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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
61

Studies on the mechanisms of coenzyme A biosynthesis in the Archaea / アーキアにおける coenzyme A 生合成機構に関する研究

Tomita, Hiroya 24 March 2014 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(工学) / 甲第18304号 / 工博第3896号 / 新制||工||1598(附属図書館) / 31162 / 京都大学大学院工学研究科合成・生物化学専攻 / (主査)教授 跡見 晴幸, 教授 森 泰生, 教授 濵地 格 / 学位規則第4条第1項該当 / Doctor of Philosophy (Engineering) / Kyoto University / DGAM
62

Purification and characterization of mammalian tyrosine decarboxylase activity

Bowsher, Ronald R. January 1981 (has links)
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
63

Molecular cloning of the bovine ornithine decarboxylase gene and the detection of trait-associated DNA polymorphisms in the bovine ornithine decarboxylase and growth hormone genes.

Yao, Jianbo. January 1997 (has links)
No description available.
64

Multiple routes of phosphatidylethanolamine biogenesis ensure membrane integrity of Toxoplasma gondii

Hartmann, Anne Kathrin 20 April 2016 (has links)
Toxoplasma gondii ist ein weit verbreiteter, obligat-intrazellulärer, einzelliger Parasit, der die lebensbedrohliche Krankheit Toxoplasmose in Menschen und Tieren hervorrufen kann. Der schnell replizierende Parasit benötigt erhebliche Mengen an Phospholipiden zur Biogenese intra- und extrazellulärer Membranen. Phosphatidylethanolamin (PtdEtn) ist ein wichtiges und ubiquitäres Phospholipid in Pro- und Eukaryoten und das zweithäufigste Lipid in T. gondii. Dieses kann de novo über den CDP-Ethanolamin Stoffwechselweg oder durch Decarboxylierung von Phosphatidylserin synthetisiert werden. Im Rahmen dieser Arbeit konnte die Expression von zwei distinkten Phosphatidylserin Decarboxylasen (PSDs) in T. gondii nachgewiesen werden: TgPSD1pv ist partiell löslich und wird über Dichte Granula in die Parasitophore Vakuole sekretiert, während sich TgPSD1mt im Mitochondrium von Tachyzoiten befindet. TgPSD1mt ist in der Lage einen Ethanolamin-auxotrophen S. cerevisiae Stamm zu komplementieren. Ein Knock-down von TgPSD1mt in T. gondii verursacht eine verlangsamte Parasitenreplikation, welche zu einem verminderten in vitro Wachstum führt. Der PtdEtn-Gehalt in der Mutante bleibt unverändert, was auf eine stringente Homöostase des zellulären PtdEtn Reservoirs durch alternative Lipidbiogenesewege hindeutet. Tatsächlich verfügt T. gondii zusätzlich über einen aktiven CDP-Ethanolamin Stoffwechselweg im Endoplasmatischen Retikulum, welcher den Verlust von TgPSD1mt partiell kompensieren kann. Das zweite, sekretierte TgPSD1pv-Enzym hingegen scheint für das Parasitenwachstum in vitro entbehrlich zu sein. Infektionsversuche mit radioaktiv markierten Wirtszellen zeigten zudem eine Aufnahme von PtdEtn oder PtdEtn-Derivaten in intrazellulär replizierenden Tachyzoiten. Diese Ergebnisse demonstrieren eine außergewöhnliche Kompartmentalisierung und Plastizität der PtdEtn-Synthese in T. gondii. / Toxoplasma gondii is a remarkably successful and widespread obligate intracellular protozoan parasite, which can cause the potentially life threatening disease Toxoplasmosis in humans and animals. The fast proliferating parasite requires a significant amount of phospholipids for biogenesis of organelles and enclosing vacuolar membranes. Phosphatidylethanolamine (PtdEtn) is one of the most ubiquitous phospholipids and the second most abundant lipid in T. gondii. It can be produced de novo by the CDP-ethanolamine pathway or by decarboxylation of phosphatidylserine. This work revealed the expression of two distinct PtdSer decarboxylase (PSD) enzymes in T. gondii: One of which is Coccidia-specific and partially soluble and secreted into the parasitophorous vacuole via dense granules (TgPSD1pv), and a second enzyme that localizes in the mitochondrion (TgPSD1mt) of tachyzoites. The mitochondrial PSD can complement a S. cerevisiae mutant auxotrophic for ethanolamine. A conditional knockdown of the TgPSD1mt gene impairs the parasite growth in vitro. Surprisingly, the mutant displayed an unaltered total PtdEtn content, which suggests a stringent homeostasis of the cellular PtdEtn pool by alternative routes of lipid biogenesis. Consistently, the parasite encodes an active CDP-ethanolamine pathway in the endoplasmic reticulum. Metabolic labeling of the TgPSD1mt mutant displayed an increased utilization of ethanolamine into PtdEtn, indicating an upregulation of the de novo CDP-ethanolamine pathway. Likewise, exogenous ethanolamine partially restored the growth phenotype of the mutant. In contrast, the TgPSD1pv enzyme is dispensable for the parasite growth. Host cell pre-labeling with radioactive ethanolamine indicated a potential uptake of host-derived PtdEtn or PtdEtn-derivates by intracellular parasites. Taken together, these results demonstrate an exceptional compartmentalization and plasticity of the PtdEtn synthesis in T. gondii.
65

