En pilotanläggning för ultrafiltering testas nu i Norrvattens reningsprocess för att undersöka ifall den kan användas som en tredje mikrobiologisk barriär i reningsprocessen. Målet med detta projekt är att testa membranets kapacitet att filtrera bort viruspartiklar men även membranet generella reduktionsförmåga för andra mikrobiologiska och kemiska kontamineringar. För att hitta lämpliga kandidater att använda sig av för att mäta reduktionskapaciteten av membranet har en litteraturstudie samt experimentell testning av råvattnet genomförts. OD mätningar på bakteriekulturer samt plackbildandeenheter (PBE) har undersökt för att se om bakteriofager kan finnas i proven. Ungefär 9000 L av ingående och utgående vatten från ultrafilteringen har koncentrerats med hjälp av ett elektropositivt filter som senare har eluerats och ultracentrifugerats. Pellet från ultracentrifugeringen har testat för virusdetektion med hjälp av PCR, qPCR samt PBE. TOC och absorbansmätningar har också genomförts på ingående och utgående vatten från ultrafiltermembranet. Slutligen utfördes ett bänkskaleexperiment för att undersöka hur väl filtret reducerade MS2 fager i utgående vatten. Den inledande testningen visade att plantviruset PMMoV och Pseudomonas fager kan vara bra kandidater att använda sig av för att mäta virusreduktionen över ultrafiltermembranet. När elueringen från ultrafiltreringen testades indikerades en minskad DNA koncentrationen över ultrafiltermembranet med hjälp av Qubit-mätningar. Testningen visade även indikation på att PMMoV reduceras över membranet samt att Pseudomonas fager kan finnas i vattnet. TOC och absorbansmätningarna visade en konstant reduktion över membranet. I bänkskaleexperiment borde enlig teori alla fager stoppas av membranet eftersom viruset är större än porstorleken 20 nm, dock visade experimentell testning på att fager även fanns i utgående vatten från filteringen. Resultat av studien indikerar att mikrobiologiska och kemiska kontamineringar tas bort av membranet, dock för att bestämma den exakta virusreduktionen över membranet och ifall alla kontamineringar större än filters porstorlek (20 nm) tas bort kräver vidare testning.  E. coli fager, som i Livsmedelverket nya restriktioner används för att undersöka mikrobiologiska risker i vattenreningsprocesser, har också testats under studien på vattnet utan positiva utslag. Det kan därför vara av intresse att även undersöka andra fager, så som Pseudomonas fager för att kontrollera dem mikrobiologiska riskerna med vattenrening. / The present study was investigating the effectiveness of the ultrafiltration membrane as third biological barrier in Norrvattens drinking water treatment process, using a pilot scale model. This project aims to test the viral reduction capability of the membrane but also to remove other microbiological and chemical contaminants. To find suitable candidates for measuring the reduction capability, literature research has been performed as well as experimental testing of the raw water coming into the treatment plant and the backwash water from the membrane. Bacterial growth analysis using optical density (OD) measurements and plaque forming unit (PFU) has been performed to investigate the presence of bacteriophages. Approximately 9000 L of incoming and outgoing water from the ultrafiltration membrane has been concentrated using an electropositive membrane which then was eluted and ultracentrifuged. The pellet from ultracentrifugation has been tested for viral detection with PCR, qPCR and plating. TOC and absorbance measurement was also performed on the ingoing and outgoing water from the ultrafiltration pilot plant. Finally, a bench-scale experiment was performed using MS2-spiked water to investigate how well the filter reduced MS2 phages in the outgoing water.  The initial testing of the raw and backwash water showed that the plant virus Pepper Mild Mottle Virus (PMMoV) and Pseudomonas phages may be good candidates to use when evaluating the ultrafiltration membrane. When testing the eluate from the ultrafiltration pilot plant a reduction was seen in the starting DNA concentration when comparing the inlet and outlet water to the ultrafiltration pilot plant. The testing gave indications of a reduction of PMMoV and presence of Pseudomonas phages. The bench-scale experiment was hypothesized to stop all viral particles since according to theory the virus should be stopped by the membrane due to its pore size, but experimental testing indicated viruses in the outgoing water from the membrane as well. TOC and absorbance measurements showed a constant reduction over the membrane. The result of the study indicates that microbiological and chemical contaminants are removed by the filter, however, to determine the exact viral reduction potential of the filter and if all contaminant over the size of 20 nm is removed further testing is required.  No indications were seen for Escherichia coli (E. coli) phages in the water throughout the study, which in Livsmedelverket’s (The National Food Agency) new regulations is used for determining the microbiological risks in water treatment processes. It may be of interest to investigate the possibility to also look for other type of phages to determine the microbiological risks, for example Pseudomonas phages which has been seen in this study.

