Glucansucrases são enzimas que atuam em reações de síntese de polissacarídeos e oligossacarídeos. Para que esses biocatalisadores sejam aplicados em escala industrial, é desejável ótimas estabilidades térmica e operacional, o que pode ser alcançado com a imobilização de enzimas. Como alternativa aos suportes sólidos amplamente estudados, está a quitosana, polímero que não apresenta toxicidade e possui alta biocompatibilidade e alta afinidade com proteínas. Outra possibilidade promissora na imobilização de enzimas, é a síntese dos agregados enzimáticos entrecruzados (CLEAs), os quais apresentam alta atividade catalítica e alta estabilidade. Contudo, uma peculiaridade das glucansucrases quando produzidas em meio contendo sacarose é a camada de polímero que as envolve, e que bloqueia o acesso aos grupos reativos na superfície da proteína. No caso da expressão heteróloga das glucansucrases em Escherichia coli essa dificuldade pode ser contornada. Além disso, o uso da mutagênese sítio-dirigida pode proporcionar modificações de aminoácidos na superfície da enzima, tais como os resíduos Lys, Cys, His, com o intuito de que melhorias na imobilização sejam alcançadas. Sendo assim, na primeira etapa desse trabalho, uma extensa discussão é apresentada em relação às metodologias de imobilização de dextransucrase encontradas na literatura. A seguir, estudos referentes à imobilização da dextransucrase de Leuconostoc mesenteroides B-512 F em esferas de quitosana ativadas com glutaraldeído foram realizados. Esse imobilizado apresentou alta atividade catalítica (197 U/g) quando utilizada a carga de proteína de 400 mg/g de suporte. Além disso, observou-se que a imobilização covalente e os açúcares maltose e glicose promoveram proteção à enzima em temperaturas de 40 ºC e 50 ºC. Na etapa seguinte, a produção e a caracterização de CLEAs de dextransucrase de L. mesenteroides B-512 F foram investigados. Demonstrou-se que o tratamento com a dextranase foi essencial para a imobilização da glucansucrase e que o isopropanol foi o melhor agente precipitante. Os CLEAs apresentaram pH e temperatura ótimos de 3,0 e 60 ºC, respectivamente, enquanto que a dextransucrase imobilizada nas esferas de quitosana funcionalizada com glutaraldeído apresentaram os valores de 4,5 e 20 ºC. Ambas formas imobilizadas apresentaram boa estabilidade operacional na síntese de oligossacarídeos uma vez que após 10 ciclos, 40 % de atividade residual foi observada. Por fim, estão apresentados estudos sobre a modelagem das estruturas tridimensionais e a mutagênese sítio-dirigida das glucansucrases DSR-S vardel Δ4N and ASR C-APY del. Os modelos preditos demonstraram boa qualidade e a mutagênese sítio-dirigida não promoveu perdas significativas na atividade enzimática dos mutantes. Somente o mutante DSR_S326C mostrouse inativo. Os resultados obtidos sugerem que a imobilização da dextransucrase foi satisfatória e que cada técnica possibilita diferentes características ao imobilizado. Além disso, os imobilizados foram adequados para síntese de dextrana e oligossacarídeos. / Glucansucrases are enzymes that catalyze the synthesis of polysaccharides and oligosaccharides. In order to assure continuous processing and reuse of the biocatalyst in industrial applications, enzyme immobilization techniques are required to promote good thermal and operational stabilities. Among the several solid supports for enzyme immobilization, chitosan shows interesting properties because it is non-toxic, it is biocompatible, and it has high protein affinity. Other possibility is the production of cross-linked enzyme aggregates (CLEAs), which presents high catalytic activity and good stability. However, glucansucrases have a particularity when produced in sucrose medium, since a polymer layer surrounds the protein, blocking the access to reactive groups on the enzyme surface. To overcome this problem, it is possible to make the heterologous production of glucansucrases in Escherichia coli. Likewise, the site-directed mutagenesis may promote changes in the amino acids located on the surface to improve immobilization parameters. Therefore, this work aimed to discuss the several techniques applied for dextransucrase immobilization, and to design new immobilized biocatalysts. In a first step, it is presented a review about the distinct immobilization methodologies for dextransucrase. In a second study, an investigation about dextransucrase from Leuconostoc mesenteroides B-512 F immobilized on glutaraldehyde-activated chitosan particles was carried out. The novel