Reaction mechanism of hOMPD and CaAAD at atomic resolution

Rindfleisch, Sören 07 February 2019 (has links)
No description available.
66

Metabolismo de poliaminas na embriogênese zigótica e somática de Araucaria angustifolia (Bertol.) Kuntze. / Polyamine metabolism in zygotic and somatic embryogenesis of Araucaria angustifolia (Bertol.) Kuntze

Oliveira, Leandro Francisco de 05 May 2017 (has links)
A Araucaria angustifolia é uma conífera nativa do Brasil. Em função da sua intensa exploração florestal, a espécie ocupa apenas 2% de sua vegetação natural. Neste sistema, a aplicação de técnicas biotecnológicas, como a embriogênese somática, podem ser integradas a programas de melhoramento genético e conservação. A similaridade entre a embriogênese somática e zigótica, tem sido utilizada para o estabelecimento de estudos visando o aperfeiçoamento do cultivo in vitro dos embriões somáticos, bem como para um maior conhecimento dos aspectos moleculares e fisiológicos que regulam a embriogênese. O metabolismo de poliaminas (PAs), mais especificamente putrescina, espermidina e espermina, tem se mostrado como fundamental para a compreensão e evolução da embriogênese zigótica e somática. Entretanto, a biossíntese das PAs e seu envolvimento nos vários processos biológicos que regulam a embriogênese, são pouco conhecidas em coníferas. Inserido nessa perspectiva, o presente trabalho teve como objetivo o estudo do metabolismo de PAs durante três estádios de desenvolvimento da semente (contendo as fases da embriogênese inicial até a tardia) e na proliferação de linhagens embriogênicas com diferentes potenciais embriogênicos de A. angustifolia. Foram investigados: a) os perfis de PAs (livres e conjugadas) e aminoácidos; b) determinação da via preferencial da biossíntese de putrescina, através da atividade enzimática da arginina descarboxilase (ADC) e ornitina descarboxilase (ODC); c) identificação e caracterização do padrão de expressão dos genes envolvidos no metabolismo de PAs; e d) a identificação das relações entre os perfis de PAs e aminoácidos presentes nas sementes das matrizes, e sua potencial influência nas fases de indução, proliferação e maturação dos embriões somáticos. Durante a embriogênese zigótica, a expressão dos genes AaADC (arginina descarboxilase) e AaSAMDC (S-adenosilmetionina descarboxilase) aumentaram no estádio cotiledonar, juntamente com o aumento de PAs. A biossíntese da putrescina é realizada preferencialmente via ADC, enquanto que a citrulina foi o principal aminoácido presente nas sementes. Em relação ao metabolismo de PAs nas culturas embriogênicas, os dados obtidos demonstraram que a arginina e ornitina parecem ter diferentes funções em cada linhagem testada. Na linhagem com alto potencial embriogênico, a arginina parece estar associada com a ativação dos genes relacionados ao catabolismo de PAs (AaPAO2, AaCuAO e AaALDH), enquanto que esse efeito não foi observado na linhagem bloqueada. A ODC tem uma maior atividade na linhagem responsiva, enquanto que na linhagem bloqueada, as atividades da ADC e ODC são similares. Dependendo da matriz foram observados diferentes perfis de PAs e aminoácidos, sendo estes perfis relacionados com as taxas de indução, proliferação e desenvolvimento dos embriões somáticos. Putrescina total, ornitina e asparagina foram os metabólitos diferencialmente identificados entre as matrizes, os quais podem ser propostos como marcadores bioquímicos para a seleção de matrizes com alto potencial para a embriogênese somática. Os resultados obtidos fornecem informações relevantes e inéditas sobre o metabolismo de PAs e aminoácidos na embriogênese zigótica e somática de A. angustifolia, bem como fornece novos subsídios para o aprimoramento das condições artificiais utilizadas para o desenvolvimento dos embriões somáticos / The Araucaria angustifolia is a native conifer species of Brazil. Due to its intense exploitation, the species cover only 2% of its original forest area. In this system, biotechnological tools, like somatic embryogenesis, may be integrated into breeding and conservation programs. The similarity between zygotic and somatic embryogenesis have been used to establishment of studies in order to optimization of somatic embryos in vitro culture, as well as for a better understanding of physiologic and molecular aspects that modulates the embryogenesis. The metabolism of polyamines (PAs), specifically putrescine, spermidine and spermine, has been demonstrated as fundamental for the comprehension and evolution of zygotic and somatic embryogenesis. However, the biosynthetic pathways of PAs and their involvement in various biological process that regulate the embryogenesis are little known in conifers. Inserted in this perspective, the aim of the current work was to study the metabolism of PAs during three seeds development stages (containing the early till late embryogenesis phases) and in proliferation of cell lines with different embryogenic potential of A. angustifolia. Were investigated: a) PAs (free and conjugated) and amino acids profiles; b) determination of preferential pathway for putrescine biosynthesis, through enzymatic activity of arginine decarboxylase (ADC) and ornithine decarboxylase (ODC); c) identification and characterization of gene expression profile of genes related to metabolism of PAs; and d) identification of the relationship between PAs and amino acids profiles in seeds of mother plants, and their potential influence in initiation, proliferation and maturation phases of somatic embryos. During the zygotic embryogenesis, AaADC (arginine decarboxylase) and AaSAMDC (S-adenosylmethionine decarboxylase) genes were up-regulated at cotyledonary stage along with the increasing of PAs. The biosynthesis of putrescine is performed preferentially by ADC pathway, while citrulline was the main amino acid recorded during the seed development. Regarding the metabolism of PAs in embryogenic cultures, the data demonstrated that arginine and ornithine seem to have different functions in each cell line tested. In cell line with high embryogenic potential, arginine seems to be associated to activation of genes related to PAs catabolism (AaPAO2, AaCuAO e AaALDH), while in blocked cell line this effect was not observed. ODC has a higher enzymatic activity in responsive cell line, while in blocked cell line, both ADC and ODC activities are similar. Depending of mother plant, were observed different PAs and amino acids profiles, being these profiles related with the rate of initiation, proliferation and maturation of somatic embryos. Total putrescine, ornithine and asparagine were the differentially metabolites identified between the mother plants, which can be proposed as biochemical marker to select mother plant with high potential to somatic embryogenesis. The results obtained provide relevant and inedited information about the metabolism of PAs and amino acids in zygotic and somatic embryogenesis of A. angustifolia, as well as provide news subsidies for optimization of in vitro conditions for somatic embryos development
67

Phytomonas serpens: caracterização da piruvato/indolpiruvato descarboxilase e funcionalidade da auxina produzida. / Phytomonas serpens: characterization of the pyruvate/indolepyruvate decarboxylase and functionality of the auxin produced.

