Thesis (M.S. in Applied Physics)--Naval Postgraduate School, June 2005. / Thesis Advisor(s): William B. Colson. Includes bibliographical references (p. 79-81). Also available online.

Thesis (Ph.D. in Molecular Biology) -- University of Colorado, 2005. / Typescript. Includes bibliographical references (leaves 100-113). Free to UCDHSC affiliates. Online version available via ProQuest Digital Dissertations;

Thesis (M.N.)--Memorial University of Newfoundland, 2004. / Typescript. Bibliography: leaves 92-100.

Thesis (Ph. D.)--University of Washington, 2005. / Vita. Includes bibliographical references (leaves 115-125).

This paper studies the cost effectiveness of climate policy if there are technology externalities. For this purpose, we develop a forward-looking CGE model that captures empirical links between CO2 emissions associated with energy use, directed technical change and the economy. We find the cost-effective climate policy to include a combination of R&D subsidies and CO2 emission constraints, although R&D subsidies raise the shadow value of the CO2 constraint (i.e. CO2 price) because of a strong rebound effect from stimulating innovation. Furthermore, we find that CO2 constraints differentiated toward CO2-intensive sectors are more cost effective than constraints that generate uniform CO2 prices among sectors. Differentiated CO2 prices, through technical change and concomitant technology externalities, encourage growth in the non-CO2 intensive sectors and discourage growth in CO2-intensive sectors. Thus, it is cost effective to let the latter bear relatively more of the abatement burden. This result is robust to whether emission constraints, R&D subsidies or combinations of both are used to reduce CO2 emissions. / Abstract in HTML and technical report in PDF available on the Massachusetts Institute of Technology Joint Program on the Science and Policy of Global Change website (http://mit.edu/globalchange/www/).

Το πρόβλημα εύρεσης συντομότερων διαδρομών είναι ένα από τα πιο θεμελιώδη προβλήματα μονοκριτηριακής βελτιστοποίησης σε δίκτυα. Σε αυτό το πρόβλημα αναζητείται η συντομότερη διαδρομή μεταξύ δύο δεδομένων σημείων ελαχιστοποιώντας ένα κριτήριο κόστους. Σε πολλές εφαρμογές ωστόσο, μας ενδιαφέρουν περισσότερα από ένα κριτήρια προς βελτιστοποίηση. Για παράδειγμα, στην εύρεση διαδρομών σε ένα οδικό δίκτυο με διόδια, μας ενδιαφέρει εκτός από την διανυμένη απόσταση και η ελαχιστοποίηση του χρόνου και του κόστους. Παρόμοια παραδείγματα βρίσκουμε και στον χώρο των δικτύων τηλεπικοινωνιών, όπου εξετάζονται κριτήρια όπως η καθυστέρηση, η πιθανότητα λάθους, ο αριθμός συνδέσμων και άλλα. Σε αυτές τις περιπτώσεις η καλύτερη λύση δεν μπορεί να οριστεί με μονοσήμαντο τρόπο, και συνεπώς καταφεύγουμε σε αντισταθμίσεις μεταξύ των παραγόντων, που είναι γνωστές ως σύνολο λύσεων κατά Pareto. Παρόλο που για το πρόβλημα μονοκριτηριακής εύρεσης συντομότερων διαδρομών υπάρχουν πολλοί αποδοτικοί αλγόριθμοι για την επίλυση του προβλήματος, το αντίστοιχο πολυκριτηριακό πρόβλημα είναι πολύ πιο σύνθετο. Μέχρι τώρα, αυτό το πρόβλημα έχει αποδειχθεί ότι είναι NP-πλήρες. Επιπλέον, έχει αποδειχθεί ότι το πλήθος των λύσεων σε αυτό το πρόβλημα αυξάνεται εκθετικά σε σχέση με το μέγεθος της εισόδου. Υπάρχουν δύο βασικές προσεγγίσεις επίλυσης τέτοιων προβλημάτων, όπου εξετάζονται πολλαπλά κριτήρια. α) Η πρώτη μέθοδος βρίσκει προσεγγιστικές λύσης κατά έναν ορισμένο παράγοντα. Οι προσεγγιστικές μέθοδοι δεν βρίσκουν απαραίτητα ακριβείς λύσεις, αλλά είναι σχετικά γρήγορες και προσφέρουν εγγύηση για το ποσοστό απόκλισης από την βέλτιστη λύση. β) Η δεύτερη μέθοδος χρησιμοποιεί ευρετικές βελτιώσεις για να επιταχύνει τους ήδη υπάρχοντες αλγορίθμους. Τέτοιες τεχνικές βρίσκουν ακριβείς λύσεις, και το ζητούμενο είναι να επιτευχθεί μια πολύ καλή χρονική απόδοση. Στην παρούσα διπλωματική εργασία επικεντρωνόμαστε στην δεύτερη μέθοδο, υποκινούμενοι από την μεγάλη ζήτηση πρακτικών εφαρμογών για εύρεση αποτελεσματικής και ακριβούς λύσης του προβλήματος συντομότερων διαδρομών υπό πολλαπλά κριτήρια. Πιο συγκεκριμένα, στην εργασία αυτή παρουσιάζουμε ένα ενοποιημένο πλαίσιο για την αποδοτική επίλυση αυτών των προβλημάτων. Προτείνουμε νέες μεθόδους ή βελτιώσεις των υπαρχόντων. Υλοποιήσαμε τις μεθόδους που παρουσιάζουμε συνοδεύοντάς τις με μια εκτενή πειραματική μελέτη πάνω σε δίκτυα ευρείας κλίμακας. / We present new implementations of heuristic algorithms for the solution of the multiobjective shortest path problem, using a new graph structure specifically suited for large scale road networks. We enhance the heuristics with further optimizations and experimentally evaluate the performance of our enhanced implementation on real world road networks achieving 10 times better performance with respect to the best previous study.

