A research report submitted to the Faculty of Engineering an the built environment, University of the Witwatersrand, Johannesburg in partial fulfilment of the requirements for the degree of Master of Science in Engineering. Johannesburg, October 2016. / The study investigates the effectiveness of Rand Water’s Scientific Services’ skills development strategy for the assurance of quality drinking water as prescribed by the SANS 0241 National Drinking Water Quality Standard. The aim is to establish whether: 1) the present skills are adequate to provide the scientific data required for affirming drinking water quality and 2) the skills development taking place in the Scientific Services division is adequate for the level and quantity of scientific skills required for the future. There is also some discussion to understand the motivation for maintaining and increasing skills within the Scientific Services division for Rand Water. Assuring drinking water quality within Rand Water is the sole responsibility of the Scientific Services division. The division provides regular routine and non-routine drinking water quality monitoring, testing, data collection, analyses and reporting on the organisation’s performance against the SANS 0241 Drinking Water Quality Standards (SANS, 2006).The focus of the analysis is Scientific Services Division in Rand Water, although the discussion in view of the topic is not limited to the division. Production of drinking water encompasses two key aspects that must be investigated they are quality and quantity, however the close up analyses could only be successful completed for quality in the context of the quantity produced. Skills development planning within Scientific Services has always been based on the division’s feeder pipelines to be able to recruit from and retain scientific skills within the organisation. The division concentrates on Graduate, Bursar and Experiential Learner development ensuring a sustainable, trained and readily available pipeline of skills from which to recruit. Employees currently within the division both permanent and temporary form the type of scientific skills required for water quality monitoring and drinking water standard production and assurance. Employees have been placed within the functional scientific streams of the division and further by their levels of appointment and qualifications. The data analysis has also been done for the increasing of skills using the same framework. Age and gender was also included to show performance of the division in respect to transformation and equity. Equity in relation to growth is currently a global matter that is under scrutiny. The World Economic Forum has put equity in the spotlight to ensure countries look at their performance. The significance is that it has an impact on how the water resources in a country are distributed and managed. The Water Reforms in most developing countries have sparked large scale discussions around provisioning of water for all. Human Development and Water Resource Management are agendas that countries need to handle collectively with the ultimate outcome being achieving equity for all (UNDP, 2013). Rand Water’s Scientific Service skills data indicates that it has adequate scientific capacity to meet its present mandate of providing drinking water quality assurance for the organisation. There is some concern that the aging workforce is concentrated at management and specialists levels, therefore developing these skills for the next 5 to 10 years requires immediate attention. Transfer of skills and retention of skills requires careful strategic planning in order to attract a younger transformed workforce. The study shows that in as much as routine quality assurance is core, it is also equally critical to have employees who can troubleshoot within the context of the new environmental pressures and diverse operational conditions. The demand for quality drinking water over the last 110 years has increased throughout the country. The mandatory expansion of the organisation translates into sharing of human resources with other parts of the country to produce quality drinking water. Rand Water has been entrusted to take on the responsibility of other water utilities in the country and ensure that they reach the required standard for the production of quality drinking water. The full scope of the organisation’s mandate requires that it provide skills to handle the treatment of drinking water and wastewater in the near future. Although wastewater treatment is currently managed by the local municipalities, Rand Water will be having an active role to improve services. This would mean distributing the existing capacity within the organisation over a greater area of work along with a significant increase in the demand for scientific analyses of drinking water quality. The pace at which skills development takes place in Rand Water Scientific Services division shows that it will be able to meet the present needs. There are questions raised on the sustainability of the skills for the future. Maintaining and developing skills within the division is critical to be able to sustain the nature, structure and functioning of the division in its current form. The other factor that must also be maintained is the transformational equity demands of the country. The notion that there is a lack of experienced previously disadvantages scientists must be addressed directly to meet all the future demands of the sector, region and continent in a short space of time. / MT2017

M.Tech. (Biomedical Technology) / Drinking water in the greater Johannesburg area is produced by Rand Water and is transported to local Johannesburg Water where it is stored in reservoirs for distribution. At any point during the production, distribution and storage of the water, contamination with free-living amoebae, potentially containing amoeba resistant bacteria, may occur. Free-living amoebae are often resistant to the biocides used by water treatment industries and may thus be transmitted to public facilities, consumers’ homes and informal settlements through water distribution systems and during storage in small containers. The aim of our study was to analyse the water quality around Johannesburg with regard to free-living amoebae and amoeba resistant bacteria. A total of 182 tap and 5 storage tank water samples, collected from Hillbrow, Bertrams, Riverlea, Braamfischerville and Hospital Hill, were analysed for amoebae, indicator organisms, Legionellae, environmental mycobacteria, Shigella, Salmonella and Vibrio species using amoebal enrichment method. Direct microscopy indicated the presence of amoebae in 96.1% of samples. Acanthamoeba cysts were present in 69.0% of the samples. In 55.0% of these samples visibly active intracellular bacteria were observed within the sample suspensions. In the 46 samples analysed by polymerase chain reaction, the presence of Acanthamoeba species was confirmed in 65.2%, and the intracellular bacteria such as Legionella pneumophila and Mycobacterium avium was confirmed in 23.9% and 73.9% respectively. All samples indicated the presence of Shigella species while one sample contained Salmonella species on xylose lysine desoxycholate agar after amoebal enrichment processing. Vibrio species was not confirmed in the samples. Our results indicated a high risk of transmission of amoeba resistant bacteria through drinking water to people living in these areas.