The first part of this doctorate dissertation examines the factors influencing the occurrence and costs of pinhole leak corrosion as well as the household decisions for corrosion prevention and plumbing material selection. Three mail surveys of households were used to elicit the experiences with leaks as well as the optimal corrosion prevention and material choices. Probability modeling (i.e. MNL) and linear regression analysis were used to analyze survey responses. Pinhole leak occurrences were found associated with pipe type installed, property age, pipe failure history, and dwelling distance from a water treatment plant. The number and location of pinhole leaks in the dwelling and the pipe type are associated with the financial costs of pinhole leaks. The corrosion prevention choices as well as the plumbing materials depended on the risk of corrosion and cost associated with each option. Previous experiences with pinhole leak impacted the decision for household choices. Faster responses to pinhole leak outbreaks by utility managers and policymakers in terms of advising homeowners on the best ways of responding to leaks would assist homeowners in reducing costs of pinhole leak repairs and associated damages. The second part of this document deals with the debt financing issues. Debt financing decisions are made simultaneously by lenders and borrowers. Since lenders are unable to observe directly the firms’ investment decisions, the banks offer contracts based up on firms’ observable characteristics (i.e. wealth and size) and the prevailing market conditions. When deciding on the financing decisions, firms also take into account the changes in macroeconomic variables in order to lower the cost of borrowing. As a result, the goal for this article is to examine empirically the hypothesis of the effect of the debt determinant as well as the macroeconomic variables on the debt maturity structure. A reduced form of the simultaneous financing decisions model is estimated by employing several OLS estimation methods. The empirical findings offer strong support for firms with few growth options, large, and of low quality having more long-term debt in their capital structure. There was, however, no clear support for the impact of macroeconomic variables on debt maturity as some variables were not statistically significant. / Ph. D.

The United Nations established Sustainable Development Goal 6, universal access to safely managed drinking water and sanitation service, as a global goal for 2030. In rural areas, access lags significantly and progress is rarely examined concurrently between developed and developing nations. Therefore, this dissertation focuses on rural water system challenges in a developed nation, the US, and a developing nation, Guatemala. In the US, approximately 250 million Americans receive drinking water from community water systems (CWSs), theoretically safeguarded by the Safe Drinking Water Act (SDWA). There is mounting evidence that racial, ethnic, and socioeconomic disparities persist in US drinking water access and quality, but studies are limited by the exclusion of very small CWSs and a large geographic unit of analysis. A novel geospatial methodology was created to delineate system service areas at the zip code scale in Virginia and assess the influence of demographic characteristics on compliance with the SDWA from 2006 to 2016. Results reveal that monitoring and reporting violations are concentrated in private, rural systems that serve fewer than 500 people, while health-based violations were more likely in non-white communities, specifically those with higher proportions of Black, Native Hawaiian, and other Pacific Islanders. This study was completed in parallel with a household sampling campaign in rural San Rafael Las Flores, Guatemala. In Guatemala, no public access to water system compliance or quality information currently exists. With growing investment in mining industries and recognized naturally occurring arsenic in volcanic geology, citizens are eager for drinking water information. Survey results highlighted dissatisfaction with and distrust in most tap water sources. Consequently, residents regularly buy bottled water or collect water from untreated natural springs. Water quality results indicated that tap water from the central drinking water treatment plant contained higher levels of arsenic and other contaminants, when compared to most other sources. Though the settings are quite different, parallel investigation of rural drinking water system challenges in the US and Guatemala reveal common challenges and lessons. Moving forward, all nations would benefit from standard monitoring of drinking water access, quality, and compliance that allowed for intersectional investigations of environmental health inequities. / Doctor of Philosophy / In 2015, the United Nations established Sustainable Development Goal 6 which establishes safely managed drinking water and sanitation service for all as a global goal. Access to safe drinking water lags significantly in rural areas and can be complicated by intersecting social determinants of health (e.g. race, wealth). Rarely is progress in developed and developing nations examined concurrently, hindering an understanding of commonalities and an exchange of lessons. To this end, my dissertation focuses on rural water system challenges in a developed nation, the United States, and a developing nation, Guatemala. In the US, more than 250 million Americans receive in-home drinking water from one of 53,000 community water systems, with quality theoretically protected by the Safe Drinking Water Act (SDWA). Recent failures, such as the lead crisis in Flint, MI, have cast doubt on the equity and reliability of these utilities, especially in underserved areas. How can we ensure that all US communities receive equal protections under the Safe Drinking Water Act? Using publicly available data and geography, this work estimated service areas to determine whether SDWA violations related to surrounding community socio-demographics and/or system design. Results reveal that monitoring and reporting violations are significantly concentrated in private, rural systems that serve fewer than 500 people, while health-based violations were more likely in non-white communities, specifically those with higher proportions of Black, Native Hawaiian, and other Pacific Islanders. These findings illustrate potential issues of environmental justice within VA and advocate for future research to investigate potential structural causes. This work was completed in tandem with a household sampling campaign in rural San Rafael Las Flores, Guatemala. In Guatemala, there is currently no public access to water system compliance or quality information. With recognized naturally occurring carcinogenic elements in Guatemala's volcanic geology, such as arsenic, and heightened investment in extractive industries such as mining, that can compromise source water quality, citizens are eager for drinking water quality data. Survey results documented widespread dissatisfaction with and distrust in tap water quality. As a consequence, residents regularly buy bottled water or collect water from natural springs. Water quality results showed that tap water sourced from the central drinking water treatment plant contained significantly higher levels of arsenic and other contaminants when compared to most other tap sources. Community participation in long-term water monitoring and infrastructure decisions may help build trust in water sources. Though the regulatory, economic, and cultural settings are quite different, parallel investigation of rural drinking water system challenges in the US and Guatemala reveal common challenges and lessons. Moving forward, high, middle, and low-income nations all benefit from standard monitoring of drinking water access, quality, and compliance that allows for intersectional investigations of environmental health inequities.