The research summarized in this thesis focuses on directed evolution and enzyme mechanism studies of two aldolases: 2-deoxyribose-5-phosphate aldolase (DERA) and fructose-6-phosphate aldolase (FSA). Aldolases are nature’s own catalysts for one of the most fundamental reactions in organic chemistry: the formation of new carbon-carbon bonds. In biological systems, aldol formation and cleavage reactions play central roles in sugar metabolism. In organic synthesis, aldolases attract great attention as environmentally friendly alternative for the synthesis of polyhydroxylated compounds in stereocontrolled manner. However, naturally occurring aldolases can hardly be used directly in organic synthesis mainly due to their narrow substrate scopes, especially phosphate dependency on substrate level. Semi-rational directed evolution was used in order to investigate the possibility of expanding the substrate scope of both DERA and FSA and to understand more about the relationship between protein structure and catalytic properties. The first two projects focus on the directed evolution of DERA and studies of the enzyme mechanism. The directed evolution project aims to alter the acceptor substrate preference from phosphorylated aldehydes to aryl-substituted aldehydes. Effort has been made to develop screening methods and screen for variants with desired properties.  In the study of enzyme mechanism where enzyme steady state kinetic studies were combined with molecular dynamic simulations, we investigated the role of Ser238 and Ser239 in the phosphate binding site and the possible connection between enzyme dynamics and catalytic properties. The other two projects focus on the directed evolution of FSA and the development of a new screening assay facilitating screening for FSA variants with improved activity in catalyzing aldol reaction between phenylacetaldehyde and hydroxyacetone. The new assay is based on a coupled enzyme system using an engineered alcohol dehydrogenase, FucO DA1472, as reporting enzyme. The assay has been successfully used to identify a hit with 9-fold improvement in catalytic efficiency and to determine the steady state kinetic parameters of wild-type FSA as well as the mutants. The results from directed evolution illustrated the high degree malleability of FSA active site. This opens up possibilities to generate FSA variants which could utilize both aryl-substituted donor and acceptor substrates.

Extracurricular enterprise activities have steadily increased over the past decade within universities (Rae et al., 2012), as has the domain of entrepreneurial learning research (Wang and Chugh, 2014) yet limited empirical research examines links between the two phenomena. This thesis connects educational theory, entrepreneurial learning theory and entrepreneurial education research to examine the role that extracurricular enterprise activities may have within the entrepreneurial learning processes of students at United Kingdom Higher Education Institutions. Utilising a social constructionist paradigm of enquiry this thesis critically examines perceptions of the value of extracurricular enterprise activities from an educator and student perspective. A semi-structured survey (n=55) and in depth interviews with students (n=23) and enterprise educators (n=3) across 24 UK universities explored what extracurricular enterprise activities students engaged in, their motivations for engagement and the perceived value of extracurricular enterprise activities in relation to entrepreneurial learning processes. Findings suggest that extracurricular enterprise activities not only provide value in the experiential and social learning opportunities afforded for participants, but the positioning of these activities outside of the main curriculum enables students to develop their autonomous learning capabilities. The results contribute to an emerging body of literature examining self-directed learning activities and entrepreneurial learning (Van Gelderen, 2010; Tseng, 2013). The thesis concludes that while experiential and social learning opportunities occupy a central role within entrepreneurial learning processes of university students, self-directed learning activities are increasingly important, and emphasis should be placed upon enabling students to self-direct their entrepreneurial learning processes. For policy and practice, this research provides additional scrutiny of the proposition that extracurricular enterprise activities positively enhance learning through examining what extracurricular enterprise activities students choose to engage in and the benefits they perceive they attained. This research also provides an enhanced understanding of how students interpret and apply the theoretical concept of entrepreneurial learning. Research examining entrepreneurial learning is important in enabling a more effective understanding of the entrepreneurial process yet studies examining student perceptions of entrepreneurial learning remain limited (Mueller and Anderson, 2014; Wang and Chugh, 2014). Finally, this thesis presents the central role of self-directed learning activities to students’ entrepreneurial learning processes and provides recommendations for enhancing entrepreneurial education.