There is no published data for the per capita consumption of water of individuals in South Africa. A daily rounded volume of 2 litres per person is usually taken as a working estimate from world wide data. As part of ongoing epidemiological studies into potential health effects of changes in the water supply to greater Town, water consumption patterns were ascertained. As health effects are often spatially ascribed to the place of residence of a person, it was necessary to ascertain how much water was drunk at home as well as away from home. Water consumed was divided into three classes: (i) water consumed from the tap, (ii) commercial beverages and (iii) water bound in food. A review of methods of conducting dietary surveys indicated that a 24-hour recall would be the most appropriate method. Two surveys on total dietary intake utilizing a 24-hour recall were carried out (n = 2 000 persons for each survey), one in winter and the ether in summer. The design of the survey involved a cluster sample of households that were representative of the socio-economic and demographic structure of greater Cape Town. Three pretested types questionnaires were administered by trained interviewers: (i) a placement questionnaire to describe the household composition, (ii) a recall questionnaire for individual adults and children and (iii) a recall questionnaire for babies. Particular attention was paid to the accurate ascertainment of the volumes of food and drink consumed as well as their preparation to facilitate accurate analysis. The water content of each food item was calculated by a computer program that utilized computerized food composition tables. The water consumption data was analyzed by sex, age, population group, income and the season of the year. Detailed graphs and tables are provided. Results were also standardized to the population of greater Cape Town. It was found that the difference in consumption between the White and "Coloured" population groups was greater than the difference between those people of high and low-income groups. The mean total water intake for Whites was 2.19 litres per day, while for "Coloureds" it was 1.26 litres per day. There is no obvious bias to account for this difference. The figures for protein consumed by the two groups, which was used as a control, are consistent with values reported in the literature. Summer consumption was higher than that during winter. The ratio of tap water consumed at home to total liquid consumed was approximately 0.5.

In September 2000 water fluoridation became mandatory in South Africa. Since then water service providers like Umgeni Water (UW), a bulk water supply authority in the KwaZulu-Natal (KZN) province of South Africa began the process of implementing the legislation. This study was undertaken to establish the spatio-temporal variations of fluoride concentrations in surface and ground waters within the Umgeni Operational Area, to establish whether these waters would require fluoridation or defluoridation to meet a fluoride concentration of 0.70 mglf, and to assess the potential impacts of water fluoridation. Baseline fluoride concentrations of surface and ground water: It was concluded that the fluoride concentration of all sample types (rivers, dams, water works raw and final waters, wastewater influent and effluents, and boreholes), except pollution point sources, is less than O.S; mglR, 50 percent of the time. Some rivers (Mshazi, KwaNyuswa, KwaNgcolosi,·Mshwati and the MgoShongweni) exhibited high fluoride concentrations, while someboreholes also exhibited high fluoride concentrations. Temporal Variations and Seasonality: There are seasonal variations in the fluoride concentrations for surface waters, with higher fluoride concentrations in winter than in summer (64 out of 125 occasions). This low fluoride concentration in summer can be attributed to the dilution effects caused by rainfall runoff. Identification of "Hot Spots": "Hot Spots", sites where the fluoride concentration exceeds 1 mglR are present within the study area, for surface and borehole water. For surface water, the MgoShongweni exhibited fluoride concentrations in excess of 1mglRat least 75% of the time. The