immobilized biocatalyst showed 197 U/g (400 mg/g dried support) of catalytic activity. The covalent immobilization promoted protection against enzyme damages at 40 ºC and 50 ºC, whereas maltose and glucose acted as stabilizers. Furthermore, it was studied the production and characterization of CLEAs dextransucrase from L. mesenteroides B-512 F. It was demonstrated that dextranase treatment was crucial for immobilization. Isopropanol was chosen as the best precipitant agent. CLEAs presented optimal pH and temperature of 3.0 and 60 ºC, respectively, whereas it was found values of 4.5 e 20 ºC for dextransucrase immobilized on glutaraldehyde-activated chitosan particles. Both immobilized biocatalysts showed good operational stability in the oligosaccharides synthesis, exhibiting 40 % of residual activity after 10 cycles. Finally, the study concerning the homology modeling and site-directed mutagenesis of glucansucrases DSR-S vardel Δ4N and ASR C-APY del is presented. The predicted models showed good quality and it has been demonstrated that the site-directed mutagenesis did not promote significant losses in the variant enzyme activities. Only one mutant (DSR_S326C) had shown no dextransucrase activity. The results obtained in this work suggest that the immobilization of dextransucrase was satisfactory, also showing that each technique promotes different characteristics to the immobilized biocatalyst. Besides, these immobilized enzymes were feasible for the synthesis of dextran and oligosaccharides.

This research introduced a geological perspective into an archaeological setting, the Ness of Brodgar, a Middle to Late Neolithic site in the West Mainland of Orkney. Discovered in 2003, the site is located on an isthmus of land between two lochs and is equidistant between the Ring of Brodgar and the Stones of Stenness stone circles. The site consists of more than two dozen drystone buildings encircled by a massive wall. To date, ten per cent of the site has been excavated. This research established a systematic framework of protocols and procedures for the recovery, macro geological assessment and identification of the rock and mineral species in the non-tool, non-structural rocks, termed Foreign Stone for this research. Once identified, the frequency and distribution of the rock in the Foreign Stone finds were calculated, providing a geological basis for understanding any patterns of rock choice across the site. Integrated into a more conventional archaeological study of the Worked Stone artefacts was a systematic macro petrological analysis of these finds. Additionally, selected segments of the interior drystone walling were assessed to identify the rock in the various members. These petrologic analyses combined to provide insight into the range of rocks transported to and utilized within the site. Comparative analysis of the data from all three aspects, Foreign Stone, Worked stone and walling, was undertaken to identify trends in frequencies and patterns of use of the various rock species. Archival and more current information on the petrologic resources available within Orkney were synthesized in a gazetteer identifying the location(s) of rock outcrops and deposits. This permitted the source location(s) of many of the rocks from the Ness of Brodgar to be identified.

O presente trabalho trata das relações estabelecidas entre a preservação do patrimônio edificado e o planejamento e a gestão urbana do sítio histórico da Cidade de Sobral, tombado pelo Instituto do Patrimônio Histórico e Artístico Nacional - IPHAN em 1999. Com ênfase para as novas abordagens do tombamento federal de áreas urbanas no Brasil e em outros países, em seus aspectos políticos, gerenciais e transformadores, a experiência de preservação urbana em Sobral é analisada sob a ótica dos conceitos de cidade-monumento, cidade-documento, cidade-empreendimento e cidade-instrumento, esta a hipótese principal da tese. / This work deals with relations established between the preservation of the built cultural heritage and urban planning and management of the historic site of Sobral, protected as nacional monument by Instituto do Patrimônio Histórico e Artístico Nacional - IPHAN since 1999. Emphasizing new approaches of the federal listing of urban areas in Brazil and other coutries, in its political, administrative and changing aspects, Sobral urban preservation experience is analyzed trough the concepts of cidade-monumento, cidade-documento, cidade-empreendimento e cidade-instrumento, this the main hipothesis of the work.