Note from the Library: The fulltext electronic version of this thesis has been removed due to restricted content. Please contact the Unisa Library for more details. / Industrial and Organisational Psychology / M.A. (Industrial Psychology)

A ciclodextrina glicosiltransferase (CGTase) é a única enzima capaz de catalisar a reação de ciclização a partir do amido e, assim, formar oligossacarídeos cíclicos conhecidos como ciclodextrinas (CDs). Através desta reação é produzida uma mistura de α-, β- e γ-CD que, respectivamente, contém 6, 7 e 8 resíduos de glicose. As CDs têm atraído enorme atenção devido ao seu grande potencial de aplicação em diversas áreas da indústria. Potencial este proporcionado por sua estrutura cônica, com interior hidrofóbico, capaz de encapsular sólidos, líquidos e gases, conferindo propriedades importantes e protegendo-os. Neste trabalho foi estudada a imobilização de uma CGTase em sílica mesoporosa de forma direcionada às cisteínas presentes em sua superfície, alterando a exposição do sítio ativo. A ligação via cisteínas nativas da proteína aumentou em quatro vezes a eficiência da imobilização, quando comparada a ligação via grupamento amino. Esta, no entanto, apresentou maior atividade enzimática em faixas mais amplas de temperatura e pH, além de maior estabilidade operacional, mantendo 100 % de sua atividade após 200 h de reação contínua a 60 °C e pH 4. Ainda que apresentando menor estabilidade da ligação, o derivado obtido por ligação dissulfeto manteve 40 % da atividade inicial durante 200 h e então, o suporte pôde ser recarregado e reutilizado por igual período. Os suportes desenvolvidos apresentaram estabilidade satisfatória, possibilitando o uso do derivado imobilizado em reator de leito fixo operado de forma contínua. Quando avaliado em relação a produção das três ciclodextrinas principais, o derivado cuja imobilização da enzima ocorreu via grupamento amino, evidenciou a possibilidade de modulação da produção apenas variando as condições de reação. α- e β-CD foram produzidas preferencialmente em pH 8,0 e 2 min (3,44 mg mL-1 e 3,51 mg mL-1, respectivamente), enquanto que pH mais ácido (4,0) e maior tempo de reação (141 min) favoreceram a formação de γ-CD (3,35 mg mL-1), com baixa formação α-CD (0,75 mg mL-1). Por fim, os resultados deste estudo evidenciam a importância da imobilização da CGTase para a estabilização de sua estrutura a fim de aplicá-la em sistemas contínuos de produção de CDs onde é possível modular o perfil dos produtos gerados em função das condições de reação, aumentando assim a produtividade do biocatalisador. / Cyclodextrin glycosyltransferase (CGTase) is the only enzyme capable of catalyzing the cyclization reaction from the starch and thus forming cyclic oligosaccharides known as cyclodextrins (CDs). Through this reaction, is produced a mixture of α-, β- and γ-CD containing, 6, 7 and 8 glucose residues respectively. Cyclodextrins (CD) have been attracting considerable attention because of its great potential for application in various areas of industry. This potential is provided by its conical structure with hydrophobic interior, capable of encapsulating solids, liquids and gases, changing important features and protecting them. In this work, the immobilization of CGTase in mesoporous silica was studied in a way directed to cysteines present on its surface, altering the exposure of the active site. The connection via native cysteine of the protein increased by four times the efficiency of immobilization compared to amino groups connection. The binding of amino groups, however, showed greater enzymatic activity in wider ranges of temperature and pH, and higher operational stability, while maintaining 100 % of its activity after 200 h of continuous reaction at 60 °C and pH 4. Although showing less stable connection, the derivative obtained by disulfide bond retained 40 % of the initial activity for 200 h and then, the support could be reloaded and reused for the same period. Developed supports showed satisfactory stability, enabling the use of the derivative assets in a packed bed reactor and operated continuously. It was demonstrated the possibility of modulating the CDs production just varying the reaction conditions, using the derivative of which the enzyme immobilization occurred via amino group, to evaluate the production of three main cyclodextrins. α- and β-CD were produced preferentially at pH 8.0 and 2 min (3.44 mg mL-1 and 3.51 mg mL-1, respectively), whereas the more acid pH (4.0) and longer reaction (141 min) favored the formation of γ-CD (3.35 mg mL-1 and 0.75 mg mL-1 of α-CD). Finally, the results of this study show the importance of the immobilization of CGTase to the stabilization of its structure in order to apply it in continuous CD production systems, where it is possible to modulate the profile of the products generated as a function of the reaction conditions, thus increasing the productivity of the biocatalyst.