KwaNgcolosi and Mshwati exhibited fluoride concentrations In excess of 1mglR at least 25% of the time, while the Mshazi and the KwaNyuswa exhibited fluoride concentrations in excess of 1mglR only 5% of the time. The storm water discharge below AECI had high fluoride concentrations in excess of 1mg/R at least 20% of the time and the concentrations exceeded the fluoride concentration for seawater (1.4 mglf) at least 5% of the time. Of the 286 boreholes sampled, 17 boreholes (6% of all boreholes sampled) had fluoride levels in excess oft mglf . The impacts of long term consumption of water from these boreholes could range from slight mottling of the dental enamel in sensitive individuals (boretioles JD26, C29, H19, CB7, 112/1, 69/5, Thembeni 108 and EC (Thembeni 105, Keats Drift boreholes 1 and 2). Spatial patterns and possible sources of high fluoride concentrations: With respect to spatial patterns, relatively high concentrations of fluoride (300 IJglR to 1000 IJglR) can be found in surface water in the Msunduzi river, the Mgeni river downstream of the Msunduzi confluence and along the coastal belt. No spatial patterns are evident with respect to borehole water. For surface water, high fluoride concentrations in the Mshazi, KwaNyuswa and the KwaNgcolosi streams (inflows to the Inanda dam) appear to be associated with the catchment geology. The ~igh fluoride concentrations in Mshwati and the MgoShongweni are most likely as a result of industrial activities in the respective catchments. For borehole water, high fluoride concentrations may be attributed to catchment geology. Additional fluoride dosaqe ' at water treatment works: Since the fluoride concentrations at the water works were low (mean ranging between 0.5 mglf to 0.38 mglf) , fluoride would need to be added to meet the fluoride standard of 0.7 mgl£ . For most of the water works, the additional fluoride (sodium fluoride) requirement to meet the fluoride standard of 0.7 mgl£, ranged from 1.201 kglMRto 1.555 kg/MR. For the water works, Imfume and Umzinto, the additional fluoride , requirement is 0.768 kg/MR and 0.109 kg/MR respectively. In final water, the fluctuations in fluoride concentrations observed would translate to continuous testing being required to maintain optimal dosing of fluoride. Comparison of influent and effluent fluoride concentrations at wastewater works: There was no evidence of fluoride removal at the Mpophomeni Wastewater Works . There was evidence of 22.4% fluoride removal at the DarvHI Wastewater Works possibly due to the activated sludge treatment process at the wastewater works. Future fluoride levels in surface water that will receive return flows: Once water fluoridation is implemented, the Darvill Wastewater Works would receive fluoridated return flows, and discharge its fluoride rich effluent into the Msunduzi river. The average monthly fluoride road discharged from Darvill Wastewater Works would increase from 0.23 tons to 1.46 tons, an additional 1.23 tons per month on the aquatic environment of the Msunduzi river. The sludge fluoride load, disposed to land, could increase from 4 056 tons/month to 27 863 tons/month, which implies an increase in the fluoride runoff potential from the sludge-lands to the Msunduzi river. Number of people in sensitive groups that could be affected by water fluoridation: A significant number of people in KZN could be sensitive to water fluoridation. This has been estimated to be at least one third of KZN's population that are HIV infected. Recommendations were made and the most important ones are as follows: In the light of the large number of people, one-third the population of KZN, that is HIV positive and therefore could be sensitive to fluoridated water, it is recommended that the South African legislation mandating water fluoridation be withdrawn. Examination of the most recent literature indicated a significant lack of confidence in the best available studies that researched the safety and efficacy of water fluoridation. In the light of this it is recommended that the South African Department of Health re-examine and withdraw its legislation that mandates water fluoridation. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2002.

