus-male 3.5 Dpc. embryos cDNA were established. Gene annotation was carried out by data-mining in public databases, GeneBank (NCBI, USA) and TIGR gene indices (The Institute for Genome Research, USA). A total 548 of colonies in male-minus-female library and 79 sequences were annotated. However, a total of 589 of colonies in female-minus-male library and 16 sequences were annotated. Sequences were homologous to the steroid 5£\-reductase protein (SRD5A1) using BLASTx in male-minus-female subtractive library. The SRD5A1 may play a sex-differentiation role in male chicken. We need more study to know function of steroid 5£\-reductase protein in future.
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In Silico and Molecular Cloning of Muscovy Sex-determining Candidate Gene DMRT1Wang, Yi-Teen 25 July 2002 (has links)
To produce male Muscovy only for fatty liver and meat-type production is an important economic goal in animal husbandry, although the sex-determining mechanism in poultry remains to be elucidated. Manipulation of sex-determining gene(s) in poultry provides enormous opportunities on the development of sex pre-selection reproductive systems. DSX and MAB-3 genes in Drosophila and C. elegans are conserved across the human, mice, chickens, fish, turtles, and reptiles revealing an ancient sex-determining locus DMRT1. Thus the Z-linked, DMRT1 in chicken is an excellent candidate regulatory gene controlling similar aspects of sexual development in poultry. This dissertation is aimed to clone and characterize Muscovy DMRT1 gene for further application in sex pre-selection. Partial cDNA sequences of Muscovy DMRT1 was determined and revealed 95% identity and 83% with chicken and red-eared slider turtle DMRT1 cDNA sequences. DMRT1 orthologs among various species were analyzed by Phylip program and phylogenetic tree was constructed by MEGA2 programs. Results indicated that Muscovy, chicken and red-eared slider turtle DMRT1 revealing 95%, and 83% identity at cDNA and 61%, 54% identity at amino acid level.
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Cibles et voies de signalisation régulées par FOXL2 au cours de la morphogenèse ovarienne / Target Genes and Signaling Pathways Regulated by FOXL2 During Ovarian DifferentiationEl Zaiat, Maëva 16 October 2015 (has links)
FOXL2 est un facteur de transcription crucial pour la fonction ovarienne. Dans l'espèce humaine, des mutations hétérozygotes de ce gène sont responsables de la survenue d'un syndrome associant des malformations des paupières à une insuffisance ovarienne prématurée. De même chez la souris, l'invalidation totale de Foxl2 conduit à un blocage de la folliculogenèse et donc à une infertilité femelle. Chez la chèvre, la mutation Polled Intersex Syndrome (PIS) engendre le silence transcriptionnel de FOXL2 dans les gonades XX PIS-/- ce qui conduit à une inversion sexuelle et à la différenciation de testicules à la place d'ovaires chez les animaux génétiquement femelles homozygotes pour la mutation (inversion sexuelle de type mâle XX). FOXL2 est donc déterminant pour la différenciation ovarienne très précocement au cours du développement dans l'espèce caprine, alors qu'il ne semble impliqué que plus tardivement dans l'établissement de la fertilité chez la souris et la femme. Afin de comprendre ces différences entre espèces, nous avons recherché quels étaient les gènes et les voies de signalisation régulés par FOXL2 dans l'ovaire de chèvre au début de sa différenciation. Grâce à un séquençage à haut-débit des transcrits présents dans trois types de gonades caprines (testicules XY, ovaires XX et gonades XX PIS-/- (qui n'expriment pas FOXL2)) au début de leur différenciation, nous avons pu (i) mieux caractériser le rôle de FOXL2 dans l'ovaire caprin et montrer qu'il y agit avant tout comme un facteur anti-testiculaire, et (ii) mettre en évidence de nouveaux gènes pro-ovariens comme DMXL2 et étudier son rôle putatif dans la fonction ovarienne grâce à des expériences fonctionnelles chez la souris. / FOXL2 is a transcription factor which is crucial for the ovary. In humans, heterozygous mutations are responsible for the BPES syndrome characterized by eyelid anomalies and premature ovarian failure. Similarly in mice, Foxl2 invalidation leads to complete folliculogenesis disruption and female infertility. In the goat, the Polled Intersex Syndrome mutation is responsible for the transcriptional silencing of FOXL2 in XX PIS-/- gonads that leads to female-to-male sex reversal and the differentiation of testes instead of ovaries in genetically female animals homozygous for the mutation. Thus, FOXL2 is determining for ovarian differentiation early during development in goats, whereas it is involved in fertility tardily in mice and women. In order to understand these species-specific differences, we searched for the genes and pathways regulated by FOXL2 in early goat ovaries. Thanks to RNA-sequencing of goat XY testes, XX ovaries and XX PIS-/- gonads (lacking FOXL2) at the beginning of their differentiation, we were able to (i) better characterize the role of FOXL2 in goat ovaries and show that it acts mainly as an anti-testis factor, and (ii) highlight new pro-ovarian genes like DMXL2, and study its putative role during ovarian development using functional experiments in the mouse.