Chan Kam Leung. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1999. / Includes bibliographical references (leaves 175-182). / Abstract also in Chinese. / Acknowledgements --- p.i / Abstract --- p.ii / Abbreviations --- p.v / Table of Contents --- p.vii / Chapter Chapter 1 --- General Introduction / Chapter 1.1 --- Drosophila as a model for studying development --- p.1 / Chapter 1.2 --- The formation of the body axis in Drosophila --- p.2 / Chapter 1.3 --- The maternal genes are essential for development --- p.9 / Chapter 1.4 --- Maternal gene bicoid is essential for formation of the anterior structures in the embryo --- p.11 / Chapter 1.5 --- The formation of the biocid protein gradient from anterior pole to posterior pole of the embryo --- p.13 / Chapter 1.6 --- The bed protein gradient controls the downstream zygotic target genes in a concentration-dependent manner --- p.15 / Chapter 1.7 --- The formation of the bed protein gradient in embryo --- p.17 / Chapter 1.8 --- Components required for bcd mRNA localization at anterior pole of oocyte --- p.21 / Chapter 1.8.1 --- Cis-acting elements --- p.21 / Chapter 1.8.2 --- Trans-acting elements --- p.21 / Chapter 1.9 --- The properties of exuperantia protein --- p.25 / Chapter 1.9.1 --- The function of exu protein --- p.25 / Chapter 1.9.2 --- Exuperantia is a phosphoprotein --- p.26 / Chapter 1.9.3 --- Phosphorylation pattern of exuperantia protein is stage-specific --- p.28 / Chapter 1.9.4 --- Reversible phosphorylation is one of the major mechanisms to control protein activity in all eukaryotic cells --- p.29 / Chapter 1.9.5 --- The relationship between the exu protein phosphorylation and the bcd mRNA localization --- p.30 / Chapter 1.10 --- Aim of project --- p.31 / Chapter CHAPTER 2 --- Preparation of the exuperantia genomic DNA and complement DNA (cDNA) mutant Constructs / Chapter 2.1 --- Introduction --- p.33 / Chapter 2.2 --- Materials and methods --- p.35 / Chapter 2.2.1 --- DNA preparation methods --- p.35 / Chapter 2.2.1.1 --- Preparation of double-stranded DNA by polyethylene glycol6000 --- p.35 / Chapter 2.2.1.2 --- Preparation of M13mp8 single-stranded DNA --- p.37 / Chapter 2.2.1.3 --- "Preparation of double-stranded DNA by Biol prep (Modified from Maniatis et al.,1989)" --- p.38 / Chapter 2.2.2 --- "Preparation of DH5α，JM109, TG1 competent cells" --- p.39 / Chapter 2.2.3 --- Bacteria transformation --- p.40 / Chapter 2.2.4 --- Restriction enzyme digestion --- p.40 / Chapter 2.2.5 --- Phenol/chloroform extraction --- p.41 / Chapter 2.2.6 --- Purification of DNA fragment by electro-elution --- p.42 / Chapter 2.2.7 --- DNA ligation --- p.43 / Chapter 2.2.8 --- DNA dephosphorylation --- p.43 / Chapter 2.2.9 --- In vitro site-directed mutagenesis --- p.44 / Chapter 2.2.9.1 --- The Sculptor´ёØ in vitro mutagenesis --- p.44 / Chapter 2.2.9.2 --- The GeneEditor´ёØ in vitro site-directed mutagenesis --- p.47 / Chapter 2.2.10 --- The double-stranded or single-stranded DNA sequencing by T7 DNA polymerase sequencing system --- p.50 / Chapter 2.2.11 --- Denatured polyacrylamide gel electorphoresis --- p.51 / Chapter 2.2.11 --- Nucleotide sequence of the sequencing primers and the mutageneic oligonucleotides --- p.54 / Chapter 2.3 --- Results --- p.55 / Chapter 2.3.1 --- Design exuperantia mutant constructs --- p.55 / Chapter 2.3.1.1 --- Comparison of exu protein amino acids sequence with different Drosophila species --- p.56 / Chapter 2.3.2 --- The exu genomic mutant constructs --- p.63 / Chapter 2.3.3 --- The exu cDNA mutant constructs --- p.63 / Chapter 2.4 --- Discussion --- p.76 / Chapter CHAPTER 3 --- Epitope tagging of exuperantia protein with c-myc eptiope / Chapter 3.1 --- Introduction --- p.79 / Chapter 3.2 --- Materials and methods --- p.84 / Chapter 3.2.1 --- Preparation of the c-myc eptiope DNA fragment --- p.84 / Chapter 3.2.2 --- End-filling of 5'overhang DNA fragment by Klenow fragment --- p.86 / Chapter 3.2.3 --- In vitro translation of protein by TNT® Quick coupled transcription and translation system --- p.86 / Chapter 3.2.4 --- Immunoprecipitation of recombinant exu protein --- p.87 / Chapter 3.2.5 --- Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) --- p.88 / Chapter 3.2.5.1 --- SDS-PAGE