Thesis submitted in partial fulfilment of the requirements for the degree of MAGISTER TECHNOLOGIAE: CHEMICAL ENGINEERING in the FACULTY OF ENGINEERING at the CAPE PENINSULA UNIVERSITY OF TECHNOLOGY 2013 / This study focused on quantifying two types of internationally regulated contaminants found in drinking water: 1) Trihalomethanes (THMs) and 2) Perfluorinated compounds (PFCs). The first contaminants monitored were THMs, classified as a group of chemicals that are formed along with others during the disinfection of water using liquid chlorine, chlorine dioxide or chlorine gas. Hence, the resulting compounds are called disinfection by-products (DBPs). The disinfectant reacts with natural organic matter in water to form common THMs, which include chloroform (CHCl3 or CF), bromodichloromethane (CHCl2Br or BDCM), dibromochloromethane (CHClBr2 or DBCM) and bromoform (CHBr3 or BF), with chloroform being the most common in chlorinated water systems. The current study has focused on THMs for two primary reasons: 1) THMs have raised significant concern as a result of evidence that associate their presence in drinking water with potential adverse human health effects, including cancer and 2) the levels of THMs in drinking water post-treatment is not monitored regularly in South Africa and thus far, there is inadequate and limited information about their concentration levels for drinking water treatment plants (DWTPs) and distribution stations (DWDSs) of the Western Cape, South Africa before, distribution to various suburbs, including townships. THMs normally occur at higher levels than any other known DBPs and their presence in treated water is a representative of the occurrence of many other DBPs. THMs were quantified in chlorinated drinking water obtained from seven (7) DWTPs, namely; Atlantis, Blackheath, Faure, Brooklands, Steenbras, Voelvlei and Wemmershoek, and one DWDS in Plattekloof. This included determining THMs concentration in tap water collected from various suburbs including townships, to assist local authorities in obtaining information on their concentration and whether or not the presence of residual chlorine and organic matter on post-treatment results has increased THMs at the point of use. THM analysis was performed using liquid-liquid extraction/gas chromatography with electron capture detector (LLE-GC-ECD) analytical process according to the EPA method 501.2, which was used with minor modifications. The instrument operational conditions were as follows: Column → DB5-26, 30 mm, 0.53 mm, 1.0 μm df HP-1 (Agilent Technologies, USA); Carrier gas → Helium at a constant inlet pressure of 15 kPa; Make-up gas → 99.9% Nitrogen gas at 60 L/min; Injector temperature → 40°C; Oven temperature → 270°C and Detector temperature → 300°C. Since natural organic matter (NOM) in raw water is a precursor for THM formation, NOM analysis was performed as total organic carbon (TOC) using Spectroquant TOC test kits. Other drinking water quality parameters analysed were pH, residual free chlorine, conductivity and total dissolved solids (TDS). The average Total THM concentrations detected from seven of the DWTPs, including the DWDS, ranged from 26.52 μg/L (for Plattekloof) to 32.82 μg/L (for Brooklands), with the observed concentrations being comparable. The average chloroform concentrations were the highest in all the water samples, ranging from 11.74 μg/L (for Plattekloof) to 22.29 μg/L (for Voelvlei), while DBCM had the lowest concentration. The only DWTP that was not comparable with the seven DWTPs was Atlantis, with the highest average TTHM concentration of 83.48 μg/L and a chloroform concentration of 46.06 μg/L. From the tap water samples collected from 14 Western Cape suburbs, the average TTHM concentrations ranged from 5.30 ug/L (for Mandalay) to 13.12 μg/L (for Browns Farm, Philippi), and all these concentrations were lower than the TTHM concentrations detected in the water samples from the DWTP. Overall, the average total THM and individual THM species concentrations were below the recommended SANS 241:2011 and WHO drinking water guideline limits. This included the observed pH (6.39 to 7.73), residual free chlorine (0.22 to 1.06 mg/L), conductivity (121 to 444 μS/cm), TDS (93.93 to 344.35 mg/L) and TOC (0.38 to 1.20 mg/L). All these water quality parameters were within the specification limits stipulated in SANS 241. However, the average residual free chlorine concentration for Atlantis was very low (0.06 mg/L), which was below the WHO minimum residual free chlorine concentration guideline value of 0.2 mg/L for a distribution network – an indication that suggested the need for a re-chlorination station prior to distribution to households. Low chlorine content might result in the formation of unwanted biofilms in the distribution network, thus reducing the organoleptic properties of the water. Additionally, there was no direct link between several water quality parameters quantified (i.e. pH, TOC and water temperature) to TTHM formation. However, a high chlorine dose was observed to result directly in a higher concentration of chloroform in treated water prior to distribution. The second contaminants monitored were Perfluorinated compounds (PFCs), which are non-biodegradable, persistent and toxic organic chemicals known for their ability to contaminate environmental matrices, including drinking water sources. In recent years, many researchers considered it essential to identify and quantify PFC levels in drinking water worldwide with the main focus being on the two most abundant PFCs; namely Perfluorooctanoic acid (PFOA) and Perfluorooctane sulfonate (PFOS). Their toxic effects to human health, plants and wildlife were also evaluated, classifying them as possible carcinogens. We know from the literature reviewed