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Elucidating the molecular network underlying temperature-dependent sex determination in the red-eared slider turtle, Trachemys scriptaShoemaker, Christina May 13 August 2012 (has links)
Components of the molecular pathway underlying gonadogenesis in organisms with temperature-dependent sex determination (TSD) have been retained from genetic sex determination. Furthermore, although much of this network has been conserved, new functions for these genes have evolved in this different mode of sex determination. We find that the transcription factors Sox9 and Dmrt1 and the hormone Mis are involved in the formation of a testis and/or the repression of an ovary at a male-producing temperature. While Mis expression may be maintained by Sox9, the initial upregulation of Mis in the developing testis is most likely modulated by some other upstream factor. Dmrt1 appears to play an upstream role in testis sex determination. We provide evidence that the transcription factor Dax1 and the signaling molecule Wnt4, cloned for the first time in an organism with TSD, play roles in gonadogenesis in both sexes. Finally, we show that the transcription factor FoxL2 and the signaling molecule Rspo1 are involved in the formation of an ovary and/or the repression of a testis at a female-producing temperature. In the first investigation of Rspo1 in any organism exhibiting TSD, we demonstrate it is involved upstream in ovarian sex determination. Complementary to descriptive studies, we optimize a whole organ culture system in which gonad explants develop in vitro for up to three weeks. We show that expression of the sex-determining network in isolated gonads mimics in ovo patterns, revealing an endogenous temperature-sensing mechanism that does not require other embryonic tissues. Ectopic expression of Sox9 reveals a possible positive feedback regulation of Dmrt1. The use of this culture system opens the door to functional manipulation of the gonad at the molecular level and is suitable for a myriad of future studies. This work makes strides in elucidating the molecular network underlying gonadogenesis in an organism exhibiting TSD, and invites investigation of the evolution of gene function. The data lend insight into the changing roles of molecules in sex determination across diverse taxa, and into the evolution of developmental pathways in general. / text
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Functional Analysis of the Sex Related Gene dmrt1 in Xenopus / Mechanistic investigation of the sex related gene dmrt1 in African clawed frogs (Xenopus) evidences both neofunctionalization and subfunctionalizationKukoly, Lindsey 11 1900 (has links)
Sex determination is a key developmental process in several species regulated by sexrelated
transcription factors. In many species a gene called doublesex and mab-3 related
transcription factor 1 (dmrt1), plays an important role in sexual differentiation. I used African
clawed frogs (Xenopus) to examine function of dmrt1 in two species: a diploid species, X.
tropicalis, and an allotetraploid species, X. laevis. In both species, dmrt1 is an autosomal gene;
Xenopus tropicalis has one copy of dmrt1 and X. laevis has two homeologous copies that by
definition are derived from whole genome duplication: dmrt1.L and dmrt1.S in X. laevis. We
generated knockouts of each of these genes to further examine their function in sexual
differentiation. Histological examination showed testicular dysgenesis in X. tropicalis dmrt1 and
X. laevis dmrt1.L null males whereas dmrt1.S null males presented no obvious difference in
sperm density compared to wildtype males. X. tropicalis dmrt1 and X. laevis dmrt1.L null
females were found to completely lack reproductive organs and are infertile whereas dmrt1.S
null females appeared unaffected. The contrasting results between dmrt1.L and dmrt1.S in X.
laevis provides evidence of both neofunctionalization and subfunctionalization following gene
duplication and suggest that gene duplication is a major contributor to evolutionary change.