Vançan, Susan Ienne da Silva 22 May 2012 (has links)
Um gene que codifica uma piruvato/indolpiruvato descarboxilase (PDC/IPDC) está presente no tripanossomatídeo de plantas Phytomonas serpens. A PDC atua na fermentação alcoólica, enquanto que a IPDC atua na biossíntese do fitormônio ácido indol-3-acético (AIA). Análises filogenéticas indicam que a PDC/IPDC de P. serpens é monofilética com IPDCs de gama-proteobactérias, sugerindo um evento de transferência horizontal gênica. A análise de meios de cultura de P. serpens confirma a produção de etanol e AIA. A funcionalidade do fitormônio foi confirmada em ensaios de alongamento de hipocótilos de tomateiros. Tomates inoculados com P. serpens mostraram aumento no teor de AIA-amida e -éster conjugados. A atividade PDC foi mostrada em extratos de P. serpens. Concluímos que a PDC/IPDC seria uma 2-cetoácido descaboxilase com atividade catalítica variável para diferentes substratos. A atividade PDC parece ser predominante em P. serpens, representando um mecanismo para oxidar parte do NADH formado na glicólise, principal responsável pela produção de ATP neste organismo. / A gene codifying a pyruvate/indolepyruvate decarboxylase (PDC/IPDC) is present in the plant trypanosomatid Phytomonas serpens. PDC acts in the alcoholic fermentation, whyle IPDC acts in the biosynthesis of the phytohormone indole-3-acetic acid (IAA). Phylogenetic analysis indicate that P. serpens PDC/IPDC is monophyletic with gamma-proteobacteria IPDCs, suggesting a horizontal gene transfer event. Analysis of P. serpens culture media confirms production of ethanol and IAA. The functionality of the phytohormone was confirmed by tomato hypocotyl elongation tests. Tomatoes inoculated with P. serpens showed an increase in the concentration of IAA amide and ester conjugated. PDC activity was shown in P. serpens extracts. We conclude that the PDC/IPDC would be a 2-keto acid decaboxylase with variable catalytic activity for different substrates. The PDC activity appears to be prevalent in P. serpens representing a mechanism to oxidize part of NADH formed in glycolysis, responsible for ATP production in this organism.
68

Structural and Functional Studies on Pyridoxal Kinase and Pyridoxal 5′-phosphate Dependent Enzymes