that, although the presence of PFCs in drinking water has been documented worldwide, there is limited information about their presence specifically in South African drinking water sources, even about less studied PFCs such as Perfluoroheptanoic acid (PFHpA), Perfluorododecanoic acid (PFDoA), Perfluorononanoic acid (PFNA), Perfluoroundecanoic acid (PFUA), Perfluorodecanoic acid (PFDeA) and the well-known PFOA including PFOS. Although several other PFCs have been detected in water sources and reported in various studies, the USEPA only issued drinking water guideline limits for Perfluorooctanoic acid (PFOA) and Perfluorooctane sulfonate (PFOS) of 400 ng/L and 200 ng/L, respectively, with no mention of the other PFCs. However, these PFCs have similar properties to those of PFOA and PFOS as they have been shown to impose similar detrimental health effects on human health. This study thus focused on the detection of PFCs in both raw and treated drinking water in the Western Cape DWTPs such as Atlantis, Blackheath, Faure, Brooklands, Steenbras, Voelvlei and Wemmershoek, and one DWDS in Plattekloof. Water samples (raw and treated water) used in this study for PFC analysis were collected in 2L polypropylene screw capped bottles. PFC analysis was performed in four sample batches for each location collected through the period of October to December 2012 (summer). PFCs were analysed in accordance with a modified EPA method 537, which entails solid phase extraction (SPE) followed by analysis using a liquid chromatography/tandem mass spectrometer (LC/MS/MS). The slight modification was with the water sample volume used for extraction, which was increased from 250 mL to 500 mL. The instrument used was an HPLC - Ultimate 3000 Dionex HPLC system and MS model - Amazon SL Ion Trap, with the following MS/MS operational conditions and Ion mode: MS Interface → ESI; Dry temp → 350C; Nebulizing pressure → 60 psi; Dry gas flow → 10 L/min; Ionisation mode → negative; capillary voltage → +4500V; End plate offset → −500V while the separation column was a Waters Sunfire C18, 5 μm, 4.6 × 150 mm column (Supplier: Waters, Dublin, Ireland) with an operational temperature of 30C. From the results obtained in this study, seven different PFCs (i.e. PFHpA, PFDoA, PFNA, PFUA, PFDeA, PFOA and PFOS), were detected in raw and treated water with PFOA and PFOS being the least detected PFCs as they were detected only in raw water (PFOA) from Faure, as well as raw and treated water (PFOS) from Brooklands. The highest concentration observed in treated water was for PFHpA, which was quantified at a maximum average concentration of 43.80 ng/L (Plattekloof). The maximum average concentrations of other PFCs detected were as follows: PFDoA - 4.415 ng/L for Faure raw water; PFNA - 2.922 ng/L for Plattekloof outlet; PFUA - 7.965 ng/L for Brooklands treated water and PFDeA - 2.744 ng/L for Faure raw water. Another observation from the results was that the concentration of the majority of the PFCs detected in treated water was higher than that quantified in raw water, suggesting possible contamination by materials used during water treatment. In conclusion, THMs detected in treated water from various DWTPs and one DWDS in the Western Cape met the required local and international drinking water quality guidelines, while the presence of PFOS, PFOA, PFHpA, PFDoA, PFNA, PFUA and PFDeA in treated water requires that local water professionals continue to monitor their presence to ensure that measures for their reduction are in place. Furthermore, the National standards (SANS 241) for municipal drinking water guidelines must be updated to include the monitoring of PFCs, including the lesser known and less studied PFCs such as PFHpA, PFDoA, PFNA, PFUA and PFDeA.

Thesis (MBA)--Stellenbosch University, 2012. / This study gives an overview of the sustainability of potable water supply in rural areas of South Africa in general and four rural areas of uMhlathuze in particular. Three key challenges in achieving sustainable rural water supply are discussed in more detail and later on used to evaluate the inadequacy of financial revenues to cover the full operation, maintenance and replacement of infrastructure. This research study analysed the factors pertaining to the tariff structure used in maintaining and sustaining rendered service. It analysed the current tariff structure that includes the poorest and most marginalised in line with revenue needed to cover recurrence costs. It was the purpose of this study to examine the adequacy of the management system used to sustain the supply of potable water in rural areas, taking into cognisance the costs of rendering the account and of illegal connections combined with high water losses. The study also attempted to link these points to the challenges faced by the rural areas. The sustainability of rural water supply was analysed, based on financial factors, affordability and on the willingness to pay for the service. The researcher first compared the water billing (levies) and payment patterns of each customer in rural areas of uMhlathuze Municipality with others; and secondly, compared the primary data against theory and the literature. Differences and similarities between the collected data and theory were at the core of the analysis The research results determined that the municipality has the capacity to maintain and sustain the potable water supply network in these areas, while dealing with management questions and recommending to management what is needed to ensure that the water reticulation system is run on a sustainable basis. Sustainability of rural water supply seems to be dependent on factors like policy, legal framework and economic factors such as an ability to meet the costs and willingness to pay for rendered service.