preparation --- p.88 / Chapter 3.2.5.2 --- SDS-PAGE electrophoresis --- p.90 / Chapter 3.2.6 --- Western blot analysis --- p.90 / Chapter 3.2.6.1 --- Transfer the protein to a nitro-cellulose membrane by semi-dried blotting --- p.90 / Chapter 3.2.6.2 --- Western blot blocking and antibody recognition --- p.91 / Chapter 3.3 --- Results --- p.92 / Chapter 3.3.1 --- Construction of the plasmid containing exu cDNA tagging with a c-myc epitope --- p.92 / Chapter 3.3.2 --- In vitro translation of c-myc epitope tagged exu protein --- p.102 / Chapter 3.3.3 --- Immunoprecipitation of c-myc labeled exu protein by a polyclonal rabbit anti-exu antibody and monoclonal mouse anti-myc antibody --- p.104 / Chapter 3.4 --- Discussion --- p.109 / Chapter CHAPTER 4 --- In vitro phosphorylation of exuperantia Protein / Chapter 4.1 --- Introduction --- p.111 / Chapter 4.2 --- Materials and methods --- p.113 / Chapter 4.2.1 --- Exogenous kinase phsophorylation reactions --- p.113 / Chapter 4.2.2 --- Separation of the phosphorylated exu protein variants by SDS- PAGE --- p.114 / Chapter 4.3 --- Results --- p.115 / Chapter 4.3.1 --- Western blot analysis of in vitro translated exu protein variants --- p.115 / Chapter 4.3.2 --- Phosphorylation of in vitro translated exu protein variants by exogenous cAMP-dependent protein kinase --- p.118 / Chapter 4.3.3 --- Phosphorylation of in vitro translated exu protein variants by exogenous cGMP-dependent protein kinase --- p.123 / Chapter 4.3.4 --- Phosphorylation of in vitro translated exu protein variants by exogenous protein kinase C --- p.128 / Chapter 4.4 --- Discussion --- p.133 / Chapter CHAPTER 5 --- Introduction of the exuperantia genomic constrcuts into the germline of Drosophila by P element-mediated transformation / Chapter 5.1 --- Introduction --- p.136 / Chapter 5.2 --- Materials and methods --- p.138 / Chapter 5.2.1 --- Construction of a genomic construct for production of transgenic flies --- p.138 / Chapter 5.2.2 --- Preparation of double-stranded DNA by ultra-centrifugation --- p.142 / Chapter 5.2.3 --- P-element mediated transformation --- p.143 / Chapter 5.2.3.1 --- Eggs collection --- p.143 / Chapter 5.2.3.2 --- Dechorionating the eggs --- p.143 / Chapter 5.2.3.3 --- Orientating the eggs --- p.144 / Chapter 5.2.3.4 --- Microinjection --- p.145 / Chapter 5.2.4 --- Collecting virgin female Drosophila --- p.146 / Chapter 5.2.5 --- Setup a crossing experiment --- p.146 / Chapter 5.2.6 --- Preparation of total ovaries and testes extracts exu protein from Female and male Drosophila --- p.147 / Chapter 5.2.7 --- Immunohistochemical distribution of exuperantia protein --- p.147 / Chapter 5.3 --- Results --- p.150 / Chapter 5.3.1 --- Insertion of the mutated exu fragments into the Drosophila Transformation vector (pCaSpeR) --- p.150 / Chapter 5.3.2 --- Introduction of the mutated exu gene into the genome of Drosophila by P-element mediated transformation --- p.153 / Chapter 5.3.3 --- Western blot analysis of the exu protein in the exu (ES2.1) transgenic fly --- p.160 / Chapter 5.3.4 --- Immunohistochemical distribution of exu protein in exuES21 mutants --- p.162 / Chapter 5.3.5 --- Rescue test of exuES2.1 trangenic flies --- p.165 / Chapter 5.4 --- Discussion --- p.168 / Chapter CHAPTER 6 --- General Discussion --- p.171 / References --- p.173 / Chapter Appendix I: --- List of reagents --- p.183 / Chapter Appendix II: --- Publication --- p.187

Following a call from Linton (2010) to think more relationally about water this thesis seeks to explore the infolding and unfolding relations that take-form between bodies around particular characteristics of freshwater. There is a tradition of exploration regarding the sustainability, quality, monitoring and management of water when we encounter research on human associations with fluvial hydrology, and whilst this work is important, this project looks to enrol more nascent and contemporary geographical themes to broaden our understanding of encounters with freshwater landscapes, and take a more relational approach to fluvial geographies. These works then shall address a gap in the geographical literature and describe the personal, pre-personal and affective worlds that emerge when bodies become