Deka, Geeta January 2017 (has links) (PDF)
Most of the chemical reactions of living cells are catalyzed by protein enzymes. These enzymes are very efficient and display a high degree of specificity with respect to the reaction catalyzed. Cellular activities depend critically on the precise three-dimensional structure and function of thousands of enzymes. Many enzymes require binding of metal ions or small organic molecules for their function. The organic molecules that are indispensible components of catalysis by proteins are called coenzymes. Pyridoxal 5ʹ-phosphate (PLP) is a versatile coenzyme found in all living cells. PLP-dependent enzymes play a key role in the function of most of the enzymes catalyzing reactions in the metabolic pathways of amino acid synthesis and degradation. The enzyme pyridoxal kinase serves to make available the co-enzyme PLP to apo-PLP dependent enzymes. Because of their key role in cellular function and their medical importance, the structure and function of PLP-dependent enzymes have been extensively investigated. In the past decade, detailed investigations on the structure and function of several PLP-dependent enzymes have been carried out in our laboratory. The enzymes studied are B. subtilis serinehydroxymethyl transferase (SHMT), S. typhimurium acetylornithine aminotransferase (AcOAT), S. typhimurium and E. coli diaminopropionate ammonia lyase (DAPAL), S. typhimurium D-serine dehydratase (DSD), S. typhimurium D-cysteine desulfhydrase (DCyD) and S. typhimurium arginine decarboxylase (ArgD). The extensive studies conducted on PLP-dependent enzymes in our laboratory during the past decade has not only resulted in deeper understanding of their structure and function but also raised several new questions regarding substrate recognition, reaction specificity, role of active site residues in the catalytic reaction, mechanism of catalysis and potential applications of these enzymes. This thesis is an attempt to answer some of these questions. The thesis also presents the structure and function of a new protein, Salmonella typhimurium pyridoxal kinase, the enzyme that provides PLP for PLP-dependent enzymes. Single crystal X-ray diffraction technique is the most powerful tool currently available for the elucidation of the three-dimensional structures of proteins and other biological macromolecules and for revealing the relationship between their structure and function. X-ray diffraction studies have provided in depth understanding of the topology of secondary structural elements in the three-dimensional structures of proteins, the hierarchical organization of protein domains, structural basis for the substrate specificity of enzymes, intricate details of mechanisms of enzyme catalyzed reactions, allosteric regulation of enzyme activity, mechanisms of feed-back inhibition, structural basis of protein stability, symmetry of oligomeric proteins and their possible biological implications and a myriad of other biochemical and biophysical properties of proteins. The work reported in this thesis is primarily based on X-ray diffraction studies. X-ray crystal structure investigations are complemented by spectral and biochemical studies on the catalyzed reactions. The thesis begins with an introduction to PLP-dependent enzymes and presentation of a brief summary of the earlier work carried out in our laboratory on PLP-dependent enzymes (Chapter 1). A brief description of earlier functional classification of PLP-dependent enzymes and the more recent classification of these enzymes into the four groups based on their three-dimensional structure is provided. Although enzymes belonging to these four structural classes have evolved from independent evolutionary lineages, they share some common features near their active sites and in the mode of PLP binding. Earlier work carried out elsewhere on pyridoxal kinase and its key role in maintaining PLP at a low concentration in the cytosol is presented. Different mechanisms that have been proposed for the transfer of PLP from pyridoxal kinase to other apo PLP-dependent enzymes are briefly described. The experimental procedures and computational methods used during the course of these investigations to obtain the results reported in chapters 3-6 are presented in Chapter 2. Most of these methods are applicable to the isolation of plasmids, cloning, over