Manganese is a problem at the Ni River Water Treatment Plant in Spotsylvania County, Virginia. The Ni River Reservoir (the water source) is a eutrophic reservoir. In the summer, the dissolved oxygen decreases to near or zero at depths greater than two meters. As a result, soluble manganese increases to levels of nearly 6.0 mg/L at the bottom. It is released from the sediments under anaerobic conditions. Total organic carbon levels ranging from 4.0 to 7.25 mg/L were noted with increasing depth. Plant profiles were developed to indicate the performance of the sedimentation and filtration units in reducing manganese concentration. Essentially, all the particulate manganese was removed by sedimentation, and some removal of soluble manganese was evident. The filters removed additional soluble manganese. Soluble manganese removal probably was due to the adsorption of manganese on solid manganese dioxide in the sludge blanket and on the filter media with subsequent further oxidation. Ozone was effective at a dose of approximately 5 mg/L. Chlorine and chlorine dioxide were marginally effective as pretreatments at dosages of 5 and 2 rng/L, respectively. Potassium permanganate proved effective at dosages of 0.5 to 0.625 mg/L (1.5 to 1.9 times the theoretical requirement). Aeration proved effective in reducing levels of approximately 0.1 mg/L to below the secondary maximum contaminant level (0.05 mg/L) and, in addition, somewhat effective in reducing a concentration of nearly 2 mg/L by 31 percent. Aeration appears to be a viable means of reducing the anaerobic conditions in the reservoir that lead to the high soluble manganese concentrations. / M.S.

Tesis por compendio / [ES] En la presente tesis doctoral se ha evaluado el uso de nuevos soportes celulósicos y silíceos como sistemas de filtración para la estabilización y conservación de alimentos líquidos con el fin de afrontar dos grandes retos de la industria de bebidas. Por un lado, evitar o minimizar los cambios en las propiedades nutricionales, estructurales y organolépticas de los alimentos, ocasionados por la pasteurización térmica tradicional, y ofrecer una alternativa al problema de la baja viabilidad debida a los altos costos de inversión/producción al aplicar nuevas tecnologías no térmicas. Por ello, esta tesis doctoral se centra en el desarrollo y evaluación de una nueva tecnología no térmica de conservación de alimentos líquidos basada en la filtración. Se han desarrollado sistemas de filtración a partir de soportes celulósicos y silíceos, sin funcionalizar o funcionalizados con compuestos antimicrobianos. En el primer capítulo se evaluó el uso de materiales de celulosa como soportes filtrantes para el tratamiento de alimentos líquidos. Como primera aproximación se desarrolló un material poroso nano-micro tubular a partir de la extracción y deslignificación del material celulósico presente en el corazón o raquis de la mazorca de maíz. El uso de este soporte resultó ser efectivo como material filtrante para el tratamiento de agua y zumo de naranja, en un sistema de flujo continuo, eliminando la carga microbiana. La aplicación de este soporte como sistema de filtración presenta diversas ventajas como su capacidad de retención microbiana, la reutilización de sub-productos del maíz y, por tanto, su respeto al medioambiente. Sin embargo, sería necesario optimizar el proceso de filtrado para evitar la frecuente obturación de sus poros que requirió varios ciclos de lavado durante el proceso, así como establecer un método de regeneración del material para incrementar su vida útil. Además, este sistema afectó al color del zumo filtrado, que no se mantuvo constante durante el proceso, lo que supone una importante desventaja que es necesaria abordar. Como segunda aproximación, se evaluó el potencial de la inmovilización de una molécula bioactiva sobre membranas de celulosa, para mejorar la capacidad de retención microbiana del material celulósico, así como permitir su reutilización. Los filtros de celulosa funcionalizados con poliaminas demostraron ser eficaces en la eliminación de patógenos en agua, debido a las cargas positivas generadas por los grupos amina inmovilizados en la superficie de las membranas, que atraen y retienen las bacterias cargadas negativamente. Dada la fácil preparación y procedimiento de uso de las membranas de celulosa funcionalizadas con poliaminas, éstas podrían ser consideradas una buena opción para el desarrollo de sistemas de tratamiento de aguas in situ, rápidos, de fácil manejo y de bajo coste. El segundo capítulo describe el desarrollo y aplicación de partículas de sílice funcionalizadas con compuestos de aceites esenciales, con el fin de diseñar coadyuvantes de filtración con actividad antimicrobiana. La filtración de diversas matrices alimentarias (agua, cerveza y zumo de manzana) a través de los soportes funcionalizados con los antimicrobianos naturales demostró ser eficaz en la reducción del recuento de la cepa patógena Escherichia coli, así como frente a la microflora endógena de la cerveza y el zumo (bacterias acidolácticas, aerobios mesófilos, psicrófilos, mohos y levaduras). La eficacia en el control microbiano se debe a la combinación de la adsorción física y la inactivación por contacto con los compuestos de aceites esenciales inmovilizados. Además, la evaluación de las propiedades físico-químicas y sensoriales de los alimentos