MSc (Microbiology) / Department of Microbiology / See the attached abstract below

In 1994, South Africa adopted intergovernmental relations (IGR) to facilitate service delivery. Sections 40-41 of the Constitution of the Republic of South Africa, 1996, link service delivery with normative aspects of IGR, which include cooperation, transparency, accountability, mutual support, and coherence. A coherent implementation of IGR was subsequently emphasised by the Intergovernmental Relations Framework Act 13 of 2005. However, South Africa continues to experience service delivery challenges, especially in marginalised and poor communities in the current and former informal settlements. The selected Diepsloot was established as an informal settlement in 1995 and has been under an in situ upgrade programme. The study’s focus was on the provision of potable water in the City of Johannesburg with specific reference to Diepsloot. A case study approach was used to assess lived experiences among the actors within the intergovernmental context of cooperative government. A qualitative methodology was utilised to source data about intergovernmental interactions among actors from the public institutions by means of semi-structured interviews and documentary analysis. Lastly, a focus group was utilised for members of the ward committees in Diepsloot. The study concluded that IGR system is not used adequately to support Diepsloot to access potable water in accordance with an established standard. In essence, the IGR system lacks an integrated approach to reverse a legacy of informality. To facilitate an IGR improvement, the study’s recommendations were three-fold: (i) provision of integrated support to the City of Johannesburg for Diepsloot despite erroneous assumption that metropolitan municipalities are self-sufficient, (ii) standardisation of potable water provision in Diepsloot by means of integrating IGR institutional responses, and (iii) institutionalisation of IGR engagements with other cities. To this end, the study proposed a model of integrated intergovernmental support to improve potable water provision and, by extension, other related services in Diepsloot. / Public Administration / D.P.L. (Public Administration)

MESHWR / Department of Hydrology and Water Resources / This research involves the investigation of the interaction of different sized Nano Gold particles released into municipal drinking water and municipal waste water. Waste water was collected from Malamulele waste water treatment plant and the municipal water was collected at Mintek in Johannesburg, Randburg, South Africa. The waste water was analysed using ICP-MS to detect the metals and anions in it. The results showed the abundance of Sulphur (464 ppm), Calcium (28 ppm), Chloride (27.8 ppm), Iron (20 ppm), Magnesium (8.2 ppm), silicon (6.192 ppm) in descending order and other trace elements, including gold, that were immeasurable (<0.1). The simulated situation was created by adding 20nm gold and 40nm gold nanoparticles into municipal drinking water and waste water and kept at different environmental conditions (light, light and agitation, dark, dark and agitation) under aerobic and anaerobic conditions over a period of two months. Physico-chemical properties (pH and chemical oxygen demand) of the solutions were checked once in a month. The pH fluctuated between the acceptable ranges (5.5 – 9.5) for the two month period. Both municipal water and waste water, with and without gold nanoparticles, under aerobic condition showed an increase in chemical oxygen demand. The gold content in waste water under anaerobic condition showed an increase while under aerobic condition the decline in gold content was evident. The zeta potential of gold nanoparticles in waste water in light and agitation showed (-30 mV) while waste water on other environmental condition (light, dark and dark with agitation) presenting unstable (-18 mV) charge, but the charge shifted positively on the second month rendering them also unstable. Dynamic light scattering and TEM were used to check any possible aggregation or agglomeration of nanoparticles in the waste water. There were some few discrepancies where TEM and DLS contradict, but overall there was no significant probability of any aggregation of gold nanoparticles. The EDX was used to confirm the presence of Au0 in the waste water (with added gold nanoparticles). The research did show that the gold nanoparticles would exist as Au0 in the waste water and thus the discharge of Au-NPs to the sewer system is not recommended, but rather recycle them.