expression, protein purification, mutant construction, crystallization, X-ray diffraction data collection and processing, structure elucidation and refinement, validation and structural analysis presented in the next three chapters. Various programs and protocols used for data processing, structure determination, refinement, model building, structure validation and analysis are also briefly described. In chapter 3, the role of a number of active site residues in the reaction catalyzed by EcDAPAL, a fold type II PLP-dependent enzyme, the structure of which was determined earlier in the laboratory is explored by mutational, biochemical and structural analyses. Earlier studies had established the probable role of Asp120 and Lys77 in the reaction leading to the breakdown of D-DAP and L-DAP, respectively (Bisht et al., 2012). To further validate the earlier observations, a number of active site mutants were generated for Asp 120 (D120N, D120C, D120S and D120T), Asp 189 (D189N, D189C, D189S and D189T), Lys77 (K77T, K77H, K77R and K77A), His 123 (H123L) and Tyr 168 (Y168F). The structure of D120N mutant crystal obtained after soaking in crystallization cocktail containing D-DAP revealed the presence of an intact external aldimine complex at the active site supporting the earlier proposal that Asp120 is the base abstracting the Cα proton from the D-isomer of DAP. Biochemical and structural observations suggested that none of the Asp189 mutants may bind PLP and were catalytically inactive suggesting an essential role for Asp189 in catalysis. In contrast to type I PLP-dependent enzymes, none of the Lys 77 mutants of EcDAPAL could bind PLP either covalently or non-covalently and were inactive with both the isomers of DAP. Thus, Lys77 appears to be important for both PLP binding and catalysis. H123L mutant formed an external aldimine with D-DAP and a gem-diamine complex with L-DAP indicating that this residue is also crucial for catalysis. These studies have provided additional support to the catalytic mechanism of EcDAPAL proposed earlier. The next Chapter 4 explores the structure, function and catalytic mechanism of Salmonella typhimurium DAPAL (StDAPAL). The protein was purified from a construct carrying a hexa-histidine tag at the C-terminus by Ni-NTA chromatography. The purified protein was demonstrated to be homogeneous by SDS-PAGE and MALDI-TOF. Crystals of StDAPAL belonging to the C-centred monoclinic space group (C121) with four molecules in the asymmetric unit were obtained by the micro batch method and used for collecting X-ray diffracting data. The crystal structure was determined by molecular replacement using the homologous enzyme from E. coli (PDB code 4D9M, Bisht et al., 2012), which shares a sequence identity of 50% with the S. typhimurium enzyme as the phasing model in the program Phaser (McCoy et al., 2007) of the CCP4 suite. The model was refined with Refmac5 of CCP4 suite to R and Rfree values of 25.5% and 30.9%, respectively. A superposition of the structure so obtained over EcDAPAL revealed that the two structures are very similar. A sulfate molecule bound to the active site of StDAPAL could be located. The position of the sulfate corresponds to that of the carboxyl group of aminoacrylate intermediate of EcDAPAL (4D9M). The PLP was bound to Lys78 as an internal aldimine. Since the active sites of the two protomers in fold type II PLP-dependent enzymes are independent, it might be possible to obtain functional monomers of EcDAPAL. With this view, mutation of a conserved Trp (Trp399) present in the dimeric interface resulted in the destabilization of the dimeric interface and partial conversion of the dimeric protein to a monomeric protein. However, the monomeric species of EcDAPALW399R was unable to bind PLP and hence did not possess any catalytic activity. This highlights the importance of dimeric organization for efficient binding of PLP as well as for the activity of the enzyme. A remarkable difference between EcDAPAL and StDAPAL is the absence of a disulfide bond between residues Cys271 and Cys299 in StDAPAL equivalent to the bond formed between Cys265 and Cys291 in EcDAPAL. Mutation of Cys265 and Cys291 of EcDAPAL to Ser did not affect the activity of the enzyme towards either of the isomers of the substrate indicating that the disulfide bond is not crucial for enzyme activity. The