líquidos demostró un efecto poco significativo, éste depende del tamaño de las partículas de sílice usadas y de la molécula bioactiva inmovilizada. Por lo tanto, el sistema de conservaci� / [CA] En la present tesi doctoral s'ha avaluat l'ús de nous suports cel·lulòsics i silicis com a sistemes de filtració per a l'estabilització i conservació d'aliments líquids, amb la finalitat d'afrontar dos grans reptes de la indústria de begudes. D'una banda, evitar o minimitzar els canvis en les propietats nutricionals, estructurals i organolèptiques dels aliments, ocasionats per la pasteurització tèrmica tradicional, i oferir una alternativa al problema de la baixa viabilitat deguda als alts costos d'inversió/producció en aplicar noves tecnologies no tèrmiques. Per això, aquesta tesi doctoral es centra en el desenvolupament i avaluació d'una nova tecnologia no tèrmica de conservació d'aliments líquids basada en la filtració. S'han desenvolupat sistemes de filtració a partir de suports cel·lulòsics i silicis, sense funcionalitzar o funcionalitzats amb compostos antimicrobians. En el primer capítol es va avaluar l'ús de materials de cel·lulosa com a suports filtrants per al tractament d'aliments líquids. Com a primera aproximació es va desenvolupar un material porós nano-micro tubular a partir de l'extracció i deslignificació del material cel·lulòsic present en el cor o raquis de la panolla de dacsa. L'ús d'aquest suport va resultar ser efectiu com a material filtrant per al tractament d'aigua i suc de taronja, en un sistema de flux continu, eliminant la càrrega microbiana. L'aplicació d'aquest suport com a sistema de filtració presenta diversos avantatges com la seua capacitat de retenció microbiana, la reutilització de subproductes de la dacsa i, per tant, el seu respecte al medi ambient. No obstant això, seria necessari optimitzar el procés de filtrat per a evitar la freqüent obturació dels seus porus que va requerir diversos cicles de rentada durant el procés, així com establir un mètode de regeneració del material per a incrementar la seua vida útil. A més, aquest sistema va afectar el color del suc filtrat, que no es va mantenir constant durant el procés, la qual cosa suposa un important desavantatge que és necessari abordar. Com a segona aproximació, es va avaluar el potencial de la immobilització d'una molècula bioactiva sobre membranes de cel·lulosa, per a millorar la capacitat de retenció microbiana del material cel·lulòsic, així com permetre la seua reutilització. Els filtres de cel·lulosa funcionalitzats amb poliamines van demostrar ser eficaces en l'eliminació de patògens en aigua, a causa de les càrregues positives generades pels grups amina immobilitzats en la superfície de les membranes, que atrauen i retenen els bacteris carregats negativament. Donada la fàcil preparació i procediment d'ús de les membranes de cel·lulosa funcionalitzades amb poliamines, aquestes podrien ser considerades una bona opció per al desenvolupament de sistemes de tractament d'aigües in situ, ràpids, de fàcil maneig i de baix cost. El segon capítol descriu el desenvolupament i aplicació de partícules de sílice funcionalitzades amb compostos d'olis essencials, amb la finalitat de dissenyar coadjuvants de filtració amb activitat antimicrobiana. La filtració de diverses matrius alimentàries (aigua, cervesa i suc de poma) a través dels suports funcionalitzats amb els antimicrobians naturals va demostrar ser eficaç en la reducció del recompte del cep patogen Escherichia coli, així com enfront de la microflora endògena de la cervesa i el suc (bacteris àcid làctics, aerobis mesòfils, psicròfils, floridures i llevats). L'eficàcia en el control microbià es deu a la combinació de l'adsorció física i la inactivació per contacte amb els compostos d'olis essencials immobilitzats. A més, l'avaluació de les propietats fisicoquímiques i sensorials dels aliments líquids estudiats va demostrar un efecte poc significatiu, aquest depèn de la grandària de les partícules de sílice usades i de la molècula bioactiva immobilitzada. Per tant, el sistema de conserv / [EN] In the present doctoral thesis the use of new cellulosic and silica supports as filtering systems for the stabilization and preservation of liquid foods has been evaluated to overcome two major challenges of the beverage industry. On the one hand, avoid or minimize the changes in the nutritional, structural and organoleptic properties of food caused by traditional thermal pasteurization, and offer an alternative to the problem of low viability due to high investment/production costs when applying new non-thermal technologies. Therefore, this doctoral thesis focuses on the development and evaluation of a new non-thermal technology for the preservation of liquid foods based on filtration. The filtering systems have been developed from cellulosic and silica supports, non-modified or functionalized with antimicrobial compounds. In the first chapter, the use of cellulose materials as filtering supports for the treatment of liquid foods was evaluated. As first approximation, a porous nano-micro tubular material was developed from the extraction and delignification of the cellulosic material present in the corn stalk. The use of this support was effective as filtering material for the treatment of water and orange juice, in a continuous flow system, eliminating the microbial load. The application