down by the river. Whilst this is not specifically a walking project, walking the course of the River Severn serves as a trajectory along which processual ideas of bodies on the move shall be mobilised. A series of creatively written segues will link together a sequence of theoretical and conceptually driven site ontologies (Marston et al 2005; Woodward et al 2010) and relations associated with the Severn and freshwater more broadly. The flow and form of the thesis will reflect the multivariant characteristics of water and its varying speeds and slownesses. The chapters will step into puddles, mooch about in a ships graveyard, rethink the source of a river, paddle a coracle and set the scene for how an ontological, relational approach to fluvial landscapes can contribute to geographical thinking. The works will focus on human-nonhuman relations, vibrant materialities and elemental mobilities, in so doing enable further understanding of how we can apprehend sites as moments of coherence in a turbulent world, and contribute to broadening our scope of knowledge of the more-than-human.

"Nowadays, many companies in the Architecture/Engineering/Construction (AEC) industry are using Building Information Modeling (BIM) in achieving a faster, sustainable and more economic project. Among the new developed concepts and BIM applications, two of the concepts most frequently used with the support of BIM technology in the planning, organization and scheduling of projects are 4D and 5D in which a 3D model is tied to its time execution (4D) at any point in time and its corresponding cost (5D). However, most of these applications concentrate on modeling the building but it does not include a corresponding modeling of the site in which the building is located. To date, there are few studies and systematic implementation of the site and the building integrated into one BIM model. This site-building integrated model can also be conceptualized as ¡°6D BIM¡± model. The benefit of integrating the site and building together into one model is that the building is no longer treated in isolation of its surround site but incorporates extremely helpful short-term and long-term information for the owner, designer, and builder regarding site topography, landscaping, access roads, ground conditions and the location of site utilities. Major existing research and technology issues that are preventing this site-building integration deal with functionality and interoperability of the BIM software, different orientation and coordination of building model and site model. The objectives of this thesis are to explore current organizational and technological issues preventing this integration, to investigate a feasible method to create a site-linked BIM model, and to discuss the benefits and limitations of bringing BIM concept to the site conditions. The research has been conducted by an extensive review on the literature related to the topic of interest published primarily by AEC. A review on current applications of Geographic Information Systems (GIS) has also been included because of the wider context provided by this technology to the specific topic of this research. Related BIM software developed by three different vendors ¡ªhas been discussed and compared to determine the level of feasibility and operational features of technological support necessary to implement the site-linked BIM model. A case study based on the design and construction of the WPI Recreational & Sports Center, currently under construction, was developed to explore and understand the details that are involved in creating a new site model and to link it with the existing 3D building model. What has been learned from the analysis of this case study is presented, discussed and analyzed in terms of benefits and limitations. Recommendations for future extensions from both the research aspect and the technology support aspect finally presented. These include the creation of 3D BIM Campus Map, which is one site model with several building models placed on it to facilitate future planning of new building and/or maintenance and operation of the current buildings and campus infrastructure.   "