stability of the loop corresponding residues 261-295 of EcDAPAL was believed to be promoted by the disulfide bond. However, the equivalent loop was found to be ordered in StDAPAL even though the disulfide bond is absent. In contrast to StDAPAL, EcDAPAL did not show any metal dependent activity. The previous two chapters dealt with fold type II PLP-dependent enzymes. In contrast, Chapter 5 deals with revisiting the structure and function of a fold type I PLP-dependent enzyme, Salmonella typhimurium arginine decarboxylase (StADC). ADC is a very large polypeptide in comparison with other fold type I enzymes. It is induced when the bacterium is subjected to low pH and plays a major role in protecting the cells from acid stress. The structure of StADC was determined but not satisfactorily refined by Dr. S. R. Bharat earlier. The X-ray diffraction data collected by Bharat needed to be improved and the structure needed to be further refined and compared with the homologous E. coli enzyme. Therefore, the entire process of data processing, structure solution and refinement was repeated. The refined structure of StADC was found to correspond to the apo form of the enzyme with only a phosphate molecule occupying the position equivalent to that of 5’ phosphate of PLP observed in EcADC holo enzyme structure. This allowed examination of structural changes that accompany PLP binding and formation of an internal aldimine. The apo to holo transition in StADC involves the movement and ordering of two loops consisting of residues 151-164 and 191-196 which are in the linker and PLP binding domains of the protein, respectively. Phosphate binding by itself appears to be insufficient for these structural changes. These two loops are close to the PLP binding site of the other protomer of the dimer. Hence, these movements are probably important for the catalytic function of the enzyme. Holo ADC has been found as a decamer in other studies. The decameric form of the apo-StADC suggests that PLP binding may not be essential for the oligomeric state of the protein. ADC appears to reduce proton concentration inside the cell in two ways; (i) by surface charge neutralization and (ii) by arginine decarboxylation by extracting a proton from the cytoplasm. The resulting product agmatine is exchanged for extra cellular arginine by arginine-agmatine antiporter. The low sequence identity and lack of structural similarity of the inducible and constitutive forms of ADC from S. typhimurium shows that these are unlikely to be products of divergent evolution. The final chapter 6 of the thesis presents the work carried out on S. typhimurium pyridoxal kinase (PLK). In the salvage pathway of pyridoxal 5’phosphate (PLP), PLP is produced as the product of the reaction catalyzed by PLK using PL, PN and PM as substrates. Thus, PLK plays the critical role of ensuring availability of PLP to the large number of PLP-dependent enzymes. S. typhimurium PLK was purified to homogeneity, crystallized in its native as well as ligand bound forms. It was necessary to circumvent an unusual problem caused by spots arising from a contaminant crystal to obtain the structure of the native crystals of PLK that belonged to the P212121 space group with two protomers in the crystal asymmetric unit. It was then straight forward to determine the ligand bound structures of StPLK (space group P43212) obtained by co-crystallization with ATP, PL and Mg2+ by molecular replacement using the wild type structure as the phasing model. The structures obtained by co-crystallization revealed the presence of ADP, Mg2+ and a PL bound to the active site Lys233 via a Schiff base (internal aldimine). This is the first structure in which the presence of an internal aldimine in the active site of PLK has been observed. Formation of the internal aldimine might be one way to prevent the release of excess PLP and protecting the cell from PLP induced toxicity. The enzyme was shown to be inhibited by the product which will also help in maintaining PLP concentration at low levels. It was also demonstrated that PLK interacts with apo-PLP-dependent enzymes. This observation supports possible direct transfer of PLP from PLK to PLP-dependent enzymes. The thesis ends with an appendix where the work carried out during the course of the thesis work but not as part of the thesis is briefly described.
69