of this support as filtering system has several advantages, such as its microbial retention capacity, the reuse of corn by-products and, therefore, its respect for the environment. However, it would be necessary to optimize the filtering process to avoid the frequent clogging of its pores that required several washing cycles during the process, as well as to establish a method of material regeneration to increase its life. In addition, this system affected the color of the filtered juice, which did not remain constant during the process, representing an important disadvantage that must be addressed. As a second approach, the potential of the immobilization of a bioactive molecule on cellulose membranes was evaluated to improve the microbial retention capacity of the cellulosic material, as well as to allow its reuse. The cellulose filters functionalized with polyamines proved to be effective in eliminating pathogens in water, due to the positive charges generated by the amine groups immobilized on the surface of the membranes, which attract and retain the negatively charged bacteria. Given the easy preparation and usage of the polyamines-functionalized cellulose membranes, these could be considered a good option for the development of fast, easy to use and low cost in situ water treatment systems. The second chapter describes the development and application of silica particles functionalized with essential oil components to design filtering aids with antimicrobial activity. The filtration of various food matrices (water, beer and apple juice) through the supports functionalized with natural antimicrobials proved to be effective in reducing the load of the pathogenic strain Escherichia coli, as well as reducing the endogenous microflora of beer and the juice (lactic acid bacteria, mesophilic, psychrophilic, mold and yeast). The removal capability is due to the combination of physical adsorption and contact inactivation with the essential oil compounds immobilized. In addition, the evaluation of the physicochemical and sensory properties of the liquid foods studied showed a not significant effect, it depends on the size of the silica particles used and the immobilized bioactive molecule. Therefore, the proposed preservation system has a high potential for cold beverage pasteurization processes. / N. Peña-Gomez would like to thank for financial support in the frame of her PhD project to Operational Programme of the European Social Fund (ESF) 2014-2020, the Agencia Estatal de Investigación, Generalitat Valenciana and FEDER-EU (Projects RTI2018-101599-B-C21 and AGL2015-70235-C2-1-R). The authors also thank the Electronic Microscopy & Microanalysis Laboratory at Patras University for support. / Peña Gomez, N. (2020). Development of polymeric and silica filtering materials functionalized with antimicrobial compounds for the elimination of microorganisms in liquid food [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/137041 / Compendio

Drinking water reservoirs are an important water source in many regions around the globe. As man-made structures, they are actively managed to provide raw water of adequate quality. Global change, and in particular global warming, is influencing the available water quantity and quality and is putting pressure on the management of drinking water reservoirs. To adapt management to the changing conditions, tools to evaluate different measurements, as well as predictions of potential future conditions, are required. Mechanistic models are the tool of choice to test adaptation measures and estimate potential future conditions. In this thesis, the impact of climate warming on the water quality of drinking water reservoirs, as well as potential mitigation strategies are investigated using one-dimensional mechanistic lake models. Therefore, three reservoirs located in Saxony, Germany are chosen as study sites. Specifically, the thesis evaluates the potential of adapting the withdrawal depth to mitigate the impact of global warming onto the water quality of reservoirs. To estimate the uncertainty associated with the used models, a framework for ensemble modeling of lakes and reservoirs is developed and applied to simulate climate impact predictions for a drinking water reservoir. The main findings of this thesis are: Adapting the withdrawal depth has the potential to mitigate some impact of global warming. Especially, the simulated impact of withdrawal depth on deep water temperature was about the same magnitude as the observed impact of global warming in the last 30 years. By using an ensemble approach, different sources of uncertainty were quantified and compared. For the climate impact simulation the largest uncertainty was found to be the epistemic uncertainty, which is related to the model structure. Nevertheless, the estimated trends for the climate scenario from all five applied models were coherent. The thesis furthers our knowledge of drinking water reservoir management in a warming climate. Using mechanistic models and ensemble techniques were shown to be an effective tool to compare different management strategies and evaluate uncertainties related to the modeling process. Further mitigation measures need to be developed to safeguard drinking water production under global warming, and methods presented in this thesis help to better evaluate strategies for managing drinking water