Made available in DSpace on 2015-03-05T18:11:59Z (GMT). No. of bitstreams: 0 Previous issue date: 15 / Nenhuma / Este trabalho tem por objetivos estudar o contexto de produção de linguagem no ensino e aprendizagem de Língua Portuguesa em uma situação particular de uso e manutenção de um site educacional, levantando implicações sobre o ensino e aprendizagem nessa situação; analisar e discutir as ações tecnológicas dos alunos, ou interatividade, num ambiente informatizado, visando teorizar sobre o contexto informatizado de produção de linguagem, a partir da delimitação das ações tecnológicas do aluno nesse contexto. Como teorias para o estudo estão a ação na perspectiva de Paul Ricoeur (1988 e 1991) e Jean-Paul Bronckart (1999); o contexto de produção de linguagem segundo Bronckart (2006) e o conceito de desenvolvimento, trabalhado por Vygotsky (1988 e 1994), priorizando a zona de desenvolvimento proximal. A pesquisa foi delimitada a oficinas para desenvolvimento de um site educacional, realizadas pela pesquisadora na Escola Municipal de Ensino Fundamental Incompleto Doutor Alfredo Bemfica Filho na cidade de Novo Hamburg

1: Por este livro passam outros. 1.1: Que são teses e dissertações senão livros? 1.2: O livro como um lugar, não um livro objeto, mas enquanto meio, programa. 2: Um corpo cujo contorno renega seu papel continente, sempre apontando sua razão de existir para além de seus limites. 3: Uma aproximação mimética, na medida em que se utiliza das possibilidades dispostas, categorias e ítens sobressalentes de um dispositivo opaco: a nota de rodapé, o registro fotográfico, a escrita formatada, a descrição como maneira, a pretensão de distância reflexiva, a objetividade anunciada em resumos. 4: O resgate suspeitoso de um mito de origem: a inserção da produção de arte no campo de erudição acadêmico sem que a primeira tivesse de se submeter ao segundo, (daí a noção de hóspede tomada de Duchamp, e como consequência sua dimensão fantasmática). 3. 1: Costurar tudo pela narrativa de um sujeito que a um só tempo analisa, olha e é visto, predicado. 5: Manipular os dados, e por isso, admitir a manipulação como método científico. Então afirmar tudo como num Tratado. 3.2: Foi dado um aviso: \"descrever muitas vezes nos desobriga de explicar\". Mas veio demasiado tarde: já se havia optado, como estratégia, pela distração. 5. 1: Propor a leitura, implicada em sua ficção, de uma linha de pesquisa e o que esta guarda de específico - quem sabe um manual, no sentido de que o livro pode-se ter em mãos. Mas nem vale fazer caso desta introdução. Resumo nenhum abre mão de sua pretensão de roteiro conciso que dispensa o que finge anunciar, o parodiar de mais uma Tese. / If the images give in to the association urge that gives them impulse, without the bond that condemns them to the shroud of ordinate thought. If writing renounces the imposed duty of servant to the ideas. If the footnotes leak through the page, allowing the book\'s layout to breath. If translation is confronted by treason, we assume this treason as creation and books cease to disguise as mere support for content: \"By this book elapse others. What are thesis and dissertations, but books?\" What would then be necessary to grant this Thesis, despite its uncommon format, any trace of respectability? In different languages, the political pretensions which are close to us sound like the resume of a fairy tale: \"In a far a way University, ranked first amidst those from Cruzeiro do Sul, there is a singular line of research, understood by us as a place among others...\" But it might come handy since we still defend fictional grounds as something proper of our condition. A matter of resistance to the appropriation faced by our writings to strange purposes -- although our purpose itself might be the strange one. To quote academics as writers, to quote Bataille`s Story of the Eye and, without notice, to grant the story a scientific status. To quote fiction itself and the wealthiest concepts in the most readable way, keeping its improper spelling: Concept. And thinking in a pragmatic way, how glamorous wouldn\'t be a well formulated monograph -- extended linearly and permeated with reflections proper to its medium -- named with an easily translatable title: Thesis as Site?