Phytomonas serpens: caracterização da piruvato/indolpiruvato descarboxilase e funcionalidade da auxina produzida. / Phytomonas serpens: characterization of the pyruvate/indolepyruvate decarboxylase and functionality of the auxin produced.

Susan Ienne da Silva Vançan 22 May 2012 (has links)
Um gene que codifica uma piruvato/indolpiruvato descarboxilase (PDC/IPDC) está presente no tripanossomatídeo de plantas Phytomonas serpens. A PDC atua na fermentação alcoólica, enquanto que a IPDC atua na biossíntese do fitormônio ácido indol-3-acético (AIA). Análises filogenéticas indicam que a PDC/IPDC de P. serpens é monofilética com IPDCs de gama-proteobactérias, sugerindo um evento de transferência horizontal gênica. A análise de meios de cultura de P. serpens confirma a produção de etanol e AIA. A funcionalidade do fitormônio foi confirmada em ensaios de alongamento de hipocótilos de tomateiros. Tomates inoculados com P. serpens mostraram aumento no teor de AIA-amida e -éster conjugados. A atividade PDC foi mostrada em extratos de P. serpens. Concluímos que a PDC/IPDC seria uma 2-cetoácido descaboxilase com atividade catalítica variável para diferentes substratos. A atividade PDC parece ser predominante em P. serpens, representando um mecanismo para oxidar parte do NADH formado na glicólise, principal responsável pela produção de ATP neste organismo. / A gene codifying a pyruvate/indolepyruvate decarboxylase (PDC/IPDC) is present in the plant trypanosomatid Phytomonas serpens. PDC acts in the alcoholic fermentation, whyle IPDC acts in the biosynthesis of the phytohormone indole-3-acetic acid (IAA). Phylogenetic analysis indicate that P. serpens PDC/IPDC is monophyletic with gamma-proteobacteria IPDCs, suggesting a horizontal gene transfer event. Analysis of P. serpens culture media confirms production of ethanol and IAA. The functionality of the phytohormone was confirmed by tomato hypocotyl elongation tests. Tomatoes inoculated with P. serpens showed an increase in the concentration of IAA amide and ester conjugated. PDC activity was shown in P. serpens extracts. We conclude that the PDC/IPDC would be a 2-keto acid decaboxylase with variable catalytic activity for different substrates. The PDC activity appears to be prevalent in P. serpens representing a mechanism to oxidize part of NADH formed in glycolysis, responsible for ATP production in this organism.
70