reservoirs.:1 Introduction 1.1 Background 1.1.1 Down by the river: Drinking water reservoirs 1.1.2 Take care of business: Impact of management 1.1.3 All the world is green: Phytoplankton and lake physics 1.1.4 The times they are a-changing: Climate warming 1.1.5 OK computer: Process-based models 1.2 Study area 1.2.1 Investigated reservoirs 1.2.2 Local impact of climate warming 1.3 Aims and structure of the thesis 1.3.1 Research questions 1.3.2 Structure of the thesis 2 Managing climate change in drinking water reservoirs: potentials and limitations of dynamic withdrawal strategies 2.1 Background 2.2 Methods 2.2.1 Study area 2.2.2 Model input 2.2.3 Model setup 2.2.4 Management strategies 2.2.5 Statistical evaluation 2.3 Results 2.3.1 Observed trend 2.3.2 Validation of simulated temperature profiles 2.3.3 Modeled impact of management 2.3.4 External and internal forcing 2.4 Discussion 2.4.1 Observed trends in hydrophysical features 2.4.2 Modeled impact of management 2.4.3 Statistical model 2.4.4 Climate change and implications for management 2.5 Conclusion 3 LakeEnsemblR: An R package that facilitates ensemble modelling of lakes 3.1 Introduction 3.2 Methods 3.2.1 Model description 3.2.2 R package description 3.2.3 Getting started 3.2.4 Calibration algorithms 3.2.5 Combining multiple ensemble runs 3.3 Example application of LakeEnsemblR 3.3.1 Lough Feeagh: water temperature dynamics 3.3.2 Langtjern: lake ice dynamics 3.3.3 Uncertainty partitioning 3.3.4 Multi-parameter ensemble 3.3.5 Discussion 3.4 Summary 3.4.1 Framework 3.4.2 Recommendations for use 3.4.3 Outlook 4 Ensemble of models shows coherent response of a reservoir’s stratification and ice cover to climate warming 4.1 Introduction 4.2 Methods 4.2.1 Study site 4.2.2 Climate scenarios and data 4.2.3 Lake model ensemble 4.2.4 Calibration 4.2.5 Data evaluation 4.2.6 Uncertainty partitioning 4.3 Results 4.3.1 Observed trends 4.3.2 Model performance 4.3.3 Climate predictions 4.3.4 Uncertainty partitioning 4.4 Discussion 4.5 Conclusions 5 From T to P: Impact of withdrawal on water quality of a drinking water reservoir 5.1 Introduction 5.2 Methods 5.2.1 Study site and data 5.2.2 Applied models 5.2.3 Model calibration and sensitivity analysis 5.2.4 Withdrawal strategies 5.3 Results 5.3.1 Model calibration 5.3.2 Withdrawal strategies 5.4 Discussion 5.5 Conclusion 6 Synthesis 6.1 Lessons learned 6.1.1 Impact of global warming on reservoir water quality 6.1.2 Mitigation through adaptive withdrawal strategies 6.1.3 Using models for scenario analysis 6.2 Next steps 6.2.1 Further management and climate simulations 6.2.2 Model based real time decision support 6.2.3 Improve biogeochemical models 6.3 Concluding remarks A Appendix Chapter 2 A.1 Additional plots A.2 Principal component analysis A.3 Full linear model B Appendix Chapter 3 B.1 Format input files B.2 Additional figures B.3 Additional tables C Appendix Chapter 4 C.1 Additional Tables C.2 Additional Figures D Appendix Chapter 5 D.1 rodeoFABM D.2 Model description D.2.1 State variables D.2.2 Processes D.2.3 Parameter D.2.4 Stoichiometry D.3 Sensitivity analysis D.4 Additional Tables D.5 Additional Figures

During 20th century main concern was to have sanitary water flowing through the tap. In 21st century constant supply of safe drinking water is common at any home in USA. Hence consumers pay attention to aesthetic quality of tap water. Odorous algal metabolites in source water and metals introduced to drinking water due to corrosion of pipes in the distribution system cause taste, odor and color problems, and result in complaints and perception of tap water as unhealthy. Millions of dollars are spent each year by water industry to address and prevent these issues. This research focused on some of the taste-and-odor issues associated with drinking water. First aim was to understand when geosmin, 2-MIB, and nonadienal become detectable, employing two-resistance mass transfer theory to determine the concentration of odorants in bathroom air. Results showed that water temperature and odorant concentration in water play an important role. Next focus was to develop an international odor standard to be used for training of sensory analysis panelists. There are many sensory methods to monitor drinking water to detect the off-flavors however an odor standard has been missing. Hexanal was studied with trained flavor profile analysis panels and was proposed as an ideal odor reference standard to be used for training and sensory assessment of water samples. Main focus was to understand metallic flavor of drinking water caused by iron and copper. It was shown that metallic sensation has taste and retronasal components creating the flavor and humans are very sensitive to it. Occurrence of lipid oxidation in the oral cavity was shown when metals were ingested, that produces carbonyls which are responsible for the metallic flavor. Antioxidants and chelators were investigated to study prevention of lipid oxidation and, chelators were determined to be more effective. Oral epithelial cell cultures were developed as a model for oral cavity to further investigate lipid oxidation and effectiveness of the antioxidants and chelators. This dissertation is a result of inter-disciplinary work and possibly a good example for how problems may be solved by incorporating different methods and point of views from several disciplines. / Ph. D.