No description available.

Submitted by SILVIO LUIS AGOSTINHO DOS SANTOS null (silvioluis_26@hotmail.com) on 2017-03-06T19:50:30Z No. of bitstreams: 1 Versão Final da Dissertação do Mestrado de Silvio Luis Agostinho dos Santos.pdf: 3738913 bytes, checksum: bca1c0bfeff575f21eed73a5fa2cc3d4 (MD5) / Approved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2017-03-10T15:02:10Z (GMT) No. of bitstreams: 1 santos_sla_me_prud.pdf: 3738913 bytes, checksum: bca1c0bfeff575f21eed73a5fa2cc3d4 (MD5) / Made available in DSpace on 2017-03-10T15:02:10Z (GMT). No. of bitstreams: 1 santos_sla_me_prud.pdf: 3738913 bytes, checksum: bca1c0bfeff575f21eed73a5fa2cc3d4 (MD5) Previous issue date: 2017-02-23 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Na presente dissertação, descrevemos uma metodologia de ensino de Física que vem sendo implementada no Ensino Médio na ETEC Profº Eudécio Luiz Vicente localizada na cidade de Adamantina, SP. A proposta valoriza a compreensão da ciência como produção humana e fundamenta o processo ensino de uma aprendizagem significativa de Ausubel e na atividade experimental, de modo a articular o conhecimento formal da Física com os saberes do aluno. Em comparação com o chamado ensino tradicional, o que se propõe é um ensino mais atraente para os alunos, com ênfase na compreensão dos conceitos físicos e na relação destes com acontecimentos e fatos do dia a dia. Neste trabalho é apresentada uma proposta de inserção de experimentos de baixo custo e fácil acesso, no ensino de Física. Durante sua execução os alunos perceberam que os experimentos, aliados à analogia dos acontecimentos cotidianos, permitem a facilitação da construção do conhecimento. Foram realizados experimentos de Física, em que o aprendiz pudesse alcançar a compreensão das Leis que regem: a Mecânica, na Óptica, na Termologia, na Eletricidade, Magnetismo e no Eletromagnetismo. No final foi feito a montagem de 03 Kits de Experimentos, 01 Kit para cada série do Ensino Médio. Foi construído, também, um site do qual traz várias informações pertinentes ao desenvolvimento do projeto. O envolvimento dos alunos foi, inicialmente, empolgante para a realização dos experimentos e o rendimento escolar e a satisfação dos alunos foi maior. / In the present work, we describe a Physics teaching methodology that has been implemented in high school in ETEC Prof. Eudécio Luiz Vicente located in Adamantina, SP. The proposal values the understanding of science as a human production and founded the teaching process of meaningful learning of Ausubel and experimental activity, in order to articulate the formal knowledge of physics with the student's knowledge. Compared to the so-called traditional teaching, what is proposed is a more attractive school for students with an emphasis on understanding of physical concepts and their relationship with events and facts of everyday life. This paper presents a low-cost experiments insertion proposal and easy access, the teaching of physics. During its execution the students realized that the experiments together with the analogy of everyday events, allows the facilitation of knowledge building. We conducted physics experiments, in which the apprentice reached the understanding of the laws governing: Mechanical, in optics, in Thermology in Electricity, Magnetism and electromagnetism. At the end we were made to mount 03 Experiment Kits, 01 Kit for each high school grade. It was built also a site which brings various information relevant to the development of the project. The involvement of students was initially exciting for the experiments and school performance and student satisfaction was higher.