Additional investigation of the transcriptome of these frogs and the role of dmrt1 in the
secondary sex characteristic vocalization provides further evidence of the role of dmrt1 in
development. Comprehensively, this investigation provides further knowledge of the role of
dmrt1 and homeologs of this gene in sexual differentiation and introduces a novel aspect of this
gene in female development. Future efforts are focused on generating double knockouts for
dmrt1.L and dmrt1.S, further examining the role of dmrt1.S in somatic cell function and
developing additional mutant lines in other Xenopus for comparative analysis. / Thesis / Master of Science (MSc) / In many species sexual differentiation is a crucial developmental event. Surprisingly,
however, the systems orchestrating sexual differentiation are highly variable among species. The
doublesex and mab-3 related transcription factor 1 (dmrt1) gene plays a role in sexual
differentiation in many groups, but its specific roles in this process are incompletely
characterized and potentially diverse. We used genetic engineering in two species of African
clawed frog (Xenopus) to disable function of dmrt1 in order to explore effects on gonadal
development and the development of secondary sex characteristics. We found that dmrt1 is
required for normal ovary or testis development in both Xenopus species, and that functional
divergence occurred following duplication of dmrt1 by whole genome duplication. Taken
together, these findings identify previously uncharacterized roles of dmrt1 in Xenopus and
provide evidence of dynamic functional evolution of this important gene.
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Études comparatives, évolutives et recherche de gènes importants pour la détermination du sexe chez les mammifèresBoyer, Alexandre January 2005 (has links)
Thèse numérisée par la Direction des bibliothèques de l'Université de Montréal.
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Pesquisa de mutações no gene DMRT1 em pacientes portadores de distúrbios do desenvolvimento sexual (DDS) 46,XY por anormalidades gonadais / Search of mutation on DMRT1 gene in patients with 46,XY disorders of sex development (DSD) by gonads abnormalitiesSilva, Thatiana Evilen da 14 September 2012 (has links)
Introdução: O gene DMRT1 é um fator muito importante, o qual induz a determinação sexual masculina. Estudos mais recentes têm demonstrado que o Dmrt1 possui um papel significante no desenvolvimento ovariano. Deleções restritas ao gene DMRT1 têm sido raramente identificadas em pacientes com disgenesia gonadal (DG) sem outras características sindrômicas. Objetivo: Pesquisar a presença de haploinsuficiência do gene DMRT1 (deleções e/ou mutações inativadoras) em um grupo grande de pacientes não sindrômicos com distúrbios do desenvolvimento sexual (DDS) por anormalidades gonadais. Polimorfismos do DMRT1, como fatores potenciais pelas anormalidades gonadais, foram também identificados. Pacientes e Métodos: Foram avaliados cerca de 39 pacientes portadores de DDS por anormalidades do desenvolvimento gonadal 46,XY: 24 com disgenesia gonadal parcial e 15 pacientes com disgenesia gonadal completa. As regiões codificadoras do DMRT1 e o domínio DM (exon 1) foram amplificados e sequenciados. A análise de Multiplex ligation probe amplification (MLPA) do DMRT1 foi realizada usando um kit comercial. Resultados: Deleção parcial ou total do DMRT1 não foi identificada pela técnica de MLPA. Oito variantes alélicas do DMRT1 foram identificados. Uma nova variante c.968-15insTTCTCTCT foi identificada em 6,4% e em 14,3% dos alelos dos pacientes 46,XY e indivíduos controles, respectivamente. Conclusão: Este estudo sugere que deleções parciais ou completas no DMRT1 e mutações inativadoras não são frequentemente encontradas em pacientes com anormalidades do desenvolvimento gonadal. Além disso, nenhuma das variantes alélicas identificadas neste grupo de pacientes poderia ser considerada como um marcador potencial polimórfico para disgenesia gonadal / Introduction Dmrt1 gene is a very important factor in inducing male sex determination, and more recently it has been demonstrated that Dmrt1 plays a significant role in ovary development. DMRT1 deletions have rarely been identified in patients with 46,XY gonadal dysgenesis (GD) without syndromic features. Objective- To screen for the presence of DMRT1 haploinsufficiency (deletions and/or inactivating mutations) in a large cohort of non-syndromic patients with disorder of sex development (DSD) due to abnormalities of gonadal development. DMRT1 polymorphisms, as potential susceptibility factors for gonadal abnormalities, were also investigated. Subjects and Methods- We evaluated 39 patients with 46,XY GD: 24 patients with the partial, and 15 with the complete form. The entire coding region (éxons 2-5) of DMRT1 and the DM domain (exon 1) were PCR-amplified and direct sequenced. Multiplex ligation probe amplification (MLPA) analysis of DMRT1 was carried out using a commercial kit. Results- Partial or total deletion of DMRT1 was not identified by MLPA technique. Eight allelic variants of DMRT1 were identified. The novel variant c.968-15insTTCTCTCT was identified in 6.4% and in 14.3% of the alleles of 46,XY patients and control subjects, respectively Conclusion- This study suggest that complete or partial DMRT1 deletions and inactivating mutations are not frequently found in patients with abnormalities of gonadal development. Additionally, none of the allelic variants identified in this cohort of patients could be considered a potential polymorphic susceptibility marker for gonadal dysgenesis
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