There are rural residents of Central Appalachia that collect their drinking water from roadside springs despite having access to in-home piped point-of-use (POU) water. Residents have cited perceptions about water availability/quality as primary motivators for collecting drinking water from roadside springs. Water from roadside springs has been found to contain total coliform and E. coli, suggesting that consumers may be at an increased risk of contracting gastrointestinal illnesses. This research effort seeks to better understand roadside spring usage in Central Appalachia, by exploring motivations influencing potable water source selection and comparing household and spring water quality to Safe Drinking Water Act recommendations. Households were recruited from communities surrounding springs in three states (Kentucky, Virginia, and West Virginia). 24 tap water samples were collected from participating households and paired with samples from six roadside springs. Samples were analyzed for fecal indicator bacteria and inorganic ions. Study participants also completed short surveys to inventory their perceptions of their household drinking water. The majority of participants did not trust their home tap water, indicating water aesthetics as primary motivators for distrust of their homer water source. Statistical comparisons indicated that 10 water quality constituents (Cd, F, NO3-, Cu, Pb, Ag, Mn, Zn, Na, and Sr) were significantly higher in tap water samples and four constituents (total coliform, U, Al, and SO2-4) were significantly higher in spring samples. These results suggest that residents might be exposed to different risks based on their drinking water source and that water quality solutions must be devised case-by-case. / Master of Science / Some rural residents of Central Appalachia collect their drinking water from roadside springs, despite having access to piped drinking water at their homes. Water collected from roadside springs can contain harmful bacteria, suggesting that people may risk exposure to illness when consuming spring water. Through a household water quality study, this research effort aims to compare roadside spring and in-home tap water quality in order to determine what contaminants are present at each source, and why residents are choosing spring water over tap water. Households were recruited from communities surrounding roadside springs in three states (Kentucky, Virginia, and West Virginia). A total of 24 tap water samples were collected from participating households, and compared with samples from six nearby roadside springs. Samples were analyzed for bacteria, metals, and nutrients. Study participants were also asked to complete short surveys to better understand their perceptions of their drinking water sources. The majority of participants did not trust their home tap water, citing aesthetic concerns as the primary reason behind their distrust. When comparing roadside spring and home samples, 10 contaminants (Cd, F, NO3-, Cu, Pb, Ag, Mn, Zn, Na, and Sr) had greater concentrations in home tap water samples and four (total coliform, U, Al, and SO2- 4) had greater concentrations in spring samples. While home water samples had higher levels of metals, roadside spring samples had higher levels of bacteria, suggesting that residents might be exposed to different risks based on water source and that water quality solutions must be developed case-by-case.

Most of the US population is served by large-scale, centralized drinking water, wastewater, and storm water systems built in the late 19th and early 20th century. Multi-trillion dollar investments are needed over the next 20 years to restore failing infrastructure, expand service areas to accommodate growing populations, and meet rising service provision costs driven by environmental and regulatory factors. A formal plan that recognizes the socioeconomic complexity of water services provision in the US is needed to guide these investments. Rising residential water charges over the last 20 years have raised concerns about household affordability of basic water services. Meanwhile, anywhere from 9 to 45 million people (4-28% of the US population) are affected by health-based drinking water quality violations each year. New technologies and evolving social goals have the potential to reshape the sector as we know it. This dissertation is a first attempt at synthesizing the sub questions around which spending is most critical and how rates can be structured for more equitable outcomes. Research on the determinants of historic charge growth has been hindered by a lack of long-term longitudinal data. Unlike energy and telecom utilities, less than 20% of all drinking water utilities (primarily the investor-owned utilities) are economically regulated by public utility commissions. Absent local requirements, municipal water systems whose operating revenues are insufficient to cover operating expenses can make up for budget shortfalls with net transfers from the city general fund. We combine 10 American Water Works Association (AWWA) rate surveys to construct an 18-year unbalanced panel of charges at 446 large utilities (i.e., population served ≥10,000), revealing elevated 2-year compound annual charge growth (CAGR) between 2008 and 2012. We estimate heterogenous impacts of the Great Recession on CAGR with an event study design of 204 utilities, comparing those with 2007 operating ratios below 1.2 (PCR) to those with ratios of ≥ 1.2 (FCR). Despite having the same cumulative 18-year CAGR, 2009-2013 CAGR was 3.9, 5.2, 5.2, and 5.7 percentage points per year higher at PCR utilities for 500, 1,000, 1,500, and 3,000 cf/month, respectively. Our findings demonstrate that large utilities who were likely relying on the general fund at the start of the Great Recession had to sharply increase their charges in the short-term to make up for decreased availability of that funding