Synthesis and evaluation of halogenated amino acid analogues as inhibitors of decarboxylase enzymes of selected pathogens

De Villiers, Jandre 03 1900 (has links)
Thesis (PhD (Chemistry and Polymer Science))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: The use of fluorine in medicinal chemistry has increased dramatically in the last 20 years. The addition of fluorine to a lead compound has various advantages such as the blocking of metabolic active sites, the increase of solubility and lipophilicity of a compound, acting as conformational probes for the active site of an enzyme, and influencing (in most cases increasing) the binding affinity of a compound to a target protein. Their use as mechanism based inhibitors is also well known. In this study we set out to synthesize hydroxyl- and fluorinated-amino acid analogues as potential inhibitors and probes towards the active site of various enzymes. The synthesis of the hydroxylamino acid analogues would precede the fluorinated analogues to serve as precursors with fuorination achieved via a fluoro-dehydroxylation reaction. These aims have successfully been achieved with the synthesis of the two enantiopure isomers of 3-fluoro-aspartic acid. The fluorinated aspartic acid analogues were subsequently used in a conformational analysis, with regards to substrate- and binding activity, which investigated the interaction of these compounds with aspartate decarboxylase (PanD). The synthesis of the 3- hydroxy-analogues of ornithine and diamino pimelic acid was also successfully achieved. These syntheses were done in a stereospecific manner to provide one enantiomer of the L-amino acid analogue. However, our efforts toward the synthesis of the other enantiomer of hydroxy analogues as well as our attempts at the conversion of the hydroxyl group to a fluorine were unsuccessful to date. Nevertheless, these results gave us a new direction towards the synthesis of the desired compounds and have led us to new strategies and ideas. Hopefully, the work done in this study will be part of the ground work towards new methodologies for the synthesis of desired halogenated amino acid analogues as small molecule inhibitors. / AFRIKAANSE OPSOMMING: The use of fluorine in medicinal chemistry has increased dramatically in the last 20 years. The addition of fluorine to a lead compound has various advantages such as the blocking of metabolic active sites, the increase of solubility and lipophilicity of a compound, acting as conformational probes for the active site of an enzyme, and influencing (in most cases increasing) the binding affinity of a compound to a target protein. Their use as mechanism based inhibitors is also well known. In this study we set out to synthesize hydroxyl- and fluorinated-amino acid analogues as potential inhibitors and probes towards the active site of various enzymes. The synthesis of the hydroxylamino acid analogues would precede the fluorinated analogues to serve as precursors with fuorination achieved via a fluoro-dehydroxylation reaction. These aims have successfully been achieved with the synthesis of the two enantiopure isomers of 3-fluoro-aspartic acid. The fluorinated aspartic acid analogues were subsequently used in a conformational analysis, with regards to substrate- and binding activity, which investigated the interaction of these compounds with aspartate decarboxylase (PanD). The synthesis of the 3- hydroxy-analogues of ornithine and diamino pimelic acid was also successfully achieved. These syntheses were done in a stereospecific manner to provide one enantiomer of the L-amino acid analogue. However, our efforts toward the synthesis of the other enantiomer of hydroxy analogues as well as our attempts at the conversion of the hydroxyl group to a fluorine were unsuccessful to date. Nevertheless, these results gave us a new direction towards the synthesis of the desired compounds and have led us to new strategies and ideas. Hopefully, the work done in this study will be part of the ground work towards new methodologies for the synthesis of desired halogenated amino acid analogues as small molecule inhibitors.

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