source. This indicates more immediate affordability challenges at utilities with operating ratios below 1.2 in the event of a similar economic shock. Additional data is needed to evaluate generalizability of these results to smaller systems. Willingness to pay (WTP) for improved drinking water quality is estimated based on the cost of household averting behaviors taken during violations. While buying bottled water is a well-documented reaction to these violations, demographic and socioeconomic differences in water intake, preferred water supply alternatives, and shopping behavior suggest heterogeneity in the timing and form (e.g. soda vs bottled water) of household responses. We quantify this heterogeneity with an event study design using monthly household purchases of soda, water, and juice from the Nielsen Homescan Consumer Panel Dataset from 2004 through 2017. We find that, while households spend an average 14.8% more on bottled water during a month with a nitrate violation, these responses are concentrated in non-hispanic (NHP) Black and NHP Other households below the poverty threshold. Bottled water spending is 91.8% higher than usual in violation months for the former group and 90.0% higher than normal in the month after a violation for the latter. A simultaneous 103% increase in juice spending results in a 30.6% increase in combined beverage spending for NHP Black households below the poverty line in a violation month. Meanwhile, an 85.3% increase in soda spending at NHP Other households drives a 58.9% increase in combined beverage spending in months with a violation and a 134% increase in soda spending, along with increased bottled water purchases, results in a 106% increase in combined beverage spending the month after a violation. NHP Black households with an IPR between 1 and 2 spend 129% more on soda during a violation. Our results indicate that studies focused only on bottled water purchases have systematically undercounted the effects on Hispanic, NHP Black, and Other NHP American households below the poverty threshold in their measure of WTP for improved drinking water quality or for the true cost of violations. Additional analysis is needed to determine if the tap water substitutions made by Hispanic households and households below the poverty threshold are increasing their overall sugary beverage consumption, which can have negative long-term health effects that are also not being considered. Finally, we explore the ongoing implementation of onsite and distributed water reuse systems (ODWRS) to better understand how sociotechnical transitions can be supported when they are deemed to be a socially optimal solution. ODWRS collect previously discarded water flows as alternative water sources (e.g. rainwater, stormwater, greywater, wastewater) from one or more buildings and treat that water close to the point of generation or point of use to a quality deemed safe for its intended end use. Widespread adoption of ODWRS requires both acceptance of new technologies (e.g., water treatment, engineering) and alterations to established social systems(e.g., norms, stakeholder engagement, regulations). This has often been discussed as a single transition for all types of systems at the same time. However, the types of ODWRS being implemented in different areas are not the same. We compile and analyze the first national dataset of system-level information on ODWRS to identify clusters of system types associated with specific drivers. We find evidence of multiple transitions based on the combination of original source contamination level and end use application contact level. Low-contamination, low-contact are almost in the final stage of sociotechnical transition for many locations. Many areas with water supply constraints have already begun the transition for high-contamination to low- or medium-contact systems. Wastewater ODWRS are being implemented in places with effluent disposal constraints and stormwater ODWRS are being implemented in areas with combined sewers or high flood risk, though usually only for low-contact end uses.

Vad för förutsättningar ges det för aktörer i Sverige som ska implementera policies rörande dricksvatten? Är det styrning från sådd till skörd för att nå de uppsatta direktiven eller är det upp till aktörerna att hitta de lösningar som är bäst lämpade till de geografiska förutsättningarna som skiljer sig åt över Sverige? I intervjuer med en dricksvattenexpert och en projektledare inom dricksvattenarbete ges insikter om hur ett policydirektiv på EU-nivå kan anpassas efter lokal, regional och nationell nivå, genom samverkan från individers olika erfarenheter och tolkningar av direktivet, så kallad policy mobility. För att se om nuvarande dricksvattendirektiv kan förstås utifrån begreppet policy mobility genomfördes en dokumentanalys av direktivet och offentliga dokument som gäller implementering av den i Sverige. Det går att utläsa att de offentliga dokumenten öppnar upp för en implementeringsmetod som policy mobility då direktivet är förhållandevis generellt, och förutsätter viss anpassning på nationell såväl som lokal och regional nivå. EU använder sig av territoriell styrning vilket skapar förutsättning för anpassningar för direktiv då det lyfter att geografiska förutsättningar skiljer sig åt mellan medlemsstater vilket innebär att ett sätt inte fungerar likadant överallt. Den lyfter även att det skiljer medlemsstaterna åt om vilken nivå, och hur, beslut kan tas på, som ett förtydligande av subsidiaritetsprincipen. För att kunna implementera dricksvattendirektivet krävs en anpassning till specifika geografiska förutsättningar. Dricksvattendirektivet är ett minimidirektiv, ett antal mål ställs upp men ingen vägledning till hur de ska uppnås medan medlemsstater får rätt till att själva sätta högre mål. Denna uppsats visar att policy mobility har varit närvarande vid arbetet av implementering av dricksvattendirektivet.